Rh1BG063000

Retinoblastoma-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0003674
Physical Location & Seq
Reverse (-)
9343452 .. 9351612
8161 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG063000.1

Sequence Viewer

Length: 3072 bp
ATGAGTCCACCTGCTGTGCTGAACATGGAAGATACGAAGCCGGAGGTCTCTGCATCCAACAATTCGAATTCGGGAACTGGAGATTTGGACCCTGTTAAAGCTCGATTTACTGATTTGTGCAAGAATGGATTGTCGTTGGACGAGAACACGCTGGCACAAGCTATCAAGCTGCTGATAGAAACGAAACATGTTCTAATATCAAATTTATCAGCTATCGGAAATGGAACGCCTGAAGAAGCAGAGCGGTTCTGGTTTTCGTTTGTTTTGTTCTCGGTGAAAACATTGAGTGAGAAGAGTTCAGATAATTCGCAAAAGGGGTCTGATGATGGTGGATTCTCTCTCATCCAAATTTTGAGAGCTGCTAAACTGAACATTGTGGATTTCTTTAAGGAGCTTCCACAGTTTATTGTTAAGGCCGGCCCAATTTTAAGTAATCTGTATGGCGTAGATTGGGAGAGCAAACTTGAGGCAAAAGAGTTGCAGGCCAATTTTCTGTACTTGAGCCTCTTAAGCAAGTACTTCAAAAAAGCATACCGGGAATTCTTCTTGACAAGTGATGCAACTGTTGATAAGAAGTCAGCCATTACCAATGGAGCTGGCTATGTGTCAGAGTATCATAGATTTGGATGGCTGCTGTTTTTGGCCCTTCGGGTACATGTATTTGGGCGAGTTAAAGACCTGGTGACATGCACAAACGGCTTGGTTTCCATATTGGCAATTTTAATTATTCACGTCCCCGTTCGCTTCAGAAACTTTAGAATCCATGATTCACCGCGTTTTGTGAGGAAAGGCAACAAAGGTGTGGACCTGCTTGCATCACTTTGCAACACTTATGACACCTCAGAAGAGGAGTTGAGAAAAACAATGGAAAAGGCTAATACATTGATATCAGATCTTTTGAAGAAGAAGCCTTGTTTGGCATCTGAGTGTAAAAATGAAAATCTAAAGAATATTGATACAGATGGTTTGACCTATTTTGAAGGACTTCTGGATGAACCTTCTTTGTCATCCAGTTTAGATATTCTAGAAAAAGATCATGATGATGCAATTCGGAATAAGGGTGAATTGGATGAGAGAGTATTTATAAATGAGGAGGACAGCTTACTTGGTTCAGGGAGCTTGTCAGGAGGGTCCTTGAATTTATCTGGTGTCAAGAGGAAATTTGATTCAATGGCCTCCCCAACAAAGACAATTACAAGTCCACTCTCTCCCCATCGCTCTCCAGCCTCGCATGTTAATGGTGGTGTTAATTCAAAGATGGCAGCTACACCTGTGAGCACTGCAATGACAACTGCAAAGTGGCTTAGGACCTTTATTTCCCCACTTCCATCAAAACCCTCACCCGAGCTCGAGGGTTTTCTGGCATCATGTGATAGGAACGTTAGTAATGATGTGATACGCAGGGCGCAAATAATACTTGAGGCCATATTTCCAAGTAGTGCTTTGGGGGAGCGATGTGTAACTGGTAGTCTGCAAAGTGCTAACCTCATGGACAATGTGTGGGCAGAACAACGAAGACTGGAAGCACTCAAGCTTTATTATAGAGTTTTGGAAGCAATGTGTAGGGCAGAGGCTCAAGTTCTGCATGCTACTAATTTGACCTCTTTATTAACAAATGAGAGGTTCCATAGATGCATGCTAGCATGCTCTGCTGAGTTAGTCCTGGCAACACATAAGACGGTCACAATGTTGTTTCCTGCCGTACTAGAAAGGACAGGCATTACAGCTTTTGATCTTAGCAAGGTGATAGAGAGCTTCGTTAGACATGAAGAATCTCTACCAAGAGAATTGAGACGACATCTAAATTCATTGGAAGAACGACTTTTGGAGAGCATGGTATGGGTGAAGGGTTCCTCAATGTACAATTCTTTGACAGTTGCTAGGCCTGCCTTCTCGGCTGAAATCAACCGTCTTGGGTTATTAGCAGAACCAATGCCATCTTTGGATGCAATAGCTTTGCATATCAACTTCTCTTGTGGAGGGTTGCCTCCAGTAGCTTCTTTACAGAAGCATGAGACTTCCCCAGGTCAGAATAGAGATCTCAGGTCTCCGAAGAGACCCTGCACAGAATATAGGGGTGTATTACTGGAGCGAAATTCCTTTACTTCTCCAGTGAAGGATCGTCTCCTGGCCTTAGGTAACCTTAAATCAAAGCTGCCACCGCCACCTCTACAATCTGCATTTGCAAGTCCAACACGACCAAATCCAGCAGGTGGAGGGGAAACATGTGCAGAAACTGGAGTTAGTATTTTCTTTAATAAGATCATCAAGCTGGCTGCTGTAAGAATTAATGGTATGGTCGAAAGGTTGCAATTGTCTCAGCAAATCAGGGAGAATGTTTATCGCCTCTTCCAACAAATACTTGTTCAGCGGACATCTCTCTTTTTTAACCGCCATATTGACCAAATTATCCTTTGTTGTTTTTACGGGGTTGCCAAGATTTCTCAACTAAGTCTTACATTTAGGGAAATCATACACAACTACAGGAAGCAACCACAATGCAAACCACAAGTTTTTCGAAGTGTCTTTGTTGACTGGTCATCAGCTAGACGCAATGGGCGGCCTGGACAGGAACACGTAGATATCATTAGATTTTACAATGAAGTATTCATTCCTTCTGTCAAGACCCTGCTTGTTGAGCTTGGGCCAGCTGTTACAACTACAAGAGCCAATGTAGTTCCTGAAGCTAACAACAACAATGATGGATCACCTAAAGTATCCACCTTTCCAAGTCTCCCTGATATGTCTCCAAAGAAAGTATCTGCGGCACACAATGTTTATGTATCTCCATTGCGATCATCAAAGATGGATGCCTTAATCTCACATAGCTCAAAGAGCTATTATGCTTGTGTTGGGGAGAGTACTCATGCGTATCAGAGCCCTTCAAAAGATCTGACAGCCATTAACAACCGCTTGAATGGCACACGAAAACGCACACTCGACTTTGATAATGTGGATGTAGGGGTTGGTTTGGTTAGTGATTCTATGGTGGCCAATAGCTTGTACCTTCAAAATGGGAGTTGTGCATCCTCATCGGGCGGACCATTGAAAACCGAGCAACCTGACTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000082 GO:0000278 GO:0000280 GO:0000902 GO:0000904 GO:0001558 GO:0001708 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006275 GO:0006349 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007059 GO:0007127 GO:0007129 GO:0007275 GO:0008134 GO:0008150 GO:0008152 GO:0008356 GO:0009553 GO:0009555 GO:0009566 GO:0009567 GO:0009653 GO:0009791 GO:0009888 GO:0009889 GO:0009987 GO:0010016 GO:0010026 GO:0010052 GO:0010090 GO:0010103 GO:0010374 GO:0010377 GO:0010468 GO:0010556 GO:0010564 GO:0016043 GO:0019219 GO:0019222 GO:0019953 GO:0022402 GO:0022414 GO:0022619 GO:0030154 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032875 GO:0032989 GO:0033043 GO:0034641 GO:0040008 GO:0040029 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0044770 GO:0044772 GO:0044843 GO:0045132 GO:0045143 GO:0045165 GO:0046483 GO:0048229 GO:0048285 GO:0048366 GO:0048367 GO:0048468 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051052 GO:0051128 GO:0051171 GO:0051239 GO:0051276 GO:0051301 GO:0051321 GO:0051704 GO:0051726 GO:0051783 GO:0055046 GO:0060255 GO:0061982 GO:0065007 GO:0070192 GO:0071514 GO:0071704 GO:0071840 GO:0080090 GO:0090329 GO:0090558 GO:0090626 GO:0090627 GO:0090698 GO:0097159 GO:0098813 GO:0099402 GO:0140013 GO:1901360 GO:1901363 GO:1903046 GO:1903047 GO:1903866 GO:2000036 GO:2000112 GO:2000653
KEGG Pathways
Metabolic & Signaling
ko04110 ko04218 ko04350 ko05165 ko05203 map04110 map04218 map04350 map05165 map05203
