MD09G1006100.v1.1
NAC Family

inactive shikimate kinase like 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0013212
Physical Location & Seq
Reverse (-)
452446 .. 454737
2292 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1006100.v1.1.491

Sequence Viewer

Length: 861 bp
ATGGAGATAACAAGGAGCCTCCTACTCCATTCGTCTTGCTCCAAGCTCCCACTCCGCACTCCCTTCCGCCACTCTCAAGTTCCGGCTTCCACTTTCTCAATTCCGACTACGTCTCGCCCCCACTTCCGAAAACCTCTCGCCGCCTCACGCTCCGCCTCCGACGGCGGCGATTCAAGCTCAGCAGCCAAGGTTTCTGTAGCTGACCCGTCTACGGCAGTGAAGAACAAGGCAATAGATATAACCCCTGAGCTGAAGGGAACTTCCATATTTCTTGTGGGCATGAAGAGCTCCATGAAAACCAGTTTGGGGAAGCTCCTAGCCAATGTGTTGCGGTATTATTACTTTGACAGTGATAGTTTGGTTGAGGAAGCTGCTGGCGGTGGATCTGCTGCCAAATTGCTTAGGGAGGCTGACACAAATGGTTATCGGGAATCTGAGACTGAAGTATTGAAGCAATTATCATCCATGGGTCGATTGGTGGTTTGTGCTGGAGATGGTGCAGTTCAGAGTTCAACTAATCTGGCTCTTCTGAGATATGGAATCTCTATATGGATTGATGTACCTCTAGACTTGGTGGCCAGGGGCATGATTGAAGATCAAAGTCAGCTTCCTGCACTCAACGTATCTGCTTCAGCATCTTATCCAGAGGTTTTGACTCACCTGAGTACTATGTATGAAGAAGTCAGAGGTGGATATGAAACAGCTGATGCAACCGTTTCCATTGAAAAACTAGCTTATCAATTAGGGTATGATGATTTTGGTGATGTAACCACAGAAGACGTGGCATTGGAGGTTCTGAAGGAGATGGAGAAACTGACCAGAGTAAAGAAGATGATGGAAGCGGCGGCAAGACCTTTTTGA

Protein Analysis

287

Amino Acids

30.89

Weight (kDa)

5.69

Isoelectric Point (pI)

