Rmu_co8400523.1_g000001

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8400523.1
OG0014779
Physical Location & Seq
Reverse (-)
306 .. 812
507 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8400523.1_g000001.1.cds

Sequence Viewer

Length: 507 bp
atgccaaacacaaaaccagctctccctaccagcaacaccaatcgccaccctcctacagatactgctcaatttactattagcttgccgtccgttgtgttcataaacctcacagtaacaaccttcacaaccatgtaccgcgcgcagcagcaccacgacaccaccttgctcgctttccttgcattcgtctacgcgtcctatttcatcctccataactgtgtagaagtttacagtcgactgcccaagggcgaaaagtcgacgaagaaagagctgctgaggttcactctgtggtttctgccaactgcagtgctgttgggatttgcagctgagtttgggactttcatgaacgtgactgtggttgtgctcatgtactgtctcgccattgctagcagcttgttgctgttctatgtccactacattcatgacgatcagaagggacatgcttgtcctattcagaagatcagtaacaacaagcatgatcatgtaccaattgcccaaaacattgtctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

168

Amino Acids

19.0

Weight (kDa)

8.79

Isoelectric Point (pI)

40.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 441
AccI GTMKAC 3 cut(s) 186, 232, 254
AccII CGCG 3 cut(s) 138, 140, 191
AciI CCGC 1 cut(s) 136
AdeI CACNNNGTG 1 cut(s) 285
AfaI GTAC 3 cut(s) 134, 368, 483
AflIII ACRYGT 1 cut(s) 189
AluBI AGCT 5 cut(s) 20, 81, 268, 323, 390
AluI AGCT 5 cut(s) 20, 81, 268, 323, 390
Alw21I GWGCWC 1 cut(s) 363
Alw26I GTCTC 1 cut(s) 377
AlwNI CAGNNNCTG 1 cut(s) 62
ApeKI GCWGC 5 cut(s) 142, 145, 268, 320, 387
AspLEI GCGC 2 cut(s) 140, 142
AsuNHI GCTAGC 1 cut(s) 383
Bbv12I GWGCWC 1 cut(s) 363
BbvCI CCTCAGC 1 cut(s) 272
BbvI GCAGC 5 cut(s) 154, 157, 255, 332, 399
BceAI ACGGC 1 cut(s) 70
BclI TGATCA 1 cut(s) 475
BcoDI GTCTC 1 cut(s) 377
BfaI CTAG 1 cut(s) 384
BfmI CTRYAG 2 cut(s) 54, 300
BisI GCNGC 5 cut(s) 143, 146, 269, 321, 388
BlsI GCNGC 5 cut(s) 144, 147, 270, 322, 389
BmtI GCTAGC 1 cut(s) 387
BplI GAGNNNNNCTC 2 cut(s) 265, 297
Bpu10I CCTNAGC 1 cut(s) 272
BsaJI CCNNGG 1 cut(s) 240
BsaXI ACNNNNNCTCC 2 cut(s) 6, 36
Bse3DI GCAATG 1 cut(s) 378
BseDI CCNNGG 1 cut(s) 240
BseGI GGATG 1 cut(s) 201
BseMI GCAATG 1 cut(s) 378
BseMII CTCAG 2 cut(s) 263, 315
BsePI GCGCGC 1 cut(s) 138
BseXI GCAGC 5 cut(s) 154, 157, 255, 332, 399
Bsh1236I CGCG 3 cut(s) 138, 140, 191
BsiHKAI GWGCWC 1 cut(s) 363
BslFI GGGAC 2 cut(s) 346, 447
BsmAI GTCTC 1 cut(s) 377
BsmFI GGGAC 2 cut(s) 346, 447
BsmI GAATGC 1 cut(s) 179
Bsp1286I GDGCHC 1 cut(s) 363
Bsp143I GATC 3 cut(s) 424, 456, 475
