MD07G1151700.v1.1

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0016007
Physical Location & Seq
Forward (+)
22122629 .. 22123195
567 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1151700.v1.1.491

Sequence Viewer

Length: 567 bp
ATGGAGCAACCACCGTACACTGAAGATGACCCGAGCACCACCACCACCCTCCCAAAAGCTGACTCACAAGGCGGAGGCACCCGCCACCCGGTGTACCGAGGCGTCCGGAAGCGGAGGTGGGGGAAATGGGTGTCCGAAATTAGGGAACCGCGCAAAAAGTCACGCATTTGGCTAGGCTCTTTCCCTGTGCCGGAGATGGCTGCCAAGGCGTACGACGTTGCCGCCTACTGCCTCAAAGGCCGAAAAGCGCAGCTGAACTTCCCCGAGGAAGTTGACCACTTGCCGAGGCCGTCCACGAGTACTGCTAGGGACATTCAGGCAGCAGCAGCCCAAGCAGCACATACAATCATGAAGTCGTCACGTATCACCAGAAAAAGTGACGATGACGACGATGATCATCATCATCACAGCGGAACGGACGGTGGAGATGATTTTTGGGGAGAGATAGAGTTGCCTGAGCTGATGGATAATAGTGGGTGCTATTGGAACTCATGTGGCTGGAATATGACTACGTTTTCAGGTGATGCAGCGGCGGCGGCATGGCCGGAGAGGCTTGTCTCTGATTAA

Protein Analysis

189

Amino Acids

21.02

Weight (kDa)

5.8

Isoelectric Point (pI)

