FvH4_7g16940

Alkaline and neutral invertase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0008212
Physical Location & Seq
Forward (+)
14522025 .. 14525609
3585 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g16940.t1

Sequence Viewer

Length: 1752 bp
ATGTCGTCACCGCAGCTCGGGAAACTCAACGAGGAAAACGAACCAGAGGAGGAGGCTTCTCAGCCTCTGAATCATGCTACTGCTCCGGCGACGGCCTCGGAGACGCCGGAGATAGAGTCGCCGATGAGGCTTTCGGTTTCTGAGATGAAGAACAAGAAGAACAAAGAGAGGAAGAAGAAGAAGAAGAAGGCGGCGGCGAGAGATGCTGCAGCTGCGACGACTCATAGCGTTGAGTCCCAAATCTCTGAGAAAACGACGTCGTCGCTGCTCACGTTGCAGAGTATGGCGGTATCTACGTTACGTGAATTCTTGGTAGAATCTTTGGAGGAATCTCCGGCGACTATGGTGGAGGAGGCCTGGTATGGACTCAGGGAATCAATCGTTATCTATGAAGGCAAGCCGGTTGGCACTCTTGCTGCTATGGATTCAACGAAGGAGCCGCTGAACTACAATCAGGTGTTTGTGAGAGATTTTATTCCTGCTGGTTTGGCCTTCCTCATGAAGAAACCAGCAGAGCCAGAGATAGTAAAGAATTTTCTCCTAAAGACACTCCACCTCCAAGGTCGGGAGAAAAAGGTTGATAACTTTACACTCGGAGAAGGTGTACTTCCTGCGAGCTTTAAGGTTTCAACTGATTCCCCCGATGGCAAGGAAACCATCGTTGCAGATTTCGGTGGTAGCGCAATTGGAAGAGTTGCTCCTGTCGATTCTGGATTCTGGTGGATCATATTGCTGAGGGCATATACCAAATGCACTGGTGATCATACGCTGCGGGAGCGCCCTGAGGTGCAAAAGGGAATGAGACTAATTCTCAAACTCTGCCTTTCAGATGACCTTGACACATTCCCAACTCTTCTTTGTGCAGATGGCTGCAGTATGATTGACAGGAGGATGGGCATATATGGTTATCCAATAGAGATCCAAGCACTTTTCTACTTTGCATTGAGGTGTGCAGTGCAGCTGTTGAAGCCCGAGGATGGTGTTAAGGAGCTTCTTGAGAGGATAGCTAAGCGCATCGCAGCTCTCTGCTTTCACATCCAGCAGTACTACTGGCTTGATTTTAAGCAGCTCAATAACGTATACCGATACAAAACGGAGGAGTATTCCCATACTGCTGTCAACACGTTCAATGTGATGCCAGAGTCCATCCCTGAATGGGTTTTTGATTTCATGCCCAGCTCACGAGGAGGGTATTTGATTGGCAATGTTAGCCCAGCTCGCATGGACTTCCGGTGGTTCTTAGTTGGAAACTGCATTGCAATTTTGAGCGGTTTAGCCACAGATAAGCAAGCTACAGCCATCATGGATCTGATTGAGGAGCGTTGGGATGATTTGATTGGTGAGATGCCTCTGAAAATTGCATATCCAGCATTAGAGGGAGATGAGTGGAGGACAATCACAGGATGTGACTCCAAGAATACGCGATGGAGCTACCACAATGGCGGTTCATGGCCAACACTGTTGTGGCTGCTGGCTTCTGCTTGCAGCAAGACTGGGAGGCTACAGATTGCAAAGAGGGCAATAGAGCTGGCTGAGCAGCGGCTCTCCAAAGACGGGTGGCCTGAGTACTATGATGGTAAGACGGGGAAATACATTGGGAAGCAAGCAAGGAAGTTCCAGACATGGAGCATGTCTGGCTATCTGATTGCTAAGATGATGATAGAGAATCCAAACAATGTCTCATTGCTATCAATCGAAGGAGGACTCTCCCGGTCTACTTCAGATAACCACAGTGGCATACGCTACAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_100

