FvH4_6g54030
NAC Family

inactive shikimate kinase like 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0013212
Physical Location & Seq
Forward (+)
39503183 .. 39505927
2745 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g54030.t1

Sequence Viewer

Length: 882 bp
ATGGTGGCCATGGAGATTACGAGGACTCTCCTCCCGTCCTCCTCCTTCTCTAACCTCCGCCGCGCTCCTCCGTTATTCCATTTTCACGTCTCGCCGTCGAGTTCGTTATGCCCAGATGTCCTTCACTCTCAGATCTCACTCCCCATTACCTCTCGCTCTCACTTCCGTAAACCTCTCGCCGTCTCCGATGGCAGCGCTTCCGCAACCAAGGTTGCTGTAGATGATCCCTCTCTTGAAGTGAAGAAGAAGGCAATGGACGTGTCACCTGAACTCAAAGGAACTACTATATTCCTTGTCGGAATGAAGAGCTCCATAAAGACCAGTTTGGGGAAATTCCTTGCCAATGTGTTACGATACTATTACTTCGACAGTGATAGCTTGGTTGAGGAAGCTGCTGGCACTCTATCTGCCGCCAAATCTTTACGGGAGGTTGACAAGAGCGGTTTCCAAGAGTCTGAGACTGAAGTGTTGAAGCAATTATCATCCATGGGTCGGTTAGTGGTCTGTGCTGGAGATGGTGCAACTCAGAGTTCAACAAATCTTGCTCTTTTAAGACATGGGATCACAATATGGATTGACGTGCCTTTAGACATTGTGGCCAGGGGTATGGTCGAAGATCAAACTCAGCTCTCTGCATACGATCTATCTACTTCGGTATCTTATCCAGAGGTTTTGACTCGCTTAAGTACTGCATATGAAGAGATGAGAGGTGGATATGCAATAGCTGATGCAACCGTATCTGTTCAGCAAGTAGCTGGTAAGTTAGGTTATGATGAAATTGATGATGTGACCACCGAAGACATGGTTCTGGAGGTTCTCAAGGAGGTAGAGAAACTGACTAGAGTGAAGAAGATGATGGAAGCAGCAGCAAGACCCTTTTAA

Protein Analysis

294

Amino Acids

31.89

Weight (kDa)

5.49

Isoelectric Point (pI)

