MD08G1246100.v1.1

Belongs to the membrane-bound acyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0004143
Physical Location & Seq
Reverse (-)
31045633 .. 31056265
10633 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1246100.v1.1.491

Sequence Viewer

Length: 1569 bp
ATGAAGTTGTTGAAAGGAGAGGTGGGATTTCTGATTCTCTACGCCGCTGCTTTCTACGCCTTCATCATCCATCGATCCCTCCAAATCTCTCGCGATCATCACGCTAAGCTTTTCGGTTTGCGTCCTGGATGGCTCATTCCTCCTCGCCTCAATGATGTTTCTGATGCTCAATGGAGAAACTTTCGAGGAAATTTACCAATTCTTACCGTTGTTTTCACAATGTTCACGTTGATCGCTAATTTTTTGAGGACAAGCTTTCATTTAAAAGCGAGAGGGATGTCCATTATTTGGATTTTGATTTCTTTCACATACCTAGCATATCTGCATGGAGCCTGCATTCTATTTGTCCTCTCAATCGCTTCAATGAATTTTCTCTTGGTAAAGATATTTGCACGAACAAAATATTTCCCTTTTGTATTATGGATTTTCAACATATTCTTTCTTCTGTGCAATCGTGTTTATGAAGGATATTCATTCTCCATAATTGGGCAACGCTGGGCATATTTGGACAACTTTCGGGGCACCTTTAGATGGCACATTTGCTTTAACTTTGTTGTTTTGCGGATGATAAGCTTTGGTTATGATTACCATTGGGCACATCGAGATTCTCGTTTTGACCATAAGAAACACATTCAGCGCTGCAATATTTGTAAATCAGGGAAAACTTGTTATCATGTTTTACAGGAGAGAGGTGTCCAGCATGACAAGTATGCCTTTACAACATACCTTGCTTATTTGGTGTATGCACCTCTTTATCTTACTGGGCCAATTGTTAGCTTCAATGCATTTGCCTCACAGTTGGATGTTCCTCAAAATAACTTTTCAGCTAAAGACATTGCTTTGTATGGTTTGCGCTGGGTGTTCAGTCTTCTTCTGATGGAACTAATGACACATCTTTTCTATTACAATGCGTTTGCAATCAGTGGCATGTGGAAAGAGTTACATCCTATGGATGTGTTTATTATTGGATATGGGGTCTTAATGTTCATGTGGCTGAAGTTTTTTCTGATATGGCGCTATTTCCGGTTCTGGTCACTGATATGTGGCATTGAGGTCCCGGAGAATATGCCGAGGTGTATAAATAATTGCTACAACTTGGAAACTTTTTGGAAAAACTGGCATGCTTCCTACAACAAATGGCTTGTGAGGTATTTGTACATTCCTCTTGGGGGCACTCAGAGAAAGCTTCTCAATGTATGGGTTGTATTCACATTTGTTGCCATTTGGCATGATCTAGAATGGAAGCTTCTTTCATGGGCATGGCTGACATGCTTATTCTTTATTCCGGAAATGATACTGAAATCAGCAACCAATGCATTTCAGTTTAAGAGTACACATGGTGAGTTTGTTGTTCGTGAACTTAGCGCGGTTGCTGGTGCAATCACGATCACTTGCCTCATGGTAGCAAACCTTGTTGGTTTTGTTATTGGACCATCTGGGATTAGTTGGTTGATTTCTCAATTCCTTAAACGAGAAGGACTCCCCGTTTTGGCTGGCATGCTTTTGACATTTTATGTAGGGACAAAGCTTATGTTCCACATCCGTGATGCAAAGCAAAGTAGCAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MBOAT

Protein Analysis

523

Amino Acids

61.38

Weight (kDa)

9.61

Isoelectric Point (pI)

