Rmu_sc0000129.1_g000005

Retinoblastoma-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000129.1
OG0003674
Physical Location & Seq
Forward (+)
23450 .. 35571
12122 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000129.1_g000005.1.cds

Sequence Viewer

Length: 3567 bp
atggaagatacgaagccggaggtctctgcatccaacaattcgaattcgggaaccggagatttggaccctgttaaagctcgatttactgatttgtgcaagaacggattgtcgttggacgagaacacgctgacgcaagctatcaagctgctgatagaaacgaaacatgttctaatatcaaatttatcggctatcggaaatggaacgcctgaagaagcagagcggttctggttttcgtttgttttgttctcggtgaaaacattgagtgagaagagttctgataattcgcaaaaggggtctgatgataatggattctctctcatccaaattttgagagctgctaagctgaacattgtggatttctttaaggagcttccacagtttattgttaaggccggcccaattttaagtaatctgtatggatttatcggctatcggaaatggaacggtaatcctgaagaagcagagcggttctggttttcgtttgttttgttctcggtgaaaacattgagtgagaagagttctgataattcgcaaaaggggtctgatgataatggattctctctcatccaaattttgagagctgctaagctgaacattgtggatttctttaaggagcttccacagtttattgttaaggccggcccaattttaagtaatctgtatggcgtagattgggagagcaaacttgaggcaaaagagttgcaggccaattttctgtacttgagccttttaagcaagtacttcaaaaaagcataccgggaattcttcttgacaagtgatgcaactgttgataagaagtcagccattaccaatggagctggctatgtgtcagagtatcatagatttggatggctgctgtttttggccctccgggtacatgtatttgggcgagttaaagacctggtgacatgcacaaacggcttggtgtccatattggcaattttaattattcacgtccctgttcgcttcagaaactttagaatccatgattcaccgcgttttgtgaggaaaggcaacaaaggtgtggacctgcttgcatcactttgcaacacttatgacacctcagaagaggagttgagaaaaacaatggaaaaggctaatacattgatatcagatcttttgaagaagaagccttgtttggcatctgagtgtaaaaatgaaaatctaaagaatattgatacagatggtttgacctattttgaaggacttctggatgaaccttctttgtcatccagtttagatattctagaaaaagatcatgatgatgcaattcggaataagggtgaactggatgagagagtatttataaatgaggaggacagcttacttggttcagggagcttgtcaggagggtccttgaatttatctggtgtcaagaggaaatttgattcaatggcctccccaacaaagacaattacaagtccactctctccccatcgctctccagcctcgcatgttaatggtggtgttaattcaaagatggcagctacacctgtgagcactgcaatgacaactgcaaagtggcttaggacctttatttccccacttccagatgagagagtatttataaatgaggaggacagcttacttggttcagggagcttgtcaggagggtccttgaatttatctggtgtgaagaggaaatttgattcaatggcctccccaacaaagacaattacaagtccactctctccccatcgctctccagcctcgcatgttaatggtggtgttaattcaaagatggcagctacacctgtgagcactgcaatgacaactgcaaagtggcttaggacctttatttccccacttccgtcaaaaccctcacctgagcttgagggttttctggcatcgtgtgataggaacgttagtaatgatgtgatacgcagggcgcaaataatacttgaggccatatttccaagtagtgctttgggggagcgatgtgtaactggtagtctgcaaagtgctaacctcatggacaatgtgtgggcagaacaacgaagactggaagcactcaagctttattatagagttttggaagcaatgtgtagggcagaggctcaagttctgcatgctactaatttgacctctttattaacaaatgagaggttccatagatgcatgctagcatgctctgctgagttagtcctggcaacacataagacggtcacaatgttgtttcctgccgtactagaaaggacaggcattacagcttttgatcttagcaaggtgatagagagcttcattagacatgaagaatctctaccaagagaattgagacgacatctaaattcattggaagaacgacttttggagagcatggtatgggagaagggttcctcaatgtacaattctttgacagttgctaggcctgccttctcggctgaaatcaaccgtctcgggttattagcagaaccaatgccatctttggatgcaatagctttgcatatcaacttctcttgtggagggttgcctccagtagcttctttacagaagcatgagacttccccaggtcagaatggagatctcaggtctccgaagagaccctgcacagaatataggggtgtattactggagcgaaattcctttacttctccagtgaaggatcgtctcctggccttaagtaaccttaaatcaaagctgccaccaccacctctacaatctgcatttgcaagtccaacacgaccaaatccaggaggtggaggggaaacatgtgcagaaactggagttagtattttctttaataagatcatcaagctggctgctgtaagaattaatggtatggtcgaaaggttgcaattgtctcagcaaatcagggagaatgtttatcgcctcttccaacaaatacttgttcagcggacatctctcttttttaaccgccatattgatcaaattatcctttgttgtttttacggggttgccaagatttctcaactaagtcttacatttagggaaatcatacacaactacaggaagcaaccacaatgcaaaccacaagtttttcgaagtgtctttgttgactggtcatcagctagacgcaatgggcggcctggacaggaacacgtagatatcattagattttacaatgaagtattcattccttctgtcaagaccttgcttgttgagcttgggccagctgttacaactacaagagccaatgtagttcctgaagctaacaacaacaatgatgctcaatgtccaggatcacctaaagtatccacctttccaagtctccctgatatgtctccaaagaaagtatctgcagcacacaatgtttatgtatctccattgcgatcatcaaagatggatgccttaatctcacatagctcaaagagctattatgcttgtgttggggagagtactcatgcgtatcagagcccttcaaaagatctgacagccattaacaaccgcttgaatggcacacgaaaacgcacactcgactttgataatgtggatgtaggggttggtttggttagtgattctatggtggccaatagcctgtaccttcaaaatgggagttgtgcatcctcatcgggtggaccattgaaaaccgagcaacctgacttgtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000082 GO:0000278 GO:0000280 GO:0000902 GO:0000904 GO:0001558 GO:0001708 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006275 GO:0006349 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007059 GO:0007127 GO:0007129 GO:0007275 GO:0008134 GO:0008150 GO:0008152 GO:0008356 GO:0009553 GO:0009555 GO:0009566 GO:0009567 GO:0009653 GO:0009791 GO:0009888 GO:0009889 GO:0009987 GO:0010016 GO:0010026 GO:0010052 GO:0010090 GO:0010103 GO:0010374 GO:0010377 GO:0010468 GO:0010556 GO:0010564 GO:0016043 