Rroxscaffold_4G00296450

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0016007
Physical Location & Seq
Reverse (-)
16523236 .. 16524670
1435 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00296450.1

Sequence Viewer

Length: 573 bp
ATGGAGCAACCACCAGACACTGTAGATGACCTGAGCACTCCCACCACCACCACTGCCGCAGCCACCATCACCCAAAACCACGAAAACCAACAACGCAGCGGCACCCGGCACCCGGTGTACCGGGGCGTGAGGAAGCGGCGGTGGGGAAAATGGGTGTCCGAAATCAGGGAGCCACGCAAGAAGTCACGCATTTGGCTAGGGTCCTTCCCGGTGCCCGAGATGGCGGCCAAGGCCTATGACGTGGCGGCCTACTGCCTCAAGGGCCGAAAAGCGCAGCTGAACTTCCCCGACGAGGTGGAGCACTTGCCGAGGCCCTCCACGTGTACCGCTAGGGATATTCAGGCAGCAGCAGCTAAAGCAGCACAGACAATGAAATCGTCAAGTACAGCTAGAAGCACTGATCAGGATAATCACCGTGATGTATTGGACGGCGGTGGCGTCGATGATTTTTGGGGTGAGATTGAGCTACCGGAGCTAATGAATAGCGGCACGTGCTACTCGAACTCATGTGGGTGGAGTACGTTTTCCGGTGAGACAGCATTAACATGGCCAGAAGGAGAGGCATGTCTATAA

Protein Analysis

190

Amino Acids

21.05

Weight (kDa)

6.1

Isoelectric Point (pI)

