MD15G1441300.v1.1

Belongs to the membrane-bound acyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0004143
Physical Location & Seq
Reverse (-)
54100021 .. 54109605
9585 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1441300.v1.1.491

Sequence Viewer

Length: 1566 bp
ATGAAGTTGTTGAAAGGAGAGGTGGGATTTCTCATTCTCTACGCAGCTGCTTTCTATGCCTTCATCATCCATCGATCCCTCCAACTCTCTCGCGATCATCATGCTAAGCTCTTCGGTTTGCGTCCTGGATGCCTCATTCCTCATCGCCTCAATGATGTTTCTGATGCTCAATGGAGAAACTTTCGAGGAAATTTTCCTGTTCTTACTGGTGTCTTCACAATGTTCACGTTGATTGCTAATTTGCTGAGGACAAGCTTTCATTTAAAAGCGAGAGGGATGTCCATTATTTGGATTTTGATTTCTTTCACATACCTAGCATATCTGCATGGAGCCTGCATTATATTTATCCTCGCAATCGCTTCAATGAATTTTCTTTTGGTAAAGATATTTGCACGAACAAAGTATTTCCCTTTTGTATTTTGGATTTTCAACATATTCTTTCTTCTGTGCAATCGTGTTTATGAAGGATATTCATTCTCCATAATTGGGCAACGCTGGGCATATTTGGACAACTTTCGGGGCACCTTTAGATGGCACATTTGCTTTAACTTTGTTGTTTTGCGGATGATAAGCTTTGGCTATGATTACCATTGGGCACATCGAGAATCTCGTTTTGACCATAAGAAACACATTCAGCGTTGCAATATTTGTAAATCAGGGAAAACTTGTTACCATGTTTTACAGACAGGTGTCCAGAATGACATGTATGCCTTTACGACTTACCTTGCGTATTTGGTGTATGCACCTCTTTATCTTACTGGGCCAATTGTAAGCTTCAATGCATTTGCCTCACAGTTGGATGTTCCTCAAAATAACTTTTCAGCTAAGGACATTGCTTGGTATGGTTTGCGCTGGGTGTTCAGTCTTCTTCTGATGGAACTAATGACACATCTTTTCTATTACAATGCGTTTGCAATCAGTGGCATGTGGAAAGAGTTATACCCTATGGATGTGTTTATTATTGGATATGGGGTTTTAATCTTCATGTGGCTAAAGTTTTTTCTGATATGGCGCTATTTCCGGTTCTGGTCACTGATATGTGGCATTGAGGTCCCTGAGAATATGCCGAGGTGTATAAATAATTGCTACAACTTGGAAACTTTTTGGAAGAACTGGCATGCTTCCTACAACAAATGGCTTGTGAGGTATTTGTACATTCCTCTTGGGGGCACTCGGAGGAAGCTTCTCAATGTATGGGTTGTATTCACATTTGTTGCCATTTGGCATGATCTAGAATGGAAGCTTCTTTCGTGGGCATGGCTGACATGCTTATTCTTTATTCCGGAAATGATATTGAAATCAGCAACCAATGCATTTCAGGTTAAGAGTACTCATGGGGAATTTGTTGTTCGTGAACTTAGTGCGGTTGCTGGTGCAATTACTATCACTTGTCTCATGGTAGCAAACCTTGTTGGTTTTGTTATTGGACCATCTGGGATTAGTTGGTTGATTTCTCAATTCCTTAAACGAGAAGGACTTCCGGTTTTGGCAGGCATGCTTTTGACATTTTATGTAGGGACAAAGCTTATGTTCCACATCCGCGATGCAAAGCAAAGTAGCAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MBOAT

Protein Analysis

522

Amino Acids

61.18

Weight (kDa)

9.58

Isoelectric Point (pI)

