RchiOBHm_Chr1g0360021

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0016007
Physical Location & Seq
Forward (+)
51851839 .. 51852621
783 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ58504

Sequence Viewer

Length: 636 bp
ATGACACTCCCAGCTAATGCTTTCTCAACGTACATGGAGCAACCACCAGACACTGAAGATGACCTGAGCACTCCCACCACCACCACTGCCGCAGCCACAATCACCCAGAACCACGAAAACCAACAACGCAGCGGCACCCGGCACCCGGTGTACCGGGGCGTGAGGAAGCGGAGGTGGGGAAAATGGGTGTCCGAAATCAGGGAGCCACGCAAGAAGTCACGCATTTGGCTAGGGTCCTTCCCGGTGCCCGAGATGGCGGCCAAGGCCTATGACGTGGCGGCCTACTGCCTCAAGGGGCGAAAAGCGCAGCTGAACTTCCCCGACGAGGTGGAGCACTTGCCGAGGCCCTCCACGAGTACGGCTAGGGATATTCAGGCAGCAGCAGCTAAAGCAGCACAGACAATGAAGTCATCAAGTACAGCTAGAAGCACTGATCAGGATCATCACCGTGACGTATTGGACGGCGGCGGCGTCGATGATTTTTGGGGTGAGATTGAGCTACCGGAGCTAATGAATAGCGGCACGTGCTACTCGAACTCATGTGGGTGGAGTACGTTTACCGGTGAAACGGTGAAACGGAGTACGTTTACCGGTGAAACAGCATTAACATGGCCAGAAGGAGAGGCATGTCTATAA

Protein Analysis

211

Amino Acids

23.4

Weight (kDa)

6.22

Isoelectric Point (pI)

52.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 49 - 99 1.2e-13 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016007)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01250
fragaria_vesca FvH4_7g16810
malus_domestica MD01G1083000.v1.1 MD07G1151700.v1.1
prunus_persica Prupe.2G190200_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0360021
rosa_laevigata RLG00000027838
rosa_multiflora Rmu_ssc0000368.1_g000032
rosa_roxburghii Rroxscaffold_4G00296450
rosa_rugosa Rorug01G0274600
rosa_samantha Rh1AG288400 Rh1BG253900 Rh1CG271400 Rh1DG283000
rosa_wichuraiana Rw1G025570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 406
AccB1I GGYRCC 3 cut(s) 134, 141, 244
AciI CCGC 8 cut(s) 90, 132, 169, 257, 278, 465, 468, 519
AclWI GGATC 1 cut(s) 447
AcoI YGGCCR 2 cut(s) 258, 611
AcuI CTGAAG 1 cut(s) 75
AcvI CACGTG 1 cut(s) 525
AcyI GRCGYC 1 cut(s) 471
AdeI CACNNNGTG 1 cut(s) 148
AfaI GTAC 6 cut(s) 32, 152, 358, 418, 553, 583
AfiI CCNNNNNNNGG 3 cut(s) 145, 198, 325
AgeI ACCGGT 2 cut(s) 560, 590
AjiI CACGTC 1 cut(s) 274
AluBI AGCT 6 cut(s) 14, 310, 386, 422, 499, 508
AluI AGCT 6 cut(s) 14, 310, 386, 422, 499, 508
Alw21I GWGCWC 2 cut(s) 71, 336
AlwI GGATC 1 cut(s) 447
AlwNI CAGNNNCTG 1 cut(s) 53
Ama87I CYCGRG 1 cut(s) 248
AoxI GGCC 5 cut(s) 258, 264, 279, 344, 611
ApeKI GCWGC 7 cut(s) 92, 129, 307, 377, 380, 383, 392
AsiGI ACCGGT 2 cut(s) 560, 590
AspLEI GCGC 1 cut(s) 307
AspS9I GGNCC 2 cut(s) 234, 345
AsuC2I CCSGG 4 cut(s) 139, 146, 155, 242
AsuHPI GGTGA 6 cut(s) 94, 437, 500, 575, 583, 605
AvaI CYCGRG 1 cut(s) 248
AvaII GGWCC 1 cut(s) 234
BaeGI GKGCMC 1 cut(s) 249
BalI TGGCCA 1 cut(s) 613
BanI GGYRCC 3 cut(s) 134, 141, 244
BauI CACGAG 1 cut(s) 352
BbrPI CACGTG 1 cut(s) 525
Bbv12I GWGCWC 2 cut(s) 71, 336
BbvI GCAGC 7 cut(s) 104, 141, 319, 389, 392, 395, 404
BccI CCATC 1 cut(s) 247
BceAI ACGGC 2 cut(s) 375, 478
BclI TGATCA 1 cut(s) 433
BcnI CCSGG 4 cut(s) 139, 146, 155, 242
BfaI CTAG 3 cut(s) 230, 363, 423
Bme1390I CCNGG 4 cut(s) 139, 146, 155, 242
Bme18I GGWCC 1 cut(s) 234
BmeT110I CYCGRG 1 cut(s) 248
BmgBI CACGTC 1 cut(s) 274
BmgT120I GGNCC 2 cut(s) 234, 345
BmiI GGNNCC 5 cut(s) 136, 143, 204, 235, 246
BmrFI CCNGG 4 cut(s) 139, 146, 155, 242
Bpu10I CCTNAGC 1 cut(s) 65
BpuEI CTTGAG 1 cut(s) 275
BpuMI CCSGG 4 cut(s) 139, 146, 155, 242
BsaAI YACGTR 1 cut(s) 525
BsaBI GATNNNNATC 1 cut(s) 438
BsaHI GRCGYC 1 cut(s) 471
BsaJI CCNNGG 3 cut(s) 154, 261, 341
BsaWI WCCGGW 3 cut(s) 502, 560, 590
Bsc4I CCNNNNNNNGG 3 cut(s) 145, 198, 325
Bse118I RCCGGY 2 cut(s) 560, 590
Bse8I GATNNNNATC 1 cut(s) 438
BseDI CCNNGG 3 cut(s) 154, 261, 341
BseJI GATNNNNATC 1 cut(s) 438
BseLI CCNNNNNNNGG 3 cut(s) 145, 198, 325
BseMII CTCAG 1 cut(s) 56
BseSI GKGCMC 1 cut(s) 249
BseXI GCAGC 7 cut(s) 104, 141, 319, 389, 392, 395, 404
BseYI CCCAGC 1 cut(s) 10
BshFI GGCC 5 cut(s) 260, 266, 281, 346, 613
BshNI GGYRCC 3 cut(s) 134, 141, 244
BshTI ACCGGT 2 cut(s) 560, 590
BsiHKAI GWGCWC 2 cut(s) 71, 336
