Rh1AG077400

Retinoblastoma-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0003674
Physical Location & Seq
Reverse (-)
12997163 .. 13005521
8359 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG077400.1

Sequence Viewer

Length: 2829 bp
ATGAGTCCACCTGCTGTGCTGAACATGGAAGATACGAAGCCGGAGGTCTCTGCATCCAACAATTCGAATTCGGGAACTGGAGATTTGGACCCTGTTAAAGCTCGATTTACTGATTTGTGCAAGAACGGATTGTCGTTGGACGAGAACACGCTGACGCAAGCTATCAAGCTGCTGATAGAAACGAAACATGTTCTAATATCAAATTTATCGGCTATCGGAAATGGAACGCCTGAAGAAGCAGAGCGGTTCTGGTTTTCGTTTGTTTTGTTCTCGGTGAAAACATTGAGTGAGAAGAGTTCAGATAATTCGCTAAAGGGGTCTGATGATGGTGGATTCTCTCTCATCCAAATTTTGAGAGCTGCTAAGCTGAACATTGTGGATTTCTTTAAGGAGCTTCCACAGTTTATTGTTAAGGCCGGCCCAATTTTAAGTAATCTGTATGGCGTAGATTGGGAGAGCAAACTTGAGGCAAAAGAGTTGCAGGCCAATTTTCTGTACTTGAGCCTTTTAAGCAAGTACTTCAAAAAAGCATACCGGGAATTCTTCTTGACAAGTGATGCAACTGTTGATAAGAAGTCAACCATTACCAATGGAGCTGGCTATGTGTCAGAGTATCATAGATTTGGATGGCTGCTGTTTTTGGCCCTTCGGGTACATGTATTTGGGCGAGTTAAAGACCTGGTGACATGCACAAACGGCTTGGTTTCCATATTGGCAATTTTAATTATTCACGTCCCTGTTCGCTTCAGAAACTTTAGAATCCATGATTCACCACGTTTTGTGAGGAAAGGCAACAAAGGTGTGGACCTGCTTGCATCACTTTGCAACACTTATGACACCTCAGAAGAGGAGTTGAGAAAAACAATGGAAAAGGCTAATACATTGATATCAGATCTTTTGAAGAAGAAGCCTTGTTTGGCATCTGAGTGTAAAAATGAAAATCTAAAGAATATTGATACAGATGGTTTGACCTATTTTGAAGGACTTCTGGATGAACCTTCTTTGTCATCCAGTTTAGATATTCTAGAAAAAGATCATGATGATGCAATTCGGAATAAGGGTGAACTAGATGAGAGAGTATTTATAAATGAGGAGGACAGCTTACTTGGTTCAGGGAGCTTGTCAGGAGGGTCCTTGAATTTATCTGGTGTGAAGAGGAAAATTGATTCAATGGCCTCCCCAACAAAGACAATTACAAGTCCACTCTCTCCCCATCGCTCTCCAGCCTCGCATGTTAATGGTGGTGTTAATTCAAAGATGGCAGCTACACCTGTGAGCACTGCAATGACAACTGCAAAGTGGCTTAGGACCTTTATTTCCCCACTTCCATCAAAACCCTCACCCGAGCTCGAGGGTTTTCTGGCATCATGTGATAGGAACGTTAGTAATGATGTGATACGCAGGGCGCAAATAATACTTGAGGCCATATTTCCAAGTAGTGCTTTGGGGGAGCGATGTGTAACTGGTAGTCTGCAAAGTGCTAACCTCATGGACAATGTGTGGGCAGAACAACGAAGACTGGAAGCACTCAAGCTTTATTATAGAGTTTTGGAAGCAATGTGTAGGGCAGAGGCTCAAGTTCTGCATGCTACTAATTTGACCTCTTTATTAACAAATGAGAGGTTCCATAGATGCATGCTAGCATGCTCTGCTGAGTTAGTCCTGGCAACACATAAGACGGTCACAATGTTGTTTCCTGCCGTACTAGAAAGGACAGGCATTACAGCTTTTGATCTTAGCAAGGTGATAGAGAGCTTCATTAGACATGAAGAATCTCTACCAAGAGAATTGAGACGACATCTAAATTCATTGGAAGAACGACTTTTGGAGAGCATGGTATGGGAGAAGGGTTCCTCAATGTACAATTCTTTGACAGTTGCTAGGCCTGCCTTCTCGGCTGAAATCAACCGTCTTGGGTTATTAGCAGAACCAATGCCATCTTTGGATGCAATAGCTTTGCATATCAACTTCTCTTGTGGAGGGTTGCCTCCAGTAGCTTCTTTACAGAAGCATGAGACTTCCCCAGGTCAGAATGGAGATCTCAGGTCTCCGAAGAGACCCTGCACAGAATATAGGGGTGTATTACTGGAGCGAAATTCCTTTACTTCTCCAGTGAAGGATCGTCTCCTGGCCTTAAGTAACCTTAAATCAAAGCTGCCACCGCCACCTCTACAATCTGCATTTGCAAGTCCAACACGACCAAATCCAGGAGGTGGAGGGGAAACATGTGCAGAAACTGGAGTTAGTATTTTCTTTAATAAGATCATCAAGCTGGCTGCTGTAAGAATTAATGGTATGGTCGAAAGGTTGCAATTGTCTCAGCAAATCAGGGAGAATGTTTATCGCCTCTTCCAACAAATACTTGTTCAGCGGACATCTCTCTTTTTTAACCGCCATATTGACCAAATTATCCTTTGTTGTTTTTACGGGGTTGCCAAGATTTCTCAACTAAGTCTTACATTTAGGGAAATCATACACAACTACAGGAAGCAACCACAATGCAAACCACAAGTTTTTCGAAGTGTCTTTGTTGACTGGTCATCAGCTAGACGCAATGGGCGGCCTGGACAGGAACACGTAGATATCATTAGATTTTACAATGAAGTATTCATTCCTTCTGTCAAGACCTTGCTTGTTGAGCTTGGGCCAGCTGTTACAACTACAAAAGCCAATGTAGTTCCTGAAGCTAACAACAACAATGATGCTCAATGTCCAGGATCACCTAAAGTATCCACCTTTCCAAGTCTCCCTGATATGTCTCCAAAGAAAGTATCTGCAGCACACAATGTTTATGTATCTCCATTGCGATCATCAAAGGTACTTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000082 GO:0000278 GO:0000280 GO:0000902 GO:0000904 GO:0001558 GO:0001708 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006275 GO:0006349 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007059 GO:0007127 GO:0007129 GO:0007275 GO:0008134 GO:0008150 GO:0008152 GO:0008356 GO:0009553 GO:0009555 GO:0009566 GO:0009567 GO:0009653 GO:0009791 GO:0009888 GO:0009889 GO:0009987 GO:0010016 GO:0010026 GO:0010052 GO:0010090 GO:0010103 GO:0010374 GO:0010377 GO:0010468 GO:0010556 GO:0010564 GO:0016043 GO:0019219 GO:0019222 GO:0019953 GO:0022402 GO:0022414 GO:0022619 GO:0030154 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032875 GO:0032989 GO:0033043 GO:0034641 GO:0040008 GO:0040029 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0044770 GO:0044772 GO:0044843 GO:0045132 GO:0045143 GO:0045165 GO:0046483 GO:0048229 GO:0048285 GO:0048366 GO:0048367 GO:0048468 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051052 GO:0051128 GO:0051171 GO:0051239 GO:0051276 GO:0051301 GO:0051321 GO:0051704 GO:0051726 GO:0051783 GO:0055046 GO:0060255 GO:0061982 GO:0065007 GO:0070192 GO:0071514 GO:0071704 GO:0071840 GO:0080090 GO:0090329 GO:0090558 GO:0090626 GO:0090627 GO:0090698 GO:0097159 GO:0098813 GO:0099402 GO:0140013 GO:1901360 GO:1901363 GO:1903046 GO:1903047 GO:1903866 GO:2000036 GO:2000112 GO:2000653
KEGG Pathways
Metabolic & Signaling
ko04110 ko04218 ko04350 ko05165 ko05203 map04110 map04218 map04350 map05165 map05203
Pfam Domains
Protein Families
DUF3452 RB_A RB_B Rb_C

Protein Analysis

942

Amino Acids

104.54

Weight (kDa)

8.86

Isoelectric Point (pI)

