RLG00000022390
NAC Family

inactive shikimate kinase like 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0013212
Physical Location & Seq
Forward (+)
83056302 .. 83058499
2198 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022390

Sequence Viewer

Length: 879 bp
ATGGAGATAACAAGGACTCTCCTCCCTTCCTCCTCTTGCTCTAGTCTCCACCTCCGCACTCCGGTCGTCCATTTTCACGTCTCGCCTTCGCACTCACAGTCGTTACCCACGGATGTCCTTCAGTCTCAGACCTCACTGCCCATTCCATCTCGCACCCGCTTCCGGAAACCTCTCGCCGCCTCCGATGGCACCACTTCCGCAACTAAGGTTGCTGTGGATGATCCCTCTCTTGCTGTGAAGAAGAAGGCAATGGATGTATCACCAGAACTCAAAGGAACTACCATATTCCTTGTGGGCATGAAGAGCTACATAAAGACCAGTTTGGGGAAATTCCTCGCCAACGTGTTACGATACTATTACTTTGACAGTGATAGTTTGGTTGAGGAAGCTGCTGGCACTCAATCTGCCACCAAATCTTTAAGGGAGACTGACAAGAGTGGTTTCCAGGAGTCCGAGACTGAAGTGTTGAAGCAATTATCATCCATGGGTCGGTTAGTGGTTTGTGCTGGAGATGGTGCAACTCAGAGTTCAACAAATCTGGCTCTTTTAAGACATGGGATCACGATATGGATTGACGTACCTTTAGACATTGTGGCCAGGAGTGTGGTCGAAGATCAAACTCAGCTCTCTGCATACAACCTATCTACTTCCGTATCCTATCCGGAGGTTTTGACTCACCTAAGTACTTCATATGAAGAGTTGAGAGGTGGATATGCAATAGCTGATGCAACCGTATCTCTTCAGCAAGTAGCTGGTAAGTTAGGTTATGATGAAATCGATGATGTAACCACCGAAGACATGGCTTTGGAGGTTCTCAAGGAGGTAGAGAAATTGACTAGAGTGAAGAAGATGATGGAAGCAGCAGCAAGACCTTTTTAA

Protein Analysis

293

Amino Acids

31.8

Weight (kDa)

5.74

Isoelectric Point (pI)

