Rroxscaffold_4G00296310

Alkaline and neutral invertase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0008212
Physical Location & Seq
Reverse (-)
16372369 .. 16376046
3678 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00296310.1

Sequence Viewer

Length: 2007 bp
ATGTCGAAACCGCTCGGAGAATACCTCAAGGAAGAAAGGGATGCAGAGGAGGCGGCCGCTCAGGCTCCGCCTTCTGCTCATGCGACGTCGCCGGAGATAGACTCGCCGAGATCGGATCCATCGATGAAGCTTTCGGATTTCACTGCTGATGACGACTCGGTGTCTTTCGGGCTTCAGCGGCTCAAGGAGATTGCTGAAGCAGCCGAGAAGAAGAAGAAGGCGGAGAAGAATAAGAAGAAGAAGGAGAAGAAGAAGATGAAGAAGAAGCTGCAGGCGGAGAAAGATGTCGGCTCTTCCAAGACTGAAGGTGCGACTCCTAGTGATGAGGATGACTCGACGAAATCGGAAGAAGGTTCTGGAACCTGTGAGAAGAGCGAGAGTGCCGAATTGGCCAAGAAAGATCCATTGTCGCCGGAGAAGTGTACGGGAACGTCCGCGACGCCGCCGTCCACAGGGAAGGCAAAGAAGTCGGTGAAACTGAAACTGCAGAGACCCGACGAAGCTCCGAAGCAGACGACGTCGCTGCTGATGTCGTATCAAAGTATGAGGAGTCCTGAGTTGAGTGCACTCATCGTGGAATCTTCGAAGGAATCTTCGGCGGCTATGGTGGAGGAGGCCTGGGAGAGGCTCAAGAAGTCGTACGTTTACTACAGAGGCAAGCCGGTGGGCACTCTTGCTGCTATGGATCCAACGGCCGAGGCTCTAAACTACAATCAGGTATTTGTGAGGGATTTTGTTCCTACTGGTTTGGCCTGCCTCATGAAGAATCCAGCAGAGCCAGAGATAGTAAAGAACTTTCTCCTAAAGACCCTCCACCTCCAAGGTCGAGAGAAAATGGTTGATAACTTTACACTTGGAGAAGGTGTTCTCCCTGCGAGCTTTAAGGTTTCAAATGATTCAGATCGTGCCAAGGAAACTATAGTTGCAGATTTTGGTGGTAGCGCAATTGGAAGAGTTGCTCCGGTTGATTCTGGATTCTGGTGGATCATATTGCTGTGGTCATATAACAGATGCACTCGTGATCATGTGCTGGTGGAGCTCCCTGCGGTGCAAAATGGAATGAAACTAATACTCAACCTCTGCCTTTCAGATGGCTTTGACACATTTCCAACACTTCTTTGTGCAGATGGCTGCAGTATGATTGACAGGAGGATGGGCATATATGGTTATCCAATTGAGATCCAAGCACTTTTCTACTTTGCATTGAGATGTGCAATGCAGCTGTTGAAGCCAGAGCGTGGTGGTAAGGAACTTCTTGAGAGGATAGATAAGCGCATTATAGCTCTCAGCTTTCACATCCAGAAGTACTACTGGCTTGACTTTACTCATCTCAATAACATATACCGCTACAAAACGGAGGAGTATTCCCATACAGCTGTCAACAAGTTCAATGTGATGCCAGAGTCCATCCCTGATTGGGTTTTTGATTTCATGCCATTGCGAGGAGGGTATTTGATTGGCAATGTTAGCCCAGCTCGCATGGATTTCCGGTGGTTCTTAGTTGGGAACTGCATTGCAATTCTTAGCTGTTTAGCCACACCTGATCAAGCTACAGCCATAATGGATCTGATTGAGGAGCGTTGGGAGGATTTGATAGGTGAGATGCCTCTGAAAATTGCATATCCAGCATTAGAGGGACATGAGTGGAGGACAATCACAGGATATGACCCCAAAAATACACGATGGAGCTACCACAATGGCGGTTCATGGCCAACACTGCTGTGGCTGCTGGCTGCGGCTTGCATCAAGACTGGGAGGCCTCAGATTGCAAAGCGGGCAATAGAGCTGGCAGAGCAGCGGCTCTCCAAAGACGGGTGGCCTGAGTACTATGATGGTAAAACAGGGAGATACATTGGGAAGCAAGCGAGGAGGTACCAGACATGGAGCATCTCTGGCTATCTGATTGCTAAGATGATGACAGAGAATCCATCCAATCTTCTATTGGTCTCTCTCGAGGAGGACAAGAAAATTGCCAAGCCAAGGCTCACCCGTTCTGCTACGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_100

