pycom02g23780

Retinoblastoma-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0003674
Physical Location & Seq
Forward (+)
21904880 .. 21911966
7087 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g23780.14

Sequence Viewer

Length: 3069 bp
ATGGTGAAGATGGAAGATACTAAGCCTGAAGTTTCAGCTTCCAACAATTCAAATTCCGAGAGCAGCGATTCAGGGCCCGTCCAAGATCGATTTACTGATTTGTGCAAGAATGTATTGTCGTTGGACAAGAACTCTTACACACAAGCTGCGAAACTGTTCAAAGAAACCAAACATCTGTTAATATCAAATGCGTCAGCTATTGGCAACGGAACACCTGAAGAAGCAGAACGGATCTGGTTTGCCTTTGTTTTGTACTCTGTGAAAACATTGAGCAACAAGAATTCGGATAATTCTCAGAAGAGCTCCGATGATAATGGATTTTCTCTCTTCCAAATTTTGAGAGCTACGAAGCTGAACATTGTAGATTTTTTCAAGGAGCTTCCTCAGTTTGTTATTAAGGCTGGTTCAATTTTGAGTAATATTTATGGCGTAGATTGGGAAAACAAACTTGAGGCAAAAGAGCTGCAGGCAAATTTTGTGTACTTGAGCCTGTTAAGCAAGTACTACAAACGTGCGTATCGGGAGTTATTCTTGACAAGTGATACAAATGCCAATAAGCAGTCAGCTGTTGCCGGTGGAACTGGTTATGTGTCAGAGTATCATCGTTTTGGATGGTTGCTGTTTTTGGCCCTTCGTGTACACGTATTCAGCCGGTTTAAAGACCTGGTGACTTGCACAAACGGCTTGGTTGCAATTTTGGGAATTTTAATAATTCACGTCCCTGTTCGCTTCAGAAAGTTCAGCATCCATGATTCAACACGCTTTGCAAGGAAAGGCAACAAAGGTGTGGACCTGCTTGCAAGTCTTTGCAACACTTATTACACCTCAGACGATGAGTTGAGAAAAACAATGGAAATGGCTAATGGTTTAATAGCAGATATTTTGAAGAAGAAGCCCGTTTCGGCGTCTGATTGTAAAACTGAAAATCTAGAGAATATGGATCCAGATGGTTTGACGTACTTTGAAGGTCTCATGGAGGAACCATCTTTGTCGTCCAGTTTAGATATTCTAGAAAAAAATTACGATGATGCAATTCGGAATAAGGGTGAAGTTGATGAGAGAGTCTTCATCAACGAGGACGATAGCTTACTTGGTTCAGGGAGCTTTTCAGGACGATCCATGAGTGTAGCTGGTGTGAAGAGAAAATTTGATTCCATAGCTTCCCCAACAAAGACAATTATAAGTCCACTCTCTCCCCATCGCTCTCCTGCATCTCATGTGAATGGTGGTGCTAATTCAAAGATGGTGGCTACACCTGTGAGCACTGCAATGACAACTGCAAAGTGGCTTCGGACCTTTATTTCTCCGCTTCCGTCAAAACCCTCAGCTGAGCTCGAGGGGTTCCTGGCATCGTGTGATAGGGATGTTACTACTGATGTGATACGCAGGGCACAAATTATATTGGAGGCCATATTTCTAGATAGTGCCCTGGGAGAGCGATGCATAACTGGTAGCCTGCAAGGTGCAAACCTCATGGACAACATCTGGCAGGAGCAACGAAGATTGGAAGCACTCAAACTTTATTATAGAGTTTTGGAAGCAATGTGTAGGGCAGAGGCTCAAGTACTGCATGCAACTAATTTGACCTCCTTATTAACCAATGAGAGGTTTCACAGATGCATGTTAGCCTGCTCTGCAGAGTTAGTCCTGGCAACACATAAGACTGTCACAATGTTGTTTCCAGCAGTATTAGAGAGGACCGGTATTACCGCTTTTGATCTTAGCAAGGTGATAGAGAGTTTCATTAGACATGAGGAATCTCTCCCAAGAGAATTAAGAAGACATCTGAATTCATTGGAAGAACGACTTTTGGAGAGCATGGTATGGGAGAAGGGTTCCTCGATGTATAATTCTTTAACTGTTGCAAGACCTGCTCTCTCAGCAGAAATAAATCGTCTCGGGTTATTAGCAGAGCCCATGCCATCTTTGGATGCAATTGCCATGAATATTAACTTCCCTTGTGGAGGGTTGCCTCCAGTACCTTCTTTACAGAAGCATGAGACTTCACTAGGTCAGAATGGGGATATCAGGTCTCCAAAGAGATCCTGCACAGACTATAGGAGTATATTGCTGGAGCGGAATTCCTTTACATCTCCTGTGAAGGATCGTCTTCTGGCCTTAAGTAACCTTAAGTCAAAGCTGCCACCACCTCCTTTACAATCTGCATTTGCCAGTCCAACACGACCAAATCCAGGAGGTGGAGGGGAAACATGTGCAGAAACTGGAATCAGTATTTTCTTTAGCAAGATAAACAAGCTGGCGGCAGTCAGAATCAATGGTATGGTTGAAAGAATGCAACTGTCTCAGATAAGAGAGAATGTTTATCGTCTCTTTCAACAAATACTAGTTCAGCGGACATCTCTCTTTTTCAACCGCCATATTGATCAGATTATCCTTTGTTGTTTTTATGGAGTTTCAAAGATTTCTCAACTAAATCTGACATTTAGGGAAATCATATACAACTACAGAAAGCAACCACAATGTAAACCGGAAGTTTTTCGAGGCGTGTTTGTTGAGTGGCCTTCACCACGCCGTAATGGGAGACCCGCACAGGAACACGTGGATATCATAACATTTTACAATGAAATATTCATACCTTCTGTGAAGACCCTGCTAGTTGAGCTCGGACCTGCTGTTACAGCAACTACAAGAGCTAACGTGGTTCCTGAAGTTAACAACAATAATGATGCTCCATGCCCTGGATCACCTAAAGTATCTACTTTTCCTAGTCTTCCTGATATGTCTCCAAAGAAAGTTTCTGCAGTGCACAATGTTTACGTCTCTCCATTGCGATCATCTAAGATGGATGCTTTGATATCACATAGCTCAAAAAGCTATTATGCTTGTGTTGGGCAGAGTACTCATGCTAATCAGAGCCCTTCGAAAGATCTCACTGCCATCAACAACCGCTTGAACGGTACCCGAAAGCTCAGAGGCACTCTCAACTTCGACAACGTTGATGTAGGCTTGGTCAGTGATTCTATGGTGGCCAGCAGCCTGTACCTTCAAAATGGGAGTTGTGCATCCTCACCCGGTGCCCCAGTGAAAACTGAGCAACCTGACTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000082 GO:0000278 GO:0000280 GO:0000902 GO:0000904 GO:0001558 GO:0001708 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006275 GO:0006349 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007059 GO:0007127 GO:0007129 GO:0007275 GO:0008134 GO:0008150 GO:0008152 GO:0008356 GO:0009553 GO:0009555 GO:0009566 GO:0009567 GO:0009653 GO:0009791 GO:0009888 GO:0009889 GO:0009987 GO:0010016 GO:0010026 GO:0010052 GO:0010090 GO:0010103 GO:0010374 GO:0010377 GO:0010468 GO:0010556 GO:0010564 GO:0016043 GO:0019219 GO:0019222 GO:0019953 GO:0022402 GO:0022414 GO:0022619 GO:0030154 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032875 GO:0032989 GO:0033043 GO:0034641 GO:0040008 GO:0040029 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0044770 GO:0044772 GO:0044843 GO:0045132 GO:0045143 GO:0045165 GO:0046483 GO:0048229 GO:0048285 GO:0048366 GO:0048367 GO:0048468 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051052 GO:0051128 GO:0051171 GO:0051239 GO:0051276 GO:0051301 GO:0051321 GO:0051704 GO:0051726 GO:0051783 GO:0055046 GO:0060255 GO:0061982 GO:0065007 GO:0070192 GO:0071514 GO:0071704 GO:0071840 GO:0080090 GO:0090329 GO:0090558 GO:0090626 GO:0090627 GO:0090698 GO:0097159 GO:0098813 GO:0099402 GO:0140013 GO:1901360 GO:1901363 GO:1903046 GO:1903047 GO:1903866 GO:2000036 GO:2000112 GO:2000653
KEGG Pathways
Metabolic & Signaling
ko04110 ko04218 ko04350 ko05165 ko05203 map04110 map04218 map04350 map05165 map05203
Pfam Domains
Protein Families
DUF3452 RB_A RB_B Rb_C

Protein Analysis

1023

Amino Acids

113.42

Weight (kDa)

8.75

Isoelectric Point (pI)

