MD07G1045800.v1.1

Retinoblastoma-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0003674
Physical Location & Seq
Forward (+)
3897047 .. 3905129
8083 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1045800.v1.1.491

Sequence Viewer

Length: 3072 bp
ATGGTGAAGATGGAAGATACTAAGCCTGAAGTTTCAGCTTCCAACAATTCAAATTCCGAGAGCAGCGATTCTGGTCCCGTCCAAGATCGATTTACTGATTTGTGCAAGAATGTATTGTCATTGGACGAGAGCTCTTACGCGGAAGCTGCAAAGCTGTTCAAAGAGACCAAACATCTGTTGATATCAAATGCGTCAGCTATTGGCAACGGAACACCTGAAGAAGCAGAACGATTCTGGTTTGCCTTTGTTTTGTACTCTGTAAAAACATTGAATGACAAGAATTCGGATAATTCACAGAAGAGTTCTGATGATAATGGATTTTCTCTCTTCCAAATTTTGAGAGCTGCAAAGCTGAACATTGTAGATTTTTTCAAGGAGCTTCCTCAGTTTGTTGTTAAAGCTGGTTCAATTTTAAGTAATATTTATGGTGTAGATTGGGAAAACAAACTTGAGGCAAAAGAGCTGCAGGCAAATTTTGTGTACTTGAGCTCGTTAAGCAAGTACTACAAACGTGCATATCGGGAGTTATTCTTGACAAGTGATACAACTGCCGACAAGCAGTCAACTGTTGTCAGTGGAACTGGTTATGTGTCAGAGTATCATCGTTTTGGATGGTTGCTGTTTTTGGCCCTTCGTGTACACGTATTCAGCCGATTTAAAGACCTGGTGACTTGCACAAACGGCTTGGTTGCAATATTGGGAATTTTAATAATTCATGTCCCTGTTCGCTTCAGAAAGTTCAGCATCCATGATTCAACACGCTTTGCAAGGAAAGGCAACAAGGGTGTGGACCTGCTTGCATGCCTCTGCAACACTTATAACACCTCAGAAGATGAGTTGAGAAAAACAATGGAAATGGCTAATGGTTTAATAGCAGAAATTTTGAAGAAGAAGCCCGTTTTGGCGTCTGATTATAAAACGGAAAATCTAGAGAATATGGATCCAGATGGTTTGACGTACTTTGAAGGTCTTATGGAGGAACCATCTTTGTTGTCCAGTTTAGAGATTCTAGAAAAAGATTACGATGATGCAATTAGGAATAAGGGAGAAGTTGATGAGAGAGTCTTCATCAATGAGGACGATAGCTTACTTGGTTCATGGAGCTTTTCAGGAGGATCCATGAGTGTAGCCGGTGTGAAGAGGAAATTTGATTCCATGGCCTCCCCAACAAAGACAATTACAAGTCCACTCTCTCCCCATCGCTCCCCTGCATCTCATGTCAGTGGTGGTGCTAATTCAACGATGGTGGCTACACCTGTGAGTACTGCAATGACAACTGCAAAGTGGCTTCGGACCTTTATTTCTCCGCTTCCGTCACAACCCTCAGCTGAGCTGGAGGGGTTCCTGGCATCGTGTGATAGGGATGTTACTACTGATGTGATACGTAGGGCACAAATTATATTGGAGGCCATATTTCCAAGTAGTGCATTGGGAGAGCGATGTGTAAGTGGCAGCCTGCAAGGTGCTAACCTCATGGACAACATCTGGTCAGAGCAACGAAGATTGGAAGCACTCAAACTTTATTATAGAGTTTTGGAGGCAATGTGTAGGGCAGAGGCTCAAGTACTGCATGCAACTAATTTGACCTCCTTATTAACCAATGAGAGGTTCCACAGATGCATGTTAGCCTGCTCTGGAGAGTTAGTCCTGGCAACACATAAGACTGTCACAATGTTGTTTCCAGCAGTATTAGTGAGGACGGGTATTACCGCTTTTGATCTTAGCAAGGTGATAGAGAGTTTCATTAGACATGAGGAATCTCTCCCAAGAGAATTAAGAAGACATCTGAATTCATTGGAAGAACGACTTTTGGAGAGCATGGTATGGGAGAAGGGTTCCTCGATGTATAATTCTTTAACTGTTGCAAGACCTGCCCTCTCAGCAGAAATAAATCGTCTTGGGTTATTAGCAGAGCCCATGCCATCTTTGGATGCAATTGCCATGAATATTAACTTCCCTTGTGGAGGGTTGCCTCCAGTACCTTCTTTACAGAAGCATGAGACTTCACCAGGCCAGAATGGAGATATCAGGTCTCCAAAGAGATCCTGCACAGATTATAGGAGTATATTGCTGGAGCGGAATTCCTTTACATCTCCTGTGAAGGATCGTCCTCTGGCATTAAGTAACCTTAAGTCAAAGCTGCCACCGCCTCCTTTACAATCTGCATTTGCCAGTCCAACTCGACCAAACCCAGGAGGTGGAGGGGAAACATGTGCAGAAACTGGAATTAGTATTTTCTTTAGCAAGATAATCAAGCTGGCAGCTGTCAGAATCAATGGTATGGTTGAAAGAATGCAACTGTCTCAGCAGATAAGAGAGAATGTTTATCGTCTCTTTCAACAAATACTTGTTCAGCGGACATCTCTCTTTTTTAACCGCCATATCGACCAGATTATCCTTTGTTGTTTTTATGGAGTTTCAAAGATTTCTCAACTAAATCTAACCTTTAGGGAAATCATATACAACTACAGAAAGCAACCACAATGTAAACCTCAAGTTTTTCGAAGCGTGTTTGTTGAGTGGCCTTCAGCACGCCGTAATGGGAGGCCCGCACAGGAACACGTGGATATCATAACATTTTACAATGAAATATTCATACCTTCTGTGAAGACCCTGCTAGTTGAGCTTGGACCTGCTGTTACAACAACTACAAGAGCTAACATGGTTCCTGAAGTTAACAATAATAATGATGCTCCATGCCCTGGATCACCTAAAGTATCTACTTTTCCAAGTCTTCCTGATATGTCACCAAAGAAAGTTTCTGCAGCGCACAATGTTTATGTCTCTCCATTGCGATCATCTAAGATGGATGCTTTGATCTCACATAGCTCAAAAAGCTATTATGCTTGTGTTGGGGAGAGTACTCATGCTTATCAGAGCCCTTCGAAAGATCTCACTGCCATCAACAACCGCTTGAACGGTACCCGAAAGCTCAGAGGTACTCTCAACTTCGACAGCGTTGATGTAGGCCTGGTCAGCGATTCGATGGTGGCCAACAGCCTGTACCTTCAAAATGGGAGTTGTGCAACCTCATCCAGTGCTCCAGTGAAAACTGAGCAACCTGACTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000082 GO:0000278 GO:0000280 GO:0000902 GO:0000904 GO:0001558 GO:0001708 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006275 GO:0006349 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007059 GO:0007127 GO:0007129 GO:0007275 GO:0008134 GO:0008150 GO:0008152 GO:0008356 GO:0009553 GO:0009555 GO:0009566 GO:0009567 GO:0009653 GO:0009791 GO:0009888 GO:0009889 GO:0009987 GO:0010016 GO:0010026 GO:0010052 GO:0010090 GO:0010103 GO:0010374 GO:0010377 GO:0010468 GO:0010556 GO:0010564 GO:0016043 GO:0019219 GO:0019222 GO:0019953 GO:0022402 GO:0022414 GO:0022619 GO:0030154 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032875 GO:0032989 GO:0033043 GO:0034641 GO:0040008 GO:0040029 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0044770 GO:0044772 GO:0044843 GO:0045132 GO:0045143 GO:0045165 GO:0046483 GO:0048229 GO:0048285 GO:0048366 GO:0048367 GO:0048468 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051052 GO:0051128 GO:0051171 GO:0051239 GO:0051276 GO:0051301 GO:0051321 GO:0051704 GO:0051726 GO:0051783 GO:0055046 GO:0060255 GO:0061982 GO:0065007 GO:0070192 GO:0071514 GO:0071704 GO:0071840 GO:0080090 GO:0090329 GO:0090558 GO:0090626 GO:0090627 GO:0090698 GO:0097159 GO:0098813 GO:0099402 GO:0140013 GO:1901360 GO:1901363 GO:1903046 GO:1903047 GO:1903866 GO:2000036 GO:2000112 GO:2000653
KEGG Pathways
Metabolic & Signaling
ko04110 ko04218 ko04350 ko05165 ko05203 map04110 map04218 map04350 map05165 map05203
Pfam Domains
Protein Families