47.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3452 PF11934 108 - 244 4e-33 Domain of unknown function (DUF3452)
RB_A PF01858 423 - 624 2.1e-64 Retinoblastoma-associated protein A domain
RB_B PF01857 748 - 875 1.9e-43 Retinoblastoma-associated protein B domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1085
AarI CACCTGC 1 cut(s) 19
Acc36I ACCTGC 2 cut(s) 19, 816
AccB7I CCANNNNNTGG 1 cut(s) 2213
AccBSI CCGCTC 1 cut(s) 244
AciI CCGC 5 cut(s) 244, 2162, 2371, 2392, 2560
AclI AACGTT 1 cut(s) 1380
AclWI GGATC 2 cut(s) 2127, 2725
AcsI RAATTY 7 cut(s) 67, 202, 348, 539, 1138, 1804, 2095
AcuI CTGAAG 3 cut(s) 252, 730, 2703
AfaI GTAC 6 cut(s) 497, 518, 654, 1704, 1862, 2820
AfiI CCNNNNNNNGG 2 cut(s) 2207, 2213
AflII CTTAAG 1 cut(s) 2134
AflIII ACRYGT 4 cut(s) 187, 655, 2225, 2575
AgsI TTSAA 6 cut(s) 523, 901, 980, 1138, 1170, 1254
AjiI CACGTC 1 cut(s) 733
AjnI CCWGG 7 cut(s) 678, 1662, 2023, 2127, 2206, 2563, 2713
AjuI GAANNNNNNNTTGG 2 cut(s) 899, 931
Alw21I GWGCWC 2 cut(s) 1280, 1350
Alw26I GTCTC 9 cut(s) 52, 1786, 2009, 2050, 2052, 2129, 2322, 2750, 2763
AlwI GGATC 2 cut(s) 2127, 2725
AlwNI CAGNNNCTG 1 cut(s) 2237
Ama87I CYCGRG 2 cut(s) 1343, 1349
ApeKI GCWGC 7 cut(s) 169, 359, 631, 1262, 2155, 2276, 2777
ApoI RAATTY 7 cut(s) 67, 202, 348, 539, 1138, 1804, 2095
AseI ATTAAT 1 cut(s) 2289
Asp700I GAANNNNTTC 1 cut(s) 984
AspLEI GCGC 1 cut(s) 1408
AspS9I GGNCC 7 cut(s) 88, 419, 643, 805, 1131, 1308, 2645
AsuC2I CCSGG 1 cut(s) 536
AsuHPI GGTGA 7 cut(s) 286, 694, 762, 1073, 1332, 1756, 2712
AsuII TTCGAA 2 cut(s) 65, 2518
AsuNHI GCTAGC 1 cut(s) 1639
AvaI CYCGRG 2 cut(s) 1343, 1349
AvaII GGWCC 4 cut(s) 88, 805, 1131, 1308
BanII GRGCYC 1 cut(s) 1350
BarI GAAGNNNNNNTAC 2 cut(s) 1761, 1793
BbsI GAAGAC 1 cut(s) 1522
Bbv12I GWGCWC 2 cut(s) 1280, 1350
BbvI GCAGC 7 cut(s) 156, 346, 618, 1274, 2142, 2263, 2789
BccI CCATC 7 cut(s) 320, 621, 956, 1221, 1252, 1336, 1945
BceAI ACGGC 2 cut(s) 712, 1685
BciT130I CCWGG 7 cut(s) 680, 1664, 2025, 2129, 2208, 2565, 2715
BciVI GTATCC 1 cut(s) 2740
BcnI CCSGG 1 cut(s) 536
BcoDI GTCTC 9 cut(s) 52, 1786, 2009, 2050, 2052, 2129, 2322, 2750, 2763
BfaI CTAG 6 cut(s) 1025, 1067, 1640, 1706, 1881, 2547
BfmI CTRYAG 2 cut(s) 2482, 2775
BfrI CTTAAG 1 cut(s) 2134
BfuAI ACCTGC 2 cut(s) 19, 816
BfuI GTATCC 1 cut(s) 2740
BglI GCCNNNNNGGC 1 cut(s) 1895
BglII AGATCT 2 cut(s) 892, 2038
