Rh2AG670400
NAC Family

inactive shikimate kinase like 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0013212
Physical Location & Seq
Forward (+)
88413927 .. 88416127
2201 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG670400.1

Sequence Viewer

Length: 879 bp
ATGGAGATAACAAGGACTCTCCTCCCTTCCTCCTCTTGGTCTAGTTTCCACCTCCGCACTCCGCTCCTCCATTTTCACGTCTCGCCTTCGCACCCACAGTCGTTACCCCCGGACGTCCTTCAGTCTCAGACCTCACTGCCCATTCCATCTCGCACCCGCTTCCGGAAACCTCTCGCCGCCTCCGATGGCACCACTTCCGCAACTAAGGTTGCTGTGGATGATCCCTCTCTTGCTGTGAAGAAGAAGGCAATGGATGTATCACCAGAACTCAAAGGAACTACCATATTCCTTGTGGGCATGAAGAGCTCCATAAAGACCAGTTTGGGGAAATTCCTCGCCAACGTGTTACGATACTATTACTTTGACAGTGATAGTTTGGTTGAGGAAGCTGCTGGCACTCAATCTGCCACCAAATCTTTAAGGGAGACTGACAAGAGTGGTTTCCAGGAGTCCGAGACTGAAGTGTTGAAGCAATTATCATCCATGGGTCGGTTAGTGGTCTGTGCTGGAGATGGTGCAACTCAGAGTTCAACAAATCTGGCTCTTTTAAGACATGGGATCACGATATGGATTGACGTACCTTTAGACATTGTGGCCAGGGGTGTGGTCGAAGATCAAACTCAGCTCTCTGCATACAATCTATCTACTTCCGTATCCTATCCAGAGGTTTTGACTCACCTAAGTACTTCATATGAAGAGTTGAGAGGTGGATATGCAATAGCTGATGCAACCGTATCTCTTCAGCAAGTAGCTGGTAAGTTAGGTTATGATGAAATCGACGATGTAACCCCCGAAGACATGGCTTTGGAGGTTCTCAAGGAGGTAGAGAAATTGACCAGAGTGAAGAAGATGATGGAAGCAGCAGCAAGACCTTTTTAA

Protein Analysis

292

Amino Acids

31.84

Weight (kDa)

5.74

Isoelectric Point (pI)