48.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 38 - 88 1e-13 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016007)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01250
fragaria_vesca FvH4_7g16810
malus_domestica MD01G1083000.v1.1 MD07G1151700.v1.1
prunus_persica Prupe.2G190200_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0360021
rosa_laevigata RLG00000027838
rosa_multiflora Rmu_ssc0000368.1_g000032
rosa_roxburghii Rroxscaffold_4G00296450
rosa_rugosa Rorug01G0274600
rosa_samantha Rh1AG288400 Rh1BG253900 Rh1CG271400 Rh1DG283000
rosa_wichuraiana Rw1G025570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 101, 108, 211
AciI CCGC 9 cut(s) 57, 99, 136, 139, 224, 245, 327, 432, 486
AcoI YGGCCR 2 cut(s) 225, 548
AcvI CACGTG 2 cut(s) 321, 492
AcyI GRCGYC 1 cut(s) 438
AdeI CACNNNGTG 1 cut(s) 115
AfaI GTAC 4 cut(s) 119, 325, 385, 520
AfiI CCNNNNNNNGG 3 cut(s) 112, 165, 292
AflIII ACRYGT 1 cut(s) 320
AjiI CACGTC 1 cut(s) 241
AluBI AGCT 5 cut(s) 277, 353, 389, 466, 475
AluI AGCT 5 cut(s) 277, 353, 389, 466, 475
Alw21I GWGCWC 2 cut(s) 38, 303
Alw26I GTCTC 1 cut(s) 527
AlwNI CAGNNNCTG 1 cut(s) 20
Ama87I CYCGRG 1 cut(s) 215
AoxI GGCC 6 cut(s) 225, 231, 246, 262, 311, 548
ApeKI GCWGC 7 cut(s) 59, 96, 274, 344, 347, 350, 359
AspLEI GCGC 1 cut(s) 274
AspS9I GGNCC 3 cut(s) 201, 262, 312
AsuC2I CCSGG 4 cut(s) 106, 113, 122, 209
AsuHPI GGTGA 4 cut(s) 61, 404, 467, 542
AvaI CYCGRG 1 cut(s) 215
AvaII GGWCC 1 cut(s) 201
BaeGI GKGCMC 1 cut(s) 216
BalI TGGCCA 1 cut(s) 550
BanI GGYRCC 3 cut(s) 101, 108, 211
BbrPI CACGTG 2 cut(s) 321, 492
Bbv12I GWGCWC 2 cut(s) 38, 303
BbvI GCAGC 7 cut(s) 71, 108, 286, 356, 359, 362, 371
BccI CCATC 2 cut(s) 74, 214
BceAI ACGGC 1 cut(s) 445
BclI TGATCA 1 cut(s) 400
BcnI CCSGG 4 cut(s) 106, 113, 122, 209
BcoDI GTCTC 1 cut(s) 527
BfaI CTAG 3 cut(s) 197, 330, 390
BfmI CTRYAG 1 cut(s) 21
BglI GCCNNNNNGGC 1 cut(s) 261
Bme1390I CCNGG 4 cut(s) 106, 113, 122, 209
Bme18I GGWCC 1 cut(s) 201
BmeT110I CYCGRG 1 cut(s) 215
BmgBI CACGTC 1 cut(s) 241
BmgT120I GGNCC 3 cut(s) 201, 262, 312
BmiI GGNNCC 5 cut(s) 103, 110, 171, 202, 213
BmrFI CCNGG 4 cut(s) 106, 113, 122, 209
Bpu10I CCTNAGC 1 cut(s) 32
BpuEI CTTGAG 1 cut(s) 242
BpuMI CCSGG 4 cut(s) 106, 113, 122, 209
BsaAI YACGTR 2 cut(s) 321, 492
BsaHI GRCGYC 1 cut(s) 438
BsaJI CCNNGG 3 cut(s) 121, 228, 308
BsaWI WCCGGW 2 cut(s) 469, 527
Bsc4I CCNNNNNNNGG 3 cut(s) 112, 165, 292
BseDI CCNNGG 3 cut(s) 121, 228, 308
BseLI CCNNNNNNNGG 3 cut(s) 112, 165, 292
BseMII CTCAG 1 cut(s) 23
BseSI GKGCMC 1 cut(s) 216
BseXI GCAGC 7 cut(s) 71, 108, 286, 356, 359, 362, 371
BshFI GGCC 6 cut(s) 227, 233, 248, 264, 313, 550
BshNI GGYRCC 3 cut(s) 101, 108, 211
BsiHKAI GWGCWC 2 cut(s) 38, 303
