RLG00000021162

SNF1-related protein kinase catalytic subunit alpha

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0015671
Physical Location & Seq
Forward (+)
72489115 .. 72491764
2650 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021162

Sequence Viewer

Length: 861 bp
ATGGCTCCAAAATTGGAAGGTTACAAGTTTGGTGAGACAATTGCCAGGGGTTCTGGTGCCAAGTTGAAGAAAGCCATACATGTTGCAACAGGGCAGACCGTGGCTATCAAAATATTATCTTTTAAAGGATTTGCAAAAGTTAATCATGGGGCACTACAGAAATTTAAGAAGAGAGAGGACTATATTGCACAAAGAGGAGGGACATTAACTGAAGGGGAAGCTCGCAGATACTTCTGCCAGCTCATTCATGGTGTTGAGTATTGCCATAAACTCCTTTTGATTCATCGGGATTTGAAACCTGGAAATCTACTCTTGGATGAAACCAAAGAACATCTGAAAATAGCAGACTTTGGAATAAGCAATTATCTGACAGCTGATTTGACATTAAGTACCTATTGCGGAACAAATTTATATCAGGCTCAAGAGACAAGCAAAGATGGTACAAATTACAGTGGCCCAGAGGTTGATATCTACAGCTGTGGTGTAACACTTTACCACCTCTTATTCGGCTGCCCATCACTACGGAGCTTTCAATATGGCAGACTCTCTATGCCTCTGTTGGCCCCAGCAGATGCAGCAGATCTAATAAGTAGAATGACAGAATGGGAACCAAAGCAGCGTATCACCATCGGACAAATCAAGAACCATAGATGGTTTCAAGGTGGGGATCAAATGTTCTTAAATGATCCTCATGGCATTTCTGATCAGGGTATTGACAACAACATCCTATTGGAGACTATCAAGAAGACGCAATGTGAAAGAGAATATCTTATTCAGTCTCTAATTCAGGGGGAACACACAGAGGAAACCACCACATACAAGCTGCTAAAGAAGAAGAAGATTCGGGATGTTGAAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001101 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007275 GO:0007568 GO:0007584 GO:0008150 GO:0008152 GO:0009593 GO:0009594 GO:0009605 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009743 GO:0009755 GO:0009756 GO:0009791 GO:0009987 GO:0009991 GO:0010033 GO:0010050 GO:0010150 GO:0010182 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022414 GO:0022622 GO:0023052 GO:0031667 GO:0032501 GO:0032502 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0048364 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051606 GO:0051716 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071322 GO:0071396 GO:0071495 GO:0071704 GO:0080022 GO:0090693 GO:0097305 GO:0097306 GO:0099402 GO:0140096 GO:1901564 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
ko04068 ko04140 ko04150 ko04151 ko04152 ko04211 ko04213 ko04371 ko04530 ko04710 ko04714 ko04910 ko04920 ko04921 ko04922 ko04931 ko04932 ko05410 ko05418 map04068 map04140 map04150 map04151 map04152 map04211 map04213 map04371 map04530 map04710 map04714 map04910 map04920 map04921 map04922 map04931 map04932 map05410 map05418
Pfam Domains
Protein Families
KA1 Pkinase UBA

Protein Analysis

287

Amino Acids

32.33

Weight (kDa)

8.58

Isoelectric Point (pI)

23.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 49 - 213 1.7e-12 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 60 - 219 1.3e-36 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 56
AciI CCGC 1 cut(s) 399
AclWI GGATC 2 cut(s) 675, 680
AcsI RAATTY 2 cut(s) 161, 406
AcuI CTGAAG 1 cut(s) 231
AfaI GTAC 2 cut(s) 391, 442
AflIII ACRYGT 1 cut(s) 79
AgsI TTSAA 5 cut(s) 67, 295, 533, 659, 854
AjnI CCWGG 2 cut(s) 44, 298
AloI GAACNNNNNNTCC 2 cut(s) 659, 691
AluBI AGCT 6 cut(s) 221, 241, 374, 477, 528, 823
AluI AGCT 6 cut(s) 221, 241, 374, 477, 528, 823
Alw26I GTCTC 4 cut(s) 29, 419, 728, 783
AlwI GGATC 2 cut(s) 675, 680
AoxI GGCC 2 cut(s) 454, 561
ApeKI GCWGC 4 cut(s) 510, 575, 616, 823
ApoI RAATTY 2 cut(s) 161, 406
ArsI GACNNNNNNTTYG 2 cut(s) 361, 393
AspS9I GGNCC 2 cut(s) 455, 562
AsuHPI GGTGA 2 cut(s) 44, 616
BaeGI GKGCMC 1 cut(s) 154
BanI GGYRCC 1 cut(s) 56
BbsI GAAGAC 1 cut(s) 752
BbvI GCAGC 4 cut(s) 497, 587, 628, 810
BccI CCATC 4 cut(s) 431, 523, 635, 645
BciT130I CCWGG 2 cut(s) 46, 300
BclI TGATCA 1 cut(s) 703
BcoDI GTCTC 4 cut(s) 29, 419, 728, 783
BfmI CTRYAG 2 cut(s) 155, 472
BglII AGATCT 1 cut(s) 580
BisI GCNGC 4 cut(s) 511, 576, 617, 824
BlsI GCNGC 4 cut(s) 512, 577, 618, 825
Bme1390I CCNGG 2 cut(s) 46, 300
BmgT120I GGNCC 2 cut(s) 455, 562
BmiI GGNNCC 4 cut(s) 6, 58, 564, 609
BmrFI CCNGG 2 cut(s) 46, 300
BmsI GCATC 1 cut(s) 562
BpiI GAAGAC 1 cut(s) 752
BpuEI CTTGAG 1 cut(s) 405
BsaJI CCNNGG 2 cut(s) 45, 99
Bse3DI GCAATG 1 cut(s) 758
BseBI CCWGG 2 cut(s) 46, 300
BseDI CCNNGG 2 cut(s) 45, 99
BseGI GGATG 3 cut(s) 322, 723, 853
BseMI GCAATG 1 cut(s) 758
BseRI GAGGAG 1 cut(s) 210
BseSI GKGCMC 1 cut(s) 154
BseXI GCAGC 4 cut(s) 497, 587, 628, 810
BseYI CCCAGC 1 cut(s) 565
BshFI GGCC 2 cut(s) 456, 563
BshNI GGYRCC 1 cut(s) 56
BslFI GGGAC 1 cut(s) 214
BsmAI GTCTC 4 cut(s) 29, 419, 728, 783
BsmFI GGGAC 1 cut(s) 214
BsnI GGCC 2 cut(s) 456, 563
Bsp1286I GDGCHC 1 cut(s) 154
Bsp143I GATC 4 cut(s) 580, 667, 685, 703
BspACI CCGC 1 cut(s) 399
BspANI GGCC 2 cut(s) 456, 563
BspLI GGNNCC 4 cut(s) 6, 58, 564, 609
BspPI GGATC 2 cut(s) 675, 680
BspT107I GGYRCC 1 cut(s) 56
BsrDI GCAATG 1 cut(s) 758
BssECI CCNNGG 2 cut(s) 45, 99
BssMI GATC 4 cut(s) 580, 667, 685, 703
Bst2UI CCWGG 2 cut(s) 46, 300
Bst4CI ACNGT 2 cut(s) 100, 452
Bst6I CTCTTC 1 cut(s) 164
BstC8I GCNNGC 2 cut(s) 223, 239
BstDSI CCRYGG 1 cut(s) 99
BstF5I GGATG 3 cut(s) 322, 723, 853
BstKTI GATC 4 cut(s) 583, 670, 688, 706
BstMAI GTCTC 4 cut(s) 29, 419, 728, 783
BstMBI GATC 4 cut(s) 580, 667, 685, 703
BstMWI GCNNNNNNNGC 1 cut(s) 575
BstNI CCWGG 2 cut(s) 46, 300
BstNSI RCATGY 1 cut(s) 83
BstSCI CCNGG 2 cut(s) 44, 298
BstSFI CTRYAG 2 cut(s) 155, 472
BstSLI GKGCMC 1 cut(s) 154
BstV1I GCAGC 4 cut(s) 497, 587, 628, 810
BstV2I GAAGAC 1 cut(s) 752
BstX2I RGATCY 1 cut(s) 580
BstYI RGATCY 1 cut(s) 580
BsuRI GGCC 2 cut(s) 456, 563
BtgI CCRYGG 1 cut(s) 99
BtsCI GGATG 3 cut(s) 322, 723, 853
BtsIMutI CAGTG 1 cut(s) 457
Cac8I GCNNGC 2 cut(s) 223, 239
Cfr13I GGNCC 2 cut(s) 455, 562
CseI GACGC 1 cut(s) 757
Csp6I GTAC 2 cut(s) 390, 441
CviAII CATG 4 cut(s) 80, 146, 248, 692
CviQI GTAC 2 cut(s) 390, 441
DpnI GATC 4 cut(s) 582, 669, 687, 705
DpnII GATC 4 cut(s) 580, 667, 685, 703
