RLG00000027393

Alkaline and neutral invertase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0008212
Physical Location & Seq
Forward (+)
11201211 .. 11203340
2130 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027393

Sequence Viewer

Length: 1320 bp
ATGAGGAGTCCTGAGTTGAGTGCACTCATCGTGGAATCTTTGATGGAATCTTCGGCGGCTATGGTGGAGGAGACCTGGGAGAGACTCAAGAGGTCGTTCGTTTACTACAGAGGCAAGCCGGTGGGGACTGTTTCTGCTACGGATCCAATTGCCGAGGCTGTATTTGTGAGAGATTTTATCCCTACTGGTTTGGCCTGCCTCATGAACGTTCCAGCAGAGCCAGAGATAGTTCTGAACTTTCTCCTAGAGACCCTCCTCCATCTCCAAGGTCCAGAGAAAATGGCTGATATCTTAACACTTGGAGAAGGTATTCTTCCTGCGAGCTTTAAGGTTTCAAATGATTCAGATCGTGCCAAGGAAACTATCGTTGCAGATTTTGGTAGTACCGCAATTGGAAGAGTTGCTCCGGTTGATTCTGGATTCTGTTGGATCACATATGGCATATGTACTACTCATGATCATGCGCTGGTGGAGCTCCCTGTGGTGCAAAATGGAATGAGAGTTCTCAACCTCTGCCCTTCAGATGGCTTTGACACATTTCCAACAGTACTTTGTGCAGATGGCTGCAGTATGACTGACAGCAGGATGTTCATATATGGTTATCCAGTTGAGATCCAAGCACTTTTCTACTTTGCATTGAGGTGGGCTATGCAGTTGTTGAAGCCAGGGTGGGAAGCAAAGGAACTTCTTGAGAGTATGGATAAGAGCATCACAGCTCTCAGTTTTCCAAAGTCCTCCAATGCAGCTATTGAGAAGCTTAATGCTGTCCGGCTGGACTACTCCATCCCTGATTGGGTTTTTGATTTTATGCCATTGCGAGGAGGAGGCTGGAGTAAAAAGGTTGTTCATTACGCTGTAGGCACATCAAACCCTGCTCGAGCTACAGATACCTTCCCAGCAGCTAGTTCATCAGCTGGGGCTCACAAATGCCCTGCAGCTGGTCCATCAACCGGTGCTCAGAAGCGCAAAAGAGAAGATGATGTCAGATCTACTGGACCATCTAAAAGGATCCTCCTGATGATGGAGCACATTGATGTGTACTGGATCGATAACGATGAAAACGAACATTTTGTTGGAATAGTAGAGGGTGTATACAGCATGGTGAGCCCGCTGGAGCTTGAGATGGCTGTTACCCATGTGTTAGTGCATCACCAATTACCTGCAGAGAACACCAAAGTTTATTACAGTATATGGACTGACCAAGTGCTGGTTGAGCTACCAATATTAACAACGAATTGGGATTATTTCAAGGACCGGGTGAGGGGGGTTCAGGTGAGGGCTTTTGAGGACGAAGAAGAAGAAGAAGAAGAAGAGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_100

Protein Analysis

440

Amino Acids

48.91

Weight (kDa)

4.78

Isoelectric Point (pI)

