FvH4_7g03720

Retinoblastoma-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0003674
Physical Location & Seq
Reverse (-)
4232572 .. 4240155
7584 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g03720.t2

Sequence Viewer

Length: 3081 bp
ATGAGTCCACCTGCTGTGCTGAAGATGGAAGATTCAAAGACGGAAGCTTCTGCGTCCACCAATTCGAATTCGGGGACTGGTGATTTGGACCCTGTCAAAGCTCGATTTACTGATTTCTGCAAGAATGGATTGTCCTTGGATGAGAACACGCTGACACAAGCTTTGAAGCTGCTATTAGAAACAAAACATGTTCTGATATCAAATTCATCAGCTATCGGAAATGGAACGCCTGAAGAAGCTGAGCGGTTCTGGTTTTCGTTTGTTTTGTTCTCGGTGAAAACATTGAATGAGAAGAGTTCCGATAATTCACAGAAGGCGTCTGATTATAATGGATTCTCTCTCATCCAGATTTTGAGAGCTGCTAAGCTCAACGTTGTGGATTTCTTTAAGGAGCTTCCACAGTTTATTGTTAAGGCCGGCCCAATTTTAAGTAATCTGTATGGCATAGATTGGGAGAGCAAACTTGAGGCAAAAGAGTTGCAGGCAAATTTTCTGTACTTGAGCCTTTTAAGCAGGTACTTCAAAAAATCATATGGGGAATTCTTCGCCAGTGATGCAACTGTTGATAAGAAGTCAGCCATTACCAATGGGACTGGTTATGTGTCAGAGTATCATAGATTTGGATGGCTGCTGTTTTTGGCCCTTCGGGTACACGTATTTGGGCGAGTTAGAGACCTGGTGACATGCACAAACGGCTTGGTTTCCATATTGGCAATTTTAATTATTCATGTCCCTGTTCGCTTCAGACATGCTAGAATCCTCGATTCAAAACGTTTTGTGAGAAAAGGAAACAAAGGTGTGGACCTTCTTGCATCACTTTGCAACATTTATGACACCTCAGAAGAAGAGTTGAGAAAAACAATGGAAAAGGCTAATACATTGATATCAGATCTCTTGAAGAAGAAGCCTTGTTTGGCATCTGAATGTAAAAATGAAAATCTAAAGAATATTGACACAGATGGTCTGACCTATTTTGAAGGACTTCTGGATGAACCATCTTTGTCATCTAGTTTAGATATTCTAGAAAAAGATCATGATGATGCGATTCGGAATAAGGGTGAACTGGATGAGAGAGTATTTATAAACGAGGAGGATACCTTATTTGGTTCAGGGAGCTTGTCTGGAGGGTCCTTGAATTTATCTGGGGTGAAGAGGAAAGTTGACTCAATGGCCTCCCCAACAAAGACAATTACAAGTCCACTTTCTCCCCACCGCTCTCCTGTCTCACAGGCTAATGGTGGGGCTAATTCAAAGATGGCAGCTACACCTGTGAGCACTGCAATGACAACTGCAAAGTGGCTTAGGACCTTTATTTCTCCACTTCCATCAAAACCCTCAGCCGAGCTCGAGGGTTTTCTGGCATCATGTGATAGAAACGTTAGTAACGATGTGATACGCAGGGCGGAAATAATACTTGAAGCCATATTTCCAAGTAGTTCTTTGGGGGATAGATGTGTAACTGGTAGTCTTCAAAGTGCTAACCTCATGGACAATGTGTGGGCAGAACAACGAAGACAAGAAGCACTCAAGCTTTATTATAGAGTTTTGGAAGCAATGTGTAGGGCAGAGGCCCAAGTTCTGCATGCTACCAATTTGACTTCTTTATTAACAAATGAGAGATTCCATAGATGCATGCTGGCATGCTCTGCTGAGTTAGTCCTGGCAACACATAAGACGGTCACAATGTTGTTTCCTGCAGTACTAGAAAGGACAGGTATTACAGCGTTTGATCTTAGCAAGGTGATAGAAAGCTTCATTAGACATGAAGAATCACTACCAAGAGAATTGAGACGGCATCTGAATTCATTGGAAGAACGACTTTTGGAGAGCATGGTATGGGAGAAGGGTTCCTCAATGTACAATTCTTTGACAGTTGCTAGGCCTGCCTTCTCGGCTGAAATTAACCGTCTCGGGTTATTAGCAGAACCAATGCCGTCTTTGGATGCGATAGCTTTGCAGATCAACTTCTCTTGTGGAGGGTTGCCTCCAGTTCCTTCTTTACAGAAGCATGAGACTTCCCCAGGTCAAAATGGAGATCTCAGGTCTCCAAAGAGACCCTGCACAGAACATAGAGGTGTATTACTGGAGCGAAATTCCTTTACTTCTCCTGTGAAAGATCGTCTCCTGGGCATAAGTAACCTTAAATCAAAGCTGCCACCGCCACCTTTACAATCTGCATTTGCAAGTCCAACACGGCCAAATCCAGGAGGCGGAGGGGAAACATGTGCAGAAACTGGAGTTAGTATTTTCTTTAATAAGATTATCAAGCTGGCTGCTGTGAGAATTAATGGTATGGTCGAAAGGTTGCAATTGTCTCAGCAAGTCAGGGAAAACGTTTATCGCCTCTTTCAACAAATCCTTGTTCAGCGCACATCCCTCTTCTTTAACCGCCATATTGACCAGATTATCTTGTGTTGTTTTTATGGGGTGGCCAAGATTTCTCAACTAAGTCTGACATTTAGGGAAATCATATACAACTACAGGAAGCAACCACAATGCAAACCACAAGTTTTCCGAAGTGTCTTTGTTGACTGGTCATCAGCTAGACGCAATGGGCGGCCTGGACAGGAACATGTAGATATTATAAGATTTTACAATGAAGTATTTATTCCTTCTGTCAAGACCCTGCTTGTGGAGCTCGGGCCTGCTACTACAACTACTAGAGCCAATGTAGCTCCTGAAGCTAACAATAACAATGACGCTCAATGTCCAGGATCACCTAAAGTATCCACCTTTCCAAGTCTCCCAGATATGTCTCCAAAGAAAGTATCTGCAGCACACAATGTTTATGTCTCTCCATTGCGATCATCAAAGATGGATGCCCTAATCTCACATAGCTCAAAGAGCTATTATGCTTGTGTTGGGGAAAGTACTCATGCGTATCAGAGCCCTTCGAAAGATCTCACAGCGATCAACAACCGCTTAAATGGCACACGAAAACGCACACTCGACTTTGACAACGTGGACGTAGGGGTTGGTTTGGTTAGTGATTCTATGGTGGCCAACAGCTTGTACCTTCAAAATGGGAGCTGTGCATCCTCTTCAGGTGGTCCATCCAAACCCGAGCAACCTGACTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000082 GO:0000278 GO:0000280 GO:0000902 GO:0000904 GO:0001558 GO:0001708 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006275 GO:0006349 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007059 GO:0007127 GO:0007129 GO:0007275 GO:0008134 GO:0008150 GO:0008152 GO:0008356 GO:0009553 GO:0009555 GO:0009566 GO:0009567 GO:0009653 GO:0009791 GO:0009888 GO:0009889 GO:0009987 GO:0010016 GO:0010026 GO:0010052 GO:0010090 GO:0010103 GO:0010374 GO:0010377 GO:0010468 GO:0010556 GO:0010564 GO:0016043 GO:0019219 GO:0019222 GO:0019953 GO:0022402 GO:0022414 GO:0022619 GO:0030154 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032875 GO:0032989 GO:0033043 GO:0034641 GO:0040008 GO:0040029 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0044770 GO:0044772 GO:0044843 GO:0045132 GO:0045143 GO:0045165 GO:0046483 GO:0048229 GO:0048285 GO:0048366 GO:0048367 GO:0048468 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051052 GO:0051128 GO:0051171 GO:0051239 GO:0051276 GO:0051301 GO:0051321 GO:0051704 GO:0051726 GO:0051783 GO:0055046 GO:0060255 GO:0061982 GO:0065007 GO:0070192 GO:0071514 GO:0071704 GO:0071840 GO:0080090 GO:0090329 GO:0090558 GO:0090626 GO:0090627 GO:0090698 GO:0097159 GO:0098813 GO:0099402 GO:0140013 GO:1901360 GO:1901363 GO:1903046 GO:1903047 GO:1903866 GO:2000036 GO:2000112 GO:2000653
