Rh1BG253900

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0016007
Physical Location & Seq
Forward (+)
38376322 .. 38378019
1698 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG253900.1

Sequence Viewer

Length: 606 bp
ATGACACTCCCAGCTAATGCTTTCTTAACGTACATGGAGCAACCACCAGACACTGAAGATGACCTGAGCACTCCCACCACCACCACTGCCGCAGCCCCCATCACCCAAAACCACGAAAACCAACAACGCAGCGGCACCCGGCACCCAGTGTACAGGGGCGTGAGGAAGCGGCGGTGGGGAAAATGGGTGTCCGAAATCAGGGAGCCACGCAAGAAGTCACGCATTTGGCTAGGGTCCTTCCCGGTGCCCGAGATGGCGGCCAAGGCCTATGACGTGGCGGCCTACTGCCTCAAGGGGCGAAAAGCGCAGCTGAACTTCCCCGACGAGGTGGAGCACTTGCCGAGGCCCTCCACGTGTACGGCTAGGGATATTCAGGCAGCAGCAGCTAAAGCAGCACAGACAATGAAGTCATCAAGTACAGCTAGAAGCACTGATCAGGATCATCACCGTGATGTATTGGACGGCGGCGGCGTCGATGATTTTTGGGGTGAGATTGAGCTACCGGAGCTAATGAATAGCGGCACGTGCTACTCGAACTCATGTGGGTGGAGTACGTTTACCGGTGATACAGCATTAACATGGCCAGAAGGAGAGGCATGTCTATAA

Protein Analysis

201

Amino Acids

22.32

Weight (kDa)

5.96

Isoelectric Point (pI)