40.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SKI PF01202 104 - 225 4.4e-23 Shikimate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013212)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26900 AT3G26900 AT3G26900
fragaria_vesca FvH4_6g54030 FvH4_6g54030
malus_domestica MD09G1006100.v1.1 MD17G1010100.v1.1
prunus_persica Prupe.3G310900_v2.0.a1
pyrus_communis pycom17g00460
rosa_chinensis RchiOBHm_Chr2g0176211
rosa_laevigata RLG00000022390
rosa_multiflora Rmu_co8135912.1_g000001
rosa_rugosa Rorug02G0591800
rosa_samantha Rh2AG670400 Rh2BG681900 Rh2CG644700 Rh2DG695500
rosa_wichuraiana Rw2G054990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 441
AccII CGCG 1 cut(s) 63
AciI CCGC 5 cut(s) 58, 61, 201, 411, 441
AclWI GGATC 2 cut(s) 218, 569
AcoI YGGCCR 2 cut(s) 6, 597
AcsI RAATTY 1 cut(s) 332
AcuI CTGAAG 1 cut(s) 483
AfaI GTAC 1 cut(s) 688
AfeI AGCGCT 1 cut(s) 196
AfiI CCNNNNNNNGG 2 cut(s) 327, 492
AflII CTTAAG 1 cut(s) 682
AflIII ACRYGT 1 cut(s) 258
AgsI TTSAA 3 cut(s) 236, 472, 534
AjiI CACGTC 3 cut(s) 88, 259, 580
AjnI CCWGG 1 cut(s) 599
AluBI AGCT 6 cut(s) 309, 378, 392, 628, 725, 755
AluI AGCT 6 cut(s) 309, 378, 392, 628, 725, 755
Alw21I GWGCWC 1 cut(s) 311
Alw26I GTCTC 3 cut(s) 94, 187, 452
AlwI GGATC 2 cut(s) 218, 569
Aor51HI AGCGCT 1 cut(s) 196
AoxI GGCC 2 cut(s) 6, 597
ApeKI GCWGC 4 cut(s) 192, 392, 863, 866
ApoI RAATTY 1 cut(s) 332
AspLEI GCGC 2 cut(s) 65, 197
AsuHPI GGTGA 1 cut(s) 255
BaeI ACNNNNGTAYC 2 cut(s) 639, 672
BalI TGGCCA 2 cut(s) 8, 599
BanII GRGCYC 1 cut(s) 311
BbsI GAAGAC 1 cut(s) 804
Bbv12I GWGCWC 1 cut(s) 311
BbvI GCAGC 4 cut(s) 204, 379, 875, 878
BccI CCATC 3 cut(s) 182, 509, 850
BceAI ACGGC 2 cut(s) 79, 164
BciT130I CCWGG 1 cut(s) 601
BcoDI GTCTC 3 cut(s) 94, 187, 452
BfaI CTAG 1 cut(s) 840
BfmI CTRYAG 1 cut(s) 216
BfoI RGCGCY 1 cut(s) 198
BfrI CTTAAG 1 cut(s) 682
BglII AGATCT 1 cut(s) 132
BisI GCNGC 6 cut(s) 61, 193, 393, 411, 864, 867
BlsI GCNGC 6 cut(s) 62, 194, 394, 412, 865, 868
BmcAI AGTACT 1 cut(s) 688
Bme1390I CCNGG 1 cut(s) 601
BmgBI CACGTC 3 cut(s) 88, 259, 580
BmrFI CCNGG 1 cut(s) 601
BmsI GCATC 1 cut(s) 718
BpiI GAAGAC 1 cut(s) 804
BpmI CTGGAG 2 cut(s) 531, 830
BpuEI CTTGAG 1 cut(s) 803
BsaJI CCNNGG 4 cut(s) 9, 207, 486, 600
Bsc4I CCNNNNNNNGG 2 cut(s) 327, 492
Bse1I ACTGG 1 cut(s) 321
Bse3DI GCAATG 1 cut(s) 258
BseBI CCWGG 1 cut(s) 601
BseDI CCNNGG 4 cut(s) 9, 207, 486, 600
BseGI GGATG 1 cut(s) 482
BseLI CCNNNNNNNGG 2 cut(s) 327, 492
BseMI GCAATG 1 cut(s) 258
BseMII CTCAG 4 cut(s) 143, 447, 539, 638
BseNI ACTGG 1 cut(s) 321
BseRI GAGGAG 3 cut(s) 20, 31, 57
BseXI GCAGC 4 cut(s) 204, 379, 875, 878
Bsh1236I CGCG 1 cut(s) 63
BshFI GGCC 2 cut(s) 8, 599
BsiHKAI GWGCWC 1 cut(s) 311
BslI CCNNNNNNNGG 2 cut(s) 327, 492
BsmAI GTCTC 3 cut(s) 94, 187, 452
BsmBI CGTCTC 2 cut(s) 94, 187
BsnI GGCC 2 cut(s) 8, 599
Bsp1286I GDGCHC 1 cut(s) 311
Bsp143I GATC 5 cut(s) 132, 223, 561, 616, 640
Bsp19I CCATGG 2 cut(s) 9, 486
