Rh2DG565500

Adenylate sensor of SNF1-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0015671
Physical Location & Seq
Forward (+)
79544076 .. 79547794
3719 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG565500.1

Sequence Viewer

Length: 843 bp
ATGAAAAAAGAATGGCATTTGAAGCACAGAGAGATGTTTGTGGCAAAGCATTTGAAGCACCCAAATATTATGACTTGCTATATGGGGCAGATGTATAAAAAAAGGGCATATATTGTGATGGAATGGATGGAGGGTGGGAGTTTGGATGACTATCTTGCACAAAGAGGAGGGACATTAACTGAAGGGGAAGCTCGCAGATACTTCCGTCAGCTCATTCATGGTGTTGAGTATTGCCATAAACTCTTTTTGATTCATCGGGATTTGAAACCTGGAAATCTACTCTTGGATGAAACTAAAGAACATCTGAAAATAGCAGACTTTGGAATAAGCAATTATCTGACCGCTGATTTGACATTAAGTTCCTATTGCGGAACAAATTTCTCTCAGGCTCCCGAGATAAGCAAAGATGGTACAAATTACGTTGGCCCAGAGGTTGATATCTACAGCTGTGGTGTAACACTTTACCACCTCTTATTCGGCTGCCCATCACTACTGAGCTTTCAATATGGCAGACTCTCTATTCCTCAGTCGACCACAGCAGATCTAATACATAGAATGATAGCATGGGAACCAAAGGATCGTATCAACATCCGAGAAATCAAGAACCACAGATGGTTTCAAGGTTGGGATCAAATGTTCTTAAATGATCCTCATGGCATTTCTGATCAGGGTATTGACAACAACATCCTATTGGAGGCTATCAAGAAGATGCAATGTGAGAGGGAATATCTTATTCGGTCTCTAATTCAGGGGGAACACACAGGTGGGTTTTGGACATCAATAGTTGCTTTGGATTTCCACCCTTATTCACTCATTTTCTATTTAATTTTTTATCCTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004679 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006163 GO:0006164 GO:0006464 GO:0006468 GO:0006725 GO:0006753 GO:0006754 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007005 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009058 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009167 GO:0009168 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009314 GO:0009416 GO:0009453 GO:0009605 GO:0009628 GO:0009892 GO:0009893 GO:0009987 GO:0010468 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010646 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019222 GO:0019438 GO:0019538 GO:0019637 GO:0019693 GO:0019954 GO:0023052 GO:0031156 GO:0031285 GO:0032101 GO:0032104 GO:0032107 GO:0034641 GO:0034654 GO:0035556 GO:0036211 GO:0040011 GO:0042330 GO:0042331 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043900 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044464 GO:0045595 GO:0046034 GO:0046390 GO:0046483 GO:0046956 GO:0048518 GO:0048519 GO:0048583 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051716 GO:0052128 GO:0055086 GO:0060176 GO:0060255 GO:0065007 GO:0071704 GO:0071840 GO:0072521 GO:0072522 GO:0080134 GO:0080135 GO:0090407 GO:0140096 GO:1901135 GO:1901137 GO:1901261 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1903664 GO:1903665 GO:2000241
KEGG Pathways
Metabolic & Signaling
ko04068 ko04140 ko04150 ko04151 ko04152 ko04211 ko04213 ko04371 ko04530 ko04710 ko04714 ko04910 ko04920 ko04921 ko04922 ko04931 ko04932 ko05410 ko05418 map04068 map04140 map04150 map04151 map04152 map04211 map04213 map04371 map04530 map04710 map04714 map04910 map04920 map04921 map04922 map04931 map04932 map05410 map05418
Pfam Domains
Protein Families
AdenylateSensor Pkinase

Protein Analysis

280

Amino Acids

32.75

Weight (kDa)

6.92

Isoelectric Point (pI)

