pycom07g03280

Retinoblastoma-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0003674
Physical Location & Seq
Forward (+)
2730450 .. 2738311
7862 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g03280.9

Sequence Viewer

Length: 3366 bp
ATGTATGGGAGTCACGATGGGATGCTATGTTGTTTTTCCCGCGCGCCCAAAAAGAGGCCTGAAAACCAAACAAATTTAGAGAGAGAGAAGGGAAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGACAGAGATAGGGTTCCACAATCACAGAAAGAGAGAAGAAGAAGAGAGAGAGAGAGAGCTTCGGCTTCAGAGAACGCCGTCGTTTTGGGTAACATTGGCAGATCTGCTGCTCTCCGAGAGCTCCGACTACGATATCAGATAGCTAGGGTTCAGATGAGTCCACCTGCTATGGTGAAGATGGAAGATACTAAGCCTGAAGTTTCAGCTTCCAACAATTCAAATTCCGAGAGCAGCGATTCTGGTCCCGTCCAAGATCGATTTACTGATTTGTGCAAGAATGTATTATCATTGGACGAAAACTCTTTTGCGGAAGCTGCAAAGCTGTTCAAAGAAACCAAACATCTGTTGATATCAAATGCGTCAGCTATTGGCAACGGAACACCTGAAGAAGCAGAACGATTCTGGTTTGCCTTTGTTTTGTACTCTGTAAAAACATTGAATGACAAGAATTCGGATAATTCACAGAAGAGTTCTGATGATAATGGATTTTCTCTCTTCCAAATTTTGAGAGCTGCAAAGTTGAACATCGTAGATTTTTTCAAGGAGCTTCCTCAGTTTGTTGTTAAAGCTGGTTCAATTTTAAGTAATATTTATGGTGTAGATTGGGAGAACAAACTTGAGGCAAAAGAGCTGCAGGCAAATTTTGTGTACTTGAGCTCGTTAAGCAAGTACTACAAACGTGCATATCGGGAGTTATTCTTCACAAGTGATACAACTGCCGACAAGCAGTCAACTGTTGCCAGTGGAACTGGTTATGTGTCTGAGTATCATCGTTTTGGATGGTTGCTGTTTTTGGCCCTTCGTGTACACGTATTCAGCCGATTTAAAGACCTGGTGACTTGCACAAACGGCTTGGTTGCAATATTGGGAATTTTAATAATTCATGTCCCTGTTCGCTTCAGAAAGTTCAGCATCCATGATTCAACACGCTTTGCGAGGAAAGGCAACAAAGGTGTGGACCTGCTTGCATGCCTCTGCAACACTTATGACACCTCAGAAGATGAGTTGAGAAAAACAATGGAAATGGCTAACGGTTTAATAGCAGAAATTTTGAAGAAGAAGCCCATTTCGGCGTCTGATTATAAAACGGAAAATCTAGAGAATATGGATCCAGATGGTTTGACGTACTTTGAAGGTCTTATGGAGGAACCATCTTTGTTGTCCAGTTTAGAGATTCTAGAAAAAGATTACGATGATGCAATTAGGAATAAGGGAGAAGTTGATGAGAGAGTCTTCATCAACGAGGACGATAGCTTAATTGGTTCATGGAGCTTTTCAGGACGATCCATGAGTGTTGCCGGTGTGAAGAGGAAATTTGATTCCATGGCCTCCCCAACAAAGACAATTATAAGTCCACTCTCTCCCCGTCGCTCTCCTGCATCTAATGTCAGTGGTGGTGCTAATTCAAAGATGGTGGCTACACCTGTGAGTACTGCAATGACAACTGCAAAGTGGCTTCGGACCTTTATTTCTCCGCTTCCTTCACAACCCTCAGCTGAGCTGGAGGGGTTCCTGGCATTGTGTGATAGGGATGTTACTACTGATGTGATACGTAGGGCACAAATTATATTGGAGGCCATATTTCCATGTAGTGCATTGGGAGAGCGATGTGTAAGTGGTAGCCTGCAAGGTGCTAACCTCATGGACAACATCTGGTCAGAGCAACGAAGATTGGAAGCACTCAAACTTTACTATAGAGTTTTGGAGGCAATGTGTAGGGCAGAGGCTCAAGTACTGCATGCAACTAATTTGACCTCCTTGTTAACCAATGAGAGGTTCCACAGATGCATGTTAGCCTGCTCTGGAGAGTTAGTCCTGGCAACACATAAGACTGTCACAATGTTGTTTCCAGCAGTATTAGTGAGGACGGGTATTACCGCTTTTGATCTTAGCAAGGTGATAGAGAGTTTCATTAGACATGAGGAATCTCTCCCAAGAGAATTAAGAAGACATCTGAATTCATTGGAAGAACGACTTTTGGAGAGCATGGTTTGGGAGAAGGGTTCCTCGATGTACAATTCTTTAACTGTTGCAAGACCTGCCCTCTCAGCAGAAATAAATCGTCTTGGGCTATTAGCAGAGCCCATGCCATCTTTGGATGCAATTGCCATGAATATTAACTTCCCTTGTGGAGGGTTGCCTCCAGTACCTTATTTACAGAAGCATGAGACTTCACCAGGCCAGAATGGAGATATCAGGTCTCCAAAGAGATCCTGCACAGATTATAGGAGTATATTGCTGGAGCGGAATTCCTTTACATCTCCCGTGAAGGATCGTCCTCTGGCGTTAAGTAACCTTAAGTCAAAGCTGCCACCGCCTCCTTTACAATCTGCATTTGCCAGTCCAACTCGACCAAACCCAGGAGGCGGAGGGGAAACATGTGCAGAAACTGGAATTAGTATTTTCTTTAGCAAGATAATCAAGCTGGCAGCTGTCAGAATCAATGGTATGGTTGAAAGAATGCAACTGTCTCAGCAGATAAGAGAGAATGTCTATCGTCTCTTTCAACAAATACTTGTTCAGCGGACATCTCTCTTTTTTAACCGCCATATTGACCAGATTATCCTTTGCTGTTTTTATGGAGTTTCAAAGATTTCTCAACTAAATCTAACATTTAGGGAAATCATATACAACTACAGAAAGCAACCACAATGTAAACCGCAAGTTTTTCGAAGCGTGTTTGTTGAGTGGCCTTCAGCACGCCGTAATGGGAGGCCCGCACAGGAACACGTGGATATCATAACATTTTACAATGAAATATTCATACCTTCTGTGAAGACCCTGCTAGTTGAGCTTGGACCTGCTGTTACAACAACTACAAGAGCTAACATGGTTCCTGAAGTTAACAATAATAATGATGCTCCATGCCCTGGATCACCTAAAGTATCTACTTTTCCAAGTCTTCCTGATATGTCACCAAAGAAAGTTTCTGCAGCGCACAATGTTTATGTCTCTCCATTGCGATCATCTAAGATGGATGCTTTGATCTCACATAGCTCAAAAAGCTATTATGCTTGTGTTGGGGAGAGTACTCATGCTTATCAGAGCCCTTCGAAAGATCTCACTGCCATCAACAACCGCTTGAACGGTACCCGAAAGCTCAGAGGCACTCTCAACTTCGACAGCGTTGATGTAGGCCTGGTCAGTGATTCGATGGTGGCCAACAGCCTGTACCTTCAAAATGGGAGTTGTGCATCCTCATCCGGTGCTCCAGTGAAAACTGAGCAACCTGACTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000082 GO:0000278 GO:0000280 GO:0000902 GO:0000904 GO:0001558 GO:0001708 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006275 GO:0006349 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007059 GO:0007127 GO:0007129 GO:0007275 GO:0008134 GO:0008150 GO:0008152 GO:0008356 GO:0009553 GO:0009555 GO:0009566 GO:0009567 GO:0009653 GO:0009791 GO:0009888 GO:0009889 GO:0009987 GO:0010016 GO:0010026 GO:0010052 GO:0010090 GO:0010103 GO:0010374 GO:0010377 GO:0010468 GO:0010556 GO:0010564 GO:0016043 GO:0019219 GO:0019222 GO:0019953 GO:0022402 GO:0022414 GO:0022619 GO:0030154 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032875 GO:0032989 GO:0033043 GO:0034641 GO:0040008 GO:0040029 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0044770 GO:0044772 GO:0044843 GO:0045132 GO:0045143 GO:0045165 GO:0046483 GO:0048229 GO:0048285 GO:0048366 GO:0048367 GO:0048468 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051052 GO:0051128 GO:0051171 GO:0051239 GO:0051276 GO:0051301 GO:0051321 GO:0051704 GO:0051726 GO:0051783 GO:0055046 GO:0060255 GO:0061982 GO:0065007 GO:0070192 GO:0071514 GO:0071704 GO:0071840 GO:0080090 GO:0090329 GO:0090558 GO:0090626 GO:0090627 GO:0090698 GO:0097159 GO:0098813 GO:0099402 GO:0140013 GO:1901360 GO:1901363 GO:1903046 GO:1903047 GO:1903866 GO:2000036 GO:2000112 GO:2000653
