Prupe.8G086800_v2.0.a1

Retinoblastoma-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0003674
Physical Location & Seq
Reverse (-)
11963535 .. 11974663
11129 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G086800.3

Sequence Viewer

Length: 3048 bp
ATGAGTCCACCTGCTTTGGTGAAGATGGAAGATACTAACCCTAAAGTTTTAGCTTCCAAGAATTCAAATTCCGAAAGCAGAGATTCAGACCCCATCCAAGCTCGATTTAATGATTTGTGCAAGAATGGATTGTCGTTGGATGATAATGCTTACACGCAAGCTATGAAGCTGTTCAAAGAAACCAAACATCTATTAACTTCAAATGCATTGGCTATTGGAAACGGAACACCTGAAGAAGCAGAACAGTTCTGGTTTGCCTTTGTTTTGTACTCCGTGAAAACTTTGAGTGAGAATAATTCACAGATGAGCTCTGATGATAATGGATTTTCTCTCTTCCAAATTTTGAGAGCTGCGAAGATGAACATTGTAGATTTTTTCAAGGAGCTTCCTCAGTTAGTTGTTAAGGCCGGTCCAGTTTTGAGTGATCTTTATGGCATAGATTGGGAAAACAAACTTGAGGCAAAAGAGTTGCAGGCAAATTTTGAGTACTTGAGCCTGTTATGCAAGTACTACAAACGTGTATACCGAGAGGTCTTCTTGACAAGTGATGTAAATGCCGATAAGCAGTCAGCTGTTGCCAGAGGAACTGGTTATGTGTCGGAGTATCATCGTTTTGGATGGTTGCTGTTTTTGGCCCTTCGTGTACATGTATTCAGCCGATTTAAAGACCTGTTGACTTGTGCAAATGGCTTGGTTGCCATATTGGCAATTTTAATTATTCATGTTCCTGTTTGCTTCAGAAAATTCAACATCCATGATTCAACACACTTTGTGAAAGGCAGCAAAGGCGTGGACCTGCTTGCGTCCCTTTGCAACATTTATGACACTTCAGAAGATGAGTTGAGAAAAACAATGGAAATGGCTAATGCTTTAATAGCGGACATTTTGAAGAAGAAGCCCTGTTCAGCGTCTGAGTGTAGAAATAAAAATCTAGAGAATATGGATCCAGATGGTTTGACCTACTTTGAAGGTCTTATGGAGGAACCATCATTGTCATCCAGTTTAGATATTCTAGAAAAGGATTACGATGATGCAATTCGGAGTAAGGGTGAACTTGATGAGAGAGTCTTCATCAACGAAGAAGATAGCTTACTTGGTTCTGGTAGCTTTTCTGGGGGATCCATGAATTTATCCGGTGTGAAGAGGAAATTTGATTCTATAGCCTCCCCAACAAAGCCAATTGCAAGTCCACTGTCTCCCCATCACACTCCTGCATCTCATGTTAATGGTGGTGCTAATTCAAAGATGGTAGCCACACCAGTGAGCACTGCAATGACAACTGCAAAGTGGCTTCGGACCTTTATTTCTCCACTTCCGTCAAAACCCTCAGCCAAGCTAGAGGGGTTCCTGGCATTGTGTGATAGGGATGTTACCAATGATGTGGTACGCAGGGCACAAGTCATATTGGAGGTTATATTTCCAAGTAGTGCCTTGGGAGAACGATTTGTAACTGGTAGCCTGCAAAGTGCTGACCTCATGGACAACATCTGGGCAGAACAACGAAGATTGGAAGCACTCAAACTTTATTATAGAGTTTTGGAGGCAATCTGTAGGGCAGAGGCTCAAATACTGCATGCTACTAATTTGACCTCTTTATTAACCAATGAGAGGTTCCATAGATGCATGCTAGCCTGCTCTGCTGAGTTAGTCCTGGCAACACATAAGACTGTCACAATGTTGTTTCCAGCAGTGTTGGAGAGGACAGGTATTACAGCTTTTGATCTTAGCAAGGTGATAGAGAGCTTCATTAGACATGAGGAATCTCTTCCAAGAGAATTAAGACGACACTTGAATTCATTGGAAGAACGACTTTTGGAGAGCATGGTATGGGAAAAGGGTTCCTCGATGTATAATTCTTTGACTGTTGCAAGACCTGCCCTCTCAGCAGAAATAAATCGTCTTGAGTTATTAGCAGAACCCATGCCATCTTTGGATGCAATCGCCATGCGTATTACCTTCTCTTGTGGAGGGTTGCCTCTGGCACCTTCTTTACAGAAGCATGAGAATTCACCAGGTCAGAATGGAGATATCAGGTCTCCAAAGATGGAGCAGAATTCCTTTACATCTCCTGTGAAAGATCGTTTTCAGGCCTTAAGTAACCTAAAGTCAAATCTGCCAGCCCCCCCTTTACAGTCTGCATTTGCCAGTCCAACACGGCCAAATCCGGGAGGTGGAGGGGAAACATGTGCAGAAACTGGAATTAGTATTTTCTTTAGCAAGATAATCAAGCTGGCGGCTGTCAGAATCAATGGTATGGTTGAAAGGATGCAACTGTCTCAGCAGATCAGAGAGAATGTTTATCGTCTCTTTCAACAAATCCTTGTTCAACGGACATCTCTCTTTTTTAACCGCCACATCGACCAGATTATTCTTTGTTGTTTTTATGGAGTTGCAAAGATTTCTCAACTTAATTTAACATTTAGGCAAATCTTATACAACTATACAAAGCAACCACAATATAAACCGCAAGTTTTTCGAAGTGTGTTTGTTAATTGGCCTTCAGCACGCCGCAATGGGAGGCCAGCACAGGAACACATTGAAATCATACCATTTTACAATGAAATATTCATTCCTTCTGTGAAATCTCTGCTAGTCGAGGTTGGACCAGGTCCAACAACTACAAGAGATAGCATAGTTCCTGAAGCAAACAATGACAATGATGCTCAATGTCCTGGATCACCTAAAGTATCCAGCTTTCCAAGTCTCCCTGATATGTCTCCAAAGAAAGTATCTGCAGCACACAATGTTTATGTCTCTCCATTGCGATCATTTAAGATGGATGCTTTGATCTCGCATAGCACAAAAAGCTATTATGCTTGTGTTGGGGAGTGTACCGTTGCGTATCAGAGCCCTTCGAAAGATCTCACTGCCATCAACAACCGCTTGAATGGTACGCGAAAGCTCAGAGGCACTCTCAACTTTGACAATGTTGACGTAGGCTTGGTCAGTGATTCTATGGTGGTCAACAGCCTCTACCTTCAGAATCAAAATGGAAGTTGTGCATCCTCATCAGGTGCATCGGTGAAAACTGAGCAACAGGACTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000082 GO:0000278 GO:0000280 GO:0000902 GO:0000904 GO:0001558 GO:0001708 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006275 GO:0006349 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007059 GO:0007127 GO:0007129 GO:0007275 GO:0008134 GO:0008150 GO:0008152 GO:0008356 GO:0009553 GO:0009555 GO:0009566 GO:0009567 GO:0009653 GO:0009791 GO:0009888 GO:0009889 GO:0009987 GO:0010016 GO:0010026 GO:0010052 GO:0010090 GO:0010103 GO:0010374 GO:0010377 GO:0010468 GO:0010556 GO:0010564 GO:0016043 GO:0019219 GO:0019222 GO:0019953 GO:0022402 GO:0022414 GO:0022619 GO:0030154 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032875 GO:0032989 GO:0033043 GO:0034641 GO:0040008 GO:0040029 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0044770 GO:0044772 GO:0044843 GO:0045132 GO:0045143 GO:0045165 GO:0046483 GO:0048229 GO:0048285 GO:0048366 GO:0048367 GO:0048468 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051052 