Prupe.2G042800_v2.0.a1

Retinoblastoma-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0003674
Physical Location & Seq
Reverse (-)
4710441 .. 4718631
8191 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G042800.1

Sequence Viewer

Length: 3081 bp
ATGAGTCCACCTGCTATGGTGAAGATGGAAGATACGAACCCTGAAGTTTCAGCTTCCAACAGCTCAAATTCCGAAAGCAGAGATTCAGACCCCGTCGGAGCTCGATTTACTGATTTGTGCAAGAATGGATTGTCGTTGGATGAGAATCCTTACACGCAAGCTATGAAGCTGTTCAAAGAAACGAAACATCTATTAATATCAAATGCTTCGGCTATTGGAAATGGAACACCTGAAGAAGCAGAAAGGTTCTGGTTTGCCTTTGTTTTGTATTCCGTGAAAACATTGAGTGAGAAGAATTCAGACAATTCTCAGCTGAGCTCTGATGATAATGGTTTTTCTCTCTTCCAAATTTTGAGAGCTGTGAAGCTGAACATTGTAGATTTTTTCAAGGAGCTTCCTCAGTTTGTTGTTAAGGCTGGCCCAATTTTGAGTAATCTTTATGGCATAGATTGGGAAAACAAACTTGAGGCAAAAGAGTTGCAGGCAAATTTTGTGTACTTGAGCCTGTTAAGCAAGTACTACAAACGTGCATACCGAGAATTCTTCTTGACAAGTGATGCAAATGCCGATAAGCAGTCAGCTGTTGCCAGTGGCACTGGCTATGTGTCGGAGTATCATCGTTTTGGATGGTTGCTGTTTTTGGCCCTTCGTGTACACGTATTCAGCCGATTTAAGGACCTGTTGACTTGCGCAAATGGATTGGTTGCCATTTTGGCAATTTTAATTATTCATGTTCCTGTTCGCTTCAGAAAATTCAGCATCCATGATTCAACACACTTTGTGAAGAAAGGCGGCAAAGGTGTGGACCTGCTTGCATCCCTTTGCAACATTTATGACACCTCAGAAGATGAGTTGAGAAATACAATGGAAATGGCTAATGCTTTAATAGCAGACATTCTGAAGAAGAAGCCCTGTTCAGCGTCTGAGTGTAAACATGAAAATCTAGAGAATATGGATCCAGATGGTTTGACCTACTTTGAAGGTCTTATGGAGGAGCCATCATTGTCATCCAGTTTAGATATTCTAGAAAAAGATTATGATGATGCAATTCGGAGTAAGGGTGAACTTGATGAGAGAGTCTTCATCAACGAAGAAGATAGCTTACTTGGTTCAAGGAGCTTTTCTGGAGGATCCATGAATTTATCTGGTGTGAAGAGGAAAGTTGATTCCATTGCCTCCCCAACAAAGACAATTACAAGTCCACTCTCTCCCCATCGCTCTCCTGCATCTCATGTTAATGGTGGTGCTAATTCAAAGATGGTAGCTACCCCTGTGAGCACTGCAATGACAACTGCAAAGTGGCTTCGGACATTTATTTCTCCACTCCCATCGAAACCCTCAACTGAGCTGGAGGGATTCCTGGCATCATGTGATAGGGACGTTACCAATGATGTGATACGCAGGGCACAAGTAATATTGGAGGCTATATTTCCAAGTATTTCTTTGGGAGAACGATGTGTAACTGGTAGCCTGCAAGGTGCTAACCTCATGGACAACATCTGGGCAGAACAAAGGAGATTGGAAGCACTCAAACTTTATTATAGAGTTTTGGAGGCAATGTGTAGGGCAGAGGCTCAAATACTGCATGCAACGAATTTGACCTCTTTATTAACGAATGAGAGGTTCCATAGATGCATGCTAGCCTGCTCTGCAGAGTTAGTCCTGGCAACACATAAGACTGTCACAATGTTGTTTCCAGCAGTATTGGAGAGGACAGGTATTACAGCTTTTGATCTTAGCAAGGTGATAGAGAGCTTCATTAGACATGAGGAATCTCTCCCAAGAGAATTAAGACGACATTTGAATTCATTGGAAGAACGGCTTTTGGAGAGCATGGTATGGGAAAAGGGTTCCTCGATGTATAATTCTTTGACTGTTGCAAGACCAGCTCTCTCAGCAGAAATAAATCGTCTTATGTTATTAGCAGAGCCTATGCCATCTTTGGATGAAATTGCCATGCAAATTAACTTCTCATGTGGAGGGCTGCCTCCGGTACCTTCTTTACAGAAGCATGAGAATTCACCAGGTCAGAATGGAGATATCAGGTCTCCAAAGAGACTCTGCACGGATTATAGAAGTGTATTATTGGAGCGGAATTCCTTTACATCGCCTGTGAAAGATCGTCTTCTGGCCTTCAGTAACCTTAAGTCAAAGCTGCCACCGCCTCCTTTACAGTCTGCATTTGCCAGTCCAACTCGGCCAAATCCAGGAGGTGGAGGGGAAACATGTGCAGAAACTGGAATTAGTATTTTCTTTAGCAAGATAGTCAAGCTGGCGGCTGTCAGAATCAATGGTATGGTTGAAAGGATGCAACTGTCTCAGCAGATCAGAGAGAATGTTTATCGTCTCTTTCAACAAATCCTTGTTCAGCGGACATCTCTCTTTTTTAACCGCCATATCGACCAGATTATTCTTTGTTGTTTTTATGGAGTTGCGAAGATTTCTCAACTTACTCTGACATTTAGGGAAATCATACATAACTATAGAAAGCAACCACAATGTAAATCCCTAGTTTTTCGAAGTGTGTATGTTGATTGGCCTCCAGCACGCCGCAATGGGAGGCCAGCACAGGAACACGTCGATGTCATTACATTTTACAATGAAATATTCATTCCTTCTGTGAAATCTCTGCTTGTTGAGCTAGGATCTGCTGGTCCAACAACCACAAGAGCTAACGTAGTTCCTGAAGCAAATAATAACAATGATGCTCAATGTCCAGGATCACCTAAAGTGTCCACTTTTCCAACTCTCCCTGATATGTCTCCAAAGAAAGTTTCTGCAGCACACAATGTTTATGTCTCTCCATTGCGATCATCTAAGATGGATGCTTTGATCTCGCATAGCTCAAAAAGCTATTATGCTTGTGTTGGAGAGAGCACCCATGCGTATCAAAGCCCTTCAAAAGATCTCACTGCCATTAACAACCGCTTGAATGGTACGCGAAAGCTGAGAGGCACTCTCAACTTCGACAATGTCGATGTGGGGTTGGTCAGTGATTCTATGGTGGCCAACAGCCTCTACCTTCAAAATCAAAATGGGTCATTAGAAGCACCAGTGAAAACTGAGCAACCTGACTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000082 GO:0000278 GO:0000280 GO:0000902 GO:0000904 GO:0001558 GO:0001708 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006275 GO:0006349 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007059 GO:0007127 GO:0007129 GO:0007275 GO:0008134 GO:0008150 GO:0008152 GO:0008356 GO:0009553 GO:0009555 GO:0009566 GO:0009567 GO:0009653 GO:0009791 GO:0009888 GO:0009889 GO:0009987 GO:0010016 GO:0010026 GO:0010052 GO:0010090 GO:0010103 GO:0010374 GO:0010377 GO:0010468 GO:0010556 GO:0010564 GO:0016043 GO:0019219 GO:0019222 GO:0019953 GO:0022402 GO:0022414 GO:0022619 GO:0030154 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032875 GO:0032989 GO:0033043 GO:0034641 GO:0040008 GO:0040029 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0044770 GO:0044772 GO:0044843 GO:0045132 GO:0045143 GO:0045165 GO:0046483 GO:0048229 GO:0048285 GO:0048366 GO:0048367 GO:0048468 