Rw1G025570

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0016007
Physical Location & Seq
Forward (+)
52334659 .. 52335996
1338 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G025570.1

Sequence Viewer

Length: 609 bp
ATGACGCTCCCAGCTAATGCTGCTTTCTTAACGTACATGGAGCAACCACCAGACACTGAAGATGACCTGAGCACTCCCACCACCACCACTGCCGCAGCCACTATTACCCAAAACCACGAAAACCAACAACGCAGCGGCACCCGGCACCCGGTGTACCGGGGCGTGAGGAAGCGGCGGTGGGGAAAATGGGTGTCCGAAATCAGGGAGCCACGCAAGAAGTCACGCATTTGGCTAGGGTCCTTCCCGGTGCCCGAGATGGCGGCCAAGGCCTATGACGTGGCGGCCTACTGCCTCAAGGGGCGAAAAGCGCAGCTGAACTTCCCCGACGAGGTGGAGCACTTGCCGAGGCCCTCCACGTGTACGCCTAGGGATATTCAGGCAGCAGCAGCTAAAGCAGCACAGACAATGAAGTCATCAAGTACAGCTAGAAGCACTGATCAGGATCATCACCGTGATGTATTGGACGGCGGCGGCGTCGATGATTTTTGGGGTGAGATTGAGCTACCGGAGCTAATGAATAGCGGCACGTGCTACTCGAACTCATGTGGGTGGAGTACGTTTACCGGTGAAACAGCATTAACATGGCCAGAAGGAGAGGCATGTCTATAA

Protein Analysis

202

Amino Acids

22.44

Weight (kDa)

5.97

Isoelectric Point (pI)