45.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_100 PF12899 26 - 242 9.1e-59 Alkaline and neutral invertase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1168
AccB7I CCANNNNNTGG 1 cut(s) 1207
AccI GTMKAC 1 cut(s) 1092
AciI CCGC 3 cut(s) 56, 387, 1109
AclI AACGTT 1 cut(s) 207
AclWI GGATC 7 cut(s) 137, 150, 437, 607, 1003, 1016, 1052
AcuI CTGAAG 1 cut(s) 504
AfaI GTAC 4 cut(s) 385, 448, 549, 1040
AfiI CCNNNNNNNGG 8 cut(s) 524, 793, 818, 938, 950, 1021, 1207, 1261
AgeI ACCGGT 1 cut(s) 950
AgsI TTSAA 3 cut(s) 336, 661, 1249
AjnI CCWGG 2 cut(s) 74, 664
AjuI GAANNNNNNNTTGG 2 cut(s) 1056, 1088
AloI GAACNNNNNNTCC 2 cut(s) 486, 518
Alw21I GWGCWC 4 cut(s) 25, 477, 958, 1029
Alw26I GTCTC 3 cut(s) 65, 76, 242
Alw44I GTGCAC 1 cut(s) 21
AlwI GGATC 7 cut(s) 137, 150, 437, 607, 1003, 1016, 1052
Ama87I CYCGRG 1 cut(s) 876
AoxI GGCC 1 cut(s) 192
ApaLI GTGCAC 1 cut(s) 21
ApeKI GCWGC 4 cut(s) 564, 743, 899, 935
AsiGI ACCGGT 1 cut(s) 950
Asp700I GAANNNNTTC 1 cut(s) 309
AspLEI GCGC 2 cut(s) 466, 966
AspS9I GGNCC 4 cut(s) 269, 941, 995, 1252
AsuC2I CCSGG 1 cut(s) 1256
AsuHPI GGTGA 4 cut(s) 1114, 1142, 1270, 1285
AvaI CYCGRG 1 cut(s) 876
AvaII GGWCC 4 cut(s) 269, 941, 995, 1252
BaeGI GKGCMC 1 cut(s) 25
BamHI GGATCC 2 cut(s) 142, 1008
BanII GRGCYC 3 cut(s) 477, 922, 1109
Bbv12I GWGCWC 4 cut(s) 25, 477, 958, 1029
BbvI GCAGC 4 cut(s) 551, 755, 911, 947
BccI CCATC 9 cut(s) 37, 267, 518, 554, 791, 952, 1006, 1015, 1117
BciT130I CCWGG 2 cut(s) 76, 666
BclI TGATCA 1 cut(s) 457
BcnI CCSGG 1 cut(s) 1256
BcoDI GTCTC 3 cut(s) 65, 76, 242
BfaI CTAG 2 cut(s) 245, 903
BfmI CTRYAG 6 cut(s) 106, 565, 855, 882, 933, 1161
BfuAI ACCTGC 1 cut(s) 1168
BglII AGATCT 1 cut(s) 986
BisI GCNGC 5 cut(s) 57, 565, 744, 900, 936
BlsI GCNGC 5 cut(s) 58, 566, 745, 901, 937
BmcAI AGTACT 1 cut(s) 549
Bme1390I CCNGG 3 cut(s) 76, 666, 1256
Bme18I GGWCC 4 cut(s) 269, 941, 995, 1252
BmeT110I CYCGRG 1 cut(s) 876
BmgT120I GGNCC 4 cut(s) 269, 941, 995, 1252
BmiI GGNNCC 2 cut(s) 144, 1010
BmrFI CCNGG 3 cut(s) 76, 666, 1256
BmsI GCATC 2 cut(s) 717, 1156
BpmI CTGGAG 2 cut(s) 850, 1133
BpuEI CTTGAG 3 cut(s) 71, 710, 1139
BpuMI CCSGG 1 cut(s) 1256
Bsa29I ATCGAT 1 cut(s) 1047
BsaBI GATNNNNATC 1 cut(s) 345
BsaI GGTCTC 2 cut(s) 65, 242
BsaJI CCNNGG 5 cut(s) 75, 153, 265, 354, 665
BsaWI WCCGGW 2 cut(s) 406, 950
Bsc4I CCNNNNNNNGG 8 cut(s) 524, 793, 818, 938, 950, 1021, 1207, 1261
Bse118I RCCGGY 2 cut(s) 118, 950
Bse1I ACTGG 4 cut(s) 190, 605, 997, 1046