53.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 49 - 99 1.1e-13 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016007)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01250
fragaria_vesca FvH4_7g16810
malus_domestica MD01G1083000.v1.1 MD07G1151700.v1.1
prunus_persica Prupe.2G190200_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0360021
rosa_laevigata RLG00000027838
rosa_multiflora Rmu_ssc0000368.1_g000032
rosa_roxburghii Rroxscaffold_4G00296450
rosa_rugosa Rorug01G0274600
rosa_samantha Rh1AG288400 Rh1BG253900 Rh1CG271400 Rh1DG283000
rosa_wichuraiana Rw1G025570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 406
AccB1I GGYRCC 3 cut(s) 134, 141, 244
AciI CCGC 9 cut(s) 90, 132, 169, 172, 257, 278, 465, 468, 519
AclWI GGATC 1 cut(s) 447
AcoI YGGCCR 2 cut(s) 258, 581
AcuI CTGAAG 1 cut(s) 75
AcvI CACGTG 2 cut(s) 354, 525
AcyI GRCGYC 1 cut(s) 471
AdeI CACNNNGTG 1 cut(s) 148
AfaI GTAC 5 cut(s) 32, 152, 358, 418, 553
AfiI CCNNNNNNNGG 2 cut(s) 198, 325
AflIII ACRYGT 1 cut(s) 353
AgeI ACCGGT 1 cut(s) 560
AjiI CACGTC 1 cut(s) 274
AluBI AGCT 6 cut(s) 14, 310, 386, 422, 499, 508
AluI AGCT 6 cut(s) 14, 310, 386, 422, 499, 508
Alw21I GWGCWC 2 cut(s) 71, 336
AlwI GGATC 1 cut(s) 447
AlwNI CAGNNNCTG 1 cut(s) 53
Ama87I CYCGRG 1 cut(s) 248
AoxI GGCC 5 cut(s) 258, 264, 279, 344, 581
ApeKI GCWGC 7 cut(s) 92, 129, 307, 377, 380, 383, 392
AsiGI ACCGGT 1 cut(s) 560
AspLEI GCGC 1 cut(s) 307
AspS9I GGNCC 2 cut(s) 234, 345
AsuC2I CCSGG 2 cut(s) 139, 242
AsuHPI GGTGA 4 cut(s) 94, 437, 500, 575
AvaI CYCGRG 1 cut(s) 248
AvaII GGWCC 1 cut(s) 234
BaeGI GKGCMC 1 cut(s) 249
BalI TGGCCA 1 cut(s) 583
BanI GGYRCC 3 cut(s) 134, 141, 244
BbrPI CACGTG 2 cut(s) 354, 525
Bbv12I GWGCWC 2 cut(s) 71, 336
BbvI GCAGC 7 cut(s) 104, 141, 319, 389, 392, 395, 404
BccI CCATC 2 cut(s) 107, 247
BceAI ACGGC 2 cut(s) 375, 478
BclI TGATCA 1 cut(s) 433
BcnI CCSGG 2 cut(s) 139, 242
BfaI CTAG 3 cut(s) 230, 363, 423
Bme1390I CCNGG 2 cut(s) 139, 242
Bme18I GGWCC 1 cut(s) 234
BmeT110I CYCGRG 1 cut(s) 248
BmgBI CACGTC 1 cut(s) 274
BmgT120I GGNCC 2 cut(s) 234, 345
BmiI GGNNCC 5 cut(s) 136, 143, 204, 235, 246
BmrFI CCNGG 2 cut(s) 139, 242
BmrI ACTGGG 1 cut(s) 140
BmuI ACTGGG 1 cut(s) 140
Bpu10I CCTNAGC 1 cut(s) 65
BpuEI CTTGAG 1 cut(s) 275
BpuMI CCSGG 2 cut(s) 139, 242
BsaAI YACGTR 2 cut(s) 354, 525
BsaBI GATNNNNATC 1 cut(s) 438
BsaHI GRCGYC 1 cut(s) 471
BsaJI CCNNGG 2 cut(s) 261, 341
BsaWI WCCGGW 2 cut(s) 502, 560
Bsc4I CCNNNNNNNGG 2 cut(s) 198, 325
Bse118I RCCGGY 1 cut(s) 560
Bse1I ACTGG 1 cut(s) 146
Bse8I GATNNNNATC 1 cut(s) 438
BseDI CCNNGG 2 cut(s) 261, 341
BseJI GATNNNNATC 1 cut(s) 438
BseLI CCNNNNNNNGG 2 cut(s) 198, 325
BseMII CTCAG 1 cut(s) 56
BseNI ACTGG 1 cut(s) 146
BseSI GKGCMC 1 cut(s) 249
BseXI GCAGC 7 cut(s) 104, 141, 319, 389, 392, 395, 404
BseYI CCCAGC 1 cut(s) 10
BshFI GGCC 5 cut(s) 260, 266, 281, 346, 583
BshNI GGYRCC 3 cut(s) 134, 141, 244