Pfam Domains
Protein Families
DUF3452 RB_A RB_B Rb_C

Protein Analysis

1023

Amino Acids

113.22

Weight (kDa)

8.81

Isoelectric Point (pI)

49.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3452 PF11934 107 - 244 3.2e-33 Domain of unknown function (DUF3452)
RB_A PF01858 423 - 624 1.3e-63 Retinoblastoma-associated protein A domain
RB_B PF01857 748 - 875 2.2e-43 Retinoblastoma-associated protein B domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1085
AarI CACCTGC 2 cut(s) 19, 2201
Acc36I ACCTGC 3 cut(s) 19, 816, 2201
AccB7I CCANNNNNTGG 1 cut(s) 2213
AccBSI CCGCTC 1 cut(s) 244
AccII CGCG 1 cut(s) 775
AciI CCGC 9 cut(s) 244, 773, 2162, 2371, 2392, 2560, 2765, 2911, 3039
AclI AACGTT 1 cut(s) 1380
AclWI GGATC 2 cut(s) 2127, 2713
AcoI YGGCCR 1 cut(s) 2991
AcsI RAATTY 8 cut(s) 67, 202, 348, 539, 1138, 1160, 1804, 2095
AcuI CTGAAG 3 cut(s) 252, 730, 2703
AfaI GTAC 7 cut(s) 497, 518, 654, 1704, 1862, 2863, 3005
AfiI CCNNNNNNNGG 1 cut(s) 2213
AflII CTTAAG 1 cut(s) 508
AflIII ACRYGT 4 cut(s) 187, 655, 2225, 2575
AjiI CACGTC 1 cut(s) 733
AjnI CCWGG 5 cut(s) 678, 1662, 2023, 2127, 2563
AjuI GAANNNNNNNTTGG 2 cut(s) 899, 931
Alw21I GWGCWC 2 cut(s) 1280, 1350
Alw26I GTCTC 9 cut(s) 52, 1786, 2009, 2050, 2052, 2129, 2322, 2738, 2751
AlwI GGATC 2 cut(s) 2127, 2713
AlwNI CAGNNNCTG 1 cut(s) 2237
Ama87I CYCGRG 2 cut(s) 1343, 1349
ApeKI GCWGC 6 cut(s) 169, 359, 631, 1262, 2155, 2276
ApoI RAATTY 8 cut(s) 67, 202, 348, 539, 1138, 1160, 1804, 2095
AseI ATTAAT 1 cut(s) 2289
Asp700I GAANNNNTTC 1 cut(s) 984
AspLEI GCGC 1 cut(s) 1408
AspS9I GGNCC 8 cut(s) 88, 419, 643, 805, 1131, 1308, 2645, 3041
AsuC2I CCSGG 1 cut(s) 536
AsuHPI GGTGA 8 cut(s) 286, 694, 762, 1073, 1332, 1756, 1855, 2700
AsuII TTCGAA 2 cut(s) 65, 2518
AsuNHI GCTAGC 1 cut(s) 1639
AvaI CYCGRG 2 cut(s) 1343, 1349
AvaII GGWCC 5 cut(s) 88, 805, 1131, 1308, 3041
AxyI CCTNAGG 1 cut(s) 2134
BalI TGGCCA 1 cut(s) 2993
BanII GRGCYC 2 cut(s) 1350, 2882
BarI GAAGNNNNNNTAC 2 cut(s) 1761, 1793
BbsI GAAGAC 1 cut(s) 1522
Bbv12I GWGCWC 2 cut(s) 1280, 1350
BbvI GCAGC 6 cut(s) 156, 346, 618, 1274, 2142, 2263
BccI CCATC 9 cut(s) 320, 621, 956, 1221, 1252, 1336, 1945, 2696, 2800
BceAI ACGGC 2 cut(s) 712, 1685
BcgI CGANNNNNNTGC 2 cut(s) 3015, 3049
BciT130I CCWGG 5 cut(s) 680, 1664, 2025, 2129, 2565
BciVI GTATCC 1 cut(s) 2728
BcnI CCSGG 1 cut(s) 536
BcoDI GTCTC 9 cut(s) 52, 1786, 2009, 2050, 2052, 2129, 2322, 2738, 2751