50.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1181
Acc36I ACCTGC 3 cut(s) 801, 1879, 2638
Acc65I GGTACC 1 cut(s) 2918
AccB1I GGYRCC 2 cut(s) 2918, 3035
AccB7I CCANNNNNTGG 2 cut(s) 2198, 2699
AccBSI CCGCTC 1 cut(s) 2077
AciI CCGC 8 cut(s) 1307, 1710, 2077, 2261, 2353, 2374, 2547, 2908
AclI AACGTT 1 cut(s) 2955
AclWI GGATC 7 cut(s) 239, 935, 948, 1110, 2037, 2112, 2710
AcoI YGGCCR 1 cut(s) 2988
AcsI RAATTY 8 cut(s) 52, 280, 333, 472, 702, 1145, 1789, 2080
AcuI CTGAAG 4 cut(s) 48, 237, 715, 2688
AcvI CACGTG 1 cut(s) 2560
AcyI GRCGYC 1 cut(s) 905
AdeI CACNNNGTG 1 cut(s) 3035
AfiI CCNNNNNNNGG 5 cut(s) 1605, 1964, 2192, 2198, 2699
AflII CTTAAG 2 cut(s) 2119, 2129
AflIII ACRYGT 3 cut(s) 640, 2210, 2557
AgeI ACCGGT 1 cut(s) 1700
AhlI ACTAGT 1 cut(s) 2344
AjiI CACGTC 1 cut(s) 718
AjnI CCWGG 6 cut(s) 663, 1344, 1428, 1647, 2191, 2698
AjuI GAANNNNNNNTTGG 2 cut(s) 2740, 2772
Alw21I GWGCWC 5 cut(s) 305, 1265, 1335, 2625, 2769
Alw26I GTCTC 9 cut(s) 974, 1901, 1994, 2037, 2307, 2333, 2536, 2748, 2785
Alw44I GTGCAC 1 cut(s) 2765
AlwI GGATC 7 cut(s) 239, 935, 948, 1110, 2037, 2112, 2710
AlwNI CAGNNNCTG 1 cut(s) 2222
Ama87I CYCGRG 2 cut(s) 1334, 1898
AoxI GGCC 6 cut(s) 74, 627, 1407, 2115, 2519, 2988
ApaI GGGCCC 1 cut(s) 78
ApaLI GTGCAC 1 cut(s) 2765
ApeKI GCWGC 5 cut(s) 63, 146, 463, 2140, 2994
ApoI RAATTY 8 cut(s) 52, 280, 333, 472, 702, 1145, 1789, 2080
AsiGI ACCGGT 1 cut(s) 1700
Asp700I GAANNNNTTC 2 cut(s) 155, 2496
Asp718I GGTACC 1 cut(s) 2918
AspS9I GGNCC 7 cut(s) 74, 75, 628, 790, 1293, 1698, 2627
AsuC2I CCSGG 1 cut(s) 3033
AsuHPI GGTGA 7 cut(s) 16, 679, 1058, 1741, 2517, 2697, 3021
AsuII TTCGAA 1 cut(s) 2882
AvaI CYCGRG 2 cut(s) 1334, 1898
AvaII GGWCC 4 cut(s) 790, 1293, 1698, 2627
BaeGI GKGCMC 5 cut(s) 78, 1393, 1429, 2769, 3040
BaeI ACNNNNGTAYC 2 cut(s) 500, 533
BalI TGGCCA 1 cut(s) 2990
BamHI GGATCC 1 cut(s) 940
BanI GGYRCC 2 cut(s) 2918, 3035
BanII GRGCYC 6 cut(s) 78, 305, 1335, 1917, 2625, 2879
BbrPI CACGTG 1 cut(s) 2560
BbsI GAAGAC 5 cut(s) 1057, 1786, 2102, 2612, 2723
Bbv12I GWGCWC 5 cut(s) 305, 1265, 1335, 2625, 2769
BbvCI CCTCAGC 1 cut(s) 1324
BbvI GCAGC 5 cut(s) 75, 133, 450, 2127, 3006
BccI CCATC 9 cut(s) 4, 606, 941, 991, 1206, 1237, 1930, 2797, 2906
BceAI ACGGC 2 cut(s) 697, 2517
BciT130I CCWGG 6 cut(s) 665, 1346, 1430, 1649, 2193, 2700
BclI TGATCA 1 cut(s) 2383
BcnI CCSGG 1 cut(s) 3033
BcoDI GTCTC 9 cut(s) 974, 1901, 1994, 2037, 2307, 2333, 2536, 2748, 2785
BcuI ACTAGT 1 cut(s) 2344
