FvH4_6g43260

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0015671
Physical Location & Seq
Forward (+)
33518048 .. 33520823
2776 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g43260.t1

Sequence Viewer

Length: 888 bp
ATGGAGGGTGAAGCCGTGGCCGCAGCTGTCTCAGATCTCACTCCTCTGCTTGCCCTCACCCTCCTCCTTAATGGAGTTCAGCCAGTCTTGTCCGGTGTTGCTGTTGGGTATGGATGGCAAGCTCGGTGCCCTTCTTGGATTTTACTTCGACTTTGGTGCTATGGGAATATGGTTAGGGATAATGGCAAGTACGTTGATGCAAACAATCATTCTATTATGGATCACAATCCGAACGGACTGGATGAAGGAGAACTCAAAGAAGTTACAGCTAGTGAAAATCAGTTCAGGGGTAAATACGTCTTTTTATCACCCAGCTTATTTAAGGGGAATCCTAGGTTTTCGGGTTCTTCATTTTCTCGGCCGCCAACTGAAATGCCCAGTTTTTTGGTCGACCCGACCCGGACGAGACGCAGGCGGATTCCGTCGCCTTCCTTCCACCACTGGACGCCAGGCTGGTGGCGTTCAGAATATCGCCATGAGCTCGTTGCTTGCTGTTTTAATTCAGAATGGTTTTGGGATGGGAGGGATTGGAAAGAAAAGAAGAGTTTTTGCATCAAGTCGAGTAGGTCGTACTGGCAGAATAATCGAGTTGCAGAACAAGTTGCAGGTGACCTCATACTTCGAATGACAAACACACAGAGAAAAAATGGCTCCAAATTGCAAGGTTACCAGTTTGGTAAGACAGTTGCCAGGGGTTCTACTGCCAAGGTCAAGAAATGCATACATGTTGCAACTGGACAAACCGTGGGAATAAAAATATTGTCATTCAAAGGATTTCCAAAAATTAATCATGGGGCTCTAGAGAAGTTTAAGAAGAGAGTACAAAGAGAGATTTTTGTGGCACAGCATTTGAAGCACTCAAATGTGATGACTTGTTATGAGGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

296

Amino Acids

33.57

Weight (kDa)

9.78

Isoelectric Point (pI)