BisI GCNGC 8 cut(s) 170, 360, 632, 1263, 2156, 2277, 2561, 2778
BlpI GCTNAGC 1 cut(s) 363
BlsI GCNGC 8 cut(s) 171, 361, 633, 1264, 2157, 2278, 2562, 2779
BmcAI AGTACT 1 cut(s) 518
Bme1390I CCNGG 8 cut(s) 536, 680, 1664, 2025, 2129, 2208, 2565, 2715
Bme18I GGWCC 4 cut(s) 88, 805, 1131, 1308
BmeT110I CYCGRG 2 cut(s) 1343, 1349
BmgBI CACGTC 1 cut(s) 733
BmgT120I GGNCC 7 cut(s) 88, 419, 643, 805, 1131, 1308, 2645
BmiI GGNNCC 4 cut(s) 90, 1132, 1625, 1852
BmrFI CCNGG 8 cut(s) 536, 680, 1664, 2025, 2129, 2208, 2565, 2715
BmsI GCATC 9 cut(s) 62, 547, 824, 929, 1033, 1373, 1622, 1936, 2692
BmtI GCTAGC 1 cut(s) 1643
BpiI GAAGAC 1 cut(s) 1522
BpmI CTGGAG 6 cut(s) 99, 1206, 1974, 2094, 2108, 2259
Bpu10I CCTNAGC 1 cut(s) 1304
Bpu1102I GCTNAGC 1 cut(s) 363
Bpu14I TTCGAA 2 cut(s) 65, 2518
BpuEI CTTGAG 5 cut(s) 485, 520, 1439, 1514, 1560
BpuMI CCSGG 1 cut(s) 536
BsaAI YACGTR 1 cut(s) 2578
BsaI GGTCTC 3 cut(s) 52, 2050, 2052
BsaJI CCNNGG 1 cut(s) 2023
Bsc4I CCNNNNNNNGG 2 cut(s) 2207, 2213
Bse118I RCCGGY 1 cut(s) 416
Bse1I ACTGG 9 cut(s) 82, 1011, 1468, 1524, 1991, 2091, 2111, 2242, 2540
Bse3DI GCAATG 4 cut(s) 1290, 1563, 2560, 2801
BseBI CCWGG 7 cut(s) 680, 1664, 2025, 2129, 2208, 2565, 2715
BseDI CCNNGG 1 cut(s) 2023
BseGI GGATG 6 cut(s) 53, 342, 632, 997, 1007, 1951
BseLI CCNNNNNNNGG 2 cut(s) 2207, 2213
BseMI GCAATG 4 cut(s) 1290, 1563, 2560, 2801
BseMII CTCAG 5 cut(s) 855, 915, 1644, 2056, 2333
BseNI ACTGG 9 cut(s) 82, 1011, 1468, 1524, 1991, 2091, 2111, 2242, 2540
BseRI GAGGAG 2 cut(s) 863, 1106
BseXI GCAGC 7 cut(s) 156, 346, 618, 1274, 2142, 2263, 2789
BsgI GTGCAG 2 cut(s) 2047, 2250
BsiHKAI GWGCWC 2 cut(s) 1280, 1350
BsiHKCI CYCGRG 2 cut(s) 1343, 1349
BsiSI CCGG 3 cut(s) 41, 417, 535
BslFI GGGAC 1 cut(s) 719
BslI CCNNNNNNNGG 2 cut(s) 2207, 2213
BsmAI GTCTC 9 cut(s) 52, 1786, 2009, 2050, 2052, 2129, 2322, 2750, 2763
BsmBI CGTCTC 2 cut(s) 1786, 2129
BsmFI GGGAC 1 cut(s) 719
Bso31I GGTCTC 3 cut(s) 52, 2050, 2052
BsoBI CYCGRG 2 cut(s) 1343, 1349
Bsp119I TTCGAA 2 cut(s) 65, 2518
Bsp1286I GDGCHC 2 cut(s) 1280, 1350
Bsp1407I TGTACA 1 cut(s) 1860
Bsp143I GATC 8 cut(s) 892, 1033, 1732, 2038, 2119, 2262, 2717, 2807
Bsp1720I GCTNAGC 1 cut(s) 363
BspACI CCGC 5 cut(s) 244, 2162, 2371, 2392, 2560
BspCNI CTCAG 5 cut(s) 854, 916, 1645, 2055, 2332
BspHI TCATGA 1 cut(s) 1036
BspLI GGNNCC 4 cut(s) 90, 1132, 1625, 1852
BspMAI CTGCAG 1 cut(s) 2779
BspMI ACCTGC 2 cut(s) 19, 816