BfaI CTAG 7 cut(s) 929, 1010, 1418, 2009, 2345, 2615, 2727
BfmI CTRYAG 5 cut(s) 464, 1635, 2056, 2464, 2760
BfrI CTTAAG 2 cut(s) 2119, 2129
BfuAI ACCTGC 3 cut(s) 801, 1879, 2638
BglII AGATCT 1 cut(s) 2887
BisI GCNGC 6 cut(s) 64, 147, 464, 2141, 2262, 2995
BlpI GCTNAGC 1 cut(s) 1329
BlsI GCNGC 6 cut(s) 65, 148, 465, 2142, 2263, 2996
BmcAI AGTACT 3 cut(s) 503, 1566, 2860
Bme1390I CCNGG 7 cut(s) 665, 1346, 1430, 1649, 2193, 2700, 3033
Bme18I GGWCC 4 cut(s) 790, 1293, 1698, 2627
BmeT110I CYCGRG 2 cut(s) 1334, 1898
BmgBI CACGTC 1 cut(s) 718
BmgT120I GGNCC 7 cut(s) 74, 75, 628, 790, 1293, 1698, 2627
BmiI GGNNCC 8 cut(s) 76, 942, 981, 1343, 1837, 2664, 2920, 3037
BmrFI CCNGG 7 cut(s) 665, 1346, 1430, 1649, 2193, 2700, 3033
BmrI ACTGGG 1 cut(s) 3035
BmuI ACTGGG 1 cut(s) 3035
BpiI GAAGAC 5 cut(s) 1057, 1786, 2102, 2612, 2723
BplI GAGNNNNNCTC 2 cut(s) 2925, 2957
BpmI CTGGAG 2 cut(s) 1959, 2093
Bpu10I CCTNAGC 1 cut(s) 1324
Bpu1102I GCTNAGC 1 cut(s) 1329
Bpu14I TTCGAA 1 cut(s) 2882
BpuEI CTTGAG 3 cut(s) 470, 505, 1545
BpuMI CCSGG 1 cut(s) 3033
Bsa29I ATCGAT 1 cut(s) 88
BsaAI YACGTR 2 cut(s) 643, 2560
BsaHI GRCGYC 1 cut(s) 905
BsaI GGTCTC 3 cut(s) 974, 2037, 2536
BsaJI CCNNGG 3 cut(s) 1428, 1429, 2698
BsaWI WCCGGW 2 cut(s) 1700, 2488
Bsc4I CCNNNNNNNGG 5 cut(s) 1605, 1964, 2192, 2198, 2699
Bse118I RCCGGY 3 cut(s) 572, 651, 1700
Bse1I ACTGG 7 cut(s) 586, 996, 1453, 1976, 2172, 2227, 3041
Bse3DI GCAATG 3 cut(s) 1275, 1548, 2786
BseBI CCWGG 6 cut(s) 665, 1346, 1430, 1649, 2193, 2700
BseCI ATCGAT 1 cut(s) 88
BseDI CCNNGG 3 cut(s) 1428, 1429, 2698
BseGI GGATG 6 cut(s) 617, 744, 1369, 1936, 2812, 3023
BseLI CCNNNNNNNGG 5 cut(s) 1605, 1964, 2192, 2198, 2699
BseMI GCAATG 3 cut(s) 1275, 1548, 2786
BseMII CTCAG 9 cut(s) 308, 398, 840, 1320, 1338, 1893, 2318, 2944, 3042
BseNI ACTGG 7 cut(s) 586, 996, 1453, 1976, 2172, 2227, 3041
BseSI GKGCMC 5 cut(s) 78, 1393, 1429, 2769, 3040
BseXI GCAGC 5 cut(s) 75, 133, 450, 2127, 3006
BsgI GTGCAG 2 cut(s) 2032, 2235
BshFI GGCC 6 cut(s) 76, 629, 1409, 2117, 2521, 2990
BshNI GGYRCC 2 cut(s) 2918, 3035
BshTI ACCGGT 1 cut(s) 1700
BshVI ATCGAT 1 cut(s) 88
BsiHKAI GWGCWC 5 cut(s) 305, 1265, 1335, 2625, 2769
BsiHKCI CYCGRG 2 cut(s) 1334, 1898
BsiSI CCGG 5 cut(s) 573, 652, 1701, 2489, 3033
BslFI GGGAC 1 cut(s) 704
BslI CCNNNNNNNGG 5 cut(s) 1605, 1964, 2192, 2198, 2699
BsmAI GTCTC 9 cut(s) 974, 1901, 1994, 2037, 2307, 2333, 2536, 2748, 2785
BsmBI CGTCTC 3 cut(s) 1901, 2333, 2785
BsmFI GGGAC 1 cut(s) 704
BsmI GAATGC 1 cut(s) 2298