DUF3452 RB_A RB_B Rb_C

Protein Analysis

1024

Amino Acids

113.6

Weight (kDa)

8.25

Isoelectric Point (pI)

52.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3452 PF11934 100 - 239 1e-35 Domain of unknown function (DUF3452)
RB_A PF01858 418 - 619 9.9e-63 Retinoblastoma-associated protein A domain
RB_B PF01857 743 - 870 1.2e-42 Retinoblastoma-associated protein B domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 819, 915
Acc36I ACCTGC 3 cut(s) 801, 1879, 2641
Acc65I GGTACC 1 cut(s) 2921
AccB1I GGYRCC 1 cut(s) 2921
AccB7I CCANNNNNTGG 2 cut(s) 2198, 2702
AccBSI CCGCTC 1 cut(s) 2077
AccII CGCG 1 cut(s) 140
AciI CCGC 9 cut(s) 140, 1307, 1710, 2077, 2147, 2356, 2377, 2550, 2911
AclWI GGATC 7 cut(s) 935, 948, 1110, 1123, 2037, 2112, 2713
AcoI YGGCCR 1 cut(s) 2991
AcsI RAATTY 9 cut(s) 52, 280, 333, 472, 702, 879, 1145, 1789, 2080
AcuI CTGAAG 5 cut(s) 48, 237, 715, 2511, 2691
AcvI CACGTG 1 cut(s) 2563
AcyI GRCGYC 1 cut(s) 905
AfiI CCNNNNNNNGG 5 cut(s) 1605, 1964, 2192, 2198, 2702
AflII CTTAAG 1 cut(s) 2129
AflIII ACRYGT 3 cut(s) 640, 2210, 2560
AhdI GACNNNNNGTC 1 cut(s) 559
AjnI CCWGG 7 cut(s) 663, 1344, 1647, 2008, 2191, 2701, 2970
AjuI GAANNNNNNNTTGG 6 cut(s) 884, 916, 1592, 1624, 2743, 2775
Alw21I GWGCWC 3 cut(s) 134, 491, 3043
Alw26I GTCTC 6 cut(s) 158, 1994, 2037, 2307, 2336, 2788
AlwI GGATC 7 cut(s) 935, 948, 1110, 1123, 2037, 2112, 2713
AlwNI CAGNNNCTG 1 cut(s) 2222
AoxI GGCC 8 cut(s) 627, 1158, 1407, 2011, 2522, 2546, 2968, 2991
ApeKI GCWGC 8 cut(s) 63, 146, 344, 463, 1452, 2140, 2261, 2765
ApoI RAATTY 9 cut(s) 52, 280, 333, 472, 702, 879, 1145, 1789, 2080
Asp718I GGTACC 1 cut(s) 2921
AspLEI GCGC 1 cut(s) 2770
AspS9I GGNCC 6 cut(s) 74, 628, 790, 1293, 2547, 2630
AsuHPI GGTGA 6 cut(s) 16, 679, 1741, 1998, 2700, 2739
AsuII TTCGAA 2 cut(s) 2503, 2885
AvaII GGWCC 4 cut(s) 74, 790, 1293, 2630
BaeGI GKGCMC 1 cut(s) 1393
BalI TGGCCA 1 cut(s) 2993
BamHI GGATCC 2 cut(s) 940, 1115
BanI GGYRCC 1 cut(s) 2921
BanII GRGCYC 4 cut(s) 134, 491, 1917, 2882
BbrPI CACGTG 1 cut(s) 2563
BbsI GAAGAC 4 cut(s) 1057, 1786, 2615, 2726
Bbv12I GWGCWC 3 cut(s) 134, 491, 3043
BbvCI CCTCAGC 1 cut(s) 1324
BbvI GCAGC 8 cut(s) 75, 133, 331, 450, 1464, 2127, 2273, 2777
BceAI ACGGC 2 cut(s) 697, 2520
BciT130I CCWGG 7 cut(s) 665, 1346, 1649, 2010, 2193, 2703, 2972
BcoDI GTCTC 6 cut(s) 158, 1994, 2037, 2307, 2336, 2788
BfaI CTAG 3 cut(s) 929, 1010, 2618
BfmI CTRYAG 3 cut(s) 464, 2467, 2763
BfrI CTTAAG 1 cut(s) 2129