GO:0019219 GO:0019222 GO:0019953 GO:0022402 GO:0022414 GO:0022619 GO:0030154 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032875 GO:0032989 GO:0033043 GO:0034641 GO:0040008 GO:0040029 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0044770 GO:0044772 GO:0044843 GO:0045132 GO:0045143 GO:0045165 GO:0046483 GO:0048229 GO:0048285 GO:0048366 GO:0048367 GO:0048468 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051052 GO:0051128 GO:0051171 GO:0051239 GO:0051276 GO:0051301 GO:0051321 GO:0051704 GO:0051726 GO:0051783 GO:0055046 GO:0060255 GO:0061982 GO:0065007 GO:0070192 GO:0071514 GO:0071704 GO:0071840 GO:0080090 GO:0090329 GO:0090558 GO:0090626 GO:0090627 GO:0090698 GO:0097159 GO:0098813 GO:0099402 GO:0140013 GO:1901360 GO:1901363 GO:1903046 GO:1903047 GO:1903866 GO:2000036 GO:2000112 GO:2000653
KEGG Pathways
Metabolic & Signaling
ko04110 ko04218 ko04350 ko05165 ko05203 map04110 map04218 map04350 map05165 map05203
Pfam Domains
Protein Families
DUF3452 RB_A RB_B Rb_C

Protein Analysis

1188

Amino Acids

131.32

Weight (kDa)

8.85

Isoelectric Point (pI)

47.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1307, 1568
Acc36I ACCTGC 1 cut(s) 1038
AccB7I CCANNNNNTGG 1 cut(s) 2696
AccBSI CCGCTC 2 cut(s) 220, 466
AccII CGCG 1 cut(s) 997
AciI CCGC 7 cut(s) 220, 466, 995, 2854, 2875, 3043, 3406
AclI AACGTT 1 cut(s) 1863
AclWI GGATC 2 cut(s) 2610, 3208
AcoI YGGCCR 1 cut(s) 3486
AcuI CTGAAG 4 cut(s) 228, 474, 952, 3186
AfaI GTAC 7 cut(s) 719, 740, 876, 2187, 2345, 3358, 3500
AfiI CCNNNNNNNGG 2 cut(s) 2690, 2696
AflII CTTAAG 1 cut(s) 2617
AflIII ACRYGT 4 cut(s) 163, 877, 2708, 3058
AjiI CACGTC 1 cut(s) 955
AjnI CCWGG 7 cut(s) 900, 2145, 2506, 2610, 2689, 3046, 3196
AjuI GAANNNNNNNTTGG 2 cut(s) 1121, 1153
Alw21I GWGCWC 2 cut(s) 1502, 1763
AlwI GGATC 2 cut(s) 2610, 3208
AlwNI CAGNNNCTG 1 cut(s) 2720
Ama87I CYCGRG 1 cut(s) 2396
ApeKI GCWGC 9 cut(s) 145, 335, 581, 853, 1484, 1745, 2638, 2759, 3260
AseI ATTAAT 1 cut(s) 2772
Asp700I GAANNNNTTC 1 cut(s) 1206
AspLEI GCGC 1 cut(s) 1891
AsuC2I CCSGG 2 cut(s) 758, 872
AsuHPI GGTGA 8 cut(s) 262, 508, 916, 984, 1295, 1815, 2239, 3195
AsuII TTCGAA 2 cut(s) 41, 3001
AsuNHI GCTAGC 1 cut(s) 2122
AvaI CYCGRG 1 cut(s) 2396