GO:0051128 GO:0051171 GO:0051239 GO:0051276 GO:0051301 GO:0051321 GO:0051704 GO:0051726 GO:0051783 GO:0055046 GO:0060255 GO:0061982 GO:0065007 GO:0070192 GO:0071514 GO:0071704 GO:0071840 GO:0080090 GO:0090329 GO:0090558 GO:0090626 GO:0090627 GO:0090698 GO:0097159 GO:0098813 GO:0099402 GO:0140013 GO:1901360 GO:1901363 GO:1903046 GO:1903047 GO:1903866 GO:2000036 GO:2000112 GO:2000653
KEGG Pathways
Metabolic & Signaling
ko04110 ko04218 ko04350 ko05165 ko05203 map04110 map04218 map04350 map05165 map05203
Pfam Domains
Protein Families
DUF3452 RB_A RB_B Rb_C

Protein Analysis

1016

Amino Acids

112.87

Weight (kDa)

7.51

Isoelectric Point (pI)

50.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 19
Acc36I ACCTGC 3 cut(s) 19, 804, 1882
AccB1I GGYRCC 1 cut(s) 1981
AccI GTMKAC 1 cut(s) 522
AccII CGCG 1 cut(s) 2896
AciI CCGC 6 cut(s) 878, 2234, 2350, 2465, 2509, 2881
AclWI GGATC 5 cut(s) 938, 951, 1113, 1126, 2683
AcoI YGGCCR 1 cut(s) 2156
AcuI CTGAAG 6 cut(s) 252, 721, 813, 2484, 2661, 2963
AdeI CACNNNGTG 1 cut(s) 772
AfaI GTAC 7 cut(s) 269, 488, 509, 645, 1386, 2833, 2893
AfiI CCNNNNNNNGG 3 cut(s) 1608, 2165, 2171
AflII CTTAAG 1 cut(s) 2092
AflIII ACRYGT 3 cut(s) 517, 646, 2183
AjnI CCWGG 5 cut(s) 1347, 1650, 2011, 2605, 2671
AjuI GAANNNNNNNTTGG 2 cut(s) 1595, 1627
AleI CACNNNNGTG 1 cut(s) 1259
Alw21I GWGCWC 2 cut(s) 311, 1268
Alw26I GTCTC 7 cut(s) 1200, 2040, 2280, 2309, 2708, 2721, 2758
AlwI GGATC 5 cut(s) 938, 951, 1113, 1126, 2683
AlwNI CAGNNNCTG 2 cut(s) 911, 2195
AoxI GGCC 6 cut(s) 405, 633, 2088, 2156, 2495, 2519
ApeKI GCWGC 3 cut(s) 350, 780, 2735
Asp700I GAANNNNTTC 2 cut(s) 170, 1764
AspS9I GGNCC 6 cut(s) 410, 634, 793, 1296, 2603, 2609
AsuC2I CCSGG 1 cut(s) 2166
AsuHPI GGTGA 6 cut(s) 31, 1061, 1744, 2001, 2670, 3034
AsuII TTCGAA 2 cut(s) 2476, 2855
AsuNHI GCTAGC 1 cut(s) 1627
AvaII GGWCC 5 cut(s) 410, 793, 1296, 2603, 2609
BaeGI GKGCMC 1 cut(s) 1396
BamHI GGATCC 2 cut(s) 943, 1118
BanI GGYRCC 1 cut(s) 1981
BanII GRGCYC 2 cut(s) 311, 2852
BarI GAAGNNNNNNTAC 2 cut(s) 1074, 1106
BbsI GAAGAC 2 cut(s) 526, 1060
Bbv12I GWGCWC 2 cut(s) 311, 1268
BbvCI CCTCAGC 1 cut(s) 1327
BbvI GCAGC 3 cut(s) 337, 792, 2747
