Rh2BG622500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0014779
Physical Location & Seq
Forward (+)
84486502 .. 84486990
489 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG622500.1

Sequence Viewer

Length: 489 bp
ATGCCAAACACAAAACCAGCTCTCTCTACTAGCAACACCAATCGCCACGCTCAATTTACTATTAGCTTGCCATCCGTTGTGTTCATCAACCTCACAGTAACAACCTTCACAACCATGTACCGCGCGCAGCAGCACCACGACACCACCTTGCTCGCTTTCCTTGCATTCGTCTACGCGTCCTATTTCATCCTCCATAACTGTGTAGAAGTTTACAGTCGACTGCCCAAGGGCGAAAAGTCGACGAAGAAGGAGCTGCTGAGGTTCACTCTGTGGTTTCTGCCAACTGCAGTGCTGTTGGGATTTGCAGCTGAGTTTGGGACTTTCATGAACGTGACTGTGGTTGTGCTCATGTACTGTCTCGCCATTGCTAGCAGCTTGTTGCTGTTCTATGTCCACTACATTCATGACGATCAAAAGGAACATGCTTGTCCTATTCAGAAGAGTAACAACAAGCATGATCATGTACCAATTGCCCACAACATTGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

162

Amino Acids

18.45

Weight (kDa)

8.79

Isoelectric Point (pI)