KEGG Pathways
Metabolic & Signaling
ko04110 ko04218 ko04350 ko05165 ko05203 map04110 map04218 map04350 map05165 map05203
Pfam Domains
Protein Families
DUF3452 RB_A RB_B Rb_C

Protein Analysis

1027

Amino Acids

113.44

Weight (kDa)

8.74

Isoelectric Point (pI)

49.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3452 PF11934 106 - 243 3.9e-31 Domain of unknown function (DUF3452)
RB_A PF01858 422 - 623 4.2e-65 Retinoblastoma-associated protein A domain
RB_B PF01857 747 - 874 2.4e-43 Retinoblastoma-associated protein B domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 327, 1082, 2585
AarI CACCTGC 1 cut(s) 19
Acc36I ACCTGC 2 cut(s) 19, 504
AccBSI CCGCTC 2 cut(s) 244, 1215
AciI CCGC 8 cut(s) 244, 1213, 1403, 2159, 2211, 2389, 2557, 2920
AclI AACGTT 4 cut(s) 372, 772, 1377, 2334
AclWI GGATC 1 cut(s) 2722
AcoI YGGCCR 3 cut(s) 2195, 2430, 3000
AcsI RAATTY 7 cut(s) 67, 202, 487, 539, 1135, 1801, 2092
AcuI CTGAAG 5 cut(s) 41, 252, 727, 2700, 3027
AcyI GRCGYC 1 cut(s) 317
AfaI GTAC 7 cut(s) 497, 518, 651, 1701, 1859, 2872, 3014
AfiI CCNNNNNNNGG 3 cut(s) 2204, 2210, 2632
AflIII ACRYGT 4 cut(s) 187, 652, 2222, 2572
AjnI CCWGG 7 cut(s) 675, 1659, 2020, 2124, 2203, 2560, 2710
AjuI GAANNNNNNNTTGG 2 cut(s) 896, 928
Alw21I GWGCWC 3 cut(s) 1277, 1347, 2640
AlwI GGATC 1 cut(s) 2722
AlwNI CAGNNNCTG 1 cut(s) 2234
Ama87I CYCGRG 4 cut(s) 1346, 1910, 2639, 3062
ApeKI GCWGC 7 cut(s) 169, 359, 628, 1259, 2152, 2273, 2774
ApoI RAATTY 7 cut(s) 67, 202, 487, 539, 1135, 1801, 2092
ArsI GACNNNNNNTTYG 4 cut(s) 67, 99, 145, 177
AseI ATTAAT 1 cut(s) 2286
Asp700I GAANNNNTTC 1 cut(s) 981
AspLEI GCGC 1 cut(s) 2370
AspS9I GGNCC 9 cut(s) 88, 419, 640, 802, 1128, 1305, 1570, 2642, 3050
AsuHPI GGTGA 7 cut(s) 92, 286, 691, 1070, 1159, 1753, 2709
AsuII TTCGAA 2 cut(s) 65, 2894
AvaI CYCGRG 4 cut(s) 1346, 1910, 2639, 3062
AvaII GGWCC 5 cut(s) 88, 802, 1128, 1305, 3050
BalI TGGCCA 2 cut(s) 2432, 3002
BanII GRGCYC 3 cut(s) 1347, 2640, 2891
BarI GAAGNNNNNNTAC 2 cut(s) 1758, 1790
BbsI GAAGAC 2 cut(s) 1460, 1519
Bbv12I GWGCWC 3 cut(s) 1277, 1347, 2640
BbvCI CCTCAGC 1 cut(s) 1336
BbvI GCAGC 7 cut(s) 156, 346, 615, 1271, 2139, 2260, 2786