BfuAI ACCTGC 3 cut(s) 801, 1879, 2641
BglII AGATCT 1 cut(s) 2890
BisI GCNGC 8 cut(s) 64, 147, 345, 464, 1453, 2141, 2262, 2766
BlpI GCTNAGC 1 cut(s) 1329
BlsI GCNGC 8 cut(s) 65, 148, 346, 465, 1454, 2142, 2263, 2767
BmcAI AGTACT 4 cut(s) 503, 1264, 1566, 2863
Bme1390I CCNGG 7 cut(s) 665, 1346, 1649, 2010, 2193, 2703, 2972
Bme18I GGWCC 4 cut(s) 74, 790, 1293, 2630
BmeRI GACNNNNNGTC 1 cut(s) 559
BmgT120I GGNCC 6 cut(s) 74, 628, 790, 1293, 2547, 2630
BmiI GGNNCC 9 cut(s) 76, 942, 981, 1117, 1343, 1610, 1837, 2667, 2923
BmrFI CCNGG 7 cut(s) 665, 1346, 1649, 2010, 2193, 2703, 2972
BmsI GCATC 8 cut(s) 753, 1018, 1220, 1358, 1607, 1921, 2680, 2800
BpiI GAAGAC 4 cut(s) 1057, 1786, 2615, 2726
BplI GAGNNNNNCTC 2 cut(s) 2928, 2960
BpmI CTGGAG 5 cut(s) 1355, 1656, 1959, 2093, 3027
Bpu10I CCTNAGC 1 cut(s) 1324
Bpu1102I GCTNAGC 1 cut(s) 1329
Bpu14I TTCGAA 2 cut(s) 2503, 2885
BpuEI CTTGAG 4 cut(s) 470, 505, 1545, 2478
Bsa29I ATCGAT 1 cut(s) 88
BsaAI YACGTR 3 cut(s) 643, 1385, 2563
BsaHI GRCGYC 1 cut(s) 905
BsaI GGTCTC 2 cut(s) 158, 2037
BsaJI CCNNGG 3 cut(s) 1155, 2191, 2701
BsaXI ACNNNNNCTCC 4 cut(s) 1529, 1559, 1629, 1659
Bsc4I CCNNNNNNNGG 5 cut(s) 1605, 1964, 2192, 2198, 2702
Bse118I RCCGGY 1 cut(s) 1130
Bse1I ACTGG 7 cut(s) 586, 996, 1976, 2172, 2227, 3036, 3044
Bse3DI GCAATG 3 cut(s) 1275, 1548, 2789
BseBI CCWGG 7 cut(s) 665, 1346, 1649, 2010, 2193, 2703, 2972
BseCI ATCGAT 1 cut(s) 88
BseDI CCNNGG 3 cut(s) 1155, 2191, 2701
BseGI GGATG 6 cut(s) 617, 744, 1369, 1936, 2815, 3032
BseLI CCNNNNNNNGG 5 cut(s) 1605, 1964, 2192, 2198, 2702
BseMI GCAATG 3 cut(s) 1275, 1548, 2789
BseMII CTCAG 8 cut(s) 398, 840, 1320, 1338, 1893, 2318, 2947, 3045
BseNI ACTGG 7 cut(s) 586, 996, 1976, 2172, 2227, 3036, 3044
BseSI GKGCMC 1 cut(s) 1393
BseXI GCAGC 8 cut(s) 75, 133, 331, 450, 1464, 2127, 2273, 2777
BsgI GTGCAG 2 cut(s) 2032, 2235
Bsh1236I CGCG 1 cut(s) 140
BshFI GGCC 8 cut(s) 629, 1160, 1409, 2013, 2524, 2548, 2970, 2993
BshNI GGYRCC 1 cut(s) 2921
BshVI ATCGAT 1 cut(s) 88
BsiHKAI GWGCWC 3 cut(s) 134, 491, 3043
BsiSI CCGG 1 cut(s) 1131
BslFI GGGAC 2 cut(s) 60, 704
BslI CCNNNNNNNGG 5 cut(s) 1605, 1964, 2192, 2198, 2702
BsmAI GTCTC 6 cut(s) 158, 1994, 2037, 2307, 2336, 2788
BsmBI CGTCTC 1 cut(s) 2336
BsmFI GGGAC 2 cut(s) 60, 704
BsmI GAATGC 1 cut(s) 2298
BsnI GGCC 8 cut(s) 629, 1160, 1409, 2013, 2524, 2548, 2970, 2993
Bso31I GGTCTC 2 cut(s) 158, 2037
Bsp119I TTCGAA 2 cut(s) 2503, 2885