Protein Analysis

584

Amino Acids

65.56

Weight (kDa)

6.52

Isoelectric Point (pI)

51.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_100 PF12899 119 - 557 8.3e-209 Alkaline and neutral invertase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 260
AccBSI CCGCTC 1 cut(s) 1269
AccI GTMKAC 2 cut(s) 1080, 1715
AccII CGCG 1 cut(s) 1423
AciI CCGC 9 cut(s) 11, 191, 194, 287, 440, 772, 1269, 1443, 1540
AclWI GGATC 3 cut(s) 731, 913, 1314
AcoI YGGCCR 1 cut(s) 1451
AcsI RAATTY 2 cut(s) 305, 532
AcuI CTGAAG 1 cut(s) 1704
AcyI GRCGYC 2 cut(s) 104, 257
AfaI GTAC 3 cut(s) 606, 1046, 1568
AfiI CCNNNNNNNGG 5 cut(s) 17, 565, 977, 1156, 1554
AflIII ACRYGT 1 cut(s) 1122
AgsI TTSAA 4 cut(s) 429, 630, 967, 1129
AjnI CCWGG 1 cut(s) 356
AleI CACNNNNGTG 1 cut(s) 1462
Alw26I GTCTC 3 cut(s) 95, 796, 1684
AlwI GGATC 3 cut(s) 731, 913, 1314
AlwNI CAGNNNCTG 1 cut(s) 67
Ama87I CYCGRG 2 cut(s) 17, 971
AoxI GGCC 5 cut(s) 93, 354, 489, 1451, 1559
ApoI RAATTY 2 cut(s) 305, 532
AspLEI GCGC 3 cut(s) 683, 780, 1014
AsuC2I CCSGG 1 cut(s) 1711
AsuHPI GGTGA 2 cut(s) 770, 1352
AvaI CYCGRG 2 cut(s) 17, 971
AxyI CCTNAGG 1 cut(s) 783
BalI TGGCCA 1 cut(s) 1453
BauI CACGAG 1 cut(s) 1182
BbvCI CCTCAGC 1 cut(s) 734
BccI CCATC 9 cut(s) 638, 665, 860, 886, 971, 1154, 1307, 1419, 1568
BceAI ACGGC 1 cut(s) 108
BciT130I CCWGG 1 cut(s) 358
BclI TGATCA 1 cut(s) 760
BcnI CCSGG 1 cut(s) 1711
BcoDI GTCTC 3 cut(s) 95, 796, 1684
BfmI CTRYAG 5 cut(s) 207, 871, 1293, 1502, 1744
BfoI RGCGCY 1 cut(s) 781
BglI GCCNNNNNGGC 1 cut(s) 127
BlpI GCTNAGC 2 cut(s) 1008, 1533
BmcAI AGTACT 2 cut(s) 1046, 1568
Bme1390I CCNGG 2 cut(s) 358, 1711
BmeT110I CYCGRG 2 cut(s) 17, 971
BmiI GGNNCC 1 cut(s) 438
BmrFI CCNGG 2 cut(s) 358, 1711
BmrI ACTGGG 1 cut(s) 1503
BmsI GCATC 4 cut(s) 193, 1023, 1125, 1335
BmuI ACTGGG 1 cut(s) 1503
Bpu10I CCTNAGC 1 cut(s) 734
Bpu1102I GCTNAGC 2 cut(s) 1008, 1533
BpuEI CTTGAG 1 cut(s) 1016
BpuMI CCSGG 1 cut(s) 1711
BsaAI YACGTR 1 cut(s) 302
BsaHI GRCGYC 2 cut(s) 104, 257
BsaJI CCNNGG 3 cut(s) 96, 559, 972
BsaWI WCCGGW 1 cut(s) 1230
BsaXI ACNNNNNCTCC 8 cut(s) 101, 131, 344, 374, 540, 570, 588, 618