KEGG Pathways
Metabolic & Signaling
ko04110 ko04218 ko04350 ko05165 ko05203 map04110 map04218 map04350 map05165 map05203
Pfam Domains
Protein Families
DUF3452 RB_A RB_B Rb_C

Protein Analysis

1122

Amino Acids

125.44

Weight (kDa)

8.9

Isoelectric Point (pI)

53.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1209, 1475
AarI CACCTGC 1 cut(s) 298
Acc36I ACCTGC 4 cut(s) 298, 1095, 2173, 2935
Acc65I GGTACC 1 cut(s) 3215
AccB1I GGYRCC 1 cut(s) 3215
AccB7I CCANNNNNTGG 1 cut(s) 2996
AccBSI CCGCTC 1 cut(s) 2371
AccII CGCG 2 cut(s) 42, 44
AclWI GGATC 6 cut(s) 1229, 1242, 1404, 2331, 2406, 3007
AcoI YGGCCR 1 cut(s) 3285
AcuI CTGAAG 6 cut(s) 177, 342, 531, 1009, 2805, 2985
AcvI CACGTG 1 cut(s) 2857
AcyI GRCGYC 1 cut(s) 1199
AfiI CCNNNNNNNGG 6 cut(s) 54, 1899, 2258, 2486, 2492, 2996
AflII CTTAAG 1 cut(s) 2423
AflIII ACRYGT 3 cut(s) 934, 2504, 2854
AhdI GACNNNNNGTC 1 cut(s) 853
AjnI CCWGG 7 cut(s) 957, 1638, 1941, 2302, 2485, 2995, 3264
AjuI GAANNNNNNNTTGG 4 cut(s) 1886, 1918, 3037, 3069
Alw21I GWGCWC 3 cut(s) 249, 785, 3337
Alw26I GTCTC 6 cut(s) 120, 2288, 2331, 2601, 2630, 3082
AlwI GGATC 6 cut(s) 1229, 1242, 1404, 2331, 2406, 3007
AlwNI CAGNNNCTG 1 cut(s) 2516
AoxI GGCC 9 cut(s) 56, 921, 1452, 1701, 2305, 2816, 2840, 3262, 3285
ApeKI GCWGC 8 cut(s) 233, 357, 440, 638, 757, 2434, 2555, 3059
Asp718I GGTACC 1 cut(s) 3215
AspLEI GCGC 3 cut(s) 44, 46, 3064
AspS9I GGNCC 6 cut(s) 368, 922, 1084, 1587, 2841, 2924
AsuHPI GGTGA 6 cut(s) 310, 973, 2035, 2292, 2994, 3033
AsuII TTCGAA 2 cut(s) 2797, 3179
AvaII GGWCC 4 cut(s) 368, 1084, 1587, 2924
BaeGI GKGCMC 1 cut(s) 1687
BalI TGGCCA 1 cut(s) 3287
BamHI GGATCC 1 cut(s) 1234
BanI GGYRCC 1 cut(s) 3215
BanII GRGCYC 4 cut(s) 249, 785, 2211, 3176
BbrPI CACGTG 1 cut(s) 2857
BbsI GAAGAC 4 cut(s) 1351, 2080, 2909, 3020
Bbv12I GWGCWC 3 cut(s) 249, 785, 3337
BbvCI CCTCAGC 1 cut(s) 1618
BbvI GCAGC 8 cut(s) 220, 369, 427, 625, 744, 2421, 2567, 3071
BceAI ACGGC 3 cut(s) 188, 991, 2814
BcgI CGANNNNNNTGC 2 cut(s) 2777, 2811
BciT130I CCWGG 7 cut(s) 959, 1640, 1943, 2304, 2487, 2997, 3266
BcoDI GTCTC 6 cut(s) 120, 2288, 2331, 2601, 2630, 3082
BfaI CTAG 4 cut(s) 270, 1223, 1304, 2912
BfmI CTRYAG 4 cut(s) 758, 1819, 2761, 3057
BfrI CTTAAG 1 cut(s) 2423
BfuAI ACCTGC 4 cut(s) 298, 1095, 2173, 2935