31.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 8 - 206 3.8e-48 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 9 - 159 8.5e-25 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 30 - 115 2.3e-07 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 530
AciI CCGC 2 cut(s) 342, 369
AclWI GGATC 3 cut(s) 585, 636, 641
AcsI RAATTY 1 cut(s) 376
AcuI CTGAAG 1 cut(s) 201
AfaI GTAC 1 cut(s) 412
AfiI CCNNNNNNNGG 1 cut(s) 694
AgsI TTSAA 5 cut(s) 22, 55, 265, 503, 620
AjnI CCWGG 1 cut(s) 268
AleI CACNNNNGTG 1 cut(s) 762
AloI GAACNNNNNNTCC 2 cut(s) 620, 652
AluBI AGCT 4 cut(s) 191, 211, 447, 498
AluI AGCT 4 cut(s) 191, 211, 447, 498
Alw26I GTCTC 1 cut(s) 744
AlwI GGATC 3 cut(s) 585, 636, 641
Ama87I CYCGRG 1 cut(s) 392
AoxI GGCC 1 cut(s) 424
ApeKI GCWGC 1 cut(s) 480
ApoI RAATTY 1 cut(s) 376
ArsI GACNNNNNNTTYG 2 cut(s) 331, 363
AspS9I GGNCC 1 cut(s) 425
AvaI CYCGRG 1 cut(s) 392
BaeI ACNNNNGTAYC 2 cut(s) 190, 223
BbvI GCAGC 1 cut(s) 467
BccI CCATC 5 cut(s) 112, 121, 401, 493, 606
BciT130I CCWGG 1 cut(s) 270
BclI TGATCA 1 cut(s) 664
BcoDI GTCTC 1 cut(s) 744
BfaI CTAG 1 cut(s) 837
BfmI CTRYAG 1 cut(s) 442
BglII AGATCT 1 cut(s) 541
BisI GCNGC 1 cut(s) 481
BlsI GCNGC 1 cut(s) 482
Bme1390I CCNGG 1 cut(s) 270
BmeT110I CYCGRG 1 cut(s) 392
BmgT120I GGNCC 1 cut(s) 425
BmiI GGNNCC 2 cut(s) 390, 570
BmrFI CCNGG 1 cut(s) 270
BmsI GCATC 1 cut(s) 699
BsaBI GATNNNNATC 1 cut(s) 150
BsaI GGTCTC 1 cut(s) 744
Bsc4I CCNNNNNNNGG 1 cut(s) 694
Bse3DI GCAATG 1 cut(s) 719
Bse8I GATNNNNATC 1 cut(s) 150
BseBI CCWGG 1 cut(s) 270
BseGI GGATG 5 cut(s) 132, 151, 292, 588, 684
BseJI GATNNNNATC 1 cut(s) 150
BseLI CCNNNNNNNGG 1 cut(s) 694
BseMI GCAATG 1 cut(s) 719
BseMII CTCAG 3 cut(s) 398, 485, 539
BseRI GAGGAG 1 cut(s) 180
BseXI GCAGC 1 cut(s) 467
BshFI GGCC 1 cut(s) 426
BsiHKCI CYCGRG 1 cut(s) 392
BslFI GGGAC 1 cut(s) 184
BslI CCNNNNNNNGG 1 cut(s) 694
BsmAI GTCTC 1 cut(s) 744
BsmFI GGGAC 1 cut(s) 184
BsnI GGCC 1 cut(s) 426
Bso31I GGTCTC 1 cut(s) 744
BsoBI CYCGRG 1 cut(s) 392
Bsp143I GATC 5 cut(s) 541, 577, 628, 646, 664
BspACI CCGC 2 cut(s) 342, 369
BspANI GGCC 1 cut(s) 426