BsnI GGCC 6 cut(s) 76, 629, 1409, 2117, 2521, 2990
Bso31I GGTCTC 3 cut(s) 974, 2037, 2536
BsoBI CYCGRG 2 cut(s) 1334, 1898
Bsp119I TTCGAA 1 cut(s) 2882
Bsp120I GGGCCC 1 cut(s) 74
Bsp1407I TGTACA 1 cut(s) 637
Bsp1720I GCTNAGC 1 cut(s) 1329
BspACI CCGC 8 cut(s) 1307, 1710, 2077, 2261, 2353, 2374, 2547, 2908
BspANI GGCC 6 cut(s) 76, 629, 1409, 2117, 2521, 2990
BspCNI CTCAG 9 cut(s) 307, 397, 839, 1321, 1337, 1892, 2317, 2943, 3043
BspDI ATCGAT 1 cut(s) 88
BspLI GGNNCC 8 cut(s) 76, 942, 981, 1343, 1837, 2664, 2920, 3037
BspMAI CTGCAG 3 cut(s) 468, 1639, 2764
BspMI ACCTGC 3 cut(s) 801, 1879, 2638
BspPI GGATC 7 cut(s) 239, 935, 948, 1110, 2037, 2112, 2710
BspQI GCTCTTC 1 cut(s) 293
BspT104I TTCGAA 1 cut(s) 2882
BspT107I GGYRCC 2 cut(s) 2918, 3035
BspTI CTTAAG 2 cut(s) 2119, 2129
BspTNI GGTCTC 3 cut(s) 974, 2037, 2536
BsrBI CCGCTC 1 cut(s) 2077
BsrDI GCAATG 3 cut(s) 1275, 1548, 2786
BsrFI RCCGGY 3 cut(s) 572, 651, 1700
BsrGI TGTACA 1 cut(s) 637
BsrI ACTGG 7 cut(s) 586, 996, 1453, 1976, 2172, 2227, 3041
BssAI RCCGGY 3 cut(s) 572, 651, 1700
BssECI CCNNGG 3 cut(s) 1428, 1429, 2698
BssNI GRCGYC 1 cut(s) 905
Bst2UI CCWGG 6 cut(s) 665, 1346, 1430, 1649, 2193, 2700
Bst4CI ACNGT 5 cut(s) 156, 1666, 1861, 2301, 2918
Bst6I CTCTTC 3 cut(s) 293, 332, 1133
BstACI GRCGYC 1 cut(s) 905
BstAFI CTTAAG 2 cut(s) 2119, 2129
BstAPI GCANNNNNTGC 1 cut(s) 1871
BstAUI TGTACA 1 cut(s) 637
BstBAI YACGTR 2 cut(s) 643, 2560
BstBI TTCGAA 1 cut(s) 2882
BstC8I GCNNGC 7 cut(s) 468, 798, 1457, 1572, 1630, 2259, 2992
BstENI CCTNNNNNAGG 1 cut(s) 1962
BstF5I GGATG 6 cut(s) 617, 744, 1369, 1936, 2812, 3023
BstMAI GTCTC 9 cut(s) 974, 1901, 1994, 2037, 2307, 2333, 2536, 2748, 2785
BstMWI GCNNNNNNNGC 8 cut(s) 495, 681, 1634, 1871, 1880, 2620, 2639, 2832
BstNI CCWGG 6 cut(s) 665, 1346, 1430, 1649, 2193, 2700
BstNSI RCATGY 3 cut(s) 1574, 1624, 2214
BstSCI CCNGG 7 cut(s) 663, 1344, 1428, 1647, 2191, 2698, 3031
BstSFI CTRYAG 5 cut(s) 464, 1635, 2056, 2464, 2760
BstSLI GKGCMC 5 cut(s) 78, 1393, 1429, 2769, 3040
BstV1I GCAGC 5 cut(s) 75, 133, 450, 2127, 3006
BstV2I GAAGAC 5 cut(s) 1057, 1786, 2102, 2612, 2723
BstX2I RGATCY 4 cut(s) 231, 940, 2042, 2887
BstYI RGATCY 4 cut(s) 231, 940, 2042, 2887
Bsu15I ATCGAT 1 cut(s) 88
BsuRI GGCC 6 cut(s) 76, 629, 1409, 2117, 2521, 2990
BsuTUI ATCGAT 1 cut(s) 88
BtgZI GCGATG 2 cut(s) 1184, 1453
BtrI CACGTC 1 cut(s) 718
BtsCI GGATG 6 cut(s) 617, 744, 1369, 1936, 2812, 3023
BtsI GCAGTG 3 cut(s) 1263, 2769, 2892
BtsIMutI CAGTG 5 cut(s) 1263, 2769, 2892, 2980, 3048
