MD02G1277700.v1.1

Retinoblastoma-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0003674
Physical Location & Seq
Forward (+)
33354199 .. 33362186
7988 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1277700.v1.1.491

Sequence Viewer

Length: 2967 bp
ATGGTGAAGATGGAAGATACTAAGCCTGAAGTTTCACCTTCCATCAATTCAAATTCCGAGAGCAGCGATTCAGGCCCTGTCCAAGATCGATTTACTGATTTGTGCAAGAATGTATTGTCGTTGGACGAGAACTCCTACACACAAGCTGCGAAACTGTTCAAGGAAACCAAACATCTGTTAATATCAAATGCGTCAGCTATTGGCAACGGAACACCTGAAGAAGCAGAACGGTTCTGGTTTGCCTTTGTTTTGTACTCTGTGAAAACATTGAGCAACAAGAATTCGGATAATTCTCAGAAGAGCTCCGATGATAATGGATTTTCTCTCTTCCAAATTTTGAGAGCTACGAAGCTGAACATTGTAGATTTTTTCAAGGAGCTTCCTCAGTTTGTTATTAAGGCTGGTTCAATTTTGAGTAATATTTATGGCGTAGATTGGGAAAACAAACTTGAGGCAAAAGAGCTGCAGGCAAATTTTGTGTACTTGAGCCTGTTAAGCAAGTACTACAAACGTGCGTATCGGGAGTTATTCTTGACAAGTGATACAAATGCCAATAAGCAGTCAGCTGTTGCCAGTGGAACTGGTTATGTGTCAGAGTATCATTGTTTTGGATGGTTGCTGTTTTTGGCCCTTCGTGTACACGTATTCAGCCGATTTAAAGACCTGGTGACTTGCACAAACGGCTTGGTTGCAATTTTGGGAATTTTAATAATTCACGTCCCTGTTCGCTTCAGAAAGTTCAGCATTCATGATTCAACACGCTTTGCAAGGAAAGGCAACAAAGGTGTGGACCTGCTTGCATGGCTTTGCAACACTTATGACACCTCAGACGATGAGTTGAGAAAAACAATGGAAATGGCTAATGGTTTAATAGCAGATATTTTGAAGAAGAAGCCCGTTTCGGCGTCTGATTGTAAAACTGAAAATCTAGAGAATATGGATCCAGATGGTTTGACGTACTTTGAAGGTCTCATGGAGGAACCATCTTTGTCTTCCAGTTTAGATATTCTAGAAAAAAATTACGATGATGCAATTCGGAATAAGGGTGAAGTTGATGAGAGAGTCTTCATCAACGAGGACGATAGCTTACTTGGTTCAGGGAGCTTTTCAGGAGGATCCATGAGTGTAGCTGGTGTGAAGAGGAAATTTGATTCCATAGCTTCCCCAACAAAGACAATTACAAGTCCACTCTCTCCCCATCGCTCTCCTGCATCTCATGTCAATGGTGGTGCTAATTCAAAGATGGTGGCTACACCTGTGAGTACTGCAATGACAACTGCAAAGTGGCTTCGGACCTTTATTTCTCCGCTTCCGTCAAAACCCTCAGCTGAGCTCGAGGGGTTCCTGGCATCGTGTGATAGGGATGTTACTACTGATGTGATACGCAGGGCACAAATTATATTGGAGGCCATATTTCTAGATAGTGCCCTGGGAGAGCGATGTGTAACTGGTAGCCTGCAAGGTGCAAACCTCATGGACAACATCTGGCAAGAGCAACGAAGATTGGAAGCACTCAAACTTTATTATAGAGTTTTGGAAGCAATGTGTAGGGCAGAGGCTCAAGTACTGCATGCAACTAATTTGACCTCCTTATTAACCAATGAGAGGTTTCACAGATGCATGTTAGCCTGCTCTGCAGAGTTAGTCCTGGCAACACATAAGACTGTCACAATGTTGTTTCCAGCAGTATTAGAGAGGACCGGTATTACCGCTTTTGATCTTAGCAAGGTGATAGAGAGTTTCATTAGACATGAAGAATCTCTCCCAAGAGAATTAAGAAGACATCTGAATTCATTGGAAGAACGACTTTTGGAGAGCATGGTATGGGAGAAGGGTTCCTCGATGTATAATTCTTTAACTGTTGCAAGACCTGCCCTCTCAGCAGAAATAAATCGTCTCGGGTTATTAGCAGAGCCCATGCCATCTTTGGATGCAATTGCCATGAATATTAACTTCCCTTGTGGAGGGTTGCCTCCAGTACCTTCTTTACAGAAGCATGAGACTTCACTAGGTCAGAATGGGGATATCAGGTCTCCAAAGAGATCCTGCACAGACTATAGGAGTATATTGCTGGAGCGGAATTCCTTTACATCTCCTGTGAAGGATCGTCTTCTGGCCTTAAGTAACCTTAAGTCAAAGCTGCCACCACCTCCTTTACAATCTGCATTTGCCAGTCCAACACGACCAAATCCAGGGGGTGGAGGGGAAACATGTGCAGAAACTGGAATCAGTATTTTCTTTAGCAAGATAAACAAGCTGGCGGCAGTCAGAATCAATGGAATGGTTGAAAGAATGCAACTGTCTCAGATAAGAGAGAATGTTTATCGTCTCTTTCAACAAATACTTGTTCAGCGGACATCTCTCTTTTTCAACCGCCATATTGACCAGATTATCCTTTGTTGTTTTTATGGAGTTTCAAAGATTTCTCAACTAAATCTCACATTTCGGGAAATCATATACAACTACAGAAAGCAACCACAATGTAAACCGGAAGTTTTTCGAGGCGTGTTTGTTGAGTGGCCTTCACCACGCCGTAATGGGAGACCCGCACAGGAACACGTGGATATCATAACATTTTACAATGAAATATTCATACCTTCTGTGAAGACCCTGCTAGTTGAGCTCGGACCTGCTGTTACAGCAACTACAAGAGCTAACGTGGTTCCTGAAGTTAACAACAATAATGATGCTCCATGCCCTGGATCACCTAAAGTATCTACTTTTCCAAGTCTTCCTGATATGTCTCCAAAGAAAGTTTCTGCAGCGCACAATGTTTACGTCTCTCCATTGCGATCATCTAAGATGGATGCTTTGATATCACATAGCTCAAAAAGCTATTATGCTTGTGTTGGGGAGAGTACTCATGCTTATCAGAGCCCTTCGAAAGATCTCACTGCCATCAACAACCGCTTGAACGGGTATGCTTTTAGTTTATGGATGAGATTAACCAAGGTAAATTGTCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000082 GO:0000278 GO:0000280 GO:0000902 GO:0000904 GO:0001558 GO:0001708 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006275 GO:0006349 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007059 GO:0007127 GO:0007129 GO:0007275 GO:0008134 GO:0008150 GO:0008152 GO:0008356 GO:0009553 GO:0009555 GO:0009566 GO:0009567 GO:0009653 GO:0009791 GO:0009888 GO:0009889 GO:0009987 GO:0010016 GO:0010026 GO:0010052 GO:0010090 GO:0010103 GO:0010374 GO:0010377 GO:0010468 GO:0010556 GO:0010564 GO:0016043 GO:0019219 GO:0019222 GO:0019953 GO:0022402 GO:0022414 GO:0022619 GO:0030154 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032875 GO:0032989 GO:0033043 GO:0034641 GO:0040008 GO:0040029 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0044770 GO:0044772 GO:0044843 GO:0045132 GO:0045143 GO:0045165 GO:0046483 GO:0048229 GO:0048285 GO:0048366 GO:0048367 GO:0048468 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051052 GO:0051128 GO:0051171 GO:0051239 GO:0051276 GO:0051301 GO:0051321 GO:0051704 GO:0051726 GO:0051783 GO:0055046 GO:0060255 GO:0061982 GO:0065007 GO:0070192 GO:0071514 GO:0071704 GO:0071840 GO:0080090 GO:0090329 GO:0090558 GO:0090626 GO:0090627 GO:0090698 GO:0097159 GO:0098813 GO:0099402 GO:0140013 GO:1901360 GO:1901363 GO:1903046 GO:1903047 GO:1903866 GO:2000036 GO:2000112 GO:2000653
KEGG Pathways
Metabolic & Signaling
ko04110 ko04218 ko04350 ko05165 ko05203 map04110 map04218 map04350 map05165 map05203
Pfam Domains
Protein Families
DUF3452 RB_A RB_B Rb_C

Protein Analysis

989

Amino Acids

110.16

Weight (kDa)

8.57

Isoelectric Point (pI)

50.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3452 PF11934 100 - 239 2.2e-35 Domain of unknown function (DUF3452)
