Rh2AG543500

Non-specific serine threonine protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0015671
Physical Location & Seq
Forward (+)
77212126 .. 77217693
5568 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG543500.1

Sequence Viewer

Length: 1011 bp
ATGGAGAACGGAACTCATCTCAGCATGTCCTTAACCCCATTGCCTTCCATTTCCGGCCTCTTCAAGACCGCGGAACCCAAAGACCAAACGCCGATCGTGATGCGGCGGCGCAAATGGCTTGATTTTTTGACTTCATCCTCTGCTCTAGGCAAAAGTCTTCAGCACTATAGAAGAAACATGGCTCCAAAATTGGAAGGTTACGAGTTTGGTGAGACAGTTGCCAGGGTTGCTGGTGCCAAGTTGAAGAAAGCCGTACATATTGCAACTGGACAGACCGTGGCTGTCAAAATATTATCTTTTAAAGGATTTGCAAAAGTTAATCATGGGGCACTACAGAAGTTTAAGAAGAGAAGGCACAGAGAGATGTTTGTGGCAAAGCATTTGAAGCACCCAAATATTATGACTTGCTATATGGGGCACATGTATAAAAGAAGGGCATATATTGTGATGGAATGGATGGAGGGTGGGAGTTTAGATGACTATCTTACACAAAGAGGAGGGACATTAACTGAAGGGGAAGCTCGCAGATACTTCCGCCAGCTCATTCATGACTTTGGAATAAGCAATTATCTGACCGCTGATTTGACATTAAGTTCCTATTGCGGAACAAATTTATATCAGGCTCAAGAGATAAGCAAAGATGGTACAAATTACAGTGGCCCAGAGGTTGATATCTACAGCTGTGGTGTAACACTTTACCACCTCTTATTCGGCTGCCCATCACTACGGAGCTTTCAATATGGCAGACTCTCTATTCCTCTGTCGGCCCCAGCAGATGCAGGAGATCTAATAAGTAGAATGACGGAATGGGATCCAAAGCAGCGTATCACCATCCGAGAAATCAAGAACCATAGATGGTTTCAAGGTGGGGATCAAATGTTCTTAAATGATCCTCATGGCATTTCTGATCAGCGCAGAGGTTGGTGTCCTCAAACAGACCCACCAAGTAAGCCTCCGCCGCCTCCTGAAGCGCCGCCACGGCGCTGCTCTGGAACCGGAGATCAGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001101 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007275 GO:0007568 GO:0007584 GO:0008150 GO:0008152 GO:0009593 GO:0009594 GO:0009605 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009743 GO:0009755 GO:0009756 GO:0009791 GO:0009987 GO:0009991 GO:0010033 GO:0010050 GO:0010150 GO:0010182 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022414 GO:0022622 GO:0023052 GO:0031667 GO:0032501 GO:0032502 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0048364 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051606 GO:0051716 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071322 GO:0071396 GO:0071495 GO:0071704 GO:0080022 GO:0090693 GO:0097305 GO:0097306 GO:0099402 GO:0140096 GO:1901564 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
ko04068 ko04140 ko04150 ko04151 ko04152 ko04211 ko04213 ko04371 ko04530 ko04710 ko04714 ko04910 ko04920 ko04921 ko04922 ko04931 ko04932 ko05410 ko05418 map04068 map04140 map04150 map04151 map04152 map04211 map04213 map04371 map04530 map04710 map04714 map04910 map04920 map04921 map04922 map04931 map04932 map05410 map05418
Pfam Domains
Protein Families
KA1 Pkinase UBA

Protein Analysis

336

Amino Acids

38.0

Weight (kDa)

9.55

Isoelectric Point (pI)