AvaII GGWCC 7 cut(s) 64, 1027, 1353, 1530, 1614, 1791, 3536
BalI TGGCCA 1 cut(s) 3488
BanII GRGCYC 1 cut(s) 3377
BarI GAAGNNNNNNTAC 2 cut(s) 2244, 2276
BbsI GAAGAC 1 cut(s) 2005
Bbv12I GWGCWC 2 cut(s) 1502, 1763
BbvI GCAGC 9 cut(s) 132, 322, 568, 840, 1496, 1757, 2625, 2746, 3272
BccI CCATC 8 cut(s) 843, 1178, 1443, 1474, 1704, 1735, 2428, 3295
BceAI ACGGC 2 cut(s) 934, 2168
BcgI CGANNNNNNTGC 2 cut(s) 3510, 3544
BciT130I CCWGG 7 cut(s) 902, 2147, 2508, 2612, 2691, 3048, 3198
BciVI GTATCC 1 cut(s) 3223
BclI TGATCA 1 cut(s) 2884
BcnI CCSGG 2 cut(s) 758, 872
BfaI CTAG 5 cut(s) 1247, 2123, 2189, 2364, 3030
BfmI CTRYAG 2 cut(s) 2965, 3258
BfrI CTTAAG 1 cut(s) 2617
BfuAI ACCTGC 1 cut(s) 1038
BfuI GTATCC 1 cut(s) 3223
BglI GCCNNNNNGGC 1 cut(s) 2378
BglII AGATCT 3 cut(s) 1114, 2521, 3385
BlpI GCTNAGC 2 cut(s) 339, 585
BmcAI AGTACT 2 cut(s) 740, 3358
Bme1390I CCNGG 9 cut(s) 758, 872, 902, 2147, 2508, 2612, 2691, 3048, 3198
Bme18I GGWCC 7 cut(s) 64, 1027, 1353, 1530, 1614, 1791, 3536
BmeT110I CYCGRG 1 cut(s) 2396
BmgBI CACGTC 1 cut(s) 955
BmiI GGNNCC 6 cut(s) 52, 66, 1354, 1615, 2108, 2335
BmrFI CCNGG 9 cut(s) 758, 872, 902, 2147, 2508, 2612, 2691, 3048, 3198
BmtI GCTAGC 1 cut(s) 2126
BpiI GAAGAC 1 cut(s) 2005
BpmI CTGGAG 6 cut(s) 1428, 1689, 2457, 2577, 2591, 2742
Bpu10I CCTNAGC 3 cut(s) 1526, 1787, 1827
Bpu1102I GCTNAGC 2 cut(s) 339, 585
Bpu14I TTCGAA 2 cut(s) 41, 3001
BpuEI CTTGAG 6 cut(s) 707, 742, 1853, 1922, 1997, 2043
BpuMI CCSGG 2 cut(s) 758, 872
BsaAI YACGTR 1 cut(s) 3061
BsaI GGTCTC 3 cut(s) 28, 2533, 2535
BsaJI CCNNGG 1 cut(s) 2506
BsaWI WCCGGW 1 cut(s) 53
Bsc4I CCNNNNNNNGG 2 cut(s) 2690, 2696
Bse118I RCCGGY 2 cut(s) 392, 638
Bse1I ACTGG 9 cut(s) 1233, 1293, 1951, 2007, 2474, 2574, 2594, 2725, 3023
Bse3DI GCAATG 5 cut(s) 1512, 1773, 2046, 3043, 3284
BseBI CCWGG 7 cut(s) 902, 2147, 2508, 2612, 2691, 3048, 3198
BseDI CCNNGG 1 cut(s) 2506
BseLI CCNNNNNNNGG 2 cut(s) 2690, 2696
BseMI GCAATG 5 cut(s) 1512, 1773, 2046, 3043, 3284
BseMII CTCAG 6 cut(s) 1077, 1137, 1818, 2127, 2539, 2816
BseNI ACTGG 9 cut(s) 1233, 1293, 1951, 2007, 2474, 2574, 2594, 2725, 3023
BseRI GAGGAG 3 cut(s) 1085, 1328, 1589
BseXI GCAGC 9 cut(s) 132, 322, 568, 840, 1496, 1757, 2625, 2746, 3272
