MD01G1084000.v1.1

Alkaline and neutral invertase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0008212
Physical Location & Seq
Forward (+)
19037883 .. 19041302
3420 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1084000.v1.1.491

Sequence Viewer

Length: 1983 bp
ATGGCAAAAACGCTCAATGATCTCCTCACCGAAACAAAAGAAGAGGAGGCTGCTCCGCCTCCAGTGCCGGCGACGGTCGAGAAATCTTCCGATGATGTCATCTCGCCGGAAAGGATTCCATGCTCTCCGAACGATGAAGAAGAGCCTGAGGCAAGGCCGTTGGAGAAAGATTCCAATTCTTTGACGAATGAAGGCGTGACATCGAGTTTGAAATCGACAGATGCTTTGGATTACGATCATATTCACCAAATGATTGCGAATTGGAATGCTGAGTCTAAAAGGTCGGTAGAAGAAAGTGCAATAACCTCTGATTCGAGTCCGACAGAGAAGTCGAGCTCGGAGAAGTTTACGGAATCTTTGGATGCTGGCGAGGTGCCAAAGCGGAAGGAAGAGTGCGAGAGACCGCCGGCGAAGAAGACGACAAACTTCCAGCGAAATGACGACGTTCCAGTGAGTGTGAGCGCGGCGAAGCCAGGGGTGCTGTTGCCGTCTCCAAGTGTGGGAGCGAGCCTTATTGAGAACTTGGATCCTTGGAAGGAAGGTTCGCCAAAGACGAAATCGGCAGGGAGCCCAGCGATGGTGGAGGAGGCTTGGGAGAGACTCAAGAAGTCGTACGTATACTTCAAAGGCAAGCCAGTGGGTACTCTTGCAGCTATGGATCCAACGGCTGAGACTTTGAATTACAATCAGGTGTTTGTGAGAGATTTTGTTCCTAGTGGTTTGGCCTGCCTGATGAAAAAAGAACCAGATCAACGAGATGTTGTAAAGAATTTTCTCCTCAAGACCCTCCACCTCCAAAGCTGGGAAAAGAGGATCGATAATTTTACACTTGGAGAAGGTGTTCTGCCGGCAAGTTTTAAGGTTCTCTTTGATCAACATCGTAAGAATGAAACTCTAGTTGCGGATTTTGGTGGCAGCGCAATTGGAAGAGTTGCGCCTATTGATTCTGGGTTCTGGTGGATCATTTTGCTGAGATCATACACCAAATGCACTCGTGATCACTCACTGGCAGAACTTCCTGAGGTGCAGACGGGAATAAAGTTAATTCTCAATCTTTGCCTGTCAGATGGGTTTGACACATTCCCAACACTTCTCTGTGCAGATGGGTGTAGTATGATCGACAGGAGGATGGGGATGTATGGTTATCCAATTGAGATTCAAGCACTCTTCTACTTTGCATTGAGGTGTGCCCGGCAGCTGTTGAATCCAGAACGTGGTGGCAAGGAATTCCTTGAGCGGATAGATAAGCGCATCTCAGCTCTCAGCTTTCACATCCAGACGTATTACTGGCTTGATTTTACACAGCTAAACAATGTGTACCGCTACAAAACTGAGGAATATTCCCATACTGCCGTCAATAAGTTTAATGTCATTCCAGAGTCTATCCCTGACTGGGTTTTCGATTTTATGCCCTTGCGAGGAGGATATCTGATCGGCAATGTTAGCCCAGCTAGGATGGACTTCAGGTGGTTTTTAGTTGGGAATTGTATTGCCATCCTCAGCTCGCTGGTAACACCTGTGCAAGCTACAGCAATTATGGACTTGATTGAGGAGAGATGGGAGGACTTGATCGGGGAGATGCCTCTGAAAATTATTTACCCTGCATTGGAGGGACATGAGTGGAGAATTGTCACGGGATTTGATCCGAAAAACACACGATGGAGTTATCACAATGGTGGTTCTTGGCCAACGCTGCTGTGGCTGCTTACCGCAGCTTGCATCAAGACCGGGAGGCCTCAGATGGCGAAGAAAGCAATCGAGCTGGTGGAGCAGCGACTCTCGAAAGACGGGTGGCCCGAGTACTATGACGGCAAGACAGGGAGGTACATTGGGAAGCAAGCAAGAAAGTACCAGACTTGGAGTATCTCCGGATACCTGGTTGCTAAGTTGATGATCGAGAATCCGGCCAATCTTGCACTCATCTCTCTTGAAGAGGACAAGAAGATAACCAAGCCAAGGCTTACCCGCTCTGCTTCTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_100

