FvH4_7g05560

Belongs to the membrane-bound acyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0004143
Physical Location & Seq
Forward (+)
5841173 .. 5853035
11863 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g05560.t3

Sequence Viewer

Length: 1596 bp
ATGGATTTGAAACGCAGCAATACTAGTAGGGGTAGTAAGATGAGAGAGCTGCCATTTCTGGTTCTCTACGCCGCCGCTTTCTACGCCTTCATCATCCGTCGATCGCTTCAACTGTCTCGCGATCATTATGCTGAACTGTTCGGATTGCGTCCTGGGTGGCTCACACCTCAATGCCTCAATGATGTGTCTGATGCTCAATGGAGAAACTTTCGAGCAAATTTACCTGTTCTCACCATTGTTTTCGCAATATTTACGACAATCGCTCAGTTGTTGCGGGCATATTTTCATTTGAAAGGCAGAGGGATGTCCATTGTTTGGCTTTTGATTTCCTTGACATACCTGACGTATCTGCATGGAGCCTGCATCATATTTATCCTCTCAATCGCTTCAGTGAATTTTCTTTTGGTAAAGATATTTGCTCGGACGAAGTACTTCTCTTTGGTATTCTGGAGTTTCAATATATTCTTTCTTCTCTGCAATCGTATTTATGAAGGATACTCATTTTCCTCATTTGGGGAGCACTGGGCATTATTGGACAACTTTCGGGGCACCTTTAGATGGCAAATATGCTTCAACTTCGTTGTTCTGCGCATGGTAAGCTTTGGTTATGATTACCATTGGGCTCTTCAAGATTCTCGTTTCGACCATGAGAAACATATACAGCGTTGCACTGTCTGTAGAGCCGGGAAAACTTGTTACCATTTTTTACAGGAGAGAGGTGTCCAGAGCGACAAGTTTGCCTTTGTCACATACCTTTCTTATCTGGCATATGCCCCTCTTTATCTTGCTGGGCCAATTCTAAGCTTCAATGCCTTTGCTTCACAGCTGGACATTCCTCAAAATAACTATTCAGTTAGAGACACTACTTGGTACGGTTTGCGTTGGCTGTTAAGTCTTCTTCTGATGGAACTAATGACACATCTCTTTTACTACAATGCCTTTGCAATCAGTGGTATATGGAAGCAGTTAAATCCTGTAGACGTGTTTATTATTGGATATGGGGTTTTAATCTTCATGTGGCTAAAGTTTTTTCTAGTATGGCGCTATTTCCGATTCTGGTCACTGATCTGTGGCATTGAGGTCCCTGAGAATATGCCAAAGTGTGTAAACAATTGCTACAACTTGGAAAGCTTTTGGAAGAACTGGCATGCTTCCTACAACAAATGGCTAGTAAGGTATTTGTACATTCCTCTTGGCGGCTCTCAGAGAAAGCTTCTCAATGTGTGGGTTGTATTCACATTCGTTGCCATTTGGCATGATTTAGAGTGGAAGCTTCTTTCATGGGCATGGCTGACATGTTTATTCTTTATTCCGGAAATAATCTTCAAATCAGCAGCAAATGCATTTCAGGCAAAGACTGCTCTTGGGGAGTTTATTGTTCGTGAATTCAGTGCGGCTGCTGGGGCTATCACAATCACTTGCCTCATGGTTGCAAACCTTGTTGGTTTTGTGATCGGACCATCTGGGATTAGTTGGTTGGTTTCTCAATTCCTTAGCAGAGAAGGATTACCGGTCTTGGCTGGCATGCTTTTAACATTTTATGTTGGTACAAAGCTTATGTTCCACATCCGTGATGCCAAACAAAGTACCCGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MBOAT

Protein Analysis

532

Amino Acids

62.12

Weight (kDa)

9.45

Isoelectric Point (pI)