47.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SKI PF01202 105 - 225 2.6e-23 Shikimate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013212)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26900 AT3G26900 AT3G26900
fragaria_vesca FvH4_6g54030 FvH4_6g54030
malus_domestica MD09G1006100.v1.1 MD17G1010100.v1.1
prunus_persica Prupe.3G310900_v2.0.a1
pyrus_communis pycom17g00460
rosa_chinensis RchiOBHm_Chr2g0176211
rosa_laevigata RLG00000022390
rosa_multiflora Rmu_co8135912.1_g000001
rosa_rugosa Rorug02G0591800
rosa_samantha Rh2AG670400 Rh2BG681900 Rh2CG644700 Rh2DG695500
rosa_wichuraiana Rw2G054990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 188
AccIII TCCGGA 2 cut(s) 162, 661
AciI CCGC 4 cut(s) 55, 157, 177, 198
AclWI GGATC 2 cut(s) 215, 566
AcoI YGGCCR 1 cut(s) 594
AcsI RAATTY 1 cut(s) 329
AcuI CTGAAG 3 cut(s) 104, 480, 725
AfaI GTAC 2 cut(s) 579, 685
AfiI CCNNNNNNNGG 4 cut(s) 61, 162, 324, 489
AflIII ACRYGT 1 cut(s) 342
AgsI TTSAA 2 cut(s) 469, 531
AjiI CACGTC 1 cut(s) 79
AjnI CCWGG 2 cut(s) 444, 596
AluBI AGCT 5 cut(s) 306, 389, 625, 722, 752
AluI AGCT 5 cut(s) 306, 389, 625, 722, 752
Alw26I GTCTC 5 cut(s) 50, 85, 129, 419, 449
AlwI GGATC 2 cut(s) 215, 566
Aor13HI TCCGGA 2 cut(s) 162, 661
AoxI GGCC 1 cut(s) 594
ApeKI GCWGC 3 cut(s) 389, 860, 863
ApoI RAATTY 1 cut(s) 329
AsuHPI GGTGA 2 cut(s) 252, 668
BaeI ACNNNNGTAYC 2 cut(s) 636, 669
BalI TGGCCA 1 cut(s) 596
BanI GGYRCC 1 cut(s) 188
BbsI GAAGAC 1 cut(s) 801
BbvI GCAGC 3 cut(s) 376, 872, 875
BccI CCATC 4 cut(s) 154, 179, 506, 847
BcgI CGANNNNNNTGC 2 cut(s) 46, 80
BciT130I CCWGG 2 cut(s) 446, 598
BciVI GTATCC 1 cut(s) 664
BcoDI GTCTC 5 cut(s) 50, 85, 129, 419, 449
BfaI CTAG 2 cut(s) 42, 837
BfuI GTATCC 1 cut(s) 664
BisI GCNGC 4 cut(s) 177, 390, 861, 864
BlsI GCNGC 4 cut(s) 178, 391, 862, 865
BmcAI AGTACT 1 cut(s) 685
Bme1390I CCNGG 2 cut(s) 446, 598
BmgBI CACGTC 1 cut(s) 79
BmiI GGNNCC 1 cut(s) 190
BmrFI CCNGG 2 cut(s) 446, 598
BmsI GCATC 1 cut(s) 715
BpiI GAAGAC 1 cut(s) 801
BpmI CTGGAG 1 cut(s) 528
BpuEI CTTGAG 1 cut(s) 800
Bsa29I ATCGAT 1 cut(s) 777
BsaJI CCNNGG 2 cut(s) 108, 483
BsaWI WCCGGW 3 cut(s) 61, 162, 661
Bsc4I CCNNNNNNNGG 4 cut(s) 61, 162, 324, 489
Bse1I ACTGG 1 cut(s) 318
Bse3DI GCAATG 1 cut(s) 255
BseAI TCCGGA 2 cut(s) 162, 661
BseBI CCWGG 2 cut(s) 446, 598
BseCI ATCGAT 1 cut(s) 777
BseDI CCNNGG 2 cut(s) 108, 483
BseGI GGATG 4 cut(s) 118, 223, 259, 479
BseLI CCNNNNNNNGG 4 cut(s) 61, 162, 324, 489
BseMI GCAATG 1 cut(s) 255
BseMII CTCAG 3 cut(s) 140, 536, 635
BseNI ACTGG 1 cut(s) 318
BseRI GAGGAG 2 cut(s) 11, 22
BseXI GCAGC 3 cut(s) 376, 872, 875
Bsh1285I CGRYCG 1 cut(s) 66
BshFI GGCC 1 cut(s) 596
BshNI GGYRCC 1 cut(s) 188
BshVI ATCGAT 1 cut(s) 777