BccI CCATC 8 cut(s) 19, 618, 953, 1003, 1249, 1333, 2809, 3061
BceAI ACGGC 4 cut(s) 709, 1808, 1918, 2210
BcgI CGANNNNNNTGC 2 cut(s) 1936, 1970
BciT130I CCWGG 7 cut(s) 677, 1661, 2022, 2126, 2205, 2562, 2712
BciVI GTATCC 2 cut(s) 1087, 2737
BfaI CTAG 7 cut(s) 753, 1008, 1022, 1703, 1878, 2544, 2661
BfmI CTRYAG 3 cut(s) 1695, 2479, 2772
BfuAI ACCTGC 2 cut(s) 19, 504
BfuI GTATCC 2 cut(s) 1087, 2737
BglI GCCNNNNNGGC 1 cut(s) 1892
BglII AGATCT 3 cut(s) 889, 2035, 2899
BisI GCNGC 8 cut(s) 170, 360, 629, 1260, 2153, 2274, 2558, 2775
BlpI GCTNAGC 2 cut(s) 240, 363
BlsI GCNGC 8 cut(s) 171, 361, 630, 1261, 2154, 2275, 2559, 2776
BmcAI AGTACT 2 cut(s) 1701, 2872
Bme1390I CCNGG 7 cut(s) 677, 1661, 2022, 2126, 2205, 2562, 2712
Bme18I GGWCC 5 cut(s) 88, 802, 1128, 1305, 3050
BmeT110I CYCGRG 4 cut(s) 1346, 1910, 2639, 3062
BmgT120I GGNCC 9 cut(s) 88, 419, 640, 802, 1128, 1305, 1570, 2642, 3050
BmiI GGNNCC 3 cut(s) 90, 1129, 1849
BmrFI CCNGG 7 cut(s) 677, 1661, 2022, 2126, 2205, 2562, 2712
BpiI GAAGAC 2 cut(s) 1460, 1519
BpmI CTGGAG 4 cut(s) 1143, 1971, 2105, 2256
Bpu10I CCTNAGC 2 cut(s) 1301, 1336
Bpu1102I GCTNAGC 2 cut(s) 240, 363
Bpu14I TTCGAA 2 cut(s) 65, 2894
BpuEI CTTGAG 3 cut(s) 485, 520, 1511
BsaAI YACGTR 1 cut(s) 655
BsaHI GRCGYC 1 cut(s) 317
BsaI GGTCTC 3 cut(s) 666, 2047, 2049
BsaJI CCNNGG 3 cut(s) 135, 2020, 2125
Bsc4I CCNNNNNNNGG 3 cut(s) 2204, 2210, 2632
Bse118I RCCGGY 1 cut(s) 416
Bse1I ACTGG 9 cut(s) 82, 549, 598, 1068, 1465, 1988, 2088, 2239, 2537
Bse3DI GCAATG 4 cut(s) 1287, 1560, 2557, 2798
BseBI CCWGG 7 cut(s) 677, 1661, 2022, 2126, 2205, 2562, 2712
BseDI CCNNGG 3 cut(s) 135, 2020, 2125
BseLI CCNNNNNNNGG 3 cut(s) 2204, 2210, 2632
BseMI GCAATG 4 cut(s) 1287, 1560, 2557, 2798
BseMII CTCAG 6 cut(s) 231, 852, 1350, 1641, 2053, 2330
BseNI ACTGG 9 cut(s) 82, 549, 598, 1068, 1465, 1988, 2088, 2239, 2537
BseRI GAGGAG 1 cut(s) 1103
BseXI GCAGC 7 cut(s) 156, 346, 615, 1271, 2139, 2260, 2786
BsgI GTGCAG 2 cut(s) 2044, 2247
BsiHKAI GWGCWC 3 cut(s) 1277, 1347, 2640
BsiHKCI CYCGRG 4 cut(s) 1346, 1910, 2639, 3062
BsiSI CCGG 1 cut(s) 417
BslFI GGGAC 3 cut(s) 88, 604, 716
BslI CCNNNNNNNGG 3 cut(s) 2204, 2210, 2632
BsmBI CGTCTC 3 cut(s) 1783, 1913, 2126
BsmFI GGGAC 3 cut(s) 88, 604, 716
Bso31I GGTCTC 3 cut(s) 666, 2047, 2049
BsoBI CYCGRG 4 cut(s) 1346, 1910, 2639, 3062
Bsp119I TTCGAA 2 cut(s) 65, 2894