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051052 GO:0051128 GO:0051171 GO:0051239 GO:0051276 GO:0051301 GO:0051321 GO:0051704 GO:0051726 GO:0051783 GO:0055046 GO:0060255 GO:0061982 GO:0065007 GO:0070192 GO:0071514 GO:0071704 GO:0071840 GO:0080090 GO:0090329 GO:0090558 GO:0090626 GO:0090627 GO:0090698 GO:0097159 GO:0098813 GO:0099402 GO:0140013 GO:1901360 GO:1901363 GO:1903046 GO:1903047 GO:1903866 GO:2000036 GO:2000112 GO:2000653
KEGG Pathways
Metabolic & Signaling
ko04110 ko04218 ko04350 ko05165 ko05203 map04110 map04218 map04350 map05165 map05203
Pfam Domains
Protein Families
DUF3452 RB_A RB_B Rb_C

Protein Analysis

1027

Amino Acids

114.15

Weight (kDa)

7.78

Isoelectric Point (pI)

49.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 19
Acc16I TGCGCA 1 cut(s) 691
Acc36I ACCTGC 2 cut(s) 19, 816
Acc65I GGTACC 1 cut(s) 1993
AccB1I GGYRCC 1 cut(s) 1993
AccB7I CCANNNNNTGG 1 cut(s) 2213
AccBSI CCGCTC 1 cut(s) 2092
AccII CGCG 1 cut(s) 2941
AciI CCGC 8 cut(s) 792, 2092, 2162, 2276, 2371, 2392, 2551, 2926
AclWI GGATC 6 cut(s) 950, 963, 1125, 1138, 2653, 2728
AcoI YGGCCR 2 cut(s) 2198, 3006
AcuI CTGAAG 6 cut(s) 63, 252, 730, 920, 2119, 2706
AdeI CACNNNGTG 1 cut(s) 781
AfaI GTAC 5 cut(s) 497, 518, 654, 1995, 2938
AfiI CCNNNNNNNGG 3 cut(s) 673, 2207, 2213
AflII CTTAAG 1 cut(s) 2144
AflIII ACRYGT 3 cut(s) 655, 2225, 2575
AjiI CACGTC 1 cut(s) 2578
AjnI CCWGG 5 cut(s) 1359, 1662, 2023, 2206, 2716
AjuI GAANNNNNNNTTGG 2 cut(s) 2758, 2790
Alw21I GWGCWC 4 cut(s) 103, 320, 1280, 2879
Alw26I GTCTC 6 cut(s) 2050, 2052, 2322, 2351, 2766, 2803
AlwI GGATC 6 cut(s) 950, 963, 1125, 1138, 2653, 2728
AlwNI CAGNNNCTG 2 cut(s) 923, 2237
AoxI GGCC 7 cut(s) 418, 642, 2130, 2198, 2537, 2561, 3006
ApeKI GCWGC 3 cut(s) 1984, 2155, 2780
AseI ATTAAT 1 cut(s) 194
Asp700I GAANNNNTTC 1 cut(s) 170
Asp718I GGTACC 1 cut(s) 1993
AspLEI GCGC 1 cut(s) 692
AspS9I GGNCC 5 cut(s) 419, 643, 676, 805, 2654
AsuHPI GGTGA 5 cut(s) 31, 1073, 1756, 2013, 2715
AsuII TTCGAA 1 cut(s) 2518
AsuNHI GCTAGC 1 cut(s) 1639
AvaII GGWCC 3 cut(s) 676, 805, 2654
BaeGI GKGCMC 1 cut(s) 1408
BalI TGGCCA 1 cut(s) 3008
BamHI GGATCC 2 cut(s) 955, 1130