Bsp1286I GDGCHC 6 cut(s) 134, 491, 1393, 1917, 2882, 3043
Bsp1407I TGTACA 1 cut(s) 637
Bsp1720I GCTNAGC 1 cut(s) 1329
Bsp19I CCATGG 1 cut(s) 1155
BspACI CCGC 9 cut(s) 140, 1307, 1710, 2077, 2147, 2356, 2377, 2550, 2911
BspANI GGCC 8 cut(s) 629, 1160, 1409, 2013, 2524, 2548, 2970, 2993
BspCNI CTCAG 8 cut(s) 397, 839, 1321, 1337, 1892, 2317, 2946, 3046
BspDI ATCGAT 1 cut(s) 88
BspFNI CGCG 1 cut(s) 140
BspLI GGNNCC 9 cut(s) 76, 942, 981, 1117, 1343, 1610, 1837, 2667, 2923
BspMAI CTGCAG 2 cut(s) 468, 2767
BspMI ACCTGC 3 cut(s) 801, 1879, 2641
BspPI GGATC 7 cut(s) 935, 948, 1110, 1123, 2037, 2112, 2713
BspT104I TTCGAA 2 cut(s) 2503, 2885
BspT107I GGYRCC 1 cut(s) 2921
BspTI CTTAAG 1 cut(s) 2129
BspTNI GGTCTC 2 cut(s) 158, 2037
BsrBI CCGCTC 1 cut(s) 2077
BsrDI GCAATG 3 cut(s) 1275, 1548, 2789
BsrFI RCCGGY 1 cut(s) 1130
BsrGI TGTACA 1 cut(s) 637
BsrI ACTGG 7 cut(s) 586, 996, 1976, 2172, 2227, 3036, 3044
BssAI RCCGGY 1 cut(s) 1130
BssECI CCNNGG 3 cut(s) 1155, 2191, 2701
BssNI GRCGYC 1 cut(s) 905
BssT1I CCWWGG 1 cut(s) 1155
Bst2UI CCWGG 7 cut(s) 665, 1346, 1649, 2010, 2193, 2703, 2972
Bst4CI ACNGT 5 cut(s) 568, 1666, 1861, 2301, 2921
Bst6I CTCTTC 3 cut(s) 293, 332, 1133
BstACI GRCGYC 1 cut(s) 905
BstAFI CTTAAG 1 cut(s) 2129
BstAPI GCANNNNNTGC 1 cut(s) 1871
BstAUI TGTACA 1 cut(s) 637
BstBAI YACGTR 3 cut(s) 643, 1385, 2563
BstBI TTCGAA 2 cut(s) 2503, 2885
BstC8I GCNNGC 9 cut(s) 468, 798, 802, 1457, 1572, 1630, 2259, 2533, 2550
BstDSI CCRYGG 1 cut(s) 1155
BstENI CCTNNNNNAGG 1 cut(s) 1962
BstF5I GGATG 6 cut(s) 617, 744, 1369, 1936, 2815, 3032
BstFNI CGCG 1 cut(s) 140
BstHHI GCGC 1 cut(s) 2770
BstMAI GTCTC 6 cut(s) 158, 1994, 2037, 2307, 2336, 2788
BstMWI GCNNNNNNNGC 9 cut(s) 146, 495, 681, 1871, 1880, 2146, 2623, 2835, 2976
BstNI CCWGG 7 cut(s) 665, 1346, 1649, 2010, 2193, 2703, 2972
BstNSI RCATGY 4 cut(s) 804, 1574, 1624, 2214
BstSCI CCNGG 7 cut(s) 663, 1344, 1647, 2008, 2191, 2701, 2970
BstSFI CTRYAG 3 cut(s) 464, 2467, 2763
BstSLI GKGCMC 1 cut(s) 1393
BstSNI TACGTA 1 cut(s) 1385
BstUI CGCG 1 cut(s) 140
BstV1I GCAGC 8 cut(s) 75, 133, 331, 450, 1464, 2127, 2273, 2777
BstV2I GAAGAC 4 cut(s) 1057, 1786, 2615, 2726
BstX2I RGATCY 4 cut(s) 940, 1115, 2042, 2890
BstYI RGATCY 4 cut(s) 940, 1115, 2042, 2890
Bsu15I ATCGAT 1 cut(s) 88
BsuRI GGCC 8 cut(s) 629, 1160, 1409, 2013, 2524, 2548, 2970, 2993
BsuTUI ATCGAT 1 cut(s) 88
BtgI CCRYGG 1 cut(s) 1155
BtgZI GCGATG 2 cut(s) 1184, 1453
BtsCI GGATG 6 cut(s) 617, 744, 1369, 1936, 2815, 3032
