MD01G1083000.v1.1

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0016007
Physical Location & Seq
Reverse (-)
18937339 .. 18937899
561 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1083000.v1.1.491

Sequence Viewer

Length: 561 bp
ATGGAACAACCACCATACACTGAAGATGACCAGAGCACCACCACCATCAACACCCAAAAAGCCGACTCGCAAGGTGGAGGCACCCGCCACCCGGTGTACCGAGGCGTCCGGAAGCGGAGGTGGGGGAAATGGGTGTCCGAAATTAGGGAACCGCGCAAGAAGTCACGCATTTGGCTAGGTTCTTTCCCCGTGCCCGAGATGGCAGCCAAGGCCTACGACGTCGCCGCCTACTGTCTCAAAGGCCGAAAAGCACAGCTGAACTTCCCCGAGGACGTCGACCACTTGCCCAGGCCCGCCACGAGTACGGCTAGGGACATTCAGGCAGCAGCAGCCCAAGCAGCACATAGAGTCATCAAGTCATCACACATCACCAAGAAAAATGACGACGACGACGATCATCACGGTGGATTGGACGGTGGCGATGATTTTTGGGGAGAGATTGAGTTGCCTGAGTTGATGAACAAAAGCGGGTGCTATTGGAATTCATGTGGCTGGAATATGACTGCGTTTTCAGGTGATGCAGCGGCGGTAGCATGTCCGGAGGGAGAGGCTTGCCTCTGA

Protein Analysis

187

Amino Acids

20.59

Weight (kDa)

6.07

Isoelectric Point (pI)