BsgI GTGCAG 2 cut(s) 2530, 2733
Bsh1236I CGCG 1 cut(s) 997
BsiHKAI GWGCWC 2 cut(s) 1502, 1763
BsiHKCI CYCGRG 1 cut(s) 2396
BsiSI CCGG 6 cut(s) 17, 54, 393, 639, 757, 871
BslFI GGGAC 1 cut(s) 941
BslI CCNNNNNNNGG 2 cut(s) 2690, 2696
BsmBI CGTCTC 3 cut(s) 2269, 2399, 2612
BsmFI GGGAC 1 cut(s) 941
Bso31I GGTCTC 3 cut(s) 28, 2533, 2535
BsoBI CYCGRG 1 cut(s) 2396
Bsp119I TTCGAA 2 cut(s) 41, 3001
Bsp1286I GDGCHC 3 cut(s) 1502, 1763, 3377
Bsp1407I TGTACA 1 cut(s) 2343
Bsp1720I GCTNAGC 2 cut(s) 339, 585
BspACI CCGC 7 cut(s) 220, 466, 995, 2854, 2875, 3043, 3406
BspCNI CTCAG 6 cut(s) 1076, 1138, 1819, 2128, 2538, 2815
BspFNI CGCG 1 cut(s) 997
BspHI TCATGA 1 cut(s) 1258
BspLI GGNNCC 6 cut(s) 52, 66, 1354, 1615, 2108, 2335
BspMAI CTGCAG 1 cut(s) 3262
BspMI ACCTGC 1 cut(s) 1038
BspOI GCTAGC 1 cut(s) 2126
BspPI GGATC 2 cut(s) 2610, 3208
BspT104I TTCGAA 2 cut(s) 41, 3001
BspTI CTTAAG 1 cut(s) 2617
BspTNI GGTCTC 3 cut(s) 28, 2533, 2535
BsrBI CCGCTC 2 cut(s) 220, 466
BsrDI GCAATG 5 cut(s) 1512, 1773, 2046, 3043, 3284
BsrFI RCCGGY 2 cut(s) 392, 638
BsrGI TGTACA 1 cut(s) 2343
BsrI ACTGG 9 cut(s) 1233, 1293, 1951, 2007, 2474, 2574, 2594, 2725, 3023
BssAI RCCGGY 2 cut(s) 392, 638
BssECI CCNNGG 1 cut(s) 2506
Bst2UI CCWGG 7 cut(s) 902, 2147, 2508, 2612, 2691, 3048, 3198
Bst4CI ACNGT 7 cut(s) 378, 446, 624, 787, 2164, 2359, 2393
Bst6I CTCTTC 6 cut(s) 263, 509, 1062, 1631, 2531, 2837
BstAFI CTTAAG 1 cut(s) 2617
BstAPI GCANNNNNTGC 1 cut(s) 2132
BstAUI TGTACA 1 cut(s) 2343
BstBAI YACGTR 1 cut(s) 3061
BstBI TTCGAA 2 cut(s) 41, 3001
BstFNI CGCG 1 cut(s) 997
BstHHI GCGC 1 cut(s) 1891
BstMWI GCNNNNNNNGC 8 cut(s) 732, 918, 2132, 2369, 2378, 3121, 3330, 3414
BstNI CCWGG 7 cut(s) 902, 2147, 2508, 2612, 2691, 3048, 3198
BstNSI RCATGY 9 cut(s) 167, 881, 912, 1457, 1718, 2072, 2122, 2130, 2712
BstSCI CCNGG 9 cut(s) 756, 870, 900, 2145, 2506, 2610, 2689, 3046, 3196
BstSFI CTRYAG 2 cut(s) 2965, 3258
BstUI CGCG 1 cut(s) 997
BstV1I GCAGC 9 cut(s) 132, 322, 568, 840, 1496, 1757, 2625, 2746, 3272
BstV2I GAAGAC 1 cut(s) 2005
BstX2I RGATCY 3 cut(s) 1114, 2521, 3385
BstYI RGATCY 3 cut(s) 1114, 2521, 3385
BsuI GTATCC 1 cut(s) 3223
BtgZI GCGATG 3 cut(s) 1421, 1682, 1951
BtrI CACGTC 1 cut(s) 955
