Rroxscaffold_2G00091000

Carboxymethylenebutenolidase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0007174
Physical Location & Seq
Reverse (-)
12657505 .. 12660869
3365 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00091000.1

Sequence Viewer

Length: 861 bp
ATGCTAGCATCGGTTGCTGTAAATCTGAGATCAGCAGCTTCCAGAGCCTTCTCAAATTCTCTCCCAAACTCTACTTTCTCACTCGTACGGACTCTCCCTCCTCCTTCGCTCTCTCGGTTCTCGGTTCGATCCATGGCGGATTCTGCTGCCCCGTTCAAGAAAGTCCAAATCCAGAGAGACGATACTGCATTTGATGCATATGTTGTTGGCAAAGAAGGTGCTCCTGGGATTGTTGTAGTTCAAGAGTGGTGGGGTGTAGATTTTGAAATAAAGAACCATGCTGTCAAAATTTCTGAACTTGCTCCTGGATTCAAAGCACTTATCCCTGACTTGTATCGGGGAAAGGTTGGTTTGGATGTTGCAGAAGCACAACATTTGATGGAAGGTCTTGACTGGCAAGGTGCGGTAAAGGATATCCAGGCTTCAGTTAATTGGCTGAAAGCAAATGGTTCAAAGAAGGTTGGAGTTACTGGATTCTGCATGGGAGGTGCTCTCTCAATTGCAAGTTCTGTTTTGGTCCCTGAGGTCGATGCTGTTGTTGCATTTTATGGAATTCCATCATCAGAGCTTGCAGACCCTGCAAAAGCTAAGGCTCCTATTCAGGCTCATTTTGGAGAGCTTGATAGTTTTGTTGGCTTTTCAGATGTCACGGCTGCCAAGTCTTTGGAGGAGAAGCTGAAAGCATCAGGAGTCCCATATGAGGTGCACCTGTATCCAGGCAATGCCCATGCTTTCATGAATAGGTCTGAAGACGGTATCAAGCGCAGGAAAGGCATGGGAATGCCTGATGAGGATGAAGCGGCAGTTCAGCTCGCTTGGTCTCGCTTCCAGTCATGGATGACCCGCCATTTGTCTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00361 ko00364 ko00623 ko01100 ko01110 ko01120 ko01130 map00361 map00364 map00623 map01100 map01110 map01120 map01130
Pfam Domains
Protein Families
DLH

Protein Analysis

286

Amino Acids

30.8

Weight (kDa)

6.6

Isoelectric Point (pI)