BglII AGATCT 2 cut(s) 227, 3184
BisI GCNGC 8 cut(s) 234, 358, 441, 639, 758, 2435, 2556, 3060
BlpI GCTNAGC 1 cut(s) 1623
BlsI GCNGC 8 cut(s) 235, 359, 442, 640, 759, 2436, 2557, 3061
BmcAI AGTACT 4 cut(s) 797, 1558, 1860, 3157
Bme1390I CCNGG 7 cut(s) 959, 1640, 1943, 2304, 2487, 2997, 3266
Bme18I GGWCC 4 cut(s) 368, 1084, 1587, 2924
BmeRI GACNNNNNGTC 1 cut(s) 853
BmgT120I GGNCC 6 cut(s) 368, 922, 1084, 1587, 2841, 2924
BmiI GGNNCC 9 cut(s) 141, 370, 1236, 1275, 1637, 1904, 2131, 2961, 3217
BmrFI CCNGG 7 cut(s) 959, 1640, 1943, 2304, 2487, 2997, 3266
BmsI GCATC 9 cut(s) 12, 1047, 1312, 1514, 1901, 2215, 2974, 3094, 3329
BpiI GAAGAC 4 cut(s) 1351, 2080, 2909, 3020
BplI GAGNNNNNCTC 2 cut(s) 3222, 3254
BpmI CTGGAG 5 cut(s) 1649, 1950, 2253, 2387, 3321
Bpu10I CCTNAGC 1 cut(s) 1618
Bpu1102I GCTNAGC 1 cut(s) 1623
Bpu14I TTCGAA 2 cut(s) 2797, 3179
BpuEI CTTGAG 3 cut(s) 764, 799, 1839
Bsa29I ATCGAT 1 cut(s) 382
BsaAI YACGTR 3 cut(s) 937, 1679, 2857
BsaHI GRCGYC 1 cut(s) 1199
BsaI GGTCTC 1 cut(s) 2331
BsaJI CCNNGG 3 cut(s) 1449, 2485, 2995
BsaWI WCCGGW 1 cut(s) 3329
BsaXI ACNNNNNCTCC 4 cut(s) 1823, 1853, 1923, 1953
Bsc4I CCNNNNNNNGG 6 cut(s) 54, 1899, 2258, 2486, 2492, 2996
Bse118I RCCGGY 1 cut(s) 1424
Bse1I ACTGG 7 cut(s) 867, 880, 1290, 2270, 2466, 2521, 3338
Bse3DI GCAATG 3 cut(s) 1569, 1842, 3083
BseBI CCWGG 7 cut(s) 959, 1640, 1943, 2304, 2487, 2997, 3266
BseCI ATCGAT 1 cut(s) 382
BseDI CCNNGG 3 cut(s) 1449, 2485, 2995
BseGI GGATG 8 cut(s) 27, 911, 1038, 1663, 2230, 3109, 3320, 3326
BseLI CCNNNNNNNGG 6 cut(s) 54, 1899, 2258, 2486, 2492, 2996
BseMI GCAATG 3 cut(s) 1569, 1842, 3083
BseMII CTCAG 9 cut(s) 692, 879, 1134, 1614, 1632, 2187, 2612, 3241, 3339
BseNI ACTGG 7 cut(s) 867, 880, 1290, 2270, 2466, 2521, 3338
BsePI GCGCGC 1 cut(s) 42
BseSI GKGCMC 1 cut(s) 1687
BseXI GCAGC 8 cut(s) 220, 369, 427, 625, 744, 2421, 2567, 3071
BsgI GTGCAG 2 cut(s) 2326, 2529
Bsh1236I CGCG 2 cut(s) 42, 44
BshFI GGCC 9 cut(s) 58, 923, 1454, 1703, 2307, 2818, 2842, 3264, 3287
BshNI GGYRCC 1 cut(s) 3215
BshVI ATCGAT 1 cut(s) 382
BsiHKAI GWGCWC 3 cut(s) 249, 785, 3337
BsiSI CCGG 2 cut(s) 1425, 3330
BslFI GGGAC 2 cut(s) 354, 998
BslI CCNNNNNNNGG 6 cut(s) 54, 1899, 2258, 2486, 2492, 2996
BsmAI GTCTC 6 cut(s) 120, 2288, 2331, 2601, 2630, 3082
BsmBI CGTCTC 1 cut(s) 2630
BsmFI GGGAC 2 cut(s) 354, 998
BsmI GAATGC 1 cut(s) 2592
BsnI GGCC 9 cut(s) 58, 923, 1454, 1703, 2307, 2818, 2842, 3264, 3287
Bso31I GGTCTC 1 cut(s) 2331