BsiHKCI CYCGRG 1 cut(s) 215
BsiSI CCGG 6 cut(s) 106, 113, 121, 209, 470, 528
BslI CCNNNNNNNGG 3 cut(s) 112, 165, 292
BsmAI GTCTC 1 cut(s) 527
BsnI GGCC 6 cut(s) 227, 233, 248, 264, 313, 550
BsoBI CYCGRG 1 cut(s) 215
Bsp1286I GDGCHC 3 cut(s) 38, 216, 303
Bsp143I GATC 1 cut(s) 400
BspACI CCGC 9 cut(s) 57, 99, 136, 139, 224, 245, 327, 432, 486
BspANI GGCC 6 cut(s) 227, 233, 248, 264, 313, 550
BspCNI CTCAG 1 cut(s) 24
BspLI GGNNCC 5 cut(s) 103, 110, 171, 202, 213
BspT107I GGYRCC 3 cut(s) 101, 108, 211
BssECI CCNNGG 3 cut(s) 121, 228, 308
BssMI GATC 1 cut(s) 400
BssNI GRCGYC 1 cut(s) 438
BssT1I CCWWGG 1 cut(s) 228
Bst4CI ACNGT 2 cut(s) 22, 416
BstACI GRCGYC 1 cut(s) 438
BstBAI YACGTR 2 cut(s) 321, 492
BstDEI CTNAG 1 cut(s) 32
BstHHI GCGC 1 cut(s) 274
BstKTI GATC 1 cut(s) 403
BstMAI GTCTC 1 cut(s) 527
BstMBI GATC 1 cut(s) 400
BstMWI GCNNNNNNNGC 7 cut(s) 230, 261, 350, 356, 359, 472, 492
BstNSI RCATGY 1 cut(s) 567
BstSCI CCNGG 4 cut(s) 104, 111, 120, 207
BstSFI CTRYAG 1 cut(s) 21
BstSLI GKGCMC 1 cut(s) 216
BstV1I GCAGC 7 cut(s) 71, 108, 286, 356, 359, 362, 371
BsuRI GGCC 6 cut(s) 227, 233, 248, 264, 313, 550
BtrI CACGTC 1 cut(s) 241
BtsI GCAGTG 1 cut(s) 51
BtsIMutI CAGTG 3 cut(s) 18, 51, 396
CaiI CAGNNNCTG 1 cut(s) 20
CfoI GCGC 1 cut(s) 274
Cfr13I GGNCC 3 cut(s) 201, 262, 312
CseI GACGC 1 cut(s) 427
Csp6I GTAC 4 cut(s) 118, 324, 384, 519
CviAII CATG 3 cut(s) 507, 546, 564
CviQI GTAC 4 cut(s) 118, 324, 384, 519
DdeI CTNAG 1 cut(s) 32
DpnI GATC 1 cut(s) 402
DpnII GATC 1 cut(s) 400
DraIII CACNNNGTG 1 cut(s) 115
EaeI YGGCCR 2 cut(s) 225, 548
Eco130I CCWWGG 1 cut(s) 228
Eco147I AGGCCT 1 cut(s) 233
Eco47I GGWCC 1 cut(s) 201
Eco72I CACGTG 2 cut(s) 321, 492
Eco88I CYCGRG 1 cut(s) 215
EcoO109I RGGNCCY 2 cut(s) 201, 312
EcoT14I CCWWGG 1 cut(s) 228
ErhI CCWWGG 1 cut(s) 228
FaeI CATG 3 cut(s) 510, 549, 567
FaiI YATR 5 cut(s) 237, 508, 547, 565, 571
FatI CATG 3 cut(s) 506, 545, 563
FbaI TGATCA 1 cut(s) 400
FspBI CTAG 3 cut(s) 197, 330, 390
GlaI GCGC 1 cut(s) 273
HaeIII GGCC 6 cut(s) 227, 233, 248, 264, 313, 550
HapII CCGG 6 cut(s) 106, 113, 121, 209, 470, 528
HgaI GACGC 1 cut(s) 427
HhaI GCGC 1 cut(s) 274
Hin1I GRCGYC 1 cut(s) 438
Hin1II CATG 3 cut(s) 510, 549, 567
Hin6I GCGC 1 cut(s) 272
HinP1I GCGC 1 cut(s) 272
HpaII CCGG 6 cut(s) 106, 113, 121, 209, 470, 528
HphI GGTGA 4 cut(s) 61, 404, 467, 542
Hpy166II GTNNAC 2 cut(s) 118, 324
Hpy188I TCNGA 1 cut(s) 160
Hpy188III TCNNGA 1 cut(s) 404
Hpy8I GTNNAC 2 cut(s) 118, 324
Hpy99I CGWCG 2 cut(s) 293, 443
HpyAV CCTTC 2 cut(s) 214, 548
HpyCH4III ACNGT 2 cut(s) 22, 416
HpyCH4IV ACGT 4 cut(s) 240, 320, 491, 521
HpyF10VI GCNNNNNNNGC 7 cut(s) 230, 261, 350, 356, 359, 472, 492
HpyF3I CTNAG 1 cut(s) 32