BsiHKCI CYCGRG 1 cut(s) 248
BsiSI CCGG 7 cut(s) 139, 146, 154, 242, 503, 561, 591
BslI CCNNNNNNNGG 3 cut(s) 145, 198, 325
BsnI GGCC 5 cut(s) 260, 266, 281, 346, 613
BsoBI CYCGRG 1 cut(s) 248
Bsp1286I GDGCHC 3 cut(s) 71, 249, 336
Bsp143I GATC 2 cut(s) 433, 439
BspACI CCGC 8 cut(s) 90, 132, 169, 257, 278, 465, 468, 519
BspANI GGCC 5 cut(s) 260, 266, 281, 346, 613
BspCNI CTCAG 1 cut(s) 57
BspLI GGNNCC 5 cut(s) 136, 143, 204, 235, 246
BspPI GGATC 1 cut(s) 447
BspT107I GGYRCC 3 cut(s) 134, 141, 244
BsrFI RCCGGY 2 cut(s) 560, 590
BssAI RCCGGY 2 cut(s) 560, 590
BssECI CCNNGG 3 cut(s) 154, 261, 341
BssMI GATC 2 cut(s) 433, 439
BssNI GRCGYC 1 cut(s) 471
BssSI CACGAG 1 cut(s) 352
BssT1I CCWWGG 1 cut(s) 261
Bst2BI CACGAG 1 cut(s) 352
Bst4CI ACNGT 2 cut(s) 449, 571
BstACI GRCGYC 1 cut(s) 471
BstBAI YACGTR 1 cut(s) 525
BstDEI CTNAG 1 cut(s) 65
BstHHI GCGC 1 cut(s) 307
BstKTI GATC 2 cut(s) 436, 442
BstMBI GATC 2 cut(s) 433, 439
BstMWI GCNNNNNNNGC 7 cut(s) 263, 304, 383, 389, 392, 505, 525
BstNSI RCATGY 1 cut(s) 630
BstSCI CCNGG 4 cut(s) 137, 144, 153, 240
BstSLI GKGCMC 1 cut(s) 249
BstV1I GCAGC 7 cut(s) 104, 141, 319, 389, 392, 395, 404
BsuRI GGCC 5 cut(s) 260, 266, 281, 346, 613
BtrI CACGTC 1 cut(s) 274
BtsI GCAGTG 1 cut(s) 84
BtsIMutI CAGTG 3 cut(s) 51, 84, 429
CaiI CAGNNNCTG 1 cut(s) 53
CfoI GCGC 1 cut(s) 307
Cfr10I RCCGGY 2 cut(s) 560, 590
Cfr13I GGNCC 2 cut(s) 234, 345
CseI GACGC 1 cut(s) 460
Csp6I GTAC 6 cut(s) 31, 151, 357, 417, 552, 582
CspAI ACCGGT 2 cut(s) 560, 590
CviAII CATG 4 cut(s) 34, 540, 609, 627
CviQI GTAC 6 cut(s) 31, 151, 357, 417, 552, 582
DdeI CTNAG 1 cut(s) 65
DpnI GATC 2 cut(s) 435, 441
DpnII GATC 2 cut(s) 433, 439
DraIII CACNNNGTG 1 cut(s) 148
DrdI GACNNNNNNGTC 1 cut(s) 406
DseDI GACNNNNNNGTC 1 cut(s) 406
EaeI YGGCCR 2 cut(s) 258, 611
Eco130I CCWWGG 1 cut(s) 261
Eco147I AGGCCT 1 cut(s) 266
Eco47I GGWCC 1 cut(s) 234
Eco57I CTGAAG 1 cut(s) 75
Eco72I CACGTG 1 cut(s) 525
Eco88I CYCGRG 1 cut(s) 248
EcoO109I RGGNCCY 2 cut(s) 234, 345
EcoT14I CCWWGG 1 cut(s) 261
ErhI CCWWGG 1 cut(s) 261
FaeI CATG 4 cut(s) 37, 543, 612, 630
FaiI YATR 6 cut(s) 35, 270, 541, 610, 628, 634
FatI CATG 4 cut(s) 33, 539, 608, 626
FbaI TGATCA 1 cut(s) 433
FspBI CTAG 3 cut(s) 230, 363, 423
GlaI GCGC 1 cut(s) 306
GsaI CCCAGC 1 cut(s) 14
HaeIII GGCC 5 cut(s) 260, 266, 281, 346, 613
HapII CCGG 7 cut(s) 139, 146, 154, 242, 503, 561, 591
HgaI GACGC 1 cut(s) 460
HhaI GCGC 1 cut(s) 307
Hin1I GRCGYC 1 cut(s) 471
Hin1II CATG 4 cut(s) 37, 543, 612, 630
Hin6I GCGC 1 cut(s) 305
HinP1I GCGC 1 cut(s) 305
HpaII CCGG 7 cut(s) 139, 146, 154, 242, 503, 561, 591
HphI GGTGA 6 cut(s) 94, 437, 500, 575, 583, 605
Hpy166II GTNNAC 3 cut(s) 151, 558, 588
Hpy188I TCNGA 1 cut(s) 193
Hpy188III TCNNGA 1 cut(s) 437
Hpy8I GTNNAC 3 cut(s) 151, 558, 588
Hpy99I CGWCG 2 cut(s) 326, 476
HpyAV CCTTC 2 cut(s) 247, 611
HpyCH4III ACNGT 2 cut(s) 449, 571
HpyCH4IV ACGT 6 cut(s) 29, 273, 453, 524, 554, 584