Protein Analysis

668

Amino Acids

75.04

Weight (kDa)

8.25

Isoelectric Point (pI)

48.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_100 PF12899 206 - 643 8.2e-212 Alkaline and neutral invertase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 445
AatII GACGTC 2 cut(s) 89, 521
Acc65I GGTACC 1 cut(s) 1872
AccB1I GGYRCC 1 cut(s) 1872
AccB7I CCANNNNNTGG 1 cut(s) 1238
AccBSI CCGCTC 2 cut(s) 13, 59
AccII CGCG 1 cut(s) 437
AclWI GGATC 8 cut(s) 110, 123, 395, 680, 693, 992, 1174, 1572
AcoI YGGCCR 4 cut(s) 54, 390, 693, 1709
AcuI CTGAAG 3 cut(s) 158, 216, 324
AcyI GRCGYC 3 cut(s) 86, 440, 518
AfaI GTAC 5 cut(s) 424, 641, 1307, 1826, 1874
AfiI CCNNNNNNNGG 7 cut(s) 452, 624, 1238, 1417, 1442, 1812, 1980
AgsI TTSAA 3 cut(s) 891, 1228, 1390
AhdI GACNNNNNGTC 1 cut(s) 160
AjnI CCWGG 1 cut(s) 617
AleI CACNNNNGTG 1 cut(s) 1720
AloI GAACNNNNNNTCC 2 cut(s) 849, 881
Alw21I GWGCWC 2 cut(s) 568, 1041
Alw26I GTCTC 2 cut(s) 484, 1951
Alw44I GTGCAC 1 cut(s) 564
AlwI GGATC 8 cut(s) 110, 123, 395, 680, 693, 992, 1174, 1572
Ama87I CYCGRG 1 cut(s) 1952
AoxI GGCC 8 cut(s) 54, 390, 615, 693, 750, 1709, 1757, 1817
ApaLI GTGCAC 1 cut(s) 564
ApeKI GCWGC 9 cut(s) 200, 268, 523, 677, 1131, 1219, 1726, 1733, 1795
Asp700I GAANNNNTTC 1 cut(s) 864
Asp718I GGTACC 1 cut(s) 1872
AspLEI GCGC 2 cut(s) 944, 1275
AsuHPI GGTGA 3 cut(s) 484, 1610, 1978
AsuII TTCGAA 1 cut(s) 584
AvaI CYCGRG 1 cut(s) 1952
BaeGI GKGCMC 2 cut(s) 568, 671
BalI TGGCCA 2 cut(s) 392, 1711
BamHI GGATCC 2 cut(s) 115, 685
BanI GGYRCC 1 cut(s) 1872
BanII GRGCYC 1 cut(s) 1041
BauI CACGAG 1 cut(s) 1017
Bbv12I GWGCWC 2 cut(s) 568, 1041
BbvI GCAGC 9 cut(s) 212, 255, 510, 664, 1118, 1231, 1713, 1720, 1807
BccI CCATC 8 cut(s) 127, 1085, 1121, 1147, 1415, 1677, 1826, 1936
BceAI ACGGC 2 cut(s) 430, 708
BcgI CGANNNNNNTGC 4 cut(s) 450, 484, 505, 539
BciT130I CCWGG 1 cut(s) 619
BclI TGATCA 2 cut(s) 1021, 1543