BveI ACCTGC 3 cut(s) 801, 1879, 2638
Cac8I GCNNGC 7 cut(s) 468, 798, 1457, 1572, 1630, 2259, 2992
CaiI CAGNNNCTG 1 cut(s) 2222
Cfr10I RCCGGY 3 cut(s) 572, 651, 1700
Cfr13I GGNCC 7 cut(s) 74, 75, 628, 790, 1293, 1698, 2627
ClaI ATCGAT 1 cut(s) 88
CseI GACGC 2 cut(s) 180, 894
CsiI ACCWGGT 1 cut(s) 663
CspAI ACCGGT 1 cut(s) 1700
CspCI CAANNNNNGTGG 2 cut(s) 1227, 1262
DraI TTTAAA 1 cut(s) 658
DraIII CACNNNGTG 1 cut(s) 3035
EaeI YGGCCR 1 cut(s) 2988
Eam1104I CTCTTC 3 cut(s) 293, 332, 1133
EarI CTCTTC 3 cut(s) 293, 332, 1133
Ecl136II GAGCTC 3 cut(s) 303, 1333, 2623
Eco24I GRGCYC 6 cut(s) 78, 305, 1335, 1917, 2625, 2879
Eco31I GGTCTC 3 cut(s) 974, 2037, 2536
Eco32I GATATC 3 cut(s) 2026, 2566, 2817
Eco47I GGWCC 4 cut(s) 790, 1293, 1698, 2627
Eco53kI GAGCTC 3 cut(s) 303, 1333, 2623
Eco57I CTGAAG 4 cut(s) 48, 237, 715, 2688
Eco72I CACGTG 1 cut(s) 2560
Eco88I CYCGRG 2 cut(s) 1334, 1898
EcoICRI GAGCTC 3 cut(s) 303, 1333, 2623
EcoNI CCTNNNNNAGG 1 cut(s) 1962
EcoO109I RGGNCCY 1 cut(s) 74
EcoRI GAATTC 3 cut(s) 280, 1789, 2080
EcoRII CCWGG 6 cut(s) 663, 1344, 1428, 1647, 2191, 2698
EcoRV GATATC 3 cut(s) 2026, 2566, 2817
EcoT22I ATGCAT 2 cut(s) 1445, 1622
EcoT38I GRGCYC 6 cut(s) 78, 305, 1335, 1917, 2625, 2879
Esp3I CGTCTC 3 cut(s) 1901, 2333, 2785
FalI AAGNNNNNCTT 2 cut(s) 1791, 1823
FaqI GGGAC 1 cut(s) 704
FauI CCCGC 1 cut(s) 2554
FbaI TGATCA 1 cut(s) 2383
Fnu4HI GCNGC 6 cut(s) 64, 147, 464, 2141, 2262, 2995
FokI GGATG 6 cut(s) 624, 731, 1376, 1943, 2819, 3010
FriOI GRGCYC 6 cut(s) 78, 305, 1335, 1917, 2625, 2879
Fsp4HI GCNGC 6 cut(s) 64, 147, 464, 2141, 2262, 2995
FspBI CTAG 7 cut(s) 929, 1010, 1418, 2009, 2345, 2615, 2727
GluI GCNGC 6 cut(s) 64, 147, 464, 2141, 2262, 2995
GsuI CTGGAG 2 cut(s) 1959, 2093
HaeIII GGCC 6 cut(s) 76, 629, 1409, 2117, 2521, 2990
HapII CCGG 5 cut(s) 573, 652, 1701, 2489, 3033
HgaI GACGC 2 cut(s) 180, 894
Hin1I GRCGYC 1 cut(s) 905
HincII GTYRAC 1 cut(s) 2674
HindII GTYRAC 1 cut(s) 2674
HinfI GANTC 9 cut(s) 68, 752, 1062, 1151, 1757, 2226, 2271, 2978, 3062
HpaI GTTAAC 1 cut(s) 2674
HpaII CCGG 5 cut(s) 573, 652, 1701, 2489, 3033
HphI GGTGA 7 cut(s) 16, 679, 1058, 1741, 2517, 2697, 3021
Hpy166II GTNNAC 9 cut(s) 481, 638, 640, 790, 1187, 2486, 2674, 2767, 2776
Hpy188III TCNNGA 9 cut(s) 521, 532, 929, 944, 1010, 1110, 1418, 2666, 2735
Hpy8I GTNNAC 9 cut(s) 481, 638, 640, 790, 1187, 2486, 2674, 2767, 2776
HpyAV CCTTC 9 cut(s) 641, 959, 1825, 1992, 2095, 2532, 2607, 2889, 3014
HpyCH4III ACNGT 5 cut(s) 156, 1666, 1861, 2301, 2918