50.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 30 - 80 1e-13 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016007)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01250
fragaria_vesca FvH4_7g16810
malus_domestica MD01G1083000.v1.1 MD07G1151700.v1.1
prunus_persica Prupe.2G190200_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0360021
rosa_laevigata RLG00000027838
rosa_multiflora Rmu_ssc0000368.1_g000032
rosa_roxburghii Rroxscaffold_4G00296450
rosa_rugosa Rorug01G0274600
rosa_samantha Rh1AG288400 Rh1BG253900 Rh1CG271400 Rh1DG283000
rosa_wichuraiana Rw1G025570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 77
AccII CGCG 1 cut(s) 151
AccIII TCCGGA 1 cut(s) 105
AciI CCGC 9 cut(s) 72, 82, 112, 149, 222, 411, 530, 533, 536
AcoI YGGCCR 1 cut(s) 542
AcuI CTGAAG 1 cut(s) 42
AcyI GRCGYC 1 cut(s) 102
AdeI CACNNNGTG 1 cut(s) 91
AfaI GTAC 4 cut(s) 17, 95, 212, 301
AfiI CCNNNNNNNGG 3 cut(s) 88, 141, 190
AluBI AGCT 3 cut(s) 59, 253, 460
AluI AGCT 3 cut(s) 59, 253, 460
Alw21I GWGCWC 1 cut(s) 38
Alw26I GTCTC 1 cut(s) 562
Ama87I CYCGRG 2 cut(s) 31, 263
Aor13HI TCCGGA 1 cut(s) 105
AoxI GGCC 3 cut(s) 238, 287, 542
ApeKI GCWGC 7 cut(s) 200, 250, 320, 323, 326, 335, 527
AspLEI GCGC 2 cut(s) 153, 250
AsuC2I CCSGG 1 cut(s) 89
AsuHPI GGTGA 2 cut(s) 358, 533
AvaI CYCGRG 2 cut(s) 31, 263
BanI GGYRCC 1 cut(s) 77
BauI CACGAG 1 cut(s) 295
Bbv12I GWGCWC 1 cut(s) 38
BbvI GCAGC 7 cut(s) 187, 262, 332, 335, 338, 347, 539
BccI CCATC 2 cut(s) 190, 457
BceAI ACGGC 1 cut(s) 274
BclI TGATCA 1 cut(s) 394
BcnI CCSGG 1 cut(s) 89
BcoDI GTCTC 1 cut(s) 562
BfaI CTAG 2 cut(s) 173, 306
BglI GCCNNNNNGGC 2 cut(s) 237, 550
BmcAI AGTACT 1 cut(s) 301
Bme1390I CCNGG 1 cut(s) 89
BmeT110I CYCGRG 2 cut(s) 31, 263
BmiI GGNNCC 2 cut(s) 79, 147
BmrFI CCNGG 1 cut(s) 89
BmsI GCATC 1 cut(s) 514
Bpu10I CCTNAGC 1 cut(s) 456
BpuMI CCSGG 1 cut(s) 89
BsaAI YACGTR 1 cut(s) 362
BsaBI GATNNNNATC 2 cut(s) 396, 399
BsaHI GRCGYC 1 cut(s) 102
BsaJI CCNNGG 4 cut(s) 97, 204, 264, 284
BsaWI WCCGGW 1 cut(s) 105
Bsc4I CCNNNNNNNGG 3 cut(s) 88, 141, 190
Bse8I GATNNNNATC 2 cut(s) 396, 399
BseAI TCCGGA 1 cut(s) 105
BseDI CCNNGG 4 cut(s) 97, 204, 264, 284
BseJI GATNNNNATC 2 cut(s) 396, 399
BseLI CCNNNNNNNGG 3 cut(s) 88, 141, 190
BseMII CTCAG 1 cut(s) 447
BseXI GCAGC 7 cut(s) 187, 262, 332, 335, 338, 347, 539
Bsh1236I CGCG 1 cut(s) 151
BshFI GGCC 3 cut(s) 240, 289, 544
BshNI GGYRCC 1 cut(s) 77
BsiHKAI GWGCWC 1 cut(s) 38
BsiHKCI CYCGRG 2 cut(s) 31, 263
BsiSI CCGG 4 cut(s) 89, 106, 191, 545
BsiWI CGTACG 1 cut(s) 210
BslFI GGGAC 1 cut(s) 323
BslI CCNNNNNNNGG 3 cut(s) 88, 141, 190
BsmAI GTCTC 1 cut(s) 562
BsmFI GGGAC 1 cut(s) 323
BsnI GGCC 3 cut(s) 240, 289, 544
BsoBI CYCGRG 2 cut(s) 31, 263
Bsp1286I GDGCHC 1 cut(s) 38
Bsp13I TCCGGA 1 cut(s) 105
Bsp143I GATC 1 cut(s) 394
BspACI CCGC 9 cut(s) 72, 82, 112, 149, 222, 411, 530, 533, 536
BspANI GGCC 3 cut(s) 240, 289, 544
BspCNI CTCAG 1 cut(s) 448
BspEI TCCGGA 1 cut(s) 105
BspFNI CGCG 1 cut(s) 151
BspHI TCATGA 1 cut(s) 348
BspLI GGNNCC 2 cut(s) 79, 147
BspT107I GGYRCC 1 cut(s) 77
BssECI CCNNGG 4 cut(s) 97, 204, 264, 284
BssMI GATC 1 cut(s) 394
BssNI GRCGYC 1 cut(s) 102
BssSI CACGAG 1 cut(s) 295
BssT1I CCWWGG 1 cut(s) 204
Bst2BI CACGAG 1 cut(s) 295
Bst4CI ACNGT 2 cut(s) 15, 422
BstACI GRCGYC 1 cut(s) 102
BstBAI YACGTR 1 cut(s) 362
BstDEI CTNAG 1 cut(s) 456
BstFNI CGCG 1 cut(s) 151
BstHHI GCGC 2 cut(s) 153, 250
BstKTI GATC 1 cut(s) 397
BstMAI GTCTC 1 cut(s) 562
BstMBI GATC 1 cut(s) 394
BstMWI GCNNNNNNNGC 8 cut(s) 206, 237, 326, 332, 335, 533, 536, 550
BstSCI CCNGG 1 cut(s) 87
BstUI CGCG 1 cut(s) 151
BstV1I GCAGC 7 cut(s) 187, 262, 332, 335, 338, 347, 539
BsuRI GGCC 3 cut(s) 240, 289, 544
BtsIMutI CAGTG 1 cut(s) 18
CciI TCATGA 1 cut(s) 348