BcoDI GTCTC 2 cut(s) 484, 1951
BfaI CTAG 1 cut(s) 318
BfmI CTRYAG 6 cut(s) 269, 485, 649, 918, 1132, 1551
BglI GCCNNNNNGGC 2 cut(s) 62, 389
BmcAI AGTACT 2 cut(s) 1307, 1826
Bme1390I CCNGG 1 cut(s) 619
BmeRI GACNNNNNGTC 1 cut(s) 160
BmeT110I CYCGRG 1 cut(s) 1952
BmiI GGNNCC 5 cut(s) 66, 117, 361, 687, 1874
BmrFI CCNGG 1 cut(s) 619
BmrI ACTGGG 1 cut(s) 1761
BmsI GCATC 6 cut(s) 31, 1001, 1386, 1593, 1752, 1896
BmuI ACTGGG 1 cut(s) 1761
BplI GAGNNNNNCTC 6 cut(s) 9, 41, 86, 118, 317, 349
Bpu10I CCTNAGC 1 cut(s) 60
Bpu14I TTCGAA 1 cut(s) 584
BpuEI CTTGAG 4 cut(s) 11, 167, 614, 1277
Bsa29I ATCGAT 1 cut(s) 122
BsaBI GATNNNNATC 1 cut(s) 900
BsaHI GRCGYC 3 cut(s) 86, 440, 518
BsaI GGTCTC 2 cut(s) 484, 1951
BsaJI CCNNGG 5 cut(s) 618, 696, 820, 909, 1979
BsaWI WCCGGW 2 cut(s) 961, 1488
BsaXI ACNNNNNCTCC 2 cut(s) 849, 879
Bsc4I CCNNNNNNNGG 7 cut(s) 452, 624, 1238, 1417, 1442, 1812, 1980
Bse118I RCCGGY 1 cut(s) 661
Bse1I ACTGG 3 cut(s) 748, 1316, 1756
Bse3DI GCAATG 4 cut(s) 1221, 1436, 1468, 1512
Bse8I GATNNNNATC 1 cut(s) 900
BseBI CCWGG 1 cut(s) 619
BseCI ATCGAT 1 cut(s) 122
BseDI CCNNGG 5 cut(s) 618, 696, 820, 909, 1979
BseGI GGATG 6 cut(s) 46, 334, 1158, 1296, 1407, 1928
BseJI GATNNNNATC 1 cut(s) 900
BseLI CCNNNNNNNGG 7 cut(s) 452, 624, 1238, 1417, 1442, 1812, 1980
BseMI GCAATG 4 cut(s) 1221, 1436, 1468, 1512
BseMII CTCAG 5 cut(s) 74, 546, 1300, 1775, 1812
BseNI ACTGG 3 cut(s) 748, 1316, 1756
BseRI GAGGAG 8 cut(s) 62, 562, 626, 1373, 1458, 1589, 1882, 1970
BseSI GKGCMC 2 cut(s) 568, 671
BseX3I CGGCCG 2 cut(s) 54, 693
BseXI GCAGC 9 cut(s) 212, 255, 510, 664, 1118, 1231, 1713, 1720, 1807
BseYI CCCAGC 1 cut(s) 1471
BsgI GTGCAG 1 cut(s) 1143
Bsh1236I CGCG 1 cut(s) 437
Bsh1285I CGRYCG 2 cut(s) 57, 696
BshFI GGCC 8 cut(s) 56, 392, 617, 695, 752, 1711, 1759, 1819
BshNI GGYRCC 1 cut(s) 1872
BshVI ATCGAT 1 cut(s) 122
BsiEI CGRYCG 2 cut(s) 57, 696
BsiHKAI GWGCWC 2 cut(s) 568, 1041
BsiHKCI CYCGRG 1 cut(s) 1952
BsiSI CCGG 5 cut(s) 92, 413, 662, 962, 1489
BsiWI CGTACG 1 cut(s) 639
BslFI GGGAC 1 cut(s) 1650
BslI CCNNNNNNNGG 7 cut(s) 452, 624, 1238, 1417, 1442, 1812, 1980
BsmAI GTCTC 2 cut(s) 484, 1951