44.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SKI PF01202 103 - 225 1.9e-23 Shikimate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013212)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26900 AT3G26900 AT3G26900
fragaria_vesca FvH4_6g54030 FvH4_6g54030
malus_domestica MD09G1006100.v1.1 MD17G1010100.v1.1
prunus_persica Prupe.3G310900_v2.0.a1
pyrus_communis pycom17g00460
rosa_chinensis RchiOBHm_Chr2g0176211
rosa_laevigata RLG00000022390
rosa_multiflora Rmu_co8135912.1_g000001
rosa_rugosa Rorug02G0591800
rosa_samantha Rh2AG670400 Rh2BG681900 Rh2CG644700 Rh2DG695500
rosa_wichuraiana Rw2G054990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 117
AccB1I GGYRCC 1 cut(s) 188
AccBSI CCGCTC 1 cut(s) 64
AccIII TCCGGA 1 cut(s) 162
AciI CCGC 5 cut(s) 55, 62, 157, 177, 198
AclWI GGATC 2 cut(s) 215, 566
AcoI YGGCCR 1 cut(s) 594
AcsI RAATTY 1 cut(s) 329
AcuI CTGAAG 3 cut(s) 104, 480, 725
AcyI GRCGYC 1 cut(s) 114
AfaI GTAC 2 cut(s) 579, 685
AfiI CCNNNNNNNGG 4 cut(s) 36, 162, 324, 489
AflIII ACRYGT 1 cut(s) 342
AgsI TTSAA 2 cut(s) 469, 531
AjiI CACGTC 1 cut(s) 79
AjnI CCWGG 2 cut(s) 444, 596
AluBI AGCT 5 cut(s) 306, 389, 625, 722, 752
AluI AGCT 5 cut(s) 306, 389, 625, 722, 752
Alw21I GWGCWC 1 cut(s) 308
Alw26I GTCTC 4 cut(s) 85, 129, 419, 449
AlwI GGATC 2 cut(s) 215, 566
Aor13HI TCCGGA 1 cut(s) 162
AoxI GGCC 1 cut(s) 594
ApeKI GCWGC 3 cut(s) 389, 860, 863
ApoI RAATTY 1 cut(s) 329
AsuC2I CCSGG 1 cut(s) 110
AsuHPI GGTGA 2 cut(s) 252, 668
BaeI ACNNNNGTAYC 2 cut(s) 636, 669
BalI TGGCCA 1 cut(s) 596
BanI GGYRCC 1 cut(s) 188
BanII GRGCYC 1 cut(s) 308
BbsI GAAGAC 1 cut(s) 801
Bbv12I GWGCWC 1 cut(s) 308
BbvI GCAGC 3 cut(s) 376, 872, 875
BccI CCATC 4 cut(s) 154, 179, 506, 847
BciT130I CCWGG 2 cut(s) 446, 598
BciVI GTATCC 1 cut(s) 664
BcnI CCSGG 1 cut(s) 110
BcoDI GTCTC 4 cut(s) 85, 129, 419, 449
BfaI CTAG 1 cut(s) 42
BfuI GTATCC 1 cut(s) 664
BisI GCNGC 4 cut(s) 177, 390, 861, 864
BlsI GCNGC 4 cut(s) 178, 391, 862, 865
BmcAI AGTACT 1 cut(s) 685
Bme1390I CCNGG 3 cut(s) 110, 446, 598
BmgBI CACGTC 1 cut(s) 79
BmiI GGNNCC 1 cut(s) 190
BmrFI CCNGG 3 cut(s) 110, 446, 598
BmsI GCATC 1 cut(s) 715
BpiI GAAGAC 1 cut(s) 801
BpmI CTGGAG 1 cut(s) 528
BpuEI CTTGAG 1 cut(s) 800
BpuMI CCSGG 1 cut(s) 110
BsaHI GRCGYC 1 cut(s) 114
BsaJI CCNNGG 3 cut(s) 108, 483, 597
BsaWI WCCGGW 1 cut(s) 162
Bsc4I CCNNNNNNNGG 4 cut(s) 36, 162, 324, 489
Bse1I ACTGG 1 cut(s) 318
Bse3DI GCAATG 1 cut(s) 255
BseAI TCCGGA 1 cut(s) 162
BseBI CCWGG 2 cut(s) 446, 598
BseDI CCNNGG 3 cut(s) 108, 483, 597
BseGI GGATG 3 cut(s) 223, 259, 479
BseLI CCNNNNNNNGG 4 cut(s) 36, 162, 324, 489
BseMI GCAATG 1 cut(s) 255
BseMII CTCAG 3 cut(s) 140, 536, 635
BseNI ACTGG 1 cut(s) 318
BseRI GAGGAG 3 cut(s) 11, 22, 56
BseXI GCAGC 3 cut(s) 376, 872, 875
BshFI GGCC 1 cut(s) 596
BshNI GGYRCC 1 cut(s) 188
BsiHKAI GWGCWC 1 cut(s) 308
BsiSI CCGG 2 cut(s) 110, 163