BfaI CTAG 5 cut(s) 1025, 1640, 1706, 1881, 2547
BfmI CTRYAG 1 cut(s) 2482
BfrI CTTAAG 1 cut(s) 508
BfuAI ACCTGC 3 cut(s) 19, 816, 2201
BfuI GTATCC 1 cut(s) 2728
BglI GCCNNNNNGGC 1 cut(s) 1895
BglII AGATCT 3 cut(s) 892, 2038, 2890
BisI GCNGC 8 cut(s) 170, 360, 632, 1263, 2156, 2277, 2561, 2766
BlsI GCNGC 8 cut(s) 171, 361, 633, 1264, 2157, 2278, 2562, 2767
BmcAI AGTACT 2 cut(s) 518, 2863
Bme1390I CCNGG 6 cut(s) 536, 680, 1664, 2025, 2129, 2565
Bme18I GGWCC 5 cut(s) 88, 805, 1131, 1308, 3041
BmeT110I CYCGRG 2 cut(s) 1343, 1349
BmgBI CACGTC 1 cut(s) 733
BmgT120I GGNCC 8 cut(s) 88, 419, 643, 805, 1131, 1308, 2645, 3041
BmiI GGNNCC 4 cut(s) 90, 1132, 1625, 1852
BmrFI CCNGG 6 cut(s) 536, 680, 1664, 2025, 2129, 2565
BmtI GCTAGC 1 cut(s) 1643
BpiI GAAGAC 1 cut(s) 1522
BpmI CTGGAG 6 cut(s) 99, 1206, 1974, 2094, 2108, 2259
Bpu10I CCTNAGC 1 cut(s) 1304
Bpu14I TTCGAA 2 cut(s) 65, 2518
BpuEI CTTGAG 5 cut(s) 485, 520, 1439, 1514, 1560
BpuMI CCSGG 1 cut(s) 536
BsaAI YACGTR 1 cut(s) 2578
BsaI GGTCTC 3 cut(s) 52, 2050, 2052
BsaJI CCNNGG 1 cut(s) 2023
Bsc4I CCNNNNNNNGG 1 cut(s) 2213
Bse118I RCCGGY 1 cut(s) 416
Bse1I ACTGG 9 cut(s) 82, 1011, 1468, 1524, 1991, 2091, 2111, 2242, 2540
Bse21I CCTNAGG 1 cut(s) 2134
Bse3DI GCAATG 4 cut(s) 1290, 1563, 2560, 2789
BseBI CCWGG 5 cut(s) 680, 1664, 2025, 2129, 2565
BseDI CCNNGG 1 cut(s) 2023
BseLI CCNNNNNNNGG 1 cut(s) 2213
BseMI GCAATG 4 cut(s) 1290, 1563, 2560, 2789
BseMII CTCAG 5 cut(s) 855, 915, 1644, 2056, 2333
BseNI ACTGG 9 cut(s) 82, 1011, 1468, 1524, 1991, 2091, 2111, 2242, 2540
BseRI GAGGAG 2 cut(s) 863, 1106
BseXI GCAGC 6 cut(s) 156, 346, 618, 1274, 2142, 2263
BsgI GTGCAG 2 cut(s) 2047, 2250
Bsh1236I CGCG 1 cut(s) 775
BsiHKAI GWGCWC 2 cut(s) 1280, 1350
BsiHKCI CYCGRG 2 cut(s) 1343, 1349
BsiSI CCGG 3 cut(s) 41, 417, 535
BslFI GGGAC 1 cut(s) 719
BslI CCNNNNNNNGG 1 cut(s) 2213
BsmAI GTCTC 9 cut(s) 52, 1786, 2009, 2050, 2052, 2129, 2322, 2738, 2751
BsmBI CGTCTC 2 cut(s) 1786, 2129
BsmFI GGGAC 1 cut(s) 719
Bso31I GGTCTC 3 cut(s) 52, 2050, 2052
BsoBI CYCGRG 2 cut(s) 1343, 1349
Bsp119I TTCGAA 2 cut(s) 65, 2518
Bsp1286I GDGCHC 3 cut(s) 1280, 1350, 2882
Bsp1407I TGTACA 1 cut(s) 1860
Bsp143I GATC 9 cut(s) 892, 1033, 1732, 2038, 2119, 2262, 2705, 2795, 2890
BspACI CCGC 9 cut(s) 244, 773, 2162, 2371, 2392, 2560, 2765, 2911, 3039
BspCNI CTCAG 5 cut(s) 854, 916, 1645, 2055, 2332
BspFNI CGCG 1 cut(s) 775
BspHI TCATGA 1 cut(s) 1036
BspLI GGNNCC 4 cut(s) 90, 1132, 1625, 1852