48.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 76 - 180 8.3e-09 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 78 - 182 1.2e-13 Protein kinase domain
Pkinase PF00069 182 - 287 2.5e-16 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 233
AccII CGCG 1 cut(s) 71
AclWI GGATC 4 cut(s) 806, 819, 879, 884
AcsI RAATTY 1 cut(s) 610
AcuI CTGAAG 3 cut(s) 143, 531, 987
AfaI GTAC 2 cut(s) 255, 646
AflIII ACRYGT 1 cut(s) 420
AgsI TTSAA 5 cut(s) 64, 244, 385, 737, 863
AjnI CCWGG 1 cut(s) 221
AloI GAACNNNNNNTCC 2 cut(s) 863, 895
AluBI AGCT 4 cut(s) 521, 541, 681, 732
AluI AGCT 4 cut(s) 521, 541, 681, 732
Alw26I GTCTC 1 cut(s) 206
AlwI GGATC 4 cut(s) 806, 819, 879, 884
AoxI GGCC 3 cut(s) 55, 658, 765
ApeKI GCWGC 3 cut(s) 714, 820, 984
ApoI RAATTY 1 cut(s) 610
ArsI GACNNNNNNTTYG 2 cut(s) 565, 597
AspLEI GCGC 4 cut(s) 111, 915, 973, 984
AspS9I GGNCC 2 cut(s) 659, 766
AsuHPI GGTGA 2 cut(s) 221, 820
BaeGI GKGCMC 2 cut(s) 331, 420
BamHI GGATCC 1 cut(s) 811
BanI GGYRCC 1 cut(s) 233
BbsI GAAGAC 1 cut(s) 149
BbvI GCAGC 3 cut(s) 701, 832, 971
BccI CCATC 6 cut(s) 442, 451, 635, 727, 839, 849
BceAI ACGGC 2 cut(s) 236, 995
BcgI CGANNNNNNTGC 2 cut(s) 82, 116
BciT130I CCWGG 1 cut(s) 223
BclI TGATCA 1 cut(s) 907
BcoDI GTCTC 1 cut(s) 206
BfaI CTAG 1 cut(s) 146
BfmI CTRYAG 3 cut(s) 166, 332, 676
BfoI RGCGCY 2 cut(s) 974, 985
BglI GCCNNNNNGGC 1 cut(s) 979
BglII AGATCT 1 cut(s) 784
BisI GCNGC 7 cut(s) 104, 107, 715, 821, 959, 974, 985
BlsI GCNGC 7 cut(s) 105, 108, 716, 822, 960, 975, 986
Bme1390I CCNGG 1 cut(s) 223
BmgT120I GGNCC 2 cut(s) 659, 766
BmiI GGNNCC 6 cut(s) 75, 183, 235, 768, 813, 994
BmrFI CCNGG 1 cut(s) 223
BmsI GCATC 2 cut(s) 90, 766
BpiI GAAGAC 1 cut(s) 149
BpuEI CTTGAG 1 cut(s) 609
BsaBI GATNNNNATC 1 cut(s) 480
BsaJI CCNNGG 4 cut(s) 69, 222, 276, 977
BsaWI WCCGGW 1 cut(s) 995
Bse1I ACTGG 1 cut(s) 271
Bse3DI GCAATG 1 cut(s) 38
Bse8I GATNNNNATC 1 cut(s) 480
BseBI CCWGG 1 cut(s) 223
BseDI CCNNGG 4 cut(s) 69, 222, 276, 977
BseGI GGATG 3 cut(s) 134, 462, 831
BseJI GATNNNNATC 1 cut(s) 480
BseMI GCAATG 1 cut(s) 38
BseMII CTCAG 1 cut(s) 34
BseNI ACTGG 1 cut(s) 271
BseRI GAGGAG 1 cut(s) 510
BseSI GKGCMC 2 cut(s) 331, 420
BseXI GCAGC 3 cut(s) 701, 832, 971
BseYI CCCAGC 1 cut(s) 769
Bsh1236I CGCG 1 cut(s) 71
Bsh1285I CGRYCG 1 cut(s) 96
BshFI GGCC 3 cut(s) 57, 660, 767
BshNI GGYRCC 1 cut(s) 233
BsiEI CGRYCG 1 cut(s) 96
BsiSI CCGG 2 cut(s) 54, 996
BslFI GGGAC 1 cut(s) 514
BsmAI GTCTC 1 cut(s) 206
BsmFI GGGAC 1 cut(s) 514
BsnI GGCC 3 cut(s) 57, 660, 767
Bsp1286I GDGCHC 2 cut(s) 331, 420
Bsp143I GATC 7 cut(s) 93, 784, 811, 871, 889, 907, 1000
BspANI GGCC 3 cut(s) 57, 660, 767
BspCNI CTCAG 1 cut(s) 33
BspFNI CGCG 1 cut(s) 71
BspHI TCATGA 1 cut(s) 547
BspLI GGNNCC 6 cut(s) 75, 183, 235, 768, 813, 994
BspPI GGATC 4 cut(s) 806, 819, 879, 884