BsmFI GGGAC 1 cut(s) 1650
BsnI GGCC 8 cut(s) 56, 392, 617, 695, 752, 1711, 1759, 1819
Bso31I GGTCTC 2 cut(s) 484, 1951
BsoBI CYCGRG 1 cut(s) 1952
Bsp119I TTCGAA 1 cut(s) 584
Bsp1286I GDGCHC 3 cut(s) 568, 671, 1041
BspANI GGCC 8 cut(s) 56, 392, 617, 695, 752, 1711, 1759, 1819
BspCNI CTCAG 5 cut(s) 73, 547, 1299, 1774, 1813
BspDI ATCGAT 1 cut(s) 122
BspFNI CGCG 1 cut(s) 437
BspHI TCATGA 1 cut(s) 759
BspLI GGNNCC 5 cut(s) 66, 117, 361, 687, 1874
BspMAI CTGCAG 3 cut(s) 273, 489, 1136
BspPI GGATC 8 cut(s) 110, 123, 395, 680, 693, 992, 1174, 1572
BspQI GCTCTTC 2 cut(s) 298, 365
BspT104I TTCGAA 1 cut(s) 584
BspT107I GGYRCC 1 cut(s) 1872
BspTNI GGTCTC 2 cut(s) 484, 1951
BsrBI CCGCTC 2 cut(s) 13, 59
BsrDI GCAATG 4 cut(s) 1221, 1436, 1468, 1512
BsrFI RCCGGY 1 cut(s) 661
BsrI ACTGG 3 cut(s) 748, 1316, 1756
BssAI RCCGGY 1 cut(s) 661
BssECI CCNNGG 5 cut(s) 618, 696, 820, 909, 1979
BssNI GRCGYC 3 cut(s) 86, 440, 518
BssSI CACGAG 1 cut(s) 1017
BssT1I CCWWGG 3 cut(s) 820, 909, 1979
Bst2BI CACGAG 1 cut(s) 1017
Bst2UI CCWGG 1 cut(s) 619
Bst4CI ACNGT 1 cut(s) 2002
Bst6I CTCTTC 3 cut(s) 298, 365, 946
BstACI GRCGYC 3 cut(s) 86, 440, 518
BstBI TTCGAA 1 cut(s) 584
BstDEI CTNAG 8 cut(s) 60, 555, 1286, 1498, 1523, 1761, 1821, 1908
BstENI CCTNNNNNAGG 1 cut(s) 622
BstF5I GGATG 6 cut(s) 46, 334, 1158, 1296, 1407, 1928
BstFNI CGCG 1 cut(s) 437
BstHHI GCGC 2 cut(s) 944, 1275
BstMAI GTCTC 2 cut(s) 484, 1951
BstMCI CGRYCG 2 cut(s) 57, 696
BstNI CCWGG 1 cut(s) 619
BstSCI CCNGG 1 cut(s) 617
BstSFI CTRYAG 6 cut(s) 269, 485, 649, 918, 1132, 1551
BstSLI GKGCMC 2 cut(s) 568, 671
BstUI CGCG 1 cut(s) 437
BstV1I GCAGC 9 cut(s) 212, 255, 510, 664, 1118, 1231, 1713, 1720, 1807
BstX2I RGATCY 5 cut(s) 115, 400, 685, 1179, 1564
BstYI RGATCY 5 cut(s) 115, 400, 685, 1179, 1564
BstZI CGGCCG 2 cut(s) 54, 693
Bsu15I ATCGAT 1 cut(s) 122
BsuRI GGCC 8 cut(s) 56, 392, 617, 695, 752, 1711, 1759, 1819
BsuTUI ATCGAT 1 cut(s) 122
BtsCI GGATG 6 cut(s) 46, 334, 1158, 1296, 1407, 1928
BtsI GCAGTG 2 cut(s) 141, 1715
BtsIMutI CAGTG 2 cut(s) 141, 1715
CciI TCATGA 1 cut(s) 759
CciNI GCGGCCGC 1 cut(s) 54
CfoI GCGC 2 cut(s) 944, 1275
Cfr10I RCCGGY 1 cut(s) 661