Bse3DI GCAATG 1 cut(s) 812
Bse8I GATNNNNATC 1 cut(s) 345
BseBI CCWGG 2 cut(s) 76, 666
BseCI ATCGAT 1 cut(s) 1047
BseDI CCNNGG 5 cut(s) 75, 153, 265, 354, 665
BseGI GGATG 2 cut(s) 591, 783
BseJI GATNNNNATC 1 cut(s) 345
BseLI CCNNNNNNNGG 8 cut(s) 524, 793, 818, 938, 950, 1021, 1207, 1261
BseMI GCAATG 1 cut(s) 812
BseMII CTCAG 2 cut(s) 733, 971
BseNI ACTGG 4 cut(s) 190, 605, 997, 1046
BseRI GAGGAG 5 cut(s) 19, 83, 245, 834, 837
BseSI GKGCMC 1 cut(s) 25
BseXI GCAGC 4 cut(s) 551, 755, 911, 947
BseYI CCCAGC 2 cut(s) 895, 914
BsgI GTGCAG 1 cut(s) 576
BshFI GGCC 1 cut(s) 194
BshTI ACCGGT 1 cut(s) 950
BshVI ATCGAT 1 cut(s) 1047
BsiHKAI GWGCWC 4 cut(s) 25, 477, 958, 1029
BsiHKCI CYCGRG 1 cut(s) 876
BsiSI CCGG 5 cut(s) 119, 407, 769, 951, 1255
BslFI GGGAC 1 cut(s) 139
BslI CCNNNNNNNGG 8 cut(s) 524, 793, 818, 938, 950, 1021, 1207, 1261
BsmAI GTCTC 3 cut(s) 65, 76, 242
BsmFI GGGAC 1 cut(s) 139
BsnI GGCC 1 cut(s) 194
Bso31I GGTCTC 2 cut(s) 65, 242
BsoBI CYCGRG 1 cut(s) 876
Bsp1286I GDGCHC 6 cut(s) 25, 477, 922, 958, 1029, 1109
Bsp143I GATC 8 cut(s) 142, 346, 429, 457, 612, 986, 1008, 1044
BspACI CCGC 3 cut(s) 56, 387, 1109
BspANI GGCC 1 cut(s) 194
BspCNI CTCAG 3 cut(s) 4, 732, 970
BspDI ATCGAT 1 cut(s) 1047
BspHI TCATGA 2 cut(s) 201, 454
BspLI GGNNCC 2 cut(s) 144, 1010
BspMAI CTGCAG 3 cut(s) 569, 937, 1165
BspMI ACCTGC 1 cut(s) 1168
BspPI GGATC 7 cut(s) 137, 150, 437, 607, 1003, 1016, 1052
BspTNI GGTCTC 2 cut(s) 65, 242
BsrDI GCAATG 1 cut(s) 812
BsrFI RCCGGY 2 cut(s) 118, 950
BsrI ACTGG 4 cut(s) 190, 605, 997, 1046
BssAI RCCGGY 2 cut(s) 118, 950
BssECI CCNNGG 5 cut(s) 75, 153, 265, 354, 665
BssMI GATC 8 cut(s) 142, 346, 429, 457, 612, 986, 1008, 1044
BssNAI GTATAC 1 cut(s) 1093
BssT1I CCWWGG 2 cut(s) 265, 354
Bst1107I GTATAC 1 cut(s) 1093
Bst2UI CCWGG 2 cut(s) 76, 666
Bst4CI ACNGT 3 cut(s) 130, 547, 1187
Bst6I CTCTTC 2 cut(s) 391, 1305
BstC8I GCNNGC 4 cut(s) 116, 196, 322, 1109
BstDEI CTNAG 3 cut(s) 12, 719, 957
BstF5I GGATG 2 cut(s) 591, 783
BstHHI GCGC 2 cut(s) 466, 966
BstKTI GATC 8 cut(s) 145, 349, 432, 460, 615, 989, 1011, 1047
BstMAI GTCTC 3 cut(s) 65, 76, 242
BstMBI GATC 8 cut(s) 142, 346, 429, 457, 612, 986, 1008, 1044
BstMWI GCNNNNNNNGC 3 cut(s) 472, 1104, 1213
BstNI CCWGG 2 cut(s) 76, 666
BstSCI CCNGG 3 cut(s) 74, 664, 1254
BstSFI CTRYAG 6 cut(s) 106, 565, 855, 882, 933, 1161
BstSLI GKGCMC 1 cut(s) 25
BstV1I GCAGC 4 cut(s) 551, 755, 911, 947
BstX2I RGATCY 4 cut(s) 142, 612, 986, 1008