BspOI GCTAGC 1 cut(s) 1643
BspPI GGATC 2 cut(s) 2127, 2725
BspT104I TTCGAA 2 cut(s) 65, 2518
BspTI CTTAAG 1 cut(s) 2134
BspTNI GGTCTC 3 cut(s) 52, 2050, 2052
BsrBI CCGCTC 1 cut(s) 244
BsrDI GCAATG 4 cut(s) 1290, 1563, 2560, 2801
BsrFI RCCGGY 1 cut(s) 416
BsrGI TGTACA 1 cut(s) 1860
BsrI ACTGG 9 cut(s) 82, 1011, 1468, 1524, 1991, 2091, 2111, 2242, 2540
BssAI RCCGGY 1 cut(s) 416
BssECI CCNNGG 1 cut(s) 2023
BssMI GATC 8 cut(s) 892, 1033, 1732, 2038, 2119, 2262, 2717, 2807
Bst2UI CCWGG 7 cut(s) 680, 1664, 2025, 2129, 2208, 2565, 2715
Bst4CI ACNGT 5 cut(s) 402, 565, 1681, 1876, 1910
Bst6I CTCTTC 5 cut(s) 287, 840, 1148, 2048, 2354
BstAFI CTTAAG 1 cut(s) 2134
BstAPI GCANNNNNTGC 1 cut(s) 1649
BstAUI TGTACA 1 cut(s) 1860
BstBAI YACGTR 1 cut(s) 2578
BstBI TTCGAA 2 cut(s) 65, 2518
BstDEI CTNAG 9 cut(s) 363, 841, 924, 1304, 1653, 1736, 2042, 2319, 2450
BstF5I GGATG 6 cut(s) 53, 342, 632, 997, 1007, 1951
BstHHI GCGC 1 cut(s) 1408
BstKTI GATC 8 cut(s) 895, 1036, 1735, 2041, 2122, 2265, 2720, 2810
BstMAI GTCTC 9 cut(s) 52, 1786, 2009, 2050, 2052, 2129, 2322, 2750, 2763
BstMBI GATC 8 cut(s) 892, 1033, 1732, 2038, 2119, 2262, 2717, 2807
BstMWI GCNNNNNNNGC 7 cut(s) 510, 696, 1649, 1886, 1895, 2161, 2638
BstNI CCWGG 7 cut(s) 680, 1664, 2025, 2129, 2208, 2565, 2715
BstNSI RCATGY 8 cut(s) 191, 659, 690, 1235, 1589, 1639, 1647, 2229
BstSCI CCNGG 8 cut(s) 534, 678, 1662, 2023, 2127, 2206, 2563, 2713
BstSFI CTRYAG 2 cut(s) 2482, 2775
BstV1I GCAGC 7 cut(s) 156, 346, 618, 1274, 2142, 2263, 2789
BstV2I GAAGAC 1 cut(s) 1522
BstX2I RGATCY 2 cut(s) 892, 2038
BstYI RGATCY 2 cut(s) 892, 2038
BsuI GTATCC 1 cut(s) 2740
BtgZI GCGATG 2 cut(s) 1199, 1468
BtrI CACGTC 1 cut(s) 733
BtsCI GGATG 6 cut(s) 53, 342, 632, 997, 1007, 1951
BtsI GCAGTG 1 cut(s) 1278
BtsIMutI CAGTG 2 cut(s) 1278, 2118
BveI ACCTGC 2 cut(s) 19, 816
CaiI CAGNNNCTG 1 cut(s) 2237
CciI TCATGA 1 cut(s) 1036
CfoI GCGC 1 cut(s) 1408
Cfr10I RCCGGY 1 cut(s) 416
Cfr13I GGNCC 7 cut(s) 88, 419, 643, 805, 1131, 1308, 2645
CseI GACGC 2 cut(s) 163, 2559
CsiI ACCWGGT 1 cut(s) 678
Csp6I GTAC 6 cut(s) 496, 517, 653, 1703, 1861, 2819
CviQI GTAC 6 cut(s) 496, 517, 653, 1703, 1861, 2819
DdeI CTNAG 9 cut(s) 363, 841, 924, 1304, 1653, 1736, 2042, 2319, 2450
DpnI GATC 8 cut(s) 894, 1035, 1734, 2040, 2121, 2264, 2719, 2809
DpnII GATC 8 cut(s) 892, 1033, 1732, 2038, 2119, 2262, 2717, 2807