Bsp1286I GDGCHC 4 cut(s) 1277, 1347, 2640, 2891
Bsp1407I TGTACA 1 cut(s) 1857
Bsp1720I GCTNAGC 2 cut(s) 240, 363
BspACI CCGC 8 cut(s) 244, 1213, 1403, 2159, 2211, 2389, 2557, 2920
BspCNI CTCAG 6 cut(s) 232, 851, 1349, 1642, 2052, 2329
BspHI TCATGA 1 cut(s) 1033
BspLI GGNNCC 3 cut(s) 90, 1129, 1849
BspMAI CTGCAG 2 cut(s) 1699, 2776
BspMI ACCTGC 2 cut(s) 19, 504
BspPI GGATC 1 cut(s) 2722
BspT104I TTCGAA 2 cut(s) 65, 2894
BspTNI GGTCTC 3 cut(s) 666, 2047, 2049
BsrBI CCGCTC 2 cut(s) 244, 1215
BsrDI GCAATG 4 cut(s) 1287, 1560, 2557, 2798
BsrFI RCCGGY 1 cut(s) 416
BsrGI TGTACA 1 cut(s) 1857
BsrI ACTGG 9 cut(s) 82, 549, 598, 1068, 1465, 1988, 2088, 2239, 2537
BssAI RCCGGY 1 cut(s) 416
BssECI CCNNGG 3 cut(s) 135, 2020, 2125
BssNI GRCGYC 1 cut(s) 317
BssT1I CCWWGG 1 cut(s) 135
Bst2UI CCWGG 7 cut(s) 677, 1661, 2022, 2126, 2205, 2562, 2712
Bst4CI ACNGT 5 cut(s) 402, 562, 1678, 1873, 1907
Bst6I CTCTTC 5 cut(s) 287, 840, 1145, 2384, 3046
BstACI GRCGYC 1 cut(s) 317
BstAPI GCANNNNNTGC 1 cut(s) 1646
BstAUI TGTACA 1 cut(s) 1857
BstBAI YACGTR 1 cut(s) 655
BstBI TTCGAA 2 cut(s) 65, 2894
BstC8I GCNNGC 9 cut(s) 418, 483, 1584, 1634, 1638, 1642, 1884, 2271, 2646
BstHHI GCGC 1 cut(s) 2370
BstNI CCWGG 7 cut(s) 677, 1661, 2022, 2126, 2205, 2562, 2712
BstNSI RCATGY 8 cut(s) 191, 687, 752, 1586, 1636, 1644, 2226, 2576
BstSCI CCNGG 7 cut(s) 675, 1659, 2020, 2124, 2203, 2560, 2710
BstSFI CTRYAG 3 cut(s) 1695, 2479, 2772
BstV1I GCAGC 7 cut(s) 156, 346, 615, 1271, 2139, 2260, 2786
BstV2I GAAGAC 2 cut(s) 1460, 1519
BstX2I RGATCY 3 cut(s) 889, 2035, 2899
BstYI RGATCY 3 cut(s) 889, 2035, 2899
BsuI GTATCC 2 cut(s) 1087, 2737
BtsI GCAGTG 1 cut(s) 1275
BtsIMutI CAGTG 2 cut(s) 556, 1275
BveI ACCTGC 2 cut(s) 19, 504
Cac8I GCNNGC 9 cut(s) 418, 483, 1584, 1634, 1638, 1642, 1884, 2271, 2646
CaiI CAGNNNCTG 1 cut(s) 2234
CciI TCATGA 1 cut(s) 1033
CfoI GCGC 1 cut(s) 2370
Cfr10I RCCGGY 1 cut(s) 416
Cfr13I GGNCC 9 cut(s) 88, 419, 640, 802, 1128, 1305, 1570, 2642, 3050
CseI GACGC 4 cut(s) 42, 306, 2556, 2708
CsiI ACCWGGT 1 cut(s) 675
Csp6I GTAC 7 cut(s) 496, 517, 650, 1700, 1858, 2871, 3013
CviQI GTAC 7 cut(s) 496, 517, 650, 1700, 1858, 2871, 3013
EaeI YGGCCR 3 cut(s) 2195, 2430, 3000
Eam1104I CTCTTC 5 cut(s) 287, 840, 1145, 2384, 3046
EarI CTCTTC 5 cut(s) 287, 840, 1145, 2384, 3046