BceAI ACGGC 1 cut(s) 2171
BcgI CGANNNNNNTGC 2 cut(s) 2456, 2490
BciT130I CCWGG 5 cut(s) 1349, 1652, 2013, 2607, 2673
BciVI GTATCC 1 cut(s) 2698
BcnI CCSGG 1 cut(s) 2166
BcoDI GTCTC 7 cut(s) 1200, 2040, 2280, 2309, 2708, 2721, 2758
BfaI CTAG 5 cut(s) 932, 1013, 1337, 1628, 2591
BfmI CTRYAG 3 cut(s) 1158, 1549, 2733
BfrI CTTAAG 1 cut(s) 2092
BfuAI ACCTGC 3 cut(s) 19, 804, 1882
BfuI GTATCC 1 cut(s) 2698
BglI GCCNNNNNGGC 1 cut(s) 704
BglII AGATCT 1 cut(s) 2860
BisI GCNGC 5 cut(s) 351, 781, 2235, 2509, 2736
BlsI GCNGC 5 cut(s) 352, 782, 2236, 2510, 2737
BmcAI AGTACT 2 cut(s) 488, 509
Bme1390I CCNGG 6 cut(s) 1349, 1652, 2013, 2166, 2607, 2673
Bme18I GGWCC 5 cut(s) 410, 793, 1296, 2603, 2609
BmgT120I GGNCC 6 cut(s) 410, 634, 793, 1296, 2603, 2609
BmiI GGNNCC 7 cut(s) 945, 984, 1120, 1346, 1613, 1840, 1983
BmrFI CCNGG 6 cut(s) 1349, 1652, 2013, 2166, 2607, 2673
BmsI GCATC 9 cut(s) 1021, 1223, 1610, 1924, 2256, 2650, 2770, 3011, 3026
BmtI GCTAGC 1 cut(s) 1631
BpiI GAAGAC 2 cut(s) 526, 1060
BplI GAGNNNNNCTC 2 cut(s) 2898, 2930
Bpu10I CCTNAGC 1 cut(s) 1327
Bpu14I TTCGAA 2 cut(s) 2476, 2855
BpuEI CTTGAG 3 cut(s) 476, 511, 1922
BpuMI CCSGG 1 cut(s) 2166
BsaI GGTCTC 1 cut(s) 2040
BsaJI CCNNGG 1 cut(s) 1431
BsaWI WCCGGW 1 cut(s) 1133
BsaXI ACNNNNNCTCC 2 cut(s) 1033, 1063
Bsc4I CCNNNNNNNGG 3 cut(s) 1608, 2165, 2171
Bse118I RCCGGY 1 cut(s) 407
Bse1I ACTGG 7 cut(s) 413, 592, 999, 1259, 1456, 2145, 2200
Bse3DI GCAATG 3 cut(s) 1278, 2518, 2759
BseBI CCWGG 5 cut(s) 1349, 1652, 2013, 2607, 2673
BseDI CCNNGG 1 cut(s) 1431
BseLI CCNNNNNNNGG 3 cut(s) 1608, 2165, 2171
BseMI GCAATG 3 cut(s) 1278, 2518, 2759
BseMII CTCAG 8 cut(s) 404, 903, 1341, 1632, 1896, 2291, 2917, 3021
BseNI ACTGG 7 cut(s) 413, 592, 999, 1259, 1456, 2145, 2200
BseSI GKGCMC 1 cut(s) 1396
BseXI GCAGC 3 cut(s) 337, 792, 2747
BsgI GTGCAG 1 cut(s) 2208
Bsh1236I CGCG 1 cut(s) 2896
BshFI GGCC 6 cut(s) 407, 635, 2090, 2158, 2497, 2521
BshNI GGYRCC 1 cut(s) 1981
BsiHKAI GWGCWC 2 cut(s) 311, 1268
BsiSI CCGG 3 cut(s) 408, 1134, 2165
BslFI GGGAC 1 cut(s) 790
BslI CCNNNNNNNGG 3 cut(s) 1608, 2165, 2171
BsmAI GTCTC 7 cut(s) 1200, 2040, 2280, 2309, 2708, 2721, 2758