HpySE526I ACGT 4 cut(s) 240, 320, 491, 521
Hsp92I GRCGYC 1 cut(s) 438
Hsp92II CATG 3 cut(s) 510, 549, 567
HspAI GCGC 1 cut(s) 272
Ksp22I TGATCA 1 cut(s) 400
Kzo9I GATC 1 cut(s) 400
LmnI GCTCC 4 cut(s) 4, 169, 298, 472
Lsp1109I GCAGC 7 cut(s) 71, 108, 286, 356, 359, 362, 371
MaeI CTAG 3 cut(s) 197, 330, 390
MaeII ACGT 4 cut(s) 240, 320, 491, 521
MaeIII GTNAC 1 cut(s) 183
MalI GATC 1 cut(s) 402
MboI GATC 1 cut(s) 400
MhlI GDGCHC 3 cut(s) 38, 216, 303
MlsI TGGCCA 1 cut(s) 550
MluNI TGGCCA 1 cut(s) 550
MnlI CCTC 6 cut(s) 123, 266, 286, 303, 325, 553
Mox20I TGGCCA 1 cut(s) 550
MscI TGGCCA 1 cut(s) 550
MseI TTAA 1 cut(s) 542
MslI CAYNNNNRTG 3 cut(s) 417, 511, 544
Msp20I TGGCCA 1 cut(s) 550
MspA1I CMGCKG 2 cut(s) 99, 277
MspI CCGG 6 cut(s) 106, 113, 121, 209, 470, 528
MspR9I CCNGG 4 cut(s) 106, 113, 122, 209
MwoI GCNNNNNNNGC 7 cut(s) 230, 261, 350, 356, 359, 472, 492
NciI CCSGG 4 cut(s) 106, 113, 122, 209
NdeII GATC 1 cut(s) 400
NlaIII CATG 3 cut(s) 510, 549, 567
NlaIV GGNNCC 5 cut(s) 103, 110, 171, 202, 213
NmeAIII GCCGAG 1 cut(s) 333
NmuCI GTSAC 1 cut(s) 183
NspI RCATGY 1 cut(s) 567
PceI AGGCCT 1 cut(s) 233
PcsI WCGNNNNNNNCGW 2 cut(s) 435, 497
PmaCI CACGTG 2 cut(s) 321, 492
PmlI CACGTG 2 cut(s) 321, 492
Ppu21I YACGTR 2 cut(s) 321, 492
PpuMI RGGWCCY 1 cut(s) 201
Psp5II RGGWCCY 1 cut(s) 201
PspCI CACGTG 2 cut(s) 321, 492
PspN4I GGNNCC 5 cut(s) 103, 110, 171, 202, 213
PspPI GGNCC 3 cut(s) 201, 262, 312
PspPPI RGGWCCY 1 cut(s) 201
PstNI CAGNNNCTG 1 cut(s) 20
PvuII CAGCTG 1 cut(s) 277
RsaI GTAC 4 cut(s) 119, 325, 385, 520
RsaNI GTAC 4 cut(s) 118, 324, 384, 519
RseI CAYNNNNRTG 3 cut(s) 417, 511, 544
SaqAI TTAA 1 cut(s) 542
Sau3AI GATC 1 cut(s) 400
Sau96I GGNCC 3 cut(s) 201, 262, 312
ScrFI CCNGG 4 cut(s) 106, 113, 122, 209
SduI GDGCHC 3 cut(s) 38, 216, 303
SfcI CTRYAG 1 cut(s) 21
SinI GGWCC 1 cut(s) 201
SmiMI CAYNNNNRTG 3 cut(s) 417, 511, 544
SmlI CTYRAG 1 cut(s) 257
SmoI CTYRAG 1 cut(s) 257
SseBI AGGCCT 1 cut(s) 233
SsiI CCGC 9 cut(s) 57, 99, 136, 139, 224, 245, 327, 432, 486
SspMI CTAG 3 cut(s) 197, 330, 390
StuI AGGCCT 1 cut(s) 233
StyD4I CCNGG 4 cut(s) 104, 111, 120, 207
StyI CCWWGG 1 cut(s) 228
TaaI ACNGT 2 cut(s) 22, 416
TaiI ACGT 4 cut(s) 243, 323, 494, 524
TaqI TCGA 2 cut(s) 441, 500
TatI WGTACW 1 cut(s) 383
TauI GCSGC 6 cut(s) 59, 102, 139, 227, 248, 489
Tru1I TTAA 1 cut(s) 542
Tru9I TTAA 1 cut(s) 542
TscAI CASTG 3 cut(s) 25, 58, 403
TseFI GTSAC 1 cut(s) 183
TseI GCWGC 7 cut(s) 59, 96, 274, 344, 347, 350, 359
Tsp45I GTSAC 1 cut(s) 183
TspDTI ATGAA 2 cut(s) 386, 494
TspRI CASTG 3 cut(s) 25, 58, 403
VpaK11BI GGWCC 1 cut(s) 201
XceI RCATGY 1 cut(s) 567
XspI CTAG 3 cut(s) 197, 330, 390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.