36.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MBOAT PF03062 185 - 443 1.8e-38 MBOAT, membrane-bound O-acyltransferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 521
AccB7I CCANNNNNTGG 1 cut(s) 288
AccII CGCG 2 cut(s) 93, 1542
AccIII TCCGGA 1 cut(s) 1282
AciI CCGC 3 cut(s) 562, 1364, 1540
AclWI GGATC 1 cut(s) 69
AcsI RAATTY 3 cut(s) 190, 367, 1340
AfaI GTAC 2 cut(s) 1154, 1330
AfiI CCNNNNNNNGG 4 cut(s) 288, 486, 531, 1166
AflIII ACRYGT 1 cut(s) 702
AgsI TTSAA 5 cut(s) 13, 363, 430, 778, 1297
AjnI CCWGG 1 cut(s) 124
AjuI GAANNNNNNNTTGG 2 cut(s) 359, 391
Alw26I GTCTC 1 cut(s) 1397
AlwI GGATC 1 cut(s) 69
Aor13HI TCCGGA 1 cut(s) 1282
AoxI GGCC 1 cut(s) 761
ApeKI GCWGC 3 cut(s) 44, 47, 1560
ApoI RAATTY 3 cut(s) 190, 367, 1340
Asp700I GAANNNNTTC 1 cut(s) 1476
AspLEI GCGC 2 cut(s) 852, 1014
AspS9I GGNCC 3 cut(s) 761, 1051, 1427
AvaII GGWCC 2 cut(s) 1051, 1427
BaeGI GKGCMC 3 cut(s) 524, 598, 1172
BanI GGYRCC 1 cut(s) 521
BbsI GAAGAC 2 cut(s) 205, 857
BbvCI CCTCAGC 1 cut(s) 245
BbvI GCAGC 2 cut(s) 34, 56
BccI CCATC 4 cut(s) 78, 525, 868, 1438
BcgI CGANNNNNNTGC 2 cut(s) 83, 117
BciT130I CCWGG 1 cut(s) 126
BcoDI GTCTC 1 cut(s) 1397
BfaI CTAG 2 cut(s) 314, 1232
BfoI RGCGCY 1 cut(s) 1015
BisI GCNGC 3 cut(s) 45, 48, 1561
BlpI GCTNAGC 1 cut(s) 105
BlsI GCNGC 3 cut(s) 46, 49, 1562
BmcAI AGTACT 1 cut(s) 1330
Bme1390I CCNGG 1 cut(s) 126
Bme18I GGWCC 2 cut(s) 1051, 1427
BmgT120I GGNCC 3 cut(s) 761, 1051, 1427
BmiI GGNNCC 3 cut(s) 331, 523, 1053
BmrFI CCNGG 1 cut(s) 126
BmrI ACTGGG 1 cut(s) 768
BmsI GCATC 3 cut(s) 119, 154, 1534
BmuI ACTGGG 1 cut(s) 768
BoxI GACNNNNGTC 1 cut(s) 689
BpiI GAAGAC 2 cut(s) 205, 857
Bpu10I CCTNAGC 2 cut(s) 245, 825
Bpu1102I GCTNAGC 1 cut(s) 105
Bsa29I ATCGAT 1 cut(s) 73
BsaJI CCNNGG 1 cut(s) 1067
BsaWI WCCGGW 3 cut(s) 1020, 1282, 1480
Bsc4I CCNNNNNNNGG 4 cut(s) 288, 486, 531, 1166
Bse1I ACTGG 3 cut(s) 211, 763, 1118
Bse3DI GCAATG 1 cut(s) 831
BseAI TCCGGA 1 cut(s) 1282
BseBI CCWGG 1 cut(s) 126
BseCI ATCGAT 1 cut(s) 73
BseDI CCNNGG 1 cut(s) 1067
BseGI GGATG 7 cut(s) 66, 134, 282, 570, 805, 955, 1536
BseLI CCNNNNNNNGG 4 cut(s) 288, 486, 531, 1166
BseMI GCAATG 1 cut(s) 831
BseMII CTCAG 2 cut(s) 236, 1047
BseNI ACTGG 3 cut(s) 211, 763, 1118
BseSI GKGCMC 3 cut(s) 524, 598, 1172
BseXI GCAGC 2 cut(s) 34, 56