43.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SKI PF01202 98 - 228 2.2e-23 Shikimate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013212)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26900 AT3G26900 AT3G26900
fragaria_vesca FvH4_6g54030 FvH4_6g54030
malus_domestica MD09G1006100.v1.1 MD17G1010100.v1.1
prunus_persica Prupe.3G310900_v2.0.a1
pyrus_communis pycom17g00460
rosa_chinensis RchiOBHm_Chr2g0176211
rosa_laevigata RLG00000022390
rosa_multiflora Rmu_co8135912.1_g000001
rosa_rugosa Rorug02G0591800
rosa_samantha Rh2AG670400 Rh2BG681900 Rh2CG644700 Rh2DG695500
rosa_wichuraiana Rw2G054990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 209
AciI CCGC 9 cut(s) 55, 67, 141, 153, 165, 331, 378, 842, 845
AclWI GGATC 1 cut(s) 391
AcoI YGGCCR 1 cut(s) 576
AcuI CTGAAG 4 cut(s) 272, 462, 615, 818
AfaI GTAC 2 cut(s) 561, 667
AfiI CCNNNNNNNGG 2 cut(s) 211, 306
AgsI TTSAA 5 cut(s) 174, 451, 513, 593, 725
AjiI CACGTC 1 cut(s) 781
AjnI CCWGG 1 cut(s) 578
AjuI GAANNNNNNNTTGG 2 cut(s) 287, 319
Alw21I GWGCWC 1 cut(s) 290
Alw26I GTCTC 2 cut(s) 117, 431
AlwI GGATC 1 cut(s) 391
AoxI GGCC 1 cut(s) 576
ApeKI GCWGC 3 cut(s) 182, 371, 389
AsuHPI GGTGA 2 cut(s) 650, 773
BalI TGGCCA 1 cut(s) 578
BanII GRGCYC 1 cut(s) 290
BbsI GAAGAC 1 cut(s) 783
Bbv12I GWGCWC 1 cut(s) 290
BbvI GCAGC 3 cut(s) 194, 358, 376
BccI CCATC 3 cut(s) 488, 799, 829
BceAI ACGGC 2 cut(s) 178, 228
BciT130I CCWGG 1 cut(s) 580
BcoDI GTCTC 2 cut(s) 117, 431
BfaI CTAG 3 cut(s) 317, 566, 731
BfmI CTRYAG 1 cut(s) 195
BisI GCNGC 7 cut(s) 141, 166, 183, 372, 390, 843, 846
BlpI GCTNAGC 1 cut(s) 178
BlsI GCNGC 7 cut(s) 142, 167, 184, 373, 391, 844, 847
BmcAI AGTACT 1 cut(s) 667
Bme1390I CCNGG 1 cut(s) 580
BmgBI CACGTC 1 cut(s) 781
BmiI GGNNCC 1 cut(s) 17
BmrFI CCNGG 1 cut(s) 580
BmsI GCATC 2 cut(s) 644, 697
BpiI GAAGAC 1 cut(s) 783
BpmI CTGGAG 1 cut(s) 510
Bpu10I CCTNAGC 2 cut(s) 246, 401
Bpu1102I GCTNAGC 1 cut(s) 178
BpuEI CTTGAG 1 cut(s) 60
BsaJI CCNNGG 3 cut(s) 186, 465, 579
Bsc4I CCNNNNNNNGG 2 cut(s) 211, 306
Bse1I ACTGG 1 cut(s) 300
BseBI CCWGG 1 cut(s) 580
BseDI CCNNGG 3 cut(s) 186, 465, 579
BseGI GGATG 1 cut(s) 461
BseLI CCNNNNNNNGG 2 cut(s) 211, 306
BseMII CTCAG 5 cut(s) 192, 237, 426, 521, 653
BseNI ACTGG 1 cut(s) 300
BseXI GCAGC 3 cut(s) 194, 358, 376
BsgI GTGCAG 2 cut(s) 519, 597
BshFI GGCC 1 cut(s) 578
BsiHKAI GWGCWC 1 cut(s) 290
BsiSI CCGG 1 cut(s) 83
BslI CCNNNNNNNGG 2 cut(s) 211, 306
BsmAI GTCTC 2 cut(s) 117, 431
BsmBI CGTCTC 1 cut(s) 117
BsnI GGCC 1 cut(s) 578
Bsp1286I GDGCHC 1 cut(s) 290
Bsp143I GATC 2 cut(s) 383, 595
Bsp1720I GCTNAGC 1 cut(s) 178
Bsp19I CCATGG 1 cut(s) 465