RB_A PF01858 418 - 619 2.4e-64 Retinoblastoma-associated protein A domain
RB_B PF01857 743 - 869 1.1e-39 Retinoblastoma-associated protein B domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 801, 1879, 2638
AccB7I CCANNNNNTGG 2 cut(s) 2198, 2699
AccBSI CCGCTC 1 cut(s) 2077
AciI CCGC 8 cut(s) 1307, 1710, 2077, 2261, 2353, 2374, 2547, 2908
AclWI GGATC 7 cut(s) 935, 948, 1110, 1123, 2037, 2112, 2710
AcsI RAATTY 8 cut(s) 52, 280, 333, 472, 702, 1145, 1789, 2080
AcuI CTGAAG 4 cut(s) 48, 237, 715, 2688
AcvI CACGTG 1 cut(s) 2560
AcyI GRCGYC 1 cut(s) 905
AfaI GTAC 9 cut(s) 254, 482, 503, 639, 959, 1264, 1566, 1980, 2860
AfiI CCNNNNNNNGG 5 cut(s) 1605, 1964, 2192, 2198, 2699
AflII CTTAAG 2 cut(s) 2119, 2129
AflIII ACRYGT 3 cut(s) 640, 2210, 2557
AgeI ACCGGT 1 cut(s) 1700
AjiI CACGTC 1 cut(s) 718
AjnI CCWGG 6 cut(s) 663, 1344, 1428, 1647, 2191, 2698
AjuI GAANNNNNNNTTGG 2 cut(s) 2740, 2772
Alw21I GWGCWC 3 cut(s) 305, 1335, 2625
Alw26I GTCTC 9 cut(s) 974, 1901, 1994, 2037, 2307, 2333, 2536, 2748, 2785
AlwI GGATC 7 cut(s) 935, 948, 1110, 1123, 2037, 2112, 2710
AlwNI CAGNNNCTG 2 cut(s) 77, 2222
Ama87I CYCGRG 2 cut(s) 1334, 1898
AoxI GGCC 5 cut(s) 73, 627, 1407, 2115, 2519
ApeKI GCWGC 5 cut(s) 63, 146, 463, 2140, 2762
ApoI RAATTY 8 cut(s) 52, 280, 333, 472, 702, 1145, 1789, 2080
AsiGI ACCGGT 1 cut(s) 1700
Asp700I GAANNNNTTC 2 cut(s) 155, 2496
AspLEI GCGC 1 cut(s) 2767
AspS9I GGNCC 6 cut(s) 74, 628, 790, 1293, 1698, 2627
AsuHPI GGTGA 7 cut(s) 16, 27, 679, 1058, 1741, 2517, 2697
AsuII TTCGAA 1 cut(s) 2882
AvaI CYCGRG 2 cut(s) 1334, 1898
AvaII GGWCC 4 cut(s) 790, 1293, 1698, 2627
BaeGI GKGCMC 2 cut(s) 1393, 1429
BaeI ACNNNNGTAYC 2 cut(s) 500, 533
BamHI GGATCC 2 cut(s) 940, 1115
BanII GRGCYC 5 cut(s) 305, 1335, 1917, 2625, 2879
BbrPI CACGTG 1 cut(s) 2560
BbsI GAAGAC 6 cut(s) 984, 1057, 1786, 2102, 2612, 2723
Bbv12I GWGCWC 3 cut(s) 305, 1335, 2625
BbvCI CCTCAGC 1 cut(s) 1324
BbvI GCAGC 5 cut(s) 75, 133, 450, 2127, 2774
BceAI ACGGC 2 cut(s) 697, 2517
BciT130I CCWGG 6 cut(s) 665, 1346, 1430, 1649, 2193, 2700
BcoDI GTCTC 9 cut(s) 974, 1901, 1994, 2037, 2307, 2333, 2536, 2748, 2785
BfaI CTAG 5 cut(s) 929, 1010, 1418, 2009, 2615
BfmI CTRYAG 5 cut(s) 464, 1635, 2056, 2464, 2760
BfrI CTTAAG 2 cut(s) 2119, 2129
BfuAI ACCTGC 3 cut(s) 801, 1879, 2638
BglII AGATCT 1 cut(s) 2887
BisI GCNGC 6 cut(s) 64, 147, 464, 2141, 2262, 2763
BlpI GCTNAGC 1 cut(s) 1329
BlsI GCNGC 6 cut(s) 65, 148, 465, 2142, 2263, 2764
BmcAI AGTACT 4 cut(s) 503, 1264, 1566, 2860