BsiEI CGRYCG 1 cut(s) 66
BsiSI CCGG 3 cut(s) 62, 163, 662
BslI CCNNNNNNNGG 4 cut(s) 61, 162, 324, 489
BsmAI GTCTC 5 cut(s) 50, 85, 129, 419, 449
BsmBI CGTCTC 1 cut(s) 85
BsnI GGCC 1 cut(s) 596
Bsp13I TCCGGA 2 cut(s) 162, 661
Bsp143I GATC 3 cut(s) 220, 558, 613
Bsp19I CCATGG 1 cut(s) 483
BspACI CCGC 4 cut(s) 55, 157, 177, 198
BspANI GGCC 1 cut(s) 596
BspCNI CTCAG 3 cut(s) 139, 535, 634
BspDI ATCGAT 1 cut(s) 777
BspEI TCCGGA 2 cut(s) 162, 661
BspLI GGNNCC 1 cut(s) 190
BspPI GGATC 2 cut(s) 215, 566
BspQI GCTCTTC 1 cut(s) 296
BspT107I GGYRCC 1 cut(s) 188
BsrDI GCAATG 1 cut(s) 255
BsrI ACTGG 1 cut(s) 318
BssECI CCNNGG 2 cut(s) 108, 483
BssMI GATC 3 cut(s) 220, 558, 613
BssT1I CCWWGG 1 cut(s) 483
Bst2UI CCWGG 2 cut(s) 446, 598
Bst4CI ACNGT 3 cut(s) 99, 368, 733
Bst6I CTCTTC 3 cut(s) 296, 690, 744
BstC8I GCNNGC 1 cut(s) 394
BstDEI CTNAG 5 cut(s) 126, 204, 522, 621, 680
BstDSI CCRYGG 2 cut(s) 108, 483
BstF5I GGATG 4 cut(s) 118, 223, 259, 479
BstKTI GATC 3 cut(s) 223, 561, 616
BstMAI GTCTC 5 cut(s) 50, 85, 129, 419, 449
BstMBI GATC 3 cut(s) 220, 558, 613
BstMCI CGRYCG 1 cut(s) 66
BstMWI GCNNNNNNNGC 1 cut(s) 303
BstNI CCWGG 2 cut(s) 446, 598
BstSCI CCNGG 2 cut(s) 444, 596
BstV1I GCAGC 3 cut(s) 376, 872, 875
BstV2I GAAGAC 1 cut(s) 801
BstXI CCANNNNNNTGG 1 cut(s) 604
Bsu15I ATCGAT 1 cut(s) 777
BsuI GTATCC 1 cut(s) 664
BsuRI GGCC 1 cut(s) 596
BsuTUI ATCGAT 1 cut(s) 777
BtgI CCRYGG 2 cut(s) 108, 483
BtrI CACGTC 1 cut(s) 79
BtsCI GGATG 4 cut(s) 118, 223, 259, 479
BtsI GCAGTG 1 cut(s) 134
BtsIMutI CAGTG 2 cut(s) 134, 373
Cac8I GCNNGC 1 cut(s) 394
ClaI ATCGAT 1 cut(s) 777
Csp6I GTAC 2 cut(s) 578, 684
CviAII CATG 4 cut(s) 298, 484, 554, 799
CviJI RGCY 8 cut(s) 306, 389, 542, 596, 625, 722, 752, 803
CviKI_1 RGCY 8 cut(s) 306, 389, 542, 596, 625, 722, 752, 803
CviQI GTAC 2 cut(s) 578, 684
DdeI CTNAG 5 cut(s) 126, 204, 522, 621, 680
DpnI GATC 3 cut(s) 222, 560, 615
DpnII GATC 3 cut(s) 220, 558, 613
EaeI YGGCCR 1 cut(s) 594
Eam1104I CTCTTC 3 cut(s) 296, 690, 744
EarI CTCTTC 3 cut(s) 296, 690, 744
Eco130I CCWWGG 1 cut(s) 483
Eco57I CTGAAG 3 cut(s) 104, 480, 725
EcoRII CCWGG 2 cut(s) 444, 596
EcoT14I CCWWGG 1 cut(s) 483
ErhI CCWWGG 1 cut(s) 483
Esp3I CGTCTC 1 cut(s) 85
FaeI CATG 4 cut(s) 301, 487, 557, 802
FatI CATG 4 cut(s) 297, 483, 553, 798
FauI CCCGC 1 cut(s) 164
FauNDI CATATG 1 cut(s) 691
Fnu4HI GCNGC 4 cut(s) 177, 390, 861, 864
FokI GGATG 4 cut(s) 125, 230, 266, 466
Fsp4HI GCNGC 4 cut(s) 177, 390, 861, 864
FspBI CTAG 2 cut(s) 42, 837
GluI GCNGC 4 cut(s) 177, 390, 861, 864
GsuI CTGGAG 1 cut(s) 528
HaeIII GGCC 1 cut(s) 596
HapII CCGG 3 cut(s) 62, 163, 662
Hin1II CATG 4 cut(s) 301, 487, 557, 802
HinfI GANTC 3 cut(s) 16, 449, 673
HpaII CCGG 3 cut(s) 62, 163, 662