BspMI ACCTGC 3 cut(s) 19, 816, 2201
BspOI GCTAGC 1 cut(s) 1643
BspPI GGATC 2 cut(s) 2127, 2713
BspT104I TTCGAA 2 cut(s) 65, 2518
BspTI CTTAAG 1 cut(s) 508
BspTNI GGTCTC 3 cut(s) 52, 2050, 2052
BsrBI CCGCTC 1 cut(s) 244
BsrDI GCAATG 4 cut(s) 1290, 1563, 2560, 2789
BsrFI RCCGGY 1 cut(s) 416
BsrGI TGTACA 1 cut(s) 1860
BsrI ACTGG 9 cut(s) 82, 1011, 1468, 1524, 1991, 2091, 2111, 2242, 2540
BssAI RCCGGY 1 cut(s) 416
BssECI CCNNGG 1 cut(s) 2023
BssMI GATC 9 cut(s) 892, 1033, 1732, 2038, 2119, 2262, 2705, 2795, 2890
Bst2UI CCWGG 5 cut(s) 680, 1664, 2025, 2129, 2565
Bst4CI ACNGT 5 cut(s) 402, 565, 1681, 1876, 1910
Bst6I CTCTTC 4 cut(s) 287, 840, 2048, 2354
BstAFI CTTAAG 1 cut(s) 508
BstAPI GCANNNNNTGC 1 cut(s) 1649
BstAUI TGTACA 1 cut(s) 1860
BstBAI YACGTR 1 cut(s) 2578
BstBI TTCGAA 2 cut(s) 65, 2518
BstDEI CTNAG 9 cut(s) 841, 924, 1304, 1653, 1736, 2042, 2134, 2319, 2450
BstEII GGTNACC 1 cut(s) 2138
BstFNI CGCG 1 cut(s) 775
BstHHI GCGC 1 cut(s) 1408
BstKTI GATC 9 cut(s) 895, 1036, 1735, 2041, 2122, 2265, 2708, 2798, 2893
BstMAI GTCTC 9 cut(s) 52, 1786, 2009, 2050, 2052, 2129, 2322, 2738, 2751
BstMBI GATC 9 cut(s) 892, 1033, 1732, 2038, 2119, 2262, 2705, 2795, 2890
BstMWI GCNNNNNNNGC 9 cut(s) 510, 696, 1649, 1886, 1895, 2161, 2638, 2835, 2919
BstNI CCWGG 5 cut(s) 680, 1664, 2025, 2129, 2565
BstNSI RCATGY 8 cut(s) 191, 659, 690, 1235, 1589, 1639, 1647, 2229
BstPI GGTNACC 1 cut(s) 2138
BstSCI CCNGG 6 cut(s) 534, 678, 1662, 2023, 2127, 2563
BstSFI CTRYAG 1 cut(s) 2482
BstUI CGCG 1 cut(s) 775
BstV1I GCAGC 6 cut(s) 156, 346, 618, 1274, 2142, 2263
BstV2I GAAGAC 1 cut(s) 1522
BstX2I RGATCY 3 cut(s) 892, 2038, 2890
BstYI RGATCY 3 cut(s) 892, 2038, 2890
Bsu36I CCTNAGG 1 cut(s) 2134
BsuI GTATCC 1 cut(s) 2728
BtgZI GCGATG 2 cut(s) 1199, 1468
BtrI CACGTC 1 cut(s) 733
BtsI GCAGTG 1 cut(s) 1278
BtsIMutI CAGTG 2 cut(s) 1278, 2118
BveI ACCTGC 3 cut(s) 19, 816, 2201
CaiI CAGNNNCTG 1 cut(s) 2237
CciI TCATGA 1 cut(s) 1036
CfoI GCGC 1 cut(s) 1408
Cfr10I RCCGGY 1 cut(s) 416
Cfr13I GGNCC 8 cut(s) 88, 419, 643, 805, 1131, 1308, 2645, 3041
CseI GACGC 1 cut(s) 2559
CsiI ACCWGGT 1 cut(s) 678
Csp6I GTAC 7 cut(s) 496, 517, 653, 1703, 1861, 2862, 3004
CviQI GTAC 7 cut(s) 496, 517, 653, 1703, 1861, 2862, 3004
DdeI CTNAG 9 cut(s) 841, 924, 1304, 1653, 1736, 2042, 2134, 2319, 2450
DpnI GATC 9 cut(s) 894, 1035, 1734, 2040, 2121, 2264, 2707, 2797, 2892
DpnII GATC 9 cut(s) 892, 1033, 1732, 2038, 2119, 2262, 2705, 2795, 2890