Protein Analysis

661

Amino Acids

74.46

Weight (kDa)

5.66

Isoelectric Point (pI)

52.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_100 PF12899 197 - 635 8.7e-214 Alkaline and neutral invertase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 328
AccB1I GGYRCC 1 cut(s) 373
AccB7I CCANNNNNTGG 1 cut(s) 1214
AccBSI CCGCTC 2 cut(s) 1237, 1968
AccI GTMKAC 1 cut(s) 618
AccII CGCG 1 cut(s) 464
AccIII TCCGGA 1 cut(s) 1868
AciI CCGC 9 cut(s) 56, 382, 404, 464, 902, 1237, 1321, 1710, 1966
AclWI GGATC 7 cut(s) 521, 534, 653, 666, 821, 968, 1637
AcoI YGGCCR 2 cut(s) 1685, 1905
AcsI RAATTY 2 cut(s) 769, 1226
AcuI CTGAAG 1 cut(s) 1447
AfaI GTAC 6 cut(s) 614, 643, 1319, 1802, 1826, 1850
AfiI CCNNNNNNNGG 8 cut(s) 500, 577, 802, 1214, 1393, 1418, 1606, 1956
AgsI TTSAA 6 cut(s) 211, 625, 679, 1160, 1204, 1931
AjnI CCWGG 2 cut(s) 472, 1875
AleI CACNNNNGTG 1 cut(s) 1674
AloI GAACNNNNNNTCC 4 cut(s) 526, 558, 825, 857
Alw21I GWGCWC 1 cut(s) 338
Alw26I GTCTC 4 cut(s) 394, 495, 592, 665
AlwI GGATC 7 cut(s) 521, 534, 653, 666, 821, 968, 1637
Ama87I CYCGRG 1 cut(s) 1796
Aor13HI TCCGGA 1 cut(s) 1868
AoxI GGCC 6 cut(s) 155, 723, 1685, 1733, 1793, 1905
ApeKI GCWGC 8 cut(s) 50, 650, 915, 1195, 1693, 1702, 1712, 1771
ApoI RAATTY 2 cut(s) 769, 1226
ArsI GACNNNNNNTTYG 6 cut(s) 190, 208, 222, 240, 1382, 1414
Asp700I GAANNNNTTC 2 cut(s) 114, 840
AspLEI GCGC 4 cut(s) 464, 920, 937, 1251
AspS9I GGNCC 1 cut(s) 1794
AsuC2I CCSGG 2 cut(s) 1192, 1729
AsuHPI GGTGA 2 cut(s) 19, 236
AvaI CYCGRG 1 cut(s) 1796
AxyI CCTNAGG 2 cut(s) 147, 1020
BaeGI GKGCMC 1 cut(s) 1192
BalI TGGCCA 1 cut(s) 1687
BamHI GGATCC 2 cut(s) 526, 658
BanI GGYRCC 1 cut(s) 373
BanII GRGCYC 2 cut(s) 338, 572
BarI GAAGNNNNNNTAC 2 cut(s) 605, 637
BauI CACGAG 1 cut(s) 993
BbsI GAAGAC 1 cut(s) 422
Bbv12I GWGCWC 1 cut(s) 338
BbvCI CCTCAGC 1 cut(s) 1499
BbvI GCAGC 8 cut(s) 37, 662, 927, 1207, 1680, 1689, 1724, 1783
BccI CCATC 9 cut(s) 571, 1061, 1097, 1123, 1450, 1502, 1551, 1653, 1735
BceAI ACGGC 5 cut(s) 142, 472, 681, 1337, 1825
BciT130I CCWGG 2 cut(s) 474, 1877
BciVI GTATCC 1 cut(s) 1865