39.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MBOAT PF03062 55 - 341 5.5e-40 MBOAT, membrane-bound O-acyltransferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 590
AccB1I GGYRCC 1 cut(s) 548
AccB7I CCANNNNNTGG 1 cut(s) 315
AccI GTMKAC 1 cut(s) 978
AccII CGCG 1 cut(s) 120
AccIII TCCGGA 1 cut(s) 1312
AciI CCGC 6 cut(s) 72, 75, 274, 1197, 1394, 1591
AcsI RAATTY 3 cut(s) 217, 394, 1385
AcuI CTGAAG 1 cut(s) 372
AfaI GTAC 5 cut(s) 431, 872, 1184, 1549, 1588
AfiI CCNNNNNNNGG 4 cut(s) 315, 513, 558, 1196
AflIII ACRYGT 2 cut(s) 981, 1295
AgeI ACCGGT 1 cut(s) 1510
AgsI TTSAA 8 cut(s) 10, 110, 292, 457, 574, 629, 808, 1327
AhlI ACTAGT 1 cut(s) 23
AjiI CACGTC 1 cut(s) 982
AjnI CCWGG 1 cut(s) 151
AjuI GAANNNNNNNTTGG 2 cut(s) 386, 418
AleI CACNNNNGTG 1 cut(s) 1569
AluBI AGCT 8 cut(s) 49, 600, 804, 826, 1131, 1213, 1273, 1555
AluI AGCT 8 cut(s) 49, 600, 804, 826, 1131, 1213, 1273, 1555
Alw21I GWGCWC 1 cut(s) 522
Alw26I GTCTC 2 cut(s) 120, 852
Aor13HI TCCGGA 1 cut(s) 1312
AoxI GGCC 1 cut(s) 791
ApeKI GCWGC 4 cut(s) 15, 49, 1334, 1397
ApoI RAATTY 3 cut(s) 217, 394, 1385
AsiGI ACCGGT 1 cut(s) 1510
Asp700I GAANNNNTTC 1 cut(s) 431
AspLEI GCGC 2 cut(s) 591, 1044
AspS9I GGNCC 3 cut(s) 791, 1081, 1457
AsuC2I CCSGG 1 cut(s) 685
AsuHPI GGTGA 1 cut(s) 223
AvaII GGWCC 2 cut(s) 1081, 1457
BaeGI GKGCMC 1 cut(s) 551
BanI GGYRCC 1 cut(s) 548
BanII GRGCYC 1 cut(s) 625
BbsI GAAGAC 1 cut(s) 887
Bbv12I GWGCWC 1 cut(s) 522
BbvI GCAGC 4 cut(s) 27, 36, 1346, 1384
BccI CCATC 3 cut(s) 552, 898, 1468
BcgI CGANNNNNNTGC 4 cut(s) 110, 144, 719, 753
BciT130I CCWGG 1 cut(s) 153
BciVI GTATCC 1 cut(s) 488
BcnI CCSGG 1 cut(s) 685
BcoDI GTCTC 2 cut(s) 120, 852
BcuI ACTAGT 1 cut(s) 23
BfaI CTAG 3 cut(s) 24, 1034, 1169
BfmI CTRYAG 2 cut(s) 676, 975
BfoI RGCGCY 1 cut(s) 1045
BfuI GTATCC 1 cut(s) 488
BisI GCNGC 8 cut(s) 16, 50, 72, 75, 1198, 1335, 1395, 1398
BlsI GCNGC 8 cut(s) 17, 51, 73, 76, 1199, 1336, 1396, 1399
BmcAI AGTACT 1 cut(s) 431
Bme1390I CCNGG 2 cut(s) 153, 685
Bme18I GGWCC 2 cut(s) 1081, 1457
BmgBI CACGTC 1 cut(s) 982
BmgT120I GGNCC 3 cut(s) 791, 1081, 1457
BmiI GGNNCC 3 cut(s) 358, 550, 1083