Bsc4I CCNNNNNNNGG 5 cut(s) 17, 565, 977, 1156, 1554
Bse118I RCCGGY 1 cut(s) 400
Bse1I ACTGG 3 cut(s) 760, 1055, 1498
Bse21I CCTNAGG 1 cut(s) 783
Bse3DI GCAATG 3 cut(s) 1210, 1254, 1682
BseBI CCWGG 1 cut(s) 358
BseDI CCNNGG 3 cut(s) 96, 559, 972
BseGI GGATG 6 cut(s) 897, 982, 1035, 1146, 1333, 1409
BseLI CCNNNNNNNGG 5 cut(s) 17, 565, 977, 1156, 1554
BseMI GCAATG 3 cut(s) 1210, 1254, 1682
BseMII CTCAG 8 cut(s) 74, 132, 237, 382, 725, 774, 1524, 1554
BseNI ACTGG 3 cut(s) 760, 1055, 1498
BseRI GAGGAG 6 cut(s) 62, 65, 365, 1112, 1200, 1331
BseYI CCCAGC 2 cut(s) 1175, 1213
BsgI GTGCAG 3 cut(s) 882, 972, 977
Bsh1236I CGCG 1 cut(s) 1423
BshFI GGCC 5 cut(s) 95, 356, 491, 1453, 1561
BsiHKCI CYCGRG 2 cut(s) 17, 971
BsiSI CCGG 6 cut(s) 86, 107, 335, 401, 1231, 1711
BslFI GGGAC 1 cut(s) 220
BslI CCNNNNNNNGG 5 cut(s) 17, 565, 977, 1156, 1554
BsmAI GTCTC 3 cut(s) 95, 796, 1684
BsmBI CGTCTC 1 cut(s) 95
BsmFI GGGAC 1 cut(s) 220
BsnI GGCC 5 cut(s) 95, 356, 491, 1453, 1561
BsoBI CYCGRG 2 cut(s) 17, 971
Bsp143I GATC 4 cut(s) 723, 760, 918, 1306
Bsp1720I GCTNAGC 2 cut(s) 1008, 1533
BspACI CCGC 9 cut(s) 11, 191, 194, 287, 440, 772, 1269, 1443, 1540
BspANI GGCC 5 cut(s) 95, 356, 491, 1453, 1561
BspCNI CTCAG 8 cut(s) 73, 133, 238, 381, 726, 775, 1525, 1555
BspFNI CGCG 1 cut(s) 1423
BspHI TCATGA 1 cut(s) 498
BspLI GGNNCC 1 cut(s) 438
BspMAI CTGCAG 2 cut(s) 211, 875
BspPI GGATC 3 cut(s) 731, 913, 1314
BsrBI CCGCTC 1 cut(s) 1269
BsrDI GCAATG 3 cut(s) 1210, 1254, 1682
BsrFI RCCGGY 1 cut(s) 400
BsrI ACTGG 3 cut(s) 760, 1055, 1498
BssAI RCCGGY 1 cut(s) 400
BssECI CCNNGG 3 cut(s) 96, 559, 972
BssMI GATC 4 cut(s) 723, 760, 918, 1306
BssNAI GTATAC 1 cut(s) 1081
BssNI GRCGYC 2 cut(s) 104, 257
BssSI CACGAG 1 cut(s) 1182
BssT1I CCWWGG 1 cut(s) 559
Bst1107I GTATAC 1 cut(s) 1081
Bst2BI CACGAG 1 cut(s) 1182
Bst2UI CCWGG 1 cut(s) 358
Bst4CI ACNGT 2 cut(s) 1461, 1733
Bst6I CTCTTC 2 cut(s) 685, 858
BstACI GRCGYC 2 cut(s) 104, 257
BstBAI YACGTR 1 cut(s) 302
BstC8I GCNNGC 8 cut(s) 398, 616, 1219, 1290, 1473, 1483, 1530, 1605
BstF5I GGATG 6 cut(s) 897, 982, 1035, 1146, 1333, 1409