BseYI CCCAGC 2 cut(s) 495, 852
Bsh1236I CGCG 2 cut(s) 93, 1542
BshFI GGCC 1 cut(s) 763
BshNI GGYRCC 1 cut(s) 521
BshVI ATCGAT 1 cut(s) 73
BsiSI CCGG 3 cut(s) 1021, 1283, 1481
BslFI GGGAC 2 cut(s) 1037, 1531
BslI CCNNNNNNNGG 4 cut(s) 288, 486, 531, 1166
BsmAI GTCTC 1 cut(s) 1397
BsmFI GGGAC 2 cut(s) 1037, 1531
BsnI GGCC 1 cut(s) 763
Bsp1286I GDGCHC 3 cut(s) 524, 598, 1172
Bsp13I TCCGGA 1 cut(s) 1282
Bsp1407I TGTACA 1 cut(s) 1152
Bsp143I GATC 3 cut(s) 74, 94, 1228
Bsp1720I GCTNAGC 1 cut(s) 105
Bsp68I TCGCGA 1 cut(s) 93
BspACI CCGC 3 cut(s) 562, 1364, 1540
BspANI GGCC 1 cut(s) 763
BspCNI CTCAG 2 cut(s) 237, 1048
BspDI ATCGAT 1 cut(s) 73
BspEI TCCGGA 1 cut(s) 1282
BspFNI CGCG 2 cut(s) 93, 1542
BspLI GGNNCC 3 cut(s) 331, 523, 1053
BspPI GGATC 1 cut(s) 69
BspQI GCTCTTC 1 cut(s) 116
BspT107I GGYRCC 1 cut(s) 521
BsrDI GCAATG 1 cut(s) 831
BsrGI TGTACA 1 cut(s) 1152
BsrI ACTGG 3 cut(s) 211, 763, 1118
BssECI CCNNGG 1 cut(s) 1067
BssMI GATC 3 cut(s) 74, 94, 1228
Bst2UI CCWGG 1 cut(s) 126
Bst4CI ACNGT 1 cut(s) 795
Bst6I CTCTTC 1 cut(s) 116
BstAPI GCANNNNNTGC 1 cut(s) 1310
BstAUI TGTACA 1 cut(s) 1152
BstC8I GCNNGC 4 cut(s) 334, 1119, 1492, 1496
BstDEI CTNAG 5 cut(s) 105, 245, 825, 1056, 1358
BstF5I GGATG 7 cut(s) 66, 134, 282, 570, 805, 955, 1536
BstFNI CGCG 2 cut(s) 93, 1542
BstH2I RGCGCY 1 cut(s) 1015
BstHHI GCGC 2 cut(s) 852, 1014
BstKTI GATC 3 cut(s) 77, 97, 1231
BstMAI GTCTC 1 cut(s) 1397
BstMBI GATC 3 cut(s) 74, 94, 1228
BstMWI GCNNNNNNNGC 2 cut(s) 56, 1310
BstNI CCWGG 1 cut(s) 126
BstNSI RCATGY 5 cut(s) 706, 928, 1121, 1269, 1498
BstPAI GACNNNNGTC 1 cut(s) 689
BstSCI CCNGG 1 cut(s) 124
BstSLI GKGCMC 3 cut(s) 524, 598, 1172
BstUI CGCG 2 cut(s) 93, 1542
BstV1I GCAGC 2 cut(s) 34, 56
BstV2I GAAGAC 2 cut(s) 205, 857
Bsu15I ATCGAT 1 cut(s) 73
BsuRI GGCC 1 cut(s) 763
BsuTUI ATCGAT 1 cut(s) 73
BtgZI GCGATG 2 cut(s) 128, 1557
BtsCI GGATG 7 cut(s) 66, 134, 282, 570, 805, 955, 1536
BtsIMutI CAGTG 2 cut(s) 925, 1031
BtuMI TCGCGA 1 cut(s) 93
Cac8I GCNNGC 4 cut(s) 334, 1119, 1492, 1496
CfoI GCGC 2 cut(s) 852, 1014
Cfr13I GGNCC 3 cut(s) 761, 1051, 1427
ClaI ATCGAT 1 cut(s) 73
CseI GACGC 1 cut(s) 110
Csp6I GTAC 2 cut(s) 1153, 1329
CviQI GTAC 2 cut(s) 1153, 1329
DdeI CTNAG 5 cut(s) 105, 245, 825, 1056, 1358
DpnI GATC 3 cut(s) 76, 96, 1230
