Prupe.3G272200_v2.0.a1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0014779
Physical Location & Seq
Forward (+)
25256193 .. 25256977
785 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G272200.1

Sequence Viewer

Length: 531 bp
ATGTTGCCAACTTATGATCAGAAGAAACCTACAAAAACAACTCTTCCTACTTTCAACACTCGCAACCCAGATGCTAGCCAAACAATAAACTTGACCCCATGCTTTGTTTACTTCAACCTCACAATTTGTTCCCTTACAACGCTCTACCGGGCGTACGAGCACAGCGACTATCCAGTGGTGGCTTTCGTCGTGTTCGTCTACGTGTCGTATTTCGTGTTGGACAAGTGTCTAGCAGTGTACACTCAGTTGCCAAGAGGCGAGCAATCCGTGAAGAAAGAGTTGTTGAGGTTCACCCTTTGGGGGTTGTCAAGCGCCATCCTGTTCGGGTTTGCATATCAGTTTGGGACGTTTATGCACTTGTCTGCGGTTGTGCTCATGTATTGTATTGCCGTTTCCAGCAGTTTGCTGCTCTTCTATGCCTACTACATCGTTGATGACCAAAATCACAACGGTTGCAGTTTTTGCGCCACCATACCTCCTCAGTCTTCTTGTCCTAACCAGGAAAAGCGTAACAATTCGGACAATGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

177

Amino Acids

20.04

Weight (kDa)

7.54

Isoelectric Point (pI)