EciI GGCGGA 2 cut(s) 1418, 2226
Ecl136II GAGCTC 2 cut(s) 1345, 2638
Eco130I CCWWGG 1 cut(s) 135
Eco147I AGGCCT 1 cut(s) 1882
Eco24I GRGCYC 3 cut(s) 1347, 2640, 2891
Eco31I GGTCTC 3 cut(s) 666, 2047, 2049
Eco32I GATATC 2 cut(s) 198, 885
Eco47I GGWCC 5 cut(s) 88, 802, 1128, 1305, 3050
Eco53kI GAGCTC 2 cut(s) 1345, 2638
Eco57I CTGAAG 5 cut(s) 41, 252, 727, 2700, 3027
Eco88I CYCGRG 4 cut(s) 1346, 1910, 2639, 3062
EcoICRI GAGCTC 2 cut(s) 1345, 2638
EcoO109I RGGNCCY 2 cut(s) 1128, 1305
EcoRI GAATTC 3 cut(s) 67, 539, 1801
EcoRII CCWGG 7 cut(s) 675, 1659, 2020, 2124, 2203, 2560, 2710
EcoRV GATATC 2 cut(s) 198, 885
EcoT14I CCWWGG 1 cut(s) 135
EcoT22I ATGCAT 1 cut(s) 1634
EcoT38I GRGCYC 3 cut(s) 1347, 2640, 2891
ErhI CCWWGG 1 cut(s) 135
Esp3I CGTCTC 3 cut(s) 1783, 1913, 2126
FalI AAGNNNNNCTT 4 cut(s) 1423, 1455, 1803, 1835
FaqI GGGAC 3 cut(s) 88, 604, 716
FauNDI CATATG 1 cut(s) 532
Fnu4HI GCNGC 8 cut(s) 170, 360, 629, 1260, 2153, 2274, 2558, 2775
FriOI GRGCYC 3 cut(s) 1347, 2640, 2891
FseI GGCCGGCC 1 cut(s) 420
Fsp4HI GCNGC 8 cut(s) 170, 360, 629, 1260, 2153, 2274, 2558, 2775
FspBI CTAG 7 cut(s) 753, 1008, 1022, 1703, 1878, 2544, 2661
GlaI GCGC 1 cut(s) 2369
GluI GCNGC 8 cut(s) 170, 360, 629, 1260, 2153, 2274, 2558, 2775
GsuI CTGGAG 4 cut(s) 1143, 1971, 2105, 2256
HapII CCGG 1 cut(s) 417
HgaI GACGC 4 cut(s) 42, 306, 2556, 2708
HhaI GCGC 1 cut(s) 2370
Hin1I GRCGYC 1 cut(s) 317
Hin6I GCGC 1 cut(s) 2368
HinP1I GCGC 1 cut(s) 2368
HincII GTYRAC 2 cut(s) 1162, 2530
HindII GTYRAC 2 cut(s) 1162, 2530
HindIII AAGCTT 4 cut(s) 45, 159, 1529, 1750
HpaII CCGG 1 cut(s) 417
HphI GGTGA 7 cut(s) 92, 286, 691, 1070, 1159, 1753, 2709
Hpy166II GTNNAC 9 cut(s) 8, 57, 652, 802, 1061, 1162, 1199, 2530, 2965
Hpy188III TCNNGA 8 cut(s) 346, 895, 986, 1022, 1034, 1122, 2620, 2678
Hpy8I GTNNAC 9 cut(s) 8, 57, 652, 802, 1061, 1162, 1199, 2530, 2965
HpyCH4III ACNGT 5 cut(s) 402, 562, 1678, 1873, 1907
HpyCH4IV ACGT 7 cut(s) 372, 654, 772, 1377, 2334, 2961, 2967
HpySE526I ACGT 7 cut(s) 372, 654, 772, 1377, 2334, 2961, 2967
Hsp92I GRCGYC 1 cut(s) 317
HspAI GCGC 1 cut(s) 2368
KroI GCCGGC 1 cut(s) 416
KroNI GCCGGC 1 cut(s) 418
LmnI GCTCC 6 cut(s) 391, 1113, 2086, 2635, 2680, 3027
Lsp1109I GCAGC 7 cut(s) 156, 346, 615, 1271, 2139, 2260, 2786
MabI ACCWGGT 1 cut(s) 675