EaeI YGGCCR 1 cut(s) 2991
Eam1104I CTCTTC 4 cut(s) 287, 840, 2048, 2354
EarI CTCTTC 4 cut(s) 287, 840, 2048, 2354
EciI GGCGGA 1 cut(s) 3054
Ecl136II GAGCTC 1 cut(s) 1348
Eco147I AGGCCT 1 cut(s) 1885
Eco24I GRGCYC 2 cut(s) 1350, 2882
Eco31I GGTCTC 3 cut(s) 52, 2050, 2052
Eco32I GATATC 2 cut(s) 888, 2584
Eco47I GGWCC 5 cut(s) 88, 805, 1131, 1308, 3041
Eco53kI GAGCTC 1 cut(s) 1348
Eco57I CTGAAG 3 cut(s) 252, 730, 2703
Eco81I CCTNAGG 1 cut(s) 2134
Eco88I CYCGRG 2 cut(s) 1343, 1349
Eco91I GGTNACC 1 cut(s) 2138
EcoICRI GAGCTC 1 cut(s) 1348
EcoO109I RGGNCCY 2 cut(s) 1131, 1308
EcoO65I GGTNACC 1 cut(s) 2138
EcoRI GAATTC 2 cut(s) 67, 539
EcoRII CCWGG 5 cut(s) 678, 1662, 2023, 2127, 2563
EcoRV GATATC 2 cut(s) 888, 2584
EcoT22I ATGCAT 1 cut(s) 1637
EcoT38I GRGCYC 2 cut(s) 1350, 2882
Esp3I CGTCTC 2 cut(s) 1786, 2129
FalI AAGNNNNNCTT 4 cut(s) 1426, 1458, 1806, 1838
FaqI GGGAC 1 cut(s) 719
Fnu4HI GCNGC 8 cut(s) 170, 360, 632, 1263, 2156, 2277, 2561, 2766
FriOI GRGCYC 2 cut(s) 1350, 2882
FseI GGCCGGCC 1 cut(s) 420
Fsp4HI GCNGC 8 cut(s) 170, 360, 632, 1263, 2156, 2277, 2561, 2766
FspBI CTAG 5 cut(s) 1025, 1640, 1706, 1881, 2547
GlaI GCGC 1 cut(s) 1407
GluI GCNGC 8 cut(s) 170, 360, 632, 1263, 2156, 2277, 2561, 2766
GsuI CTGGAG 6 cut(s) 99, 1206, 1974, 2094, 2108, 2259
HapII CCGG 3 cut(s) 41, 417, 535
HgaI GACGC 1 cut(s) 2559
HhaI GCGC 1 cut(s) 1408
Hin6I GCGC 1 cut(s) 1406
HinP1I GCGC 1 cut(s) 1406
HincII GTYRAC 1 cut(s) 2533
HindII GTYRAC 1 cut(s) 2533
HindIII AAGCTT 1 cut(s) 1532
HinfI GANTC 7 cut(s) 4, 333, 759, 767, 1166, 1772, 2981
HpaII CCGG 3 cut(s) 41, 417, 535
HphI GGTGA 8 cut(s) 286, 694, 762, 1073, 1332, 1756, 1855, 2700
Hpy166II GTNNAC 4 cut(s) 8, 805, 1202, 2533
Hpy188III TCNNGA 9 cut(s) 72, 547, 989, 1025, 1037, 1125, 1153, 2623, 2681
Hpy8I GTNNAC 4 cut(s) 8, 805, 1202, 2533
HpyAV CCTTC 9 cut(s) 656, 974, 1008, 1840, 1900, 2110, 2625, 2892, 3017
HpyCH4III ACNGT 5 cut(s) 402, 565, 1681, 1876, 1910
HpyCH4IV ACGT 3 cut(s) 732, 1380, 2577
HpyF10VI GCNNNNNNNGC 9 cut(s) 510, 696, 1649, 1886, 1895, 2161, 2638, 2835, 2919
HpyF3I CTNAG 9 cut(s) 841, 924, 1304, 1653, 1736, 2042, 2134, 2319, 2450
HpySE526I ACGT 3 cut(s) 732, 1380, 2577
HspAI GCGC 1 cut(s) 1406
KroI GCCGGC 1 cut(s) 416
KroNI GCCGGC 1 cut(s) 418
Kzo9I GATC 9 cut(s) 892, 1033, 1732, 2038, 2119, 2262, 2705, 2795, 2890
LmnI GCTCC 5 cut(s) 391, 593, 1116, 1450, 2089
