FvH4_7g21580

Alkaline and neutral invertase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0008212
Physical Location & Seq
Forward (+)
17234479 .. 17236915
2437 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g21580.t1

Sequence Viewer

Length: 1227 bp
ATGTCGACGTCACCGCTGCTCAAGAAGGAAATCGAAGCACAGGAGAAGGCTTCTCAGCCTCCTAATCATGCTCCTGGGCCGGCGACTGCGGACTCTGTGCCGGAGATAGAGTCGCCGATGATGCTTTCGCCGGTGATAAAGAAAAAGAAGGATGCTTCTGGCTCTTCCCACACTGGAGCTCGAACCTTTGTGAAGGGCGAGAGTTCCGAAACGACGTCGTTGTTCCATGATTTCATCGTGGAATCTTTGGAGGAATCTGCGAAGACTATGGTGGACGAGGGCTGGAGGCGACTCGGGGAGTCTGTCGTTTTCTATCAAGGCAAACCGGTTGGGACTCTTCAGGCTATGGATCAAGTGGGCAATCAGGCATCTGTGAGGGATTTTGTTCCTGCTGGTTTGGCCTTCCTCATGAAGAAACCAGCAGAGCCAGGGGTAGTGAAGAATTTTCTCTTGAAGACACTCCACCTCCAAGGTCAGGAGAAAAAGATTGATAACTTCACAGTCGGAGAAGGTGTGCTTCCTGCGAGCTTTAAGGTCTCTTCCGATTCCTGTGGTGGCAAGAAAGCCTTTGTTGCAGATTTCAGTGGTAGCGCAATTGGAAGAGTTGCTCCTGTCGATTCTAGATTGTCGCTGAGGGCATATACTACATGCACTGGTTATCATACGCTGCAGAAGCGCCCTGAGCTGCAAAAGCCAATGAGTTTGAGCCTTTCAGATGACTTTGACACATTTCCAACTCTTATTTGTGGAGATGATTGCAGTATGATCGACATGAAGATGGGCCTTAGCAATGATTTGATTGGGGAGATGCCTCTGAAAATTGCATATCCAGCATTAGAGGGATATCAGCGGAAGACAAGCACAGGATCTGACAATGCTGCAACTCTGTCCAGAAAGCGCAAACGTGAGGATGAAGATCCGAAGTCTGCCAAACAGCAGATTAAATTGTTTCTGAACCCAACACGGCCTGTGGATGTCTTTTGGTGTGATGGACGTGGCACCATAACGTTTCTCGGTCATATACAAAATGTCCCGTTTATGCGTAATGTGCTTGAGCTTGAGATACAAGTCAATATCGTTGCGTACAGCCACCAGCACTACAATGAGGAACAGAGGTTATACTACAGGCGGTGGACAACTGAAGGTATTGTTGAGATATCTGTAGATGAACAGTGGTTGGAATTTCGAAGGGCTGTGAGGGGTGTTATTCTATTTGAGGCACCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_100

Protein Analysis

409

Amino Acids

45.3

Weight (kDa)

6.48

Isoelectric Point (pI)