BtsI GCAGTG 2 cut(s) 1500, 1761
BtsIMutI CAGTG 3 cut(s) 1500, 1761, 2601
BveI ACCTGC 1 cut(s) 1038
CaiI CAGNNNCTG 1 cut(s) 2720
CciI TCATGA 1 cut(s) 1258
CfoI GCGC 1 cut(s) 1891
Cfr10I RCCGGY 2 cut(s) 392, 638
CseI GACGC 2 cut(s) 139, 3042
CsiI ACCWGGT 1 cut(s) 900
Csp6I GTAC 7 cut(s) 718, 739, 875, 2186, 2344, 3357, 3499
CviQI GTAC 7 cut(s) 718, 739, 875, 2186, 2344, 3357, 3499
EaeI YGGCCR 1 cut(s) 3486
Eam1104I CTCTTC 6 cut(s) 263, 509, 1062, 1631, 2531, 2837
EarI CTCTTC 6 cut(s) 263, 509, 1062, 1631, 2531, 2837
Eco147I AGGCCT 1 cut(s) 2368
Eco24I GRGCYC 1 cut(s) 3377
Eco31I GGTCTC 3 cut(s) 28, 2533, 2535
Eco32I GATATC 2 cut(s) 1110, 3067
Eco47I GGWCC 7 cut(s) 64, 1027, 1353, 1530, 1614, 1791, 3536
Eco57I CTGAAG 4 cut(s) 228, 474, 952, 3186
Eco88I CYCGRG 1 cut(s) 2396
EcoO109I RGGNCCY 4 cut(s) 1353, 1530, 1614, 1791
EcoRI GAATTC 2 cut(s) 43, 761
EcoRII CCWGG 7 cut(s) 900, 2145, 2506, 2610, 2689, 3046, 3196
EcoRV GATATC 2 cut(s) 1110, 3067
EcoT22I ATGCAT 1 cut(s) 2120
EcoT38I GRGCYC 1 cut(s) 3377
Esp3I CGTCTC 3 cut(s) 2269, 2399, 2612
FalI AAGNNNNNCTT 4 cut(s) 1909, 1941, 2289, 2321
FaqI GGGAC 1 cut(s) 941
FbaI TGATCA 1 cut(s) 2884
FriOI GRGCYC 1 cut(s) 3377
FseI GGCCGGCC 2 cut(s) 396, 642
FspBI CTAG 5 cut(s) 1247, 2123, 2189, 2364, 3030
GlaI GCGC 1 cut(s) 1890
GsuI CTGGAG 6 cut(s) 1428, 1689, 2457, 2577, 2591, 2742
HapII CCGG 6 cut(s) 17, 54, 393, 639, 757, 871
HgaI GACGC 2 cut(s) 139, 3042
HhaI GCGC 1 cut(s) 1891
Hin6I GCGC 1 cut(s) 1889
HinP1I GCGC 1 cut(s) 1889
HincII GTYRAC 1 cut(s) 3016
HindII GTYRAC 1 cut(s) 3016
HindIII AAGCTT 1 cut(s) 2015
HinfI GANTC 8 cut(s) 309, 555, 981, 989, 1388, 1649, 2255, 3476
HpaII CCGG 6 cut(s) 17, 54, 393, 639, 757, 871
HphI GGTGA 8 cut(s) 262, 508, 916, 984, 1295, 1815, 2239, 3195
Hpy166II GTNNAC 6 cut(s) 1027, 1286, 1424, 1685, 3016, 3536
Hpy8I GTNNAC 6 cut(s) 1027, 1286, 1424, 1685, 3016, 3536
HpyAV CCTTC 8 cut(s) 1196, 1230, 2323, 2383, 2593, 3108, 3387, 3512
HpyCH4III ACNGT 7 cut(s) 378, 446, 624, 787, 2164, 2359, 2393
HpyCH4IV ACGT 3 cut(s) 954, 1863, 3060
HpyF10VI GCNNNNNNNGC 8 cut(s) 732, 918, 2132, 2369, 2378, 3121, 3330, 3414
HpySE526I ACGT 3 cut(s) 954, 1863, 3060
HspAI GCGC 1 cut(s) 1889
KroI GCCGGC 2 cut(s) 392, 638