ClaI ATCGAT 1 cut(s) 122
CseI GACGC 1 cut(s) 448
Csp6I GTAC 5 cut(s) 423, 640, 1306, 1825, 1873
CviAII CATG 8 cut(s) 80, 760, 1025, 1432, 1480, 1640, 1707, 1881
CviQI GTAC 5 cut(s) 423, 640, 1306, 1825, 1873
DdeI CTNAG 8 cut(s) 60, 555, 1286, 1498, 1523, 1761, 1821, 1908
DrdI GACNNNNNNGTC 1 cut(s) 445
DriI GACNNNNNGTC 1 cut(s) 160
DseDI GACNNNNNNGTC 1 cut(s) 445
EaeI YGGCCR 4 cut(s) 54, 390, 693, 1709
EagI CGGCCG 2 cut(s) 54, 693
Eam1104I CTCTTC 3 cut(s) 298, 365, 946
Eam1105I GACNNNNNGTC 1 cut(s) 160
EarI CTCTTC 3 cut(s) 298, 365, 946
EciI GGCGGA 3 cut(s) 57, 236, 290
Ecl136II GAGCTC 1 cut(s) 1039
EclXI CGGCCG 2 cut(s) 54, 693
Eco130I CCWWGG 3 cut(s) 820, 909, 1979
Eco147I AGGCCT 2 cut(s) 617, 1759
Eco24I GRGCYC 1 cut(s) 1041
Eco31I GGTCTC 2 cut(s) 484, 1951
Eco52I CGGCCG 2 cut(s) 54, 693
Eco53kI GAGCTC 1 cut(s) 1039
Eco57I CTGAAG 3 cut(s) 158, 216, 324
Eco88I CYCGRG 1 cut(s) 1952
EcoICRI GAGCTC 1 cut(s) 1039
EcoNI CCTNNNNNAGG 1 cut(s) 622
EcoRII CCWGG 1 cut(s) 617
EcoT14I CCWWGG 3 cut(s) 820, 909, 1979
EcoT38I GRGCYC 1 cut(s) 1041
ErhI CCWWGG 3 cut(s) 820, 909, 1979
FaeI CATG 8 cut(s) 83, 763, 1028, 1435, 1483, 1643, 1710, 1884
FaqI GGGAC 1 cut(s) 1650
FatI CATG 8 cut(s) 79, 759, 1024, 1431, 1479, 1639, 1706, 1880
FauI CCCGC 1 cut(s) 1767
FbaI TGATCA 2 cut(s) 1021, 1543
FokI GGATG 6 cut(s) 53, 341, 1165, 1283, 1394, 1915
FriOI GRGCYC 1 cut(s) 1041
FspBI CTAG 1 cut(s) 318
GlaI GCGC 2 cut(s) 943, 1274
GsaI CCCAGC 1 cut(s) 1475
HaeIII GGCC 8 cut(s) 56, 392, 617, 695, 752, 1711, 1759, 1819
HapII CCGG 5 cut(s) 92, 413, 662, 962, 1489
HgaI GACGC 1 cut(s) 448
HhaI GCGC 2 cut(s) 944, 1275
Hin1I GRCGYC 3 cut(s) 86, 440, 518
Hin1II CATG 8 cut(s) 83, 763, 1028, 1435, 1483, 1643, 1710, 1884
Hin6I GCGC 2 cut(s) 942, 1273
HinP1I GCGC 2 cut(s) 942, 1273
HincII GTYRAC 1 cut(s) 1381
HindII GTYRAC 1 cut(s) 1381
HindIII AAGCTT 1 cut(s) 128
HpaII CCGG 5 cut(s) 92, 413, 662, 962, 1489
HphI GGTGA 3 cut(s) 484, 1610, 1978
Hpy166II GTNNAC 5 cut(s) 423, 450, 566, 646, 1381
Hpy8I GTNNAC 5 cut(s) 423, 450, 566, 646, 1381
Hpy99I CGWCG 7 cut(s) 88, 91, 340, 442, 500, 520, 523