Lsp1109I GCAGC 6 cut(s) 156, 346, 618, 1274, 2142, 2263
MabI ACCWGGT 1 cut(s) 678
MaeI CTAG 5 cut(s) 1025, 1640, 1706, 1881, 2547
MaeII ACGT 3 cut(s) 732, 1380, 2577
MaeIII GTNAC 5 cut(s) 682, 1459, 1681, 2138, 2653
MalI GATC 9 cut(s) 894, 1035, 1734, 2040, 2121, 2264, 2707, 2797, 2892
MbiI CCGCTC 1 cut(s) 244
MboI GATC 9 cut(s) 892, 1033, 1732, 2038, 2119, 2262, 2705, 2795, 2890
MfeI CAATTG 1 cut(s) 2312
MflI RGATCY 3 cut(s) 892, 2038, 2890
MhlI GDGCHC 3 cut(s) 1280, 1350, 2882
MlsI TGGCCA 1 cut(s) 2993
MluNI TGGCCA 1 cut(s) 2993
MlyI GAGTC 1 cut(s) 13
MmeI TCCRAC 4 cut(s) 81, 117, 2216, 2377
Mox20I TGGCCA 1 cut(s) 2993
Mph1103I ATGCAT 1 cut(s) 1637
MroNI GCCGGC 1 cut(s) 416
MroXI GAANNNNTTC 1 cut(s) 984
MscI TGGCCA 1 cut(s) 2993
MslI CAYNNNNRTG 4 cut(s) 925, 1041, 1236, 1283
Msp20I TGGCCA 1 cut(s) 2993
MspA1I CMGCKG 2 cut(s) 2371, 2651
MspCI CTTAAG 1 cut(s) 508
MspI CCGG 3 cut(s) 41, 417, 535
MspR9I CCNGG 6 cut(s) 536, 680, 1664, 2025, 2129, 2565
MunI CAATTG 1 cut(s) 2312
MvaI CCWGG 5 cut(s) 680, 1664, 2025, 2129, 2565
MvnI CGCG 1 cut(s) 775
MwoI GCNNNNNNNGC 9 cut(s) 510, 696, 1649, 1886, 1895, 2161, 2638, 2835, 2919
NaeI GCCGGC 1 cut(s) 418
NciI CCSGG 1 cut(s) 536
NdeII GATC 9 cut(s) 892, 1033, 1732, 2038, 2119, 2262, 2705, 2795, 2890
NgoMIV GCCGGC 1 cut(s) 416
NheI GCTAGC 1 cut(s) 1639
NlaIV GGNNCC 4 cut(s) 90, 1132, 1625, 1852
NmeAIII GCCGAG 1 cut(s) 1874
NmuCI GTSAC 2 cut(s) 682, 1681
NsiI ATGCAT 1 cut(s) 1637
NspI RCATGY 8 cut(s) 191, 659, 690, 1235, 1589, 1639, 1647, 2229
NspV TTCGAA 2 cut(s) 65, 2518
PaeI GCATGC 3 cut(s) 1589, 1639, 1647
PaeR7I CTCGAG 1 cut(s) 1349
PagI TCATGA 1 cut(s) 1036
PaqCI CACCTGC 2 cut(s) 19, 2201
PceI AGGCCT 1 cut(s) 1885
PciI ACATGT 3 cut(s) 187, 655, 2225
PdiI GCCGGC 1 cut(s) 418
PdmI GAANNNNTTC 1 cut(s) 984
PfeI GAWTC 6 cut(s) 333, 759, 767, 1166, 1772, 2981
PflMI CCANNNNNTGG 1 cut(s) 2213
PkrI GCNGC 8 cut(s) 171, 361, 633, 1264, 2157, 2278, 2562, 2767
PleI GAGTC 1 cut(s) 12
PpsI GAGTC 1 cut(s) 12
Ppu21I YACGTR 1 cut(s) 2578
PpuMI RGGWCCY 2 cut(s) 1131, 1308
PscI ACATGT 3 cut(s) 187, 655, 2225
PshBI ATTAAT 1 cut(s) 2289
PsiI TTATAA 1 cut(s) 1085
Psp124BI GAGCTC 1 cut(s) 1350
Psp1406I AACGTT 1 cut(s) 1380
Psp5II RGGWCCY 2 cut(s) 1131, 1308
Psp6I CCWGG 5 cut(s) 678, 1662, 2023, 2127, 2563
PspEI GGTNACC 1 cut(s) 2138
PspGI CCWGG 5 cut(s) 678, 1662, 2023, 2127, 2563
PspN4I GGNNCC 4 cut(s) 90, 1132, 1625, 1852
PspPI GGNCC 8 cut(s) 88, 419, 643, 805, 1131, 1308, 2645, 3041