BsmBI CGTCTC 1 cut(s) 2309
BsmFI GGGAC 1 cut(s) 790
BsnI GGCC 6 cut(s) 407, 635, 2090, 2158, 2497, 2521
Bso31I GGTCTC 1 cut(s) 2040
Bsp119I TTCGAA 2 cut(s) 2476, 2855
Bsp1286I GDGCHC 4 cut(s) 311, 1268, 1396, 2852
Bsp1407I TGTACA 1 cut(s) 643
BspACI CCGC 6 cut(s) 878, 2234, 2350, 2465, 2509, 2881
BspANI GGCC 6 cut(s) 407, 635, 2090, 2158, 2497, 2521
BspCNI CTCAG 8 cut(s) 403, 904, 1340, 1633, 1895, 2290, 2916, 3022
BspFNI CGCG 1 cut(s) 2896
BspLI GGNNCC 7 cut(s) 945, 984, 1120, 1346, 1613, 1840, 1983
BspMAI CTGCAG 1 cut(s) 2737
BspMI ACCTGC 3 cut(s) 19, 804, 1882
BspOI GCTAGC 1 cut(s) 1631
BspPI GGATC 5 cut(s) 938, 951, 1113, 1126, 2683
BspT104I TTCGAA 2 cut(s) 2476, 2855
BspT107I GGYRCC 1 cut(s) 1981
BspTI CTTAAG 1 cut(s) 2092
BspTNI GGTCTC 1 cut(s) 2040
BsrDI GCAATG 3 cut(s) 1278, 2518, 2759
BsrFI RCCGGY 1 cut(s) 407
BsrGI TGTACA 1 cut(s) 643
BsrI ACTGG 7 cut(s) 413, 592, 999, 1259, 1456, 2145, 2200
BssAI RCCGGY 1 cut(s) 407
BssECI CCNNGG 1 cut(s) 1431
BssNAI GTATAC 1 cut(s) 523
BssT1I CCWWGG 1 cut(s) 1431
Bst1107I GTATAC 1 cut(s) 523
Bst2UI CCWGG 5 cut(s) 1349, 1652, 2013, 2607, 2673
Bst4CI ACNGT 7 cut(s) 246, 1194, 1669, 1864, 2133, 2274, 2836
Bst6I CTCTTC 3 cut(s) 338, 1136, 1770
BstAFI CTTAAG 1 cut(s) 2092
BstAPI GCANNNNNTGC 1 cut(s) 1874
BstAUI TGTACA 1 cut(s) 643
BstBI TTCGAA 2 cut(s) 2476, 2855
BstDEI CTNAG 9 cut(s) 390, 912, 1327, 1641, 1724, 1882, 2277, 2903, 3030
BstFNI CGCG 1 cut(s) 2896
BstMAI GTCTC 7 cut(s) 1200, 2040, 2280, 2309, 2708, 2721, 2758
BstMWI GCNNNNNNNGC 8 cut(s) 501, 704, 786, 875, 1637, 1874, 1883, 2805
BstNI CCWGG 5 cut(s) 1349, 1652, 2013, 2607, 2673
BstNSI RCATGY 4 cut(s) 650, 1577, 1627, 2187
BstSCI CCNGG 6 cut(s) 1347, 1650, 2011, 2164, 2605, 2671
BstSFI CTRYAG 3 cut(s) 1158, 1549, 2733
BstSLI GKGCMC 1 cut(s) 1396
BstUI CGCG 1 cut(s) 2896
BstV1I GCAGC 3 cut(s) 337, 792, 2747
BstV2I GAAGAC 2 cut(s) 526, 1060
BstX2I RGATCY 3 cut(s) 943, 1118, 2860
BstXI CCANNNNNNTGG 1 cut(s) 1381
BstYI RGATCY 3 cut(s) 943, 1118, 2860
BstZ17I GTATAC 1 cut(s) 523
BsuI GTATCC 1 cut(s) 2698
BsuRI GGCC 6 cut(s) 407, 635, 2090, 2158, 2497, 2521
BtsI GCAGTG 3 cut(s) 1266, 1695, 2865