Bme1390I CCNGG 6 cut(s) 665, 1346, 1430, 1649, 2193, 2700
Bme18I GGWCC 4 cut(s) 790, 1293, 1698, 2627
BmeT110I CYCGRG 2 cut(s) 1334, 1898
BmgBI CACGTC 1 cut(s) 718
BmgT120I GGNCC 6 cut(s) 74, 628, 790, 1293, 1698, 2627
BmiI GGNNCC 6 cut(s) 942, 981, 1117, 1343, 1837, 2664
BmrFI CCNGG 6 cut(s) 665, 1346, 1430, 1649, 2193, 2700
BmsI GCATC 7 cut(s) 1018, 1220, 1358, 1607, 1921, 2677, 2797
BpiI GAAGAC 6 cut(s) 984, 1057, 1786, 2102, 2612, 2723
BpmI CTGGAG 2 cut(s) 1959, 2093
Bpu10I CCTNAGC 1 cut(s) 1324
Bpu1102I GCTNAGC 1 cut(s) 1329
Bpu14I TTCGAA 1 cut(s) 2882
BpuEI CTTGAG 3 cut(s) 470, 505, 1545
Bsa29I ATCGAT 1 cut(s) 88
BsaAI YACGTR 2 cut(s) 643, 2560
BsaHI GRCGYC 1 cut(s) 905
BsaI GGTCTC 3 cut(s) 974, 2037, 2536
BsaJI CCNNGG 5 cut(s) 1428, 1429, 2192, 2698, 2949
BsaWI WCCGGW 2 cut(s) 1700, 2488
BsaXI ACNNNNNCTCC 2 cut(s) 116, 146
Bsc4I CCNNNNNNNGG 5 cut(s) 1605, 1964, 2192, 2198, 2699
Bse118I RCCGGY 1 cut(s) 1700
Bse1I ACTGG 7 cut(s) 573, 586, 996, 1453, 1976, 2172, 2227
Bse3DI GCAATG 3 cut(s) 1275, 1548, 2786
BseBI CCWGG 6 cut(s) 665, 1346, 1430, 1649, 2193, 2700
BseCI ATCGAT 1 cut(s) 88
BseDI CCNNGG 5 cut(s) 1428, 1429, 2192, 2698, 2949
BseGI GGATG 5 cut(s) 617, 1369, 1936, 2812, 2943
BseLI CCNNNNNNNGG 5 cut(s) 1605, 1964, 2192, 2198, 2699
BseMI GCAATG 3 cut(s) 1275, 1548, 2786
BseMII CTCAG 7 cut(s) 308, 398, 840, 1320, 1338, 1893, 2318
BseNI ACTGG 7 cut(s) 573, 586, 996, 1453, 1976, 2172, 2227
BseSI GKGCMC 2 cut(s) 1393, 1429
BseXI GCAGC 5 cut(s) 75, 133, 450, 2127, 2774
BsgI GTGCAG 2 cut(s) 2032, 2235
BshFI GGCC 5 cut(s) 75, 629, 1409, 2117, 2521
BshTI ACCGGT 1 cut(s) 1700
BshVI ATCGAT 1 cut(s) 88
BsiHKAI GWGCWC 3 cut(s) 305, 1335, 2625
BsiHKCI CYCGRG 2 cut(s) 1334, 1898
BsiSI CCGG 2 cut(s) 1701, 2489
BslFI GGGAC 1 cut(s) 704
BslI CCNNNNNNNGG 5 cut(s) 1605, 1964, 2192, 2198, 2699
BsmAI GTCTC 9 cut(s) 974, 1901, 1994, 2037, 2307, 2333, 2536, 2748, 2785
BsmBI CGTCTC 3 cut(s) 1901, 2333, 2785
BsmFI GGGAC 1 cut(s) 704
BsmI GAATGC 2 cut(s) 744, 2298
BsnI GGCC 5 cut(s) 75, 629, 1409, 2117, 2521
Bso31I GGTCTC 3 cut(s) 974, 2037, 2536
BsoBI CYCGRG 2 cut(s) 1334, 1898
Bsp119I TTCGAA 1 cut(s) 2882
Bsp1286I GDGCHC 7 cut(s) 305, 1335, 1393, 1429, 1917, 2625, 2879
Bsp1407I TGTACA 1 cut(s) 637
Bsp143I GATC 9 cut(s) 85, 940, 1115, 1717, 2042, 2104, 2702, 2792, 2887
Bsp1720I GCTNAGC 1 cut(s) 1329
BspACI CCGC 8 cut(s) 1307, 1710, 2077, 2261, 2353, 2374, 2547, 2908
BspANI GGCC 5 cut(s) 75, 629, 1409, 2117, 2521
BspCNI CTCAG 7 cut(s) 307, 397, 839, 1321, 1337, 1892, 2317