BanI GGYRCC 1 cut(s) 1993
BanII GRGCYC 2 cut(s) 103, 320
BarI GAAGNNNNNNTAC 2 cut(s) 1086, 1118
BbsI GAAGAC 2 cut(s) 1072, 2117
Bbv12I GWGCWC 4 cut(s) 103, 320, 1280, 2879
BbvI GCAGC 3 cut(s) 1971, 2142, 2792
BccI CCATC 9 cut(s) 19, 621, 956, 1006, 1221, 1252, 1336, 1945, 2815
BceAI ACGGC 1 cut(s) 1835
BciT130I CCWGG 5 cut(s) 1361, 1664, 2025, 2208, 2718
BcoDI GTCTC 6 cut(s) 2050, 2052, 2322, 2351, 2766, 2803
BfaI CTAG 5 cut(s) 944, 1025, 1640, 2510, 2642
BfmI CTRYAG 3 cut(s) 1650, 2482, 2778
BfrI CTTAAG 1 cut(s) 2144
BfuAI ACCTGC 2 cut(s) 19, 816
BglI GCCNNNNNGGC 1 cut(s) 713
BglII AGATCT 1 cut(s) 2905
BisI GCNGC 6 cut(s) 793, 1985, 2156, 2277, 2551, 2781
BlpI GCTNAGC 1 cut(s) 314
BlsI GCNGC 6 cut(s) 794, 1986, 2157, 2278, 2552, 2782
BmcAI AGTACT 1 cut(s) 518
Bme1390I CCNGG 5 cut(s) 1361, 1664, 2025, 2208, 2718
Bme18I GGWCC 3 cut(s) 676, 805, 2654
BmgBI CACGTC 1 cut(s) 2578
BmgT120I GGNCC 5 cut(s) 419, 643, 676, 805, 2654
BmiI GGNNCC 6 cut(s) 957, 996, 1132, 1625, 1852, 1995
BmrFI CCNGG 5 cut(s) 1361, 1664, 2025, 2208, 2718
BmtI GCTAGC 1 cut(s) 1643
BpiI GAAGAC 2 cut(s) 1072, 2117
BplI GAGNNNNNCTC 4 cut(s) 2941, 2973, 2943, 2975
BpmI CTGGAG 3 cut(s) 1146, 1370, 2526
Bpu1102I GCTNAGC 1 cut(s) 314
Bpu14I TTCGAA 1 cut(s) 2518
BpuEI CTTGAG 2 cut(s) 485, 520
BsaAI YACGTR 1 cut(s) 658
BsaBI GATNNNNATC 1 cut(s) 144
BsaI GGTCTC 1 cut(s) 2052
BsaWI WCCGGW 1 cut(s) 1990
BsaXI ACNNNNNCTCC 4 cut(s) 1045, 1075, 1544, 1574
Bsc4I CCNNNNNNNGG 3 cut(s) 673, 2207, 2213
Bse1I ACTGG 7 cut(s) 588, 601, 1011, 1468, 2187, 2242, 3053
Bse3DI GCAATG 5 cut(s) 1170, 1290, 1563, 2560, 2804
Bse8I GATNNNNATC 1 cut(s) 144
BseBI CCWGG 5 cut(s) 1361, 1664, 2025, 2208, 2718
BseGI GGATG 8 cut(s) 145, 632, 759, 815, 1007, 1951, 2313, 2830
BseJI GATNNNNATC 1 cut(s) 144
BseLI CCNNNNNNNGG 3 cut(s) 673, 2207, 2213
BseMI GCAATG 5 cut(s) 1170, 1290, 1563, 2560, 2804
BseNI ACTGG 7 cut(s) 588, 601, 1011, 1468, 2187, 2242, 3053
BseRI GAGGAG 1 cut(s) 1007
BseSI GKGCMC 1 cut(s) 1408
BseXI GCAGC 3 cut(s) 1971, 2142, 2792
BsgI GTGCAG 2 cut(s) 2047, 2250
Bsh1236I CGCG 1 cut(s) 2941
BshFI GGCC 7 cut(s) 420, 644, 2132, 2200, 2539, 2563, 3008