BclI TGATCA 2 cut(s) 871, 997
BcnI CCSGG 2 cut(s) 1192, 1729
BcoDI GTCTC 4 cut(s) 394, 495, 592, 665
BfaI CTAG 3 cut(s) 714, 896, 1452
BfmI CTRYAG 1 cut(s) 1527
BfuI GTATCC 1 cut(s) 1865
BisI GCNGC 9 cut(s) 51, 465, 651, 916, 1196, 1694, 1703, 1713, 1772
BlsI GCNGC 9 cut(s) 52, 466, 652, 917, 1197, 1695, 1704, 1714, 1773
BmcAI AGTACT 1 cut(s) 1802
Bme1390I CCNGG 4 cut(s) 474, 1192, 1729, 1877
BmeT110I CYCGRG 1 cut(s) 1796
BmgT120I GGNCC 1 cut(s) 1794
BmiI GGNNCC 4 cut(s) 375, 528, 569, 660
BmrFI CCNGG 4 cut(s) 474, 1192, 1729, 1877
BmrI ACTGGG 1 cut(s) 1402
BmsI GCATC 5 cut(s) 211, 352, 1260, 1569, 1728
BmuI ACTGGG 1 cut(s) 1402
BpiI GAAGAC 1 cut(s) 422
BpmI CTGGAG 1 cut(s) 45
Bpu10I CCTNAGC 1 cut(s) 1499
BpuEI CTTGAG 3 cut(s) 587, 764, 1253
BpuMI CCSGG 2 cut(s) 1192, 1729
Bsa29I ATCGAT 1 cut(s) 816
BsaAI YACGTR 1 cut(s) 616
BsaBI GATNNNNATC 2 cut(s) 234, 876
BsaI GGTCTC 1 cut(s) 394
BsaJI CCNNGG 3 cut(s) 473, 530, 1955
BsaWI WCCGGW 1 cut(s) 1868
BsaXI ACNNNNNCTCC 2 cut(s) 825, 855
Bsc4I CCNNNNNNNGG 8 cut(s) 500, 577, 802, 1214, 1393, 1418, 1606, 1956
Bse118I RCCGGY 3 cut(s) 67, 406, 847
Bse1I ACTGG 6 cut(s) 62, 449, 635, 1011, 1292, 1397
Bse21I CCTNAGG 2 cut(s) 147, 1020
Bse3DI GCAATG 1 cut(s) 1444
Bse8I GATNNNNATC 2 cut(s) 234, 876
BseAI TCCGGA 1 cut(s) 1868
BseBI CCWGG 2 cut(s) 474, 1877
BseCI ATCGAT 1 cut(s) 816
BseDI CCNNGG 3 cut(s) 473, 530, 1955
BseGI GGATG 6 cut(s) 367, 1134, 1140, 1272, 1461, 1494
BseJI GATNNNNATC 2 cut(s) 234, 876
BseLI CCNNNNNNNGG 8 cut(s) 500, 577, 802, 1214, 1393, 1418, 1606, 1956
BseMI GCAATG 1 cut(s) 1444
BseNI ACTGG 6 cut(s) 62, 449, 635, 1011, 1292, 1397
BseRI GAGGAG 6 cut(s) 14, 59, 599, 767, 1434, 1565
BseSI GKGCMC 1 cut(s) 1192
BseXI GCAGC 8 cut(s) 37, 662, 927, 1207, 1680, 1689, 1724, 1783
BseYI CCCAGC 3 cut(s) 571, 801, 1447
BsgI GTGCAG 2 cut(s) 1046, 1119
Bsh1236I CGCG 1 cut(s) 464
Bsh1285I CGRYCG 1 cut(s) 78
BshFI GGCC 6 cut(s) 157, 725, 1687, 1735, 1795, 1907
BshNI GGYRCC 1 cut(s) 373
BshVI ATCGAT 1 cut(s) 816
BsiEI CGRYCG 1 cut(s) 78
BsiHKAI GWGCWC 1 cut(s) 338
BsiHKCI CYCGRG 1 cut(s) 1796
BsiSI CCGG 8 cut(s) 68, 107, 407, 848, 1192, 1728, 1869, 1904
BsiWI CGTACG 1 cut(s) 612
BslFI GGGAC 1 cut(s) 1626