BtsI GCAGTG 1 cut(s) 2895
BtsIMutI CAGTG 5 cut(s) 580, 1228, 2895, 3043, 3051
BveI ACCTGC 3 cut(s) 801, 1879, 2641
Cac8I GCNNGC 9 cut(s) 468, 798, 802, 1457, 1572, 1630, 2259, 2533, 2550
CaiI CAGNNNCTG 1 cut(s) 2222
CfoI GCGC 1 cut(s) 2770
Cfr10I RCCGGY 1 cut(s) 1130
Cfr13I GGNCC 6 cut(s) 74, 628, 790, 1293, 2547, 2630
ClaI ATCGAT 1 cut(s) 88
CseI GACGC 2 cut(s) 180, 894
CsiI ACCWGGT 1 cut(s) 663
CspCI CAANNNNNGTGG 2 cut(s) 1227, 1262
DraI TTTAAA 1 cut(s) 658
DriI GACNNNNNGTC 1 cut(s) 559
EaeI YGGCCR 1 cut(s) 2991
Eam1104I CTCTTC 3 cut(s) 293, 332, 1133
Eam1105I GACNNNNNGTC 1 cut(s) 559
EarI CTCTTC 3 cut(s) 293, 332, 1133
Ecl136II GAGCTC 2 cut(s) 132, 489
Eco105I TACGTA 1 cut(s) 1385
Eco130I CCWWGG 1 cut(s) 1155
Eco147I AGGCCT 1 cut(s) 2970
Eco24I GRGCYC 4 cut(s) 134, 491, 1917, 2882
Eco31I GGTCTC 2 cut(s) 158, 2037
Eco32I GATATC 3 cut(s) 183, 2026, 2569
Eco47I GGWCC 4 cut(s) 74, 790, 1293, 2630
Eco53kI GAGCTC 2 cut(s) 132, 489
Eco57I CTGAAG 5 cut(s) 48, 237, 715, 2511, 2691
Eco72I CACGTG 1 cut(s) 2563
EcoICRI GAGCTC 2 cut(s) 132, 489
EcoNI CCTNNNNNAGG 1 cut(s) 1962
EcoRI GAATTC 3 cut(s) 280, 1789, 2080
EcoRII CCWGG 7 cut(s) 663, 1344, 1647, 2008, 2191, 2701, 2970
EcoRV GATATC 3 cut(s) 183, 2026, 2569
EcoT14I CCWWGG 1 cut(s) 1155
EcoT22I ATGCAT 1 cut(s) 1622
EcoT38I GRGCYC 4 cut(s) 134, 491, 1917, 2882
ErhI CCWWGG 1 cut(s) 1155
Esp3I CGTCTC 1 cut(s) 2336
FalI AAGNNNNNCTT 2 cut(s) 1791, 1823
FaqI GGGAC 2 cut(s) 60, 704
FauI CCCGC 1 cut(s) 2557
Fnu4HI GCNGC 8 cut(s) 64, 147, 345, 464, 1453, 2141, 2262, 2766
FokI GGATG 6 cut(s) 624, 731, 1376, 1943, 2822, 3019
FriOI GRGCYC 4 cut(s) 134, 491, 1917, 2882
Fsp4HI GCNGC 8 cut(s) 64, 147, 345, 464, 1453, 2141, 2262, 2766
FspBI CTAG 3 cut(s) 929, 1010, 2618
GlaI GCGC 1 cut(s) 2769
GluI GCNGC 8 cut(s) 64, 147, 345, 464, 1453, 2141, 2262, 2766
GsuI CTGGAG 5 cut(s) 1355, 1656, 1959, 2093, 3027
HaeIII GGCC 8 cut(s) 629, 1160, 1409, 2013, 2524, 2548, 2970, 2993
HapII CCGG 1 cut(s) 1131
HgaI GACGC 2 cut(s) 180, 894
HhaI GCGC 1 cut(s) 2770
Hin1I GRCGYC 1 cut(s) 905
Hin6I GCGC 1 cut(s) 2768
HinP1I GCGC 1 cut(s) 2768
HincII GTYRAC 2 cut(s) 564, 2677
HindII GTYRAC 2 cut(s) 564, 2677
HpaI GTTAAC 1 cut(s) 2677
HpaII CCGG 1 cut(s) 1131
HphI GGTGA 6 cut(s) 16, 679, 1741, 1998, 2700, 2739
Hpy166II GTNNAC 8 cut(s) 481, 564, 638, 640, 790, 1187, 2489, 2677
Hpy8I GTNNAC 8 cut(s) 481, 564, 638, 640, 790, 1187, 2489, 2677