53.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_100 PF12899 95 - 262 5.9e-41 Alkaline and neutral invertase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 11, 218
AccB1I GGYRCC 2 cut(s) 998, 1219
AccI GTMKAC 1 cut(s) 5
AciI CCGC 4 cut(s) 14, 89, 850, 1129
AclI AACGTT 1 cut(s) 1007
AclWI GGATC 3 cut(s) 357, 874, 911
AcsI RAATTY 2 cut(s) 442, 1181
AcuI CTGAAG 2 cut(s) 323, 1161
AcyI GRCGYC 2 cut(s) 8, 215
AfaI GTAC 1 cut(s) 1085
AfiI CCNNNNNNNGG 1 cut(s) 475
AgeI ACCGGT 1 cut(s) 325
AgsI TTSAA 1 cut(s) 454
AjiI CACGTC 1 cut(s) 995
AjnI CCWGG 2 cut(s) 73, 427
AluBI AGCT 4 cut(s) 179, 528, 685, 1057
AluI AGCT 4 cut(s) 179, 528, 685, 1057
Alw21I GWGCWC 1 cut(s) 181
Alw26I GTCTC 1 cut(s) 541
AlwI GGATC 3 cut(s) 357, 874, 911
AlwNI CAGNNNCTG 1 cut(s) 869
Ama87I CYCGRG 1 cut(s) 293
AoxI GGCC 4 cut(s) 77, 399, 781, 965
ApeKI GCWGC 4 cut(s) 16, 667, 685, 878
ApoI RAATTY 2 cut(s) 442, 1181
AsiGI ACCGGT 1 cut(s) 325
AspLEI GCGC 3 cut(s) 593, 678, 900
AspS9I GGNCC 2 cut(s) 77, 781
AsuHPI GGTGA 2 cut(s) 3, 145
AsuII TTCGAA 1 cut(s) 1186
AvaI CYCGRG 1 cut(s) 293
BanI GGYRCC 2 cut(s) 998, 1219
BanII GRGCYC 1 cut(s) 181
BbsI GAAGAC 3 cut(s) 269, 461, 860
Bbv12I GWGCWC 1 cut(s) 181
BbvCI CCTCAGC 1 cut(s) 632
BbvI GCAGC 4 cut(s) 3, 654, 672, 865
BccI CCATC 2 cut(s) 772, 983
BceAI ACGGC 1 cut(s) 980
BcgI CGANNNNNNTGC 2 cut(s) 748, 782
BciT130I CCWGG 2 cut(s) 75, 429
BcoDI GTCTC 1 cut(s) 541
BfaI CTAG 1 cut(s) 621
BfmI CTRYAG 3 cut(s) 668, 1123, 1161
BfoI RGCGCY 1 cut(s) 679
BisI GCNGC 4 cut(s) 17, 668, 686, 879
BlsI GCNGC 4 cut(s) 18, 669, 687, 880
Bme1390I CCNGG 2 cut(s) 75, 429
BmeT110I CYCGRG 1 cut(s) 293
BmgBI CACGTC 1 cut(s) 995
BmgT120I GGNCC 2 cut(s) 77, 781
BmiI GGNNCC 2 cut(s) 1000, 1221
BmrFI CCNGG 2 cut(s) 75, 429
BmsI GCATC 4 cut(s) 111, 142, 377, 798
BpiI GAAGAC 3 cut(s) 269, 461, 860
BpmI CTGGAG 2 cut(s) 195, 304
Bpu10I CCTNAGC 3 cut(s) 632, 681, 785
Bpu14I TTCGAA 1 cut(s) 1186
BpuEI CTTGAG 3 cut(s) 5, 1073, 1079
BsaBI GATNNNNATC 1 cut(s) 915
BsaHI GRCGYC 2 cut(s) 8, 215
BsaI GGTCTC 1 cut(s) 541
BsaJI CCNNGG 3 cut(s) 74, 428, 469
BsaWI WCCGGW 1 cut(s) 325
BsaXI ACNNNNNCTCC 6 cut(s) 95, 125, 450, 480, 498, 528
Bsc4I CCNNNNNNNGG 1 cut(s) 475
Bse118I RCCGGY 3 cut(s) 79, 130, 325
Bse1I ACTGG 2 cut(s) 178, 658
Bse3DI GCAATG 1 cut(s) 796
Bse8I GATNNNNATC 1 cut(s) 915
BseBI CCWGG 2 cut(s) 75, 429
BseDI CCNNGG 3 cut(s) 74, 428, 469
BseGI GGATG 3 cut(s) 157, 916, 979
BseJI GATNNNNATC 1 cut(s) 915
BseLI CCNNNNNNNGG 1 cut(s) 475
BseMI GCAATG 1 cut(s) 796
BseMII CTCAG 3 cut(s) 68, 623, 672
BseNI ACTGG 2 cut(s) 178, 658
BseXI GCAGC 4 cut(s) 3, 654, 672, 865