BmrFI CCNGG 2 cut(s) 153, 685
BmrI ACTGGG 1 cut(s) 532
BmsI GCATC 3 cut(s) 181, 372, 1564
BmuI ACTGGG 1 cut(s) 532
BpiI GAAGAC 1 cut(s) 887
BpmI CTGGAG 1 cut(s) 469
Bpu10I CCTNAGC 1 cut(s) 1493
BpuMI CCSGG 1 cut(s) 685
BsaJI CCNNGG 1 cut(s) 152
BsaWI WCCGGW 2 cut(s) 1312, 1510
Bsc4I CCNNNNNNNGG 4 cut(s) 315, 513, 558, 1196
Bse118I RCCGGY 1 cut(s) 1510
Bse1I ACTGG 2 cut(s) 527, 1148
BseAI TCCGGA 1 cut(s) 1312
BseBI CCWGG 1 cut(s) 153
BseDI CCNNGG 1 cut(s) 152
BseGI GGATG 3 cut(s) 93, 309, 1566
BseLI CCNNNNNNNGG 4 cut(s) 315, 513, 558, 1196
BseMII CTCAG 3 cut(s) 278, 1077, 1217
BseNI ACTGG 2 cut(s) 527, 1148
BseSI GKGCMC 1 cut(s) 551
BseXI GCAGC 4 cut(s) 27, 36, 1346, 1384
BseYI CCCAGC 2 cut(s) 788, 1400
Bsh1236I CGCG 1 cut(s) 120
Bsh1285I CGRYCG 1 cut(s) 104
BshFI GGCC 1 cut(s) 793
BshNI GGYRCC 1 cut(s) 548
BshTI ACCGGT 1 cut(s) 1510
BsiEI CGRYCG 1 cut(s) 104
BsiHKAI GWGCWC 1 cut(s) 522
BsiSI CCGG 3 cut(s) 684, 1313, 1511
BslFI GGGAC 1 cut(s) 1067
BslI CCNNNNNNNGG 4 cut(s) 315, 513, 558, 1196
BsmAI GTCTC 2 cut(s) 120, 852
BsmFI GGGAC 1 cut(s) 1067
BsnI GGCC 1 cut(s) 793
Bsp1286I GDGCHC 3 cut(s) 522, 551, 625
Bsp13I TCCGGA 1 cut(s) 1312
Bsp1407I TGTACA 1 cut(s) 1182
Bsp143I GATC 4 cut(s) 101, 121, 1065, 1452
Bsp68I TCGCGA 1 cut(s) 120
BspACI CCGC 6 cut(s) 72, 75, 274, 1197, 1394, 1591
BspANI GGCC 1 cut(s) 793
BspCNI CTCAG 3 cut(s) 277, 1078, 1216
BspEI TCCGGA 1 cut(s) 1312
BspFNI CGCG 1 cut(s) 120
BspLI GGNNCC 3 cut(s) 358, 550, 1083
BspQI GCTCTTC 1 cut(s) 630
BspT107I GGYRCC 1 cut(s) 548
BsrFI RCCGGY 1 cut(s) 1510
BsrGI TGTACA 1 cut(s) 1182
BsrI ACTGG 2 cut(s) 527, 1148
BssAI RCCGGY 1 cut(s) 1510
BssECI CCNNGG 1 cut(s) 152
BssMI GATC 4 cut(s) 101, 121, 1065, 1452
Bst2UI CCWGG 1 cut(s) 153
Bst4CI ACNGT 4 cut(s) 114, 138, 673, 875
Bst6I CTCTTC 1 cut(s) 630
BstAPI GCANNNNNTGC 2 cut(s) 1340, 1358
BstAUI TGTACA 1 cut(s) 1182
BstC8I GCNNGC 5 cut(s) 276, 361, 1149, 1522, 1526
BstDEI CTNAG 5 cut(s) 264, 800, 1086, 1203, 1493
BstF5I GGATG 3 cut(s) 93, 309, 1566
BstFNI CGCG 1 cut(s) 120
BstH2I RGCGCY 1 cut(s) 1045
BstHHI GCGC 2 cut(s) 591, 1044
BstKTI GATC 4 cut(s) 104, 124, 1068, 1455