BspACI CCGC 1 cut(s) 136
BspCNI CTCAG 2 cut(s) 264, 316
BspFNI CGCG 3 cut(s) 138, 140, 191
BspHI TCATGA 2 cut(s) 339, 418
BspMAI CTGCAG 1 cut(s) 304
BspOI GCTAGC 1 cut(s) 387
BsrDI GCAATG 1 cut(s) 378
BssECI CCNNGG 1 cut(s) 240
BssHII GCGCGC 1 cut(s) 138
BssMI GATC 3 cut(s) 424, 456, 475
BssT1I CCWWGG 1 cut(s) 240
Bst4CI ACNGT 5 cut(s) 112, 215, 230, 352, 371
BstC8I GCNNGC 4 cut(s) 83, 140, 168, 385
BstDEI CTNAG 2 cut(s) 272, 324
BstF5I GGATG 1 cut(s) 201
BstFNI CGCG 3 cut(s) 138, 140, 191
BstHHI GCGC 2 cut(s) 140, 142
BstKTI GATC 3 cut(s) 427, 459, 478
BstMAI GTCTC 1 cut(s) 377
BstMBI GATC 3 cut(s) 424, 456, 475
BstMWI GCNNNNNNNGC 1 cut(s) 176
BstNSI RCATGY 1 cut(s) 440
BstSFI CTRYAG 2 cut(s) 54, 300
BstUI CGCG 3 cut(s) 138, 140, 191
BstV1I GCAGC 5 cut(s) 154, 157, 255, 332, 399
BtsCI GGATG 1 cut(s) 201
BtsI GCAGTG 1 cut(s) 309
BtsIMutI CAGTG 1 cut(s) 309
Cac8I GCNNGC 4 cut(s) 83, 140, 168, 385
CaiI CAGNNNCTG 1 cut(s) 62
CciI TCATGA 2 cut(s) 339, 418
CfoI GCGC 2 cut(s) 140, 142
CseI GACGC 1 cut(s) 180
Csp6I GTAC 3 cut(s) 133, 367, 482
CviAII CATG 7 cut(s) 130, 340, 364, 419, 437, 473, 479
CviJI RGCY 5 cut(s) 20, 81, 268, 323, 390
CviKI_1 RGCY 5 cut(s) 20, 81, 268, 323, 390
CviQI GTAC 3 cut(s) 133, 367, 482
DdeI CTNAG 2 cut(s) 272, 324
DpnI GATC 3 cut(s) 426, 458, 477
DpnII GATC 3 cut(s) 424, 456, 475
DraIII CACNNNGTG 1 cut(s) 285
DrdI GACNNNNNNGTC 1 cut(s) 441
DseDI GACNNNNNNGTC 1 cut(s) 441
Eco130I CCWWGG 1 cut(s) 240
EcoT14I CCWWGG 1 cut(s) 240
ErhI CCWWGG 1 cut(s) 240
FaeI CATG 7 cut(s) 133, 343, 367, 422, 440, 476, 482
FaqI GGGAC 2 cut(s) 346, 447
FatI CATG 7 cut(s) 129, 339, 363, 418, 436, 472, 478
FbaI TGATCA 1 cut(s) 475
FblI GTMKAC 3 cut(s) 186, 232, 254
Fnu4HI GCNGC 5 cut(s) 143, 146, 269, 321, 388
FokI GGATG 1 cut(s) 188
Fsp4HI GCNGC 5 cut(s) 143, 146, 269, 321, 388
FspBI CTAG 1 cut(s) 384
GlaI GCGC 2 cut(s) 139, 141
GluI GCNGC 5 cut(s) 143, 146, 269, 321, 388
HgaI GACGC 1 cut(s) 180
HhaI GCGC 2 cut(s) 140, 142
Hin1II CATG 7 cut(s) 133, 343, 367, 422, 440, 476, 482
Hin6I GCGC 2 cut(s) 138, 140
HinP1I GCGC 2 cut(s) 138, 140
HincII GTYRAC 2 cut(s) 233, 255
HindII GTYRAC 2 cut(s) 233, 255
Hpy166II GTNNAC 6 cut(s) 187, 226, 233, 255, 279, 409
Hpy188I TCNGA 2 cut(s) 429, 453
Hpy188III TCNNGA 2 cut(s) 340, 419
Hpy8I GTNNAC 6 cut(s) 187, 226, 233, 255, 279, 409