BslI CCNNNNNNNGG 8 cut(s) 500, 577, 802, 1214, 1393, 1418, 1606, 1956
BsmAI GTCTC 4 cut(s) 394, 495, 592, 665
BsmBI CGTCTC 1 cut(s) 495
BsmFI GGGAC 1 cut(s) 1626
BsmI GAATGC 1 cut(s) 271
BsnI GGCC 6 cut(s) 157, 725, 1687, 1735, 1795, 1907
Bso31I GGTCTC 1 cut(s) 394
BsoBI CYCGRG 1 cut(s) 1796
Bsp1286I GDGCHC 3 cut(s) 338, 572, 1192
Bsp13I TCCGGA 1 cut(s) 1868
BspACI CCGC 9 cut(s) 56, 382, 404, 464, 902, 1237, 1321, 1710, 1966
BspANI GGCC 6 cut(s) 157, 725, 1687, 1735, 1795, 1907
BspDI ATCGAT 1 cut(s) 816
BspEI TCCGGA 1 cut(s) 1868
BspFNI CGCG 1 cut(s) 464
BspLI GGNNCC 4 cut(s) 375, 528, 569, 660
BspPI GGATC 7 cut(s) 521, 534, 653, 666, 821, 968, 1637
BspQI GCTCTTC 1 cut(s) 135
BspT107I GGYRCC 1 cut(s) 373
BspTNI GGTCTC 1 cut(s) 394
BsrBI CCGCTC 2 cut(s) 1237, 1968
BsrDI GCAATG 1 cut(s) 1444
BsrFI RCCGGY 3 cut(s) 67, 406, 847
BsrI ACTGG 6 cut(s) 62, 449, 635, 1011, 1292, 1397
BssAI RCCGGY 3 cut(s) 67, 406, 847
BssECI CCNNGG 3 cut(s) 473, 530, 1955
BssNAI GTATAC 1 cut(s) 619
BssSI CACGAG 1 cut(s) 993
BssT1I CCWWGG 2 cut(s) 530, 1955
Bst1107I GTATAC 1 cut(s) 619
Bst2BI CACGAG 1 cut(s) 993
Bst2UI CCWGG 2 cut(s) 474, 1877
Bst4CI ACNGT 1 cut(s) 76
Bst6I CTCTTC 6 cut(s) 36, 135, 384, 922, 1172, 1926
BstBAI YACGTR 1 cut(s) 616
BstF5I GGATG 6 cut(s) 367, 1134, 1140, 1272, 1461, 1494
BstFNI CGCG 1 cut(s) 464
BstHHI GCGC 4 cut(s) 464, 920, 937, 1251
BstMAI GTCTC 4 cut(s) 394, 495, 592, 665
BstMCI CGRYCG 1 cut(s) 78
BstMWI GCNNNNNNNGC 9 cut(s) 64, 478, 1443, 1693, 1699, 1702, 1751, 1768, 1913
BstNI CCWGG 2 cut(s) 474, 1877
BstSCI CCNGG 4 cut(s) 472, 1190, 1727, 1875
BstSFI CTRYAG 1 cut(s) 1527
BstSLI GKGCMC 1 cut(s) 1192
BstSNI TACGTA 1 cut(s) 616
BstUI CGCG 1 cut(s) 464
BstV1I GCAGC 8 cut(s) 37, 662, 927, 1207, 1680, 1689, 1724, 1783
BstV2I GAAGAC 1 cut(s) 422
BstX2I RGATCY 2 cut(s) 526, 658
BstYI RGATCY 2 cut(s) 526, 658
BstZ17I GTATAC 1 cut(s) 619
Bsu15I ATCGAT 1 cut(s) 816
Bsu36I CCTNAGG 2 cut(s) 147, 1020
BsuI GTATCC 1 cut(s) 1865
BsuRI GGCC 6 cut(s) 157, 725, 1687, 1735, 1795, 1907
BsuTUI ATCGAT 1 cut(s) 816
BtgZI GCGATG 1 cut(s) 590
BtsCI GGATG 6 cut(s) 367, 1134, 1140, 1272, 1461, 1494
BtsIMutI CAGTG 4 cut(s) 69, 456, 642, 1004
CfoI GCGC 4 cut(s) 464, 920, 937, 1251