BtsIMutI CAGTG 6 cut(s) 1190, 1266, 1266, 1695, 2865, 2953
BveI ACCTGC 3 cut(s) 19, 804, 1882
CaiI CAGNNNCTG 2 cut(s) 911, 2195
Cfr10I RCCGGY 1 cut(s) 407
Cfr13I GGNCC 6 cut(s) 410, 634, 793, 1296, 2603, 2609
CseI GACGC 2 cut(s) 792, 897
CsiI ACCWGGT 2 cut(s) 2011, 2605
Csp6I GTAC 7 cut(s) 268, 487, 508, 644, 1385, 2832, 2892
CspCI CAANNNNNGTGG 1 cut(s) 32
CviQI GTAC 7 cut(s) 268, 487, 508, 644, 1385, 2832, 2892
DdeI CTNAG 9 cut(s) 390, 912, 1327, 1641, 1724, 1882, 2277, 2903, 3030
DraI TTTAAA 1 cut(s) 664
DraIII CACNNNGTG 1 cut(s) 772
EaeI YGGCCR 1 cut(s) 2156
Eam1104I CTCTTC 3 cut(s) 338, 1136, 1770
EarI CTCTTC 3 cut(s) 338, 1136, 1770
Ecl136II GAGCTC 1 cut(s) 309
Eco130I CCWWGG 1 cut(s) 1431
Eco147I AGGCCT 1 cut(s) 2090
Eco24I GRGCYC 2 cut(s) 311, 2852
Eco31I GGTCTC 1 cut(s) 2040
Eco32I GATATC 1 cut(s) 2029
Eco47I GGWCC 5 cut(s) 410, 793, 1296, 2603, 2609
Eco53kI GAGCTC 1 cut(s) 309
Eco57I CTGAAG 6 cut(s) 252, 721, 813, 2484, 2661, 2963
EcoICRI GAGCTC 1 cut(s) 309
EcoRI GAATTC 4 cut(s) 61, 1792, 2005, 2053
EcoRII CCWGG 5 cut(s) 1347, 1650, 2011, 2605, 2671
EcoRV GATATC 1 cut(s) 2029
EcoT14I CCWWGG 1 cut(s) 1431
EcoT22I ATGCAT 2 cut(s) 208, 1625
EcoT38I GRGCYC 2 cut(s) 311, 2852
ErhI CCWWGG 1 cut(s) 1431
Esp3I CGTCTC 1 cut(s) 2309
FalI AAGNNNNNCTT 2 cut(s) 1794, 1826
FaqI GGGAC 1 cut(s) 790
FblI GTMKAC 1 cut(s) 522
Fnu4HI GCNGC 5 cut(s) 351, 781, 2235, 2509, 2736
FriOI GRGCYC 2 cut(s) 311, 2852
Fsp4HI GCNGC 5 cut(s) 351, 781, 2235, 2509, 2736
FspBI CTAG 5 cut(s) 932, 1013, 1337, 1628, 2591
GluI GCNGC 5 cut(s) 351, 781, 2235, 2509, 2736
HaeIII GGCC 6 cut(s) 407, 635, 2090, 2158, 2497, 2521
HapII CCGG 3 cut(s) 408, 1134, 2165
HgaI GACGC 2 cut(s) 792, 897
HincII GTYRAC 3 cut(s) 675, 2932, 2965
HindII GTYRAC 3 cut(s) 675, 2932, 2965
HpaII CCGG 3 cut(s) 408, 1134, 2165
HphI GGTGA 6 cut(s) 31, 1061, 1744, 2001, 2670, 3034
Hpy188III TCNNGA 6 cut(s) 538, 932, 947, 1013, 1901, 2639
HpyAV CCTTC 8 cut(s) 647, 962, 1966, 1995, 2508, 2583, 2862, 2987
HpyCH4III ACNGT 7 cut(s) 246, 1194, 1669, 1864, 2133, 2274, 2836
HpyCH4IV ACGT 2 cut(s) 517, 2934
HpyF10VI GCNNNNNNNGC 8 cut(s) 501, 704, 786, 875, 1637, 1874, 1883, 2805