BstFNI CGCG 1 cut(s) 1423
BstH2I RGCGCY 1 cut(s) 781
BstHHI GCGC 3 cut(s) 683, 780, 1014
BstKTI GATC 4 cut(s) 726, 763, 921, 1309
BstMAI GTCTC 3 cut(s) 95, 796, 1684
BstMBI GATC 4 cut(s) 723, 760, 918, 1306
BstNI CCWGG 1 cut(s) 358
BstNSI RCATGY 1 cut(s) 1633
BstSCI CCNGG 2 cut(s) 356, 1709
BstSFI CTRYAG 5 cut(s) 207, 871, 1293, 1502, 1744
BstUI CGCG 1 cut(s) 1423
BstX2I RGATCY 2 cut(s) 918, 1306
BstYI RGATCY 2 cut(s) 918, 1306
BstZ17I GTATAC 1 cut(s) 1081
Bsu36I CCTNAGG 1 cut(s) 783
BsuRI GGCC 5 cut(s) 95, 356, 491, 1453, 1561
BtgZI GCGATG 2 cut(s) 1000, 1438
BtsCI GGATG 6 cut(s) 897, 982, 1035, 1146, 1333, 1409
BtsI GCAGTG 1 cut(s) 960
BtsIMutI CAGTG 4 cut(s) 753, 960, 1457, 1738
Cac8I GCNNGC 8 cut(s) 398, 616, 1219, 1290, 1473, 1483, 1530, 1605
CaiI CAGNNNCTG 1 cut(s) 67
CciI TCATGA 1 cut(s) 498
CfoI GCGC 3 cut(s) 683, 780, 1014
Cfr10I RCCGGY 1 cut(s) 400
CseI GACGC 1 cut(s) 112
Csp6I GTAC 3 cut(s) 605, 1045, 1567
CviAII CATG 8 cut(s) 74, 499, 1171, 1222, 1303, 1449, 1623, 1630
CviQI GTAC 3 cut(s) 605, 1045, 1567
DpnI GATC 4 cut(s) 725, 762, 920, 1308
DpnII GATC 4 cut(s) 723, 760, 918, 1306
EaeI YGGCCR 1 cut(s) 1451
Eam1104I CTCTTC 2 cut(s) 685, 858
EarI CTCTTC 2 cut(s) 685, 858
Eco130I CCWWGG 1 cut(s) 559
Eco147I AGGCCT 1 cut(s) 356
Eco57I CTGAAG 1 cut(s) 1704
Eco81I CCTNAGG 1 cut(s) 783
Eco88I CYCGRG 2 cut(s) 17, 971
EcoRI GAATTC 1 cut(s) 305
EcoRII CCWGG 1 cut(s) 356
EcoT14I CCWWGG 1 cut(s) 559
ErhI CCWWGG 1 cut(s) 559
Esp3I CGTCTC 1 cut(s) 95
FaeI CATG 8 cut(s) 77, 502, 1174, 1225, 1306, 1452, 1626, 1633
FalI AAGNNNNNCTT 2 cut(s) 591, 623
FaqI GGGAC 1 cut(s) 220
FatI CATG 8 cut(s) 73, 498, 1170, 1221, 1302, 1448, 1622, 1629
FauI CCCGC 1 cut(s) 765
FbaI TGATCA 1 cut(s) 760
FblI GTMKAC 2 cut(s) 1080, 1715
FokI GGATG 6 cut(s) 904, 989, 1022, 1133, 1340, 1416
GlaI GCGC 3 cut(s) 682, 779, 1013
GsaI CCCAGC 2 cut(s) 1179, 1217
HaeII RGCGCY 1 cut(s) 781
HaeIII GGCC 5 cut(s) 95, 356, 491, 1453, 1561
HapII CCGG 6 cut(s) 86, 107, 335, 401, 1231, 1711
HgaI GACGC 1 cut(s) 112
HhaI GCGC 3 cut(s) 683, 780, 1014
