FvH4_7g16810

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0016007
Physical Location & Seq
Forward (+)
14364300 .. 14365229
930 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g16810.t1

Sequence Viewer

Length: 597 bp
ATGTACATGGAGCAACCACCATTCACTGAAGATGACCTGATCACTCCCACCACCACCGCTGCCGCAGCCACCACCACCACCACCACCCAAGATAATGAAAACCAACAACGCACAGGCACCCGGCACCCGGTTTATCGTGGCGTGAGGAAGCGGCGGTGGGGGAAATGGGTGTCCGAAATCAGGGAGCCACGCAAGAAGTCACGCATTTGGCTAGGGTCCTTTCCGGTGCCCGAGATGGCAGCCAAGGCTTATGACGTGGCGGCATACTGCCTCAAAGGGCGGAAAGCGCAGCTGAACTTCCCCGACGAGGTGGAGCACTTGCCGAGGCCCTCCACCTGTACGGCTAGGGATATTCAGGCGGCAGCAGCTAAAGCAGCACAGACTATGAAGTCATCAAGTACAGCTAGCAGAAGCACTGATGATCAGGATCATCACCGTGATGTATCGGACGACGGCAGCGCCGATGACTTTTGGGGTGAGATTGAACTTCCGGAGCTGATGAACAGCGGGACATGCTATTCGAACTCATGTGGTTGGAGTACGTTTTCCGGTGAGGCAGCAGCATTAACATGGCCAGAAGGAGAGGCATGTCTATAA

Protein Analysis

199

Amino Acids

21.98

Weight (kDa)

5.43

Isoelectric Point (pI)