BshTI ACCGGT 1 cut(s) 560
BsiHKAI GWGCWC 2 cut(s) 71, 336
BsiHKCI CYCGRG 1 cut(s) 248
BsiSI CCGG 4 cut(s) 139, 242, 503, 561
BslI CCNNNNNNNGG 2 cut(s) 198, 325
BsnI GGCC 5 cut(s) 260, 266, 281, 346, 583
BsoBI CYCGRG 1 cut(s) 248
Bsp1286I GDGCHC 3 cut(s) 71, 249, 336
Bsp1407I TGTACA 1 cut(s) 150
Bsp143I GATC 2 cut(s) 433, 439
BspACI CCGC 9 cut(s) 90, 132, 169, 172, 257, 278, 465, 468, 519
BspANI GGCC 5 cut(s) 260, 266, 281, 346, 583
BspCNI CTCAG 1 cut(s) 57
BspLI GGNNCC 5 cut(s) 136, 143, 204, 235, 246
BspPI GGATC 1 cut(s) 447
BspT107I GGYRCC 3 cut(s) 134, 141, 244
BsrFI RCCGGY 1 cut(s) 560
BsrGI TGTACA 1 cut(s) 150
BsrI ACTGG 1 cut(s) 146
BssAI RCCGGY 1 cut(s) 560
BssECI CCNNGG 2 cut(s) 261, 341
BssMI GATC 2 cut(s) 433, 439
BssNI GRCGYC 1 cut(s) 471
BssT1I CCWWGG 1 cut(s) 261
Bst4CI ACNGT 1 cut(s) 449
BstACI GRCGYC 1 cut(s) 471
BstAUI TGTACA 1 cut(s) 150
BstBAI YACGTR 2 cut(s) 354, 525
BstDEI CTNAG 1 cut(s) 65
BstHHI GCGC 1 cut(s) 307
BstKTI GATC 2 cut(s) 436, 442
BstMBI GATC 2 cut(s) 433, 439
BstMWI GCNNNNNNNGC 7 cut(s) 263, 304, 383, 389, 392, 505, 525
BstNSI RCATGY 1 cut(s) 600
BstSCI CCNGG 2 cut(s) 137, 240
BstSLI GKGCMC 1 cut(s) 249
BstV1I GCAGC 7 cut(s) 104, 141, 319, 389, 392, 395, 404
BsuRI GGCC 5 cut(s) 260, 266, 281, 346, 583
BtrI CACGTC 1 cut(s) 274
BtsI GCAGTG 1 cut(s) 84
BtsIMutI CAGTG 4 cut(s) 51, 84, 153, 429
CaiI CAGNNNCTG 1 cut(s) 53
CfoI GCGC 1 cut(s) 307
Cfr10I RCCGGY 1 cut(s) 560
Cfr13I GGNCC 2 cut(s) 234, 345
CseI GACGC 1 cut(s) 460
Csp6I GTAC 5 cut(s) 31, 151, 357, 417, 552
CspAI ACCGGT 1 cut(s) 560
CviAII CATG 4 cut(s) 34, 540, 579, 597
CviQI GTAC 5 cut(s) 31, 151, 357, 417, 552
DdeI CTNAG 1 cut(s) 65
DpnI GATC 2 cut(s) 435, 441
DpnII GATC 2 cut(s) 433, 439
DraIII CACNNNGTG 1 cut(s) 148
DrdI GACNNNNNNGTC 1 cut(s) 406
DseDI GACNNNNNNGTC 1 cut(s) 406
EaeI YGGCCR 2 cut(s) 258, 581
Eco130I CCWWGG 1 cut(s) 261
Eco147I AGGCCT 1 cut(s) 266
Eco47I GGWCC 1 cut(s) 234
Eco57I CTGAAG 1 cut(s) 75
Eco72I CACGTG 2 cut(s) 354, 525
Eco88I CYCGRG 1 cut(s) 248
EcoO109I RGGNCCY 2 cut(s) 234, 345
EcoT14I CCWWGG 1 cut(s) 261
ErhI CCWWGG 1 cut(s) 261
FaeI CATG 4 cut(s) 37, 543, 582, 600
FaiI YATR 6 cut(s) 35, 270, 541, 580, 598, 604
FatI CATG 4 cut(s) 33, 539, 578, 596
FbaI TGATCA 1 cut(s) 433
FspBI CTAG 3 cut(s) 230, 363, 423
GlaI GCGC 1 cut(s) 306
GsaI CCCAGC 1 cut(s) 14
HaeIII GGCC 5 cut(s) 260, 266, 281, 346, 583
HapII CCGG 4 cut(s) 139, 242, 503, 561
HgaI GACGC 1 cut(s) 460
HhaI GCGC 1 cut(s) 307
Hin1I GRCGYC 1 cut(s) 471
Hin1II CATG 4 cut(s) 37, 543, 582, 600
Hin6I GCGC 1 cut(s) 305
HinP1I GCGC 1 cut(s) 305
HpaII CCGG 4 cut(s) 139, 242, 503, 561
HphI GGTGA 4 cut(s) 94, 437, 500, 575
Hpy166II GTNNAC 3 cut(s) 151, 357, 558
Hpy188I TCNGA 1 cut(s) 193
Hpy188III TCNNGA 1 cut(s) 437
Hpy8I GTNNAC 3 cut(s) 151, 357, 558
Hpy99I CGWCG 2 cut(s) 326, 476
HpyAV CCTTC 2 cut(s) 247, 581
HpyCH4III ACNGT 1 cut(s) 449
HpyCH4IV ACGT 5 cut(s) 29, 273, 353, 524, 554