BshNI GGYRCC 1 cut(s) 1993
BsiHKAI GWGCWC 4 cut(s) 103, 320, 1280, 2879
BsiSI CCGG 1 cut(s) 1991
BslFI GGGAC 1 cut(s) 1391
BslI CCNNNNNNNGG 3 cut(s) 673, 2207, 2213
BsmAI GTCTC 6 cut(s) 2050, 2052, 2322, 2351, 2766, 2803
BsmBI CGTCTC 1 cut(s) 2351
BsmFI GGGAC 1 cut(s) 1391
BsnI GGCC 7 cut(s) 420, 644, 2132, 2200, 2539, 2563, 3008
Bso31I GGTCTC 1 cut(s) 2052
Bsp119I TTCGAA 1 cut(s) 2518
Bsp1286I GDGCHC 5 cut(s) 103, 320, 1280, 1408, 2879
Bsp1407I TGTACA 1 cut(s) 652
Bsp1720I GCTNAGC 1 cut(s) 314
BspACI CCGC 8 cut(s) 792, 2092, 2162, 2276, 2371, 2392, 2551, 2926
BspANI GGCC 7 cut(s) 420, 644, 2132, 2200, 2539, 2563, 3008
BspFNI CGCG 1 cut(s) 2941
BspLI GGNNCC 6 cut(s) 957, 996, 1132, 1625, 1852, 1995
BspMAI CTGCAG 2 cut(s) 1654, 2782
BspMI ACCTGC 2 cut(s) 19, 816
BspOI GCTAGC 1 cut(s) 1643
BspPI GGATC 6 cut(s) 950, 963, 1125, 1138, 2653, 2728
BspT104I TTCGAA 1 cut(s) 2518
BspT107I GGYRCC 1 cut(s) 1993
BspTI CTTAAG 1 cut(s) 2144
BspTNI GGTCTC 1 cut(s) 2052
BsrBI CCGCTC 1 cut(s) 2092
BsrDI GCAATG 5 cut(s) 1170, 1290, 1563, 2560, 2804
BsrGI TGTACA 1 cut(s) 652
BsrI ACTGG 7 cut(s) 588, 601, 1011, 1468, 2187, 2242, 3053
Bst2UI CCWGG 5 cut(s) 1361, 1664, 2025, 2208, 2718
Bst4CI ACNGT 4 cut(s) 1681, 1876, 2175, 2316
Bst6I CTCTTC 2 cut(s) 347, 1148
BstAFI CTTAAG 1 cut(s) 2144
BstAUI TGTACA 1 cut(s) 652
BstBAI YACGTR 1 cut(s) 658
BstBI TTCGAA 1 cut(s) 2518
BstF5I GGATG 8 cut(s) 145, 632, 759, 815, 1007, 1951, 2313, 2830
BstFNI CGCG 1 cut(s) 2941
BstHHI GCGC 1 cut(s) 692
BstMAI GTCTC 6 cut(s) 2050, 2052, 2322, 2351, 2766, 2803
BstMWI GCNNNNNNNGC 9 cut(s) 510, 713, 887, 1649, 1886, 1895, 2161, 2638, 2850
BstNI CCWGG 5 cut(s) 1361, 1664, 2025, 2208, 2718
BstNSI RCATGY 3 cut(s) 1589, 1639, 2229
BstSCI CCNGG 5 cut(s) 1359, 1662, 2023, 2206, 2716
BstSFI CTRYAG 3 cut(s) 1650, 2482, 2778
BstSLI GKGCMC 1 cut(s) 1408
BstUI CGCG 1 cut(s) 2941
BstV1I GCAGC 3 cut(s) 1971, 2142, 2792
BstV2I GAAGAC 2 cut(s) 1072, 2117
BstX2I RGATCY 4 cut(s) 955, 1130, 2645, 2905
BstYI RGATCY 4 cut(s) 955, 1130, 2645, 2905
BsuRI GGCC 7 cut(s) 420, 644, 2132, 2200, 2539, 2563, 3008
BtgZI GCGATG 2 cut(s) 1199, 2091
BtrI CACGTC 1 cut(s) 2578