Eam1104I CTCTTC 5 cut(s) 287, 840, 1148, 2048, 2354
EarI CTCTTC 5 cut(s) 287, 840, 1148, 2048, 2354
Ecl136II GAGCTC 1 cut(s) 1348
Eco147I AGGCCT 1 cut(s) 1885
Eco24I GRGCYC 1 cut(s) 1350
Eco31I GGTCTC 3 cut(s) 52, 2050, 2052
Eco32I GATATC 2 cut(s) 888, 2584
Eco47I GGWCC 4 cut(s) 88, 805, 1131, 1308
Eco53kI GAGCTC 1 cut(s) 1348
Eco57I CTGAAG 3 cut(s) 252, 730, 2703
Eco88I CYCGRG 2 cut(s) 1343, 1349
EcoICRI GAGCTC 1 cut(s) 1348
EcoO109I RGGNCCY 2 cut(s) 1131, 1308
EcoRI GAATTC 2 cut(s) 67, 539
EcoRII CCWGG 7 cut(s) 678, 1662, 2023, 2127, 2206, 2563, 2713
EcoRV GATATC 2 cut(s) 888, 2584
EcoT22I ATGCAT 1 cut(s) 1637
EcoT38I GRGCYC 1 cut(s) 1350
Esp3I CGTCTC 2 cut(s) 1786, 2129
FalI AAGNNNNNCTT 4 cut(s) 1426, 1458, 1806, 1838
FaqI GGGAC 1 cut(s) 719
Fnu4HI GCNGC 8 cut(s) 170, 360, 632, 1263, 2156, 2277, 2561, 2778
FokI GGATG 6 cut(s) 40, 329, 639, 994, 1004, 1958
FriOI GRGCYC 1 cut(s) 1350
FseI GGCCGGCC 1 cut(s) 420
Fsp4HI GCNGC 8 cut(s) 170, 360, 632, 1263, 2156, 2277, 2561, 2778
FspBI CTAG 6 cut(s) 1025, 1067, 1640, 1706, 1881, 2547
GlaI GCGC 1 cut(s) 1407
GluI GCNGC 8 cut(s) 170, 360, 632, 1263, 2156, 2277, 2561, 2778
GsuI CTGGAG 6 cut(s) 99, 1206, 1974, 2094, 2108, 2259
HapII CCGG 3 cut(s) 41, 417, 535
HgaI GACGC 2 cut(s) 163, 2559
HhaI GCGC 1 cut(s) 1408
Hin6I GCGC 1 cut(s) 1406
HinP1I GCGC 1 cut(s) 1406
HincII GTYRAC 2 cut(s) 579, 2533
HindII GTYRAC 2 cut(s) 579, 2533
HindIII AAGCTT 1 cut(s) 1532
HinfI GANTC 6 cut(s) 4, 333, 759, 767, 1166, 1772
HpaII CCGG 3 cut(s) 41, 417, 535
HphI GGTGA 7 cut(s) 286, 694, 762, 1073, 1332, 1756, 2712
Hpy166II GTNNAC 6 cut(s) 8, 579, 805, 1064, 1202, 2533
Hpy188III TCNNGA 8 cut(s) 72, 547, 989, 1025, 1037, 1125, 2623, 2681
Hpy8I GTNNAC 6 cut(s) 8, 579, 805, 1064, 1202, 2533
HpyAV CCTTC 7 cut(s) 656, 974, 1008, 1840, 1900, 2110, 2625
HpyCH4III ACNGT 5 cut(s) 402, 565, 1681, 1876, 1910
HpyCH4IV ACGT 4 cut(s) 732, 775, 1380, 2577
HpyF10VI GCNNNNNNNGC 7 cut(s) 510, 696, 1649, 1886, 1895, 2161, 2638
HpyF3I CTNAG 9 cut(s) 363, 841, 924, 1304, 1653, 1736, 2042, 2319, 2450
HpySE526I ACGT 4 cut(s) 732, 775, 1380, 2577
HspAI GCGC 1 cut(s) 1406
KroI GCCGGC 1 cut(s) 416
KroNI GCCGGC 1 cut(s) 418
Kzo9I GATC 8 cut(s) 892, 1033, 1732, 2038, 2119, 2262, 2717, 2807
LmnI GCTCC 5 cut(s) 391, 593, 1116, 1450, 2089
Lsp1109I GCAGC 7 cut(s) 156, 346, 618, 1274, 2142, 2263, 2789
LweI GCATC 9 cut(s) 62, 547, 824, 929, 1033, 1373, 1622, 1936, 2692