KroNI GCCGGC 2 cut(s) 394, 640
Ksp22I TGATCA 1 cut(s) 2884
LmnI GCTCC 7 cut(s) 367, 613, 815, 1338, 1599, 1933, 2572
Lsp1109I GCAGC 9 cut(s) 132, 322, 568, 840, 1496, 1757, 2625, 2746, 3272
MabI ACCWGGT 1 cut(s) 900
MaeI CTAG 5 cut(s) 1247, 2123, 2189, 2364, 3030
MaeII ACGT 3 cut(s) 954, 1863, 3060
MaeIII GTNAC 5 cut(s) 904, 1942, 2164, 2621, 3136
MbiI CCGCTC 2 cut(s) 220, 466
MfeI CAATTG 1 cut(s) 2795
MflI RGATCY 3 cut(s) 1114, 2521, 3385
MhlI GDGCHC 3 cut(s) 1502, 1763, 3377
MlsI TGGCCA 1 cut(s) 3488
MluNI TGGCCA 1 cut(s) 3488
MmeI TCCRAC 4 cut(s) 57, 93, 2699, 2860
Mox20I TGGCCA 1 cut(s) 3488
Mph1103I ATGCAT 1 cut(s) 2120
MroNI GCCGGC 2 cut(s) 392, 638
MroXI GAANNNNTTC 1 cut(s) 1206
MscI TGGCCA 1 cut(s) 3488
MslI CAYNNNNRTG 6 cut(s) 1147, 1263, 1458, 1505, 1719, 1766
Msp20I TGGCCA 1 cut(s) 3488
MspA1I CMGCKG 2 cut(s) 2854, 3134
MspCI CTTAAG 1 cut(s) 2617
MspI CCGG 6 cut(s) 17, 54, 393, 639, 757, 871
MspR9I CCNGG 9 cut(s) 758, 872, 902, 2147, 2508, 2612, 2691, 3048, 3198
MunI CAATTG 1 cut(s) 2795
MvaI CCWGG 7 cut(s) 902, 2147, 2508, 2612, 2691, 3048, 3198
MvnI CGCG 1 cut(s) 997
MwoI GCNNNNNNNGC 8 cut(s) 732, 918, 2132, 2369, 2378, 3121, 3330, 3414
NaeI GCCGGC 2 cut(s) 394, 640
NciI CCSGG 2 cut(s) 758, 872
NgoMIV GCCGGC 2 cut(s) 392, 638
NheI GCTAGC 1 cut(s) 2122
NlaIV GGNNCC 6 cut(s) 52, 66, 1354, 1615, 2108, 2335
NmeAIII GCCGAG 1 cut(s) 2357
NmuCI GTSAC 2 cut(s) 904, 2164
NsiI ATGCAT 1 cut(s) 2120
NspI RCATGY 9 cut(s) 167, 881, 912, 1457, 1718, 2072, 2122, 2130, 2712
NspV TTCGAA 2 cut(s) 41, 3001
PaeI GCATGC 3 cut(s) 2072, 2122, 2130
PagI TCATGA 1 cut(s) 1258
PceI AGGCCT 1 cut(s) 2368
PciI ACATGT 3 cut(s) 163, 877, 2708
PdiI GCCGGC 2 cut(s) 394, 640
PdmI GAANNNNTTC 1 cut(s) 1206
PfeI GAWTC 8 cut(s) 309, 555, 981, 989, 1388, 1649, 2255, 3476
PflMI CCANNNNNTGG 1 cut(s) 2696
PfoI TCCNGGA 2 cut(s) 2689, 3196
Ppu21I YACGTR 1 cut(s) 3061
PpuMI RGGWCCY 4 cut(s) 1353, 1530, 1614, 1791
PscI ACATGT 3 cut(s) 163, 877, 2708
PshBI ATTAAT 1 cut(s) 2772
PsiI TTATAA 2 cut(s) 1307, 1568
Psp1406I AACGTT 1 cut(s) 1863
Psp5II RGGWCCY 4 cut(s) 1353, 1530, 1614, 1791
Psp6I CCWGG 7 cut(s) 900, 2145, 2506, 2610, 2689, 3046, 3196
PspGI CCWGG 7 cut(s) 900, 2145, 2506, 2610, 2689, 3046, 3196