HpyCH4IV ACGT 8 cut(s) 511, 642, 717, 956, 2559, 2658, 2778, 2955
HpyF10VI GCNNNNNNNGC 8 cut(s) 495, 681, 1634, 1871, 1880, 2620, 2639, 2832
HpySE526I ACGT 8 cut(s) 511, 642, 717, 956, 2559, 2658, 2778, 2955
Hsp92I GRCGYC 1 cut(s) 905
KpnI GGTACC 1 cut(s) 2922
Ksp22I TGATCA 1 cut(s) 2383
KspAI GTTAAC 1 cut(s) 2674
LguI GCTCTTC 1 cut(s) 293
LmnI GCTCC 6 cut(s) 308, 376, 1101, 1492, 2074, 2695
Lsp1109I GCAGC 5 cut(s) 75, 133, 450, 2127, 3006
MabI ACCWGGT 1 cut(s) 663
MaeI CTAG 7 cut(s) 929, 1010, 1418, 2009, 2345, 2615, 2727
MaeII ACGT 8 cut(s) 511, 642, 717, 956, 2559, 2658, 2778, 2955
MaeIII GTNAC 5 cut(s) 667, 1366, 1666, 2123, 2635
MbiI CCGCTC 1 cut(s) 2077
MfeI CAATTG 1 cut(s) 1935
MflI RGATCY 4 cut(s) 231, 940, 2042, 2887
MlsI TGGCCA 1 cut(s) 2990
MluNI TGGCCA 1 cut(s) 2990
MlyI GAGTC 2 cut(s) 1071, 3056
MmeI TCCRAC 3 cut(s) 66, 102, 2201
Mox20I TGGCCA 1 cut(s) 2990
Mph1103I ATGCAT 2 cut(s) 1445, 1622
MroXI GAANNNNTTC 2 cut(s) 155, 2496
MscI TGGCCA 1 cut(s) 2990
MslI CAYNNNNRTG 2 cut(s) 1221, 1268
Msp20I TGGCCA 1 cut(s) 2990
MspA1I CMGCKG 3 cut(s) 566, 1328, 2353
MspCI CTTAAG 2 cut(s) 2119, 2129
MspI CCGG 5 cut(s) 573, 652, 1701, 2489, 3033
MspR9I CCNGG 7 cut(s) 665, 1346, 1430, 1649, 2193, 2700, 3033
MunI CAATTG 1 cut(s) 1935
Mva1269I GAATGC 1 cut(s) 2298
MvaI CCWGG 6 cut(s) 665, 1346, 1430, 1649, 2193, 2700
MwoI GCNNNNNNNGC 8 cut(s) 495, 681, 1634, 1871, 1880, 2620, 2639, 2832
NciI CCSGG 1 cut(s) 3033
NlaIV GGNNCC 8 cut(s) 76, 942, 981, 1343, 1837, 2664, 2920, 3037
NmuCI GTSAC 2 cut(s) 667, 1666
NsiI ATGCAT 2 cut(s) 1445, 1622
NspI RCATGY 3 cut(s) 1574, 1624, 2214
NspV TTCGAA 1 cut(s) 2882
PaeI GCATGC 1 cut(s) 1574
PaeR7I CTCGAG 1 cut(s) 1334
PasI CCCWGGG 1 cut(s) 1429
PciI ACATGT 1 cut(s) 2210
PciSI GCTCTTC 1 cut(s) 293
PctI GAATGC 1 cut(s) 2298
PdmI GAANNNNTTC 2 cut(s) 155, 2496
PfeI GAWTC 7 cut(s) 68, 752, 1151, 1757, 2226, 2271, 2978
PflMI CCANNNNNTGG 2 cut(s) 2198, 2699
PfoI TCCNGGA 1 cut(s) 2191
PinAI ACCGGT 1 cut(s) 1700
PkrI GCNGC 6 cut(s) 65, 148, 465, 2142, 2263, 2996
PleI GAGTC 2 cut(s) 1070, 3056
PmaCI CACGTG 1 cut(s) 2560
PmlI CACGTG 1 cut(s) 2560
PpsI GAGTC 2 cut(s) 1070, 3056
Ppu21I YACGTR 2 cut(s) 643, 2560
PscI ACATGT 1 cut(s) 2210
PsiI TTATAA 1 cut(s) 1181
Psp124BI GAGCTC 3 cut(s) 305, 1335, 2625
Psp1406I AACGTT 1 cut(s) 2955
Psp6I CCWGG 6 cut(s) 663, 1344, 1428, 1647, 2191, 2698
PspCI CACGTG 1 cut(s) 2560
PspGI CCWGG 6 cut(s) 663, 1344, 1428, 1647, 2191, 2698
PspN4I GGNNCC 8 cut(s) 76, 942, 981, 1343, 1837, 2664, 2920, 3037