DpnII GATC 3 cut(s) 74, 94, 1228
DraI TTTAAA 1 cut(s) 264
Eam1104I CTCTTC 1 cut(s) 116
EarI CTCTTC 1 cut(s) 116
Eco47I GGWCC 2 cut(s) 1051, 1427
EcoO109I RGGNCCY 1 cut(s) 1051
EcoRII CCWGG 1 cut(s) 124
EcoT22I ATGCAT 2 cut(s) 784, 1315
FaqI GGGAC 2 cut(s) 1037, 1531
Fnu4HI GCNGC 3 cut(s) 45, 48, 1561
FokI GGATG 7 cut(s) 53, 141, 289, 577, 812, 962, 1523
Fsp4HI GCNGC 3 cut(s) 45, 48, 1561
FspBI CTAG 2 cut(s) 314, 1232
GlaI GCGC 2 cut(s) 851, 1013
GluI GCNGC 3 cut(s) 45, 48, 1561
GsaI CCCAGC 2 cut(s) 499, 856
HaeII RGCGCY 1 cut(s) 1015
HaeIII GGCC 1 cut(s) 763
HapII CCGG 3 cut(s) 1021, 1283, 1481
HgaI GACGC 1 cut(s) 110
HhaI GCGC 2 cut(s) 852, 1014
Hin6I GCGC 2 cut(s) 850, 1012
HinP1I GCGC 2 cut(s) 850, 1012
HindIII AAGCTT 6 cut(s) 253, 571, 772, 1181, 1241, 1523
HinfI GANTC 1 cut(s) 605
HpaII CCGG 3 cut(s) 1021, 1283, 1481
Hpy166II GTNNAC 2 cut(s) 225, 1355
Hpy188I TCNGA 4 cut(s) 163, 873, 1005, 1176
Hpy188III TCNNGA 6 cut(s) 92, 602, 694, 1232, 1283, 1352
Hpy8I GTNNAC 2 cut(s) 225, 1355
HpyAV CCTTC 3 cut(s) 70, 458, 1466
HpyCH4III ACNGT 1 cut(s) 795
HpyCH4IV ACGT 1 cut(s) 227
HpyF10VI GCNNNNNNNGC 2 cut(s) 56, 1310
HpyF3I CTNAG 5 cut(s) 105, 245, 825, 1056, 1358
HpySE526I ACGT 1 cut(s) 227
HspAI GCGC 2 cut(s) 850, 1012
Kpn2I TCCGGA 1 cut(s) 1282
Kzo9I GATC 3 cut(s) 74, 94, 1228
LguI GCTCTTC 1 cut(s) 116
LmnI GCTCC 1 cut(s) 329
Lsp1109I GCAGC 2 cut(s) 34, 56
LweI GCATC 3 cut(s) 119, 154, 1534
MaeI CTAG 2 cut(s) 314, 1232
MaeII ACGT 1 cut(s) 227
MaeIII GTNAC 2 cut(s) 668, 1029
MalI GATC 3 cut(s) 76, 96, 1230
MboI GATC 3 cut(s) 74, 94, 1228
MboII GAAGA 7 cut(s) 103, 205, 434, 857, 860, 973, 1120
MfeI CAATTG 1 cut(s) 765
MhlI GDGCHC 3 cut(s) 524, 598, 1172
MluCI AATT 9 cut(s) 190, 238, 367, 483, 765, 1081, 1340, 1377, 1457
MmeI TCCRAC 2 cut(s) 106, 777
Mph1103I ATGCAT 2 cut(s) 784, 1315
MroI TCCGGA 1 cut(s) 1282
MroXI GAANNNNTTC 1 cut(s) 1476
MseI TTAA 5 cut(s) 263, 546, 977, 1323, 1464
MslI CAYNNNNRTG 1 cut(s) 1036
MspA1I CMGCKG 1 cut(s) 47
MspI CCGG 3 cut(s) 1021, 1283, 1481
MspR9I CCNGG 1 cut(s) 126
MunI CAATTG 1 cut(s) 765
MvaI CCWGG 1 cut(s) 126
MvnI CGCG 2 cut(s) 93, 1542
MwoI GCNNNNNNNGC 2 cut(s) 56, 1310
NdeII GATC 3 cut(s) 74, 94, 1228
NlaIV GGNNCC 3 cut(s) 331, 523, 1053
NmeAIII GCCGAG 1 cut(s) 1092