HpyAV CCTTC 9 cut(s) 641, 959, 1825, 1992, 2095, 2535, 2610, 2892, 3017
HpyCH4III ACNGT 5 cut(s) 568, 1666, 1861, 2301, 2921
HpyCH4IV ACGT 5 cut(s) 511, 642, 956, 1384, 2562
HpyF10VI GCNNNNNNNGC 9 cut(s) 146, 495, 681, 1871, 1880, 2146, 2623, 2835, 2976
HpySE526I ACGT 5 cut(s) 511, 642, 956, 1384, 2562
Hsp92I GRCGYC 1 cut(s) 905
HspAI GCGC 1 cut(s) 2768
KpnI GGTACC 1 cut(s) 2925
KspAI GTTAAC 1 cut(s) 2677
LmnI GCTCC 6 cut(s) 376, 1101, 1208, 2074, 2698, 3046
Lsp1109I GCAGC 8 cut(s) 75, 133, 331, 450, 1464, 2127, 2273, 2777
LweI GCATC 8 cut(s) 753, 1018, 1220, 1358, 1607, 1921, 2680, 2800
MabI ACCWGGT 1 cut(s) 663
MaeI CTAG 3 cut(s) 929, 1010, 2618
MaeII ACGT 5 cut(s) 511, 642, 956, 1384, 2562
MaeIII GTNAC 7 cut(s) 667, 1314, 1366, 1666, 2123, 2638, 2745
MbiI CCGCTC 1 cut(s) 2077
MfeI CAATTG 1 cut(s) 1935
MflI RGATCY 4 cut(s) 940, 1115, 2042, 2890
MhlI GDGCHC 6 cut(s) 134, 491, 1393, 1917, 2882, 3043
MlsI TGGCCA 1 cut(s) 2993
MluNI TGGCCA 1 cut(s) 2993
MlyI GAGTC 2 cut(s) 1071, 3059
MmeI TCCRAC 2 cut(s) 66, 2201
Mox20I TGGCCA 1 cut(s) 2993
Mph1103I ATGCAT 1 cut(s) 1622
MscI TGGCCA 1 cut(s) 2993
MslI CAYNNNNRTG 1 cut(s) 1221
Msp20I TGGCCA 1 cut(s) 2993
MspA1I CMGCKG 3 cut(s) 1328, 2264, 2356
MspCI CTTAAG 1 cut(s) 2129
MspI CCGG 1 cut(s) 1131
MspR9I CCNGG 7 cut(s) 665, 1346, 1649, 2010, 2193, 2703, 2972
MunI CAATTG 1 cut(s) 1935
Mva1269I GAATGC 1 cut(s) 2298
MvaI CCWGG 7 cut(s) 665, 1346, 1649, 2010, 2193, 2703, 2972
MvnI CGCG 1 cut(s) 140
MwoI GCNNNNNNNGC 9 cut(s) 146, 495, 681, 1871, 1880, 2146, 2623, 2835, 2976
NcoI CCATGG 1 cut(s) 1155
NlaIV GGNNCC 9 cut(s) 76, 942, 981, 1117, 1343, 1610, 1837, 2667, 2923
NmuCI GTSAC 4 cut(s) 667, 1314, 1666, 2745
NsiI ATGCAT 1 cut(s) 1622
NspI RCATGY 4 cut(s) 804, 1574, 1624, 2214
NspV TTCGAA 2 cut(s) 2503, 2885
PaeI GCATGC 2 cut(s) 804, 1574
PceI AGGCCT 1 cut(s) 2970
PciI ACATGT 1 cut(s) 2210
PctI GAATGC 1 cut(s) 2298
PfeI GAWTC 8 cut(s) 68, 231, 752, 1006, 1151, 1757, 2271, 2981
PflMI CCANNNNNTGG 2 cut(s) 2198, 2702
PkrI GCNGC 8 cut(s) 65, 148, 346, 465, 1454, 2142, 2263, 2767
PleI GAGTC 2 cut(s) 1070, 3059
PmaCI CACGTG 1 cut(s) 2563
PmlI CACGTG 1 cut(s) 2563
PpsI GAGTC 2 cut(s) 1070, 3059
Ppu21I YACGTR 3 cut(s) 643, 1385, 2563
PscI ACATGT 1 cut(s) 2210
PsiI TTATAA 2 cut(s) 819, 915
Psp124BI GAGCTC 2 cut(s) 134, 491
Psp6I CCWGG 7 cut(s) 663, 1344, 1647, 2008, 2191, 2701, 2970
PspCI CACGTG 1 cut(s) 2563
PspGI CCWGG 7 cut(s) 663, 1344, 1647, 2008, 2191, 2701, 2970