Hin1I GRCGYC 2 cut(s) 104, 257
Hin1II CATG 8 cut(s) 77, 502, 1174, 1225, 1306, 1452, 1626, 1633
Hin6I GCGC 3 cut(s) 681, 778, 1012
HinP1I GCGC 3 cut(s) 681, 778, 1012
HincII GTYRAC 1 cut(s) 1120
HindII GTYRAC 1 cut(s) 1120
HpaII CCGG 6 cut(s) 86, 107, 335, 401, 1231, 1711
HphI GGTGA 2 cut(s) 770, 1352
Hpy166II GTNNAC 4 cut(s) 605, 1081, 1120, 1716
Hpy188III TCNNGA 7 cut(s) 19, 499, 566, 711, 995, 1182, 1618
Hpy8I GTNNAC 4 cut(s) 605, 1081, 1120, 1716
Hpy99I CGWCG 5 cut(s) 94, 220, 259, 262, 265
HpyAV CCTTC 6 cut(s) 181, 386, 427, 502, 593, 1691
HpyCH4III ACNGT 2 cut(s) 1461, 1733
HpyCH4IV ACGT 6 cut(s) 257, 272, 296, 301, 1077, 1124
HpySE526I ACGT 6 cut(s) 257, 272, 296, 301, 1077, 1124
Hsp92I GRCGYC 2 cut(s) 104, 257
Hsp92II CATG 8 cut(s) 77, 502, 1174, 1225, 1306, 1452, 1626, 1633
HspAI GCGC 3 cut(s) 681, 778, 1012
Ksp22I TGATCA 1 cut(s) 760
Kzo9I GATC 4 cut(s) 723, 760, 918, 1306
LmnI GCTCC 8 cut(s) 88, 436, 703, 775, 988, 1318, 1428, 1626
LweI GCATC 4 cut(s) 193, 1023, 1125, 1335
MaeII ACGT 6 cut(s) 257, 272, 296, 301, 1077, 1124
MaeIII GTNAC 3 cut(s) 6, 297, 1406
MalI GATC 4 cut(s) 725, 762, 920, 1308
MbiI CCGCTC 1 cut(s) 1269
MboI GATC 4 cut(s) 723, 760, 918, 1306
MfeI CAATTG 1 cut(s) 684
MflI RGATCY 2 cut(s) 918, 1306
MlsI TGGCCA 1 cut(s) 1453
MluCI AATT 6 cut(s) 305, 532, 684, 807, 1260, 1356
MluNI TGGCCA 1 cut(s) 1453
MlyI GAGTC 7 cut(s) 125, 214, 242, 360, 1151, 1403, 1698
MmeI TCCRAC 1 cut(s) 1225
Mox20I TGGCCA 1 cut(s) 1453
MscI TGGCCA 1 cut(s) 1453
MseI TTAA 3 cut(s) 621, 984, 1062
MslI CAYNNNNRTG 2 cut(s) 946, 1462
Msp20I TGGCCA 1 cut(s) 1453
MspA1I CMGCKG 4 cut(s) 212, 442, 961, 1540
MspI CCGG 6 cut(s) 86, 107, 335, 401, 1231, 1711
MspR9I CCNGG 2 cut(s) 358, 1711
MunI CAATTG 1 cut(s) 684
MvaI CCWGG 1 cut(s) 358
MvnI CGCG 1 cut(s) 1423
NciI CCSGG 1 cut(s) 1711
NdeII GATC 4 cut(s) 723, 760, 918, 1306
NlaIII CATG 8 cut(s) 77, 502, 1174, 1225, 1306, 1452, 1626, 1633
NlaIV GGNNCC 1 cut(s) 438
NmuCI GTSAC 2 cut(s) 6, 1406
NspI RCATGY 1 cut(s) 1633
OliI CACNNNNGTG 1 cut(s) 1462
PagI TCATGA 1 cut(s) 498
PceI AGGCCT 1 cut(s) 356