Cfr10I RCCGGY 3 cut(s) 67, 406, 847
Cfr13I GGNCC 1 cut(s) 1794
ClaI ATCGAT 1 cut(s) 816
CsiI ACCWGGT 1 cut(s) 1875
Csp6I GTAC 6 cut(s) 613, 642, 1318, 1801, 1825, 1849
CviAII CATG 2 cut(s) 120, 1616
CviQI GTAC 6 cut(s) 613, 642, 1318, 1801, 1825, 1849
DrdI GACNNNNNNGTC 1 cut(s) 328
DseDI GACNNNNNNGTC 1 cut(s) 328
EaeI YGGCCR 2 cut(s) 1685, 1905
Eam1104I CTCTTC 6 cut(s) 36, 135, 384, 922, 1172, 1926
EarI CTCTTC 6 cut(s) 36, 135, 384, 922, 1172, 1926
EciI GGCGGA 1 cut(s) 45
Ecl136II GAGCTC 1 cut(s) 336
Eco105I TACGTA 1 cut(s) 616
Eco130I CCWWGG 2 cut(s) 530, 1955
Eco147I AGGCCT 1 cut(s) 1735
Eco24I GRGCYC 2 cut(s) 338, 572
Eco31I GGTCTC 1 cut(s) 394
Eco32I GATATC 1 cut(s) 1427
Eco53kI GAGCTC 1 cut(s) 336
Eco57I CTGAAG 1 cut(s) 1447
Eco81I CCTNAGG 2 cut(s) 147, 1020
Eco88I CYCGRG 1 cut(s) 1796
EcoICRI GAGCTC 1 cut(s) 336
EcoRI GAATTC 1 cut(s) 1226
EcoRII CCWGG 2 cut(s) 472, 1875
EcoRV GATATC 1 cut(s) 1427
EcoT14I CCWWGG 2 cut(s) 530, 1955
EcoT38I GRGCYC 2 cut(s) 338, 572
ErhI CCWWGG 2 cut(s) 530, 1955
Esp3I CGTCTC 1 cut(s) 495
FaeI CATG 2 cut(s) 123, 1619
FalI AAGNNNNNCTT 2 cut(s) 851, 883
FaqI GGGAC 1 cut(s) 1626
FatI CATG 2 cut(s) 119, 1615
FauI CCCGC 1 cut(s) 1973
FbaI TGATCA 2 cut(s) 871, 997
FblI GTMKAC 1 cut(s) 618
Fnu4HI GCNGC 9 cut(s) 51, 465, 651, 916, 1196, 1694, 1703, 1713, 1772
FokI GGATG 6 cut(s) 374, 1141, 1147, 1259, 1468, 1481
FriOI GRGCYC 2 cut(s) 338, 572
Fsp4HI GCNGC 9 cut(s) 51, 465, 651, 916, 1196, 1694, 1703, 1713, 1772
FspBI CTAG 3 cut(s) 714, 896, 1452
GlaI GCGC 4 cut(s) 463, 919, 936, 1250
GluI GCNGC 9 cut(s) 51, 465, 651, 916, 1196, 1694, 1703, 1713, 1772
GsaI CCCAGC 3 cut(s) 575, 805, 1451
GsuI CTGGAG 1 cut(s) 45
HaeIII GGCC 6 cut(s) 157, 725, 1687, 1735, 1795, 1907
HapII CCGG 8 cut(s) 68, 107, 407, 848, 1192, 1728, 1869, 1904
HhaI GCGC 4 cut(s) 464, 920, 937, 1251
Hin1II CATG 2 cut(s) 123, 1619
Hin6I GCGC 4 cut(s) 462, 918, 935, 1249
HinP1I GCGC 4 cut(s) 462, 918, 935, 1249
HpaII CCGG 8 cut(s) 68, 107, 407, 848, 1192, 1728, 1869, 1904
HphI GGTGA 2 cut(s) 19, 236
Hpy166II GTNNAC 3 cut(s) 348, 619, 1318
Hpy8I GTNNAC 3 cut(s) 348, 619, 1318
Hpy99I CGWCG 2 cut(s) 76, 446
HpyAV CCTTC 5 cut(s) 185, 379, 529, 533, 830
HpyCH4III ACNGT 1 cut(s) 76