HpyF10VI GCNNNNNNNGC 7 cut(s) 263, 304, 383, 389, 392, 505, 525
HpyF3I CTNAG 1 cut(s) 65
HpySE526I ACGT 5 cut(s) 29, 273, 353, 524, 554
Hsp92I GRCGYC 1 cut(s) 471
Hsp92II CATG 4 cut(s) 37, 543, 582, 600
HspAI GCGC 1 cut(s) 305
Ksp22I TGATCA 1 cut(s) 433
Kzo9I GATC 2 cut(s) 433, 439
LmnI GCTCC 4 cut(s) 37, 202, 331, 505
Lsp1109I GCAGC 7 cut(s) 104, 141, 319, 389, 392, 395, 404
MaeI CTAG 3 cut(s) 230, 363, 423
MaeII ACGT 5 cut(s) 29, 273, 353, 524, 554
MaeIII GTNAC 1 cut(s) 216
MalI GATC 2 cut(s) 435, 441
MboI GATC 2 cut(s) 433, 439
MboII GAAGA 1 cut(s) 68
MhlI GDGCHC 3 cut(s) 71, 249, 336
MlsI TGGCCA 1 cut(s) 583
MluNI TGGCCA 1 cut(s) 583
MnlI CCTC 6 cut(s) 156, 299, 319, 336, 358, 586
Mox20I TGGCCA 1 cut(s) 583
MscI TGGCCA 1 cut(s) 583
MseI TTAA 2 cut(s) 26, 575
MslI CAYNNNNRTG 4 cut(s) 447, 450, 544, 577
Msp20I TGGCCA 1 cut(s) 583
MspA1I CMGCKG 2 cut(s) 132, 310
MspI CCGG 4 cut(s) 139, 242, 503, 561
MspR9I CCNGG 2 cut(s) 139, 242
MwoI GCNNNNNNNGC 7 cut(s) 263, 304, 383, 389, 392, 505, 525
NciI CCSGG 2 cut(s) 139, 242
NdeII GATC 2 cut(s) 433, 439
NlaIII CATG 4 cut(s) 37, 543, 582, 600
NlaIV GGNNCC 5 cut(s) 136, 143, 204, 235, 246
NmeAIII GCCGAG 1 cut(s) 366
NmuCI GTSAC 1 cut(s) 216
NspI RCATGY 1 cut(s) 600
PceI AGGCCT 1 cut(s) 266
PcsI WCGNNNNNNNCGW 2 cut(s) 468, 530
PinAI ACCGGT 1 cut(s) 560
PmaCI CACGTG 2 cut(s) 354, 525
PmlI CACGTG 2 cut(s) 354, 525
Ppu21I YACGTR 2 cut(s) 354, 525
PpuMI RGGWCCY 1 cut(s) 234
Psp5II RGGWCCY 1 cut(s) 234
PspCI CACGTG 2 cut(s) 354, 525
PspFI CCCAGC 1 cut(s) 10
PspN4I GGNNCC 5 cut(s) 136, 143, 204, 235, 246
PspPI GGNCC 2 cut(s) 234, 345
PspPPI RGGWCCY 1 cut(s) 234
PstNI CAGNNNCTG 1 cut(s) 53
PvuII CAGCTG 1 cut(s) 310
RsaI GTAC 5 cut(s) 32, 152, 358, 418, 553
RsaNI GTAC 5 cut(s) 31, 151, 357, 417, 552
RseI CAYNNNNRTG 4 cut(s) 447, 450, 544, 577
SaqAI TTAA 2 cut(s) 26, 575
Sau3AI GATC 2 cut(s) 433, 439
Sau96I GGNCC 2 cut(s) 234, 345
ScrFI CCNGG 2 cut(s) 139, 242
SduI GDGCHC 3 cut(s) 71, 249, 336
SinI GGWCC 1 cut(s) 234
SmiMI CAYNNNNRTG 4 cut(s) 447, 450, 544, 577
SmlI CTYRAG 1 cut(s) 290
SmoI CTYRAG 1 cut(s) 290
SseBI AGGCCT 1 cut(s) 266
SsiI CCGC 9 cut(s) 90, 132, 169, 172, 257, 278, 465, 468, 519
SspMI CTAG 3 cut(s) 230, 363, 423
StuI AGGCCT 1 cut(s) 266
StyD4I CCNGG 2 cut(s) 137, 240
StyI CCWWGG 1 cut(s) 261
TaaI ACNGT 1 cut(s) 449
TaiI ACGT 5 cut(s) 32, 276, 356, 527, 557
TaqI TCGA 2 cut(s) 474, 533
TatI WGTACW 2 cut(s) 150, 416
TauI GCSGC 8 cut(s) 92, 135, 172, 260, 281, 468, 471, 522
Tru1I TTAA 2 cut(s) 26, 575
Tru9I TTAA 2 cut(s) 26, 575
TscAI CASTG 4 cut(s) 58, 91, 153, 436
TseFI GTSAC 1 cut(s) 216
TseI GCWGC 7 cut(s) 92, 129, 307, 377, 380, 383, 392
Tsp45I GTSAC 1 cut(s) 216
TspDTI ATGAA 2 cut(s) 419, 527
TspRI CASTG 4 cut(s) 58, 91, 153, 436
VpaK11BI GGWCC 1 cut(s) 234
XceI RCATGY 1 cut(s) 600
XspI CTAG 3 cut(s) 230, 363, 423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.