Bsp119I TTCGAA 2 cut(s) 2797, 3179
Bsp1286I GDGCHC 6 cut(s) 249, 785, 1687, 2211, 3176, 3337
Bsp1407I TGTACA 2 cut(s) 931, 2139
Bsp1720I GCTNAGC 1 cut(s) 1623
Bsp19I CCATGG 1 cut(s) 1449
BspANI GGCC 9 cut(s) 58, 923, 1454, 1703, 2307, 2818, 2842, 3264, 3287
BspCNI CTCAG 9 cut(s) 691, 880, 1133, 1615, 1631, 2186, 2611, 3240, 3340
BspDI ATCGAT 1 cut(s) 382
BspFNI CGCG 2 cut(s) 42, 44
BspHI TCATGA 1 cut(s) 3362
BspLI GGNNCC 9 cut(s) 141, 370, 1236, 1275, 1637, 1904, 2131, 2961, 3217
BspMAI CTGCAG 2 cut(s) 762, 3061
BspMI ACCTGC 4 cut(s) 298, 1095, 2173, 2935
BspPI GGATC 6 cut(s) 1229, 1242, 1404, 2331, 2406, 3007
BspT104I TTCGAA 2 cut(s) 2797, 3179
BspT107I GGYRCC 1 cut(s) 3215
BspTI CTTAAG 1 cut(s) 2423
BspTNI GGTCTC 1 cut(s) 2331
BsrBI CCGCTC 1 cut(s) 2371
BsrDI GCAATG 3 cut(s) 1569, 1842, 3083
BsrFI RCCGGY 1 cut(s) 1424
BsrGI TGTACA 2 cut(s) 931, 2139
BsrI ACTGG 7 cut(s) 867, 880, 1290, 2270, 2466, 2521, 3338
BssAI RCCGGY 1 cut(s) 1424
BssECI CCNNGG 3 cut(s) 1449, 2485, 2995
BssHII GCGCGC 1 cut(s) 42
BssNI GRCGYC 1 cut(s) 1199
BssT1I CCWWGG 1 cut(s) 1449
Bst2UI CCWGG 7 cut(s) 959, 1640, 1943, 2304, 2487, 2997, 3266
Bst4CI ACNGT 6 cut(s) 862, 1160, 1960, 2155, 2595, 3215
Bst6I CTCTTC 5 cut(s) 88, 163, 587, 626, 1427
BstACI GRCGYC 1 cut(s) 1199
BstAFI CTTAAG 1 cut(s) 2423
BstAPI GCANNNNNTGC 1 cut(s) 2165
BstAUI TGTACA 2 cut(s) 931, 2139
BstBAI YACGTR 3 cut(s) 937, 1679, 2857
BstBI TTCGAA 2 cut(s) 2797, 3179
BstDSI CCRYGG 1 cut(s) 1449
BstENI CCTNNNNNAGG 1 cut(s) 2256
BstF5I GGATG 8 cut(s) 27, 911, 1038, 1663, 2230, 3109, 3320, 3326
BstFNI CGCG 2 cut(s) 42, 44
BstHHI GCGC 3 cut(s) 44, 46, 3064
BstMAI GTCTC 6 cut(s) 120, 2288, 2331, 2601, 2630, 3082
BstMWI GCNNNNNNNGC 8 cut(s) 440, 789, 975, 2165, 2174, 2440, 2917, 3129
BstNI CCWGG 7 cut(s) 959, 1640, 1943, 2304, 2487, 2997, 3266
BstNSI RCATGY 4 cut(s) 1098, 1868, 1918, 2508
BstSCI CCNGG 7 cut(s) 957, 1638, 1941, 2302, 2485, 2995, 3264
BstSFI CTRYAG 4 cut(s) 758, 1819, 2761, 3057
BstSLI GKGCMC 1 cut(s) 1687
BstSNI TACGTA 1 cut(s) 1679
BstUI CGCG 2 cut(s) 42, 44
BstV1I GCAGC 8 cut(s) 220, 369, 427, 625, 744, 2421, 2567, 3071
BstV2I GAAGAC 4 cut(s) 1351, 2080, 2909, 3020
BstX2I RGATCY 4 cut(s) 227, 1234, 2336, 3184
BstYI RGATCY 4 cut(s) 227, 1234, 2336, 3184
Bsu15I ATCGAT 1 cut(s) 382
BsuRI GGCC 9 cut(s) 58, 923, 1454, 1703, 2307, 2818, 2842, 3264, 3287
BsuTUI ATCGAT 1 cut(s) 382
BtgI CCRYGG 1 cut(s) 1449
BtgZI GCGATG 1 cut(s) 1747