MaeI CTAG 7 cut(s) 753, 1008, 1022, 1703, 1878, 2544, 2661
MaeII ACGT 7 cut(s) 372, 654, 772, 1377, 2334, 2961, 2967
MaeIII GTNAC 5 cut(s) 679, 1382, 1456, 1678, 2135
MbiI CCGCTC 2 cut(s) 244, 1215
MfeI CAATTG 1 cut(s) 2309
MflI RGATCY 3 cut(s) 889, 2035, 2899
MhlI GDGCHC 4 cut(s) 1277, 1347, 2640, 2891
MlsI TGGCCA 2 cut(s) 2432, 3002
MluNI TGGCCA 2 cut(s) 2432, 3002
MlyI GAGTC 2 cut(s) 13, 1157
MmeI TCCRAC 1 cut(s) 2213
Mox20I TGGCCA 2 cut(s) 2432, 3002
Mph1103I ATGCAT 1 cut(s) 1634
MroNI GCCGGC 1 cut(s) 416
MroXI GAANNNNTTC 1 cut(s) 981
MscI TGGCCA 2 cut(s) 2432, 3002
MslI CAYNNNNRTG 4 cut(s) 922, 1038, 1280, 2073
Msp20I TGGCCA 2 cut(s) 2432, 3002
MspI CCGG 1 cut(s) 417
MspR9I CCNGG 7 cut(s) 677, 1661, 2022, 2126, 2205, 2562, 2712
MunI CAATTG 1 cut(s) 2309
MvaI CCWGG 7 cut(s) 677, 1661, 2022, 2126, 2205, 2562, 2712
NaeI GCCGGC 1 cut(s) 418
NdeI CATATG 1 cut(s) 532
NgoMIV GCCGGC 1 cut(s) 416
NlaIV GGNNCC 3 cut(s) 90, 1129, 1849
NmeAIII GCCGAG 2 cut(s) 1366, 1871
NmuCI GTSAC 2 cut(s) 679, 1678
NsiI ATGCAT 1 cut(s) 1634
NspI RCATGY 8 cut(s) 191, 687, 752, 1586, 1636, 1644, 2226, 2576
NspV TTCGAA 2 cut(s) 65, 2894
PaeI GCATGC 3 cut(s) 1586, 1636, 1644
PaeR7I CTCGAG 1 cut(s) 1346
PagI TCATGA 1 cut(s) 1033
PaqCI CACCTGC 1 cut(s) 19
PceI AGGCCT 1 cut(s) 1882
PciI ACATGT 3 cut(s) 187, 2222, 2572
PcsI WCGNNNNNNNCGW 1 cut(s) 1383
PdiI GCCGGC 1 cut(s) 418
PdmI GAANNNNTTC 1 cut(s) 981
PfeI GAWTC 8 cut(s) 32, 333, 756, 764, 1045, 1620, 1769, 2990
PflFI GACNNNGTC 1 cut(s) 92
PfoI TCCNGGA 2 cut(s) 2203, 2710
PkrI GCNGC 8 cut(s) 171, 361, 630, 1261, 2154, 2275, 2559, 2776
PleI GAGTC 2 cut(s) 12, 1157
PpsI GAGTC 2 cut(s) 12, 1157
Ppu21I YACGTR 1 cut(s) 655
PpuMI RGGWCCY 2 cut(s) 1128, 1305
PscI ACATGT 3 cut(s) 187, 2222, 2572
PshBI ATTAAT 1 cut(s) 2286
PsiI TTATAA 3 cut(s) 327, 1082, 2585
Psp124BI GAGCTC 2 cut(s) 1347, 2640
Psp1406I AACGTT 4 cut(s) 372, 772, 1377, 2334
Psp5II RGGWCCY 2 cut(s) 1128, 1305
Psp6I CCWGG 7 cut(s) 675, 1659, 2020, 2124, 2203, 2560, 2710
PspGI CCWGG 7 cut(s) 675, 1659, 2020, 2124, 2203, 2560, 2710
PspN4I GGNNCC 3 cut(s) 90, 1129, 1849
PspPI GGNCC 9 cut(s) 88, 419, 640, 802, 1128, 1305, 1570, 2642, 3050
PspPPI RGGWCCY 2 cut(s) 1128, 1305
PspXI VCTCGAGB 1 cut(s) 1346
PstI CTGCAG 2 cut(s) 1699, 2776
PstNI CAGNNNCTG 1 cut(s) 2234
PsuI RGATCY 3 cut(s) 889, 2035, 2899