BspT107I GGYRCC 1 cut(s) 233
BsrDI GCAATG 1 cut(s) 38
BsrI ACTGG 1 cut(s) 271
BssECI CCNNGG 4 cut(s) 69, 222, 276, 977
BssMI GATC 7 cut(s) 93, 784, 811, 871, 889, 907, 1000
Bst2UI CCWGG 1 cut(s) 223
Bst4CI ACNGT 3 cut(s) 217, 277, 656
Bst6I CTCTTC 2 cut(s) 65, 341
BstC8I GCNNGC 2 cut(s) 523, 539
BstDEI CTNAG 1 cut(s) 20
BstDSI CCRYGG 3 cut(s) 69, 276, 977
BstF5I GGATG 3 cut(s) 134, 462, 831
BstFNI CGCG 1 cut(s) 71
BstH2I RGCGCY 2 cut(s) 974, 985
BstHHI GCGC 4 cut(s) 111, 915, 973, 984
BstKTI GATC 7 cut(s) 96, 787, 814, 874, 892, 910, 1003
BstMAI GTCTC 1 cut(s) 206
BstMBI GATC 7 cut(s) 93, 784, 811, 871, 889, 907, 1000
BstMCI CGRYCG 1 cut(s) 96
BstMWI GCNNNNNNNGC 5 cut(s) 115, 227, 385, 958, 979
BstNI CCWGG 1 cut(s) 223
BstNSI RCATGY 2 cut(s) 28, 424
BstSCI CCNGG 1 cut(s) 221
BstSFI CTRYAG 3 cut(s) 166, 332, 676
BstSLI GKGCMC 2 cut(s) 331, 420
BstUI CGCG 1 cut(s) 71
BstV1I GCAGC 3 cut(s) 701, 832, 971
BstV2I GAAGAC 1 cut(s) 149
BstX2I RGATCY 2 cut(s) 784, 811
BstYI RGATCY 2 cut(s) 784, 811
BsuRI GGCC 3 cut(s) 57, 660, 767
BtgI CCRYGG 3 cut(s) 69, 276, 977
BtsCI GGATG 3 cut(s) 134, 462, 831
BtsIMutI CAGTG 1 cut(s) 661
Cac8I GCNNGC 2 cut(s) 523, 539
CciI TCATGA 1 cut(s) 547
CfoI GCGC 4 cut(s) 111, 915, 973, 984
Cfr13I GGNCC 2 cut(s) 659, 766
Cfr42I CCGCGG 1 cut(s) 72
Csp6I GTAC 2 cut(s) 254, 645
CviAII CATG 6 cut(s) 25, 178, 323, 421, 548, 896
CviQI GTAC 2 cut(s) 254, 645
DdeI CTNAG 1 cut(s) 20
DpnI GATC 7 cut(s) 95, 786, 813, 873, 891, 909, 1002
DpnII GATC 7 cut(s) 93, 784, 811, 871, 889, 907, 1000
DraI TTTAAA 1 cut(s) 301
Eam1104I CTCTTC 2 cut(s) 65, 341
EarI CTCTTC 2 cut(s) 65, 341
EciI GGCGGA 2 cut(s) 524, 945
Eco32I GATATC 1 cut(s) 673
Eco57I CTGAAG 3 cut(s) 143, 531, 987
EcoRII CCWGG 1 cut(s) 221
EcoRV GATATC 1 cut(s) 673
FaeI CATG 6 cut(s) 28, 181, 326, 424, 551, 899
FaqI GGGAC 1 cut(s) 514
FatI CATG 6 cut(s) 24, 177, 322, 420, 547, 895
FbaI TGATCA 1 cut(s) 907
Fnu4HI GCNGC 7 cut(s) 104, 107, 715, 821, 959, 974, 985
FokI GGATG 3 cut(s) 121, 469, 818
Fsp4HI GCNGC 7 cut(s) 104, 107, 715, 821, 959, 974, 985
FspBI CTAG 1 cut(s) 146
GlaI GCGC 4 cut(s) 110, 914, 972, 983
GluI GCNGC 7 cut(s) 104, 107, 715, 821, 959, 974, 985
GsaI CCCAGC 1 cut(s) 773
HaeII RGCGCY 2 cut(s) 974, 985
HaeIII GGCC 3 cut(s) 57, 660, 767
HapII CCGG 2 cut(s) 54, 996
HhaI GCGC 4 cut(s) 111, 915, 973, 984
Hin1II CATG 6 cut(s) 28, 181, 326, 424, 551, 899
Hin6I GCGC 4 cut(s) 109, 913, 971, 982
HinP1I GCGC 4 cut(s) 109, 913, 971, 982
HinfI GANTC 1 cut(s) 747
HpaII CCGG 2 cut(s) 54, 996
HphI GGTGA 2 cut(s) 221, 820
Hpy188I TCNGA 3 cut(s) 573, 836, 907
Hpy188III TCNNGA 8 cut(s) 64, 97, 548, 626, 844, 965, 990, 1008
HpyAV CCTTC 5 cut(s) 54, 188, 345, 426, 506
HpyCH4III ACNGT 3 cut(s) 217, 277, 656
HpyCH4V TGCA 3 cut(s) 263, 311, 779