53.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 43 - 93 1.1e-13 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016007)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01250
fragaria_vesca FvH4_7g16810
malus_domestica MD01G1083000.v1.1 MD07G1151700.v1.1
prunus_persica Prupe.2G190200_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0360021
rosa_laevigata RLG00000027838
rosa_multiflora Rmu_ssc0000368.1_g000032
rosa_roxburghii Rroxscaffold_4G00296450
rosa_rugosa Rorug01G0274600
rosa_samantha Rh1AG288400 Rh1BG253900 Rh1CG271400 Rh1DG283000
rosa_wichuraiana Rw1G025570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 388
AccB1I GGYRCC 3 cut(s) 116, 123, 226
AccIII TCCGGA 1 cut(s) 490
AciI CCGC 8 cut(s) 57, 63, 151, 154, 260, 280, 359, 507
AclWI GGATC 1 cut(s) 435
AcoI YGGCCR 1 cut(s) 572
AcuI CTGAAG 1 cut(s) 48
AfaI GTAC 4 cut(s) 5, 340, 400, 541
AfiI CCNNNNNNNGG 3 cut(s) 127, 180, 307
AgsI TTSAA 1 cut(s) 485
AjiI CACGTC 1 cut(s) 256
AluBI AGCT 4 cut(s) 292, 368, 404, 496
AluI AGCT 4 cut(s) 292, 368, 404, 496
Alw21I GWGCWC 1 cut(s) 318
AlwI GGATC 1 cut(s) 435
Ama87I CYCGRG 1 cut(s) 230
Aor13HI TCCGGA 1 cut(s) 490
AoxI GGCC 2 cut(s) 326, 572
ArsI GACNNNNNNTTYG 2 cut(s) 502, 534
AspLEI GCGC 2 cut(s) 289, 461
AspS9I GGNCC 2 cut(s) 216, 327
AsuC2I CCSGG 2 cut(s) 121, 128
AsuHPI GGTGA 3 cut(s) 425, 488, 563
AsuII TTCGAA 1 cut(s) 521
AsuNHI GCTAGC 1 cut(s) 404
AvaI CYCGRG 1 cut(s) 230
AvaII GGWCC 1 cut(s) 216
BaeGI GKGCMC 1 cut(s) 231
BalI TGGCCA 1 cut(s) 574
BanI GGYRCC 3 cut(s) 116, 123, 226
Bbv12I GWGCWC 1 cut(s) 318
BccI CCATC 1 cut(s) 229
BceAI ACGGC 2 cut(s) 357, 469
BclI TGATCA 2 cut(s) 39, 421
BcnI CCSGG 2 cut(s) 121, 128
BfaI CTAG 3 cut(s) 212, 345, 405
BfoI RGCGCY 1 cut(s) 462
Bme1390I CCNGG 2 cut(s) 121, 128
Bme18I GGWCC 1 cut(s) 216
BmeT110I CYCGRG 1 cut(s) 230
BmgBI CACGTC 1 cut(s) 256
BmgT120I GGNCC 2 cut(s) 216, 327
BmiI GGNNCC 5 cut(s) 118, 125, 186, 217, 228
BmrFI CCNGG 2 cut(s) 121, 128
BmtI GCTAGC 1 cut(s) 408
Bpu14I TTCGAA 1 cut(s) 521
BpuMI CCSGG 2 cut(s) 121, 128
BsaBI GATNNNNATC 1 cut(s) 426
BsaJI CCNNGG 2 cut(s) 243, 323
BsaWI WCCGGW 3 cut(s) 223, 490, 548
Bsc4I CCNNNNNNNGG 3 cut(s) 127, 180, 307
Bse8I GATNNNNATC 1 cut(s) 426
BseAI TCCGGA 1 cut(s) 490
BseDI CCNNGG 2 cut(s) 243, 323
BseJI GATNNNNATC 1 cut(s) 426
BseLI CCNNNNNNNGG 3 cut(s) 127, 180, 307
BseSI GKGCMC 1 cut(s) 231
BshFI GGCC 2 cut(s) 328, 574
BshNI GGYRCC 3 cut(s) 116, 123, 226
BsiHKAI GWGCWC 1 cut(s) 318
BsiHKCI CYCGRG 1 cut(s) 230
BsiSI CCGG 5 cut(s) 121, 128, 224, 491, 549
