Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0010255

Physical Location & Seq

Genomic Coordinates Forward (+)

39566844 .. 39570589

Gene Structure 3746 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g54180.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1128 bp

ATGGCGAGCAGGTACTGGGTGGTGTCTCTGCCTGTTCAAAACTCGGCGTCGTCTTTATGGAACCGCCTCCAGGATCAAATCTCCAAGCATTCCTTCGACACTCCGCTCTACAGATTTCACATTCCCAATCTCCGCGTCGGAACCCTCGATTCTCTTCTCTCCCTCAGCGACGATCTCTTAAAGTCCAACAACTTCGTAGAGGGTGTTTCTCACAAGATCAGGCGTCAGATCGAGGAGCTTGAGAAGATTTCTGGAGTGGAGAGCAGCGCCCTCACTGTCGATGGAGTACCTGTGGATTCATATCTCACTAGATTCGTTTGGGATGAAGCGAAGTACCCGACAATGTCTCCTTTGAAGGAGATCATAGACAGCATTCATGGTCAAGTTGCAAAGATCGAGGATGATCTCAAGGTTCGGGTAGCTGAGTATAACAATGTCCGCAGTCAACTTAATGCGATCAACCGAAAGCAAAGCGGAAGCTTAGCAGTCCGTGATCTTTCCAATTTAGTCAAATCAGAGGACATTGTTGTCTCGGAACATCTGGTGACTCTCCTTGCAGTTGTTCTGAAGTACTCTCAGAAGGATTGGCTGTCAAGCTATGAGACCTTGACTAGTTATGTGGTCCCTAGGTCCTCTAAGAAGTTATTTGAAGATAATGAATATGCTCTTTACAGCGTAACACTGTTTGGTCGGGTTGCAGATAATTTTAGAACAAGCGCTCGTGAAAAGGGATTTCAAATTCGTGATTTCGAATATAGTTCTGAAGCACAAGAAAGTAGAAAGCAGGAGTTGGAAAAATTGGTACAAGACCAAGAAAATTTAAGAAGCTCGCTTCTGCAGTGGTGCTATACCAGTTACGGGGAGGTGTTCAGCTCCTGGATGCATTTTTGTGCTGTGCGTGTGTTTGCTGAGAGTATTCTGAGATATGGTTTACCACCATCTTTCTTGGCATGTGTTTTAGCTCCAGTTACAAAAAGTGAGAAGAAAGTACGATCGATCCTTGAAGGGCTGTGCGACAGTTCTAACAGTTTCTGGAAAACTGAAGATGAAGTGGGGGGAGCTATGGCTGGTCTAGCAGGTGATGCGGATGCGCATCCGTATGTTTCATTTAATATCAATCTTGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000221 GO:0000325 GO:0000902 GO:0003674 GO:0003824 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0008324 GO:0008553 GO:0009507 GO:0009536 GO:0009653 GO:0009826 GO:0009987 GO:0012505 GO:0015075 GO:0015077 GO:0015078 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0015672 GO:0016020 GO:0016043 GO:0016049 GO:0016462 GO:0016469 GO:0016471 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0031090 GO:0032502 GO:0032989 GO:0032991 GO:0033176 GO:0033178 GO:0033180 GO:0034220 GO:0036442 GO:0040007 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0048589 GO:0048856 GO:0048869 GO:0051179 GO:0051234 GO:0055085 GO:0060560 GO:0071840 GO:0071944 GO:0090662 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098796 GO:0098805 GO:0099131 GO:0099132 GO:1902600

KEGG Pathways

Metabolic & Signaling

ko00190 ko01100 ko04145 ko04150 ko04721 ko04966 ko05110 ko05120 ko05323 map00190 map01100 map04145 map04150 map04721 map04966 map05110 map05120 map05323

Pfam Domains

Protein Families

V-ATPase_C

Protein Analysis

376

Amino Acids

42.48

Weight (kDa)

5.63

Isoelectric Point (pI)