41.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 198
AciI CCGC 1 cut(s) 365
AfaI GTAC 2 cut(s) 155, 239
AfiI CCNNNNNNNGG 1 cut(s) 300
AflIII ACRYGT 1 cut(s) 201
AgsI TTSAA 2 cut(s) 55, 115
AjnI CCWGG 1 cut(s) 498
Alw21I GWGCWC 2 cut(s) 162, 375
ApeKI GCWGC 1 cut(s) 406
AspLEI GCGC 2 cut(s) 314, 467
AsuC2I CCSGG 1 cut(s) 149
AsuHPI GGTGA 1 cut(s) 283
AsuNHI GCTAGC 1 cut(s) 74
BbsI GAAGAC 1 cut(s) 477
Bbv12I GWGCWC 2 cut(s) 162, 375
BbvI GCAGC 1 cut(s) 393
BccI CCATC 1 cut(s) 323
BceAI ACGGC 1 cut(s) 374
BciT130I CCWGG 1 cut(s) 500
BclI TGATCA 1 cut(s) 16
BcnI CCSGG 1 cut(s) 149
BfaI CTAG 3 cut(s) 75, 230, 529
BfoI RGCGCY 1 cut(s) 315
BisI GCNGC 1 cut(s) 407
BlsI GCNGC 1 cut(s) 408
Bme1390I CCNGG 2 cut(s) 149, 500
BmrFI CCNGG 2 cut(s) 149, 500
BmsI GCATC 1 cut(s) 61
BmtI GCTAGC 1 cut(s) 78
BoxI GACNNNNGTC 1 cut(s) 225
BpiI GAAGAC 1 cut(s) 477
BpuMI CCSGG 1 cut(s) 149
BsaAI YACGTR 1 cut(s) 202
BsaXI ACNNNNNCTCC 2 cut(s) 460, 490
Bsc4I CCNNNNNNNGG 1 cut(s) 300
Bse1I ACTGG 1 cut(s) 173
BseBI CCWGG 1 cut(s) 500
BseGI GGATG 1 cut(s) 315
BseLI CCNNNNNNNGG 1 cut(s) 300
BseMII CTCAG 2 cut(s) 257, 494
BseNI ACTGG 1 cut(s) 173
BseRI GAGGAG 1 cut(s) 468
BseXI GCAGC 1 cut(s) 393
BsiHKAI GWGCWC 2 cut(s) 162, 375
BsiSI CCGG 1 cut(s) 148
BsiWI CGTACG 1 cut(s) 153
BslFI GGGAC 1 cut(s) 358
BslI CCNNNNNNNGG 1 cut(s) 300
BsmFI GGGAC 1 cut(s) 358
Bsp1286I GDGCHC 2 cut(s) 162, 375
Bsp1407I TGTACA 1 cut(s) 237
Bsp143I GATC 1 cut(s) 16
BspACI CCGC 1 cut(s) 365
BspCNI CTCAG 2 cut(s) 256, 493
BspOI GCTAGC 1 cut(s) 78
BspQI GCTCTTC 1 cut(s) 416
BsrGI TGTACA 1 cut(s) 237
BsrI ACTGG 1 cut(s) 173
BssMI GATC 1 cut(s) 16
Bst2UI CCWGG 1 cut(s) 500
Bst4CI ACNGT 1 cut(s) 452
Bst6I CTCTTC 2 cut(s) 48, 416
BstAPI GCANNNNNTGC 1 cut(s) 462
BstAUI TGTACA 1 cut(s) 237
BstBAI YACGTR 1 cut(s) 202
BstC8I GCNNGC 2 cut(s) 76, 260
BstDEI CTNAG 2 cut(s) 243, 480
BstF5I GGATG 1 cut(s) 315
BstH2I RGCGCY 1 cut(s) 315
BstHHI GCGC 2 cut(s) 314, 467
BstKTI GATC 1 cut(s) 19
BstMBI GATC 1 cut(s) 16
BstMWI GCNNNNNNNGC 1 cut(s) 462
BstNI CCWGG 1 cut(s) 500
BstPAI GACNNNNGTC 1 cut(s) 225
BstSCI CCNGG 2 cut(s) 147, 498
BstV1I GCAGC 1 cut(s) 393
BstV2I GAAGAC 1 cut(s) 477
BtsCI GGATG 1 cut(s) 315
BtsI GCAGTG 1 cut(s) 240
BtsIMutI CAGTG 2 cut(s) 180, 240
Cac8I GCNNGC 2 cut(s) 76, 260
CfoI GCGC 2 cut(s) 314, 467
Csp6I GTAC 2 cut(s) 154, 238
CviAII CATG 2 cut(s) 99, 376
CviJI RGCY 2 cut(s) 78, 182
CviKI_1 RGCY 2 cut(s) 78, 182
CviQI GTAC 2 cut(s) 154, 238
DdeI CTNAG 2 cut(s) 243, 480
DpnI GATC 1 cut(s) 18
DpnII GATC 1 cut(s) 16
Eam1104I CTCTTC 2 cut(s) 48, 416
EarI CTCTTC 2 cut(s) 48, 416
EcoRII CCWGG 1 cut(s) 498
FaeI CATG 2 cut(s) 102, 379