BslFI GGGAC 1 cut(s) 523
BslI CCNNNNNNNGG 3 cut(s) 127, 180, 307
BsmFI GGGAC 1 cut(s) 523
BsnI GGCC 2 cut(s) 328, 574
BsoBI CYCGRG 1 cut(s) 230
Bsp119I TTCGAA 1 cut(s) 521
Bsp1286I GDGCHC 2 cut(s) 231, 318
Bsp13I TCCGGA 1 cut(s) 490
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 3 cut(s) 39, 421, 427
BspACI CCGC 8 cut(s) 57, 63, 151, 154, 260, 280, 359, 507
BspANI GGCC 2 cut(s) 328, 574
BspEI TCCGGA 1 cut(s) 490
BspLI GGNNCC 5 cut(s) 118, 125, 186, 217, 228
BspOI GCTAGC 1 cut(s) 408
BspPI GGATC 1 cut(s) 435
BspT104I TTCGAA 1 cut(s) 521
BspT107I GGYRCC 3 cut(s) 116, 123, 226
BsrGI TGTACA 1 cut(s) 3
BssECI CCNNGG 2 cut(s) 243, 323
BssMI GATC 3 cut(s) 39, 421, 427
BssT1I CCWWGG 1 cut(s) 243
Bst4CI ACNGT 1 cut(s) 437
BstAUI TGTACA 1 cut(s) 3
BstBI TTCGAA 1 cut(s) 521
BstC8I GCNNGC 1 cut(s) 406
BstH2I RGCGCY 1 cut(s) 462
BstHHI GCGC 2 cut(s) 289, 461
BstKTI GATC 3 cut(s) 42, 424, 430
BstMBI GATC 3 cut(s) 39, 421, 427
BstMWI GCNNNNNNNGC 7 cut(s) 65, 245, 286, 365, 371, 374, 513
BstNSI RCATGY 2 cut(s) 516, 591
BstSCI CCNGG 2 cut(s) 119, 126
BstSLI GKGCMC 1 cut(s) 231
BsuRI GGCC 2 cut(s) 328, 574
BtrI CACGTC 1 cut(s) 256
BtsIMutI CAGTG 2 cut(s) 24, 414
Cac8I GCNNGC 1 cut(s) 406
CfoI GCGC 2 cut(s) 289, 461
Cfr13I GGNCC 2 cut(s) 216, 327
Csp6I GTAC 4 cut(s) 4, 339, 399, 540
CviAII CATG 5 cut(s) 7, 513, 528, 570, 588
CviQI GTAC 4 cut(s) 4, 339, 399, 540
DpnI GATC 3 cut(s) 41, 423, 429
DpnII GATC 3 cut(s) 39, 421, 427
DrdI GACNNNNNNGTC 1 cut(s) 388
DseDI GACNNNNNNGTC 1 cut(s) 388
EaeI YGGCCR 1 cut(s) 572
EciI GGCGGA 1 cut(s) 295
Eco130I CCWWGG 1 cut(s) 243
Eco47I GGWCC 1 cut(s) 216
Eco57I CTGAAG 1 cut(s) 48
Eco88I CYCGRG 1 cut(s) 230
EcoO109I RGGNCCY 2 cut(s) 216, 327
EcoT14I CCWWGG 1 cut(s) 243
ErhI CCWWGG 1 cut(s) 243
FaeI CATG 5 cut(s) 10, 516, 531, 573, 591
FaiI YATR 9 cut(s) 8, 252, 265, 386, 514, 529, 571, 589, 595
FaqI GGGAC 1 cut(s) 523
FatI CATG 5 cut(s) 6, 512, 527, 569, 587
FauI CCCGC 1 cut(s) 500
FbaI TGATCA 2 cut(s) 39, 421
FspBI CTAG 3 cut(s) 212, 345, 405
GlaI GCGC 2 cut(s) 288, 460
HaeII RGCGCY 1 cut(s) 462
HaeIII GGCC 2 cut(s) 328, 574
HapII CCGG 5 cut(s) 121, 128, 224, 491, 549
HhaI GCGC 2 cut(s) 289, 461
Hin1II CATG 5 cut(s) 10, 516, 531, 573, 591
Hin6I GCGC 2 cut(s) 287, 459
HinP1I GCGC 2 cut(s) 287, 459
HpaII CCGG 5 cut(s) 121, 128, 224, 491, 549
HphI GGTGA 3 cut(s) 425, 488, 563
Hpy188I TCNGA 2 cut(s) 175, 448
Hpy188III TCNNGA 2 cut(s) 425, 491
Hpy99I CGWCG 2 cut(s) 308, 455
HpyAV CCTTC 1 cut(s) 572
HpyCH4III ACNGT 1 cut(s) 437
HpyCH4IV ACGT 2 cut(s) 255, 542