PspN4I GGNNCC 6 cut(s) 52, 66, 1354, 1615, 2108, 2335
PspPPI RGGWCCY 4 cut(s) 1353, 1530, 1614, 1791
PstI CTGCAG 1 cut(s) 3262
PstNI CAGNNNCTG 1 cut(s) 2720
PsuI RGATCY 3 cut(s) 1114, 2521, 3385
PvuII CAGCTG 1 cut(s) 3134
RigI GGCCGGCC 2 cut(s) 396, 642
RsaI GTAC 7 cut(s) 719, 740, 876, 2187, 2345, 3358, 3500
RsaNI GTAC 7 cut(s) 718, 739, 875, 2186, 2344, 3357, 3499
RseI CAYNNNNRTG 6 cut(s) 1147, 1263, 1458, 1505, 1719, 1766
ScaI AGTACT 2 cut(s) 740, 3358
ScrFI CCNGG 9 cut(s) 758, 872, 902, 2147, 2508, 2612, 2691, 3048, 3198
SduI GDGCHC 3 cut(s) 1502, 1763, 3377
SexAI ACCWGGT 1 cut(s) 900
SfcI CTRYAG 2 cut(s) 2965, 3258
SfuI TTCGAA 2 cut(s) 41, 3001
SinI GGWCC 7 cut(s) 64, 1027, 1353, 1530, 1614, 1791, 3536
SmiMI CAYNNNNRTG 6 cut(s) 1147, 1263, 1458, 1505, 1719, 1766
SmlI CTYRAG 7 cut(s) 686, 721, 1832, 1901, 2012, 2058, 2617
SmoI CTYRAG 7 cut(s) 686, 721, 1832, 1901, 2012, 2058, 2617
SphI GCATGC 3 cut(s) 2072, 2122, 2130
SseBI AGGCCT 1 cut(s) 2368
SsiI CCGC 7 cut(s) 220, 466, 995, 2854, 2875, 3043, 3406
SspI AATATT 1 cut(s) 1174
SspMI CTAG 5 cut(s) 1247, 2123, 2189, 2364, 3030
StuI AGGCCT 1 cut(s) 2368
StyD4I CCNGG 9 cut(s) 756, 870, 900, 2145, 2506, 2610, 2689, 3046, 3196
TaaI ACNGT 7 cut(s) 378, 446, 624, 787, 2164, 2359, 2393
TaiI ACGT 3 cut(s) 957, 1866, 3063
TaqI TCGA 5 cut(s) 41, 79, 2784, 3001, 3435
TatI WGTACW 4 cut(s) 717, 738, 2343, 3356
TauI GCSGC 1 cut(s) 3046
TfiI GAWTC 8 cut(s) 309, 555, 981, 989, 1388, 1649, 2255, 3476
TscAI CASTG 3 cut(s) 1507, 1768, 2601
TseFI GTSAC 2 cut(s) 904, 2164
TseI GCWGC 9 cut(s) 145, 335, 581, 853, 1484, 1745, 2638, 2759, 3260
Tsp45I GTSAC 2 cut(s) 904, 2164
TspDTI ATGAA 7 cut(s) 1173, 1230, 2230, 2265, 2280, 3082, 3099
TspGWI ACGGA 2 cut(s) 117, 1800
TspRI CASTG 3 cut(s) 1507, 1768, 2601
Van91I CCANNNNNTGG 1 cut(s) 2696
Vha464I CTTAAG 1 cut(s) 2617
VpaK11BI GGWCC 7 cut(s) 64, 1027, 1353, 1530, 1614, 1791, 3536
VspI ATTAAT 1 cut(s) 2772
XbaI TCTAGA 1 cut(s) 1246
XceI RCATGY 9 cut(s) 167, 881, 912, 1457, 1718, 2072, 2122, 2130, 2712
XmnI GAANNNNTTC 1 cut(s) 1206
XspI CTAG 5 cut(s) 1247, 2123, 2189, 2364, 3030
ZrmI AGTACT 2 cut(s) 740, 3358
Zsp2I ATGCAT 1 cut(s) 2120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.