PspN4I GGNNCC 9 cut(s) 76, 942, 981, 1117, 1343, 1610, 1837, 2667, 2923
PspPI GGNCC 6 cut(s) 74, 628, 790, 1293, 2547, 2630
PstI CTGCAG 2 cut(s) 468, 2767
PstNI CAGNNNCTG 1 cut(s) 2222
PsuI RGATCY 4 cut(s) 940, 1115, 2042, 2890
PvuII CAGCTG 2 cut(s) 1328, 2264
RseI CAYNNNNRTG 1 cut(s) 1221
SacI GAGCTC 2 cut(s) 134, 491
SatI GCNGC 8 cut(s) 64, 147, 345, 464, 1453, 2141, 2262, 2766
Sau96I GGNCC 6 cut(s) 74, 628, 790, 1293, 2547, 2630
ScaI AGTACT 4 cut(s) 503, 1264, 1566, 2863
SchI GAGTC 2 cut(s) 1071, 3059
ScrFI CCNGG 7 cut(s) 665, 1346, 1649, 2010, 2193, 2703, 2972
SduI GDGCHC 6 cut(s) 134, 491, 1393, 1917, 2882, 3043
SexAI ACCWGGT 1 cut(s) 663
SfaNI GCATC 8 cut(s) 753, 1018, 1220, 1358, 1607, 1921, 2680, 2800
SfcI CTRYAG 3 cut(s) 464, 2467, 2763
SfuI TTCGAA 2 cut(s) 2503, 2885
SinI GGWCC 4 cut(s) 74, 790, 1293, 2630
SmiMI CAYNNNNRTG 1 cut(s) 1221
SmlI CTYRAG 5 cut(s) 449, 484, 1560, 2129, 2493
SmoI CTYRAG 5 cut(s) 449, 484, 1560, 2129, 2493
SnaBI TACGTA 1 cut(s) 1385
SphI GCATGC 2 cut(s) 804, 1574
SseBI AGGCCT 1 cut(s) 2970
SsiI CCGC 9 cut(s) 140, 1307, 1710, 2077, 2147, 2356, 2377, 2550, 2911
SspI AATATT 4 cut(s) 421, 696, 1948, 2592
SspMI CTAG 3 cut(s) 929, 1010, 2618
SstI GAGCTC 2 cut(s) 134, 491
StuI AGGCCT 1 cut(s) 2970
StyD4I CCNGG 7 cut(s) 663, 1344, 1647, 2008, 2191, 2701, 2970
StyI CCWWGG 1 cut(s) 1155
TaaI ACNGT 5 cut(s) 568, 1666, 1861, 2301, 2921
TaiI ACGT 5 cut(s) 514, 645, 959, 1387, 2565
TaqI TCGA 8 cut(s) 88, 1841, 2182, 2385, 2503, 2885, 2952, 2984
TatI WGTACW 7 cut(s) 252, 480, 501, 637, 1262, 1564, 2861
TfiI GAWTC 8 cut(s) 68, 231, 752, 1006, 1151, 1757, 2271, 2981
TscAI CASTG 5 cut(s) 580, 1228, 2902, 3043, 3051
TseFI GTSAC 4 cut(s) 667, 1314, 1666, 2745
TseI GCWGC 8 cut(s) 63, 146, 344, 463, 1452, 2140, 2261, 2765
Tsp45I GTSAC 4 cut(s) 667, 1314, 1666, 2745
TspDTI ATGAA 8 cut(s) 704, 1057, 1086, 1732, 1782, 1958, 2584, 2601
TspGWI ACGGA 3 cut(s) 222, 935, 1302
TspRI CASTG 5 cut(s) 580, 1228, 2902, 3043, 3051
Van91I CCANNNNNTGG 2 cut(s) 2198, 2702
Vha464I CTTAAG 1 cut(s) 2129
VpaK11BI GGWCC 4 cut(s) 74, 790, 1293, 2630
XagI CCTNNNNNAGG 1 cut(s) 1962
XapI RAATTY 9 cut(s) 52, 280, 333, 472, 702, 879, 1145, 1789, 2080
XbaI TCTAGA 2 cut(s) 928, 1009
XceI RCATGY 4 cut(s) 804, 1574, 1624, 2214
XcmI CCANNNNNNNNNTGG 1 cut(s) 1924
XspI CTAG 3 cut(s) 929, 1010, 2618
ZrmI AGTACT 4 cut(s) 503, 1264, 1566, 2863
Zsp2I ATGCAT 1 cut(s) 1622
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.