RchiOBHm_Chr2g0169181

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0014779
Physical Location & Seq
Forward (+)
83429828 .. 83430400
573 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ53681

Sequence Viewer

Length: 504 bp
ATGCCAAACACAAAACCAGCTCTCCCTACCAGCAACACCAATCGCCACCCTCCCACAGATACCGCTCAATTTACCATTAGCTTGCCGTCCGTTGTGTTCATTAACCTCACAGTAACAACCTTCACAACCATGTACCGCGCGCAGCAGCACCACGACACCACCTTGCTCGCTTTCCTTGCATTCGTCTACGCGTCCTATTTCATCCTCCATAACTGTGTAGAAGTTTACAGTCGACTGCCCAAGGGCGAAAAGTCGACGAAGAAAGAGCTGCTGAGGTTCACTCTGTGGTTTCTGCCAACTGCAGTGCTGTTGGGATTTGCAGCTGAGTTTGGGACTTTCATGAACGTGACTGTGGTTGTGCTCATGTACTGTCTCGCCATTGCTAGCAGCTTGTTGCTGTTCTATGTCCACTACATTCATGACGATCAAAAGGGACATGCTTGTCCAACTCAGAAGAGTAACAACAAGCATGATCATGTACCAATTGCCCAAAACATTGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

167

Amino Acids

18.88

Weight (kDa)

8.79

Isoelectric Point (pI)