50.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
V-ATPase_C	PF03223	5 - 339	8.1e-126	V-ATPase subunit C

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010255)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G12840
fragaria_vesca	FvH4_6g54180 FvH4_6g54180 FvH4_6g54180
malus_domestica	MD09G1004500.v1.1 MD17G1008500.v1.1
prunus_persica	Prupe.3G312200_v2.0.a1 Prupe.3G312200_v2.0.a1
pyrus_communis	pycom111g00330 pycom17g00350
rosa_chinensis	RchiOBHm_Chr2g0176361
rosa_laevigata	RLG00000022406
rosa_multiflora	Rmu_sc0009140.1_g000001 Rmu_sc0027060.1_g000004 Rmu_sc0035399.1_g000001
rosa_roxburghii	Rroxscaffold_2G00076540
rosa_rugosa	Rorug02G0593500
rosa_samantha	Rh2AG672000 Rh2BG683500 Rh2DG697000
rosa_wichuraiana	Rw2G055110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1067
AasI	GACNNNNNNGTC	1 cut(s)	527
Acc16I	TGCGCA	1 cut(s)	1092
Acc36I	ACCTGC	1 cut(s)	1067
AccBSI	CCGCTC	1 cut(s)	106
AccII	CGCG	1 cut(s)	135
AciI	CCGC	6 cut(s)	64, 104, 133, 439, 474, 1085
AclWI	GGATC	2 cut(s)	81, 991
AcsI	RAATTY	2 cut(s)	738, 817
AcuI	CTGAAG	3 cut(s)	587, 783, 1062
AcyI	GRCGYC	2 cut(s)	47, 223
AfaI	GTAC	6 cut(s)	14, 288, 335, 572, 804, 990
AfeI	AGCGCT	1 cut(s)	718
AfiI	CCNNNNNNNGG	2 cut(s)	70, 858
AgsI	TTSAA	5 cut(s)	38, 355, 650, 737, 1004
AhlI	ACTAGT	1 cut(s)	611
AjnI	CCWGG	2 cut(s)	69, 875
AjuI	GAANNNNNNNTTGG	2 cut(s)	77, 109
AluBI	AGCT	8 cut(s)	238, 422, 480, 597, 828, 873, 962, 1061
AluI	AGCT	8 cut(s)	238, 422, 480, 597, 828, 873, 962, 1061
Alw26I	GTCTC	4 cut(s)	30, 351, 535, 596
AlwI	GGATC	2 cut(s)	81, 991
AlwNI	CAGNNNCTG	3 cut(s)	15, 876, 1032
Aor51HI	AGCGCT	1 cut(s)	718
ApeKI	GCWGC	1 cut(s)	264
ApoI	RAATTY	2 cut(s)	738, 817
AspA2I	CCTAGG	1 cut(s)	626
AspLEI	GCGC	3 cut(s)	269, 719, 1093
AspS9I	GGNCC	2 cut(s)	622, 630
AsuHPI	GGTGA	2 cut(s)	556, 1091
AsuII	TTCGAA	1 cut(s)	750
AvaII	GGWCC	2 cut(s)	622, 630
AvrII	CCTAGG	1 cut(s)	626
BauI	CACGAG	1 cut(s)	720
BbvCI	CCTCAGC	1 cut(s)	164
BbvI	GCAGC	1 cut(s)	276
BccI	CCATC	2 cut(s)	275, 946
BciT130I	CCWGG	2 cut(s)	71, 877
BcoDI	GTCTC	4 cut(s)	30, 351, 535, 596