BtsCI GGATG 8 cut(s) 145, 632, 759, 815, 1007, 1951, 2313, 2830
BtsI GCAGTG 2 cut(s) 1278, 2910
BtsIMutI CAGTG 6 cut(s) 594, 595, 1278, 2910, 2998, 3060
BveI ACCTGC 2 cut(s) 19, 816
CaiI CAGNNNCTG 2 cut(s) 923, 2237
CfoI GCGC 1 cut(s) 692
Cfr13I GGNCC 5 cut(s) 419, 643, 676, 805, 2654
CseI GACGC 1 cut(s) 909
CsiI ACCWGGT 1 cut(s) 2023
Csp6I GTAC 5 cut(s) 496, 517, 653, 1994, 2937
CviQI GTAC 5 cut(s) 496, 517, 653, 1994, 2937
DraIII CACNNNGTG 1 cut(s) 781
EaeI YGGCCR 2 cut(s) 2198, 3006
Eam1104I CTCTTC 2 cut(s) 347, 1148
EarI CTCTTC 2 cut(s) 347, 1148
Ecl136II GAGCTC 2 cut(s) 101, 318
Eco24I GRGCYC 2 cut(s) 103, 320
Eco31I GGTCTC 1 cut(s) 2052
Eco32I GATATC 1 cut(s) 2041
Eco47I GGWCC 3 cut(s) 676, 805, 2654
Eco53kI GAGCTC 2 cut(s) 101, 318
Eco57I CTGAAG 6 cut(s) 63, 252, 730, 920, 2119, 2706
EcoICRI GAGCTC 2 cut(s) 101, 318
EcoO109I RGGNCCY 1 cut(s) 676
EcoRI GAATTC 5 cut(s) 295, 539, 1804, 2017, 2095
EcoRII CCWGG 5 cut(s) 1359, 1662, 2023, 2206, 2716
EcoRV GATATC 1 cut(s) 2041
EcoT22I ATGCAT 1 cut(s) 1637
EcoT38I GRGCYC 2 cut(s) 103, 320
Esp3I CGTCTC 1 cut(s) 2351
FalI AAGNNNNNCTT 6 cut(s) 1426, 1458, 1806, 1838, 2109, 2141
FaqI GGGAC 1 cut(s) 1391
Fnu4HI GCNGC 6 cut(s) 793, 1985, 2156, 2277, 2551, 2781
FokI GGATG 8 cut(s) 152, 639, 746, 802, 994, 1958, 2320, 2837
FriOI GRGCYC 2 cut(s) 103, 320
Fsp4HI GCNGC 6 cut(s) 793, 1985, 2156, 2277, 2551, 2781
FspBI CTAG 5 cut(s) 944, 1025, 1640, 2510, 2642
FspI TGCGCA 1 cut(s) 691
GlaI GCGC 1 cut(s) 691
GluI GCNGC 6 cut(s) 793, 1985, 2156, 2277, 2551, 2781
GsuI CTGGAG 3 cut(s) 1146, 1370, 2526
HaeIII GGCC 7 cut(s) 420, 644, 2132, 2200, 2539, 2563, 3008
HapII CCGG 1 cut(s) 1991
HgaI GACGC 1 cut(s) 909
HhaI GCGC 1 cut(s) 692
Hin6I GCGC 1 cut(s) 690
HinP1I GCGC 1 cut(s) 690
HincII GTYRAC 1 cut(s) 684
HindII GTYRAC 1 cut(s) 684
HpaII CCGG 1 cut(s) 1991
HphI GGTGA 5 cut(s) 31, 1073, 1756, 2013, 2715
Hpy188III TCNNGA 6 cut(s) 547, 944, 959, 1025, 1125, 2684
Hpy99I CGWCG 2 cut(s) 98, 2582
HpyAV CCTTC 7 cut(s) 656, 974, 2007, 2143, 2625, 2907, 3032
HpyCH4III ACNGT 4 cut(s) 1681, 1876, 2175, 2316
HpyCH4IV ACGT 5 cut(s) 526, 657, 1380, 2577, 2676