HpyF3I CTNAG 9 cut(s) 390, 912, 1327, 1641, 1724, 1882, 2277, 2903, 3030
HpySE526I ACGT 2 cut(s) 517, 2934
LmnI GCTCC 2 cut(s) 382, 2047
Lsp1109I GCAGC 3 cut(s) 337, 792, 2747
LweI GCATC 9 cut(s) 1021, 1223, 1610, 1924, 2256, 2650, 2770, 3011, 3026
MabI ACCWGGT 2 cut(s) 2011, 2605
MaeI CTAG 5 cut(s) 932, 1013, 1337, 1628, 2591
MaeII ACGT 2 cut(s) 517, 2934
MaeIII GTNAC 4 cut(s) 1369, 1447, 1669, 2096
MfeI CAATTG 1 cut(s) 1179
MflI RGATCY 3 cut(s) 943, 1118, 2860
MhlI GDGCHC 4 cut(s) 311, 1268, 1396, 2852
MlyI GAGTC 3 cut(s) 13, 1074, 3035
MmeI TCCRAC 6 cut(s) 117, 579, 1674, 2174, 2581, 2636
Mph1103I ATGCAT 2 cut(s) 208, 1625
MroXI GAANNNNTTC 2 cut(s) 170, 1764
MslI CAYNNNNRTG 3 cut(s) 1224, 1259, 1271
MspA1I CMGCKG 1 cut(s) 572
MspCI CTTAAG 1 cut(s) 2092
MspI CCGG 3 cut(s) 408, 1134, 2165
MspR9I CCNGG 6 cut(s) 1349, 1652, 2013, 2166, 2607, 2673
MunI CAATTG 1 cut(s) 1179
MvaI CCWGG 5 cut(s) 1349, 1652, 2013, 2607, 2673
MvnI CGCG 1 cut(s) 2896
MwoI GCNNNNNNNGC 8 cut(s) 501, 704, 786, 875, 1637, 1874, 1883, 2805
NciI CCSGG 1 cut(s) 2166
NheI GCTAGC 1 cut(s) 1627
NlaIV GGNNCC 7 cut(s) 945, 984, 1120, 1346, 1613, 1840, 1983
NmuCI GTSAC 1 cut(s) 1669
NsiI ATGCAT 2 cut(s) 208, 1625
NspI RCATGY 4 cut(s) 650, 1577, 1627, 2187
NspV TTCGAA 2 cut(s) 2476, 2855
OliI CACNNNNGTG 1 cut(s) 1259
PaeI GCATGC 2 cut(s) 1577, 1627
PaqCI CACCTGC 1 cut(s) 19
PceI AGGCCT 1 cut(s) 2090
PciI ACATGT 2 cut(s) 646, 2183
PcsI WCGNNNNNNNCGW 1 cut(s) 523
PdmI GAANNNNTTC 2 cut(s) 170, 1764
PfeI GAWTC 7 cut(s) 83, 758, 1154, 1760, 2244, 2951, 2983
PflFI GACNNNGTC 1 cut(s) 2607
PfoI TCCNGGA 2 cut(s) 2164, 2671
PkrI GCNGC 5 cut(s) 352, 782, 2236, 2510, 2737
PleI GAGTC 3 cut(s) 12, 1073, 3035
PpsI GAGTC 3 cut(s) 12, 1073, 3035
PscI ACATGT 2 cut(s) 646, 2183
Psp124BI GAGCTC 1 cut(s) 311
Psp6I CCWGG 5 cut(s) 1347, 1650, 2011, 2605, 2671
PspGI CCWGG 5 cut(s) 1347, 1650, 2011, 2605, 2671
PspN4I GGNNCC 7 cut(s) 945, 984, 1120, 1346, 1613, 1840, 1983
PspPI GGNCC 6 cut(s) 410, 634, 793, 1296, 2603, 2609
PstI CTGCAG 1 cut(s) 2737
PstNI CAGNNNCTG 2 cut(s) 911, 2195
PsuI RGATCY 3 cut(s) 943, 1118, 2860
PsyI GACNNNGTC 1 cut(s) 2607
PvuII CAGCTG 1 cut(s) 572