40.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6490 PF20100 35 - 138 8.3e-07 Family of unknown function (DUF6490)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 441
AccBSI CCGCTC 1 cut(s) 65
AccI GTMKAC 3 cut(s) 186, 232, 254
AccII CGCG 3 cut(s) 138, 140, 191
AciI CCGC 2 cut(s) 63, 136
AdeI CACNNNGTG 1 cut(s) 285
AfaI GTAC 3 cut(s) 134, 368, 480
AflIII ACRYGT 1 cut(s) 189
AluBI AGCT 5 cut(s) 20, 81, 268, 323, 390
AluI AGCT 5 cut(s) 20, 81, 268, 323, 390
Alw21I GWGCWC 1 cut(s) 363
Alw26I GTCTC 1 cut(s) 377
ApeKI GCWGC 5 cut(s) 142, 145, 268, 320, 387
AspLEI GCGC 2 cut(s) 140, 142
AsuNHI GCTAGC 1 cut(s) 383
Bbv12I GWGCWC 1 cut(s) 363
BbvCI CCTCAGC 1 cut(s) 272
BbvI GCAGC 5 cut(s) 154, 157, 255, 332, 399
BceAI ACGGC 1 cut(s) 70
BclI TGATCA 1 cut(s) 472
BcoDI GTCTC 1 cut(s) 377
BfaI CTAG 1 cut(s) 384
BfmI CTRYAG 1 cut(s) 300
BisI GCNGC 5 cut(s) 143, 146, 269, 321, 388
BlsI GCNGC 5 cut(s) 144, 147, 270, 322, 389
BmtI GCTAGC 1 cut(s) 387
BplI GAGNNNNNCTC 2 cut(s) 265, 297
Bpu10I CCTNAGC 1 cut(s) 272
BsaJI CCNNGG 1 cut(s) 240
BsaXI ACNNNNNCTCC 2 cut(s) 6, 36
Bse3DI GCAATG 1 cut(s) 378
BseDI CCNNGG 1 cut(s) 240
BseGI GGATG 1 cut(s) 201
BseMI GCAATG 1 cut(s) 378
BseMII CTCAG 3 cut(s) 263, 315, 464
BsePI GCGCGC 1 cut(s) 138
BseXI GCAGC 5 cut(s) 154, 157, 255, 332, 399
Bsh1236I CGCG 3 cut(s) 138, 140, 191
BsiHKAI GWGCWC 1 cut(s) 363
BslFI GGGAC 2 cut(s) 346, 447
BsmAI GTCTC 1 cut(s) 377
BsmFI GGGAC 2 cut(s) 346, 447
BsmI GAATGC 1 cut(s) 179
Bsp1286I GDGCHC 1 cut(s) 363
Bsp143I GATC 2 cut(s) 424, 472
BspACI CCGC 2 cut(s) 63, 136
BspCNI CTCAG 3 cut(s) 264, 316, 463
BspFNI CGCG 3 cut(s) 138, 140, 191
BspHI TCATGA 2 cut(s) 339, 418
BspMAI CTGCAG 1 cut(s) 304
BspOI GCTAGC 1 cut(s) 387
BsrBI CCGCTC 1 cut(s) 65
BsrDI GCAATG 1 cut(s) 378
BssECI CCNNGG 1 cut(s) 240
BssHII GCGCGC 1 cut(s) 138
BssMI GATC 2 cut(s) 424, 472
BssT1I CCWWGG 1 cut(s) 240
Bst4CI ACNGT 5 cut(s) 112, 215, 230, 352, 371
Bst6I CTCTTC 1 cut(s) 449
BstC8I GCNNGC 4 cut(s) 83, 140, 168, 385
BstDEI CTNAG 3 cut(s) 272, 324, 450
BstF5I GGATG 1 cut(s) 201
BstFNI CGCG 3 cut(s) 138, 140, 191
BstHHI GCGC 2 cut(s) 140, 142
BstKTI GATC 2 cut(s) 427, 475
BstMAI GTCTC 1 cut(s) 377
BstMBI GATC 2 cut(s) 424, 472
BstMWI GCNNNNNNNGC 1 cut(s) 176
BstNSI RCATGY 1 cut(s) 440
BstSFI CTRYAG 1 cut(s) 300
BstUI CGCG 3 cut(s) 138, 140, 191
BstV1I GCAGC 5 cut(s) 154, 157, 255, 332, 399
BtsCI GGATG 1 cut(s) 201
BtsI GCAGTG 1 cut(s) 309
BtsIMutI CAGTG 1 cut(s) 309
Cac8I GCNNGC 4 cut(s) 83, 140, 168, 385
CciI TCATGA 2 cut(s) 339, 418
CfoI GCGC 2 cut(s) 140, 142
CseI GACGC 1 cut(s) 180
Csp6I GTAC 3 cut(s) 133, 367, 479
CviAII CATG 7 cut(s) 130, 340, 364, 419, 437, 470, 476
CviJI RGCY 5 cut(s) 20, 81, 268, 323, 390
CviKI_1 RGCY 5 cut(s) 20, 81, 268, 323, 390
CviQI GTAC 3 cut(s) 133, 367, 479
DdeI CTNAG 3 cut(s) 272, 324, 450
DpnI GATC 2 cut(s) 426, 474
DpnII GATC 2 cut(s) 424, 472
DraIII CACNNNGTG 1 cut(s) 285
DrdI GACNNNNNNGTC 1 cut(s) 441
DseDI GACNNNNNNGTC 1 cut(s) 441
Eam1104I CTCTTC 1 cut(s) 449
EarI CTCTTC 1 cut(s) 449
Eco130I CCWWGG 1 cut(s) 240
EcoT14I CCWWGG 1 cut(s) 240
ErhI CCWWGG 1 cut(s) 240
FaeI CATG 7 cut(s) 133, 343, 367, 422, 440, 473, 479