BstYI RGATCY 4 cut(s) 142, 612, 986, 1008
BstZ17I GTATAC 1 cut(s) 1093
Bsu15I ATCGAT 1 cut(s) 1047
BsuRI GGCC 1 cut(s) 194
BsuTUI ATCGAT 1 cut(s) 1047
BtsCI GGATG 2 cut(s) 591, 783
BveI ACCTGC 1 cut(s) 1168
Cac8I GCNNGC 4 cut(s) 116, 196, 322, 1109
CciI TCATGA 2 cut(s) 201, 454
CfoI GCGC 2 cut(s) 466, 966
Cfr10I RCCGGY 2 cut(s) 118, 950
Cfr13I GGNCC 4 cut(s) 269, 941, 995, 1252
ClaI ATCGAT 1 cut(s) 1047
Csp6I GTAC 4 cut(s) 384, 447, 548, 1039
CspAI ACCGGT 1 cut(s) 950
CviAII CATG 5 cut(s) 202, 455, 461, 1099, 1136
CviQI GTAC 4 cut(s) 384, 447, 548, 1039
DdeI CTNAG 3 cut(s) 12, 719, 957
DpnI GATC 8 cut(s) 144, 348, 431, 459, 614, 988, 1010, 1046
DpnII GATC 8 cut(s) 142, 346, 429, 457, 612, 986, 1008, 1044
Eam1104I CTCTTC 2 cut(s) 391, 1305
EarI CTCTTC 2 cut(s) 391, 1305
Ecl136II GAGCTC 1 cut(s) 475
Eco130I CCWWGG 2 cut(s) 265, 354
Eco24I GRGCYC 3 cut(s) 477, 922, 1109
Eco31I GGTCTC 2 cut(s) 65, 242
Eco32I GATATC 1 cut(s) 289
Eco47I GGWCC 4 cut(s) 269, 941, 995, 1252
Eco53kI GAGCTC 1 cut(s) 475
Eco57I CTGAAG 1 cut(s) 504
Eco88I CYCGRG 1 cut(s) 876
EcoICRI GAGCTC 1 cut(s) 475
EcoRII CCWGG 2 cut(s) 74, 664
EcoRV GATATC 1 cut(s) 289
EcoT14I CCWWGG 2 cut(s) 265, 354
EcoT38I GRGCYC 3 cut(s) 477, 922, 1109
ErhI CCWWGG 2 cut(s) 265, 354
FaeI CATG 5 cut(s) 205, 458, 464, 1102, 1139
FalI AAGNNNNNCTT 2 cut(s) 297, 329
FaqI GGGAC 1 cut(s) 139
FatI CATG 5 cut(s) 201, 454, 460, 1098, 1135
FauI CCCGC 1 cut(s) 1116
FauNDI CATATG 2 cut(s) 436, 443
FbaI TGATCA 1 cut(s) 457
FblI GTMKAC 1 cut(s) 1092
Fnu4HI GCNGC 5 cut(s) 57, 565, 744, 900, 936
FokI GGATG 2 cut(s) 598, 770
FriOI GRGCYC 3 cut(s) 477, 922, 1109
Fsp4HI GCNGC 5 cut(s) 57, 565, 744, 900, 936
FspBI CTAG 2 cut(s) 245, 903
GlaI GCGC 2 cut(s) 465, 965
GluI GCNGC 5 cut(s) 57, 565, 744, 900, 936
GsaI CCCAGC 2 cut(s) 899, 918
GsuI CTGGAG 2 cut(s) 850, 1133
HaeIII GGCC 1 cut(s) 194
HapII CCGG 5 cut(s) 119, 407, 769, 951, 1255
HhaI GCGC 2 cut(s) 466, 966
Hin1II CATG 5 cut(s) 205, 458, 464, 1102, 1139
Hin6I GCGC 2 cut(s) 464, 964
HinP1I GCGC 2 cut(s) 464, 964
HindIII AAGCTT 1 cut(s) 755
HinfI GANTC 7 cut(s) 7, 35, 47, 84, 341, 413, 420
HpaII CCGG 5 cut(s) 119, 407, 769, 951, 1255
HphI GGTGA 4 cut(s) 1114, 1142, 1270, 1285
Hpy166II GTNNAC 4 cut(s) 23, 103, 1039, 1093
Hpy188I TCNGA 5 cut(s) 234, 346, 523, 960, 986
Hpy188III TCNNGA 8 cut(s) 11, 88, 202, 272, 417, 455, 689, 1015
Hpy8I GTNNAC 4 cut(s) 23, 103, 1039, 1093
HpyAV CCTTC 3 cut(s) 299, 528, 901
HpyCH4III ACNGT 3 cut(s) 130, 547, 1187