BstMAI GTCTC 2 cut(s) 120, 852
BstMBI GATC 4 cut(s) 101, 121, 1065, 1452
BstMCI CGRYCG 1 cut(s) 104
BstMWI GCNNNNNNNGC 6 cut(s) 83, 597, 1340, 1349, 1358, 1403
BstNI CCWGG 1 cut(s) 153
BstNSI RCATGY 3 cut(s) 1151, 1299, 1528
BstSCI CCNGG 2 cut(s) 151, 683
BstSFI CTRYAG 2 cut(s) 676, 975
BstSLI GKGCMC 1 cut(s) 551
BstUI CGCG 1 cut(s) 120
BstV1I GCAGC 4 cut(s) 27, 36, 1346, 1384
BstV2I GAAGAC 1 cut(s) 887
BsuI GTATCC 1 cut(s) 488
BsuRI GGCC 1 cut(s) 793
BtrI CACGTC 1 cut(s) 982
BtsCI GGATG 3 cut(s) 93, 309, 1566
BtsIMutI CAGTG 6 cut(s) 396, 520, 669, 955, 1061, 1396
BtuMI TCGCGA 1 cut(s) 120
Cac8I GCNNGC 5 cut(s) 276, 361, 1149, 1522, 1526
CfoI GCGC 2 cut(s) 591, 1044
Cfr10I RCCGGY 1 cut(s) 1510
Cfr13I GGNCC 3 cut(s) 791, 1081, 1457
CseI GACGC 1 cut(s) 137
Csp6I GTAC 5 cut(s) 430, 871, 1183, 1548, 1587
CspAI ACCGGT 1 cut(s) 1510
CviQI GTAC 5 cut(s) 430, 871, 1183, 1548, 1587
DdeI CTNAG 5 cut(s) 264, 800, 1086, 1203, 1493
DpnI GATC 4 cut(s) 103, 123, 1067, 1454
DpnII GATC 4 cut(s) 101, 121, 1065, 1452
Eam1104I CTCTTC 1 cut(s) 630
EarI CTCTTC 1 cut(s) 630
Eco24I GRGCYC 1 cut(s) 625
Eco47I GGWCC 2 cut(s) 1081, 1457
Eco57I CTGAAG 1 cut(s) 372
EcoO109I RGGNCCY 1 cut(s) 1081
EcoRI GAATTC 1 cut(s) 1385
EcoRII CCWGG 1 cut(s) 151
EcoT22I ATGCAT 1 cut(s) 1345
EcoT38I GRGCYC 1 cut(s) 625
FalI AAGNNNNNCTT 2 cut(s) 725, 757
FaqI GGGAC 1 cut(s) 1067
FauI CCCGC 1 cut(s) 267
FauNDI CATATG 1 cut(s) 769
FblI GTMKAC 1 cut(s) 978
Fnu4HI GCNGC 8 cut(s) 16, 50, 72, 75, 1198, 1335, 1395, 1398
FokI GGATG 3 cut(s) 80, 316, 1553
FriOI GRGCYC 1 cut(s) 625
Fsp4HI GCNGC 8 cut(s) 16, 50, 72, 75, 1198, 1335, 1395, 1398
FspBI CTAG 3 cut(s) 24, 1034, 1169
FspI TGCGCA 1 cut(s) 590
GlaI GCGC 2 cut(s) 590, 1043
GluI GCNGC 8 cut(s) 16, 50, 72, 75, 1198, 1335, 1395, 1398
GsaI CCCAGC 2 cut(s) 792, 1404
GsuI CTGGAG 1 cut(s) 469
HaeII RGCGCY 1 cut(s) 1045
HaeIII GGCC 1 cut(s) 793
HapII CCGG 3 cut(s) 684, 1313, 1511
HgaI GACGC 1 cut(s) 137
HhaI GCGC 2 cut(s) 591, 1044
Hin6I GCGC 2 cut(s) 589, 1042
HinP1I GCGC 2 cut(s) 589, 1042
HindIII AAGCTT 6 cut(s) 598, 802, 1129, 1211, 1271, 1553
HinfI GANTC 2 cut(s) 632, 1053
HpaII CCGG 3 cut(s) 684, 1313, 1511