BspDI ATCGAT 1 cut(s) 88
BspHI TCATGA 1 cut(s) 748
BspLI GGNNCC 6 cut(s) 942, 981, 1117, 1343, 1837, 2664
BspMAI CTGCAG 3 cut(s) 468, 1639, 2764
BspMI ACCTGC 3 cut(s) 801, 1879, 2638
BspPI GGATC 7 cut(s) 935, 948, 1110, 1123, 2037, 2112, 2710
BspQI GCTCTTC 1 cut(s) 293
BspT104I TTCGAA 1 cut(s) 2882
BspTI CTTAAG 2 cut(s) 2119, 2129
BspTNI GGTCTC 3 cut(s) 974, 2037, 2536
BsrBI CCGCTC 1 cut(s) 2077
BsrDI GCAATG 3 cut(s) 1275, 1548, 2786
BsrFI RCCGGY 1 cut(s) 1700
BsrGI TGTACA 1 cut(s) 637
BsrI ACTGG 7 cut(s) 573, 586, 996, 1453, 1976, 2172, 2227
BssAI RCCGGY 1 cut(s) 1700
BssECI CCNNGG 5 cut(s) 1428, 1429, 2192, 2698, 2949
BssMI GATC 9 cut(s) 85, 940, 1115, 1717, 2042, 2104, 2702, 2792, 2887
BssNI GRCGYC 1 cut(s) 905
BssT1I CCWWGG 1 cut(s) 2949
Bst2UI CCWGG 6 cut(s) 665, 1346, 1430, 1649, 2193, 2700
Bst4CI ACNGT 5 cut(s) 156, 231, 1666, 1861, 2301
Bst6I CTCTTC 3 cut(s) 293, 332, 1133
BstACI GRCGYC 1 cut(s) 905
BstAFI CTTAAG 2 cut(s) 2119, 2129
BstAPI GCANNNNNTGC 1 cut(s) 1871
BstAUI TGTACA 1 cut(s) 637
BstBAI YACGTR 2 cut(s) 643, 2560
BstBI TTCGAA 1 cut(s) 2882
BstC8I GCNNGC 6 cut(s) 468, 798, 1457, 1572, 1630, 2259
BstENI CCTNNNNNAGG 1 cut(s) 1962
BstF5I GGATG 5 cut(s) 617, 1369, 1936, 2812, 2943
BstHHI GCGC 1 cut(s) 2767
BstKTI GATC 9 cut(s) 88, 943, 1118, 1720, 2045, 2107, 2705, 2795, 2890
BstMAI GTCTC 9 cut(s) 974, 1901, 1994, 2037, 2307, 2333, 2536, 2748, 2785
BstMBI GATC 9 cut(s) 85, 940, 1115, 1717, 2042, 2104, 2702, 2792, 2887
BstNI CCWGG 6 cut(s) 665, 1346, 1430, 1649, 2193, 2700
BstNSI RCATGY 3 cut(s) 1574, 1624, 2214
BstSCI CCNGG 6 cut(s) 663, 1344, 1428, 1647, 2191, 2698
BstSFI CTRYAG 5 cut(s) 464, 1635, 2056, 2464, 2760
BstSLI GKGCMC 2 cut(s) 1393, 1429
BstV1I GCAGC 5 cut(s) 75, 133, 450, 2127, 2774
BstV2I GAAGAC 6 cut(s) 984, 1057, 1786, 2102, 2612, 2723
BstX2I RGATCY 4 cut(s) 940, 1115, 2042, 2887
BstYI RGATCY 4 cut(s) 940, 1115, 2042, 2887
Bsu15I ATCGAT 1 cut(s) 88
BsuRI GGCC 5 cut(s) 75, 629, 1409, 2117, 2521
BsuTUI ATCGAT 1 cut(s) 88
BtgZI GCGATG 2 cut(s) 1184, 1453
BtrI CACGTC 1 cut(s) 718
BtsCI GGATG 5 cut(s) 617, 1369, 1936, 2812, 2943
BtsI GCAGTG 1 cut(s) 2892
BtsIMutI CAGTG 2 cut(s) 580, 2892
BveI ACCTGC 3 cut(s) 801, 1879, 2638
Cac8I GCNNGC 6 cut(s) 468, 798, 1457, 1572, 1630, 2259
CaiI CAGNNNCTG 2 cut(s) 77, 2222
CciI TCATGA 1 cut(s) 748
CfoI GCGC 1 cut(s) 2767
Cfr10I RCCGGY 1 cut(s) 1700
Cfr13I GGNCC 6 cut(s) 74, 628, 790, 1293, 1698, 2627
ClaI ATCGAT 1 cut(s) 88
CseI GACGC 2 cut(s) 180, 894