BslI CCNNNNNNNGG 4 cut(s) 36, 162, 324, 489
BsmAI GTCTC 4 cut(s) 85, 129, 419, 449
BsmBI CGTCTC 1 cut(s) 85
BsnI GGCC 1 cut(s) 596
Bsp1286I GDGCHC 1 cut(s) 308
Bsp13I TCCGGA 1 cut(s) 162
Bsp143I GATC 3 cut(s) 220, 558, 613
Bsp19I CCATGG 1 cut(s) 483
BspACI CCGC 5 cut(s) 55, 62, 157, 177, 198
BspANI GGCC 1 cut(s) 596
BspCNI CTCAG 3 cut(s) 139, 535, 634
BspEI TCCGGA 1 cut(s) 162
BspLI GGNNCC 1 cut(s) 190
BspPI GGATC 2 cut(s) 215, 566
BspQI GCTCTTC 1 cut(s) 296
BspT107I GGYRCC 1 cut(s) 188
BsrBI CCGCTC 1 cut(s) 64
BsrDI GCAATG 1 cut(s) 255
BsrI ACTGG 1 cut(s) 318
BssECI CCNNGG 3 cut(s) 108, 483, 597
BssMI GATC 3 cut(s) 220, 558, 613
BssNI GRCGYC 1 cut(s) 114
BssT1I CCWWGG 1 cut(s) 483
Bst2UI CCWGG 2 cut(s) 446, 598
Bst4CI ACNGT 3 cut(s) 99, 368, 733
Bst6I CTCTTC 3 cut(s) 296, 690, 744
BstACI GRCGYC 1 cut(s) 114
BstC8I GCNNGC 1 cut(s) 394
BstDEI CTNAG 5 cut(s) 126, 204, 522, 621, 680
BstDSI CCRYGG 1 cut(s) 483
BstF5I GGATG 3 cut(s) 223, 259, 479
BstKTI GATC 3 cut(s) 223, 561, 616
BstMAI GTCTC 4 cut(s) 85, 129, 419, 449
BstMBI GATC 3 cut(s) 220, 558, 613
BstMWI GCNNNNNNNGC 1 cut(s) 303
BstNI CCWGG 2 cut(s) 446, 598
BstSCI CCNGG 3 cut(s) 108, 444, 596
BstV1I GCAGC 3 cut(s) 376, 872, 875
BstV2I GAAGAC 1 cut(s) 801
BstXI CCANNNNNNTGG 1 cut(s) 604
BsuI GTATCC 1 cut(s) 664
BsuRI GGCC 1 cut(s) 596
BtgI CCRYGG 1 cut(s) 483
BtrI CACGTC 1 cut(s) 79
BtsCI GGATG 3 cut(s) 223, 259, 479
BtsI GCAGTG 1 cut(s) 134
BtsIMutI CAGTG 2 cut(s) 134, 373
Cac8I GCNNGC 1 cut(s) 394
Csp6I GTAC 2 cut(s) 578, 684
CviAII CATG 4 cut(s) 298, 484, 554, 799
CviJI RGCY 8 cut(s) 306, 389, 542, 596, 625, 722, 752, 803
CviKI_1 RGCY 8 cut(s) 306, 389, 542, 596, 625, 722, 752, 803
CviQI GTAC 2 cut(s) 578, 684
DdeI CTNAG 5 cut(s) 126, 204, 522, 621, 680
DpnI GATC 3 cut(s) 222, 560, 615
DpnII GATC 3 cut(s) 220, 558, 613
EaeI YGGCCR 1 cut(s) 594
Eam1104I CTCTTC 3 cut(s) 296, 690, 744
EarI CTCTTC 3 cut(s) 296, 690, 744
Ecl136II GAGCTC 1 cut(s) 306
Eco130I CCWWGG 1 cut(s) 483
Eco24I GRGCYC 1 cut(s) 308
Eco53kI GAGCTC 1 cut(s) 306
Eco57I CTGAAG 3 cut(s) 104, 480, 725
EcoICRI GAGCTC 1 cut(s) 306
EcoRII CCWGG 2 cut(s) 444, 596
EcoT14I CCWWGG 1 cut(s) 483
EcoT38I GRGCYC 1 cut(s) 308
ErhI CCWWGG 1 cut(s) 483
Esp3I CGTCTC 1 cut(s) 85
FaeI CATG 4 cut(s) 301, 487, 557, 802
FatI CATG 4 cut(s) 297, 483, 553, 798
FauI CCCGC 1 cut(s) 164
FauNDI CATATG 1 cut(s) 691
Fnu4HI GCNGC 4 cut(s) 177, 390, 861, 864
FokI GGATG 3 cut(s) 230, 266, 466
FriOI GRGCYC 1 cut(s) 308
Fsp4HI GCNGC 4 cut(s) 177, 390, 861, 864
FspBI CTAG 1 cut(s) 42
GluI GCNGC 4 cut(s) 177, 390, 861, 864
GsuI CTGGAG 1 cut(s) 528
HaeIII GGCC 1 cut(s) 596
HapII CCGG 2 cut(s) 110, 163
Hin1I GRCGYC 1 cut(s) 114
Hin1II CATG 4 cut(s) 301, 487, 557, 802
HinfI GANTC 3 cut(s) 16, 449, 673
HpaII CCGG 2 cut(s) 110, 163
HphI GGTGA 2 cut(s) 252, 668
Hpy188I TCNGA 4 cut(s) 129, 184, 454, 525
Hpy188III TCNNGA 3 cut(s) 163, 562, 662