HpyCH4IV ACGT 4 cut(s) 444, 615, 1213, 1280
HpyF10VI GCNNNNNNNGC 9 cut(s) 64, 478, 1443, 1693, 1699, 1702, 1751, 1768, 1913
HpySE526I ACGT 4 cut(s) 444, 615, 1213, 1280
Hsp92II CATG 2 cut(s) 123, 1619
HspAI GCGC 4 cut(s) 462, 918, 935, 1249
Kpn2I TCCGGA 1 cut(s) 1868
KroI GCCGGC 3 cut(s) 67, 406, 847
KroNI GCCGGC 3 cut(s) 69, 408, 849
Ksp22I TGATCA 2 cut(s) 871, 997
LguI GCTCTTC 1 cut(s) 135
LmnI GCTCC 4 cut(s) 58, 503, 567, 1768
Lsp1109I GCAGC 8 cut(s) 37, 662, 927, 1207, 1680, 1689, 1724, 1783
LweI GCATC 5 cut(s) 211, 352, 1260, 1569, 1728
MabI ACCWGGT 1 cut(s) 1875
MaeI CTAG 3 cut(s) 714, 896, 1452
MaeII ACGT 4 cut(s) 444, 615, 1213, 1280
MaeIII GTNAC 3 cut(s) 196, 1510, 1630
MbiI CCGCTC 2 cut(s) 1237, 1968
MfeI CAATTG 2 cut(s) 921, 1149
MflI RGATCY 2 cut(s) 526, 658
MhlI GDGCHC 3 cut(s) 338, 572, 1192
MlsI TGGCCA 1 cut(s) 1687
MluNI TGGCCA 1 cut(s) 1687
MlyI GAGTC 5 cut(s) 281, 325, 594, 1388, 1770
MmeI TCCRAC 3 cut(s) 141, 344, 686
Mox20I TGGCCA 1 cut(s) 1687
MreI CGCCGGCG 1 cut(s) 406
MroI TCCGGA 1 cut(s) 1868
MroNI GCCGGC 3 cut(s) 67, 406, 847
MroXI GAANNNNTTC 2 cut(s) 114, 840
MscI TGGCCA 1 cut(s) 1687
MseI TTAA 3 cut(s) 858, 1043, 1365
MslI CAYNNNNRTG 2 cut(s) 1183, 1674
Msp20I TGGCCA 1 cut(s) 1687
MspA1I CMGCKG 1 cut(s) 1198
MspI CCGG 8 cut(s) 68, 107, 407, 848, 1192, 1728, 1869, 1904
MspR9I CCNGG 4 cut(s) 474, 1192, 1729, 1877
MunI CAATTG 2 cut(s) 921, 1149
Mva1269I GAATGC 1 cut(s) 271
MvaI CCWGG 2 cut(s) 474, 1877
MvnI CGCG 1 cut(s) 464
MwoI GCNNNNNNNGC 9 cut(s) 64, 478, 1443, 1693, 1699, 1702, 1751, 1768, 1913
NaeI GCCGGC 3 cut(s) 69, 408, 849
NciI CCSGG 2 cut(s) 1192, 1729
NgoMIV GCCGGC 3 cut(s) 67, 406, 847
NlaIII CATG 2 cut(s) 123, 1619
NlaIV GGNNCC 4 cut(s) 375, 528, 569, 660
NmuCI GTSAC 2 cut(s) 196, 1630
OliI CACNNNNGTG 1 cut(s) 1674
PceI AGGCCT 1 cut(s) 1735
PciSI GCTCTTC 1 cut(s) 135
PcsI WCGNNNNNNNCGW 2 cut(s) 551, 1794
PctI GAATGC 1 cut(s) 271
PdiI GCCGGC 3 cut(s) 69, 408, 849
PdmI GAANNNNTTC 2 cut(s) 114, 840
PfeI GAWTC 8 cut(s) 115, 170, 311, 353, 944, 1156, 1204, 1900
Pfl23II CGTACG 1 cut(s) 612
PflMI CCANNNNNTGG 1 cut(s) 1214
PkrI GCNGC 9 cut(s) 52, 466, 652, 917, 1197, 1695, 1704, 1714, 1773