HpyF10VI GCNNNNNNNGC 7 cut(s) 263, 304, 383, 389, 392, 505, 525
HpyF3I CTNAG 1 cut(s) 65
HpySE526I ACGT 6 cut(s) 29, 273, 453, 524, 554, 584
Hsp92I GRCGYC 1 cut(s) 471
Hsp92II CATG 4 cut(s) 37, 543, 612, 630
HspAI GCGC 1 cut(s) 305
Ksp22I TGATCA 1 cut(s) 433
Kzo9I GATC 2 cut(s) 433, 439
LmnI GCTCC 4 cut(s) 37, 202, 331, 505
Lsp1109I GCAGC 7 cut(s) 104, 141, 319, 389, 392, 395, 404
MaeI CTAG 3 cut(s) 230, 363, 423
MaeII ACGT 6 cut(s) 29, 273, 453, 524, 554, 584
MaeIII GTNAC 2 cut(s) 216, 449
MalI GATC 2 cut(s) 435, 441
MboI GATC 2 cut(s) 433, 439
MboII GAAGA 1 cut(s) 68
MhlI GDGCHC 3 cut(s) 71, 249, 336
MlsI TGGCCA 1 cut(s) 613
MluNI TGGCCA 1 cut(s) 613
MnlI CCTC 7 cut(s) 156, 165, 299, 319, 336, 358, 616
Mox20I TGGCCA 1 cut(s) 613
MscI TGGCCA 1 cut(s) 613
MseI TTAA 1 cut(s) 605
MslI CAYNNNNRTG 3 cut(s) 447, 544, 607
Msp20I TGGCCA 1 cut(s) 613
MspA1I CMGCKG 2 cut(s) 132, 310
MspI CCGG 7 cut(s) 139, 146, 154, 242, 503, 561, 591
MspR9I CCNGG 4 cut(s) 139, 146, 155, 242
MwoI GCNNNNNNNGC 7 cut(s) 263, 304, 383, 389, 392, 505, 525
NciI CCSGG 4 cut(s) 139, 146, 155, 242
NdeII GATC 2 cut(s) 433, 439
NlaIII CATG 4 cut(s) 37, 543, 612, 630
NlaIV GGNNCC 5 cut(s) 136, 143, 204, 235, 246
NmeAIII GCCGAG 1 cut(s) 366
NmuCI GTSAC 2 cut(s) 216, 449
NspI RCATGY 1 cut(s) 630
PceI AGGCCT 1 cut(s) 266
PcsI WCGNNNNNNNCGW 2 cut(s) 468, 530
PinAI ACCGGT 2 cut(s) 560, 590
PmaCI CACGTG 1 cut(s) 525
PmlI CACGTG 1 cut(s) 525
Ppu21I YACGTR 1 cut(s) 525
PpuMI RGGWCCY 1 cut(s) 234
Psp5II RGGWCCY 1 cut(s) 234
PspCI CACGTG 1 cut(s) 525
PspFI CCCAGC 1 cut(s) 10
PspN4I GGNNCC 5 cut(s) 136, 143, 204, 235, 246
PspPI GGNCC 2 cut(s) 234, 345
PspPPI RGGWCCY 1 cut(s) 234
PstNI CAGNNNCTG 1 cut(s) 53
PvuII CAGCTG 1 cut(s) 310
RsaI GTAC 6 cut(s) 32, 152, 358, 418, 553, 583
RsaNI GTAC 6 cut(s) 31, 151, 357, 417, 552, 582
RseI CAYNNNNRTG 3 cut(s) 447, 544, 607
SaqAI TTAA 1 cut(s) 605
Sau3AI GATC 2 cut(s) 433, 439
Sau96I GGNCC 2 cut(s) 234, 345
ScrFI CCNGG 4 cut(s) 139, 146, 155, 242
SduI GDGCHC 3 cut(s) 71, 249, 336
SinI GGWCC 1 cut(s) 234
SmiMI CAYNNNNRTG 3 cut(s) 447, 544, 607
SmlI CTYRAG 1 cut(s) 290
SmoI CTYRAG 1 cut(s) 290
SseBI AGGCCT 1 cut(s) 266
SsiI CCGC 8 cut(s) 90, 132, 169, 257, 278, 465, 468, 519
SspMI CTAG 3 cut(s) 230, 363, 423
StuI AGGCCT 1 cut(s) 266
StyD4I CCNGG 4 cut(s) 137, 144, 153, 240
StyI CCWWGG 1 cut(s) 261
TaaI ACNGT 2 cut(s) 449, 571
TaiI ACGT 6 cut(s) 32, 276, 456, 527, 557, 587
TaqI TCGA 2 cut(s) 474, 533
TatI WGTACW 1 cut(s) 416
TauI GCSGC 7 cut(s) 92, 135, 260, 281, 468, 471, 522
Tru1I TTAA 1 cut(s) 605
Tru9I TTAA 1 cut(s) 605
TscAI CASTG 3 cut(s) 58, 91, 436
TseFI GTSAC 2 cut(s) 216, 449
TseI GCWGC 7 cut(s) 92, 129, 307, 377, 380, 383, 392
Tsp45I GTSAC 2 cut(s) 216, 449
TspDTI ATGAA 2 cut(s) 419, 527
TspGWI ACGGA 1 cut(s) 592
TspRI CASTG 3 cut(s) 58, 91, 436
VpaK11BI GGWCC 1 cut(s) 234
XceI RCATGY 1 cut(s) 630
XspI CTAG 3 cut(s) 230, 363, 423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.