HpyAV CCTTC 8 cut(s) 81, 211, 235, 299, 344, 451, 580, 854
HpyCH4III ACNGT 1 cut(s) 2002
HpyCH4IV ACGT 4 cut(s) 86, 431, 518, 642
HpyF3I CTNAG 8 cut(s) 60, 555, 1286, 1498, 1523, 1761, 1821, 1908
HpySE526I ACGT 4 cut(s) 86, 431, 518, 642
Hsp92I GRCGYC 3 cut(s) 86, 440, 518
Hsp92II CATG 8 cut(s) 83, 763, 1028, 1435, 1483, 1643, 1710, 1884
HspAI GCGC 2 cut(s) 942, 1273
KpnI GGTACC 1 cut(s) 1876
Ksp22I TGATCA 2 cut(s) 1021, 1543
LguI GCTCTTC 2 cut(s) 298, 365
LmnI GCTCC 8 cut(s) 70, 508, 964, 1036, 1044, 1576, 1686, 1884
Lsp1109I GCAGC 9 cut(s) 212, 255, 510, 664, 1118, 1231, 1713, 1720, 1807
LweI GCATC 6 cut(s) 31, 1001, 1386, 1593, 1752, 1896
MaeI CTAG 1 cut(s) 318
MaeII ACGT 4 cut(s) 86, 431, 518, 642
MbiI CCGCTC 2 cut(s) 13, 59
MfeI CAATTG 2 cut(s) 945, 1173
MflI RGATCY 5 cut(s) 115, 400, 685, 1179, 1564
MhlI GDGCHC 3 cut(s) 568, 671, 1041
MlsI TGGCCA 2 cut(s) 392, 1711
MluCI AATT 6 cut(s) 386, 945, 1173, 1518, 1614, 1968
MluNI TGGCCA 2 cut(s) 392, 1711
MlyI GAGTC 6 cut(s) 95, 149, 307, 326, 559, 1412
MmeI TCCRAC 2 cut(s) 713, 1133
Mox20I TGGCCA 2 cut(s) 392, 1711
MroXI GAANNNNTTC 1 cut(s) 864
MscI TGGCCA 2 cut(s) 392, 1711
MseI TTAA 2 cut(s) 882, 2005
MslI CAYNNNNRTG 2 cut(s) 1207, 1720
Msp20I TGGCCA 2 cut(s) 392, 1711
MspA1I CMGCKG 4 cut(s) 178, 1222, 1376, 1798
MspI CCGG 5 cut(s) 92, 413, 662, 962, 1489
MspR9I CCNGG 1 cut(s) 619
MunI CAATTG 2 cut(s) 945, 1173
MvaI CCWGG 1 cut(s) 619
MvnI CGCG 1 cut(s) 437
NlaIII CATG 8 cut(s) 83, 763, 1028, 1435, 1483, 1643, 1710, 1884
NlaIV GGNNCC 5 cut(s) 66, 117, 361, 687, 1874
NmeAIII GCCGAG 3 cut(s) 132, 229, 721
NotI GCGGCCGC 1 cut(s) 54
NspV TTCGAA 1 cut(s) 584
OliI CACNNNNGTG 1 cut(s) 1720
PaeR7I CTCGAG 1 cut(s) 1952
PagI TCATGA 1 cut(s) 759
PceI AGGCCT 2 cut(s) 617, 1759
PciSI GCTCTTC 2 cut(s) 298, 365
PcsI WCGNNNNNNNCGW 1 cut(s) 119
PdmI GAANNNNTTC 1 cut(s) 864
PfeI GAWTC 7 cut(s) 578, 590, 766, 896, 968, 975, 1924
Pfl23II CGTACG 1 cut(s) 639
PflFI GACNNNGTC 1 cut(s) 517
PflMI CCANNNNNTGG 1 cut(s) 1238
PleI GAGTC 6 cut(s) 95, 149, 307, 326, 558, 1411
PpsI GAGTC 6 cut(s) 95, 149, 307, 326, 558, 1411
Psp124BI GAGCTC 1 cut(s) 1041