DraI TTTAAA 1 cut(s) 124
Eam1104I CTCTTC 1 cut(s) 164
EarI CTCTTC 1 cut(s) 164
Eco32I GATATC 1 cut(s) 469
Eco57I CTGAAG 1 cut(s) 231
EcoRII CCWGG 2 cut(s) 44, 298
EcoRV GATATC 1 cut(s) 469
FaeI CATG 4 cut(s) 83, 149, 251, 695
FaqI GGGAC 1 cut(s) 214
FatI CATG 4 cut(s) 79, 145, 247, 691
FbaI TGATCA 1 cut(s) 703
Fnu4HI GCNGC 4 cut(s) 511, 576, 617, 824
FokI GGATG 2 cut(s) 329, 710
Fsp4HI GCNGC 4 cut(s) 511, 576, 617, 824
GluI GCNGC 4 cut(s) 511, 576, 617, 824
GsaI CCCAGC 1 cut(s) 569
HaeIII GGCC 2 cut(s) 456, 563
HgaI GACGC 1 cut(s) 757
Hin1II CATG 4 cut(s) 83, 149, 251, 695
HinfI GANTC 3 cut(s) 280, 543, 841
HphI GGTGA 2 cut(s) 44, 616
Hpy188I TCNGA 4 cut(s) 336, 369, 632, 703
Hpy188III TCNNGA 5 cut(s) 287, 422, 640, 742, 845
HpyAV CCTTC 2 cut(s) 11, 206
HpyCH4III ACNGT 2 cut(s) 100, 452
HpyCH4V TGCA 4 cut(s) 86, 134, 188, 575
HpyF10VI GCNNNNNNNGC 1 cut(s) 575
Hsp92II CATG 4 cut(s) 83, 149, 251, 695
Ksp22I TGATCA 1 cut(s) 703
Kzo9I GATC 4 cut(s) 580, 667, 685, 703
LmnI GCTCC 2 cut(s) 10, 525
Lsp1109I GCAGC 4 cut(s) 497, 587, 628, 810
LweI GCATC 1 cut(s) 562
MaeIII GTNAC 2 cut(s) 20, 484
MalI GATC 4 cut(s) 582, 669, 687, 705
MboI GATC 4 cut(s) 580, 667, 685, 703
MboII GAAGA 6 cut(s) 79, 181, 757, 844, 847, 850
MfeI CAATTG 1 cut(s) 39
MflI RGATCY 1 cut(s) 580
MhlI GDGCHC 1 cut(s) 154
MluCI AATT 7 cut(s) 11, 39, 161, 361, 406, 445, 783
MlyI GAGTC 1 cut(s) 537
MnlI CCTC 8 cut(s) 169, 188, 191, 454, 509, 564, 699, 796
MseI TTAA 6 cut(s) 123, 141, 165, 206, 386, 680
MspA1I CMGCKG 2 cut(s) 374, 477
MspR9I CCNGG 2 cut(s) 46, 300
MunI CAATTG 1 cut(s) 39
MvaI CCWGG 2 cut(s) 46, 300
MwoI GCNNNNNNNGC 1 cut(s) 575
NdeII GATC 4 cut(s) 580, 667, 685, 703
NlaIII CATG 4 cut(s) 83, 149, 251, 695
NlaIV GGNNCC 4 cut(s) 6, 58, 564, 609
NspI RCATGY 1 cut(s) 83
PciI ACATGT 1 cut(s) 79
PfeI GAWTC 2 cut(s) 280, 841
PkrI GCNGC 4 cut(s) 512, 577, 618, 825
PleI GAGTC 1 cut(s) 537
PpsI GAGTC 1 cut(s) 537
PscI ACATGT 1 cut(s) 79
Psp6I CCWGG 2 cut(s) 44, 298
PspFI CCCAGC 1 cut(s) 565
PspGI CCWGG 2 cut(s) 44, 298
PspN4I GGNNCC 4 cut(s) 6, 58, 564, 609
PspPI GGNCC 2 cut(s) 455, 562
PsuI RGATCY 1 cut(s) 580
PvuII CAGCTG 2 cut(s) 374, 477
RsaI GTAC 2 cut(s) 391, 442
RsaNI GTAC 2 cut(s) 390, 441
SaqAI TTAA 6 cut(s) 123, 141, 165, 206, 386, 680
SatI GCNGC 4 cut(s) 511, 576, 617, 824
Sau3AI GATC 4 cut(s) 580, 667, 685, 703
Sau96I GGNCC 2 cut(s) 455, 562
SchI GAGTC 1 cut(s) 537
ScrFI CCNGG 2 cut(s) 46, 300
SduI GDGCHC 1 cut(s) 154
SfaNI GCATC 1 cut(s) 562
SfcI CTRYAG 2 cut(s) 155, 472
SmlI CTYRAG 1 cut(s) 420
SmoI CTYRAG 1 cut(s) 420
Sse9I AATT 7 cut(s) 11, 39, 161, 361, 406, 445, 783
SsiI CCGC 1 cut(s) 399
SspI AATATT 1 cut(s) 114
StyD4I CCNGG 2 cut(s) 44, 298
TaaI ACNGT 2 cut(s) 100, 452
TasI AATT 7 cut(s) 11, 39, 161, 361, 406, 445, 783
TfiI GAWTC 2 cut(s) 280, 841
Tru1I TTAA 6 cut(s) 123, 141, 165, 206, 386, 680
Tru9I TTAA 6 cut(s) 123, 141, 165, 206, 386, 680
TscAI CASTG 1 cut(s) 457
TseI GCWGC 4 cut(s) 510, 575, 616, 823
TspDTI ATGAA 3 cut(s) 236, 272, 333
TspGWI ACGGA 1 cut(s) 538
TspRI CASTG 1 cut(s) 457
XapI RAATTY 2 cut(s) 161, 406
XceI RCATGY 1 cut(s) 83
XcmI CCANNNNNNNNNTGG 1 cut(s) 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.