BtsCI GGATG 8 cut(s) 27, 911, 1038, 1663, 2230, 3109, 3320, 3326
BtsI GCAGTG 1 cut(s) 3189
BtsIMutI CAGTG 5 cut(s) 874, 1522, 3189, 3277, 3345
BveI ACCTGC 4 cut(s) 298, 1095, 2173, 2935
CaiI CAGNNNCTG 1 cut(s) 2516
CciI TCATGA 1 cut(s) 3362
CfoI GCGC 3 cut(s) 44, 46, 3064
Cfr10I RCCGGY 1 cut(s) 1424
Cfr13I GGNCC 6 cut(s) 368, 922, 1084, 1587, 2841, 2924
ClaI ATCGAT 1 cut(s) 382
CseI GACGC 2 cut(s) 474, 1188
CsiI ACCWGGT 1 cut(s) 957
CspCI CAANNNNNGTGG 2 cut(s) 1521, 1556
DraI TTTAAA 1 cut(s) 952
DriI GACNNNNNGTC 1 cut(s) 853
EaeI YGGCCR 1 cut(s) 3285
Eam1104I CTCTTC 5 cut(s) 88, 163, 587, 626, 1427
Eam1105I GACNNNNNGTC 1 cut(s) 853
EarI CTCTTC 5 cut(s) 88, 163, 587, 626, 1427
EciI GGCGGA 1 cut(s) 2508
Ecl136II GAGCTC 2 cut(s) 247, 783
Eco105I TACGTA 1 cut(s) 1679
Eco130I CCWWGG 1 cut(s) 1449
Eco147I AGGCCT 2 cut(s) 58, 3264
Eco24I GRGCYC 4 cut(s) 249, 785, 2211, 3176
Eco31I GGTCTC 1 cut(s) 2331
Eco32I GATATC 4 cut(s) 260, 477, 2320, 2863
Eco47I GGWCC 4 cut(s) 368, 1084, 1587, 2924
Eco53kI GAGCTC 2 cut(s) 247, 783
Eco57I CTGAAG 6 cut(s) 177, 342, 531, 1009, 2805, 2985
Eco72I CACGTG 1 cut(s) 2857
EcoICRI GAGCTC 2 cut(s) 247, 783
EcoNI CCTNNNNNAGG 1 cut(s) 2256
EcoRI GAATTC 3 cut(s) 574, 2083, 2374
EcoRII CCWGG 7 cut(s) 957, 1638, 1941, 2302, 2485, 2995, 3264
EcoRV GATATC 4 cut(s) 260, 477, 2320, 2863
EcoT14I CCWWGG 1 cut(s) 1449
EcoT22I ATGCAT 1 cut(s) 1916
EcoT38I GRGCYC 4 cut(s) 249, 785, 2211, 3176
ErhI CCWWGG 1 cut(s) 1449
Esp3I CGTCTC 1 cut(s) 2630
FalI AAGNNNNNCTT 2 cut(s) 2085, 2117
FaqI GGGAC 2 cut(s) 354, 998
FauI CCCGC 2 cut(s) 47, 2851
Fnu4HI GCNGC 8 cut(s) 234, 358, 441, 639, 758, 2435, 2556, 3060
FokI GGATG 8 cut(s) 34, 918, 1025, 1670, 2237, 3116, 3307, 3313
FriOI GRGCYC 4 cut(s) 249, 785, 2211, 3176
Fsp4HI GCNGC 8 cut(s) 234, 358, 441, 639, 758, 2435, 2556, 3060
FspBI CTAG 4 cut(s) 270, 1223, 1304, 2912
GlaI GCGC 3 cut(s) 43, 45, 3063
GluI GCNGC 8 cut(s) 234, 358, 441, 639, 758, 2435, 2556, 3060
GsuI CTGGAG 5 cut(s) 1649, 1950, 2253, 2387, 3321
HaeIII GGCC 9 cut(s) 58, 923, 1454, 1703, 2307, 2818, 2842, 3264, 3287
HapII CCGG 2 cut(s) 1425, 3330
HgaI GACGC 2 cut(s) 474, 1188
HhaI GCGC 3 cut(s) 44, 46, 3064
Hin1I GRCGYC 1 cut(s) 1199
Hin6I GCGC 3 cut(s) 42, 44, 3062
HinP1I GCGC 3 cut(s) 42, 44, 3062
HincII GTYRAC 3 cut(s) 858, 1890, 2971
HindII GTYRAC 3 cut(s) 858, 1890, 2971
HpaI GTTAAC 2 cut(s) 1890, 2971
HpaII CCGG 2 cut(s) 1425, 3330
HphI GGTGA 6 cut(s) 310, 973, 2035, 2292, 2994, 3033