BspACI CCGC 9 cut(s) 55, 67, 141, 153, 165, 331, 378, 842, 845
BspANI GGCC 1 cut(s) 578
BspCNI CTCAG 5 cut(s) 191, 238, 427, 522, 654
BspLI GGNNCC 1 cut(s) 17
BspPI GGATC 1 cut(s) 391
BspQI GCTCTTC 2 cut(s) 278, 531
BsrI ACTGG 1 cut(s) 300
BssECI CCNNGG 3 cut(s) 186, 465, 579
BssMI GATC 2 cut(s) 383, 595
BssT1I CCWWGG 2 cut(s) 186, 465
Bst2UI CCWGG 1 cut(s) 580
Bst4CI ACNGT 2 cut(s) 350, 715
Bst6I CTCTTC 2 cut(s) 278, 531
BstC8I GCNNGC 1 cut(s) 376
BstDEI CTNAG 6 cut(s) 178, 246, 401, 435, 530, 662
BstDSI CCRYGG 1 cut(s) 465
BstF5I GGATG 1 cut(s) 461
BstKTI GATC 2 cut(s) 386, 598
BstMAI GTCTC 2 cut(s) 117, 431
BstMBI GATC 2 cut(s) 383, 595
BstMWI GCNNNNNNNGC 2 cut(s) 174, 285
BstNI CCWGG 1 cut(s) 580
BstSCI CCNGG 1 cut(s) 578
BstSFI CTRYAG 1 cut(s) 195
BstV1I GCAGC 3 cut(s) 194, 358, 376
BstV2I GAAGAC 1 cut(s) 783
BstX2I RGATCY 1 cut(s) 383
BstYI RGATCY 1 cut(s) 383
BsuRI GGCC 1 cut(s) 578
BtgI CCRYGG 1 cut(s) 465
BtrI CACGTC 1 cut(s) 781
BtsCI GGATG 1 cut(s) 461
BtsI GCAGTG 1 cut(s) 222
BtsIMutI CAGTG 2 cut(s) 222, 355
Cac8I GCNNGC 1 cut(s) 376
Csp6I GTAC 2 cut(s) 560, 666
CviAII CATG 4 cut(s) 280, 292, 466, 586
CviQI GTAC 2 cut(s) 560, 666
DdeI CTNAG 6 cut(s) 178, 246, 401, 435, 530, 662
DpnI GATC 2 cut(s) 385, 597
DpnII GATC 2 cut(s) 383, 595
EaeI YGGCCR 1 cut(s) 576
Eam1104I CTCTTC 2 cut(s) 278, 531
EarI CTCTTC 2 cut(s) 278, 531
EciI GGCGGA 2 cut(s) 56, 142
Ecl136II GAGCTC 1 cut(s) 288
Eco130I CCWWGG 2 cut(s) 186, 465
Eco24I GRGCYC 1 cut(s) 290
Eco53kI GAGCTC 1 cut(s) 288
Eco57I CTGAAG 4 cut(s) 272, 462, 615, 818
EcoICRI GAGCTC 1 cut(s) 288
EcoRII CCWGG 1 cut(s) 578
EcoT14I CCWWGG 2 cut(s) 186, 465
EcoT38I GRGCYC 1 cut(s) 290
ErhI CCWWGG 2 cut(s) 186, 465
Esp3I CGTCTC 1 cut(s) 117
FaeI CATG 4 cut(s) 283, 295, 469, 589
FatI CATG 4 cut(s) 279, 291, 465, 585
FblI GTMKAC 1 cut(s) 209
Fnu4HI GCNGC 7 cut(s) 141, 166, 183, 372, 390, 843, 846
FokI GGATG 1 cut(s) 448
FriOI GRGCYC 1 cut(s) 290
Fsp4HI GCNGC 7 cut(s) 141, 166, 183, 372, 390, 843, 846
FspBI CTAG 3 cut(s) 317, 566, 731
GluI GCNGC 7 cut(s) 141, 166, 183, 372, 390, 843, 846
GsuI CTGGAG 1 cut(s) 510
HaeIII GGCC 1 cut(s) 578
HapII CCGG 1 cut(s) 83
Hin1II CATG 4 cut(s) 283, 295, 469, 589
HinfI GANTC 4 cut(s) 170, 431, 540, 655
HpaII CCGG 1 cut(s) 83
HphI GGTGA 2 cut(s) 650, 773
Hpy166II GTNNAC 1 cut(s) 210
Hpy188I TCNGA 8 cut(s) 105, 128, 160, 436, 507, 531, 686, 798
Hpy188III TCNNGA 3 cut(s) 428, 566, 644
Hpy8I GTNNAC 1 cut(s) 210
Hpy99I CGWCG 1 cut(s) 164
HpyAV CCTTC 3 cut(s) 73, 247, 793
HpyCH4III ACNGT 2 cut(s) 350, 715
HpyCH4IV ACGT 3 cut(s) 110, 621, 780
HpyCH4V TGCA 3 cut(s) 500, 614, 710
HpyF10VI GCNNNNNNNGC 2 cut(s) 174, 285