FaiI YATR 9 cut(s) 131, 210, 341, 365, 405, 420, 438, 471, 477
FaqI GGGAC 2 cut(s) 346, 447
FatI CATG 7 cut(s) 129, 339, 363, 418, 436, 469, 475
FbaI TGATCA 1 cut(s) 472
FblI GTMKAC 3 cut(s) 186, 232, 254
Fnu4HI GCNGC 5 cut(s) 143, 146, 269, 321, 388
FokI GGATG 1 cut(s) 188
Fsp4HI GCNGC 5 cut(s) 143, 146, 269, 321, 388
FspBI CTAG 1 cut(s) 384
GlaI GCGC 2 cut(s) 139, 141
GluI GCNGC 5 cut(s) 143, 146, 269, 321, 388
HgaI GACGC 1 cut(s) 180
HhaI GCGC 2 cut(s) 140, 142
Hin1II CATG 7 cut(s) 133, 343, 367, 422, 440, 473, 479
Hin6I GCGC 2 cut(s) 138, 140
HinP1I GCGC 2 cut(s) 138, 140
HincII GTYRAC 2 cut(s) 233, 255
HindII GTYRAC 2 cut(s) 233, 255
Hpy166II GTNNAC 6 cut(s) 187, 226, 233, 255, 279, 409
Hpy188I TCNGA 1 cut(s) 453
Hpy188III TCNNGA 2 cut(s) 340, 419
Hpy8I GTNNAC 6 cut(s) 187, 226, 233, 255, 279, 409
Hpy99I CGWCG 1 cut(s) 259
HpyAV CCTTC 1 cut(s) 130
HpyCH4III ACNGT 5 cut(s) 112, 215, 230, 352, 371
HpyCH4IV ACGT 1 cut(s) 345
HpyCH4V TGCA 3 cut(s) 179, 302, 320
HpyF10VI GCNNNNNNNGC 1 cut(s) 176
HpyF3I CTNAG 3 cut(s) 272, 324, 450
HpySE526I ACGT 1 cut(s) 345
Hsp92II CATG 7 cut(s) 133, 343, 367, 422, 440, 473, 479
HspAI GCGC 2 cut(s) 138, 140
Ksp22I TGATCA 1 cut(s) 472
Kzo9I GATC 2 cut(s) 424, 472
LpnPI CCDG 2 cut(s) 30, 43
Lsp1109I GCAGC 5 cut(s) 154, 157, 255, 332, 399
MaeI CTAG 1 cut(s) 384
MaeII ACGT 1 cut(s) 345
MaeIII GTNAC 3 cut(s) 112, 346, 458
MalI GATC 2 cut(s) 426, 474
MbiI CCGCTC 1 cut(s) 65
MboI GATC 2 cut(s) 424, 472
MboII GAAGA 2 cut(s) 271, 466
MfeI CAATTG 1 cut(s) 483
MhlI GDGCHC 1 cut(s) 363
MluCI AATT 2 cut(s) 68, 483
MluI ACGCGT 1 cut(s) 189
MmeI TCCRAC 1 cut(s) 470
MnlI CCTC 4 cut(s) 60, 116, 215, 267
MseI TTAA 1 cut(s) 102
MslI CAYNNNNRTG 4 cut(s) 128, 213, 344, 474
MspA1I CMGCKG 1 cut(s) 323
MunI CAATTG 1 cut(s) 483
Mva1269I GAATGC 1 cut(s) 179
MvnI CGCG 3 cut(s) 138, 140, 191
MwoI GCNNNNNNNGC 1 cut(s) 176
NdeII GATC 2 cut(s) 424, 472
NheI GCTAGC 1 cut(s) 383
NlaIII CATG 7 cut(s) 133, 343, 367, 422, 440, 473, 479
NmuCI GTSAC 1 cut(s) 346
NspI RCATGY 1 cut(s) 440
PagI TCATGA 2 cut(s) 339, 418
PauI GCGCGC 1 cut(s) 138
PctI GAATGC 1 cut(s) 179
PkrI GCNGC 5 cut(s) 144, 147, 270, 322, 389
PstI CTGCAG 1 cut(s) 304
PteI GCGCGC 1 cut(s) 138
PvuII CAGCTG 1 cut(s) 323
RsaI GTAC 3 cut(s) 134, 368, 480
RsaNI GTAC 3 cut(s) 133, 367, 479
RseI CAYNNNNRTG 4 cut(s) 128, 213, 344, 474
SalI GTCGAC 2 cut(s) 231, 253
SaqAI TTAA 1 cut(s) 102
SatI GCNGC 5 cut(s) 143, 146, 269, 321, 388
Sau3AI GATC 2 cut(s) 424, 472
SduI GDGCHC 1 cut(s) 363
SfcI CTRYAG 1 cut(s) 300
SmiMI CAYNNNNRTG 4 cut(s) 128, 213, 344, 474
Sse9I AATT 2 cut(s) 68, 483
SsiI CCGC 2 cut(s) 63, 136
SspMI CTAG 1 cut(s) 384
StyI CCWWGG 1 cut(s) 240
TaaI ACNGT 5 cut(s) 112, 215, 230, 352, 371
TaiI ACGT 1 cut(s) 348
TaqI TCGA 2 cut(s) 232, 254
TasI AATT 2 cut(s) 68, 483
TatI WGTACW 1 cut(s) 366
Tru1I TTAA 1 cut(s) 102
Tru9I TTAA 1 cut(s) 102
TscAI CASTG 1 cut(s) 309
TseFI GTSAC 1 cut(s) 346
TseI GCWGC 5 cut(s) 142, 145, 268, 320, 387
Tsp45I GTSAC 1 cut(s) 346
TspDTI ATGAA 5 cut(s) 88, 190, 328, 356, 407
TspGWI ACGGA 1 cut(s) 79
TspRI CASTG 1 cut(s) 309
XceI RCATGY 1 cut(s) 440
XmiI GTMKAC 3 cut(s) 186, 232, 254
XspI CTAG 1 cut(s) 384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.