FvH4_6g49320

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0014779
Physical Location & Seq
Reverse (-)
36979788 .. 36980309
522 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g49320.t1

Sequence Viewer

Length: 522 bp
ATGACCAAGACAAAACCAACCCTCCCAACCAGCACCAACAACTGCCACCCTTCTCCTACACATGTTGCTGCTCAATTCACTCTTAGCTTGCCGTCCGTTGTGTTCTTTAACCTCACAATAACAACCCTCACAACCATGTACCGCGCCTACCAGCACAGAGACATCACCTTGCTCGCTTTCCTCGCATTCGTCTACGCATCCTATTTCATCCTCCATAACTGTGTAGAGGTCTACAGCCGACTTCCCAAGGGCGAAAAGTCGACCAAGAAAGAGCTGCTGAGGTTCACTTTGTGGTTTTTGCCAACAGTAGTGTTTTTTGGATTTGCTCTTGAGTTTGGGACGTTCATGCCCGTGACTGTGGTTGTGCTAATGTATTGTCTCGCCATTGCTAGCAGCTCGTTGCTGTTCTATGCTCACTATATTCAGGATGCTCAAAAGGGATATAATGCTTGCGCTGATGATCAGAAGAAGAGTAGCAGCAACAAGCATGATCATGTACCAAGTGCCCAAAACATTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

174

Amino Acids

19.55

Weight (kDa)

9.05

Isoelectric Point (pI)