35.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MBOAT PF03062 183 - 444 1e-40 MBOAT, membrane-bound O-acyltransferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 521
AccB7I CCANNNNNTGG 1 cut(s) 288
AccII CGCG 2 cut(s) 93, 1367
AccIII TCCGGA 1 cut(s) 1285
AciI CCGC 3 cut(s) 45, 562, 1367
AclWI GGATC 1 cut(s) 69
AcsI RAATTY 2 cut(s) 190, 367
AcuI CTGAAG 1 cut(s) 1016
AdeI CACNNNGTG 1 cut(s) 1340
AfaI GTAC 2 cut(s) 1157, 1333
AfeI AGCGCT 1 cut(s) 638
AfiI CCNNNNNNNGG 5 cut(s) 288, 486, 531, 1169, 1489
AgsI TTSAA 4 cut(s) 13, 363, 430, 781
AjnI CCWGG 1 cut(s) 124
AjuI GAANNNNNNNTTGG 2 cut(s) 359, 391
AleI CACNNNNGTG 1 cut(s) 1542
AluBI AGCT 9 cut(s) 109, 255, 573, 777, 827, 1186, 1246, 1528, 1566
AluI AGCT 9 cut(s) 109, 255, 573, 777, 827, 1186, 1246, 1528, 1566
AlwI GGATC 1 cut(s) 69
Aor13HI TCCGGA 1 cut(s) 1285
Aor51HI AGCGCT 1 cut(s) 638
AoxI GGCC 1 cut(s) 764
ApeKI GCWGC 3 cut(s) 47, 639, 1563
ApoI RAATTY 2 cut(s) 190, 367
AspLEI GCGC 4 cut(s) 639, 855, 1017, 1367
AspS9I GGNCC 3 cut(s) 764, 1054, 1430
AsuC2I CCSGG 1 cut(s) 1058
AsuHPI GGTGA 1 cut(s) 1352
AvaII GGWCC 2 cut(s) 1054, 1430
BaeGI GKGCMC 3 cut(s) 524, 598, 1175
BanI GGYRCC 1 cut(s) 521
BbsI GAAGAC 1 cut(s) 860
BbvI GCAGC 2 cut(s) 34, 626
BccI CCATC 5 cut(s) 78, 123, 525, 871, 1441
BciT130I CCWGG 1 cut(s) 126
BcnI CCSGG 1 cut(s) 1058
BfaI CTAG 2 cut(s) 314, 1235
BfoI RGCGCY 2 cut(s) 640, 1018
BisI GCNGC 4 cut(s) 45, 48, 640, 1564
BlpI GCTNAGC 1 cut(s) 105
BlsI GCNGC 4 cut(s) 46, 49, 641, 1565
Bme1390I CCNGG 2 cut(s) 126, 1058
Bme18I GGWCC 2 cut(s) 1054, 1430
BmgT120I GGNCC 3 cut(s) 764, 1054, 1430
BmiI GGNNCC 3 cut(s) 331, 523, 1056
BmrFI CCNGG 2 cut(s) 126, 1058
BmrI ACTGGG 1 cut(s) 771
BmsI GCATC 2 cut(s) 154, 1537
BmuI ACTGGG 1 cut(s) 771
BpiI GAAGAC 1 cut(s) 860
BplI GAGNNNNNCTC 2 cut(s) 1464, 1496
Bpu1102I GCTNAGC 1 cut(s) 105
BpuMI CCSGG 1 cut(s) 1058
Bsa29I ATCGAT 1 cut(s) 73
BsaJI CCNNGG 1 cut(s) 1070
BsaWI WCCGGW 2 cut(s) 1023, 1285
Bsc4I CCNNNNNNNGG 5 cut(s) 288, 486, 531, 1169, 1489
Bse1I ACTGG 2 cut(s) 766, 1121
Bse3DI GCAATG 1 cut(s) 834
BseAI TCCGGA 1 cut(s) 1285
BseBI CCWGG 1 cut(s) 126
BseCI ATCGAT 1 cut(s) 73
BseDI CCNNGG 1 cut(s) 1070
BseGI GGATG 8 cut(s) 66, 134, 282, 570, 808, 943, 958, 1539
BseLI CCNNNNNNNGG 5 cut(s) 288, 486, 531, 1169, 1489
BseMI GCAATG 1 cut(s) 834
BseMII CTCAG 1 cut(s) 1190