HpyF10VI GCNNNNNNNGC 7 cut(s) 65, 245, 286, 365, 371, 374, 513
HpySE526I ACGT 2 cut(s) 255, 542
Hsp92II CATG 5 cut(s) 10, 516, 531, 573, 591
HspAI GCGC 2 cut(s) 287, 459
Kpn2I TCCGGA 1 cut(s) 490
Ksp22I TGATCA 2 cut(s) 39, 421
Kzo9I GATC 3 cut(s) 39, 421, 427
LmnI GCTCC 4 cut(s) 10, 184, 313, 493
MaeI CTAG 3 cut(s) 212, 345, 405
MaeII ACGT 2 cut(s) 255, 542
MaeIII GTNAC 1 cut(s) 198
MalI GATC 3 cut(s) 41, 423, 429
MboI GATC 3 cut(s) 39, 421, 427
MboII GAAGA 1 cut(s) 41
MhlI GDGCHC 2 cut(s) 231, 318
MlsI TGGCCA 1 cut(s) 574
MluNI TGGCCA 1 cut(s) 574
MmeI TCCRAC 1 cut(s) 515
MnlI CCTC 7 cut(s) 138, 281, 301, 318, 340, 547, 577
Mox20I TGGCCA 1 cut(s) 574
MroI TCCGGA 1 cut(s) 490
MscI TGGCCA 1 cut(s) 574
MseI TTAA 1 cut(s) 566
MslI CAYNNNNRTG 3 cut(s) 435, 438, 568
Msp20I TGGCCA 1 cut(s) 574
MspA1I CMGCKG 3 cut(s) 59, 292, 507
MspI CCGG 5 cut(s) 121, 128, 224, 491, 549
MspR9I CCNGG 2 cut(s) 121, 128
MwoI GCNNNNNNNGC 7 cut(s) 65, 245, 286, 365, 371, 374, 513
NciI CCSGG 2 cut(s) 121, 128
NdeII GATC 3 cut(s) 39, 421, 427
NheI GCTAGC 1 cut(s) 404
NlaIII CATG 5 cut(s) 10, 516, 531, 573, 591
NlaIV GGNNCC 5 cut(s) 118, 125, 186, 217, 228
NmeAIII GCCGAG 1 cut(s) 348
NmuCI GTSAC 1 cut(s) 198
NspI RCATGY 2 cut(s) 516, 591
NspV TTCGAA 1 cut(s) 521
PcsI WCGNNNNNNNCGW 1 cut(s) 459
PpuMI RGGWCCY 1 cut(s) 216
Psp5II RGGWCCY 1 cut(s) 216
PspN4I GGNNCC 5 cut(s) 118, 125, 186, 217, 228
PspPI GGNCC 2 cut(s) 216, 327
PspPPI RGGWCCY 1 cut(s) 216
PvuII CAGCTG 1 cut(s) 292
RsaI GTAC 4 cut(s) 5, 340, 400, 541
RsaNI GTAC 4 cut(s) 4, 339, 399, 540
RseI CAYNNNNRTG 3 cut(s) 435, 438, 568
SaqAI TTAA 1 cut(s) 566
Sau3AI GATC 3 cut(s) 39, 421, 427
Sau96I GGNCC 2 cut(s) 216, 327
ScrFI CCNGG 2 cut(s) 121, 128
SduI GDGCHC 2 cut(s) 231, 318
SetI ASST 9 cut(s) 39, 258, 294, 312, 338, 370, 406, 498, 545
SfuI TTCGAA 1 cut(s) 521
SinI GGWCC 1 cut(s) 216
SmiMI CAYNNNNRTG 3 cut(s) 435, 438, 568
SsiI CCGC 8 cut(s) 57, 63, 151, 154, 260, 280, 359, 507
SspMI CTAG 3 cut(s) 212, 345, 405
StyD4I CCNGG 2 cut(s) 119, 126
StyI CCWWGG 1 cut(s) 243
TaaI ACNGT 1 cut(s) 437
TaiI ACGT 2 cut(s) 258, 545
TaqI TCGA 1 cut(s) 521
TatI WGTACW 2 cut(s) 3, 398
TauI GCSGC 4 cut(s) 65, 154, 263, 362
Tru1I TTAA 1 cut(s) 566
Tru9I TTAA 1 cut(s) 566
TscAI CASTG 2 cut(s) 31, 421
TseFI GTSAC 1 cut(s) 198
Tsp45I GTSAC 1 cut(s) 198
TspDTI ATGAA 3 cut(s) 111, 401, 515
TspRI CASTG 2 cut(s) 31, 421
VpaK11BI GGWCC 1 cut(s) 216
XceI RCATGY 2 cut(s) 516, 591
XspI CTAG 3 cut(s) 212, 345, 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.