28.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 223 - 294 2.4e-06 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 596
Acc36I ACCTGC 1 cut(s) 596
AccB1I GGYRCC 1 cut(s) 126
AccI GTMKAC 1 cut(s) 390
AciI CCGC 3 cut(s) 21, 362, 415
AclWI GGATC 1 cut(s) 228
AcoI YGGCCR 2 cut(s) 18, 359
AcyI GRCGYC 1 cut(s) 446
AfaI GTAC 3 cut(s) 191, 572, 822
AflIII ACRYGT 1 cut(s) 724
AgsI TTSAA 2 cut(s) 769, 853
AjnI CCWGG 2 cut(s) 448, 689
AluBI AGCT 5 cut(s) 26, 122, 269, 315, 481
AluI AGCT 5 cut(s) 26, 122, 269, 315, 481
Alw21I GWGCWC 1 cut(s) 483
Alw26I GTCTC 2 cut(s) 34, 400
AlwI GGATC 1 cut(s) 228
AoxI GGCC 2 cut(s) 18, 359
ApeKI GCWGC 1 cut(s) 23
AseI ATTAAT 1 cut(s) 786
AspA2I CCTAGG 1 cut(s) 332
AsuC2I CCSGG 1 cut(s) 400
AsuHPI GGTGA 4 cut(s) 20, 49, 300, 620
AsuII TTCGAA 1 cut(s) 622
AvrII CCTAGG 1 cut(s) 332
BaeGI GKGCMC 1 cut(s) 131
BanI GGYRCC 1 cut(s) 126
BanII GRGCYC 2 cut(s) 483, 799
Bbv12I GWGCWC 1 cut(s) 483
BbvI GCAGC 1 cut(s) 35
BccI CCATC 2 cut(s) 108, 512
BcgI CGANNNNNNTGC 4 cut(s) 138, 172, 566, 600
BciT130I CCWGG 2 cut(s) 450, 691
BcnI CCSGG 1 cut(s) 400
BcoDI GTCTC 2 cut(s) 34, 400
BfaI CTAG 3 cut(s) 270, 333, 800
BfuAI ACCTGC 1 cut(s) 596
BglII AGATCT 1 cut(s) 34
BisI GCNGC 3 cut(s) 21, 24, 362
BlnI CCTAGG 1 cut(s) 332
BlsI GCNGC 3 cut(s) 22, 25, 363
Bme1390I CCNGG 3 cut(s) 400, 450, 691
BmiI GGNNCC 2 cut(s) 128, 652
BmrFI CCNGG 3 cut(s) 400, 450, 691
BmrI ACTGGG 1 cut(s) 372
BmsI GCATC 2 cut(s) 187, 561
BmuI ACTGGG 1 cut(s) 372
Bpu14I TTCGAA 1 cut(s) 622
BpuMI CCSGG 1 cut(s) 400
BsaBI GATNNNNATC 1 cut(s) 225
BsaHI GRCGYC 1 cut(s) 446
BsaJI CCNNGG 5 cut(s) 15, 332, 690, 705, 744
BsaWI WCCGGW 1 cut(s) 92
Bse1I ACTGG 7 cut(s) 83, 243, 378, 446, 578, 670, 739
Bse8I GATNNNNATC 1 cut(s) 225
BseBI CCWGG 2 cut(s) 450, 691
BseDI CCNNGG 5 cut(s) 15, 332, 690, 705, 744
BseGI GGATG 3 cut(s) 119, 247, 523
BseJI GATNNNNATC 1 cut(s) 225
BseMII CTCAG 1 cut(s) 45
BseNI ACTGG 7 cut(s) 83, 243, 378, 446, 578, 670, 739
BseRI GAGGAG 2 cut(s) 33, 53
BseSI GKGCMC 1 cut(s) 131
BseX3I CGGCCG 1 cut(s) 359
BseXI GCAGC 1 cut(s) 35
BseYI CCCAGC 1 cut(s) 311
Bsh1285I CGRYCG 1 cut(s) 362
BshFI GGCC 2 cut(s) 20, 361
BshNI GGYRCC 1 cut(s) 126
BsiEI CGRYCG 1 cut(s) 362
BsiHKAI GWGCWC 1 cut(s) 483
BsiSI CCGG 2 cut(s) 93, 400
BsmAI GTCTC 2 cut(s) 34, 400
BsmBI CGTCTC 1 cut(s) 400
BsnI GGCC 2 cut(s) 20, 361
Bsp119I TTCGAA 1 cut(s) 622
Bsp1286I GDGCHC 3 cut(s) 131, 483, 799
Bsp143I GATC 2 cut(s) 34, 220
BspACI CCGC 3 cut(s) 21, 362, 415
BspANI GGCC 2 cut(s) 20, 361
BspCNI CTCAG 1 cut(s) 44
BspLI GGNNCC 2 cut(s) 128, 652
BspMI ACCTGC 1 cut(s) 596
BspPI GGATC 1 cut(s) 228
BspT104I TTCGAA 1 cut(s) 622
BspT107I GGYRCC 1 cut(s) 126
BsrI ACTGG 7 cut(s) 83, 243, 378, 446, 578, 670, 739
BssECI CCNNGG 5 cut(s) 15, 332, 690, 705, 744
BssMI GATC 2 cut(s) 34, 220