BseNI ACTGG 2 cut(s) 766, 1121
BseRI GAGGAG 1 cut(s) 132
BseSI GKGCMC 3 cut(s) 524, 598, 1175
BseXI GCAGC 2 cut(s) 34, 626
BseYI CCCAGC 2 cut(s) 495, 855
Bsh1236I CGCG 2 cut(s) 93, 1367
BshFI GGCC 1 cut(s) 766
BshNI GGYRCC 1 cut(s) 521
BshVI ATCGAT 1 cut(s) 73
BsiSI CCGG 3 cut(s) 1024, 1058, 1286
BslFI GGGAC 2 cut(s) 1040, 1534
BslI CCNNNNNNNGG 5 cut(s) 288, 486, 531, 1169, 1489
BsmFI GGGAC 2 cut(s) 1040, 1534
BsmI GAATGC 1 cut(s) 336
BsnI GGCC 1 cut(s) 766
Bsp1286I GDGCHC 3 cut(s) 524, 598, 1175
Bsp13I TCCGGA 1 cut(s) 1285
Bsp1407I TGTACA 1 cut(s) 1155
Bsp143I GATC 5 cut(s) 74, 94, 231, 1231, 1386
Bsp1720I GCTNAGC 1 cut(s) 105
Bsp68I TCGCGA 1 cut(s) 93
BspACI CCGC 3 cut(s) 45, 562, 1367
BspANI GGCC 1 cut(s) 766
BspCNI CTCAG 1 cut(s) 1189
BspDI ATCGAT 1 cut(s) 73
BspEI TCCGGA 1 cut(s) 1285
BspFNI CGCG 2 cut(s) 93, 1367
BspLI GGNNCC 3 cut(s) 331, 523, 1056
BspPI GGATC 1 cut(s) 69
BspT107I GGYRCC 1 cut(s) 521
BsrDI GCAATG 1 cut(s) 834
BsrGI TGTACA 1 cut(s) 1155
BsrI ACTGG 2 cut(s) 766, 1121
BssECI CCNNGG 1 cut(s) 1070
BssMI GATC 5 cut(s) 74, 94, 231, 1231, 1386
Bst2UI CCWGG 1 cut(s) 126
Bst4CI ACNGT 2 cut(s) 208, 798
BstAPI GCANNNNNTGC 1 cut(s) 1313
BstAUI TGTACA 1 cut(s) 1155
BstC8I GCNNGC 4 cut(s) 334, 1122, 1495, 1499
BstDEI CTNAG 3 cut(s) 105, 1176, 1361
BstF5I GGATG 8 cut(s) 66, 134, 282, 570, 808, 943, 958, 1539
BstFNI CGCG 2 cut(s) 93, 1367
BstH2I RGCGCY 2 cut(s) 640, 1018
BstHHI GCGC 4 cut(s) 639, 855, 1017, 1367
BstKTI GATC 5 cut(s) 77, 97, 234, 1234, 1389
BstMBI GATC 5 cut(s) 74, 94, 231, 1231, 1386
BstMWI GCNNNNNNNGC 2 cut(s) 56, 1313
BstNI CCWGG 1 cut(s) 126
BstNSI RCATGY 4 cut(s) 931, 1124, 1272, 1501
BstSCI CCNGG 2 cut(s) 124, 1056
BstSLI GKGCMC 3 cut(s) 524, 598, 1175
BstUI CGCG 2 cut(s) 93, 1367
BstV1I GCAGC 2 cut(s) 34, 626
BstV2I GAAGAC 1 cut(s) 860
Bsu15I ATCGAT 1 cut(s) 73
BsuRI GGCC 1 cut(s) 766
BsuTUI ATCGAT 1 cut(s) 73
BtsCI GGATG 8 cut(s) 66, 134, 282, 570, 808, 943, 958, 1539
BtsIMutI CAGTG 2 cut(s) 928, 1034
BtuMI TCGCGA 1 cut(s) 93
Cac8I GCNNGC 4 cut(s) 334, 1122, 1495, 1499
CfoI GCGC 4 cut(s) 639, 855, 1017, 1367
Cfr13I GGNCC 3 cut(s) 764, 1054, 1430
ClaI ATCGAT 1 cut(s) 73
CseI GACGC 1 cut(s) 110
Csp6I GTAC 2 cut(s) 1156, 1332
CviQI GTAC 2 cut(s) 1156, 1332
DdeI CTNAG 3 cut(s) 105, 1176, 1361
DpnI GATC 5 cut(s) 76, 96, 233, 1233, 1388
DpnII GATC 5 cut(s) 74, 94, 231, 1231, 1386