Hpy99I CGWCG 2 cut(s) 209, 1497
HpyCH4III ACNGT 6 cut(s) 862, 1160, 1960, 2155, 2595, 3215
HpyCH4IV ACGT 5 cut(s) 805, 936, 1250, 1678, 2856
HpyF10VI GCNNNNNNNGC 8 cut(s) 440, 789, 975, 2165, 2174, 2440, 2917, 3129
HpySE526I ACGT 5 cut(s) 805, 936, 1250, 1678, 2856
Hsp92I GRCGYC 1 cut(s) 1199
HspAI GCGC 3 cut(s) 42, 44, 3062
KpnI GGTACC 1 cut(s) 3219
KspAI GTTAAC 2 cut(s) 1890, 2971
LmnI GCTCC 6 cut(s) 252, 670, 1395, 2368, 2992, 3340
Lsp1109I GCAGC 8 cut(s) 220, 369, 427, 625, 744, 2421, 2567, 3071
LweI GCATC 9 cut(s) 12, 1047, 1312, 1514, 1901, 2215, 2974, 3094, 3329
MabI ACCWGGT 1 cut(s) 957
MaeI CTAG 4 cut(s) 270, 1223, 1304, 2912
MaeII ACGT 5 cut(s) 805, 936, 1250, 1678, 2856
MaeIII GTNAC 8 cut(s) 11, 215, 961, 1660, 1960, 2417, 2932, 3039
MbiI CCGCTC 1 cut(s) 2371
MfeI CAATTG 1 cut(s) 2229
MflI RGATCY 4 cut(s) 227, 1234, 2336, 3184
MhlI GDGCHC 6 cut(s) 249, 785, 1687, 2211, 3176, 3337
MlsI TGGCCA 1 cut(s) 3287
MluNI TGGCCA 1 cut(s) 3287
MlyI GAGTC 4 cut(s) 19, 292, 1365, 3353
MmeI TCCRAC 3 cut(s) 274, 360, 2495
Mox20I TGGCCA 1 cut(s) 3287
Mph1103I ATGCAT 1 cut(s) 1916
MscI TGGCCA 1 cut(s) 3287
Msp20I TGGCCA 1 cut(s) 3287
MspA1I CMGCKG 3 cut(s) 1622, 2558, 2650
MspCI CTTAAG 1 cut(s) 2423
MspI CCGG 2 cut(s) 1425, 3330
MspR9I CCNGG 7 cut(s) 959, 1640, 1943, 2304, 2487, 2997, 3266
MunI CAATTG 1 cut(s) 2229
Mva1269I GAATGC 1 cut(s) 2592
MvaI CCWGG 7 cut(s) 959, 1640, 1943, 2304, 2487, 2997, 3266
MvnI CGCG 2 cut(s) 42, 44
MwoI GCNNNNNNNGC 8 cut(s) 440, 789, 975, 2165, 2174, 2440, 2917, 3129
NcoI CCATGG 1 cut(s) 1449
NlaIV GGNNCC 9 cut(s) 141, 370, 1236, 1275, 1637, 1904, 2131, 2961, 3217
NmuCI GTSAC 4 cut(s) 11, 961, 1960, 3039
NsiI ATGCAT 1 cut(s) 1916
NspI RCATGY 4 cut(s) 1098, 1868, 1918, 2508
NspV TTCGAA 2 cut(s) 2797, 3179
PaeI GCATGC 2 cut(s) 1098, 1868
PagI TCATGA 1 cut(s) 3362
PaqCI CACCTGC 1 cut(s) 298
PauI GCGCGC 1 cut(s) 42
PceI AGGCCT 2 cut(s) 58, 3264
PciI ACATGT 1 cut(s) 2504
PctI GAATGC 1 cut(s) 2592
PfeI GAWTC 8 cut(s) 362, 525, 1046, 1300, 1445, 2051, 2565, 3275
PflMI CCANNNNNTGG 1 cut(s) 2996
PkrI GCNGC 8 cut(s) 235, 359, 442, 640, 759, 2436, 2557, 3061
PleI GAGTC 4 cut(s) 18, 291, 1364, 3353
PmaCI CACGTG 1 cut(s) 2857
PmlI CACGTG 1 cut(s) 2857
PpsI GAGTC 4 cut(s) 18, 291, 1364, 3353
Ppu21I YACGTR 3 cut(s) 937, 1679, 2857
PscI ACATGT 1 cut(s) 2504
PsiI TTATAA 2 cut(s) 1209, 1475
Psp124BI GAGCTC 2 cut(s) 249, 785