PleI GAGTC 5 cut(s) 280, 324, 594, 1387, 1770
PpsI GAGTC 5 cut(s) 280, 324, 594, 1387, 1770
Ppu21I YACGTR 1 cut(s) 616
Psp124BI GAGCTC 1 cut(s) 338
Psp6I CCWGG 2 cut(s) 472, 1875
PspFI CCCAGC 3 cut(s) 571, 801, 1447
PspGI CCWGG 2 cut(s) 472, 1875
PspLI CGTACG 1 cut(s) 612
PspN4I GGNNCC 4 cut(s) 375, 528, 569, 660
PspPI GGNCC 1 cut(s) 1794
PsuI RGATCY 2 cut(s) 526, 658
PvuII CAGCTG 1 cut(s) 1198
RsaI GTAC 6 cut(s) 614, 643, 1319, 1802, 1826, 1850
RsaNI GTAC 6 cut(s) 613, 642, 1318, 1801, 1825, 1849
RseI CAYNNNNRTG 2 cut(s) 1183, 1674
SacI GAGCTC 1 cut(s) 338
SapI GCTCTTC 1 cut(s) 135
SaqAI TTAA 3 cut(s) 858, 1043, 1365
SatI GCNGC 9 cut(s) 51, 465, 651, 916, 1196, 1694, 1703, 1713, 1772
Sau96I GGNCC 1 cut(s) 1794
ScaI AGTACT 1 cut(s) 1802
SchI GAGTC 5 cut(s) 281, 325, 594, 1388, 1770
ScrFI CCNGG 4 cut(s) 474, 1192, 1729, 1877
SduI GDGCHC 3 cut(s) 338, 572, 1192
SexAI ACCWGGT 1 cut(s) 1875
SfaNI GCATC 5 cut(s) 211, 352, 1260, 1569, 1728
SfcI CTRYAG 1 cut(s) 1527
SgrAI CRCCGGYG 1 cut(s) 406
SmiMI CAYNNNNRTG 2 cut(s) 1183, 1674
SmlI CTYRAG 3 cut(s) 602, 779, 1232
SmoI CTYRAG 3 cut(s) 602, 779, 1232
SnaBI TACGTA 1 cut(s) 616
SseBI AGGCCT 1 cut(s) 1735
SsiI CCGC 9 cut(s) 56, 382, 404, 464, 902, 1237, 1321, 1710, 1966
SspI AATATT 1 cut(s) 1340
SspMI CTAG 3 cut(s) 714, 896, 1452
SstI GAGCTC 1 cut(s) 338
StuI AGGCCT 1 cut(s) 1735
StyD4I CCNGG 4 cut(s) 472, 1190, 1727, 1875
StyI CCWWGG 2 cut(s) 530, 1955
TaaI ACNGT 1 cut(s) 76
TaiI ACGT 4 cut(s) 447, 618, 1216, 1283
TatI WGTACW 1 cut(s) 1800
TauI GCSGC 1 cut(s) 467
TfiI GAWTC 8 cut(s) 115, 170, 311, 353, 944, 1156, 1204, 1900
Tru1I TTAA 3 cut(s) 858, 1043, 1365
Tru9I TTAA 3 cut(s) 858, 1043, 1365
TscAI CASTG 4 cut(s) 69, 456, 642, 1011
TseFI GTSAC 2 cut(s) 196, 1630
TseI GCWGC 8 cut(s) 50, 650, 915, 1195, 1693, 1702, 1712, 1771
Tsp45I GTSAC 2 cut(s) 196, 1630
TspDTI ATGAA 4 cut(s) 150, 204, 749, 903
TspGWI ACGGA 1 cut(s) 365
TspRI CASTG 4 cut(s) 69, 456, 642, 1011
Van91I CCANNNNNTGG 1 cut(s) 1214
XapI RAATTY 2 cut(s) 769, 1226
XcmI CCANNNNNNNNNTGG 1 cut(s) 1695
XmiI GTMKAC 1 cut(s) 618
XmnI GAANNNNTTC 2 cut(s) 114, 840
XspI CTAG 3 cut(s) 714, 896, 1452
ZrmI AGTACT 1 cut(s) 1802
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.