52.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 50 - 100 1.1e-13 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016007)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01250
fragaria_vesca FvH4_7g16810
malus_domestica MD01G1083000.v1.1 MD07G1151700.v1.1
prunus_persica Prupe.2G190200_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0360021
rosa_laevigata RLG00000027838
rosa_multiflora Rmu_ssc0000368.1_g000032
rosa_roxburghii Rroxscaffold_4G00296450
rosa_rugosa Rorug01G0274600
rosa_samantha Rh1AG288400 Rh1BG253900 Rh1CG271400 Rh1DG283000
rosa_wichuraiana Rw1G025570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 409
AccB1I GGYRCC 3 cut(s) 137, 144, 247
AciI CCGC 9 cut(s) 93, 135, 172, 175, 260, 281, 468, 471, 522
AclWI GGATC 1 cut(s) 450
AcoI YGGCCR 2 cut(s) 261, 584
AcuI CTGAAG 1 cut(s) 78
AcvI CACGTG 2 cut(s) 357, 528
AcyI GRCGYC 1 cut(s) 474
AdeI CACNNNGTG 1 cut(s) 151
AfaI GTAC 5 cut(s) 35, 155, 361, 421, 556
AfiI CCNNNNNNNGG 3 cut(s) 148, 201, 328
AflIII ACRYGT 1 cut(s) 356
AgeI ACCGGT 1 cut(s) 563
AjiI CACGTC 1 cut(s) 277
AluBI AGCT 6 cut(s) 14, 313, 389, 425, 502, 511
AluI AGCT 6 cut(s) 14, 313, 389, 425, 502, 511
Alw21I GWGCWC 2 cut(s) 74, 339
AlwI GGATC 1 cut(s) 450
AlwNI CAGNNNCTG 1 cut(s) 56
Ama87I CYCGRG 1 cut(s) 251
AoxI GGCC 5 cut(s) 261, 267, 282, 347, 584
ApeKI GCWGC 8 cut(s) 20, 95, 132, 310, 380, 383, 386, 395
AsiGI ACCGGT 1 cut(s) 563
AspA2I CCTAGG 1 cut(s) 365
AspLEI GCGC 1 cut(s) 310
AspS9I GGNCC 2 cut(s) 237, 348
AsuC2I CCSGG 4 cut(s) 142, 149, 158, 245
AsuHPI GGTGA 3 cut(s) 440, 503, 578
AvaI CYCGRG 1 cut(s) 251
AvaII GGWCC 1 cut(s) 237
AvrII CCTAGG 1 cut(s) 365
BaeGI GKGCMC 1 cut(s) 252
BalI TGGCCA 1 cut(s) 586
BanI GGYRCC 3 cut(s) 137, 144, 247
BbrPI CACGTG 2 cut(s) 357, 528
Bbv12I GWGCWC 2 cut(s) 74, 339
BbvI GCAGC 8 cut(s) 7, 107, 144, 322, 392, 395, 398, 407
BccI CCATC 1 cut(s) 250
BceAI ACGGC 1 cut(s) 481
BclI TGATCA 1 cut(s) 436
BcnI CCSGG 4 cut(s) 142, 149, 158, 245
BfaI CTAG 3 cut(s) 233, 366, 426
BlnI CCTAGG 1 cut(s) 365
Bme1390I CCNGG 4 cut(s) 142, 149, 158, 245
Bme18I GGWCC 1 cut(s) 237
BmeT110I CYCGRG 1 cut(s) 251
BmgBI CACGTC 1 cut(s) 277
BmgT120I GGNCC 2 cut(s) 237, 348
BmiI GGNNCC 5 cut(s) 139, 146, 207, 238, 249
BmrFI CCNGG 4 cut(s) 142, 149, 158, 245
Bpu10I CCTNAGC 1 cut(s) 68
BpuEI CTTGAG 1 cut(s) 278
BpuMI CCSGG 4 cut(s) 142, 149, 158, 245
BsaAI YACGTR 2 cut(s) 357, 528
BsaBI GATNNNNATC 1 cut(s) 441
BsaHI GRCGYC 1 cut(s) 474
BsaJI CCNNGG 4 cut(s) 157, 264, 344, 365
BsaWI WCCGGW 2 cut(s) 505, 563
Bsc4I CCNNNNNNNGG 3 cut(s) 148, 201, 328
Bse118I RCCGGY 1 cut(s) 563
Bse8I GATNNNNATC 1 cut(s) 441
BseDI CCNNGG 4 cut(s) 157, 264, 344, 365
BseJI GATNNNNATC 1 cut(s) 441
BseLI CCNNNNNNNGG 3 cut(s) 148, 201, 328
BseMII CTCAG 1 cut(s) 59
BseSI GKGCMC 1 cut(s) 252
BseXI GCAGC 8 cut(s) 7, 107, 144, 322, 392, 395, 398, 407
BseYI CCCAGC 1 cut(s) 10
BshFI GGCC 5 cut(s) 263, 269, 284, 349, 586
BshNI GGYRCC 3 cut(s) 137, 144, 247