HpyF10VI GCNNNNNNNGC 9 cut(s) 510, 713, 887, 1649, 1886, 1895, 2161, 2638, 2850
HpySE526I ACGT 5 cut(s) 526, 657, 1380, 2577, 2676
HspAI GCGC 1 cut(s) 690
KpnI GGTACC 1 cut(s) 1997
LmnI GCTCC 5 cut(s) 98, 391, 994, 1116, 2089
Lsp1109I GCAGC 3 cut(s) 1971, 2142, 2792
MabI ACCWGGT 1 cut(s) 2023
MaeI CTAG 5 cut(s) 944, 1025, 1640, 2510, 2642
MaeII ACGT 5 cut(s) 526, 657, 1380, 2577, 2676
MaeIII GTNAC 4 cut(s) 1381, 1459, 1681, 2138
MbiI CCGCTC 1 cut(s) 2092
MflI RGATCY 4 cut(s) 955, 1130, 2645, 2905
MhlI GDGCHC 5 cut(s) 103, 320, 1280, 1408, 2879
MlsI TGGCCA 1 cut(s) 3008
MluNI TGGCCA 1 cut(s) 3008
MlyI GAGTC 3 cut(s) 13, 1086, 2052
MmeI TCCRAC 8 cut(s) 76, 81, 117, 588, 2216, 2681, 2768, 2848
Mox20I TGGCCA 1 cut(s) 3008
Mph1103I ATGCAT 1 cut(s) 1637
MroXI GAANNNNTTC 1 cut(s) 170
MscI TGGCCA 1 cut(s) 3008
MslI CAYNNNNRTG 3 cut(s) 1236, 1283, 2580
Msp20I TGGCCA 1 cut(s) 3008
MspA1I CMGCKG 3 cut(s) 313, 581, 2371
MspCI CTTAAG 1 cut(s) 2144
MspI CCGG 1 cut(s) 1991
MspR9I CCNGG 5 cut(s) 1361, 1664, 2025, 2208, 2718
MvaI CCWGG 5 cut(s) 1361, 1664, 2025, 2208, 2718
MvnI CGCG 1 cut(s) 2941
MwoI GCNNNNNNNGC 9 cut(s) 510, 713, 887, 1649, 1886, 1895, 2161, 2638, 2850
NheI GCTAGC 1 cut(s) 1639
NlaIV GGNNCC 6 cut(s) 957, 996, 1132, 1625, 1852, 1995
NmeAIII GCCGAG 1 cut(s) 2176
NmuCI GTSAC 1 cut(s) 1681
NsbI TGCGCA 1 cut(s) 691
NsiI ATGCAT 1 cut(s) 1637
NspI RCATGY 3 cut(s) 1589, 1639, 2229
NspV TTCGAA 1 cut(s) 2518
PaeI GCATGC 2 cut(s) 1589, 1639
PaqCI CACCTGC 1 cut(s) 19
PciI ACATGT 1 cut(s) 2225
PcsI WCGNNNNNNNCGW 2 cut(s) 532, 2973
PdmI GAANNNNTTC 1 cut(s) 170
PfeI GAWTC 8 cut(s) 83, 145, 767, 1166, 1356, 1772, 2286, 2996
PflFI GACNNNGTC 2 cut(s) 92, 2972
PflMI CCANNNNNTGG 1 cut(s) 2213
PfoI TCCNGGA 2 cut(s) 2206, 2716
PkrI GCNGC 6 cut(s) 794, 1986, 2157, 2278, 2552, 2782
PleI GAGTC 3 cut(s) 12, 1085, 2052
PpsI GAGTC 3 cut(s) 12, 1085, 2052
Ppu21I YACGTR 1 cut(s) 658
PpuMI RGGWCCY 1 cut(s) 676
PscI ACATGT 1 cut(s) 2225
PshBI ATTAAT 1 cut(s) 194
Psp124BI GAGCTC 2 cut(s) 103, 320
Psp5II RGGWCCY 1 cut(s) 676
Psp6I CCWGG 5 cut(s) 1359, 1662, 2023, 2206, 2716
PspGI CCWGG 5 cut(s) 1359, 1662, 2023, 2206, 2716