BssNI GRCGYC 1 cut(s) 446
BssT1I CCWWGG 2 cut(s) 332, 705
Bst2UI CCWGG 2 cut(s) 450, 691
Bst4CI ACNGT 2 cut(s) 685, 745
Bst6I CTCTTC 2 cut(s) 536, 809
BstACI GRCGYC 1 cut(s) 446
BstBI TTCGAA 1 cut(s) 622
BstC8I GCNNGC 4 cut(s) 51, 120, 413, 490
BstDEI CTNAG 1 cut(s) 31
BstDSI CCRYGG 2 cut(s) 15, 744
BstEII GGTNACC 2 cut(s) 608, 665
BstF5I GGATG 3 cut(s) 119, 247, 523
BstKTI GATC 2 cut(s) 37, 223
BstMAI GTCTC 2 cut(s) 34, 400
BstMBI GATC 2 cut(s) 34, 220
BstMCI CGRYCG 1 cut(s) 362
BstMWI GCNNNNNNNGC 2 cut(s) 20, 853
BstNI CCWGG 2 cut(s) 450, 691
BstNSI RCATGY 1 cut(s) 728
BstPI GGTNACC 2 cut(s) 608, 665
BstSCI CCNGG 3 cut(s) 398, 448, 689
BstSLI GKGCMC 1 cut(s) 131
BstV1I GCAGC 1 cut(s) 35
BstX2I RGATCY 1 cut(s) 34
BstXI CCANNNNNNTGG 2 cut(s) 385, 456
BstYI RGATCY 1 cut(s) 34
BstZI CGGCCG 1 cut(s) 359
BsuRI GGCC 2 cut(s) 20, 361
BtgI CCRYGG 2 cut(s) 15, 744
BtsCI GGATG 3 cut(s) 119, 247, 523
BtsIMutI CAGTG 1 cut(s) 439
BveI ACCTGC 1 cut(s) 596
Cac8I GCNNGC 4 cut(s) 51, 120, 413, 490
CseI GACGC 2 cut(s) 417, 454
Csp6I GTAC 3 cut(s) 190, 571, 821
CviAII CATG 3 cut(s) 476, 725, 791
CviQI GTAC 3 cut(s) 190, 571, 821
DdeI CTNAG 1 cut(s) 31
DpnI GATC 2 cut(s) 36, 222
DpnII GATC 2 cut(s) 34, 220
EaeI YGGCCR 2 cut(s) 18, 359
EagI CGGCCG 1 cut(s) 359
Eam1104I CTCTTC 2 cut(s) 536, 809
EarI CTCTTC 2 cut(s) 536, 809
EciI GGCGGA 1 cut(s) 430
Ecl136II GAGCTC 1 cut(s) 481
EclXI CGGCCG 1 cut(s) 359
Eco130I CCWWGG 2 cut(s) 332, 705
Eco24I GRGCYC 2 cut(s) 483, 799
Eco52I CGGCCG 1 cut(s) 359
Eco53kI GAGCTC 1 cut(s) 481
Eco91I GGTNACC 2 cut(s) 608, 665
EcoICRI GAGCTC 1 cut(s) 481
EcoO65I GGTNACC 2 cut(s) 608, 665
EcoRII CCWGG 2 cut(s) 448, 689
EcoT14I CCWWGG 2 cut(s) 332, 705
EcoT22I ATGCAT 1 cut(s) 722
EcoT38I GRGCYC 2 cut(s) 483, 799
ErhI CCWWGG 2 cut(s) 332, 705
Esp3I CGTCTC 1 cut(s) 400
FaeI CATG 3 cut(s) 479, 728, 794
FatI CATG 3 cut(s) 475, 724, 790
FblI GTMKAC 1 cut(s) 390
Fnu4HI GCNGC 3 cut(s) 21, 24, 362
FokI GGATG 3 cut(s) 126, 254, 530
FriOI GRGCYC 2 cut(s) 483, 799
Fsp4HI GCNGC 3 cut(s) 21, 24, 362
FspBI CTAG 3 cut(s) 270, 333, 800
GluI GCNGC 3 cut(s) 21, 24, 362
GsaI CCCAGC 1 cut(s) 315
HaeIII GGCC 2 cut(s) 20, 361
HapII CCGG 2 cut(s) 93, 400
HgaI GACGC 2 cut(s) 417, 454
Hin1I GRCGYC 1 cut(s) 446
Hin1II CATG 3 cut(s) 479, 728, 794
HincII GTYRAC 1 cut(s) 391
HindII GTYRAC 1 cut(s) 391
HinfI GANTC 2 cut(s) 328, 418
HpaII CCGG 2 cut(s) 93, 400
HphI GGTGA 4 cut(s) 20, 49, 300, 620
Hpy166II GTNNAC 1 cut(s) 391
Hpy188I TCNGA 4 cut(s) 34, 231, 466, 505
Hpy188III TCNNGA 2 cut(s) 712, 800
Hpy8I GTNNAC 1 cut(s) 391
Hpy99I CGWCG 1 cut(s) 427
HpyAV CCTTC 4 cut(s) 141, 239, 438, 442
HpyCH4III ACNGT 2 cut(s) 685, 745
HpyCH4IV ACGT 2 cut(s) 192, 297
HpyCH4V TGCA 7 cut(s) 200, 552, 593, 605, 661, 720, 731
HpyF10VI GCNNNNNNNGC 2 cut(s) 20, 853
HpyF3I CTNAG 1 cut(s) 31