Psp6I CCWGG 7 cut(s) 957, 1638, 1941, 2302, 2485, 2995, 3264
PspCI CACGTG 1 cut(s) 2857
PspGI CCWGG 7 cut(s) 957, 1638, 1941, 2302, 2485, 2995, 3264
PspN4I GGNNCC 9 cut(s) 141, 370, 1236, 1275, 1637, 1904, 2131, 2961, 3217
PspPI GGNCC 6 cut(s) 368, 922, 1084, 1587, 2841, 2924
PstI CTGCAG 2 cut(s) 762, 3061
PstNI CAGNNNCTG 1 cut(s) 2516
PsuI RGATCY 4 cut(s) 227, 1234, 2336, 3184
PteI GCGCGC 1 cut(s) 42
PvuII CAGCTG 2 cut(s) 1622, 2558
SacI GAGCTC 2 cut(s) 249, 785
SatI GCNGC 8 cut(s) 234, 358, 441, 639, 758, 2435, 2556, 3060
Sau96I GGNCC 6 cut(s) 368, 922, 1084, 1587, 2841, 2924
ScaI AGTACT 4 cut(s) 797, 1558, 1860, 3157
SchI GAGTC 4 cut(s) 19, 292, 1365, 3353
ScrFI CCNGG 7 cut(s) 959, 1640, 1943, 2304, 2487, 2997, 3266
SduI GDGCHC 6 cut(s) 249, 785, 1687, 2211, 3176, 3337
SexAI ACCWGGT 1 cut(s) 957
SfaNI GCATC 9 cut(s) 12, 1047, 1312, 1514, 1901, 2215, 2974, 3094, 3329
SfcI CTRYAG 4 cut(s) 758, 1819, 2761, 3057
SfuI TTCGAA 2 cut(s) 2797, 3179
SinI GGWCC 4 cut(s) 368, 1084, 1587, 2924
SmlI CTYRAG 4 cut(s) 743, 778, 1854, 2423
SmoI CTYRAG 4 cut(s) 743, 778, 1854, 2423
SnaBI TACGTA 1 cut(s) 1679
SphI GCATGC 2 cut(s) 1098, 1868
SseBI AGGCCT 2 cut(s) 58, 3264
SspI AATATT 4 cut(s) 715, 990, 2242, 2886
SspMI CTAG 4 cut(s) 270, 1223, 1304, 2912
SstI GAGCTC 2 cut(s) 249, 785
StuI AGGCCT 2 cut(s) 58, 3264
StyD4I CCNGG 7 cut(s) 957, 1638, 1941, 2302, 2485, 2995, 3264
StyI CCWWGG 1 cut(s) 1449
TaaI ACNGT 6 cut(s) 862, 1160, 1960, 2155, 2595, 3215
TaiI ACGT 5 cut(s) 808, 939, 1253, 1681, 2859
TaqI TCGA 7 cut(s) 382, 2135, 2476, 2797, 3179, 3246, 3278
TatI WGTACW 8 cut(s) 546, 774, 795, 931, 1556, 1858, 2139, 3155
TfiI GAWTC 8 cut(s) 362, 525, 1046, 1300, 1445, 2051, 2565, 3275
TscAI CASTG 5 cut(s) 874, 1522, 3196, 3277, 3345
TseFI GTSAC 4 cut(s) 11, 961, 1960, 3039
TseI GCWGC 8 cut(s) 233, 357, 440, 638, 757, 2434, 2555, 3059
Tsp45I GTSAC 4 cut(s) 11, 961, 1960, 3039
TspDTI ATGAA 8 cut(s) 998, 1351, 1380, 2026, 2076, 2252, 2878, 2895
TspGWI ACGGA 2 cut(s) 516, 1229
TspRI CASTG 5 cut(s) 874, 1522, 3196, 3277, 3345
Van91I CCANNNNNTGG 1 cut(s) 2996
Vha464I CTTAAG 1 cut(s) 2423
VpaK11BI GGWCC 4 cut(s) 368, 1084, 1587, 2924
XagI CCTNNNNNAGG 1 cut(s) 2256
XbaI TCTAGA 2 cut(s) 1222, 1303
XceI RCATGY 4 cut(s) 1098, 1868, 1918, 2508
XcmI CCANNNNNNNNNTGG 1 cut(s) 2218
XspI CTAG 4 cut(s) 270, 1223, 1304, 2912
ZrmI AGTACT 4 cut(s) 797, 1558, 1860, 3157
Zsp2I ATGCAT 1 cut(s) 1916
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.