PspOMI GGGCCC 1 cut(s) 74
PspPI GGNCC 7 cut(s) 74, 75, 628, 790, 1293, 1698, 2627
PspXI VCTCGAGB 1 cut(s) 1334
PstI CTGCAG 3 cut(s) 468, 1639, 2764
PstNI CAGNNNCTG 1 cut(s) 2222
PsuI RGATCY 4 cut(s) 231, 940, 2042, 2887
PvuII CAGCTG 2 cut(s) 566, 1328
RseI CAYNNNNRTG 2 cut(s) 1221, 1268
SacI GAGCTC 3 cut(s) 305, 1335, 2625
SapI GCTCTTC 1 cut(s) 293
SatI GCNGC 6 cut(s) 64, 147, 464, 2141, 2262, 2995
Sau96I GGNCC 7 cut(s) 74, 75, 628, 790, 1293, 1698, 2627
ScaI AGTACT 3 cut(s) 503, 1566, 2860
SchI GAGTC 2 cut(s) 1071, 3056
ScrFI CCNGG 7 cut(s) 665, 1346, 1430, 1649, 2193, 2700, 3033
SexAI ACCWGGT 1 cut(s) 663
SfcI CTRYAG 5 cut(s) 464, 1635, 2056, 2464, 2760
Sfr274I CTCGAG 1 cut(s) 1334
SfuI TTCGAA 1 cut(s) 2882
SinI GGWCC 4 cut(s) 790, 1293, 1698, 2627
SlaI CTCGAG 1 cut(s) 1334
SmiMI CAYNNNNRTG 2 cut(s) 1221, 1268
SmlI CTYRAG 6 cut(s) 449, 484, 1334, 1560, 2119, 2129
SmoI CTYRAG 6 cut(s) 449, 484, 1334, 1560, 2119, 2129
SpeI ACTAGT 1 cut(s) 2344
SphI GCATGC 1 cut(s) 1574
SsiI CCGC 8 cut(s) 1307, 1710, 2077, 2261, 2353, 2374, 2547, 2908
SspI AATATT 3 cut(s) 421, 1948, 2589
SspMI CTAG 7 cut(s) 929, 1010, 1418, 2009, 2345, 2615, 2727
SstI GAGCTC 3 cut(s) 305, 1335, 2625
StyD4I CCNGG 7 cut(s) 663, 1344, 1428, 1647, 2191, 2698, 3031
TaaI ACNGT 5 cut(s) 156, 1666, 1861, 2301, 2918
TaiI ACGT 8 cut(s) 514, 645, 720, 959, 2562, 2661, 2781, 2958
TaqI TCGA 6 cut(s) 88, 1335, 1841, 2500, 2882, 2949
TatI WGTACW 6 cut(s) 252, 480, 501, 637, 1564, 2858
TauI GCSGC 1 cut(s) 2264
TfiI GAWTC 7 cut(s) 68, 752, 1151, 1757, 2226, 2271, 2978
TscAI CASTG 5 cut(s) 1270, 2769, 2899, 2980, 3048
TseFI GTSAC 2 cut(s) 667, 1666
TseI GCWGC 5 cut(s) 63, 146, 463, 2140, 2994
Tsp45I GTSAC 2 cut(s) 667, 1666
TspDTI ATGAA 6 cut(s) 1057, 1732, 1782, 1958, 2581, 2598
TspGWI ACGGA 3 cut(s) 222, 244, 1302
TspRI CASTG 5 cut(s) 1270, 2769, 2899, 2980, 3048
Van91I CCANNNNNTGG 2 cut(s) 2198, 2699
Vha464I CTTAAG 2 cut(s) 2119, 2129
VneI GTGCAC 1 cut(s) 2765
VpaK11BI GGWCC 4 cut(s) 790, 1293, 1698, 2627
XagI CCTNNNNNAGG 1 cut(s) 1962
XapI RAATTY 8 cut(s) 52, 280, 333, 472, 702, 1145, 1789, 2080
XbaI TCTAGA 3 cut(s) 928, 1009, 1417
XceI RCATGY 3 cut(s) 1574, 1624, 2214
XcmI CCANNNNNNNNNTGG 1 cut(s) 1924
XhoI CTCGAG 1 cut(s) 1334
XmnI GAANNNNTTC 2 cut(s) 155, 2496
XspI CTAG 7 cut(s) 929, 1010, 1418, 2009, 2345, 2615, 2727
ZrmI AGTACT 3 cut(s) 503, 1566, 2860
Zsp2I ATGCAT 2 cut(s) 1445, 1622
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.