BshFI GGCC 4 cut(s) 79, 401, 783, 967
BshNI GGYRCC 2 cut(s) 998, 1219
BshTI ACCGGT 1 cut(s) 325
BsiHKAI GWGCWC 1 cut(s) 181
BsiHKCI CYCGRG 1 cut(s) 293
BsiSI CCGG 4 cut(s) 80, 101, 131, 326
BslFI GGGAC 2 cut(s) 346, 1016
BslI CCNNNNNNNGG 1 cut(s) 475
BsmAI GTCTC 1 cut(s) 541
BsmFI GGGAC 2 cut(s) 346, 1016
BsnI GGCC 4 cut(s) 79, 401, 783, 967
Bso31I GGTCTC 1 cut(s) 541
BsoBI CYCGRG 1 cut(s) 293
Bsp119I TTCGAA 1 cut(s) 1186
Bsp1286I GDGCHC 1 cut(s) 181
Bsp143I GATC 4 cut(s) 349, 765, 866, 916
BspACI CCGC 4 cut(s) 14, 89, 850, 1129
BspANI GGCC 4 cut(s) 79, 401, 783, 967
BspCNI CTCAG 3 cut(s) 67, 624, 673
BspHI TCATGA 1 cut(s) 408
BspLI GGNNCC 2 cut(s) 1000, 1221
BspMAI CTGCAG 1 cut(s) 672
BspPI GGATC 3 cut(s) 357, 874, 911
BspQI GCTCTTC 1 cut(s) 169
BspT104I TTCGAA 1 cut(s) 1186
BspT107I GGYRCC 2 cut(s) 998, 1219
BspTNI GGTCTC 1 cut(s) 541
BsrDI GCAATG 1 cut(s) 796
BsrFI RCCGGY 3 cut(s) 79, 130, 325
BsrI ACTGG 2 cut(s) 178, 658
BssAI RCCGGY 3 cut(s) 79, 130, 325
BssECI CCNNGG 3 cut(s) 74, 428, 469
BssMI GATC 4 cut(s) 349, 765, 866, 916
BssNI GRCGYC 2 cut(s) 8, 215
BssT1I CCWWGG 1 cut(s) 469
Bst2UI CCWGG 2 cut(s) 75, 429
Bst4CI ACNGT 2 cut(s) 502, 1173
Bst6I CTCTTC 4 cut(s) 169, 342, 544, 595
BstACI GRCGYC 2 cut(s) 8, 215
BstBI TTCGAA 1 cut(s) 1186
BstC8I GCNNGC 2 cut(s) 81, 526
BstDEI CTNAG 4 cut(s) 54, 632, 681, 785
BstF5I GGATG 3 cut(s) 157, 916, 979
BstH2I RGCGCY 1 cut(s) 679
BstHHI GCGC 3 cut(s) 593, 678, 900
BstKTI GATC 4 cut(s) 352, 768, 869, 919
BstMAI GTCTC 1 cut(s) 541
BstMBI GATC 4 cut(s) 349, 765, 866, 916
BstMWI GCNNNNNNNGC 8 cut(s) 121, 398, 572, 673, 682, 691, 830, 1048
BstNI CCWGG 2 cut(s) 75, 429
BstNSI RCATGY 1 cut(s) 651
BstSCI CCNGG 2 cut(s) 73, 427
BstSFI CTRYAG 3 cut(s) 668, 1123, 1161
BstV1I GCAGC 4 cut(s) 3, 654, 672, 865
BstV2I GAAGAC 3 cut(s) 269, 461, 860
BstX2I RGATCY 2 cut(s) 866, 916
BstYI RGATCY 2 cut(s) 866, 916
BsuRI GGCC 4 cut(s) 79, 401, 783, 967
BtrI CACGTC 1 cut(s) 995
BtsCI GGATG 3 cut(s) 157, 916, 979
BtsIMutI CAGTG 4 cut(s) 171, 589, 651, 1178
Cac8I GCNNGC 2 cut(s) 81, 526
CaiI CAGNNNCTG 1 cut(s) 869
CciI TCATGA 1 cut(s) 408
CfoI GCGC 3 cut(s) 593, 678, 900
Cfr10I RCCGGY 3 cut(s) 79, 130, 325
Cfr13I GGNCC 2 cut(s) 77, 781
Csp6I GTAC 1 cut(s) 1084
CspAI ACCGGT 1 cut(s) 325
CviAII CATG 5 cut(s) 68, 227, 409, 648, 772
CviQI GTAC 1 cut(s) 1084
DdeI CTNAG 4 cut(s) 54, 632, 681, 785
DpnI GATC 4 cut(s) 351, 767, 868, 918
DpnII GATC 4 cut(s) 349, 765, 866, 916
Eam1104I CTCTTC 4 cut(s) 169, 342, 544, 595
EarI CTCTTC 4 cut(s) 169, 342, 544, 595
Ecl136II GAGCTC 1 cut(s) 179
Eco130I CCWWGG 1 cut(s) 469