31.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DLH PF01738 64 - 283 4.2e-58 Dienelactone hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 137, 404, 800, 844
AclWI GGATC 1 cut(s) 123
AcsI RAATTY 3 cut(s) 55, 288, 552
AcuI CTGAAG 2 cut(s) 408, 768
AfaI GTAC 1 cut(s) 87
AfiI CCNNNNNNNGG 1 cut(s) 700
AgsI TTSAA 5 cut(s) 157, 242, 266, 313, 453
AjnI CCWGG 4 cut(s) 223, 304, 417, 715
AluBI AGCT 6 cut(s) 38, 568, 587, 619, 676, 811
AluI AGCT 6 cut(s) 38, 568, 587, 619, 676, 811
Alw21I GWGCWC 3 cut(s) 223, 493, 708
Alw26I GTCTC 2 cut(s) 171, 825
Alw44I GTGCAC 1 cut(s) 704
AlwI GGATC 1 cut(s) 123
AlwNI CAGNNNCTG 1 cut(s) 578
ApaLI GTGCAC 1 cut(s) 704
ApeKI GCWGC 3 cut(s) 35, 146, 653
ApoI RAATTY 3 cut(s) 55, 288, 552
ArsI GACNNNNNNTTYG 1 cut(s) 832
AspLEI GCGC 1 cut(s) 765
AspS9I GGNCC 1 cut(s) 517
AsuNHI GCTAGC 1 cut(s) 4
AvaII GGWCC 1 cut(s) 517
AxyI CCTNAGG 1 cut(s) 522
BaeGI GKGCMC 1 cut(s) 708
BbsI GAAGAC 1 cut(s) 756
Bbv12I GWGCWC 3 cut(s) 223, 493, 708
BbvI GCAGC 3 cut(s) 47, 133, 640
BccI CCATC 2 cut(s) 373, 565
BceAI ACGGC 1 cut(s) 666
BciT130I CCWGG 4 cut(s) 225, 306, 419, 717
BciVI GTATCC 1 cut(s) 723
BcoDI GTCTC 2 cut(s) 171, 825
BfaI CTAG 1 cut(s) 5
BfuI GTATCC 1 cut(s) 723
BisI GCNGC 4 cut(s) 36, 147, 654, 801
BlsI GCNGC 4 cut(s) 37, 148, 655, 802
Bme1390I CCNGG 4 cut(s) 225, 306, 419, 717
Bme18I GGWCC 1 cut(s) 517
BmgT120I GGNCC 1 cut(s) 517
BmiI GGNNCC 2 cut(s) 519, 594
BmrFI CCNGG 4 cut(s) 225, 306, 419, 717
BmsI GCATC 4 cut(s) 17, 184, 520, 692
BmtI GCTAGC 1 cut(s) 8
BpiI GAAGAC 1 cut(s) 756
BplI GAGNNNNNCTC 2 cut(s) 477, 509
Bpu10I CCTNAGC 1 cut(s) 588
BsaI GGTCTC 1 cut(s) 825
BsaJI CCNNGG 2 cut(s) 132, 224
BsaXI ACNNNNNCTCC 4 cut(s) 78, 82, 108, 112
Bsc4I CCNNNNNNNGG 1 cut(s) 700
Bse1I ACTGG 3 cut(s) 398, 475, 829
Bse21I CCTNAGG 1 cut(s) 522
Bse3DI GCAATG 1 cut(s) 727
BseBI CCWGG 4 cut(s) 225, 306, 419, 717
BseDI CCNNGG 2 cut(s) 132, 224
BseGI GGATG 3 cut(s) 361, 799, 843
BseLI CCNNNNNNNGG 1 cut(s) 700
BseMI GCAATG 1 cut(s) 727
BseMII CTCAG 2 cut(s) 17, 513
BseNI ACTGG 3 cut(s) 398, 475, 829
BseRI GAGGAG 2 cut(s) 90, 683
BseSI GKGCMC 1 cut(s) 708
BseXI GCAGC 3 cut(s) 47, 133, 640
BsiHKAI GWGCWC 3 cut(s) 223, 493, 708
BsiWI CGTACG 1 cut(s) 85
BslFI GGGAC 2 cut(s) 503, 677
BslI CCNNNNNNNGG 1 cut(s) 700
BsmAI GTCTC 2 cut(s) 171, 825
BsmBI CGTCTC 1 cut(s) 171
BsmFI GGGAC 2 cut(s) 503, 677
BsmI GAATGC 1 cut(s) 786
Bso31I GGTCTC 1 cut(s) 825
Bsp1286I GDGCHC 3 cut(s) 223, 493, 708
Bsp143I GATC 2 cut(s) 29, 128
Bsp19I CCATGG 1 cut(s) 132
BspACI CCGC 4 cut(s) 137, 404, 800, 844
BspCNI CTCAG 2 cut(s) 18, 514
BspHI TCATGA 1 cut(s) 735
BspLI GGNNCC 2 cut(s) 519, 594