BcuI	ACTAGT	1 cut(s)	611
BfaI	CTAG	4 cut(s)	309, 612, 627, 1073
BfmI	CTRYAG	2 cut(s)	109, 836
BfoI	RGCGCY	2 cut(s)	270, 720
BfuAI	ACCTGC	1 cut(s)	1067
BisI	GCNGC	1 cut(s)	265
BlnI	CCTAGG	1 cut(s)	626
BlpI	GCTNAGC	1 cut(s)	481
BlsI	GCNGC	1 cut(s)	266
BmcAI	AGTACT	1 cut(s)	572
Bme1390I	CCNGG	2 cut(s)	71, 877
Bme18I	GGWCC	2 cut(s)	622, 630
BmgT120I	GGNCC	2 cut(s)	622, 630
BmiI	GGNNCC	3 cut(s)	62, 142, 624
BmrFI	CCNGG	2 cut(s)	71, 877
BmrI	ACTGGG	1 cut(s)	25
BmsI	GCATC	4 cut(s)	870, 1072, 1078, 1102
BmuI	ACTGGG	1 cut(s)	25
BpmI	CTGGAG	3 cut(s)	53, 273, 948
Bpu10I	CCTNAGC	1 cut(s)	164
Bpu1102I	GCTNAGC	1 cut(s)	481
Bpu14I	TTCGAA	1 cut(s)	750
BpuEI	CTTGAG	2 cut(s)	260, 392
Bsa29I	ATCGAT	1 cut(s)	995
BsaBI	GATNNNNATC	2 cut(s)	300, 1092
BsaHI	GRCGYC	2 cut(s)	47, 223
BsaI	GGTCTC	1 cut(s)	596
BsaJI	CCNNGG	1 cut(s)	626
BsaXI	ACNNNNNCTCC	4 cut(s)	276, 306, 331, 361
Bsc4I	CCNNNNNNNGG	2 cut(s)	70, 858
Bse1I	ACTGG	3 cut(s)	20, 852, 965
Bse8I	GATNNNNATC	2 cut(s)	300, 1092
BseBI	CCWGG	2 cut(s)	71, 877
BseCI	ATCGAT	1 cut(s)	995
BseDI	CCNNGG	1 cut(s)	626
BseGI	GGATG	5 cut(s)	328, 406, 885, 1093, 1093
BseJI	GATNNNNATC	2 cut(s)	300, 1092
BseLI	CCNNNNNNNGG	2 cut(s)	70, 858
BseMII	CTCAG	5 cut(s)	178, 414, 590, 900, 911
BseNI	ACTGG	3 cut(s)	20, 852, 965
BseRI	GAGGAG	1 cut(s)	248
BseXI	GCAGC	1 cut(s)	276
Bsh1236I	CGCG	1 cut(s)	135
Bsh1285I	CGRYCG	1 cut(s)	995
BshVI	ATCGAT	1 cut(s)	995
BsiEI	CGRYCG	1 cut(s)	995
BslFI	GGGAC	1 cut(s)	608
BslI	CCNNNNNNNGG	2 cut(s)	70, 858
BsmAI	GTCTC	4 cut(s)	30, 351, 535, 596
BsmFI	GGGAC	1 cut(s)	608
BsmI	GAATGC	2 cut(s)	88, 372
Bso31I	GGTCTC	1 cut(s)	596
Bsp119I	TTCGAA	1 cut(s)	750
Bsp1720I	GCTNAGC	1 cut(s)	481
BspACI	CCGC	6 cut(s)	64, 104, 133, 439, 474, 1085
BspCNI	CTCAG	5 cut(s)	177, 415, 589, 901, 912
BspDI	ATCGAT	1 cut(s)	995
BspFNI	CGCG	1 cut(s)	135
BspLI	GGNNCC	3 cut(s)	62, 142, 624
BspMAI	CTGCAG	1 cut(s)	840
BspMI	ACCTGC	1 cut(s)	1067
BspPI	GGATC	2 cut(s)	81, 991
BspT104I	TTCGAA	1 cut(s)	750
BspTNI	GGTCTC	1 cut(s)	596
BsrBI	CCGCTC	1 cut(s)	106
BsrI	ACTGG	3 cut(s)	20, 852, 965