CsiI ACCWGGT 1 cut(s) 663
Csp6I GTAC 9 cut(s) 253, 481, 502, 638, 958, 1263, 1565, 1979, 2859
CspAI ACCGGT 1 cut(s) 1700
CspCI CAANNNNNGTGG 2 cut(s) 1227, 1262
CviQI GTAC 9 cut(s) 253, 481, 502, 638, 958, 1263, 1565, 1979, 2859
DpnI GATC 9 cut(s) 87, 942, 1117, 1719, 2044, 2106, 2704, 2794, 2889
DpnII GATC 9 cut(s) 85, 940, 1115, 1717, 2042, 2104, 2702, 2792, 2887
DraI TTTAAA 1 cut(s) 658
Eam1104I CTCTTC 3 cut(s) 293, 332, 1133
EarI CTCTTC 3 cut(s) 293, 332, 1133
Ecl136II GAGCTC 3 cut(s) 303, 1333, 2623
Eco130I CCWWGG 1 cut(s) 2949
Eco24I GRGCYC 5 cut(s) 305, 1335, 1917, 2625, 2879
Eco31I GGTCTC 3 cut(s) 974, 2037, 2536
Eco32I GATATC 3 cut(s) 2026, 2566, 2817
Eco47I GGWCC 4 cut(s) 790, 1293, 1698, 2627
Eco53kI GAGCTC 3 cut(s) 303, 1333, 2623
Eco57I CTGAAG 4 cut(s) 48, 237, 715, 2688
Eco72I CACGTG 1 cut(s) 2560
Eco88I CYCGRG 2 cut(s) 1334, 1898
EcoICRI GAGCTC 3 cut(s) 303, 1333, 2623
EcoNI CCTNNNNNAGG 1 cut(s) 1962
EcoO109I RGGNCCY 1 cut(s) 74
EcoRI GAATTC 3 cut(s) 280, 1789, 2080
EcoRII CCWGG 6 cut(s) 663, 1344, 1428, 1647, 2191, 2698
EcoRV GATATC 3 cut(s) 2026, 2566, 2817
EcoT14I CCWWGG 1 cut(s) 2949
EcoT22I ATGCAT 1 cut(s) 1622
EcoT38I GRGCYC 5 cut(s) 305, 1335, 1917, 2625, 2879
ErhI CCWWGG 1 cut(s) 2949
Esp3I CGTCTC 3 cut(s) 1901, 2333, 2785
FalI AAGNNNNNCTT 2 cut(s) 1791, 1823
FaqI GGGAC 1 cut(s) 704
FauI CCCGC 1 cut(s) 2554
Fnu4HI GCNGC 6 cut(s) 64, 147, 464, 2141, 2262, 2763
FokI GGATG 5 cut(s) 624, 1376, 1943, 2819, 2950
FriOI GRGCYC 5 cut(s) 305, 1335, 1917, 2625, 2879
Fsp4HI GCNGC 6 cut(s) 64, 147, 464, 2141, 2262, 2763
FspBI CTAG 5 cut(s) 929, 1010, 1418, 2009, 2615
GlaI GCGC 1 cut(s) 2766
GluI GCNGC 6 cut(s) 64, 147, 464, 2141, 2262, 2763
GsuI CTGGAG 2 cut(s) 1959, 2093
HaeIII GGCC 5 cut(s) 75, 629, 1409, 2117, 2521
HapII CCGG 2 cut(s) 1701, 2489
HgaI GACGC 2 cut(s) 180, 894
HhaI GCGC 1 cut(s) 2767
Hin1I GRCGYC 1 cut(s) 905
Hin6I GCGC 1 cut(s) 2765
HinP1I GCGC 1 cut(s) 2765
HincII GTYRAC 1 cut(s) 2674
HindII GTYRAC 1 cut(s) 2674
HinfI GANTC 7 cut(s) 68, 752, 1062, 1151, 1757, 2226, 2271
HpaI GTTAAC 1 cut(s) 2674
HpaII CCGG 2 cut(s) 1701, 2489
HphI GGTGA 7 cut(s) 16, 27, 679, 1058, 1741, 2517, 2697
Hpy166II GTNNAC 8 cut(s) 481, 638, 640, 790, 1187, 2486, 2674, 2776
Hpy8I GTNNAC 8 cut(s) 481, 638, 640, 790, 1187, 2486, 2674, 2776
HpyAV CCTTC 9 cut(s) 48, 641, 959, 1825, 1992, 2095, 2532, 2607, 2889
HpyCH4III ACNGT 5 cut(s) 156, 231, 1666, 1861, 2301
HpyCH4IV ACGT 7 cut(s) 511, 642, 717, 956, 2559, 2658, 2778