CfoI GCGC 2 cut(s) 153, 250
CseI GACGC 1 cut(s) 91
Csp6I GTAC 4 cut(s) 16, 94, 211, 300
CviAII CATG 3 cut(s) 349, 492, 540
CviQI GTAC 4 cut(s) 16, 94, 211, 300
DdeI CTNAG 1 cut(s) 456
DpnI GATC 1 cut(s) 396
DpnII GATC 1 cut(s) 394
DraIII CACNNNGTG 1 cut(s) 91
EaeI YGGCCR 1 cut(s) 542
EciI GGCGGA 1 cut(s) 87
Eco130I CCWWGG 1 cut(s) 204
Eco57I CTGAAG 1 cut(s) 42
Eco88I CYCGRG 2 cut(s) 31, 263
EcoT14I CCWWGG 1 cut(s) 204
ErhI CCWWGG 1 cut(s) 204
FaeI CATG 3 cut(s) 352, 495, 543
FaiI YATR 5 cut(s) 342, 350, 493, 506, 541
FaqI GGGAC 1 cut(s) 323
FatI CATG 3 cut(s) 348, 491, 539
FauI CCCGC 1 cut(s) 89
FbaI TGATCA 1 cut(s) 394
FspBI CTAG 2 cut(s) 173, 306
GlaI GCGC 2 cut(s) 152, 249
HaeIII GGCC 3 cut(s) 240, 289, 544
HapII CCGG 4 cut(s) 89, 106, 191, 545
HgaI GACGC 1 cut(s) 91
HhaI GCGC 2 cut(s) 153, 250
Hin1I GRCGYC 1 cut(s) 102
Hin1II CATG 3 cut(s) 352, 495, 543
Hin6I GCGC 2 cut(s) 151, 248
HinP1I GCGC 2 cut(s) 151, 248
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HinfI GANTC 1 cut(s) 62
HpaII CCGG 4 cut(s) 89, 106, 191, 545
HphI GGTGA 2 cut(s) 358, 533
Hpy166II GTNNAC 4 cut(s) 18, 94, 274, 294
Hpy188I TCNGA 2 cut(s) 136, 562
Hpy188III TCNNGA 2 cut(s) 106, 349
Hpy8I GTNNAC 4 cut(s) 18, 94, 274, 294
Hpy99I CGWCG 2 cut(s) 218, 392
HpyCH4III ACNGT 2 cut(s) 15, 422
HpyCH4IV ACGT 3 cut(s) 216, 361, 512
HpyCH4V TGCA 1 cut(s) 527
HpyF10VI GCNNNNNNNGC 8 cut(s) 206, 237, 326, 332, 335, 533, 536, 550
HpyF3I CTNAG 1 cut(s) 456
HpySE526I ACGT 3 cut(s) 216, 361, 512
Hsp92I GRCGYC 1 cut(s) 102
Hsp92II CATG 3 cut(s) 352, 495, 543
HspAI GCGC 2 cut(s) 151, 248
Kpn2I TCCGGA 1 cut(s) 105
Ksp22I TGATCA 1 cut(s) 394
Kzo9I GATC 1 cut(s) 394
LmnI GCTCC 1 cut(s) 4
Lsp1109I GCAGC 7 cut(s) 187, 262, 332, 335, 338, 347, 539
LweI GCATC 1 cut(s) 514
MaeI CTAG 2 cut(s) 173, 306
MaeII ACGT 3 cut(s) 216, 361, 512
MaeIII GTNAC 3 cut(s) 159, 357, 377
MalI GATC 1 cut(s) 396
MboI GATC 1 cut(s) 394
MboII GAAGA 1 cut(s) 35
MhlI GDGCHC 1 cut(s) 38
MluCI AATT 1 cut(s) 138
MlyI GAGTC 1 cut(s) 56
MnlI CCTC 8 cut(s) 59, 68, 92, 108, 242, 259, 279, 543
MroI TCCGGA 1 cut(s) 105
MseI TTAA 1 cut(s) 565
MspA1I CMGCKG 3 cut(s) 253, 411, 530
MspI CCGG 4 cut(s) 89, 106, 191, 545
MspR9I CCNGG 1 cut(s) 89
MvnI CGCG 1 cut(s) 151
MwoI GCNNNNNNNGC 8 cut(s) 206, 237, 326, 332, 335, 533, 536, 550
NciI CCSGG 1 cut(s) 89
NdeII GATC 1 cut(s) 394
NlaIII CATG 3 cut(s) 352, 495, 543
NlaIV GGNNCC 2 cut(s) 79, 147
NmeAIII GCCGAG 1 cut(s) 309
NmuCI GTSAC 3 cut(s) 159, 357, 377
PagI TCATGA 1 cut(s) 348
PcsI WCGNNNNNNNCGW 1 cut(s) 387
Pfl23II CGTACG 1 cut(s) 210
PleI GAGTC 1 cut(s) 56
PpsI GAGTC 1 cut(s) 56
Ppu21I YACGTR 1 cut(s) 362
PspLI CGTACG 1 cut(s) 210
PspN4I GGNNCC 2 cut(s) 79, 147
PvuII CAGCTG 1 cut(s) 253
RsaI GTAC 4 cut(s) 17, 95, 212, 301
RsaNI GTAC 4 cut(s) 16, 94, 211, 300
SaqAI TTAA 1 cut(s) 565
Sau3AI GATC 1 cut(s) 394
ScaI AGTACT 1 cut(s) 301
SchI GAGTC 1 cut(s) 56
ScrFI CCNGG 1 cut(s) 89
SduI GDGCHC 1 cut(s) 38
SetI ASST 8 cut(s) 61, 119, 219, 255, 364, 462, 515, 523
SfaNI GCATC 1 cut(s) 514
Sse9I AATT 1 cut(s) 138
SsiI CCGC 9 cut(s) 72, 82, 112, 149, 222, 411, 530, 533, 536
SspMI CTAG 2 cut(s) 173, 306
StyD4I CCNGG 1 cut(s) 87
StyI CCWWGG 1 cut(s) 204
TaaI ACNGT 2 cut(s) 15, 422
TaiI ACGT 3 cut(s) 219, 364, 515
TasI AATT 1 cut(s) 138
TatI WGTACW 1 cut(s) 299
TauI GCSGC 4 cut(s) 224, 533, 536, 539
Tru1I TTAA 1 cut(s) 565
Tru9I TTAA 1 cut(s) 565
TscAI CASTG 1 cut(s) 25
TseFI GTSAC 3 cut(s) 159, 357, 377
TseI GCWGC 7 cut(s) 200, 250, 320, 323, 326, 335, 527
Tsp45I GTSAC 3 cut(s) 159, 357, 377
TspDTI ATGAA 1 cut(s) 365
TspGWI ACGGA 1 cut(s) 431
TspRI CASTG 1 cut(s) 25
XspI CTAG 2 cut(s) 173, 306
ZrmI AGTACT 1 cut(s) 301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.