BshTI ACCGGT 1 cut(s) 563
BsiHKAI GWGCWC 2 cut(s) 74, 339
BsiHKCI CYCGRG 1 cut(s) 251
BsiSI CCGG 6 cut(s) 142, 149, 157, 245, 506, 564
BslI CCNNNNNNNGG 3 cut(s) 148, 201, 328
BsnI GGCC 5 cut(s) 263, 269, 284, 349, 586
BsoBI CYCGRG 1 cut(s) 251
Bsp1286I GDGCHC 3 cut(s) 74, 252, 339
Bsp143I GATC 2 cut(s) 436, 442
BspACI CCGC 9 cut(s) 93, 135, 172, 175, 260, 281, 468, 471, 522
BspANI GGCC 5 cut(s) 263, 269, 284, 349, 586
BspCNI CTCAG 1 cut(s) 60
BspLI GGNNCC 5 cut(s) 139, 146, 207, 238, 249
BspPI GGATC 1 cut(s) 450
BspT107I GGYRCC 3 cut(s) 137, 144, 247
BsrFI RCCGGY 1 cut(s) 563
BssAI RCCGGY 1 cut(s) 563
BssECI CCNNGG 4 cut(s) 157, 264, 344, 365
BssMI GATC 2 cut(s) 436, 442
BssNI GRCGYC 1 cut(s) 474
BssT1I CCWWGG 2 cut(s) 264, 365
Bst4CI ACNGT 1 cut(s) 452
BstACI GRCGYC 1 cut(s) 474
BstBAI YACGTR 2 cut(s) 357, 528
BstDEI CTNAG 1 cut(s) 68
BstHHI GCGC 1 cut(s) 310
BstKTI GATC 2 cut(s) 439, 445
BstMBI GATC 2 cut(s) 436, 442
BstMWI GCNNNNNNNGC 8 cut(s) 20, 266, 307, 386, 392, 395, 508, 528
BstNSI RCATGY 1 cut(s) 603
BstSCI CCNGG 4 cut(s) 140, 147, 156, 243
BstSLI GKGCMC 1 cut(s) 252
BstV1I GCAGC 8 cut(s) 7, 107, 144, 322, 392, 395, 398, 407
BsuRI GGCC 5 cut(s) 263, 269, 284, 349, 586
BtrI CACGTC 1 cut(s) 277
BtsI GCAGTG 1 cut(s) 87
BtsIMutI CAGTG 3 cut(s) 54, 87, 432
CaiI CAGNNNCTG 1 cut(s) 56
CfoI GCGC 1 cut(s) 310
Cfr10I RCCGGY 1 cut(s) 563
Cfr13I GGNCC 2 cut(s) 237, 348
CseI GACGC 2 cut(s) 13, 463
Csp6I GTAC 5 cut(s) 34, 154, 360, 420, 555
CspAI ACCGGT 1 cut(s) 563
CviAII CATG 4 cut(s) 37, 543, 582, 600
CviQI GTAC 5 cut(s) 34, 154, 360, 420, 555
DdeI CTNAG 1 cut(s) 68
DpnI GATC 2 cut(s) 438, 444
DpnII GATC 2 cut(s) 436, 442
DraIII CACNNNGTG 1 cut(s) 151
DrdI GACNNNNNNGTC 1 cut(s) 409
DseDI GACNNNNNNGTC 1 cut(s) 409
EaeI YGGCCR 2 cut(s) 261, 584
Eco130I CCWWGG 2 cut(s) 264, 365
Eco147I AGGCCT 1 cut(s) 269
Eco47I GGWCC 1 cut(s) 237
Eco57I CTGAAG 1 cut(s) 78
Eco72I CACGTG 2 cut(s) 357, 528
Eco88I CYCGRG 1 cut(s) 251
EcoO109I RGGNCCY 2 cut(s) 237, 348
EcoT14I CCWWGG 2 cut(s) 264, 365
ErhI CCWWGG 2 cut(s) 264, 365
FaeI CATG 4 cut(s) 40, 546, 585, 603
FaiI YATR 6 cut(s) 38, 273, 544, 583, 601, 607
FatI CATG 4 cut(s) 36, 542, 581, 599
FbaI TGATCA 1 cut(s) 436
FspBI CTAG 3 cut(s) 233, 366, 426
GlaI GCGC 1 cut(s) 309
GsaI CCCAGC 1 cut(s) 14
HaeIII GGCC 5 cut(s) 263, 269, 284, 349, 586
HapII CCGG 6 cut(s) 142, 149, 157, 245, 506, 564
HgaI GACGC 2 cut(s) 13, 463
HhaI GCGC 1 cut(s) 310
Hin1I GRCGYC 1 cut(s) 474
Hin1II CATG 4 cut(s) 40, 546, 585, 603
Hin6I GCGC 1 cut(s) 308
HinP1I GCGC 1 cut(s) 308
HpaII CCGG 6 cut(s) 142, 149, 157, 245, 506, 564
HphI GGTGA 3 cut(s) 440, 503, 578
Hpy166II GTNNAC 3 cut(s) 154, 360, 561
Hpy188I TCNGA 1 cut(s) 196
Hpy188III TCNNGA 1 cut(s) 440
Hpy8I GTNNAC 3 cut(s) 154, 360, 561
Hpy99I CGWCG 2 cut(s) 329, 479
HpyAV CCTTC 2 cut(s) 250, 584
HpyCH4III ACNGT 1 cut(s) 452
HpyCH4IV ACGT 5 cut(s) 32, 276, 356, 527, 557