HphI GGTGA 2 cut(s) 252, 668
Hpy188I TCNGA 4 cut(s) 129, 184, 454, 525
Hpy188III TCNNGA 3 cut(s) 163, 562, 662
HpyAV CCTTC 4 cut(s) 36, 96, 128, 238
HpyCH4III ACNGT 3 cut(s) 99, 368, 733
HpyCH4IV ACGT 3 cut(s) 78, 342, 576
HpyCH4V TGCA 4 cut(s) 518, 632, 716, 728
HpyF10VI GCNNNNNNNGC 1 cut(s) 303
HpyF3I CTNAG 5 cut(s) 126, 204, 522, 621, 680
HpySE526I ACGT 3 cut(s) 78, 342, 576
Hsp92II CATG 4 cut(s) 301, 487, 557, 802
Kpn2I TCCGGA 2 cut(s) 162, 661
Kzo9I GATC 3 cut(s) 220, 558, 613
LguI GCTCTTC 1 cut(s) 296
Lsp1109I GCAGC 3 cut(s) 376, 872, 875
LweI GCATC 1 cut(s) 715
MaeI CTAG 2 cut(s) 42, 837
MaeII ACGT 3 cut(s) 78, 342, 576
MaeIII GTNAC 3 cut(s) 102, 345, 784
MalI GATC 3 cut(s) 222, 560, 615
MboI GATC 3 cut(s) 220, 558, 613
MboII GAAGA 9 cut(s) 250, 253, 313, 623, 707, 731, 806, 856, 859
MlsI TGGCCA 1 cut(s) 596
MluCI AATT 3 cut(s) 329, 473, 830
MluNI TGGCCA 1 cut(s) 596
MlyI GAGTC 3 cut(s) 10, 458, 667
Mox20I TGGCCA 1 cut(s) 596
MroI TCCGGA 2 cut(s) 162, 661
MscI TGGCCA 1 cut(s) 596
MseI TTAA 3 cut(s) 419, 548, 877
Msp20I TGGCCA 1 cut(s) 596
MspI CCGG 3 cut(s) 62, 163, 662
MspR9I CCNGG 2 cut(s) 446, 598
MvaI CCWGG 2 cut(s) 446, 598
MwoI GCNNNNNNNGC 1 cut(s) 303
NcoI CCATGG 1 cut(s) 483
NdeI CATATG 1 cut(s) 691
NdeII GATC 3 cut(s) 220, 558, 613
NlaIII CATG 4 cut(s) 301, 487, 557, 802
NlaIV GGNNCC 1 cut(s) 190
PciSI GCTCTTC 1 cut(s) 296
PcsI WCGNNNNNNNCGW 1 cut(s) 180
PfoI TCCNGGA 1 cut(s) 444
PkrI GCNGC 4 cut(s) 178, 391, 862, 865
PleI GAGTC 3 cut(s) 10, 457, 667
PpsI GAGTC 3 cut(s) 10, 457, 667
Psp6I CCWGG 2 cut(s) 444, 596
PspGI CCWGG 2 cut(s) 444, 596
PspN4I GGNNCC 1 cut(s) 190
RsaI GTAC 2 cut(s) 579, 685
RsaNI GTAC 2 cut(s) 578, 684
SapI GCTCTTC 1 cut(s) 296
SaqAI TTAA 3 cut(s) 419, 548, 877
SatI GCNGC 4 cut(s) 177, 390, 861, 864
Sau3AI GATC 3 cut(s) 220, 558, 613
ScaI AGTACT 1 cut(s) 685
SchI GAGTC 3 cut(s) 10, 458, 667
ScrFI CCNGG 2 cut(s) 446, 598
SfaNI GCATC 1 cut(s) 715
SmlI CTYRAG 1 cut(s) 815
SmoI CTYRAG 1 cut(s) 815
Sse9I AATT 3 cut(s) 329, 473, 830
SsiI CCGC 4 cut(s) 55, 157, 177, 198
SspMI CTAG 2 cut(s) 42, 837
StyD4I CCNGG 2 cut(s) 444, 596
StyI CCWWGG 1 cut(s) 483
TaaI ACNGT 3 cut(s) 99, 368, 733
TaiI ACGT 3 cut(s) 81, 345, 579
TaqI TCGA 2 cut(s) 609, 777
TasI AATT 3 cut(s) 329, 473, 830
TatI WGTACW 1 cut(s) 683
TauI GCSGC 1 cut(s) 179
Tru1I TTAA 3 cut(s) 419, 548, 877
Tru9I TTAA 3 cut(s) 419, 548, 877
TscAI CASTG 2 cut(s) 141, 373
TseI GCWGC 3 cut(s) 389, 860, 863
TspDTI ATGAA 4 cut(s) 314, 678, 708, 786
TspGWI ACGGA 2 cut(s) 125, 640
TspRI CASTG 2 cut(s) 141, 373
XapI RAATTY 1 cut(s) 329
XcmI CCANNNNNNNNNTGG 2 cut(s) 289, 796
XspI CTAG 2 cut(s) 42, 837
ZrmI AGTACT 1 cut(s) 685
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.