HpySE526I ACGT 7 cut(s) 511, 642, 717, 956, 2559, 2658, 2778
Hsp92I GRCGYC 1 cut(s) 905
HspAI GCGC 1 cut(s) 2765
KspAI GTTAAC 1 cut(s) 2674
Kzo9I GATC 9 cut(s) 85, 940, 1115, 1717, 2042, 2104, 2702, 2792, 2887
LguI GCTCTTC 1 cut(s) 293
LmnI GCTCC 5 cut(s) 308, 376, 1101, 2074, 2695
Lsp1109I GCAGC 5 cut(s) 75, 133, 450, 2127, 2774
LweI GCATC 7 cut(s) 1018, 1220, 1358, 1607, 1921, 2677, 2797
MabI ACCWGGT 1 cut(s) 663
MaeI CTAG 5 cut(s) 929, 1010, 1418, 2009, 2615
MaeII ACGT 7 cut(s) 511, 642, 717, 956, 2559, 2658, 2778
MaeIII GTNAC 7 cut(s) 667, 1366, 1444, 1666, 2123, 2635, 2960
MalI GATC 9 cut(s) 87, 942, 1117, 1719, 2044, 2106, 2704, 2794, 2889
MbiI CCGCTC 1 cut(s) 2077
MboI GATC 9 cut(s) 85, 940, 1115, 1717, 2042, 2104, 2702, 2792, 2887
MfeI CAATTG 1 cut(s) 1935
MflI RGATCY 4 cut(s) 940, 1115, 2042, 2887
MhlI GDGCHC 7 cut(s) 305, 1335, 1393, 1429, 1917, 2625, 2879
MlyI GAGTC 1 cut(s) 1071
MmeI TCCRAC 2 cut(s) 102, 2201
Mph1103I ATGCAT 1 cut(s) 1622
MroXI GAANNNNTTC 2 cut(s) 155, 2496
MslI CAYNNNNRTG 1 cut(s) 1221
MspA1I CMGCKG 3 cut(s) 566, 1328, 2353
MspCI CTTAAG 2 cut(s) 2119, 2129
MspI CCGG 2 cut(s) 1701, 2489
MspR9I CCNGG 6 cut(s) 665, 1346, 1430, 1649, 2193, 2700
MunI CAATTG 1 cut(s) 1935
Mva1269I GAATGC 2 cut(s) 744, 2298
MvaI CCWGG 6 cut(s) 665, 1346, 1430, 1649, 2193, 2700
NdeII GATC 9 cut(s) 85, 940, 1115, 1717, 2042, 2104, 2702, 2792, 2887
NlaIV GGNNCC 6 cut(s) 942, 981, 1117, 1343, 1837, 2664
NmuCI GTSAC 3 cut(s) 667, 1666, 2960
NsiI ATGCAT 1 cut(s) 1622
NspI RCATGY 3 cut(s) 1574, 1624, 2214
NspV TTCGAA 1 cut(s) 2882
PaeI GCATGC 1 cut(s) 1574
PaeR7I CTCGAG 1 cut(s) 1334
PagI TCATGA 1 cut(s) 748
PasI CCCWGGG 1 cut(s) 1429
PciI ACATGT 1 cut(s) 2210
PciSI GCTCTTC 1 cut(s) 293
PctI GAATGC 2 cut(s) 744, 2298
PdmI GAANNNNTTC 2 cut(s) 155, 2496
PfeI GAWTC 6 cut(s) 68, 752, 1151, 1757, 2226, 2271
PflMI CCANNNNNTGG 2 cut(s) 2198, 2699
PinAI ACCGGT 1 cut(s) 1700
PkrI GCNGC 6 cut(s) 65, 148, 465, 2142, 2263, 2764
PleI GAGTC 1 cut(s) 1070
PmaCI CACGTG 1 cut(s) 2560
PmlI CACGTG 1 cut(s) 2560
PpsI GAGTC 1 cut(s) 1070
Ppu21I YACGTR 2 cut(s) 643, 2560
PscI ACATGT 1 cut(s) 2210
Psp124BI GAGCTC 3 cut(s) 305, 1335, 2625
Psp6I CCWGG 6 cut(s) 663, 1344, 1428, 1647, 2191, 2698
PspCI CACGTG 1 cut(s) 2560
PspGI CCWGG 6 cut(s) 663, 1344, 1428, 1647, 2191, 2698
PspN4I GGNNCC 6 cut(s) 942, 981, 1117, 1343, 1837, 2664
PspPI GGNCC 6 cut(s) 74, 628, 790, 1293, 1698, 2627
PspXI VCTCGAGB 1 cut(s) 1334
PstI CTGCAG 3 cut(s) 468, 1639, 2764
PstNI CAGNNNCTG 2 cut(s) 77, 2222