MabI ACCWGGT 1 cut(s) 678
MaeI CTAG 6 cut(s) 1025, 1067, 1640, 1706, 1881, 2547
MaeII ACGT 4 cut(s) 732, 775, 1380, 2577
MaeIII GTNAC 5 cut(s) 682, 1459, 1681, 2138, 2653
MalI GATC 8 cut(s) 894, 1035, 1734, 2040, 2121, 2264, 2719, 2809
MbiI CCGCTC 1 cut(s) 244
MboI GATC 8 cut(s) 892, 1033, 1732, 2038, 2119, 2262, 2717, 2807
MfeI CAATTG 1 cut(s) 2312
MflI RGATCY 2 cut(s) 892, 2038
MhlI GDGCHC 2 cut(s) 1280, 1350
MlyI GAGTC 1 cut(s) 13
MmeI TCCRAC 4 cut(s) 81, 117, 2216, 2377
Mph1103I ATGCAT 1 cut(s) 1637
MroNI GCCGGC 1 cut(s) 416
MroXI GAANNNNTTC 1 cut(s) 984
MslI CAYNNNNRTG 4 cut(s) 925, 1041, 1236, 1283
MspA1I CMGCKG 2 cut(s) 2371, 2651
MspCI CTTAAG 1 cut(s) 2134
MspI CCGG 3 cut(s) 41, 417, 535
MspR9I CCNGG 8 cut(s) 536, 680, 1664, 2025, 2129, 2208, 2565, 2715
MunI CAATTG 1 cut(s) 2312
MvaI CCWGG 7 cut(s) 680, 1664, 2025, 2129, 2208, 2565, 2715
MwoI GCNNNNNNNGC 7 cut(s) 510, 696, 1649, 1886, 1895, 2161, 2638
NaeI GCCGGC 1 cut(s) 418
NciI CCSGG 1 cut(s) 536
NdeII GATC 8 cut(s) 892, 1033, 1732, 2038, 2119, 2262, 2717, 2807
NgoMIV GCCGGC 1 cut(s) 416
NheI GCTAGC 1 cut(s) 1639
NlaIV GGNNCC 4 cut(s) 90, 1132, 1625, 1852
NmeAIII GCCGAG 1 cut(s) 1874
NmuCI GTSAC 2 cut(s) 682, 1681
NsiI ATGCAT 1 cut(s) 1637
NspI RCATGY 8 cut(s) 191, 659, 690, 1235, 1589, 1639, 1647, 2229
NspV TTCGAA 2 cut(s) 65, 2518
PaeI GCATGC 3 cut(s) 1589, 1639, 1647
PaeR7I CTCGAG 1 cut(s) 1349
PagI TCATGA 1 cut(s) 1036
PaqCI CACCTGC 1 cut(s) 19
PceI AGGCCT 1 cut(s) 1885
PciI ACATGT 3 cut(s) 187, 655, 2225
PdiI GCCGGC 1 cut(s) 418
PdmI GAANNNNTTC 1 cut(s) 984
PfeI GAWTC 5 cut(s) 333, 759, 767, 1166, 1772
PflMI CCANNNNNTGG 1 cut(s) 2213
PfoI TCCNGGA 2 cut(s) 2206, 2713
PkrI GCNGC 8 cut(s) 171, 361, 633, 1264, 2157, 2278, 2562, 2779
PleI GAGTC 1 cut(s) 12
PpsI GAGTC 1 cut(s) 12
Ppu21I YACGTR 1 cut(s) 2578
PpuMI RGGWCCY 2 cut(s) 1131, 1308
PscI ACATGT 3 cut(s) 187, 655, 2225
PshBI ATTAAT 1 cut(s) 2289
PsiI TTATAA 1 cut(s) 1085
Psp124BI GAGCTC 1 cut(s) 1350
Psp1406I AACGTT 1 cut(s) 1380
Psp5II RGGWCCY 2 cut(s) 1131, 1308
Psp6I CCWGG 7 cut(s) 678, 1662, 2023, 2127, 2206, 2563, 2713
PspGI CCWGG 7 cut(s) 678, 1662, 2023, 2127, 2206, 2563, 2713
PspN4I GGNNCC 4 cut(s) 90, 1132, 1625, 1852
PspPI GGNCC 7 cut(s) 88, 419, 643, 805, 1131, 1308, 2645
PspPPI RGGWCCY 2 cut(s) 1131, 1308
PspXI VCTCGAGB 1 cut(s) 1349
PstI CTGCAG 1 cut(s) 2779