HphI GGTGA 1 cut(s) 223
Hpy166II GTNNAC 2 cut(s) 979, 1108
Hpy188I TCNGA 7 cut(s) 143, 190, 423, 903, 1052, 1206, 1457
Hpy188III TCNNGA 6 cut(s) 119, 448, 629, 724, 1313, 1382
Hpy8I GTNNAC 2 cut(s) 979, 1108
Hpy99I CGWCG 1 cut(s) 102
HpyAV CCTTC 3 cut(s) 97, 485, 1496
HpyCH4III ACNGT 4 cut(s) 114, 138, 673, 875
HpyCH4IV ACGT 2 cut(s) 344, 981
HpyCH4V TGCA 7 cut(s) 352, 363, 477, 669, 944, 1343, 1433
HpyF10VI GCNNNNNNNGC 6 cut(s) 83, 597, 1340, 1349, 1358, 1403
HpyF3I CTNAG 5 cut(s) 264, 800, 1086, 1203, 1493
HpySE526I ACGT 2 cut(s) 344, 981
HspAI GCGC 2 cut(s) 589, 1042
Kpn2I TCCGGA 1 cut(s) 1312
Kzo9I GATC 4 cut(s) 101, 121, 1065, 1452
LguI GCTCTTC 1 cut(s) 630
LmnI GCTCC 2 cut(s) 356, 517
Lsp1109I GCAGC 4 cut(s) 27, 36, 1346, 1384
LweI GCATC 3 cut(s) 181, 372, 1564
MaeI CTAG 3 cut(s) 24, 1034, 1169
MaeII ACGT 2 cut(s) 344, 981
MaeIII GTNAC 3 cut(s) 695, 745, 1059
MalI GATC 4 cut(s) 103, 123, 1067, 1454
MboI GATC 4 cut(s) 101, 121, 1065, 1452
MboII GAAGA 7 cut(s) 461, 617, 887, 890, 1003, 1150, 1315
MfeI CAATTG 1 cut(s) 1111
MhlI GDGCHC 3 cut(s) 522, 551, 625
MluCI AATT 6 cut(s) 217, 394, 795, 1111, 1385, 1487
Mph1103I ATGCAT 1 cut(s) 1345
MroI TCCGGA 1 cut(s) 1312
MroXI GAANNNNTTC 1 cut(s) 431
MseI TTAA 4 cut(s) 890, 968, 1007, 1532
MslI CAYNNNNRTG 3 cut(s) 169, 1285, 1569
MspA1I CMGCKG 1 cut(s) 826
MspI CCGG 3 cut(s) 684, 1313, 1511
MspR9I CCNGG 2 cut(s) 153, 685
MunI CAATTG 1 cut(s) 1111
MvaI CCWGG 1 cut(s) 153
MvnI CGCG 1 cut(s) 120
MwoI GCNNNNNNNGC 6 cut(s) 83, 597, 1340, 1349, 1358, 1403
NciI CCSGG 1 cut(s) 685
NdeI CATATG 1 cut(s) 769
NdeII GATC 4 cut(s) 101, 121, 1065, 1452
NlaIV GGNNCC 3 cut(s) 358, 550, 1083
NmuCI GTSAC 2 cut(s) 745, 1059
NruI TCGCGA 1 cut(s) 120
NsbI TGCGCA 1 cut(s) 590
NsiI ATGCAT 1 cut(s) 1345
NspI RCATGY 3 cut(s) 1151, 1299, 1528
OliI CACNNNNGTG 1 cut(s) 1569
PaeI GCATGC 2 cut(s) 1151, 1528
PciI ACATGT 1 cut(s) 1295
PciSI GCTCTTC 1 cut(s) 630
PdmI GAANNNNTTC 1 cut(s) 431
PfeI GAWTC 2 cut(s) 632, 1053
PflMI CCANNNNNTGG 1 cut(s) 315
PinAI ACCGGT 1 cut(s) 1510
PkrI GCNGC 8 cut(s) 17, 51, 73, 76, 1199, 1336, 1396, 1399
Ple19I CGATCG 1 cut(s) 104