BspOI GCTAGC 1 cut(s) 8
BspPI GGATC 1 cut(s) 123
BspTNI GGTCTC 1 cut(s) 825
BsrDI GCAATG 1 cut(s) 727
BsrI ACTGG 3 cut(s) 398, 475, 829
BssECI CCNNGG 2 cut(s) 132, 224
BssMI GATC 2 cut(s) 29, 128
BssT1I CCWWGG 1 cut(s) 132
Bst2UI CCWGG 4 cut(s) 225, 306, 419, 717
Bst4CI ACNGT 1 cut(s) 755
BstAPI GCANNNNNTGC 3 cut(s) 14, 194, 578
BstC8I GCNNGC 3 cut(s) 6, 570, 813
BstDEI CTNAG 3 cut(s) 26, 522, 588
BstDSI CCRYGG 1 cut(s) 132
BstF5I GGATG 3 cut(s) 361, 799, 843
BstHHI GCGC 1 cut(s) 765
BstKTI GATC 2 cut(s) 32, 131
BstMAI GTCTC 2 cut(s) 171, 825
BstMBI GATC 2 cut(s) 29, 128
BstMWI GCNNNNNNNGC 7 cut(s) 14, 44, 143, 194, 539, 578, 771
BstNI CCWGG 4 cut(s) 225, 306, 419, 717
BstSCI CCNGG 4 cut(s) 223, 304, 417, 715
BstSLI GKGCMC 1 cut(s) 708
BstV1I GCAGC 3 cut(s) 47, 133, 640
BstV2I GAAGAC 1 cut(s) 756
BstXI CCANNNNNNTGG 1 cut(s) 664
Bsu36I CCTNAGG 1 cut(s) 522
BsuI GTATCC 1 cut(s) 723
BtgI CCRYGG 1 cut(s) 132
BtsCI GGATG 3 cut(s) 361, 799, 843
Cac8I GCNNGC 3 cut(s) 6, 570, 813
CaiI CAGNNNCTG 1 cut(s) 578
CciI TCATGA 1 cut(s) 735
CfoI GCGC 1 cut(s) 765
Cfr13I GGNCC 1 cut(s) 517
Csp6I GTAC 1 cut(s) 86
CspCI CAANNNNNGTGG 2 cut(s) 230, 265
CviAII CATG 7 cut(s) 133, 278, 481, 728, 736, 775, 834
CviQI GTAC 1 cut(s) 86
DdeI CTNAG 3 cut(s) 26, 522, 588
DpnI GATC 2 cut(s) 31, 130
DpnII GATC 2 cut(s) 29, 128
EciI GGCGGA 1 cut(s) 152
Eco130I CCWWGG 1 cut(s) 132
Eco31I GGTCTC 1 cut(s) 825
Eco32I GATATC 1 cut(s) 415
Eco47I GGWCC 1 cut(s) 517
Eco57I CTGAAG 2 cut(s) 408, 768
Eco81I CCTNAGG 1 cut(s) 522
EcoRI GAATTC 1 cut(s) 552
EcoRII CCWGG 4 cut(s) 223, 304, 417, 715
EcoRV GATATC 1 cut(s) 415
EcoT14I CCWWGG 1 cut(s) 132
EcoT22I ATGCAT 1 cut(s) 199
ErhI CCWWGG 1 cut(s) 132
Esp3I CGTCTC 1 cut(s) 171
FaeI CATG 7 cut(s) 136, 281, 484, 731, 739, 778, 837
FaqI GGGAC 2 cut(s) 503, 677
FatI CATG 7 cut(s) 132, 277, 480, 727, 735, 774, 833
FauI CCCGC 1 cut(s) 851
FauNDI CATATG 2 cut(s) 199, 697
Fnu4HI GCNGC 4 cut(s) 36, 147, 654, 801
FokI GGATG 3 cut(s) 368, 806, 850
Fsp4HI GCNGC 4 cut(s) 36, 147, 654, 801
FspBI CTAG 1 cut(s) 5
GlaI GCGC 1 cut(s) 764
GluI GCNGC 4 cut(s) 36, 147, 654, 801
HhaI GCGC 1 cut(s) 765
Hin1II CATG 7 cut(s) 136, 281, 484, 731, 739, 778, 837
Hin6I GCGC 1 cut(s) 763
HinP1I GCGC 1 cut(s) 763
HinfI GANTC 5 cut(s) 91, 140, 309, 474, 690
Hpy166II GTNNAC 1 cut(s) 706
Hpy188I TCNGA 5 cut(s) 27, 295, 565, 643, 748
Hpy188III TCNNGA 7 cut(s) 42, 157, 172, 242, 389, 687, 736
Hpy8I GTNNAC 1 cut(s) 706
HpyAV CCTTC 5 cut(s) 58, 114, 209, 377, 451
HpyCH4III ACNGT 1 cut(s) 755
HpyCH4V TGCA 9 cut(s) 188, 197, 362, 480, 503, 542, 572, 581, 706
HpyF10VI GCNNNNNNNGC 7 cut(s) 14, 44, 143, 194, 539, 578, 771