PstNI CAGNNNCTG 1 cut(s) 2237
PsuI RGATCY 2 cut(s) 892, 2038
PvuII CAGCTG 1 cut(s) 2651
RigI GGCCGGCC 1 cut(s) 420
RsaI GTAC 6 cut(s) 497, 518, 654, 1704, 1862, 2820
RsaNI GTAC 6 cut(s) 496, 517, 653, 1703, 1861, 2819
RseI CAYNNNNRTG 4 cut(s) 925, 1041, 1236, 1283
SacI GAGCTC 1 cut(s) 1350
SatI GCNGC 8 cut(s) 170, 360, 632, 1263, 2156, 2277, 2561, 2778
Sau3AI GATC 8 cut(s) 892, 1033, 1732, 2038, 2119, 2262, 2717, 2807
Sau96I GGNCC 7 cut(s) 88, 419, 643, 805, 1131, 1308, 2645
ScaI AGTACT 1 cut(s) 518
SchI GAGTC 1 cut(s) 13
ScrFI CCNGG 8 cut(s) 536, 680, 1664, 2025, 2129, 2208, 2565, 2715
SduI GDGCHC 2 cut(s) 1280, 1350
SexAI ACCWGGT 1 cut(s) 678
SfaNI GCATC 9 cut(s) 62, 547, 824, 929, 1033, 1373, 1622, 1936, 2692
SfcI CTRYAG 2 cut(s) 2482, 2775
Sfr274I CTCGAG 1 cut(s) 1349
SfuI TTCGAA 2 cut(s) 65, 2518
SinI GGWCC 4 cut(s) 88, 805, 1131, 1308
SlaI CTCGAG 1 cut(s) 1349
SmiMI CAYNNNNRTG 4 cut(s) 925, 1041, 1236, 1283
SmlI CTYRAG 7 cut(s) 464, 499, 1349, 1418, 1529, 1575, 2134
SmoI CTYRAG 7 cut(s) 464, 499, 1349, 1418, 1529, 1575, 2134
SphI GCATGC 3 cut(s) 1589, 1639, 1647
SseBI AGGCCT 1 cut(s) 1885
SsiI CCGC 5 cut(s) 244, 2162, 2371, 2392, 2560
SspI AATATT 1 cut(s) 952
SspMI CTAG 6 cut(s) 1025, 1067, 1640, 1706, 1881, 2547
SstI GAGCTC 1 cut(s) 1350
StuI AGGCCT 1 cut(s) 1885
StyD4I CCNGG 8 cut(s) 534, 678, 1662, 2023, 2127, 2206, 2563, 2713
TaaI ACNGT 5 cut(s) 402, 565, 1681, 1876, 1910
TaiI ACGT 4 cut(s) 735, 778, 1383, 2580
TaqI TCGA 5 cut(s) 65, 103, 1350, 2301, 2518
TatI WGTACW 3 cut(s) 495, 516, 1860
TauI GCSGC 1 cut(s) 2563
TfiI GAWTC 5 cut(s) 333, 759, 767, 1166, 1772
TscAI CASTG 2 cut(s) 1285, 2118
TseFI GTSAC 2 cut(s) 682, 1681
TseI GCWGC 7 cut(s) 169, 359, 631, 1262, 2155, 2276, 2777
Tsp45I GTSAC 2 cut(s) 682, 1681
TspDTI ATGAA 7 cut(s) 951, 1008, 1747, 1782, 1797, 2599, 2616
TspGWI ACGGA 1 cut(s) 141
TspRI CASTG 2 cut(s) 1285, 2118
Van91I CCANNNNNTGG 1 cut(s) 2213
Vha464I CTTAAG 1 cut(s) 2134
VpaK11BI GGWCC 4 cut(s) 88, 805, 1131, 1308
VspI ATTAAT 1 cut(s) 2289
XapI RAATTY 7 cut(s) 67, 202, 348, 539, 1138, 1804, 2095
XbaI TCTAGA 1 cut(s) 1024
XceI RCATGY 8 cut(s) 191, 659, 690, 1235, 1589, 1639, 1647, 2229
XhoI CTCGAG 1 cut(s) 1349
XmnI GAANNNNTTC 1 cut(s) 984
XspI CTAG 6 cut(s) 1025, 1067, 1640, 1706, 1881, 2547
ZrmI AGTACT 1 cut(s) 518
Zsp2I ATGCAT 1 cut(s) 1637
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.