Eco24I GRGCYC 1 cut(s) 181
Eco31I GGTCTC 1 cut(s) 541
Eco32I GATATC 2 cut(s) 845, 1158
Eco53kI GAGCTC 1 cut(s) 179
Eco57I CTGAAG 2 cut(s) 323, 1161
Eco88I CYCGRG 1 cut(s) 293
EcoICRI GAGCTC 1 cut(s) 179
EcoRII CCWGG 2 cut(s) 73, 427
EcoRV GATATC 2 cut(s) 845, 1158
EcoT14I CCWWGG 1 cut(s) 469
EcoT38I GRGCYC 1 cut(s) 181
ErhI CCWWGG 1 cut(s) 469
FaeI CATG 5 cut(s) 71, 230, 412, 651, 775
FalI AAGNNNNNCTT 4 cut(s) 501, 533, 551, 583
FaqI GGGAC 2 cut(s) 346, 1016
FatI CATG 5 cut(s) 67, 226, 408, 647, 771
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 4 cut(s) 17, 668, 686, 879
FokI GGATG 3 cut(s) 164, 923, 986
FriOI GRGCYC 1 cut(s) 181
Fsp4HI GCNGC 4 cut(s) 17, 668, 686, 879
FspBI CTAG 1 cut(s) 621
GlaI GCGC 3 cut(s) 592, 677, 899
GluI GCNGC 4 cut(s) 17, 668, 686, 879
GsuI CTGGAG 2 cut(s) 195, 304
HaeII RGCGCY 1 cut(s) 679
HaeIII GGCC 4 cut(s) 79, 401, 783, 967
HapII CCGG 4 cut(s) 80, 101, 131, 326
HhaI GCGC 3 cut(s) 593, 678, 900
Hin1I GRCGYC 2 cut(s) 8, 215
Hin1II CATG 5 cut(s) 71, 230, 412, 651, 775
Hin6I GCGC 3 cut(s) 591, 676, 898
HinP1I GCGC 3 cut(s) 591, 676, 898
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HinfI GANTC 9 cut(s) 92, 110, 242, 254, 291, 299, 334, 545, 617
HpaII CCGG 4 cut(s) 80, 101, 131, 326
HphI GGTGA 2 cut(s) 3, 145
Hpy166II GTNNAC 3 cut(s) 6, 274, 1134
Hpy188I TCNGA 8 cut(s) 208, 506, 544, 715, 816, 871, 921, 954
Hpy188III TCNNGA 6 cut(s) 22, 409, 451, 476, 621, 891
Hpy8I GTNNAC 3 cut(s) 6, 274, 1134
Hpy99I CGWCG 3 cut(s) 10, 217, 220
HpyAV CCTTC 8 cut(s) 19, 40, 142, 187, 412, 503, 1136, 1182
HpyCH4III ACNGT 2 cut(s) 502, 1173
HpyCH4IV ACGT 5 cut(s) 8, 215, 904, 994, 1007
HpyCH4V TGCA 7 cut(s) 575, 651, 670, 688, 759, 824, 881
HpyF10VI GCNNNNNNNGC 8 cut(s) 121, 398, 572, 673, 682, 691, 830, 1048
HpyF3I CTNAG 4 cut(s) 54, 632, 681, 785
HpySE526I ACGT 5 cut(s) 8, 215, 904, 994, 1007
Hsp92I GRCGYC 2 cut(s) 8, 215
Hsp92II CATG 5 cut(s) 71, 230, 412, 651, 775
HspAI GCGC 3 cut(s) 591, 676, 898
KroI GCCGGC 1 cut(s) 79
KroNI GCCGGC 1 cut(s) 81
Kzo9I GATC 4 cut(s) 349, 765, 866, 916
LguI GCTCTTC 1 cut(s) 169
LmnI GCTCC 3 cut(s) 76, 176, 613
Lsp1109I GCAGC 4 cut(s) 3, 654, 672, 865
LweI GCATC 4 cut(s) 111, 142, 377, 798
MaeI CTAG 1 cut(s) 621
MaeII ACGT 5 cut(s) 8, 215, 904, 994, 1007
MaeIII GTNAC 1 cut(s) 9
MalI GATC 4 cut(s) 351, 767, 868, 918
MboI GATC 4 cut(s) 349, 765, 866, 916
MfeI CAATTG 1 cut(s) 594
MflI RGATCY 2 cut(s) 866, 916
MhlI GDGCHC 1 cut(s) 181
MluCI AATT 5 cut(s) 442, 594, 819, 944, 1181
MlyI GAGTC 5 cut(s) 86, 119, 285, 308, 328
MmeI TCCRAC 3 cut(s) 484, 758, 1158
MroNI GCCGGC 1 cut(s) 79