BssECI	CCNNGG	1 cut(s)	626
BssNI	GRCGYC	2 cut(s)	47, 223
BssSI	CACGAG	1 cut(s)	720
BssT1I	CCWWGG	1 cut(s)	626
Bst2BI	CACGAG	1 cut(s)	720
Bst2UI	CCWGG	2 cut(s)	71, 877
Bst4CI	ACNGT	4 cut(s)	277, 684, 1019, 1028
Bst6I	CTCTTC	1 cut(s)	159
BstACI	GRCGYC	2 cut(s)	47, 223
BstAPI	GCANNNNNTGC	1 cut(s)	1082
BstBI	TTCGAA	1 cut(s)	750
BstC8I	GCNNGC	2 cut(s)	7, 830
BstDEI	CTNAG	7 cut(s)	164, 423, 481, 576, 636, 909, 920
BstF5I	GGATG	5 cut(s)	328, 406, 885, 1093, 1093
BstFNI	CGCG	1 cut(s)	135
BstH2I	RGCGCY	2 cut(s)	270, 720
BstHHI	GCGC	3 cut(s)	269, 719, 1093
BstMAI	GTCTC	4 cut(s)	30, 351, 535, 596
BstMCI	CGRYCG	1 cut(s)	995
BstMWI	GCNNNNNNNGC	2 cut(s)	1073, 1082
BstNI	CCWGG	2 cut(s)	71, 877
BstNSI	RCATGY	1 cut(s)	954
BstSCI	CCNGG	2 cut(s)	69, 875
BstSFI	CTRYAG	2 cut(s)	109, 836
BstUI	CGCG	1 cut(s)	135
BstV1I	GCAGC	1 cut(s)	276
Bsu15I	ATCGAT	1 cut(s)	995
BsuTUI	ATCGAT	1 cut(s)	995
BtsCI	GGATG	5 cut(s)	328, 406, 885, 1093, 1093
BtsI	GCAGTG	1 cut(s)	845
BtsIMutI	CAGTG	3 cut(s)	273, 680, 845
BveI	ACCTGC	1 cut(s)	1067
Cac8I	GCNNGC	2 cut(s)	7, 830
CaiI	CAGNNNCTG	3 cut(s)	15, 876, 1032
CfoI	GCGC	3 cut(s)	269, 719, 1093
Cfr13I	GGNCC	2 cut(s)	622, 630
ClaI	ATCGAT	1 cut(s)	995
CseI	GACGC	3 cut(s)	36, 124, 212
Csp6I	GTAC	6 cut(s)	13, 287, 334, 571, 803, 989
CviAII	CATG	2 cut(s)	377, 951
CviQI	GTAC	6 cut(s)	13, 287, 334, 571, 803, 989
DdeI	CTNAG	7 cut(s)	164, 423, 481, 576, 636, 909, 920
DrdI	GACNNNNNNGTC	1 cut(s)	527
DseDI	GACNNNNNNGTC	1 cut(s)	527
Eam1104I	CTCTTC	1 cut(s)	159
EarI	CTCTTC	1 cut(s)	159
Eco130I	CCWWGG	1 cut(s)	626
Eco31I	GGTCTC	1 cut(s)	596
Eco47I	GGWCC	2 cut(s)	622, 630
Eco47III	AGCGCT	1 cut(s)	718
Eco57I	CTGAAG	3 cut(s)	587, 783, 1062
EcoO109I	RGGNCCY	1 cut(s)	630
EcoRII	CCWGG	2 cut(s)	69, 875
EcoT14I	CCWWGG	1 cut(s)	626
EcoT22I	ATGCAT	1 cut(s)	885
ErhI	CCWWGG	1 cut(s)	626
FaeI	CATG	2 cut(s)	380, 954
FalI	AAGNNNNNCTT	2 cut(s)	77, 109
FaqI	GGGAC	1 cut(s)	608
FatI	CATG	2 cut(s)	376, 950
Fnu4HI	GCNGC	1 cut(s)	265
FokI	GGATG	5 cut(s)	335, 413, 892, 1080, 1100
Fsp4HI	GCNGC	1 cut(s)	265
FspAI	RTGCGCAY	1 cut(s)	1092