58.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 31 - 81 9.9e-14 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016007)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01250
fragaria_vesca FvH4_7g16810
malus_domestica MD01G1083000.v1.1 MD07G1151700.v1.1
prunus_persica Prupe.2G190200_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0360021
rosa_laevigata RLG00000027838
rosa_multiflora Rmu_ssc0000368.1_g000032
rosa_roxburghii Rroxscaffold_4G00296450
rosa_rugosa Rorug01G0274600
rosa_samantha Rh1AG288400 Rh1BG253900 Rh1CG271400 Rh1DG283000
rosa_wichuraiana Rw1G025570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 222, 276
AccB1I GGYRCC 1 cut(s) 80
AccI GTMKAC 1 cut(s) 276
AccII CGCG 1 cut(s) 154
AccIII TCCGGA 2 cut(s) 108, 538
AciI CCGC 8 cut(s) 85, 115, 152, 225, 294, 468, 524, 527
AcsI RAATTY 1 cut(s) 481
AcuI CTGAAG 1 cut(s) 42
AcyI GRCGYC 3 cut(s) 105, 219, 273
AdeI CACNNNGTG 1 cut(s) 94
AfaI GTAC 2 cut(s) 98, 304
AfiI CCNNNNNNNGG 2 cut(s) 91, 144
AjnI CCWGG 1 cut(s) 287
AluBI AGCT 1 cut(s) 256
AluI AGCT 1 cut(s) 256
Alw21I GWGCWC 1 cut(s) 38
Alw26I GTCTC 1 cut(s) 239
Ama87I CYCGRG 2 cut(s) 194, 266
Aor13HI TCCGGA 2 cut(s) 108, 538
AoxI GGCC 3 cut(s) 210, 241, 290
ApeKI GCWGC 6 cut(s) 203, 323, 326, 329, 338, 521
ApoI RAATTY 1 cut(s) 481
AspLEI GCGC 1 cut(s) 156
AspS9I GGNCC 1 cut(s) 291
AsuC2I CCSGG 1 cut(s) 92
AsuHPI GGTGA 2 cut(s) 361, 527
AvaI CYCGRG 2 cut(s) 194, 266
BaeGI GKGCMC 1 cut(s) 195
BanI GGYRCC 1 cut(s) 80
BauI CACGAG 1 cut(s) 298
Bbv12I GWGCWC 1 cut(s) 38
BbvI GCAGC 6 cut(s) 215, 335, 338, 341, 350, 533
BccI CCATC 2 cut(s) 53, 193
BceAI ACGGC 1 cut(s) 321
BciT130I CCWGG 1 cut(s) 289
BcnI CCSGG 1 cut(s) 92
BcoDI GTCTC 1 cut(s) 239
BfaI CTAG 2 cut(s) 176, 309
BisI GCNGC 8 cut(s) 204, 225, 324, 327, 330, 339, 522, 525
BlsI GCNGC 8 cut(s) 205, 226, 325, 328, 331, 340, 523, 526
Bme1390I CCNGG 2 cut(s) 92, 289
BmeT110I CYCGRG 2 cut(s) 194, 266
BmgT120I GGNCC 1 cut(s) 291
BmiI GGNNCC 2 cut(s) 82, 150
BmrFI CCNGG 2 cut(s) 92, 289
BmsI GCATC 1 cut(s) 508
BpuMI CCSGG 1 cut(s) 92
BsaHI GRCGYC 3 cut(s) 105, 219, 273
BsaJI CCNNGG 4 cut(s) 100, 207, 267, 287
BsaWI WCCGGW 2 cut(s) 108, 538
Bsc4I CCNNNNNNNGG 2 cut(s) 91, 144
BseAI TCCGGA 2 cut(s) 108, 538
BseBI CCWGG 1 cut(s) 289
BseDI CCNNGG 4 cut(s) 100, 207, 267, 287
BseLI CCNNNNNNNGG 2 cut(s) 91, 144
BseMII CTCAG 1 cut(s) 441
BseSI GKGCMC 1 cut(s) 195
BseXI GCAGC 6 cut(s) 215, 335, 338, 341, 350, 533
Bsh1236I CGCG 1 cut(s) 154
BshFI GGCC 3 cut(s) 212, 243, 292
BshNI GGYRCC 1 cut(s) 80
BsiHKAI GWGCWC 1 cut(s) 38
BsiHKCI CYCGRG 2 cut(s) 194, 266
BsiSI CCGG 3 cut(s) 92, 109, 539
BslFI GGGAC 1 cut(s) 326