FaiI YATR 7 cut(s) 15, 100, 334, 353, 377, 417, 473
FaqI GGGAC 1 cut(s) 358
FatI CATG 2 cut(s) 98, 375
FbaI TGATCA 1 cut(s) 16
FblI GTMKAC 1 cut(s) 198
Fnu4HI GCNGC 1 cut(s) 407
FokI GGATG 1 cut(s) 302
Fsp4HI GCNGC 1 cut(s) 407
FspBI CTAG 3 cut(s) 75, 230, 529
GlaI GCGC 2 cut(s) 313, 466
GluI GCNGC 1 cut(s) 407
HaeII RGCGCY 1 cut(s) 315
HapII CCGG 1 cut(s) 148
HhaI GCGC 2 cut(s) 314, 467
Hin1II CATG 2 cut(s) 102, 379
Hin6I GCGC 2 cut(s) 312, 465
HinP1I GCGC 2 cut(s) 312, 465
HpaII CCGG 1 cut(s) 148
HphI GGTGA 1 cut(s) 283
Hpy166II GTNNAC 5 cut(s) 109, 199, 238, 240, 291
Hpy188I TCNGA 2 cut(s) 21, 520
Hpy8I GTNNAC 5 cut(s) 109, 199, 238, 240, 291
Hpy99I CGWCG 1 cut(s) 191
HpyCH4III ACNGT 1 cut(s) 452
HpyCH4IV ACGT 2 cut(s) 201, 347
HpyCH4V TGCA 3 cut(s) 332, 355, 456
HpyF10VI GCNNNNNNNGC 1 cut(s) 462
HpyF3I CTNAG 2 cut(s) 243, 480
HpySE526I ACGT 2 cut(s) 201, 347
Hsp92II CATG 2 cut(s) 102, 379
HspAI GCGC 2 cut(s) 312, 465
Ksp22I TGATCA 1 cut(s) 16
Kzo9I GATC 1 cut(s) 16
LguI GCTCTTC 1 cut(s) 416
LpnPI CCDG 7 cut(s) 81, 161, 186, 332, 409, 485, 512
Lsp1109I GCAGC 1 cut(s) 393
LweI GCATC 1 cut(s) 61
MaeI CTAG 3 cut(s) 75, 230, 529
MaeII ACGT 2 cut(s) 201, 347
MaeIII GTNAC 1 cut(s) 509
MalI GATC 1 cut(s) 18
MboI GATC 1 cut(s) 16
MboII GAAGA 5 cut(s) 34, 35, 283, 403, 477
MhlI GDGCHC 2 cut(s) 162, 375
MluCI AATT 2 cut(s) 123, 514
MmeI TCCRAC 1 cut(s) 198
MnlI CCTC 5 cut(s) 128, 248, 279, 486, 489
MspI CCGG 1 cut(s) 148
MspR9I CCNGG 2 cut(s) 149, 500
MvaI CCWGG 1 cut(s) 500
MwoI GCNNNNNNNGC 1 cut(s) 462
NciI CCSGG 1 cut(s) 149
NdeII GATC 1 cut(s) 16
NheI GCTAGC 1 cut(s) 74
NlaIII CATG 2 cut(s) 102, 379
PciSI GCTCTTC 1 cut(s) 416
PcsI WCGNNNNNNNCGW 2 cut(s) 162, 192
Pfl23II CGTACG 1 cut(s) 153
PflFI GACNNNGTC 1 cut(s) 524
PkrI GCNGC 1 cut(s) 408
Ppu21I YACGTR 1 cut(s) 202
PshAI GACNNNNGTC 1 cut(s) 225
Psp6I CCWGG 1 cut(s) 498
PspGI CCWGG 1 cut(s) 498
PspLI CGTACG 1 cut(s) 153
PsyI GACNNNGTC 1 cut(s) 524
RsaI GTAC 2 cut(s) 155, 239
RsaNI GTAC 2 cut(s) 154, 238
SapI GCTCTTC 1 cut(s) 416
SatI GCNGC 1 cut(s) 407
Sau3AI GATC 1 cut(s) 16
ScrFI CCNGG 2 cut(s) 149, 500
SduI GDGCHC 2 cut(s) 162, 375
SetI ASST 6 cut(s) 31, 120, 204, 290, 350, 478
SfaNI GCATC 1 cut(s) 61
Sse9I AATT 2 cut(s) 123, 514
SsiI CCGC 1 cut(s) 365
SspMI CTAG 3 cut(s) 75, 230, 529
StyD4I CCNGG 2 cut(s) 147, 498
TaaI ACNGT 1 cut(s) 452
TaiI ACGT 2 cut(s) 204, 350
TasI AATT 2 cut(s) 123, 514
TatI WGTACW 1 cut(s) 237
TscAI CASTG 2 cut(s) 180, 240
TseI GCWGC 1 cut(s) 406
TspGWI ACGGA 1 cut(s) 256
TspRI CASTG 2 cut(s) 180, 240
Tth111I GACNNNGTC 1 cut(s) 524
XmiI GTMKAC 1 cut(s) 198
XspI CTAG 3 cut(s) 75, 230, 529
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.