PsyI GACNNNGTC 1 cut(s) 92
RigI GGCCGGCC 1 cut(s) 420
RsaI GTAC 7 cut(s) 497, 518, 651, 1701, 1859, 2872, 3014
RsaNI GTAC 7 cut(s) 496, 517, 650, 1700, 1858, 2871, 3013
RseI CAYNNNNRTG 4 cut(s) 922, 1038, 1280, 2073
SacI GAGCTC 2 cut(s) 1347, 2640
SatI GCNGC 8 cut(s) 170, 360, 629, 1260, 2153, 2274, 2558, 2775
Sau96I GGNCC 9 cut(s) 88, 419, 640, 802, 1128, 1305, 1570, 2642, 3050
ScaI AGTACT 2 cut(s) 1701, 2872
SchI GAGTC 2 cut(s) 13, 1157
ScrFI CCNGG 7 cut(s) 677, 1661, 2022, 2126, 2205, 2562, 2712
SduI GDGCHC 4 cut(s) 1277, 1347, 2640, 2891
SexAI ACCWGGT 1 cut(s) 675
SfcI CTRYAG 3 cut(s) 1695, 2479, 2772
Sfr274I CTCGAG 1 cut(s) 1346
SfuI TTCGAA 2 cut(s) 65, 2894
SinI GGWCC 5 cut(s) 88, 802, 1128, 1305, 3050
SlaI CTCGAG 1 cut(s) 1346
SmiMI CAYNNNNRTG 4 cut(s) 922, 1038, 1280, 2073
SmlI CTYRAG 4 cut(s) 464, 499, 1346, 1526
SmoI CTYRAG 4 cut(s) 464, 499, 1346, 1526
SphI GCATGC 3 cut(s) 1586, 1636, 1644
SseBI AGGCCT 1 cut(s) 1882
SsiI CCGC 8 cut(s) 244, 1213, 1403, 2159, 2211, 2389, 2557, 2920
SspI AATATT 1 cut(s) 949
SspMI CTAG 7 cut(s) 753, 1008, 1022, 1703, 1878, 2544, 2661
SstI GAGCTC 2 cut(s) 1347, 2640
StuI AGGCCT 1 cut(s) 1882
StyD4I CCNGG 7 cut(s) 675, 1659, 2020, 2124, 2203, 2560, 2710
StyI CCWWGG 1 cut(s) 135
TaaI ACNGT 5 cut(s) 402, 562, 1678, 1873, 1907
TaiI ACGT 7 cut(s) 375, 657, 775, 1380, 2337, 2964, 2970
TaqI TCGA 7 cut(s) 65, 103, 762, 1347, 2298, 2894, 2949
TatI WGTACW 4 cut(s) 495, 1699, 1857, 2870
TauI GCSGC 1 cut(s) 2560
TfiI GAWTC 8 cut(s) 32, 333, 756, 764, 1045, 1620, 1769, 2990
TscAI CASTG 2 cut(s) 556, 1282
TseFI GTSAC 2 cut(s) 679, 1678
TseI GCWGC 7 cut(s) 169, 359, 628, 1259, 2152, 2273, 2774
Tsp45I GTSAC 2 cut(s) 679, 1678
TspDTI ATGAA 8 cut(s) 195, 716, 948, 1005, 1744, 1779, 1794, 2613
TspGWI ACGGA 1 cut(s) 56
TspRI CASTG 2 cut(s) 556, 1282
Tth111I GACNNNGTC 1 cut(s) 92
VpaK11BI GGWCC 5 cut(s) 88, 802, 1128, 1305, 3050
VspI ATTAAT 1 cut(s) 2286
XapI RAATTY 7 cut(s) 67, 202, 487, 539, 1135, 1801, 2092
XbaI TCTAGA 1 cut(s) 1021
XceI RCATGY 8 cut(s) 191, 687, 752, 1586, 1636, 1644, 2226, 2576
XhoI CTCGAG 1 cut(s) 1346
XmnI GAANNNNTTC 1 cut(s) 981
XspI CTAG 7 cut(s) 753, 1008, 1022, 1703, 1878, 2544, 2661
ZrmI AGTACT 2 cut(s) 1701, 2872
Zsp2I ATGCAT 1 cut(s) 1634
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.