BslI CCNNNNNNNGG 2 cut(s) 91, 144
BsmAI GTCTC 1 cut(s) 239
BsmFI GGGAC 1 cut(s) 326
BsnI GGCC 3 cut(s) 212, 243, 292
BsoBI CYCGRG 2 cut(s) 194, 266
Bsp1286I GDGCHC 2 cut(s) 38, 195
Bsp13I TCCGGA 2 cut(s) 108, 538
Bsp143I GATC 1 cut(s) 394
BspACI CCGC 8 cut(s) 85, 115, 152, 225, 294, 468, 524, 527
BspANI GGCC 3 cut(s) 212, 243, 292
BspCNI CTCAG 1 cut(s) 442
BspEI TCCGGA 2 cut(s) 108, 538
BspFNI CGCG 1 cut(s) 154
BspLI GGNNCC 2 cut(s) 82, 150
BspT107I GGYRCC 1 cut(s) 80
BssECI CCNNGG 4 cut(s) 100, 207, 267, 287
BssMI GATC 1 cut(s) 394
BssNI GRCGYC 3 cut(s) 105, 219, 273
BssSI CACGAG 1 cut(s) 298
BssT1I CCWWGG 1 cut(s) 207
Bst2BI CACGAG 1 cut(s) 298
Bst2UI CCWGG 1 cut(s) 289
Bst4CI ACNGT 3 cut(s) 233, 404, 416
BstACI GRCGYC 3 cut(s) 105, 219, 273
BstC8I GCNNGC 2 cut(s) 294, 553
BstDEI CTNAG 1 cut(s) 450
BstFNI CGCG 1 cut(s) 154
BstHHI GCGC 1 cut(s) 156
BstKTI GATC 1 cut(s) 397
BstMAI GTCTC 1 cut(s) 239
BstMBI GATC 1 cut(s) 394
BstMWI GCNNNNNNNGC 5 cut(s) 209, 329, 335, 338, 530
BstNI CCWGG 1 cut(s) 289
BstNSI RCATGY 1 cut(s) 537
BstSCI CCNGG 2 cut(s) 90, 287
BstSLI GKGCMC 1 cut(s) 195
BstUI CGCG 1 cut(s) 154
BstV1I GCAGC 6 cut(s) 215, 335, 338, 341, 350, 533
BsuRI GGCC 3 cut(s) 212, 243, 292
BtgZI GCGATG 1 cut(s) 435
BtsIMutI CAGTG 1 cut(s) 18
Cac8I GCNNGC 2 cut(s) 294, 553
CfoI GCGC 1 cut(s) 156
Cfr13I GGNCC 1 cut(s) 291
CseI GACGC 1 cut(s) 94
Csp6I GTAC 2 cut(s) 97, 303
CviAII CATG 2 cut(s) 486, 534
CviQI GTAC 2 cut(s) 97, 303
DdeI CTNAG 1 cut(s) 450
DpnI GATC 1 cut(s) 396
DpnII GATC 1 cut(s) 394
DraIII CACNNNGTG 1 cut(s) 94
Eco130I CCWWGG 1 cut(s) 207
Eco147I AGGCCT 1 cut(s) 212
Eco57I CTGAAG 1 cut(s) 42
Eco88I CYCGRG 2 cut(s) 194, 266
EcoRI GAATTC 1 cut(s) 481
EcoRII CCWGG 1 cut(s) 287
EcoT14I CCWWGG 1 cut(s) 207
ErhI CCWWGG 1 cut(s) 207
FaeI CATG 2 cut(s) 489, 537
FaiI YATR 5 cut(s) 16, 345, 487, 500, 535
FaqI GGGAC 1 cut(s) 326
FatI CATG 2 cut(s) 485, 533
FauI CCCGC 3 cut(s) 92, 301, 461
FblI GTMKAC 1 cut(s) 276
Fnu4HI GCNGC 8 cut(s) 204, 225, 324, 327, 330, 339, 522, 525
Fsp4HI GCNGC 8 cut(s) 204, 225, 324, 327, 330, 339, 522, 525
FspBI CTAG 2 cut(s) 176, 309
GlaI GCGC 1 cut(s) 155
GluI GCNGC 8 cut(s) 204, 225, 324, 327, 330, 339, 522, 525
HaeIII GGCC 3 cut(s) 212, 243, 292
HapII CCGG 3 cut(s) 92, 109, 539
HgaI GACGC 1 cut(s) 94
HhaI GCGC 1 cut(s) 156
Hin1I GRCGYC 3 cut(s) 105, 219, 273
Hin1II CATG 2 cut(s) 489, 537
Hin6I GCGC 1 cut(s) 154
HinP1I GCGC 1 cut(s) 154
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HinfI GANTC 2 cut(s) 65, 348
HpaII CCGG 3 cut(s) 92, 109, 539
HphI GGTGA 2 cut(s) 361, 527
Hpy166II GTNNAC 2 cut(s) 97, 277
Hpy188I TCNGA 2 cut(s) 139, 560
Hpy188III TCNNGA 2 cut(s) 109, 539