DraI TTTAAA 1 cut(s) 264
DraIII CACNNNGTG 1 cut(s) 1340
Eco47I GGWCC 2 cut(s) 1054, 1430
Eco47III AGCGCT 1 cut(s) 638
Eco57I CTGAAG 1 cut(s) 1016
EcoO109I RGGNCCY 1 cut(s) 1054
EcoRII CCWGG 1 cut(s) 124
EcoT22I ATGCAT 2 cut(s) 787, 1318
FalI AAGNNNNNCTT 2 cut(s) 698, 730
FaqI GGGAC 2 cut(s) 1040, 1534
Fnu4HI GCNGC 4 cut(s) 45, 48, 640, 1564
FokI GGATG 8 cut(s) 53, 141, 289, 577, 815, 930, 965, 1526
Fsp4HI GCNGC 4 cut(s) 45, 48, 640, 1564
FspBI CTAG 2 cut(s) 314, 1235
GlaI GCGC 4 cut(s) 638, 854, 1016, 1366
GluI GCNGC 4 cut(s) 45, 48, 640, 1564
GsaI CCCAGC 2 cut(s) 499, 859
HaeII RGCGCY 2 cut(s) 640, 1018
HaeIII GGCC 1 cut(s) 766
HapII CCGG 3 cut(s) 1024, 1058, 1286
HgaI GACGC 1 cut(s) 110
HhaI GCGC 4 cut(s) 639, 855, 1017, 1367
Hin6I GCGC 4 cut(s) 637, 853, 1015, 1365
HinP1I GCGC 4 cut(s) 637, 853, 1015, 1365
HindIII AAGCTT 6 cut(s) 107, 253, 571, 1184, 1244, 1526
HinfI GANTC 3 cut(s) 34, 605, 1479
HpaII CCGG 3 cut(s) 1024, 1058, 1286
HphI GGTGA 1 cut(s) 1352
Hpy166II GTNNAC 3 cut(s) 225, 1334, 1358
Hpy188I TCNGA 5 cut(s) 33, 163, 876, 1008, 1179
Hpy188III TCNNGA 6 cut(s) 92, 602, 1235, 1286, 1355, 1384
Hpy8I GTNNAC 3 cut(s) 225, 1334, 1358
HpyAV CCTTC 3 cut(s) 70, 458, 1469
HpyCH4III ACNGT 2 cut(s) 208, 798
HpyCH4IV ACGT 1 cut(s) 227
HpyF10VI GCNNNNNNNGC 2 cut(s) 56, 1313
HpyF3I CTNAG 3 cut(s) 105, 1176, 1361
HpySE526I ACGT 1 cut(s) 227
HspAI GCGC 4 cut(s) 637, 853, 1015, 1365
Kpn2I TCCGGA 1 cut(s) 1285
Kzo9I GATC 5 cut(s) 74, 94, 231, 1231, 1386
LmnI GCTCC 1 cut(s) 329
Lsp1109I GCAGC 2 cut(s) 34, 626
LweI GCATC 2 cut(s) 154, 1537
MaeI CTAG 2 cut(s) 314, 1235
MaeII ACGT 1 cut(s) 227
MaeIII GTNAC 2 cut(s) 939, 1032
MalI GATC 5 cut(s) 76, 96, 233, 1233, 1388
MboI GATC 5 cut(s) 74, 94, 231, 1231, 1386
MboII GAAGA 3 cut(s) 434, 860, 863
MfeI CAATTG 1 cut(s) 768
MhlI GDGCHC 3 cut(s) 524, 598, 1175
MluCI AATT 8 cut(s) 190, 198, 238, 367, 483, 768, 1084, 1460
MlyI GAGTC 1 cut(s) 1473
MmeI TCCRAC 1 cut(s) 780
Mph1103I ATGCAT 2 cut(s) 787, 1318
MroI TCCGGA 1 cut(s) 1285
MseI TTAA 5 cut(s) 263, 546, 980, 1326, 1467
MslI CAYNNNNRTG 3 cut(s) 1039, 1258, 1542
MspA1I CMGCKG 1 cut(s) 47
MspI CCGG 3 cut(s) 1024, 1058, 1286
MspR9I CCNGG 2 cut(s) 126, 1058
MunI CAATTG 1 cut(s) 768
Mva1269I GAATGC 1 cut(s) 336
MvaI CCWGG 1 cut(s) 126
MvnI CGCG 2 cut(s) 93, 1367
MwoI GCNNNNNNNGC 2 cut(s) 56, 1313
