RchiOBHm_Chr2g0169171

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0014779
Physical Location & Seq
Forward (+)
83427287 .. 83428159
873 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ53680

Sequence Viewer

Length: 498 bp
ATGGCAGACACTAAAACAGCCCTCCCTACCAGCAACACCAGTTGCCACCCTCATCCTACAGATAGTGTTCAATTTACTATTAGCTTGCCGTCCATTGTGTTCATTAACCTCACAATAACAACCTTCACAACCATGTACCGCGCGCAGCAGCACCACGACACCACCTTGCTAGCTTTCCTCGCATTCGTCTACGCCTCTTATTTCATCCTCCATAACTGTGTGCAAGTGTACAGTCGACTTCCCAATGGCGAAAAATCGGCGAAGAAAGAGCTGCTGAGGTTCACCCTGTGGTTTTTGCCAACACTAGTCCTGCTGGGATTTGCAGCTGAGTTTGGGACTTTCATGAACGTGACTGTGGTTGTGCTTATGTACTGTCTCGCCATTGCTAGCAGCTTGACGCTGTTCTATGTCCACTACATTTATGATGATCAAAAGGGACATGATCAGATGAGTAACAGCAAACATGATCATGTACCAATTGCCCAGAACATTGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

165

Amino Acids

18.68

Weight (kDa)

6.74

Isoelectric Point (pI)