PpuMI RGGWCCY 1 cut(s) 1081
PscI ACATGT 1 cut(s) 1295
Psp5II RGGWCCY 1 cut(s) 1081
Psp6I CCWGG 1 cut(s) 151
PspFI CCCAGC 2 cut(s) 788, 1400
PspGI CCWGG 1 cut(s) 151
PspN4I GGNNCC 3 cut(s) 358, 550, 1083
PspPI GGNCC 3 cut(s) 791, 1081, 1457
PspPPI RGGWCCY 1 cut(s) 1081
PvuI CGATCG 1 cut(s) 104
PvuII CAGCTG 1 cut(s) 826
RruI TCGCGA 1 cut(s) 120
RsaI GTAC 5 cut(s) 431, 872, 1184, 1549, 1588
RsaNI GTAC 5 cut(s) 430, 871, 1183, 1548, 1587
RseI CAYNNNNRTG 3 cut(s) 169, 1285, 1569
SapI GCTCTTC 1 cut(s) 630
SaqAI TTAA 4 cut(s) 890, 968, 1007, 1532
SatI GCNGC 8 cut(s) 16, 50, 72, 75, 1198, 1335, 1395, 1398
Sau3AI GATC 4 cut(s) 101, 121, 1065, 1452
Sau96I GGNCC 3 cut(s) 791, 1081, 1457
ScaI AGTACT 1 cut(s) 431
ScrFI CCNGG 2 cut(s) 153, 685
SduI GDGCHC 3 cut(s) 522, 551, 625
SfaNI GCATC 3 cut(s) 181, 372, 1564
SfcI CTRYAG 2 cut(s) 676, 975
SinI GGWCC 2 cut(s) 1081, 1457
SmiMI CAYNNNNRTG 3 cut(s) 169, 1285, 1569
SpeI ACTAGT 1 cut(s) 23
SphI GCATGC 2 cut(s) 1151, 1528
Sse9I AATT 6 cut(s) 217, 394, 795, 1111, 1385, 1487
SsiI CCGC 6 cut(s) 72, 75, 274, 1197, 1394, 1591
SspI AATATT 1 cut(s) 249
SspMI CTAG 3 cut(s) 24, 1034, 1169
StyD4I CCNGG 2 cut(s) 151, 683
TaaI ACNGT 4 cut(s) 114, 138, 673, 875
TaiI ACGT 2 cut(s) 347, 984
TaqI TCGA 3 cut(s) 100, 211, 642
TasI AATT 6 cut(s) 217, 394, 795, 1111, 1385, 1487
TatI WGTACW 2 cut(s) 429, 1182
TauI GCSGC 4 cut(s) 74, 77, 1200, 1397
TfiI GAWTC 2 cut(s) 632, 1053
Tru1I TTAA 4 cut(s) 890, 968, 1007, 1532
Tru9I TTAA 4 cut(s) 890, 968, 1007, 1532
TscAI CASTG 6 cut(s) 396, 527, 676, 955, 1068, 1396
TseFI GTSAC 2 cut(s) 745, 1059
TseI GCWGC 4 cut(s) 15, 49, 1334, 1397
Tsp45I GTSAC 2 cut(s) 745, 1059
TspDTI ATGAA 5 cut(s) 79, 275, 504, 1003, 1269
TspGWI ACGGA 2 cut(s) 86, 1559
TspRI CASTG 6 cut(s) 396, 527, 676, 955, 1068, 1396
Van91I CCANNNNNTGG 1 cut(s) 315
VpaK11BI GGWCC 2 cut(s) 1081, 1457
XapI RAATTY 3 cut(s) 217, 394, 1385
XceI RCATGY 3 cut(s) 1151, 1299, 1528
XmiI GTMKAC 1 cut(s) 978
XmnI GAANNNNTTC 1 cut(s) 431
XspI CTAG 3 cut(s) 24, 1034, 1169
ZrmI AGTACT 1 cut(s) 431
Zsp2I ATGCAT 1 cut(s) 1345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.