HpyF10VI GCNNNNNNNGC 5 cut(s) 115, 227, 385, 958, 979
HpyF3I CTNAG 1 cut(s) 20
Hsp92II CATG 6 cut(s) 28, 181, 326, 424, 551, 899
HspAI GCGC 4 cut(s) 109, 913, 971, 982
Ksp22I TGATCA 1 cut(s) 907
KspI CCGCGG 1 cut(s) 72
Kzo9I GATC 7 cut(s) 93, 784, 811, 871, 889, 907, 1000
LmnI GCTCC 2 cut(s) 187, 729
Lsp1109I GCAGC 3 cut(s) 701, 832, 971
LweI GCATC 2 cut(s) 90, 766
MaeI CTAG 1 cut(s) 146
MaeIII GTNAC 2 cut(s) 197, 688
MalI GATC 7 cut(s) 95, 786, 813, 873, 891, 909, 1002
MboI GATC 7 cut(s) 93, 784, 811, 871, 889, 907, 1000
MboII GAAGA 5 cut(s) 52, 149, 183, 256, 358
MflI RGATCY 2 cut(s) 784, 811
MhlI GDGCHC 2 cut(s) 331, 420
MluCI AATT 4 cut(s) 188, 565, 610, 649
MlyI GAGTC 1 cut(s) 741
MseI TTAA 7 cut(s) 32, 300, 318, 342, 506, 590, 884
MspA1I CMGCKG 3 cut(s) 71, 578, 681
MspI CCGG 2 cut(s) 54, 996
MspR9I CCNGG 1 cut(s) 223
MvaI CCWGG 1 cut(s) 223
MvnI CGCG 1 cut(s) 71
MwoI GCNNNNNNNGC 5 cut(s) 115, 227, 385, 958, 979
NdeII GATC 7 cut(s) 93, 784, 811, 871, 889, 907, 1000
NlaIII CATG 6 cut(s) 28, 181, 326, 424, 551, 899
NlaIV GGNNCC 6 cut(s) 75, 183, 235, 768, 813, 994
NspI RCATGY 2 cut(s) 28, 424
PagI TCATGA 1 cut(s) 547
PciI ACATGT 1 cut(s) 420
PkrI GCNGC 7 cut(s) 105, 108, 716, 822, 960, 975, 986
Ple19I CGATCG 1 cut(s) 96
PleI GAGTC 1 cut(s) 741
PpsI GAGTC 1 cut(s) 741
PscI ACATGT 1 cut(s) 420
Psp6I CCWGG 1 cut(s) 221
PspFI CCCAGC 1 cut(s) 769
PspGI CCWGG 1 cut(s) 221
PspN4I GGNNCC 6 cut(s) 75, 183, 235, 768, 813, 994
PspPI GGNCC 2 cut(s) 659, 766
PsuI RGATCY 2 cut(s) 784, 811
PvuI CGATCG 1 cut(s) 96
PvuII CAGCTG 1 cut(s) 681
RsaI GTAC 2 cut(s) 255, 646
RsaNI GTAC 2 cut(s) 254, 645
SacII CCGCGG 1 cut(s) 72
SaqAI TTAA 7 cut(s) 32, 300, 318, 342, 506, 590, 884
SatI GCNGC 7 cut(s) 104, 107, 715, 821, 959, 974, 985
Sau3AI GATC 7 cut(s) 93, 784, 811, 871, 889, 907, 1000
Sau96I GGNCC 2 cut(s) 659, 766
SchI GAGTC 1 cut(s) 741
ScrFI CCNGG 1 cut(s) 223
SduI GDGCHC 2 cut(s) 331, 420
SetI ASST 9 cut(s) 199, 523, 543, 669, 683, 705, 734, 868, 922
SfaNI GCATC 2 cut(s) 90, 766
SfcI CTRYAG 3 cut(s) 166, 332, 676
Sfr303I CCGCGG 1 cut(s) 72
SgrBI CCGCGG 1 cut(s) 72
SmlI CTYRAG 1 cut(s) 624
SmoI CTYRAG 1 cut(s) 624
Sse9I AATT 4 cut(s) 188, 565, 610, 649
SspI AATATT 2 cut(s) 291, 397
SspMI CTAG 1 cut(s) 146
StyD4I CCNGG 1 cut(s) 221
TaaI ACNGT 3 cut(s) 217, 277, 656
TasI AATT 4 cut(s) 188, 565, 610, 649
TauI GCSGC 4 cut(s) 106, 109, 961, 976
Tru1I TTAA 7 cut(s) 32, 300, 318, 342, 506, 590, 884
Tru9I TTAA 7 cut(s) 32, 300, 318, 342, 506, 590, 884
TscAI CASTG 1 cut(s) 661
TseI GCWGC 3 cut(s) 714, 820, 984
TspDTI ATGAA 2 cut(s) 123, 536
TspGWI ACGGA 3 cut(s) 24, 742, 818
TspRI CASTG 1 cut(s) 661
XapI RAATTY 1 cut(s) 610
XceI RCATGY 2 cut(s) 28, 424
XspI CTAG 1 cut(s) 146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.