41.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6490 PF20100 29 - 133 8.6e-07 Family of unknown function (DUF6490)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 171, 217, 239
AccII CGCG 3 cut(s) 123, 125, 176
AciI CCGC 1 cut(s) 121
AdeI CACNNNGTG 1 cut(s) 270
AfaI GTAC 3 cut(s) 119, 353, 465
AflIII ACRYGT 1 cut(s) 174
AluBI AGCT 5 cut(s) 20, 66, 253, 308, 375
AluI AGCT 5 cut(s) 20, 66, 253, 308, 375
Alw21I GWGCWC 1 cut(s) 348
Alw26I GTCTC 1 cut(s) 362
ApeKI GCWGC 5 cut(s) 127, 130, 253, 305, 372
AspLEI GCGC 2 cut(s) 125, 127
AsuNHI GCTAGC 1 cut(s) 368
Bbv12I GWGCWC 1 cut(s) 348
BbvCI CCTCAGC 1 cut(s) 257
BbvI GCAGC 5 cut(s) 139, 142, 240, 317, 384
BccI CCATC 1 cut(s) 79
BclI TGATCA 1 cut(s) 457
BcoDI GTCTC 1 cut(s) 362
BfaI CTAG 2 cut(s) 30, 369
BfmI CTRYAG 1 cut(s) 285
BisI GCNGC 5 cut(s) 128, 131, 254, 306, 373
BlsI GCNGC 5 cut(s) 129, 132, 255, 307, 374
BmtI GCTAGC 1 cut(s) 372
BplI GAGNNNNNCTC 2 cut(s) 250, 282
Bpu10I CCTNAGC 1 cut(s) 257
BsaJI CCNNGG 1 cut(s) 225
Bse3DI GCAATG 1 cut(s) 363
BseDI CCNNGG 1 cut(s) 225
BseGI GGATG 2 cut(s) 71, 186
BseMI GCAATG 1 cut(s) 363
BseMII CTCAG 2 cut(s) 248, 300
BsePI GCGCGC 1 cut(s) 123
BseXI GCAGC 5 cut(s) 139, 142, 240, 317, 384
Bsh1236I CGCG 3 cut(s) 123, 125, 176
BsiHKAI GWGCWC 1 cut(s) 348
BslFI GGGAC 1 cut(s) 331
BsmAI GTCTC 1 cut(s) 362
BsmFI GGGAC 1 cut(s) 331
BsmI GAATGC 1 cut(s) 164
Bsp1286I GDGCHC 1 cut(s) 348
Bsp143I GATC 2 cut(s) 409, 457
BspACI CCGC 1 cut(s) 121
BspCNI CTCAG 2 cut(s) 249, 301
BspFNI CGCG 3 cut(s) 123, 125, 176
BspHI TCATGA 2 cut(s) 324, 403
BspMAI CTGCAG 1 cut(s) 289
BspOI GCTAGC 1 cut(s) 372
BsrDI GCAATG 1 cut(s) 363
BssECI CCNNGG 1 cut(s) 225
BssHII GCGCGC 1 cut(s) 123
BssMI GATC 2 cut(s) 409, 457
BssT1I CCWWGG 1 cut(s) 225
Bst4CI ACNGT 5 cut(s) 97, 200, 215, 337, 356
Bst6I CTCTTC 1 cut(s) 434
BstC8I GCNNGC 4 cut(s) 68, 125, 153, 370
BstDEI CTNAG 2 cut(s) 257, 309
BstF5I GGATG 2 cut(s) 71, 186
BstFNI CGCG 3 cut(s) 123, 125, 176
BstHHI GCGC 2 cut(s) 125, 127
BstKTI GATC 2 cut(s) 412, 460
BstMAI GTCTC 1 cut(s) 362
BstMBI GATC 2 cut(s) 409, 457
BstMWI GCNNNNNNNGC 1 cut(s) 161
BstNSI RCATGY 1 cut(s) 425
BstSFI CTRYAG 1 cut(s) 285
BstUI CGCG 3 cut(s) 123, 125, 176
BstV1I GCAGC 5 cut(s) 139, 142, 240, 317, 384
BtsCI GGATG 2 cut(s) 71, 186
BtsI GCAGTG 1 cut(s) 294
BtsIMutI CAGTG 1 cut(s) 294
Cac8I GCNNGC 4 cut(s) 68, 125, 153, 370
CciI TCATGA 2 cut(s) 324, 403
CfoI GCGC 2 cut(s) 125, 127
CseI GACGC 1 cut(s) 165
Csp6I GTAC 3 cut(s) 118, 352, 464
CviAII CATG 7 cut(s) 115, 325, 349, 404, 422, 455, 461
CviJI RGCY 5 cut(s) 20, 66, 253, 308, 375
CviKI_1 RGCY 5 cut(s) 20, 66, 253, 308, 375
CviQI GTAC 3 cut(s) 118, 352, 464
DdeI CTNAG 2 cut(s) 257, 309
DpnI GATC 2 cut(s) 411, 459
DpnII GATC 2 cut(s) 409, 457
DraIII CACNNNGTG 1 cut(s) 270
Eam1104I CTCTTC 1 cut(s) 434
EarI CTCTTC 1 cut(s) 434
Eco130I CCWWGG 1 cut(s) 225
EcoT14I CCWWGG 1 cut(s) 225
ErhI CCWWGG 1 cut(s) 225
FaeI CATG 7 cut(s) 118, 328, 352, 407, 425, 458, 464
FaiI YATR 9 cut(s) 116, 195, 326, 350, 390, 405, 423, 456, 462