Psp6I CCWGG 1 cut(s) 617
PspFI CCCAGC 1 cut(s) 1471
PspGI CCWGG 1 cut(s) 617
PspLI CGTACG 1 cut(s) 639
PspN4I GGNNCC 5 cut(s) 66, 117, 361, 687, 1874
PstI CTGCAG 3 cut(s) 273, 489, 1136
PsuI RGATCY 5 cut(s) 115, 400, 685, 1179, 1564
PsyI GACNNNGTC 1 cut(s) 517
PvuII CAGCTG 2 cut(s) 1222, 1376
RsaI GTAC 5 cut(s) 424, 641, 1307, 1826, 1874
RsaNI GTAC 5 cut(s) 423, 640, 1306, 1825, 1873
RseI CAYNNNNRTG 2 cut(s) 1207, 1720
SacI GAGCTC 1 cut(s) 1041
SapI GCTCTTC 2 cut(s) 298, 365
SaqAI TTAA 2 cut(s) 882, 2005
ScaI AGTACT 2 cut(s) 1307, 1826
SchI GAGTC 6 cut(s) 95, 149, 307, 326, 559, 1412
ScrFI CCNGG 1 cut(s) 619
SduI GDGCHC 3 cut(s) 568, 671, 1041
SfaNI GCATC 6 cut(s) 31, 1001, 1386, 1593, 1752, 1896
SfcI CTRYAG 6 cut(s) 269, 485, 649, 918, 1132, 1551
Sfr274I CTCGAG 1 cut(s) 1952
SfuI TTCGAA 1 cut(s) 584
SlaI CTCGAG 1 cut(s) 1952
SmiMI CAYNNNNRTG 2 cut(s) 1207, 1720
SmlI CTYRAG 5 cut(s) 26, 182, 629, 1256, 1952
SmoI CTYRAG 5 cut(s) 26, 182, 629, 1256, 1952
Sse9I AATT 6 cut(s) 386, 945, 1173, 1518, 1614, 1968
SseBI AGGCCT 2 cut(s) 617, 1759
SspMI CTAG 1 cut(s) 318
SstI GAGCTC 1 cut(s) 1041
StuI AGGCCT 2 cut(s) 617, 1759
StyD4I CCNGG 1 cut(s) 617
StyI CCWWGG 3 cut(s) 820, 909, 1979
TaaI ACNGT 1 cut(s) 2002
TaiI ACGT 4 cut(s) 89, 434, 521, 645
TaqI TCGA 6 cut(s) 5, 122, 335, 584, 826, 1953
TasI AATT 6 cut(s) 386, 945, 1173, 1518, 1614, 1968
TatI WGTACW 2 cut(s) 1305, 1824
TauI GCSGC 7 cut(s) 56, 59, 181, 445, 602, 1739, 1801
TfiI GAWTC 7 cut(s) 578, 590, 766, 896, 968, 975, 1924
Tru1I TTAA 2 cut(s) 882, 2005
Tru9I TTAA 2 cut(s) 882, 2005
TscAI CASTG 2 cut(s) 148, 1722
TseI GCWGC 9 cut(s) 200, 268, 523, 677, 1131, 1219, 1726, 1733, 1795
TspDTI ATGAA 6 cut(s) 140, 272, 776, 1076, 1420, 1695
TspGWI ACGGA 1 cut(s) 1370
TspRI CASTG 2 cut(s) 148, 1722
Tth111I GACNNNGTC 1 cut(s) 517
Van91I CCANNNNNTGG 1 cut(s) 1238
VneI GTGCAC 1 cut(s) 564
XagI CCTNNNNNAGG 1 cut(s) 622
XcmI CCANNNNNNNNNTGG 2 cut(s) 1719, 1939
XhoI CTCGAG 1 cut(s) 1952
XmnI GAANNNNTTC 1 cut(s) 864
XspI CTAG 1 cut(s) 318
ZraI GACGTC 2 cut(s) 87, 519
ZrmI AGTACT 2 cut(s) 1307, 1826
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.