FspBI	CTAG	4 cut(s)	309, 612, 627, 1073
FspI	TGCGCA	1 cut(s)	1092
GlaI	GCGC	3 cut(s)	268, 718, 1092
GluI	GCNGC	1 cut(s)	265
GsuI	CTGGAG	3 cut(s)	53, 273, 948
HaeII	RGCGCY	2 cut(s)	270, 720
HgaI	GACGC	3 cut(s)	36, 124, 212
HhaI	GCGC	3 cut(s)	269, 719, 1093
Hin1I	GRCGYC	2 cut(s)	47, 223
Hin1II	CATG	2 cut(s)	380, 954
Hin6I	GCGC	3 cut(s)	267, 717, 1091
HinP1I	GCGC	3 cut(s)	267, 717, 1091
HincII	GTYRAC	1 cut(s)	446
HindII	GTYRAC	1 cut(s)	446
HindIII	AAGCTT	1 cut(s)	478
HinfI	GANTC	4 cut(s)	149, 296, 312, 547
HphI	GGTGA	2 cut(s)	556, 1091
Hpy166II	GTNNAC	2 cut(s)	446, 932
Hpy188I	TCNGA	8 cut(s)	140, 228, 517, 535, 567, 579, 763, 921
Hpy188III	TCNNGA	4 cut(s)	252, 722, 743, 1033
Hpy8I	GTNNAC	2 cut(s)	446, 932
Hpy99I	CGWCG	3 cut(s)	52, 140, 173
HpyAV	CCTTC	4 cut(s)	103, 349, 574, 998
HpyCH4III	ACNGT	4 cut(s)	277, 684, 1019, 1028
HpyCH4V	TGCA	5 cut(s)	389, 557, 698, 838, 883
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1073, 1082
HpyF3I	CTNAG	7 cut(s)	164, 423, 481, 576, 636, 909, 920
Hsp92I	GRCGYC	2 cut(s)	47, 223
Hsp92II	CATG	2 cut(s)	380, 954
HspAI	GCGC	3 cut(s)	267, 717, 1091
LmnI	GCTCC	4 cut(s)	235, 878, 967, 1058
Lsp1109I	GCAGC	1 cut(s)	276
LweI	GCATC	4 cut(s)	870, 1072, 1078, 1102
MaeI	CTAG	4 cut(s)	309, 612, 627, 1073
MaeIII	GTNAC	4 cut(s)	544, 676, 854, 967
MbiI	CCGCTC	1 cut(s)	106
MboII	GAAGA	5 cut(s)	146, 256, 662, 994, 1055
MluCI	AATT	5 cut(s)	502, 703, 738, 797, 817
MlyI	GAGTC	1 cut(s)	541
MmeI	TCCRAC	3 cut(s)	118, 210, 771
Mph1103I	ATGCAT	1 cut(s)	885
MseI	TTAA	4 cut(s)	179, 450, 821, 1110
MslI	CAYNNNNRTG	2 cut(s)	888, 1098
MspR9I	CCNGG	2 cut(s)	71, 877
Mva1269I	GAATGC	2 cut(s)	88, 372
MvaI	CCWGG	2 cut(s)	71, 877
MvnI	CGCG	1 cut(s)	135
MwoI	GCNNNNNNNGC	2 cut(s)	1073, 1082
NlaIII	CATG	2 cut(s)	380, 954
NlaIV	GGNNCC	3 cut(s)	62, 142, 624
NmeAIII	GCCGAG	1 cut(s)	23
NmuCI	GTSAC	1 cut(s)	544
NsbI	TGCGCA	1 cut(s)	1092
NsiI	ATGCAT	1 cut(s)	885
NspI	RCATGY	1 cut(s)	954
NspV	TTCGAA	1 cut(s)	750
PaqCI	CACCTGC	1 cut(s)	1067
PcsI	WCGNNNNNNNCGW	1 cut(s)	144
PctI	GAATGC	2 cut(s)	88, 372