Hpy99I CGWCG 1 cut(s) 782
HpyAV CCTTC 4 cut(s) 36, 96, 128, 238
HpyCH4III ACNGT 3 cut(s) 99, 368, 733
HpyCH4IV ACGT 4 cut(s) 78, 114, 342, 576
HpyCH4V TGCA 4 cut(s) 518, 632, 716, 728
HpyF10VI GCNNNNNNNGC 1 cut(s) 303
HpyF3I CTNAG 5 cut(s) 126, 204, 522, 621, 680
HpySE526I ACGT 4 cut(s) 78, 114, 342, 576
Hsp92I GRCGYC 1 cut(s) 114
Hsp92II CATG 4 cut(s) 301, 487, 557, 802
Kpn2I TCCGGA 1 cut(s) 162
Kzo9I GATC 3 cut(s) 220, 558, 613
LguI GCTCTTC 1 cut(s) 296
LmnI GCTCC 2 cut(s) 69, 311
Lsp1109I GCAGC 3 cut(s) 376, 872, 875
LweI GCATC 1 cut(s) 715
MaeI CTAG 1 cut(s) 42
MaeII ACGT 4 cut(s) 78, 114, 342, 576
MaeIII GTNAC 3 cut(s) 102, 345, 784
MalI GATC 3 cut(s) 222, 560, 615
MbiI CCGCTC 1 cut(s) 64
MboI GATC 3 cut(s) 220, 558, 613
MboII GAAGA 9 cut(s) 250, 253, 313, 623, 707, 731, 806, 856, 859
MhlI GDGCHC 1 cut(s) 308
MlsI TGGCCA 1 cut(s) 596
MluCI AATT 3 cut(s) 329, 473, 830
MluNI TGGCCA 1 cut(s) 596
MlyI GAGTC 3 cut(s) 10, 458, 667
Mox20I TGGCCA 1 cut(s) 596
MroI TCCGGA 1 cut(s) 162
MscI TGGCCA 1 cut(s) 596
MseI TTAA 3 cut(s) 419, 548, 877
Msp20I TGGCCA 1 cut(s) 596
MspI CCGG 2 cut(s) 110, 163
MspR9I CCNGG 3 cut(s) 110, 446, 598
MvaI CCWGG 2 cut(s) 446, 598
MwoI GCNNNNNNNGC 1 cut(s) 303
NciI CCSGG 1 cut(s) 110
NcoI CCATGG 1 cut(s) 483
NdeI CATATG 1 cut(s) 691
NdeII GATC 3 cut(s) 220, 558, 613
NlaIII CATG 4 cut(s) 301, 487, 557, 802
NlaIV GGNNCC 1 cut(s) 190
PciSI GCTCTTC 1 cut(s) 296
PcsI WCGNNNNNNNCGW 1 cut(s) 180
PfoI TCCNGGA 1 cut(s) 444
PkrI GCNGC 4 cut(s) 178, 391, 862, 865
PleI GAGTC 3 cut(s) 10, 457, 667
PpsI GAGTC 3 cut(s) 10, 457, 667
Psp124BI GAGCTC 1 cut(s) 308
Psp6I CCWGG 2 cut(s) 444, 596
PspGI CCWGG 2 cut(s) 444, 596
PspN4I GGNNCC 1 cut(s) 190
RsaI GTAC 2 cut(s) 579, 685
RsaNI GTAC 2 cut(s) 578, 684
SacI GAGCTC 1 cut(s) 308
SapI GCTCTTC 1 cut(s) 296
SaqAI TTAA 3 cut(s) 419, 548, 877
SatI GCNGC 4 cut(s) 177, 390, 861, 864
Sau3AI GATC 3 cut(s) 220, 558, 613
ScaI AGTACT 1 cut(s) 685
SchI GAGTC 3 cut(s) 10, 458, 667
ScrFI CCNGG 3 cut(s) 110, 446, 598
SduI GDGCHC 1 cut(s) 308
SfaNI GCATC 1 cut(s) 715
SmlI CTYRAG 1 cut(s) 815
SmoI CTYRAG 1 cut(s) 815
Sse9I AATT 3 cut(s) 329, 473, 830
SsiI CCGC 5 cut(s) 55, 62, 157, 177, 198
SspMI CTAG 1 cut(s) 42
SstI GAGCTC 1 cut(s) 308
StyD4I CCNGG 3 cut(s) 108, 444, 596
StyI CCWWGG 1 cut(s) 483
TaaI ACNGT 3 cut(s) 99, 368, 733
TaiI ACGT 4 cut(s) 81, 117, 345, 579
TaqI TCGA 2 cut(s) 609, 777
TasI AATT 3 cut(s) 329, 473, 830
TatI WGTACW 1 cut(s) 683
TauI GCSGC 1 cut(s) 179
Tru1I TTAA 3 cut(s) 419, 548, 877
Tru9I TTAA 3 cut(s) 419, 548, 877
TscAI CASTG 2 cut(s) 141, 373
TseI GCWGC 3 cut(s) 389, 860, 863
TspDTI ATGAA 4 cut(s) 314, 678, 708, 786
TspGWI ACGGA 1 cut(s) 640
TspRI CASTG 2 cut(s) 141, 373
XapI RAATTY 1 cut(s) 329
XcmI CCANNNNNNNNNTGG 1 cut(s) 289
XspI CTAG 1 cut(s) 42
ZraI GACGTC 1 cut(s) 115
ZrmI AGTACT 1 cut(s) 685
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.