39.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6490 PF20100 37 - 140 7.9e-08 Family of unknown function (DUF6490)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 189, 235
AccII CGCG 2 cut(s) 141, 143
AciI CCGC 1 cut(s) 139
AdeI CACNNNGTG 1 cut(s) 288
AfaI GTAC 4 cut(s) 137, 230, 371, 474
AgsI TTSAA 1 cut(s) 71
AhlI ACTAGT 1 cut(s) 304
AluBI AGCT 5 cut(s) 84, 173, 271, 326, 393
AluI AGCT 5 cut(s) 84, 173, 271, 326, 393
Alw26I GTCTC 1 cut(s) 380
ApeKI GCWGC 5 cut(s) 145, 148, 271, 323, 390
AspLEI GCGC 2 cut(s) 143, 145
AsuHPI GGTGA 1 cut(s) 274
AsuNHI GCTAGC 2 cut(s) 169, 386
BbvCI CCTCAGC 1 cut(s) 275
BbvI GCAGC 5 cut(s) 157, 160, 258, 335, 402
BceAI ACGGC 1 cut(s) 73
BclI TGATCA 3 cut(s) 427, 442, 466
BcoDI GTCTC 1 cut(s) 380
BcuI ACTAGT 1 cut(s) 304
BfaI CTAG 3 cut(s) 170, 305, 387
BfmI CTRYAG 1 cut(s) 57
BisI GCNGC 5 cut(s) 146, 149, 272, 324, 391
BlsI GCNGC 5 cut(s) 147, 150, 273, 325, 392
BmtI GCTAGC 2 cut(s) 173, 390
Bpu10I CCTNAGC 1 cut(s) 275
Bse1I ACTGG 1 cut(s) 39
Bse3DI GCAATG 1 cut(s) 381
BseGI GGATG 2 cut(s) 52, 204
BseMI GCAATG 1 cut(s) 381
BseMII CTCAG 2 cut(s) 266, 318
BseNI ACTGG 1 cut(s) 39
BsePI GCGCGC 1 cut(s) 141
BseXI GCAGC 5 cut(s) 157, 160, 258, 335, 402
BseYI CCCAGC 1 cut(s) 313
Bsh1236I CGCG 2 cut(s) 141, 143
BslFI GGGAC 2 cut(s) 349, 450
BsmAI GTCTC 1 cut(s) 380
BsmFI GGGAC 2 cut(s) 349, 450
BsmI GAATGC 1 cut(s) 182
Bsp1407I TGTACA 1 cut(s) 228
Bsp143I GATC 3 cut(s) 427, 442, 466
BspACI CCGC 1 cut(s) 139
BspCNI CTCAG 2 cut(s) 267, 319
BspFNI CGCG 2 cut(s) 141, 143
BspHI TCATGA 1 cut(s) 342
BspOI GCTAGC 2 cut(s) 173, 390
BsrDI GCAATG 1 cut(s) 381
BsrGI TGTACA 1 cut(s) 228
BsrI ACTGG 1 cut(s) 39
BssHII GCGCGC 1 cut(s) 141
BssMI GATC 3 cut(s) 427, 442, 466
Bst4CI ACNGT 4 cut(s) 218, 233, 355, 374
BstAUI TGTACA 1 cut(s) 228
BstC8I GCNNGC 4 cut(s) 86, 143, 171, 388
BstDEI CTNAG 2 cut(s) 275, 327
BstF5I GGATG 2 cut(s) 52, 204
BstFNI CGCG 2 cut(s) 141, 143
BstHHI GCGC 2 cut(s) 143, 145
BstKTI GATC 3 cut(s) 430, 445, 469
BstMAI GTCTC 1 cut(s) 380
BstMBI GATC 3 cut(s) 427, 442, 466
BstMWI GCNNNNNNNGC 1 cut(s) 179
BstSFI CTRYAG 1 cut(s) 57
BstUI CGCG 2 cut(s) 141, 143
BstV1I GCAGC 5 cut(s) 157, 160, 258, 335, 402
BtsCI GGATG 2 cut(s) 52, 204
Cac8I GCNNGC 4 cut(s) 86, 143, 171, 388
CciI TCATGA 1 cut(s) 342
CfoI GCGC 2 cut(s) 143, 145
CseI GACGC 1 cut(s) 406
Csp6I GTAC 4 cut(s) 136, 229, 370, 473
CviAII CATG 5 cut(s) 133, 343, 440, 464, 470
CviJI RGCY 6 cut(s) 20, 84, 173, 271, 326, 393
CviKI_1 RGCY 6 cut(s) 20, 84, 173, 271, 326, 393
CviQI GTAC 4 cut(s) 136, 229, 370, 473
DdeI CTNAG 2 cut(s) 275, 327
DpnI GATC 3 cut(s) 429, 444, 468
DpnII GATC 3 cut(s) 427, 442, 466
DraIII CACNNNGTG 1 cut(s) 288
FaeI CATG 5 cut(s) 136, 346, 443, 467, 473
FaiI YATR 9 cut(s) 134, 213, 344, 368, 408, 423, 441, 465, 471
FaqI GGGAC 2 cut(s) 349, 450
FatI CATG 5 cut(s) 132, 342, 439, 463, 469