MseI TTAA 2 cut(s) 531, 942
MslI CAYNNNNRTG 2 cut(s) 776, 1101
MspA1I CMGCKG 2 cut(s) 16, 850
MspI CCGG 4 cut(s) 80, 101, 131, 326
MspR9I CCNGG 2 cut(s) 75, 429
MunI CAATTG 1 cut(s) 594
MvaI CCWGG 2 cut(s) 75, 429
MwoI GCNNNNNNNGC 8 cut(s) 121, 398, 572, 673, 682, 691, 830, 1048
NaeI GCCGGC 1 cut(s) 81
NdeII GATC 4 cut(s) 349, 765, 866, 916
NgoMIV GCCGGC 1 cut(s) 79
NlaIII CATG 5 cut(s) 71, 230, 412, 651, 775
NlaIV GGNNCC 2 cut(s) 1000, 1221
NmuCI GTSAC 1 cut(s) 9
NspI RCATGY 1 cut(s) 651
NspV TTCGAA 1 cut(s) 1186
PagI TCATGA 1 cut(s) 408
PciSI GCTCTTC 1 cut(s) 169
PdiI GCCGGC 1 cut(s) 81
PfeI GAWTC 4 cut(s) 242, 254, 545, 617
PinAI ACCGGT 1 cut(s) 325
PkrI GCNGC 4 cut(s) 18, 669, 687, 880
PleI GAGTC 5 cut(s) 86, 118, 285, 307, 328
PpsI GAGTC 5 cut(s) 86, 118, 285, 307, 328
Psp124BI GAGCTC 1 cut(s) 181
Psp1406I AACGTT 1 cut(s) 1007
Psp6I CCWGG 2 cut(s) 73, 427
PspGI CCWGG 2 cut(s) 73, 427
PspN4I GGNNCC 2 cut(s) 1000, 1221
PspPI GGNCC 2 cut(s) 77, 781
PstI CTGCAG 1 cut(s) 672
PstNI CAGNNNCTG 1 cut(s) 869
PsuI RGATCY 2 cut(s) 866, 916
RsaI GTAC 1 cut(s) 1085
RsaNI GTAC 1 cut(s) 1084
RseI CAYNNNNRTG 2 cut(s) 776, 1101
SacI GAGCTC 1 cut(s) 181
SalI GTCGAC 1 cut(s) 4
SapI GCTCTTC 1 cut(s) 169
SaqAI TTAA 2 cut(s) 531, 942
SatI GCNGC 4 cut(s) 17, 668, 686, 879
Sau3AI GATC 4 cut(s) 349, 765, 866, 916
Sau96I GGNCC 2 cut(s) 77, 781
SchI GAGTC 5 cut(s) 86, 119, 285, 308, 328
ScrFI CCNGG 2 cut(s) 75, 429
SduI GDGCHC 1 cut(s) 181
SfaNI GCATC 4 cut(s) 111, 142, 377, 798
SfcI CTRYAG 3 cut(s) 668, 1123, 1161
SfuI TTCGAA 1 cut(s) 1186
SgrAI CRCCGGYG 1 cut(s) 130
SmiMI CAYNNNNRTG 2 cut(s) 776, 1101
SmlI CTYRAG 3 cut(s) 20, 1052, 1058
SmoI CTYRAG 3 cut(s) 20, 1052, 1058
Sse9I AATT 5 cut(s) 442, 594, 819, 944, 1181
SsiI CCGC 4 cut(s) 14, 89, 850, 1129
SspMI CTAG 1 cut(s) 621
SstI GAGCTC 1 cut(s) 181
StyD4I CCNGG 2 cut(s) 73, 427
StyI CCWWGG 1 cut(s) 469
TaaI ACNGT 2 cut(s) 502, 1173
TaiI ACGT 5 cut(s) 11, 218, 907, 997, 1010
TaqI TCGA 6 cut(s) 5, 33, 181, 615, 768, 1186
TaqII GACCGA 1 cut(s) 1004
TasI AATT 5 cut(s) 442, 594, 819, 944, 1181
TfiI GAWTC 4 cut(s) 242, 254, 545, 617
Tru1I TTAA 2 cut(s) 531, 942
Tru9I TTAA 2 cut(s) 531, 942
TscAI CASTG 4 cut(s) 178, 589, 658, 1178
TseFI GTSAC 1 cut(s) 9
TseI GCWGC 4 cut(s) 16, 667, 685, 878
Tsp45I GTSAC 1 cut(s) 9
TspDTI ATGAA 5 cut(s) 223, 425, 788, 927, 1182
TspRI CASTG 4 cut(s) 178, 589, 658, 1178
XapI RAATTY 2 cut(s) 442, 1181
XbaI TCTAGA 1 cut(s) 620
XceI RCATGY 1 cut(s) 651
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 1 cut(s) 621
ZraI GACGTC 2 cut(s) 9, 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.