Hpy99I CGWCG 1 cut(s) 259
HpyAV CCTTC 2 cut(s) 130, 424
HpyCH4III ACNGT 5 cut(s) 112, 215, 230, 352, 371
HpyCH4IV ACGT 1 cut(s) 345
HpyCH4V TGCA 3 cut(s) 179, 302, 320
HpyF10VI GCNNNNNNNGC 1 cut(s) 176
HpyF3I CTNAG 2 cut(s) 272, 324
HpySE526I ACGT 1 cut(s) 345
Hsp92II CATG 7 cut(s) 133, 343, 367, 422, 440, 476, 482
HspAI GCGC 2 cut(s) 138, 140
Ksp22I TGATCA 1 cut(s) 475
Kzo9I GATC 3 cut(s) 424, 456, 475
LpnPI CCDG 2 cut(s) 30, 43
Lsp1109I GCAGC 5 cut(s) 154, 157, 255, 332, 399
MaeI CTAG 1 cut(s) 384
MaeII ACGT 1 cut(s) 345
MaeIII GTNAC 3 cut(s) 112, 346, 461
MalI GATC 3 cut(s) 426, 458, 477
MboI GATC 3 cut(s) 424, 456, 475
MboII GAAGA 2 cut(s) 271, 466
MfeI CAATTG 1 cut(s) 486
MhlI GDGCHC 1 cut(s) 363
MluCI AATT 2 cut(s) 68, 486
MluI ACGCGT 1 cut(s) 189
MnlI CCTC 4 cut(s) 60, 116, 215, 267
MslI CAYNNNNRTG 4 cut(s) 128, 213, 344, 477
MspA1I CMGCKG 1 cut(s) 323
MunI CAATTG 1 cut(s) 486
Mva1269I GAATGC 1 cut(s) 179
MvnI CGCG 3 cut(s) 138, 140, 191
MwoI GCNNNNNNNGC 1 cut(s) 176
NdeII GATC 3 cut(s) 424, 456, 475
NheI GCTAGC 1 cut(s) 383
NlaIII CATG 7 cut(s) 133, 343, 367, 422, 440, 476, 482
NmuCI GTSAC 1 cut(s) 346
NspI RCATGY 1 cut(s) 440
PagI TCATGA 2 cut(s) 339, 418
PauI GCGCGC 1 cut(s) 138
PctI GAATGC 1 cut(s) 179
PkrI GCNGC 5 cut(s) 144, 147, 270, 322, 389
PstI CTGCAG 1 cut(s) 304
PstNI CAGNNNCTG 1 cut(s) 62
PteI GCGCGC 1 cut(s) 138
PvuII CAGCTG 1 cut(s) 323
RsaI GTAC 3 cut(s) 134, 368, 483
RsaNI GTAC 3 cut(s) 133, 367, 482
RseI CAYNNNNRTG 4 cut(s) 128, 213, 344, 477
SalI GTCGAC 2 cut(s) 231, 253
SatI GCNGC 5 cut(s) 143, 146, 269, 321, 388
Sau3AI GATC 3 cut(s) 424, 456, 475
SduI GDGCHC 1 cut(s) 363
SfcI CTRYAG 2 cut(s) 54, 300
SmiMI CAYNNNNRTG 4 cut(s) 128, 213, 344, 477
Sse9I AATT 2 cut(s) 68, 486
SsiI CCGC 1 cut(s) 136
SspMI CTAG 1 cut(s) 384
StyI CCWWGG 1 cut(s) 240
TaaI ACNGT 5 cut(s) 112, 215, 230, 352, 371
TaiI ACGT 1 cut(s) 348
TaqI TCGA 2 cut(s) 232, 254
TasI AATT 2 cut(s) 68, 486
TatI WGTACW 1 cut(s) 366
TscAI CASTG 1 cut(s) 309
TseFI GTSAC 1 cut(s) 346
TseI GCWGC 5 cut(s) 142, 145, 268, 320, 387
Tsp45I GTSAC 1 cut(s) 346
TspDTI ATGAA 5 cut(s) 88, 190, 328, 356, 407
TspGWI ACGGA 1 cut(s) 79
TspRI CASTG 1 cut(s) 309
XceI RCATGY 1 cut(s) 440
XmiI GTMKAC 3 cut(s) 186, 232, 254
XspI CTAG 1 cut(s) 384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.