HpyF3I CTNAG 3 cut(s) 26, 522, 588
Hsp92II CATG 7 cut(s) 136, 281, 484, 731, 739, 778, 837
HspAI GCGC 1 cut(s) 763
Kzo9I GATC 2 cut(s) 29, 128
LmnI GCTCC 3 cut(s) 226, 307, 598
Lsp1109I GCAGC 3 cut(s) 47, 133, 640
LweI GCATC 4 cut(s) 17, 184, 520, 692
MaeI CTAG 1 cut(s) 5
MaeIII GTNAC 2 cut(s) 466, 646
MalI GATC 2 cut(s) 31, 130
MboI GATC 2 cut(s) 29, 128
MboII GAAGA 1 cut(s) 761
MfeI CAATTG 1 cut(s) 498
MhlI GDGCHC 3 cut(s) 223, 493, 708
MluCI AATT 5 cut(s) 55, 288, 430, 498, 552
MlyI GAGTC 2 cut(s) 85, 699
MmeI TCCRAC 1 cut(s) 442
MnlI CCTC 7 cut(s) 108, 111, 479, 517, 661, 694, 784
Mph1103I ATGCAT 1 cut(s) 199
MseI TTAA 2 cut(s) 429, 859
MslI CAYNNNNRTG 1 cut(s) 779
MspR9I CCNGG 4 cut(s) 225, 306, 419, 717
MunI CAATTG 1 cut(s) 498
Mva1269I GAATGC 1 cut(s) 786
MvaI CCWGG 4 cut(s) 225, 306, 419, 717
MwoI GCNNNNNNNGC 7 cut(s) 14, 44, 143, 194, 539, 578, 771
NcoI CCATGG 1 cut(s) 132
NdeI CATATG 2 cut(s) 199, 697
NdeII GATC 2 cut(s) 29, 128
NheI GCTAGC 1 cut(s) 4
NlaIII CATG 7 cut(s) 136, 281, 484, 731, 739, 778, 837
NlaIV GGNNCC 2 cut(s) 519, 594
NmuCI GTSAC 1 cut(s) 646
NsiI ATGCAT 1 cut(s) 199
PagI TCATGA 1 cut(s) 735
PctI GAATGC 1 cut(s) 786
PfeI GAWTC 3 cut(s) 140, 309, 474
Pfl23II CGTACG 1 cut(s) 85
PfoI TCCNGGA 1 cut(s) 304
PkrI GCNGC 4 cut(s) 37, 148, 655, 802
PleI GAGTC 2 cut(s) 85, 698
PpsI GAGTC 2 cut(s) 85, 698
Psp6I CCWGG 4 cut(s) 223, 304, 417, 715
PspGI CCWGG 4 cut(s) 223, 304, 417, 715
PspLI CGTACG 1 cut(s) 85
PspN4I GGNNCC 2 cut(s) 519, 594
PspPI GGNCC 1 cut(s) 517
PstNI CAGNNNCTG 1 cut(s) 578
RsaI GTAC 1 cut(s) 87
RsaNI GTAC 1 cut(s) 86
RseI CAYNNNNRTG 1 cut(s) 779
SaqAI TTAA 2 cut(s) 429, 859
SatI GCNGC 4 cut(s) 36, 147, 654, 801
Sau3AI GATC 2 cut(s) 29, 128
Sau96I GGNCC 1 cut(s) 517
SchI GAGTC 2 cut(s) 85, 699
ScrFI CCNGG 4 cut(s) 225, 306, 419, 717
SduI GDGCHC 3 cut(s) 223, 493, 708
SfaNI GCATC 4 cut(s) 17, 184, 520, 692
SinI GGWCC 1 cut(s) 517
SmiMI CAYNNNNRTG 1 cut(s) 779
Sse9I AATT 5 cut(s) 55, 288, 430, 498, 552
SsiI CCGC 4 cut(s) 137, 404, 800, 844
SspMI CTAG 1 cut(s) 5
StyD4I CCNGG 4 cut(s) 223, 304, 417, 715
StyI CCWWGG 1 cut(s) 132
TaaI ACNGT 1 cut(s) 755
TaqI TCGA 2 cut(s) 127, 528
TasI AATT 5 cut(s) 55, 288, 430, 498, 552
TauI GCSGC 1 cut(s) 803
TfiI GAWTC 3 cut(s) 140, 309, 474
Tru1I TTAA 2 cut(s) 429, 859
Tru9I TTAA 2 cut(s) 429, 859
TseFI GTSAC 1 cut(s) 646
TseI GCWGC 3 cut(s) 35, 146, 653
Tsp45I GTSAC 1 cut(s) 646
TspDTI ATGAA 3 cut(s) 724, 752, 810
TspGWI ACGGA 1 cut(s) 103
VneI GTGCAC 1 cut(s) 704
VpaK11BI GGWCC 1 cut(s) 517
XapI RAATTY 3 cut(s) 55, 288, 552
XspI CTAG 1 cut(s) 5
Zsp2I ATGCAT 1 cut(s) 199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.