PcsI WCGNNNNNNNCGW 2 cut(s) 36, 269
PfeI GAWTC 9 cut(s) 70, 317, 329, 374, 425, 635, 707, 714, 1666
PflFI GACNNNGTC 1 cut(s) 259
PleI GAGTC 7 cut(s) 124, 214, 241, 360, 1150, 1403, 1698
PpsI GAGTC 7 cut(s) 124, 214, 241, 360, 1150, 1403, 1698
Ppu21I YACGTR 1 cut(s) 302
Psp6I CCWGG 1 cut(s) 356
PspFI CCCAGC 2 cut(s) 1175, 1213
PspGI CCWGG 1 cut(s) 356
PspN4I GGNNCC 1 cut(s) 438
PstI CTGCAG 2 cut(s) 211, 875
PstNI CAGNNNCTG 1 cut(s) 67
PsuI RGATCY 2 cut(s) 918, 1306
PsyI GACNNNGTC 1 cut(s) 259
PvuII CAGCTG 2 cut(s) 212, 961
RsaI GTAC 3 cut(s) 606, 1046, 1568
RsaNI GTAC 3 cut(s) 605, 1045, 1567
RseI CAYNNNNRTG 2 cut(s) 946, 1462
SaqAI TTAA 3 cut(s) 621, 984, 1062
Sau3AI GATC 4 cut(s) 723, 760, 918, 1306
ScaI AGTACT 2 cut(s) 1046, 1568
SchI GAGTC 7 cut(s) 125, 214, 242, 360, 1151, 1403, 1698
ScrFI CCNGG 2 cut(s) 358, 1711
SfaNI GCATC 4 cut(s) 193, 1023, 1125, 1335
SfcI CTRYAG 5 cut(s) 207, 871, 1293, 1502, 1744
SmiMI CAYNNNNRTG 2 cut(s) 946, 1462
SmlI CTYRAG 1 cut(s) 995
SmoI CTYRAG 1 cut(s) 995
Sse9I AATT 6 cut(s) 305, 532, 684, 807, 1260, 1356
SseBI AGGCCT 1 cut(s) 356
SsiI CCGC 9 cut(s) 11, 191, 194, 287, 440, 772, 1269, 1443, 1540
StuI AGGCCT 1 cut(s) 356
StyD4I CCNGG 2 cut(s) 356, 1709
StyI CCWWGG 1 cut(s) 559
TaaI ACNGT 2 cut(s) 1461, 1733
TaiI ACGT 6 cut(s) 260, 275, 299, 304, 1080, 1127
TaqI TCGA 2 cut(s) 705, 1695
TasI AATT 6 cut(s) 305, 532, 684, 807, 1260, 1356
TatI WGTACW 3 cut(s) 604, 1044, 1566
TauI GCSGC 4 cut(s) 194, 197, 442, 1543
TfiI GAWTC 9 cut(s) 70, 317, 329, 374, 425, 635, 707, 714, 1666
Tru1I TTAA 3 cut(s) 621, 984, 1062
Tru9I TTAA 3 cut(s) 621, 984, 1062
TscAI CASTG 4 cut(s) 760, 960, 1464, 1738
TseFI GTSAC 2 cut(s) 6, 1406
Tsp45I GTSAC 2 cut(s) 6, 1406
TspDTI ATGAA 5 cut(s) 161, 405, 515, 1159, 1437
TspGWI ACGGA 1 cut(s) 1109
TspRI CASTG 4 cut(s) 760, 960, 1464, 1738
Tth111I GACNNNGTC 1 cut(s) 259
XapI RAATTY 2 cut(s) 305, 532
XceI RCATGY 1 cut(s) 1633
XcmI CCANNNNNNNNNTGG 1 cut(s) 1461
XmiI GTMKAC 2 cut(s) 1080, 1715
ZraI GACGTC 1 cut(s) 258
ZrmI AGTACT 2 cut(s) 1046, 1568
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.