PspN4I GGNNCC 6 cut(s) 957, 996, 1132, 1625, 1852, 1995
PspPI GGNCC 5 cut(s) 419, 643, 676, 805, 2654
PspPPI RGGWCCY 1 cut(s) 676
PstI CTGCAG 2 cut(s) 1654, 2782
PstNI CAGNNNCTG 2 cut(s) 923, 2237
PsuI RGATCY 4 cut(s) 955, 1130, 2645, 2905
PsyI GACNNNGTC 2 cut(s) 92, 2972
PvuII CAGCTG 2 cut(s) 313, 581
RsaI GTAC 5 cut(s) 497, 518, 654, 1995, 2938
RsaNI GTAC 5 cut(s) 496, 517, 653, 1994, 2937
RseI CAYNNNNRTG 3 cut(s) 1236, 1283, 2580
SacI GAGCTC 2 cut(s) 103, 320
SatI GCNGC 6 cut(s) 793, 1985, 2156, 2277, 2551, 2781
Sau96I GGNCC 5 cut(s) 419, 643, 676, 805, 2654
ScaI AGTACT 1 cut(s) 518
SchI GAGTC 3 cut(s) 13, 1086, 2052
ScrFI CCNGG 5 cut(s) 1361, 1664, 2025, 2208, 2718
SduI GDGCHC 5 cut(s) 103, 320, 1280, 1408, 2879
SexAI ACCWGGT 1 cut(s) 2023
SfcI CTRYAG 3 cut(s) 1650, 2482, 2778
SfuI TTCGAA 1 cut(s) 2518
SinI GGWCC 3 cut(s) 676, 805, 2654
SmiMI CAYNNNNRTG 3 cut(s) 1236, 1283, 2580
SmlI CTYRAG 3 cut(s) 464, 499, 2144
SmoI CTYRAG 3 cut(s) 464, 499, 2144
SphI GCATGC 2 cut(s) 1589, 1639
SsiI CCGC 8 cut(s) 792, 2092, 2162, 2276, 2371, 2392, 2551, 2926
SspI AATATT 2 cut(s) 1416, 2607
SspMI CTAG 5 cut(s) 944, 1025, 1640, 2510, 2642
SstI GAGCTC 2 cut(s) 103, 320
StyD4I CCNGG 5 cut(s) 1359, 1662, 2023, 2206, 2716
TaaI ACNGT 4 cut(s) 1681, 1876, 2175, 2316
TaiI ACGT 5 cut(s) 529, 660, 1383, 2580, 2679
TaqI TCGA 8 cut(s) 103, 1331, 1856, 2400, 2518, 2580, 2967, 2976
TatI WGTACW 3 cut(s) 495, 516, 652
TauI GCSGC 3 cut(s) 795, 2279, 2553
TfiI GAWTC 8 cut(s) 83, 145, 767, 1166, 1356, 1772, 2286, 2996
TscAI CASTG 6 cut(s) 595, 601, 1285, 2917, 2998, 3060
TseFI GTSAC 1 cut(s) 1681
TseI GCWGC 3 cut(s) 1984, 2155, 2780
Tsp45I GTSAC 1 cut(s) 1681
TspGWI ACGGA 2 cut(s) 262, 2081
TspRI CASTG 6 cut(s) 595, 601, 1285, 2917, 2998, 3060
Tth111I GACNNNGTC 2 cut(s) 92, 2972
Van91I CCANNNNNTGG 1 cut(s) 2213
Vha464I CTTAAG 1 cut(s) 2144
VpaK11BI GGWCC 3 cut(s) 676, 805, 2654
VspI ATTAAT 1 cut(s) 194
XbaI TCTAGA 2 cut(s) 943, 1024
XceI RCATGY 3 cut(s) 1589, 1639, 2229
XmnI GAANNNNTTC 1 cut(s) 170
XspI CTAG 5 cut(s) 944, 1025, 1640, 2510, 2642
ZrmI AGTACT 1 cut(s) 518
Zsp2I ATGCAT 1 cut(s) 1637
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.