PfeI	GAWTC	3 cut(s)	149, 296, 312
PflFI	GACNNNGTC	1 cut(s)	343
PfoI	TCCNGGA	2 cut(s)	69, 875
PkrI	GCNGC	1 cut(s)	266
Ple19I	CGATCG	1 cut(s)	995
PleI	GAGTC	1 cut(s)	541
PpsI	GAGTC	1 cut(s)	541
PpuMI	RGGWCCY	1 cut(s)	630
Psp5II	RGGWCCY	1 cut(s)	630
Psp6I	CCWGG	2 cut(s)	69, 875
PspGI	CCWGG	2 cut(s)	69, 875
PspN4I	GGNNCC	3 cut(s)	62, 142, 624
PspPI	GGNCC	2 cut(s)	622, 630
PspPPI	RGGWCCY	1 cut(s)	630
PstI	CTGCAG	1 cut(s)	840
PstNI	CAGNNNCTG	3 cut(s)	15, 876, 1032
PsyI	GACNNNGTC	1 cut(s)	343
PvuI	CGATCG	1 cut(s)	995
RsaI	GTAC	6 cut(s)	14, 288, 335, 572, 804, 990
RsaNI	GTAC	6 cut(s)	13, 287, 334, 571, 803, 989
RseI	CAYNNNNRTG	2 cut(s)	888, 1098
SaqAI	TTAA	4 cut(s)	179, 450, 821, 1110
SatI	GCNGC	1 cut(s)	265
Sau96I	GGNCC	2 cut(s)	622, 630
ScaI	AGTACT	1 cut(s)	572
SchI	GAGTC	1 cut(s)	541
ScrFI	CCNGG	2 cut(s)	71, 877
SfaNI	GCATC	4 cut(s)	870, 1072, 1078, 1102
SfcI	CTRYAG	2 cut(s)	109, 836
SfuI	TTCGAA	1 cut(s)	750
SinI	GGWCC	2 cut(s)	622, 630
SmiMI	CAYNNNNRTG	2 cut(s)	888, 1098
SmlI	CTYRAG	2 cut(s)	239, 407
SmoI	CTYRAG	2 cut(s)	239, 407
SpeI	ACTAGT	1 cut(s)	611
Sse9I	AATT	5 cut(s)	502, 703, 738, 797, 817
SsiI	CCGC	6 cut(s)	64, 104, 133, 439, 474, 1085
SspMI	CTAG	4 cut(s)	309, 612, 627, 1073
StyD4I	CCNGG	2 cut(s)	69, 875
StyI	CCWWGG	1 cut(s)	626
TaaI	ACNGT	4 cut(s)	277, 684, 1019, 1028
TaqI	TCGA	7 cut(s)	96, 147, 231, 279, 396, 750, 995
TasI	AATT	5 cut(s)	502, 703, 738, 797, 817
TatI	WGTACW	1 cut(s)	570
TfiI	GAWTC	3 cut(s)	149, 296, 312
Tru1I	TTAA	4 cut(s)	179, 450, 821, 1110
Tru9I	TTAA	4 cut(s)	179, 450, 821, 1110
TscAI	CASTG	3 cut(s)	280, 687, 845
TseFI	GTSAC	1 cut(s)	544
TseI	GCWGC	1 cut(s)	264
Tsp45I	GTSAC	1 cut(s)	544
TspDTI	ATGAA	6 cut(s)	288, 339, 365, 672, 1062, 1095
TspGWI	ACGGA	2 cut(s)	479, 1086
TspRI	CASTG	3 cut(s)	280, 687, 845
Tth111I	GACNNNGTC	1 cut(s)	343
VpaK11BI	GGWCC	2 cut(s)	622, 630
XapI	RAATTY	2 cut(s)	738, 817
XceI	RCATGY	1 cut(s)	954
XmaJI	CCTAGG	1 cut(s)	626
XspI	CTAG	4 cut(s)	309, 612, 627, 1073
ZrmI	AGTACT	1 cut(s)	572
Zsp2I	ATGCAT	1 cut(s)	885

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.