HpySE526I ACGT 2 cut(s) 192, 297
Hsp92I GRCGYC 1 cut(s) 446
Hsp92II CATG 3 cut(s) 479, 728, 794
Kzo9I GATC 2 cut(s) 34, 220
LmnI GCTCC 1 cut(s) 656
Lsp1109I GCAGC 1 cut(s) 35
LweI GCATC 2 cut(s) 187, 561
MaeI CTAG 3 cut(s) 270, 333, 800
MaeII ACGT 2 cut(s) 192, 297
MaeIII GTNAC 3 cut(s) 262, 608, 665
MalI GATC 2 cut(s) 36, 222
MboI GATC 2 cut(s) 34, 220
MboII GAAGA 3 cut(s) 339, 553, 826
MflI RGATCY 1 cut(s) 34
MhlI GDGCHC 3 cut(s) 131, 483, 799
MluCI AATT 3 cut(s) 499, 656, 783
MnlI CCTC 7 cut(s) 54, 65, 71, 74, 516, 623, 874
Mph1103I ATGCAT 1 cut(s) 722
MseI TTAA 5 cut(s) 69, 321, 498, 786, 810
MslI CAYNNNNRTG 1 cut(s) 861
MspA1I CMGCKG 1 cut(s) 26
MspI CCGG 2 cut(s) 93, 400
MspR9I CCNGG 3 cut(s) 400, 450, 691
MvaI CCWGG 2 cut(s) 450, 691
MwoI GCNNNNNNNGC 2 cut(s) 20, 853
NciI CCSGG 1 cut(s) 400
NdeII GATC 2 cut(s) 34, 220
NlaIII CATG 3 cut(s) 479, 728, 794
NlaIV GGNNCC 2 cut(s) 128, 652
NmeAIII GCCGAG 1 cut(s) 337
NmuCI GTSAC 1 cut(s) 608
NsiI ATGCAT 1 cut(s) 722
NspI RCATGY 1 cut(s) 728
NspV TTCGAA 1 cut(s) 622
PaqCI CACCTGC 1 cut(s) 596
PciI ACATGT 1 cut(s) 724
PcsI WCGNNNNNNNCGW 1 cut(s) 566
PfeI GAWTC 2 cut(s) 328, 418
PkrI GCNGC 3 cut(s) 22, 25, 363
PscI ACATGT 1 cut(s) 724
PshBI ATTAAT 1 cut(s) 786
Psp124BI GAGCTC 1 cut(s) 483
Psp6I CCWGG 2 cut(s) 448, 689
PspEI GGTNACC 2 cut(s) 608, 665
PspFI CCCAGC 1 cut(s) 311
PspGI CCWGG 2 cut(s) 448, 689
PspN4I GGNNCC 2 cut(s) 128, 652
PsuI RGATCY 1 cut(s) 34
PvuII CAGCTG 1 cut(s) 26
RsaI GTAC 3 cut(s) 191, 572, 822
RsaNI GTAC 3 cut(s) 190, 571, 821
RseI CAYNNNNRTG 1 cut(s) 861
SacI GAGCTC 1 cut(s) 483
SalI GTCGAC 1 cut(s) 389
SaqAI TTAA 5 cut(s) 69, 321, 498, 786, 810
SatI GCNGC 3 cut(s) 21, 24, 362
Sau3AI GATC 2 cut(s) 34, 220
ScrFI CCNGG 3 cut(s) 400, 450, 691
SduI GDGCHC 3 cut(s) 131, 483, 799
SfaNI GCATC 2 cut(s) 187, 561
SfuI TTCGAA 1 cut(s) 622
SmiMI CAYNNNNRTG 1 cut(s) 861
Sse9I AATT 3 cut(s) 499, 656, 783
SsiI CCGC 3 cut(s) 21, 362, 415
SspI AATATT 1 cut(s) 759
SspMI CTAG 3 cut(s) 270, 333, 800
SstI GAGCTC 1 cut(s) 483
StyD4I CCNGG 3 cut(s) 398, 448, 689
StyI CCWWGG 2 cut(s) 332, 705
TaaI ACNGT 2 cut(s) 685, 745
TaiI ACGT 2 cut(s) 195, 300
TaqI TCGA 5 cut(s) 148, 390, 560, 586, 622
TasI AATT 3 cut(s) 499, 656, 783
TatI WGTACW 1 cut(s) 820
TauI GCSGC 2 cut(s) 23, 364
TfiI GAWTC 2 cut(s) 328, 418
Tru1I TTAA 5 cut(s) 69, 321, 498, 786, 810
Tru9I TTAA 5 cut(s) 69, 321, 498, 786, 810
TscAI CASTG 1 cut(s) 446
TseFI GTSAC 1 cut(s) 608
TseI GCWGC 1 cut(s) 23
Tsp45I GTSAC 1 cut(s) 608
TspDTI ATGAA 2 cut(s) 258, 339
TspGWI ACGGA 2 cut(s) 249, 411
TspRI CASTG 1 cut(s) 446
VspI ATTAAT 1 cut(s) 786
XbaI TCTAGA 1 cut(s) 799
XceI RCATGY 1 cut(s) 728
XmaJI CCTAGG 1 cut(s) 332
XmiI GTMKAC 1 cut(s) 390
XspI CTAG 3 cut(s) 270, 333, 800
Zsp2I ATGCAT 1 cut(s) 722
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.