BspACI CCGC 5 cut(s) 58, 61, 201, 411, 441
BspANI GGCC 2 cut(s) 8, 599
BspCNI CTCAG 4 cut(s) 142, 448, 538, 637
BspFNI CGCG 1 cut(s) 63
BspPI GGATC 2 cut(s) 218, 569
BspQI GCTCTTC 1 cut(s) 299
BspTI CTTAAG 1 cut(s) 682
BsrBI CCGCTC 1 cut(s) 441
BsrDI GCAATG 1 cut(s) 258
BsrI ACTGG 1 cut(s) 321
BssECI CCNNGG 4 cut(s) 9, 207, 486, 600
BssMI GATC 5 cut(s) 132, 223, 561, 616, 640
BssT1I CCWWGG 3 cut(s) 9, 207, 486
Bst2UI CCWGG 1 cut(s) 601
Bst4CI ACNGT 2 cut(s) 371, 736
Bst6I CTCTTC 2 cut(s) 299, 693
BstAFI CTTAAG 1 cut(s) 682
BstC8I GCNNGC 1 cut(s) 397
BstDEI CTNAG 4 cut(s) 129, 456, 525, 624
BstDSI CCRYGG 2 cut(s) 9, 486
BstF5I GGATG 1 cut(s) 482
BstFNI CGCG 1 cut(s) 63
BstH2I RGCGCY 1 cut(s) 198
BstHHI GCGC 2 cut(s) 65, 197
BstKTI GATC 5 cut(s) 135, 226, 564, 619, 643
BstMAI GTCTC 3 cut(s) 94, 187, 452
BstMBI GATC 5 cut(s) 132, 223, 561, 616, 640
BstNI CCWGG 1 cut(s) 601
BstSCI CCNGG 1 cut(s) 599
BstSFI CTRYAG 1 cut(s) 216
BstUI CGCG 1 cut(s) 63
BstV1I GCAGC 4 cut(s) 204, 379, 875, 878
BstV2I GAAGAC 1 cut(s) 804
BstX2I RGATCY 1 cut(s) 132
BstXI CCANNNNNNTGG 1 cut(s) 607
BstYI RGATCY 1 cut(s) 132
BsuRI GGCC 2 cut(s) 8, 599
BtgI CCRYGG 2 cut(s) 9, 486
BtrI CACGTC 3 cut(s) 88, 259, 580
BtsCI GGATG 1 cut(s) 482
BtsIMutI CAGTG 1 cut(s) 376
Cac8I GCNNGC 1 cut(s) 397
CfoI GCGC 2 cut(s) 65, 197
Csp6I GTAC 1 cut(s) 687
CviAII CATG 4 cut(s) 10, 487, 557, 802
CviJI RGCY 8 cut(s) 8, 309, 378, 392, 599, 628, 725, 755
CviKI_1 RGCY 8 cut(s) 8, 309, 378, 392, 599, 628, 725, 755
CviQI GTAC 1 cut(s) 687
DdeI CTNAG 4 cut(s) 129, 456, 525, 624
DpnI GATC 5 cut(s) 134, 225, 563, 618, 642
DpnII GATC 5 cut(s) 132, 223, 561, 616, 640
EaeI YGGCCR 2 cut(s) 6, 597
Eam1104I CTCTTC 2 cut(s) 299, 693
EarI CTCTTC 2 cut(s) 299, 693
EciI GGCGGA 1 cut(s) 47
Ecl136II GAGCTC 1 cut(s) 309
Eco130I CCWWGG 3 cut(s) 9, 207, 486
Eco24I GRGCYC 1 cut(s) 311
Eco47III AGCGCT 1 cut(s) 196
Eco53kI GAGCTC 1 cut(s) 309
Eco57I CTGAAG 1 cut(s) 483
EcoICRI GAGCTC 1 cut(s) 309
EcoRII CCWGG 1 cut(s) 599
EcoT14I CCWWGG 3 cut(s) 9, 207, 486
EcoT38I GRGCYC 1 cut(s) 311
ErhI CCWWGG 3 cut(s) 9, 207, 486
Esp3I CGTCTC 2 cut(s) 94, 187
FaeI CATG 4 cut(s) 13, 490, 560, 805
FatI CATG 4 cut(s) 9, 486, 556, 801
FauNDI CATATG 1 cut(s) 694
Fnu4HI GCNGC 6 cut(s) 61, 193, 393, 411, 864, 867
FokI GGATG 1 cut(s) 469
FriOI GRGCYC 1 cut(s) 311
Fsp4HI GCNGC 6 cut(s) 61, 193, 393, 411, 864, 867
FspBI CTAG 1 cut(s) 840
GlaI GCGC 2 cut(s) 64, 196
GluI GCNGC 6 cut(s) 61, 193, 393, 411, 864, 867
GsuI CTGGAG 2 cut(s) 531, 830
HaeII RGCGCY 1 cut(s) 198
HaeIII GGCC 2 cut(s) 8, 599
HhaI GCGC 2 cut(s) 65, 197
Hin1II CATG 4 cut(s) 13, 490, 560, 805
Hin6I GCGC 2 cut(s) 63, 195
HinP1I GCGC 2 cut(s) 63, 195
HincII GTYRAC 1 cut(s) 433
HindII GTYRAC 1 cut(s) 433
HinfI GANTC 3 cut(s) 25, 452, 676
HphI GGTGA 1 cut(s) 255
Hpy166II GTNNAC 2 cut(s) 170, 433
Hpy188I TCNGA 5 cut(s) 132, 187, 299, 457, 528
Hpy188III TCNNGA 3 cut(s) 233, 665, 809