BspCNI CTCAG 3 cut(s) 397, 486, 538
BspLI GGNNCC 2 cut(s) 390, 570
BspPI GGATC 3 cut(s) 585, 636, 641
BspTNI GGTCTC 1 cut(s) 744
BsrDI GCAATG 1 cut(s) 719
BssMI GATC 5 cut(s) 541, 577, 628, 646, 664
Bst2UI CCWGG 1 cut(s) 270
BstC8I GCNNGC 1 cut(s) 193
BstDEI CTNAG 3 cut(s) 384, 494, 525
BstENI CCTNNNNNAGG 1 cut(s) 692
BstF5I GGATG 5 cut(s) 132, 151, 292, 588, 684
BstKTI GATC 5 cut(s) 544, 580, 631, 649, 667
BstMAI GTCTC 1 cut(s) 744
BstMBI GATC 5 cut(s) 541, 577, 628, 646, 664
BstMWI GCNNNNNNNGC 2 cut(s) 22, 55
BstNI CCWGG 1 cut(s) 270
BstSCI CCNGG 1 cut(s) 268
BstSFI CTRYAG 1 cut(s) 442
BstV1I GCAGC 1 cut(s) 467
BstX2I RGATCY 1 cut(s) 541
BstYI RGATCY 1 cut(s) 541
BsuRI GGCC 1 cut(s) 426
BtsCI GGATG 5 cut(s) 132, 151, 292, 588, 684
Cac8I GCNNGC 1 cut(s) 193
Cfr13I GGNCC 1 cut(s) 425
Csp6I GTAC 1 cut(s) 411
CviAII CATG 3 cut(s) 218, 564, 653
CviJI RGCY 8 cut(s) 191, 211, 389, 426, 447, 480, 498, 698
CviKI_1 RGCY 8 cut(s) 191, 211, 389, 426, 447, 480, 498, 698
CviQI GTAC 1 cut(s) 411
DdeI CTNAG 3 cut(s) 384, 494, 525
DpnI GATC 5 cut(s) 543, 579, 630, 648, 666
DpnII GATC 5 cut(s) 541, 577, 628, 646, 664
Eco31I GGTCTC 1 cut(s) 744
Eco32I GATATC 1 cut(s) 439
Eco57I CTGAAG 1 cut(s) 201
Eco88I CYCGRG 1 cut(s) 392
EcoNI CCTNNNNNAGG 1 cut(s) 692
EcoRII CCWGG 1 cut(s) 268
EcoRV GATATC 1 cut(s) 439
FaeI CATG 3 cut(s) 221, 567, 656
FaqI GGGAC 1 cut(s) 184
FatI CATG 3 cut(s) 217, 563, 652
FbaI TGATCA 1 cut(s) 664
FblI GTMKAC 1 cut(s) 530
Fnu4HI GCNGC 1 cut(s) 481
FokI GGATG 5 cut(s) 139, 158, 299, 575, 671
Fsp4HI GCNGC 1 cut(s) 481
FspBI CTAG 1 cut(s) 837
GluI GCNGC 1 cut(s) 481
HaeIII GGCC 1 cut(s) 426
Hin1II CATG 3 cut(s) 221, 567, 656
HincII GTYRAC 1 cut(s) 531
HindII GTYRAC 1 cut(s) 531
HinfI GANTC 2 cut(s) 250, 513
Hpy166II GTNNAC 1 cut(s) 531
Hpy188I TCNGA 4 cut(s) 306, 339, 593, 664
Hpy188III TCNNGA 4 cut(s) 257, 392, 601, 703
Hpy8I GTNNAC 1 cut(s) 531
HpyAV CCTTC 1 cut(s) 176
HpyCH4IV ACGT 1 cut(s) 420
HpyCH4V TGCA 2 cut(s) 158, 712
HpyF10VI GCNNNNNNNGC 2 cut(s) 22, 55
HpyF3I CTNAG 3 cut(s) 384, 494, 525
HpySE526I ACGT 1 cut(s) 420
Hsp92II CATG 3 cut(s) 221, 567, 656