HpyF3I CTNAG 6 cut(s) 178, 246, 401, 435, 530, 662
HpySE526I ACGT 3 cut(s) 110, 621, 780
Hsp92II CATG 4 cut(s) 283, 295, 469, 589
Kzo9I GATC 2 cut(s) 383, 595
LguI GCTCTTC 2 cut(s) 278, 531
LmnI GCTCC 6 cut(s) 15, 44, 51, 155, 293, 318
Lsp1109I GCAGC 3 cut(s) 194, 358, 376
LweI GCATC 2 cut(s) 644, 697
MaeI CTAG 3 cut(s) 317, 566, 731
MaeII ACGT 3 cut(s) 110, 621, 780
MaeIII GTNAC 1 cut(s) 766
MalI GATC 2 cut(s) 385, 597
MboI GATC 2 cut(s) 383, 595
MboII GAAGA 7 cut(s) 232, 295, 518, 605, 689, 788, 841
MflI RGATCY 1 cut(s) 383
MhlI GDGCHC 1 cut(s) 290
MlsI TGGCCA 1 cut(s) 578
MluCI AATT 4 cut(s) 99, 395, 455, 740
MluNI TGGCCA 1 cut(s) 578
MlyI GAGTC 1 cut(s) 649
MmeI TCCRAC 2 cut(s) 128, 183
Mox20I TGGCCA 1 cut(s) 578
MscI TGGCCA 1 cut(s) 578
Msp20I TGGCCA 1 cut(s) 578
MspA1I CMGCKG 1 cut(s) 704
MspI CCGG 1 cut(s) 83
MspR9I CCNGG 1 cut(s) 580
MvaI CCWGG 1 cut(s) 580
MwoI GCNNNNNNNGC 2 cut(s) 174, 285
NcoI CCATGG 1 cut(s) 465
NdeII GATC 2 cut(s) 383, 595
NlaIII CATG 4 cut(s) 283, 295, 469, 589
NlaIV GGNNCC 1 cut(s) 17
PciSI GCTCTTC 2 cut(s) 278, 531
PfeI GAWTC 3 cut(s) 170, 431, 540
PflFI GACNNNGTC 1 cut(s) 109
PkrI GCNGC 7 cut(s) 142, 167, 184, 373, 391, 844, 847
PleI GAGTC 1 cut(s) 649
PpsI GAGTC 1 cut(s) 649
Psp124BI GAGCTC 1 cut(s) 290
Psp6I CCWGG 1 cut(s) 578
PspGI CCWGG 1 cut(s) 578
PspN4I GGNNCC 1 cut(s) 17
PsuI RGATCY 1 cut(s) 383
PsyI GACNNNGTC 1 cut(s) 109
PvuII CAGCTG 1 cut(s) 704
RsaI GTAC 2 cut(s) 561, 667
RsaNI GTAC 2 cut(s) 560, 666
SacI GAGCTC 1 cut(s) 290
SapI GCTCTTC 2 cut(s) 278, 531
SatI GCNGC 7 cut(s) 141, 166, 183, 372, 390, 843, 846
Sau3AI GATC 2 cut(s) 383, 595
ScaI AGTACT 1 cut(s) 667
SchI GAGTC 1 cut(s) 649
ScrFI CCNGG 1 cut(s) 580
SduI GDGCHC 1 cut(s) 290
SfaNI GCATC 2 cut(s) 644, 697
SfcI CTRYAG 1 cut(s) 195
SmlI CTYRAG 1 cut(s) 75
SmoI CTYRAG 1 cut(s) 75
Sse9I AATT 4 cut(s) 99, 395, 455, 740
SsiI CCGC 9 cut(s) 55, 67, 141, 153, 165, 331, 378, 842, 845
SspMI CTAG 3 cut(s) 317, 566, 731
SstI GAGCTC 1 cut(s) 290
StyD4I CCNGG 1 cut(s) 578
StyI CCWWGG 2 cut(s) 186, 465
TaaI ACNGT 2 cut(s) 350, 715
TaiI ACGT 3 cut(s) 113, 624, 783
TaqI TCGA 1 cut(s) 472
TasI AATT 4 cut(s) 99, 395, 455, 740
TatI WGTACW 1 cut(s) 665
TauI GCSGC 4 cut(s) 143, 168, 845, 848
TfiI GAWTC 3 cut(s) 170, 431, 540
TscAI CASTG 2 cut(s) 222, 355
TseI GCWGC 3 cut(s) 182, 371, 389
TspDTI ATGAA 4 cut(s) 296, 308, 690, 711
TspRI CASTG 2 cut(s) 222, 355
Tth111I GACNNNGTC 1 cut(s) 109
XbaI TCTAGA 1 cut(s) 565
XcmI CCANNNNNNNNNTGG 3 cut(s) 271, 472, 778
XmiI GTMKAC 1 cut(s) 209
XspI CTAG 3 cut(s) 317, 566, 731
ZrmI AGTACT 1 cut(s) 667
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.