RsaI GTAC 7 cut(s) 269, 488, 509, 645, 1386, 2833, 2893
RsaNI GTAC 7 cut(s) 268, 487, 508, 644, 1385, 2832, 2892
RseI CAYNNNNRTG 3 cut(s) 1224, 1259, 1271
SacI GAGCTC 1 cut(s) 311
SatI GCNGC 5 cut(s) 351, 781, 2235, 2509, 2736
Sau96I GGNCC 6 cut(s) 410, 634, 793, 1296, 2603, 2609
ScaI AGTACT 2 cut(s) 488, 509
SchI GAGTC 3 cut(s) 13, 1074, 3035
ScrFI CCNGG 6 cut(s) 1349, 1652, 2013, 2166, 2607, 2673
SduI GDGCHC 4 cut(s) 311, 1268, 1396, 2852
SexAI ACCWGGT 2 cut(s) 2011, 2605
SfaNI GCATC 9 cut(s) 1021, 1223, 1610, 1924, 2256, 2650, 2770, 3011, 3026
SfcI CTRYAG 3 cut(s) 1158, 1549, 2733
SfuI TTCGAA 2 cut(s) 2476, 2855
SinI GGWCC 5 cut(s) 410, 793, 1296, 2603, 2609
SmiMI CAYNNNNRTG 3 cut(s) 1224, 1259, 1271
SmlI CTYRAG 4 cut(s) 455, 490, 1901, 2092
SmoI CTYRAG 4 cut(s) 455, 490, 1901, 2092
SphI GCATGC 2 cut(s) 1577, 1627
SseBI AGGCCT 1 cut(s) 2090
SsiI CCGC 6 cut(s) 878, 2234, 2350, 2465, 2509, 2881
SspI AATATT 1 cut(s) 2565
SspMI CTAG 5 cut(s) 932, 1013, 1337, 1628, 2591
SstI GAGCTC 1 cut(s) 311
StuI AGGCCT 1 cut(s) 2090
StyD4I CCNGG 6 cut(s) 1347, 1650, 2011, 2164, 2605, 2671
StyI CCWWGG 1 cut(s) 1431
TaaI ACNGT 7 cut(s) 246, 1194, 1669, 1864, 2133, 2274, 2836
TaiI ACGT 2 cut(s) 520, 2937
TaqI TCGA 6 cut(s) 103, 1844, 2358, 2476, 2595, 2855
TatI WGTACW 4 cut(s) 267, 486, 507, 643
TauI GCSGC 2 cut(s) 2237, 2511
TfiI GAWTC 7 cut(s) 83, 758, 1154, 1760, 2244, 2951, 2983
TscAI CASTG 6 cut(s) 1197, 1266, 1273, 1695, 2872, 2953
TseFI GTSAC 1 cut(s) 1669
TseI GCWGC 3 cut(s) 350, 780, 2735
Tsp45I GTSAC 1 cut(s) 1669
TspDTI ATGAA 9 cut(s) 179, 374, 710, 1060, 1139, 1735, 1785, 2557, 2574
TspGWI ACGGA 4 cut(s) 237, 262, 1305, 2344
TspRI CASTG 6 cut(s) 1197, 1266, 1273, 1695, 2872, 2953
Tth111I GACNNNGTC 1 cut(s) 2607
Vha464I CTTAAG 1 cut(s) 2092
VpaK11BI GGWCC 5 cut(s) 410, 793, 1296, 2603, 2609
XbaI TCTAGA 2 cut(s) 931, 1012
XceI RCATGY 4 cut(s) 650, 1577, 1627, 2187
XcmI CCANNNNNNNNNTGG 1 cut(s) 1927
XmiI GTMKAC 1 cut(s) 522
XmnI GAANNNNTTC 2 cut(s) 170, 1764
XspI CTAG 5 cut(s) 932, 1013, 1337, 1628, 2591
ZrmI AGTACT 2 cut(s) 488, 509
Zsp2I ATGCAT 2 cut(s) 208, 1625
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.