PsuI RGATCY 4 cut(s) 940, 1115, 2042, 2887
PvuII CAGCTG 2 cut(s) 566, 1328
RsaI GTAC 9 cut(s) 254, 482, 503, 639, 959, 1264, 1566, 1980, 2860
RsaNI GTAC 9 cut(s) 253, 481, 502, 638, 958, 1263, 1565, 1979, 2859
RseI CAYNNNNRTG 1 cut(s) 1221
SacI GAGCTC 3 cut(s) 305, 1335, 2625
SapI GCTCTTC 1 cut(s) 293
SatI GCNGC 6 cut(s) 64, 147, 464, 2141, 2262, 2763
Sau3AI GATC 9 cut(s) 85, 940, 1115, 1717, 2042, 2104, 2702, 2792, 2887
Sau96I GGNCC 6 cut(s) 74, 628, 790, 1293, 1698, 2627
ScaI AGTACT 4 cut(s) 503, 1264, 1566, 2860
SchI GAGTC 1 cut(s) 1071
ScrFI CCNGG 6 cut(s) 665, 1346, 1430, 1649, 2193, 2700
SduI GDGCHC 7 cut(s) 305, 1335, 1393, 1429, 1917, 2625, 2879
SexAI ACCWGGT 1 cut(s) 663
SfaNI GCATC 7 cut(s) 1018, 1220, 1358, 1607, 1921, 2677, 2797
SfcI CTRYAG 5 cut(s) 464, 1635, 2056, 2464, 2760
Sfr274I CTCGAG 1 cut(s) 1334
SfuI TTCGAA 1 cut(s) 2882
SinI GGWCC 4 cut(s) 790, 1293, 1698, 2627
SlaI CTCGAG 1 cut(s) 1334
SmiMI CAYNNNNRTG 1 cut(s) 1221
SmlI CTYRAG 6 cut(s) 449, 484, 1334, 1560, 2119, 2129
SmoI CTYRAG 6 cut(s) 449, 484, 1334, 1560, 2119, 2129
SphI GCATGC 1 cut(s) 1574
SsiI CCGC 8 cut(s) 1307, 1710, 2077, 2261, 2353, 2374, 2547, 2908
SspI AATATT 3 cut(s) 421, 1948, 2589
SspMI CTAG 5 cut(s) 929, 1010, 1418, 2009, 2615
SstI GAGCTC 3 cut(s) 305, 1335, 2625
StyD4I CCNGG 6 cut(s) 663, 1344, 1428, 1647, 2191, 2698
StyI CCWWGG 1 cut(s) 2949
TaaI ACNGT 5 cut(s) 156, 231, 1666, 1861, 2301
TaiI ACGT 7 cut(s) 514, 645, 720, 959, 2562, 2661, 2781
TaqI TCGA 5 cut(s) 88, 1335, 1841, 2500, 2882
TatI WGTACW 7 cut(s) 252, 480, 501, 637, 1262, 1564, 2858
TauI GCSGC 1 cut(s) 2264
TfiI GAWTC 6 cut(s) 68, 752, 1151, 1757, 2226, 2271
TscAI CASTG 2 cut(s) 580, 2899
TseFI GTSAC 3 cut(s) 667, 1666, 2960
TseI GCWGC 5 cut(s) 63, 146, 463, 2140, 2762
Tsp45I GTSAC 3 cut(s) 667, 1666, 2960
TspDTI ATGAA 8 cut(s) 737, 1057, 1732, 1767, 1782, 1958, 2581, 2598
TspGWI ACGGA 2 cut(s) 222, 1302
TspRI CASTG 2 cut(s) 580, 2899
Van91I CCANNNNNTGG 2 cut(s) 2198, 2699
Vha464I CTTAAG 2 cut(s) 2119, 2129
VpaK11BI GGWCC 4 cut(s) 790, 1293, 1698, 2627
XagI CCTNNNNNAGG 1 cut(s) 1962
XapI RAATTY 8 cut(s) 52, 280, 333, 472, 702, 1145, 1789, 2080
XbaI TCTAGA 3 cut(s) 928, 1009, 1417
XceI RCATGY 3 cut(s) 1574, 1624, 2214
XcmI CCANNNNNNNNNTGG 1 cut(s) 1924
XhoI CTCGAG 1 cut(s) 1334
XmnI GAANNNNTTC 2 cut(s) 155, 2496
XspI CTAG 5 cut(s) 929, 1010, 1418, 2009, 2615
ZrmI AGTACT 4 cut(s) 503, 1264, 1566, 2860
Zsp2I ATGCAT 1 cut(s) 1622
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.