FbaI TGATCA 3 cut(s) 427, 442, 466
FblI GTMKAC 2 cut(s) 189, 235
Fnu4HI GCNGC 5 cut(s) 146, 149, 272, 324, 391
FokI GGATG 2 cut(s) 39, 191
Fsp4HI GCNGC 5 cut(s) 146, 149, 272, 324, 391
FspBI CTAG 3 cut(s) 170, 305, 387
GlaI GCGC 2 cut(s) 142, 144
GluI GCNGC 5 cut(s) 146, 149, 272, 324, 391
GsaI CCCAGC 1 cut(s) 317
HgaI GACGC 1 cut(s) 406
HhaI GCGC 2 cut(s) 143, 145
Hin1II CATG 5 cut(s) 136, 346, 443, 467, 473
Hin6I GCGC 2 cut(s) 141, 143
HinP1I GCGC 2 cut(s) 141, 143
HincII GTYRAC 1 cut(s) 236
HindII GTYRAC 1 cut(s) 236
HphI GGTGA 1 cut(s) 274
Hpy166II GTNNAC 5 cut(s) 190, 229, 236, 282, 412
Hpy188I TCNGA 1 cut(s) 447
Hpy188III TCNNGA 1 cut(s) 343
Hpy8I GTNNAC 5 cut(s) 190, 229, 236, 282, 412
HpyAV CCTTC 1 cut(s) 133
HpyCH4III ACNGT 4 cut(s) 218, 233, 355, 374
HpyCH4IV ACGT 1 cut(s) 348
HpyCH4V TGCA 2 cut(s) 223, 323
HpyF10VI GCNNNNNNNGC 1 cut(s) 179
HpyF3I CTNAG 2 cut(s) 275, 327
HpySE526I ACGT 1 cut(s) 348
Hsp92II CATG 5 cut(s) 136, 346, 443, 467, 473
HspAI GCGC 2 cut(s) 141, 143
Ksp22I TGATCA 3 cut(s) 427, 442, 466
Kzo9I GATC 3 cut(s) 427, 442, 466
LpnPI CCDG 5 cut(s) 43, 52, 299, 299, 323
Lsp1109I GCAGC 5 cut(s) 157, 160, 258, 335, 402
MaeI CTAG 3 cut(s) 170, 305, 387
MaeII ACGT 1 cut(s) 348
MaeIII GTNAC 2 cut(s) 349, 452
MalI GATC 3 cut(s) 429, 444, 468
MboI GATC 3 cut(s) 427, 442, 466
MboII GAAGA 1 cut(s) 274
MfeI CAATTG 1 cut(s) 477
MluCI AATT 2 cut(s) 71, 477
MnlI CCTC 7 cut(s) 32, 60, 119, 188, 205, 218, 270
MseI TTAA 1 cut(s) 105
MslI CAYNNNNRTG 4 cut(s) 131, 216, 347, 468
MspA1I CMGCKG 1 cut(s) 326
MunI CAATTG 1 cut(s) 477
Mva1269I GAATGC 1 cut(s) 182
MvnI CGCG 2 cut(s) 141, 143
MwoI GCNNNNNNNGC 1 cut(s) 179
NdeII GATC 3 cut(s) 427, 442, 466
NheI GCTAGC 2 cut(s) 169, 386
NlaIII CATG 5 cut(s) 136, 346, 443, 467, 473
NmuCI GTSAC 1 cut(s) 349
PagI TCATGA 1 cut(s) 342
PauI GCGCGC 1 cut(s) 141
PctI GAATGC 1 cut(s) 182
PkrI GCNGC 5 cut(s) 147, 150, 273, 325, 392
PspFI CCCAGC 1 cut(s) 313
PteI GCGCGC 1 cut(s) 141
PvuII CAGCTG 1 cut(s) 326
RsaI GTAC 4 cut(s) 137, 230, 371, 474
RsaNI GTAC 4 cut(s) 136, 229, 370, 473
RseI CAYNNNNRTG 4 cut(s) 131, 216, 347, 468
SalI GTCGAC 1 cut(s) 234
SaqAI TTAA 1 cut(s) 105
SatI GCNGC 5 cut(s) 146, 149, 272, 324, 391
Sau3AI GATC 3 cut(s) 427, 442, 466
SfcI CTRYAG 1 cut(s) 57
SmiMI CAYNNNNRTG 4 cut(s) 131, 216, 347, 468
SpeI ACTAGT 1 cut(s) 304
Sse9I AATT 2 cut(s) 71, 477
SsiI CCGC 1 cut(s) 139
SspMI CTAG 3 cut(s) 170, 305, 387
TaaI ACNGT 4 cut(s) 218, 233, 355, 374
TaiI ACGT 1 cut(s) 351
TaqI TCGA 1 cut(s) 235
TasI AATT 2 cut(s) 71, 477
TatI WGTACW 2 cut(s) 228, 369
Tru1I TTAA 1 cut(s) 105
Tru9I TTAA 1 cut(s) 105
TseFI GTSAC 1 cut(s) 349
TseI GCWGC 5 cut(s) 145, 148, 271, 323, 390
Tsp45I GTSAC 1 cut(s) 349
TspDTI ATGAA 4 cut(s) 91, 193, 331, 359
XmiI GTMKAC 2 cut(s) 189, 235
XspI CTAG 3 cut(s) 170, 305, 387
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.