47.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6490 PF20100 38 - 142 1.2e-07 Family of unknown function (DUF6490)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 192, 231, 260
AccII CGCG 1 cut(s) 144
AciI CCGC 1 cut(s) 142
AdeI CACNNNGTG 1 cut(s) 291
AfaI GTAC 2 cut(s) 140, 498
AflIII ACRYGT 1 cut(s) 61
AluBI AGCT 3 cut(s) 87, 274, 396
AluI AGCT 3 cut(s) 87, 274, 396
Alw26I GTCTC 2 cut(s) 153, 383
ApeKI GCWGC 4 cut(s) 68, 274, 393, 477
AspLEI GCGC 2 cut(s) 146, 455
AsuHPI GGTGA 1 cut(s) 157
AsuNHI GCTAGC 1 cut(s) 389
BaeGI GKGCMC 1 cut(s) 508
BbvCI CCTCAGC 1 cut(s) 278
BbvI GCAGC 4 cut(s) 55, 261, 405, 489
BceAI ACGGC 1 cut(s) 76
BclI TGATCA 2 cut(s) 460, 490
BcoDI GTCTC 2 cut(s) 153, 383
BfaI CTAG 1 cut(s) 390
BfmI CTRYAG 1 cut(s) 232
BisI GCNGC 4 cut(s) 69, 275, 394, 478
BlsI GCNGC 4 cut(s) 70, 276, 395, 479
BmsI GCATC 2 cut(s) 206, 418
BmtI GCTAGC 1 cut(s) 393
Bpu10I CCTNAGC 1 cut(s) 278
BpuEI CTTGAG 1 cut(s) 350
BsaJI CCNNGG 1 cut(s) 246
BsaXI ACNNNNNCTCC 2 cut(s) 6, 36
Bse3DI GCAATG 1 cut(s) 384
BseDI CCNNGG 1 cut(s) 246
BseGI GGATG 3 cut(s) 197, 207, 433
BseMI GCAATG 1 cut(s) 384
BseMII CTCAG 1 cut(s) 269
BseSI GKGCMC 1 cut(s) 508
BseXI GCAGC 4 cut(s) 55, 261, 405, 489
Bsh1236I CGCG 1 cut(s) 144
BslFI GGGAC 1 cut(s) 352
BsmAI GTCTC 2 cut(s) 153, 383
BsmFI GGGAC 1 cut(s) 352
BsmI GAATGC 1 cut(s) 185
Bsp1286I GDGCHC 1 cut(s) 508
Bsp143I GATC 2 cut(s) 460, 490
BspACI CCGC 1 cut(s) 142
BspCNI CTCAG 1 cut(s) 270
BspFNI CGCG 1 cut(s) 144
BspOI GCTAGC 1 cut(s) 393
BsrDI GCAATG 1 cut(s) 384
BssECI CCNNGG 1 cut(s) 246
BssMI GATC 2 cut(s) 460, 490
BssT1I CCWWGG 1 cut(s) 246
Bst4CI ACNGT 3 cut(s) 221, 307, 358
Bst6I CTCTTC 1 cut(s) 464
BstC8I GCNNGC 4 cut(s) 89, 174, 391, 451
BstDEI CTNAG 2 cut(s) 83, 278
BstF5I GGATG 3 cut(s) 197, 207, 433
BstFNI CGCG 1 cut(s) 144
BstHHI GCGC 2 cut(s) 146, 455
BstKTI GATC 2 cut(s) 463, 493
BstMAI GTCTC 2 cut(s) 153, 383
BstMBI GATC 2 cut(s) 460, 490
BstMWI GCNNNNNNNGC 1 cut(s) 182
BstNSI RCATGY 1 cut(s) 65
BstSFI CTRYAG 1 cut(s) 232
BstSLI GKGCMC 1 cut(s) 508
BstUI CGCG 1 cut(s) 144
BstV1I GCAGC 4 cut(s) 55, 261, 405, 489
BtsCI GGATG 3 cut(s) 197, 207, 433
Cac8I GCNNGC 4 cut(s) 89, 174, 391, 451
CfoI GCGC 2 cut(s) 146, 455
Csp6I GTAC 2 cut(s) 139, 497
CviAII CATG 5 cut(s) 62, 136, 346, 488, 494
CviJI RGCY 4 cut(s) 87, 237, 274, 396
CviKI_1 RGCY 4 cut(s) 87, 237, 274, 396
CviQI GTAC 2 cut(s) 139, 497
DdeI CTNAG 2 cut(s) 83, 278
DpnI GATC 2 cut(s) 462, 492
DpnII GATC 2 cut(s) 460, 490
DraIII CACNNNGTG 1 cut(s) 291
Eam1104I CTCTTC 1 cut(s) 464
EarI CTCTTC 1 cut(s) 464
Eco130I CCWWGG 1 cut(s) 246
EcoT14I CCWWGG 1 cut(s) 246
ErhI CCWWGG 1 cut(s) 246
FaeI CATG 5 cut(s) 65, 139, 349, 491, 497
FaiI YATR 9 cut(s) 63, 137, 216, 347, 411, 420, 444, 489, 495