Hpy8I GTNNAC 2 cut(s) 97, 277
Hpy99I CGWCG 6 cut(s) 221, 224, 278, 389, 392, 395
HpyCH4III ACNGT 3 cut(s) 233, 404, 416
HpyCH4IV ACGT 2 cut(s) 219, 273
HpyCH4V TGCA 1 cut(s) 521
HpyF10VI GCNNNNNNNGC 5 cut(s) 209, 329, 335, 338, 530
HpyF3I CTNAG 1 cut(s) 450
HpySE526I ACGT 2 cut(s) 219, 273
Hsp92I GRCGYC 3 cut(s) 105, 219, 273
Hsp92II CATG 2 cut(s) 489, 537
HspAI GCGC 1 cut(s) 154
Kpn2I TCCGGA 2 cut(s) 108, 538
Kzo9I GATC 1 cut(s) 394
Lsp1109I GCAGC 6 cut(s) 215, 335, 338, 341, 350, 533
LweI GCATC 1 cut(s) 508
MaeI CTAG 2 cut(s) 176, 309
MaeII ACGT 2 cut(s) 219, 273
MaeIII GTNAC 1 cut(s) 162
MalI GATC 1 cut(s) 396
MboI GATC 1 cut(s) 394
MboII GAAGA 1 cut(s) 35
MhlI GDGCHC 2 cut(s) 38, 195
MluCI AATT 2 cut(s) 141, 481
MlyI GAGTC 2 cut(s) 59, 357
MnlI CCTC 6 cut(s) 71, 95, 111, 262, 535, 541
MroI TCCGGA 2 cut(s) 108, 538
MslI CAYNNNNRTG 1 cut(s) 402
MspA1I CMGCKG 2 cut(s) 256, 524
MspI CCGG 3 cut(s) 92, 109, 539
MspR9I CCNGG 2 cut(s) 92, 289
MvaI CCWGG 1 cut(s) 289
MvnI CGCG 1 cut(s) 154
MwoI GCNNNNNNNGC 5 cut(s) 209, 329, 335, 338, 530
NciI CCSGG 1 cut(s) 92
NdeII GATC 1 cut(s) 394
NlaIII CATG 2 cut(s) 489, 537
NlaIV GGNNCC 2 cut(s) 82, 150
NmuCI GTSAC 1 cut(s) 162
NspI RCATGY 1 cut(s) 537
PceI AGGCCT 1 cut(s) 212
PcsI WCGNNNNNNNCGW 1 cut(s) 390
PkrI GCNGC 8 cut(s) 205, 226, 325, 328, 331, 340, 523, 526
PleI GAGTC 2 cut(s) 59, 356
PpsI GAGTC 2 cut(s) 59, 356
Psp6I CCWGG 1 cut(s) 287
PspGI CCWGG 1 cut(s) 287
PspN4I GGNNCC 2 cut(s) 82, 150
PspPI GGNCC 1 cut(s) 291
PvuII CAGCTG 1 cut(s) 256
RsaI GTAC 2 cut(s) 98, 304
RsaNI GTAC 2 cut(s) 97, 303
RseI CAYNNNNRTG 1 cut(s) 402
SalI GTCGAC 1 cut(s) 275
SatI GCNGC 8 cut(s) 204, 225, 324, 327, 330, 339, 522, 525
Sau3AI GATC 1 cut(s) 394
Sau96I GGNCC 1 cut(s) 291
SchI GAGTC 2 cut(s) 59, 357
ScrFI CCNGG 2 cut(s) 92, 289
SduI GDGCHC 2 cut(s) 38, 195
SetI ASST 7 cut(s) 76, 122, 181, 222, 258, 276, 517
SfaNI GCATC 1 cut(s) 508
SmiMI CAYNNNNRTG 1 cut(s) 402
Sse9I AATT 2 cut(s) 141, 481
SseBI AGGCCT 1 cut(s) 212
SsiI CCGC 8 cut(s) 85, 115, 152, 225, 294, 468, 524, 527
SspMI CTAG 2 cut(s) 176, 309
StuI AGGCCT 1 cut(s) 212
StyD4I CCNGG 2 cut(s) 90, 287
StyI CCWWGG 1 cut(s) 207
TaaI ACNGT 3 cut(s) 233, 404, 416
TaiI ACGT 2 cut(s) 222, 276
TaqI TCGA 1 cut(s) 276
TasI AATT 2 cut(s) 141, 481
TauI GCSGC 2 cut(s) 227, 527
TscAI CASTG 1 cut(s) 25
TseFI GTSAC 1 cut(s) 162
TseI GCWGC 6 cut(s) 203, 323, 326, 329, 338, 521
Tsp45I GTSAC 1 cut(s) 162
TspDTI ATGAA 2 cut(s) 473, 474
TspRI CASTG 1 cut(s) 25
XapI RAATTY 1 cut(s) 481
XceI RCATGY 1 cut(s) 537
XmiI GTMKAC 1 cut(s) 276
XspI CTAG 2 cut(s) 176, 309
ZraI GACGTC 2 cut(s) 220, 274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.