Ksp22I TGATCA 1 cut(s) 664
Kzo9I GATC 5 cut(s) 541, 577, 628, 646, 664
LmnI GCTCC 1 cut(s) 394
LpnPI CCDG 7 cut(s) 255, 282, 371, 441, 653, 734, 747
Lsp1109I GCAGC 1 cut(s) 467
LweI GCATC 1 cut(s) 699
MaeI CTAG 1 cut(s) 837
MaeII ACGT 1 cut(s) 420
MaeIII GTNAC 1 cut(s) 454
MalI GATC 5 cut(s) 543, 579, 630, 648, 666
MboI GATC 5 cut(s) 541, 577, 628, 646, 664
MboII GAAGA 1 cut(s) 718
MflI RGATCY 1 cut(s) 541
MluCI AATT 5 cut(s) 331, 376, 415, 744, 825
MlyI GAGTC 1 cut(s) 507
MnlI CCTC 9 cut(s) 124, 158, 161, 424, 479, 534, 660, 688, 714
MseI TTAA 4 cut(s) 176, 356, 641, 824
MslI CAYNNNNRTG 1 cut(s) 762
MspA1I CMGCKG 2 cut(s) 344, 447
MspR9I CCNGG 1 cut(s) 270
MvaI CCWGG 1 cut(s) 270
MwoI GCNNNNNNNGC 2 cut(s) 22, 55
NdeII GATC 5 cut(s) 541, 577, 628, 646, 664
NlaIII CATG 3 cut(s) 221, 567, 656
NlaIV GGNNCC 2 cut(s) 390, 570
OliI CACNNNNGTG 1 cut(s) 762
PfeI GAWTC 1 cut(s) 250
PkrI GCNGC 1 cut(s) 482
PleI GAGTC 1 cut(s) 507
PpsI GAGTC 1 cut(s) 507
Psp6I CCWGG 1 cut(s) 268
PspGI CCWGG 1 cut(s) 268
PspN4I GGNNCC 2 cut(s) 390, 570
PspPI GGNCC 1 cut(s) 425
PsuI RGATCY 1 cut(s) 541
PvuII CAGCTG 1 cut(s) 447
RsaI GTAC 1 cut(s) 412
RsaNI GTAC 1 cut(s) 411
RseI CAYNNNNRTG 1 cut(s) 762
SalI GTCGAC 1 cut(s) 529
SaqAI TTAA 4 cut(s) 176, 356, 641, 824
SatI GCNGC 1 cut(s) 481
Sau3AI GATC 5 cut(s) 541, 577, 628, 646, 664
Sau96I GGNCC 1 cut(s) 425
SchI GAGTC 1 cut(s) 507
ScrFI CCNGG 1 cut(s) 270
SfaNI GCATC 1 cut(s) 699
SfcI CTRYAG 1 cut(s) 442
SmiMI CAYNNNNRTG 1 cut(s) 762
Sse9I AATT 5 cut(s) 331, 376, 415, 744, 825
SsiI CCGC 2 cut(s) 342, 369
SspI AATATT 1 cut(s) 67
SspMI CTAG 1 cut(s) 837
StyD4I CCNGG 1 cut(s) 268
TaiI ACGT 1 cut(s) 423
TaqI TCGA 1 cut(s) 530
TaqII GACCGA 1 cut(s) 726
TasI AATT 5 cut(s) 331, 376, 415, 744, 825
TfiI GAWTC 1 cut(s) 250
Tru1I TTAA 4 cut(s) 176, 356, 641, 824
Tru9I TTAA 4 cut(s) 176, 356, 641, 824
TseI GCWGC 1 cut(s) 480
TspDTI ATGAA 4 cut(s) 17, 206, 242, 303
TspGWI ACGGA 1 cut(s) 194
XagI CCTNNNNNAGG 1 cut(s) 692
XapI RAATTY 1 cut(s) 376
XmiI GTMKAC 1 cut(s) 530
XspI CTAG 1 cut(s) 837
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.