Hpy8I GTNNAC 2 cut(s) 170, 433
Hpy99I CGWCG 1 cut(s) 100
HpyAV CCTTC 3 cut(s) 55, 131, 241
HpyCH4III ACNGT 2 cut(s) 371, 736
HpyCH4IV ACGT 3 cut(s) 87, 258, 579
HpyCH4V TGCA 5 cut(s) 521, 635, 692, 719, 731
HpyF3I CTNAG 4 cut(s) 129, 456, 525, 624
HpySE526I ACGT 3 cut(s) 87, 258, 579
Hsp92II CATG 4 cut(s) 13, 490, 560, 805
HspAI GCGC 2 cut(s) 63, 195
Kzo9I GATC 5 cut(s) 132, 223, 561, 616, 640
LguI GCTCTTC 1 cut(s) 299
LmnI GCTCC 2 cut(s) 70, 314
Lsp1109I GCAGC 4 cut(s) 204, 379, 875, 878
LweI GCATC 1 cut(s) 718
MaeI CTAG 1 cut(s) 840
MaeII ACGT 3 cut(s) 87, 258, 579
MaeIII GTNAC 3 cut(s) 261, 348, 787
MalI GATC 5 cut(s) 134, 225, 563, 618, 642
MbiI CCGCTC 1 cut(s) 441
MboI GATC 5 cut(s) 132, 223, 561, 616, 640
MboII GAAGA 8 cut(s) 253, 256, 316, 626, 710, 809, 859, 862
MflI RGATCY 1 cut(s) 132
MhlI GDGCHC 1 cut(s) 311
MlsI TGGCCA 2 cut(s) 8, 599
MluCI AATT 3 cut(s) 332, 476, 777
MluNI TGGCCA 2 cut(s) 8, 599
MlyI GAGTC 3 cut(s) 19, 461, 670
MmeI TCCRAC 1 cut(s) 277
Mox20I TGGCCA 2 cut(s) 8, 599
MscI TGGCCA 2 cut(s) 8, 599
MseI TTAA 3 cut(s) 551, 683, 880
Msp20I TGGCCA 2 cut(s) 8, 599
MspCI CTTAAG 1 cut(s) 682
MspR9I CCNGG 1 cut(s) 601
MvaI CCWGG 1 cut(s) 601
MvnI CGCG 1 cut(s) 63
NcoI CCATGG 2 cut(s) 9, 486
NdeI CATATG 1 cut(s) 694
NdeII GATC 5 cut(s) 132, 223, 561, 616, 640
NlaIII CATG 4 cut(s) 13, 490, 560, 805
NmuCI GTSAC 2 cut(s) 261, 787
PciSI GCTCTTC 1 cut(s) 299
PcsI WCGNNNNNNNCGW 1 cut(s) 183
PkrI GCNGC 6 cut(s) 62, 194, 394, 412, 865, 868
PleI GAGTC 3 cut(s) 19, 460, 670
PpsI GAGTC 3 cut(s) 19, 460, 670
Psp124BI GAGCTC 1 cut(s) 311
Psp6I CCWGG 1 cut(s) 599
PspGI CCWGG 1 cut(s) 599
PsuI RGATCY 1 cut(s) 132
RsaI GTAC 1 cut(s) 688
RsaNI GTAC 1 cut(s) 687
SacI GAGCTC 1 cut(s) 311
SapI GCTCTTC 1 cut(s) 299
SaqAI TTAA 3 cut(s) 551, 683, 880
SatI GCNGC 6 cut(s) 61, 193, 393, 411, 864, 867
Sau3AI GATC 5 cut(s) 132, 223, 561, 616, 640
ScaI AGTACT 1 cut(s) 688
SchI GAGTC 3 cut(s) 19, 461, 670
ScrFI CCNGG 1 cut(s) 601
SduI GDGCHC 1 cut(s) 311
SfaNI GCATC 1 cut(s) 718
SfcI CTRYAG 1 cut(s) 216
SmlI CTYRAG 2 cut(s) 682, 818
SmoI CTYRAG 2 cut(s) 682, 818
Sse9I AATT 3 cut(s) 332, 476, 777
SsiI CCGC 5 cut(s) 58, 61, 201, 411, 441
SspMI CTAG 1 cut(s) 840
SstI GAGCTC 1 cut(s) 311
StyD4I CCNGG 1 cut(s) 599
StyI CCWWGG 3 cut(s) 9, 207, 486
TaaI ACNGT 2 cut(s) 371, 736
TaiI ACGT 3 cut(s) 90, 261, 582
TaqI TCGA 3 cut(s) 98, 366, 612
TasI AATT 3 cut(s) 332, 476, 777
TatI WGTACW 1 cut(s) 686
TauI GCSGC 2 cut(s) 63, 413
Tru1I TTAA 3 cut(s) 551, 683, 880
Tru9I TTAA 3 cut(s) 551, 683, 880
TscAI CASTG 1 cut(s) 376
TseFI GTSAC 2 cut(s) 261, 787
TseI GCWGC 4 cut(s) 192, 392, 863, 866
Tsp45I GTSAC 2 cut(s) 261, 787
TspDTI ATGAA 3 cut(s) 317, 711, 789
TspGWI ACGGA 2 cut(s) 60, 155
TspRI CASTG 1 cut(s) 376
Vha464I CTTAAG 1 cut(s) 682
XapI RAATTY 1 cut(s) 332
XcmI CCANNNNNNNNNTGG 1 cut(s) 799
XspI CTAG 1 cut(s) 840
ZrmI AGTACT 1 cut(s) 688
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.