HpyF10VI GCNNNNNNNGC 8 cut(s) 20, 266, 307, 386, 392, 395, 508, 528
HpyF3I CTNAG 1 cut(s) 68
HpySE526I ACGT 5 cut(s) 32, 276, 356, 527, 557
Hsp92I GRCGYC 1 cut(s) 474
Hsp92II CATG 4 cut(s) 40, 546, 585, 603
HspAI GCGC 1 cut(s) 308
Ksp22I TGATCA 1 cut(s) 436
Kzo9I GATC 2 cut(s) 436, 442
LmnI GCTCC 5 cut(s) 12, 40, 205, 334, 508
Lsp1109I GCAGC 8 cut(s) 7, 107, 144, 322, 392, 395, 398, 407
MaeI CTAG 3 cut(s) 233, 366, 426
MaeII ACGT 5 cut(s) 32, 276, 356, 527, 557
MaeIII GTNAC 1 cut(s) 219
MalI GATC 2 cut(s) 438, 444
MboI GATC 2 cut(s) 436, 442
MboII GAAGA 1 cut(s) 71
MhlI GDGCHC 3 cut(s) 74, 252, 339
MlsI TGGCCA 1 cut(s) 586
MluNI TGGCCA 1 cut(s) 586
MnlI CCTC 6 cut(s) 159, 302, 322, 339, 361, 589
Mox20I TGGCCA 1 cut(s) 586
MscI TGGCCA 1 cut(s) 586
MseI TTAA 2 cut(s) 29, 578
MslI CAYNNNNRTG 4 cut(s) 450, 453, 547, 580
Msp20I TGGCCA 1 cut(s) 586
MspA1I CMGCKG 2 cut(s) 135, 313
MspI CCGG 6 cut(s) 142, 149, 157, 245, 506, 564
MspR9I CCNGG 4 cut(s) 142, 149, 158, 245
MwoI GCNNNNNNNGC 8 cut(s) 20, 266, 307, 386, 392, 395, 508, 528
NciI CCSGG 4 cut(s) 142, 149, 158, 245
NdeII GATC 2 cut(s) 436, 442
NlaIII CATG 4 cut(s) 40, 546, 585, 603
NlaIV GGNNCC 5 cut(s) 139, 146, 207, 238, 249
NmeAIII GCCGAG 1 cut(s) 369
NmuCI GTSAC 1 cut(s) 219
NspI RCATGY 1 cut(s) 603
PceI AGGCCT 1 cut(s) 269
PcsI WCGNNNNNNNCGW 2 cut(s) 471, 533
PinAI ACCGGT 1 cut(s) 563
PmaCI CACGTG 2 cut(s) 357, 528
PmlI CACGTG 2 cut(s) 357, 528
Ppu21I YACGTR 2 cut(s) 357, 528
PpuMI RGGWCCY 1 cut(s) 237
Psp5II RGGWCCY 1 cut(s) 237
PspCI CACGTG 2 cut(s) 357, 528
PspFI CCCAGC 1 cut(s) 10
PspN4I GGNNCC 5 cut(s) 139, 146, 207, 238, 249
PspPI GGNCC 2 cut(s) 237, 348
PspPPI RGGWCCY 1 cut(s) 237
PstNI CAGNNNCTG 1 cut(s) 56
PvuII CAGCTG 1 cut(s) 313
RsaI GTAC 5 cut(s) 35, 155, 361, 421, 556
RsaNI GTAC 5 cut(s) 34, 154, 360, 420, 555
RseI CAYNNNNRTG 4 cut(s) 450, 453, 547, 580
SaqAI TTAA 2 cut(s) 29, 578
Sau3AI GATC 2 cut(s) 436, 442
Sau96I GGNCC 2 cut(s) 237, 348
ScrFI CCNGG 4 cut(s) 142, 149, 158, 245
SduI GDGCHC 3 cut(s) 74, 252, 339
SinI GGWCC 1 cut(s) 237
SmiMI CAYNNNNRTG 4 cut(s) 450, 453, 547, 580
SmlI CTYRAG 1 cut(s) 293
SmoI CTYRAG 1 cut(s) 293
SseBI AGGCCT 1 cut(s) 269
SsiI CCGC 9 cut(s) 93, 135, 172, 175, 260, 281, 468, 471, 522
SspMI CTAG 3 cut(s) 233, 366, 426
StuI AGGCCT 1 cut(s) 269
StyD4I CCNGG 4 cut(s) 140, 147, 156, 243
StyI CCWWGG 2 cut(s) 264, 365
TaaI ACNGT 1 cut(s) 452
TaiI ACGT 5 cut(s) 35, 279, 359, 530, 560
TaqI TCGA 2 cut(s) 477, 536
TatI WGTACW 1 cut(s) 419
TauI GCSGC 8 cut(s) 95, 138, 175, 263, 284, 471, 474, 525
Tru1I TTAA 2 cut(s) 29, 578
Tru9I TTAA 2 cut(s) 29, 578
TscAI CASTG 3 cut(s) 61, 94, 439
TseFI GTSAC 1 cut(s) 219
TseI GCWGC 8 cut(s) 20, 95, 132, 310, 380, 383, 386, 395
Tsp45I GTSAC 1 cut(s) 219
TspDTI ATGAA 2 cut(s) 422, 530
TspRI CASTG 3 cut(s) 61, 94, 439
VpaK11BI GGWCC 1 cut(s) 237
XceI RCATGY 1 cut(s) 603
XmaJI CCTAGG 1 cut(s) 365
XspI CTAG 3 cut(s) 233, 366, 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.