NmuCI GTSAC 1 cut(s) 1029
NruI TCGCGA 1 cut(s) 93
NsiI ATGCAT 2 cut(s) 784, 1315
NspI RCATGY 5 cut(s) 706, 928, 1121, 1269, 1498
PaeI GCATGC 2 cut(s) 1121, 1498
PciI ACATGT 1 cut(s) 702
PciSI GCTCTTC 1 cut(s) 116
PcsI WCGNNNNNNNCGW 1 cut(s) 607
PdmI GAANNNNTTC 1 cut(s) 1476
PfeI GAWTC 1 cut(s) 605
PflMI CCANNNNNTGG 1 cut(s) 288
PfoI TCCNGGA 1 cut(s) 124
PkrI GCNGC 3 cut(s) 46, 49, 1562
PpuMI RGGWCCY 1 cut(s) 1051
PscI ACATGT 1 cut(s) 702
PshAI GACNNNNGTC 1 cut(s) 689
Psp5II RGGWCCY 1 cut(s) 1051
Psp6I CCWGG 1 cut(s) 124
PspFI CCCAGC 2 cut(s) 495, 852
PspGI CCWGG 1 cut(s) 124
PspN4I GGNNCC 3 cut(s) 331, 523, 1053
PspPI GGNCC 3 cut(s) 761, 1051, 1427
PspPPI RGGWCCY 1 cut(s) 1051
PvuII CAGCTG 1 cut(s) 47
RruI TCGCGA 1 cut(s) 93
RsaI GTAC 2 cut(s) 1154, 1330
RsaNI GTAC 2 cut(s) 1153, 1329
RseI CAYNNNNRTG 1 cut(s) 1036
SapI GCTCTTC 1 cut(s) 116
SaqAI TTAA 5 cut(s) 263, 546, 977, 1323, 1464
SatI GCNGC 3 cut(s) 45, 48, 1561
Sau3AI GATC 3 cut(s) 74, 94, 1228
Sau96I GGNCC 3 cut(s) 761, 1051, 1427
ScaI AGTACT 1 cut(s) 1330
ScrFI CCNGG 1 cut(s) 126
SduI GDGCHC 3 cut(s) 524, 598, 1172
SfaNI GCATC 3 cut(s) 119, 154, 1534
SinI GGWCC 2 cut(s) 1051, 1427
SmiMI CAYNNNNRTG 1 cut(s) 1036
SphI GCATGC 2 cut(s) 1121, 1498
Sse9I AATT 9 cut(s) 190, 238, 367, 483, 765, 1081, 1340, 1377, 1457
SsiI CCGC 3 cut(s) 562, 1364, 1540
SspI AATATT 1 cut(s) 646
SspMI CTAG 2 cut(s) 314, 1232
StyD4I CCNGG 1 cut(s) 124
TaaI ACNGT 1 cut(s) 795
TaiI ACGT 1 cut(s) 230
TaqI TCGA 3 cut(s) 73, 184, 601
TasI AATT 9 cut(s) 190, 238, 367, 483, 765, 1081, 1340, 1377, 1457
TatI WGTACW 2 cut(s) 1152, 1328
TfiI GAWTC 1 cut(s) 605
Tru1I TTAA 5 cut(s) 263, 546, 977, 1323, 1464
Tru9I TTAA 5 cut(s) 263, 546, 977, 1323, 1464
TscAI CASTG 2 cut(s) 925, 1038
TseFI GTSAC 1 cut(s) 1029
TseI GCWGC 3 cut(s) 44, 47, 1560
Tsp45I GTSAC 1 cut(s) 1029
TspDTI ATGAA 7 cut(s) 17, 52, 248, 380, 462, 477, 973
TspRI CASTG 2 cut(s) 925, 1038
Van91I CCANNNNNTGG 1 cut(s) 288
VpaK11BI GGWCC 2 cut(s) 1051, 1427
XapI RAATTY 3 cut(s) 190, 367, 1340
XbaI TCTAGA 1 cut(s) 1231
XceI RCATGY 5 cut(s) 706, 928, 1121, 1269, 1498
XmnI GAANNNNTTC 1 cut(s) 1476
XspI CTAG 2 cut(s) 314, 1232
ZrmI AGTACT 1 cut(s) 1330
Zsp2I ATGCAT 2 cut(s) 784, 1315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.