PspPPI RGGWCCY 2 cut(s) 1131, 1308
PspXI VCTCGAGB 1 cut(s) 1349
PstNI CAGNNNCTG 1 cut(s) 2237
PsuI RGATCY 3 cut(s) 892, 2038, 2890
PvuII CAGCTG 1 cut(s) 2651
RigI GGCCGGCC 1 cut(s) 420
RsaI GTAC 7 cut(s) 497, 518, 654, 1704, 1862, 2863, 3005
RsaNI GTAC 7 cut(s) 496, 517, 653, 1703, 1861, 2862, 3004
RseI CAYNNNNRTG 4 cut(s) 925, 1041, 1236, 1283
SacI GAGCTC 1 cut(s) 1350
SatI GCNGC 8 cut(s) 170, 360, 632, 1263, 2156, 2277, 2561, 2766
Sau3AI GATC 9 cut(s) 892, 1033, 1732, 2038, 2119, 2262, 2705, 2795, 2890
Sau96I GGNCC 8 cut(s) 88, 419, 643, 805, 1131, 1308, 2645, 3041
ScaI AGTACT 2 cut(s) 518, 2863
SchI GAGTC 1 cut(s) 13
ScrFI CCNGG 6 cut(s) 536, 680, 1664, 2025, 2129, 2565
SduI GDGCHC 3 cut(s) 1280, 1350, 2882
SexAI ACCWGGT 1 cut(s) 678
SfcI CTRYAG 1 cut(s) 2482
Sfr274I CTCGAG 1 cut(s) 1349
SfuI TTCGAA 2 cut(s) 65, 2518
SinI GGWCC 5 cut(s) 88, 805, 1131, 1308, 3041
SlaI CTCGAG 1 cut(s) 1349
SmiMI CAYNNNNRTG 4 cut(s) 925, 1041, 1236, 1283
SmlI CTYRAG 7 cut(s) 464, 499, 508, 1349, 1418, 1529, 1575
SmoI CTYRAG 7 cut(s) 464, 499, 508, 1349, 1418, 1529, 1575
SphI GCATGC 3 cut(s) 1589, 1639, 1647
SseBI AGGCCT 1 cut(s) 1885
SsiI CCGC 9 cut(s) 244, 773, 2162, 2371, 2392, 2560, 2765, 2911, 3039
SspI AATATT 1 cut(s) 952
SspMI CTAG 5 cut(s) 1025, 1640, 1706, 1881, 2547
SstI GAGCTC 1 cut(s) 1350
StuI AGGCCT 1 cut(s) 1885
StyD4I CCNGG 6 cut(s) 534, 678, 1662, 2023, 2127, 2563
TaaI ACNGT 5 cut(s) 402, 565, 1681, 1876, 1910
TaiI ACGT 3 cut(s) 735, 1383, 2580
TaqI TCGA 6 cut(s) 65, 103, 1350, 2301, 2518, 2940
TatI WGTACW 4 cut(s) 495, 516, 1860, 2861
TauI GCSGC 2 cut(s) 2563, 2768
TfiI GAWTC 6 cut(s) 333, 759, 767, 1166, 1772, 2981
TscAI CASTG 2 cut(s) 1285, 2118
TseFI GTSAC 2 cut(s) 682, 1681
TseI GCWGC 6 cut(s) 169, 359, 631, 1262, 2155, 2276
Tsp45I GTSAC 2 cut(s) 682, 1681
TspDTI ATGAA 6 cut(s) 951, 1008, 1782, 1797, 2599, 2616
TspRI CASTG 2 cut(s) 1285, 2118
Van91I CCANNNNNTGG 1 cut(s) 2213
Vha464I CTTAAG 1 cut(s) 508
VpaK11BI GGWCC 5 cut(s) 88, 805, 1131, 1308, 3041
VspI ATTAAT 1 cut(s) 2289
XapI RAATTY 8 cut(s) 67, 202, 348, 539, 1138, 1160, 1804, 2095
XbaI TCTAGA 1 cut(s) 1024
XceI RCATGY 8 cut(s) 191, 659, 690, 1235, 1589, 1639, 1647, 2229
XhoI CTCGAG 1 cut(s) 1349
XmnI GAANNNNTTC 1 cut(s) 984
XspI CTAG 5 cut(s) 1025, 1640, 1706, 1881, 2547
ZrmI AGTACT 2 cut(s) 518, 2863
Zsp2I ATGCAT 1 cut(s) 1637
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.