FaqI GGGAC 1 cut(s) 352
FatI CATG 5 cut(s) 61, 135, 345, 487, 493
FbaI TGATCA 2 cut(s) 460, 490
FblI GTMKAC 3 cut(s) 192, 231, 260
Fnu4HI GCNGC 4 cut(s) 69, 275, 394, 478
FokI GGATG 3 cut(s) 184, 194, 440
Fsp4HI GCNGC 4 cut(s) 69, 275, 394, 478
FspBI CTAG 1 cut(s) 390
GlaI GCGC 2 cut(s) 145, 454
GluI GCNGC 4 cut(s) 69, 275, 394, 478
HhaI GCGC 2 cut(s) 146, 455
Hin1II CATG 5 cut(s) 65, 139, 349, 491, 497
Hin6I GCGC 2 cut(s) 144, 453
HinP1I GCGC 2 cut(s) 144, 453
HincII GTYRAC 1 cut(s) 261
HindII GTYRAC 1 cut(s) 261
HphI GGTGA 1 cut(s) 157
Hpy166II GTNNAC 4 cut(s) 193, 232, 261, 285
Hpy188I TCNGA 1 cut(s) 465
Hpy188III TCNNGA 2 cut(s) 329, 425
Hpy8I GTNNAC 4 cut(s) 193, 232, 261, 285
HpyAV CCTTC 1 cut(s) 60
HpyCH4III ACNGT 3 cut(s) 221, 307, 358
HpyCH4IV ACGT 1 cut(s) 341
HpyF10VI GCNNNNNNNGC 1 cut(s) 182
HpyF3I CTNAG 2 cut(s) 83, 278
HpySE526I ACGT 1 cut(s) 341
Hsp92II CATG 5 cut(s) 65, 139, 349, 491, 497
HspAI GCGC 2 cut(s) 144, 453
Ksp22I TGATCA 2 cut(s) 460, 490
Kzo9I GATC 2 cut(s) 460, 490
LpnPI CCDG 3 cut(s) 43, 164, 410
Lsp1109I GCAGC 4 cut(s) 55, 261, 405, 489
LweI GCATC 2 cut(s) 206, 418
MaeI CTAG 1 cut(s) 390
MaeII ACGT 1 cut(s) 341
MaeIII GTNAC 1 cut(s) 352
MalI GATC 2 cut(s) 462, 492
MboI GATC 2 cut(s) 460, 490
MboII GAAGA 2 cut(s) 478, 481
MhlI GDGCHC 1 cut(s) 508
MluCI AATT 1 cut(s) 74
MnlI CCTC 7 cut(s) 32, 122, 137, 191, 220, 221, 273
MseI TTAA 1 cut(s) 108
MslI CAYNNNNRTG 4 cut(s) 134, 219, 350, 492
Mva1269I GAATGC 1 cut(s) 185
MvnI CGCG 1 cut(s) 144
MwoI GCNNNNNNNGC 1 cut(s) 182
NdeII GATC 2 cut(s) 460, 490
NheI GCTAGC 1 cut(s) 389
NlaIII CATG 5 cut(s) 65, 139, 349, 491, 497
NmuCI GTSAC 1 cut(s) 352
NspI RCATGY 1 cut(s) 65
PciI ACATGT 1 cut(s) 61
PctI GAATGC 1 cut(s) 185
PkrI GCNGC 4 cut(s) 70, 276, 395, 479
PscI ACATGT 1 cut(s) 61
RsaI GTAC 2 cut(s) 140, 498
RsaNI GTAC 2 cut(s) 139, 497
RseI CAYNNNNRTG 4 cut(s) 134, 219, 350, 492
SalI GTCGAC 1 cut(s) 259
SaqAI TTAA 1 cut(s) 108
SatI GCNGC 4 cut(s) 69, 275, 394, 478
Sau3AI GATC 2 cut(s) 460, 490
SduI GDGCHC 1 cut(s) 508
SetI ASST 8 cut(s) 89, 114, 170, 231, 276, 284, 344, 398
SfaNI GCATC 2 cut(s) 206, 418
SfcI CTRYAG 1 cut(s) 232
SmiMI CAYNNNNRTG 4 cut(s) 134, 219, 350, 492
SmlI CTYRAG 1 cut(s) 329
SmoI CTYRAG 1 cut(s) 329
Sse9I AATT 1 cut(s) 74
SsiI CCGC 1 cut(s) 142
SspMI CTAG 1 cut(s) 390
StyI CCWWGG 1 cut(s) 246
TaaI ACNGT 3 cut(s) 221, 307, 358
TaiI ACGT 1 cut(s) 344
TaqI TCGA 1 cut(s) 260
TasI AATT 1 cut(s) 74
Tru1I TTAA 1 cut(s) 108
Tru9I TTAA 1 cut(s) 108
TseFI GTSAC 1 cut(s) 352
TseI GCWGC 4 cut(s) 68, 274, 393, 477
Tsp45I GTSAC 1 cut(s) 352
TspDTI ATGAA 2 cut(s) 196, 334
TspGWI ACGGA 1 cut(s) 85
XceI RCATGY 1 cut(s) 65
XmiI GTMKAC 3 cut(s) 192, 231, 260
XspI CTAG 1 cut(s) 390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.