NciI CCSGG 1 cut(s) 1058
NdeII GATC 5 cut(s) 74, 94, 231, 1231, 1386
NlaIV GGNNCC 3 cut(s) 331, 523, 1056
NmeAIII GCCGAG 1 cut(s) 1095
NmuCI GTSAC 1 cut(s) 1032
NruI TCGCGA 1 cut(s) 93
NsiI ATGCAT 2 cut(s) 787, 1318
NspI RCATGY 4 cut(s) 931, 1124, 1272, 1501
OliI CACNNNNGTG 1 cut(s) 1542
PaeI GCATGC 2 cut(s) 1124, 1501
PcsI WCGNNNNNNNCGW 1 cut(s) 607
PctI GAATGC 1 cut(s) 336
PfeI GAWTC 2 cut(s) 34, 605
PflMI CCANNNNNTGG 1 cut(s) 288
PfoI TCCNGGA 2 cut(s) 124, 1056
PkrI GCNGC 4 cut(s) 46, 49, 641, 1565
PleI GAGTC 1 cut(s) 1473
PpsI GAGTC 1 cut(s) 1473
PpuMI RGGWCCY 1 cut(s) 1054
Psp5II RGGWCCY 1 cut(s) 1054
Psp6I CCWGG 1 cut(s) 124
PspFI CCCAGC 2 cut(s) 495, 855
PspGI CCWGG 1 cut(s) 124
PspN4I GGNNCC 3 cut(s) 331, 523, 1056
PspPI GGNCC 3 cut(s) 764, 1054, 1430
PspPPI RGGWCCY 1 cut(s) 1054
RruI TCGCGA 1 cut(s) 93
RsaI GTAC 2 cut(s) 1157, 1333
RsaNI GTAC 2 cut(s) 1156, 1332
RseI CAYNNNNRTG 3 cut(s) 1039, 1258, 1542
SaqAI TTAA 5 cut(s) 263, 546, 980, 1326, 1467
SatI GCNGC 4 cut(s) 45, 48, 640, 1564
Sau3AI GATC 5 cut(s) 74, 94, 231, 1231, 1386
Sau96I GGNCC 3 cut(s) 764, 1054, 1430
SchI GAGTC 1 cut(s) 1473
ScrFI CCNGG 2 cut(s) 126, 1058
SduI GDGCHC 3 cut(s) 524, 598, 1175
SfaNI GCATC 2 cut(s) 154, 1537
SinI GGWCC 2 cut(s) 1054, 1430
SmiMI CAYNNNNRTG 3 cut(s) 1039, 1258, 1542
SphI GCATGC 2 cut(s) 1124, 1501
Sse9I AATT 8 cut(s) 190, 198, 238, 367, 483, 768, 1084, 1460
SsiI CCGC 3 cut(s) 45, 562, 1367
SspI AATATT 2 cut(s) 404, 646
SspMI CTAG 2 cut(s) 314, 1235
StyD4I CCNGG 2 cut(s) 124, 1056
TaaI ACNGT 2 cut(s) 208, 798
TaiI ACGT 1 cut(s) 230
TaqI TCGA 3 cut(s) 73, 184, 601
TasI AATT 8 cut(s) 190, 198, 238, 367, 483, 768, 1084, 1460
TatI WGTACW 2 cut(s) 1155, 1331
TauI GCSGC 1 cut(s) 47
TfiI GAWTC 2 cut(s) 34, 605
Tru1I TTAA 5 cut(s) 263, 546, 980, 1326, 1467
Tru9I TTAA 5 cut(s) 263, 546, 980, 1326, 1467
TscAI CASTG 2 cut(s) 928, 1041
TseFI GTSAC 1 cut(s) 1032
TseI GCWGC 3 cut(s) 47, 639, 1563
Tsp45I GTSAC 1 cut(s) 1032
TspDTI ATGAA 8 cut(s) 17, 52, 248, 380, 462, 477, 976, 1242
TspGWI ACGGA 1 cut(s) 1532
TspRI CASTG 2 cut(s) 928, 1041
Van91I CCANNNNNTGG 1 cut(s) 288
VpaK11BI GGWCC 2 cut(s) 1054, 1430
XapI RAATTY 2 cut(s) 190, 367
XbaI TCTAGA 1 cut(s) 1234
XceI RCATGY 4 cut(s) 931, 1124, 1272, 1501
XspI CTAG 2 cut(s) 314, 1235
Zsp2I ATGCAT 2 cut(s) 787, 1318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.