FaqI GGGAC 1 cut(s) 331
FatI CATG 7 cut(s) 114, 324, 348, 403, 421, 454, 460
FbaI TGATCA 1 cut(s) 457
FblI GTMKAC 3 cut(s) 171, 217, 239
Fnu4HI GCNGC 5 cut(s) 128, 131, 254, 306, 373
FokI GGATG 2 cut(s) 58, 173
Fsp4HI GCNGC 5 cut(s) 128, 131, 254, 306, 373
FspBI CTAG 2 cut(s) 30, 369
GlaI GCGC 2 cut(s) 124, 126
GluI GCNGC 5 cut(s) 128, 131, 254, 306, 373
HgaI GACGC 1 cut(s) 165
HhaI GCGC 2 cut(s) 125, 127
Hin1II CATG 7 cut(s) 118, 328, 352, 407, 425, 458, 464
Hin6I GCGC 2 cut(s) 123, 125
HinP1I GCGC 2 cut(s) 123, 125
HincII GTYRAC 2 cut(s) 218, 240
HindII GTYRAC 2 cut(s) 218, 240
Hpy166II GTNNAC 6 cut(s) 172, 211, 218, 240, 264, 394
Hpy188I TCNGA 1 cut(s) 438
Hpy188III TCNNGA 2 cut(s) 325, 404
Hpy8I GTNNAC 6 cut(s) 172, 211, 218, 240, 264, 394
Hpy99I CGWCG 1 cut(s) 244
HpyAV CCTTC 2 cut(s) 115, 241
HpyCH4III ACNGT 5 cut(s) 97, 200, 215, 337, 356
HpyCH4IV ACGT 1 cut(s) 330
HpyCH4V TGCA 3 cut(s) 164, 287, 305
HpyF10VI GCNNNNNNNGC 1 cut(s) 161
HpyF3I CTNAG 2 cut(s) 257, 309
HpySE526I ACGT 1 cut(s) 330
Hsp92II CATG 7 cut(s) 118, 328, 352, 407, 425, 458, 464
HspAI GCGC 2 cut(s) 123, 125
Ksp22I TGATCA 1 cut(s) 457
Kzo9I GATC 2 cut(s) 409, 457
LmnI GCTCC 1 cut(s) 250
LpnPI CCDG 1 cut(s) 30
Lsp1109I GCAGC 5 cut(s) 139, 142, 240, 317, 384
MaeI CTAG 2 cut(s) 30, 369
MaeII ACGT 1 cut(s) 330
MaeIII GTNAC 3 cut(s) 97, 331, 443
MalI GATC 2 cut(s) 411, 459
MboI GATC 2 cut(s) 409, 457
MboII GAAGA 2 cut(s) 256, 451
MfeI CAATTG 1 cut(s) 468
MhlI GDGCHC 1 cut(s) 348
MluCI AATT 2 cut(s) 53, 468
MluI ACGCGT 1 cut(s) 174
MnlI CCTC 3 cut(s) 101, 200, 252
MslI CAYNNNNRTG 4 cut(s) 113, 198, 329, 459
MspA1I CMGCKG 1 cut(s) 308
MunI CAATTG 1 cut(s) 468
Mva1269I GAATGC 1 cut(s) 164
MvnI CGCG 3 cut(s) 123, 125, 176
MwoI GCNNNNNNNGC 1 cut(s) 161
NdeII GATC 2 cut(s) 409, 457
NheI GCTAGC 1 cut(s) 368
NlaIII CATG 7 cut(s) 118, 328, 352, 407, 425, 458, 464
NmuCI GTSAC 1 cut(s) 331
NspI RCATGY 1 cut(s) 425
PagI TCATGA 2 cut(s) 324, 403
PauI GCGCGC 1 cut(s) 123
PctI GAATGC 1 cut(s) 164
PkrI GCNGC 5 cut(s) 129, 132, 255, 307, 374
PstI CTGCAG 1 cut(s) 289
PteI GCGCGC 1 cut(s) 123
PvuII CAGCTG 1 cut(s) 308
RsaI GTAC 3 cut(s) 119, 353, 465
RsaNI GTAC 3 cut(s) 118, 352, 464
RseI CAYNNNNRTG 4 cut(s) 113, 198, 329, 459
SalI GTCGAC 2 cut(s) 216, 238
SatI GCNGC 5 cut(s) 128, 131, 254, 306, 373
Sau3AI GATC 2 cut(s) 409, 457
SduI GDGCHC 1 cut(s) 348
SfcI CTRYAG 1 cut(s) 285
SmiMI CAYNNNNRTG 4 cut(s) 113, 198, 329, 459
Sse9I AATT 2 cut(s) 53, 468
SsiI CCGC 1 cut(s) 121
SspMI CTAG 2 cut(s) 30, 369
StyI CCWWGG 1 cut(s) 225
TaaI ACNGT 5 cut(s) 97, 200, 215, 337, 356
TaiI ACGT 1 cut(s) 333
TaqI TCGA 2 cut(s) 217, 239
TasI AATT 2 cut(s) 53, 468
TatI WGTACW 1 cut(s) 351
TscAI CASTG 1 cut(s) 294
TseFI GTSAC 1 cut(s) 331
TseI GCWGC 5 cut(s) 127, 130, 253, 305, 372
Tsp45I GTSAC 1 cut(s) 331
TspDTI ATGAA 5 cut(s) 73, 175, 313, 341, 392
TspGWI ACGGA 1 cut(s) 64
TspRI CASTG 1 cut(s) 294
XceI RCATGY 1 cut(s) 425
XmiI GTMKAC 3 cut(s) 171, 217, 239
XspI CTAG 2 cut(s) 30, 369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.