HpyCH4IV ACGT 1 cut(s) 207
HpyF10VI GCNNNNNNNGC 3 cut(s) 472, 1104, 1213
HpyF3I CTNAG 3 cut(s) 12, 719, 957
HpySE526I ACGT 1 cut(s) 207
Hsp92II CATG 5 cut(s) 205, 458, 464, 1102, 1139
HspAI GCGC 2 cut(s) 464, 964
Ksp22I TGATCA 1 cut(s) 457
Kzo9I GATC 8 cut(s) 142, 346, 429, 457, 612, 986, 1008, 1044
LmnI GCTCC 5 cut(s) 409, 472, 480, 1024, 1114
Lsp1109I GCAGC 4 cut(s) 551, 755, 911, 947
LweI GCATC 2 cut(s) 717, 1156
MaeI CTAG 2 cut(s) 245, 903
MaeII ACGT 1 cut(s) 207
MaeIII GTNAC 1 cut(s) 1129
MalI GATC 8 cut(s) 144, 348, 431, 459, 614, 988, 1010, 1046
MboI GATC 8 cut(s) 142, 346, 429, 457, 612, 986, 1008, 1044
MfeI CAATTG 2 cut(s) 147, 390
MflI RGATCY 4 cut(s) 142, 612, 986, 1008
MhlI GDGCHC 6 cut(s) 25, 477, 922, 958, 1029, 1109
MluCI AATT 4 cut(s) 147, 390, 1154, 1234
MlyI GAGTC 2 cut(s) 16, 78
MmeI TCCRAC 3 cut(s) 407, 566, 1054
MroXI GAANNNNTTC 1 cut(s) 309
MseI TTAA 4 cut(s) 293, 327, 759, 1226
MslI CAYNNNNRTG 4 cut(s) 459, 640, 1032, 1034
MspA1I CMGCKG 3 cut(s) 914, 938, 1111
MspI CCGG 5 cut(s) 119, 407, 769, 951, 1255
MspR9I CCNGG 3 cut(s) 76, 666, 1256
MunI CAATTG 2 cut(s) 147, 390
MvaI CCWGG 2 cut(s) 76, 666
MwoI GCNNNNNNNGC 3 cut(s) 472, 1104, 1213
NciI CCSGG 1 cut(s) 1256
NdeI CATATG 2 cut(s) 436, 443
NdeII GATC 8 cut(s) 142, 346, 429, 457, 612, 986, 1008, 1044
NlaIII CATG 5 cut(s) 205, 458, 464, 1102, 1139
NlaIV GGNNCC 2 cut(s) 144, 1010
NmeAIII GCCGAG 1 cut(s) 178
PaeR7I CTCGAG 1 cut(s) 876
PagI TCATGA 2 cut(s) 201, 454
PcsI WCGNNNNNNNCGW 1 cut(s) 1059
PdmI GAANNNNTTC 1 cut(s) 309
PfeI GAWTC 5 cut(s) 35, 47, 341, 413, 420
PflMI CCANNNNNTGG 1 cut(s) 1207
PinAI ACCGGT 1 cut(s) 950
PkrI GCNGC 5 cut(s) 58, 566, 745, 901, 937
PleI GAGTC 2 cut(s) 15, 78
PpsI GAGTC 2 cut(s) 15, 78
Psp124BI GAGCTC 1 cut(s) 477
Psp1406I AACGTT 1 cut(s) 207
Psp6I CCWGG 2 cut(s) 74, 664
PspFI CCCAGC 2 cut(s) 895, 914
PspGI CCWGG 2 cut(s) 74, 664
PspN4I GGNNCC 2 cut(s) 144, 1010
PspPI GGNCC 4 cut(s) 269, 941, 995, 1252
PspXI VCTCGAGB 1 cut(s) 876
PstI CTGCAG 3 cut(s) 569, 937, 1165
PsuI RGATCY 4 cut(s) 142, 612, 986, 1008
PvuII CAGCTG 2 cut(s) 914, 938
RsaI GTAC 4 cut(s) 385, 448, 549, 1040
RsaNI GTAC 4 cut(s) 384, 447, 548, 1039
RseI CAYNNNNRTG 4 cut(s) 459, 640, 1032, 1034
SacI GAGCTC 1 cut(s) 477
SaqAI TTAA 4 cut(s) 293, 327, 759, 1226
SatI GCNGC 5 cut(s) 57, 565, 744, 900, 936
Sau3AI GATC 8 cut(s) 142, 346, 429, 457, 612, 986, 1008, 1044
Sau96I GGNCC 4 cut(s) 269, 941, 995, 1252
ScaI AGTACT 1 cut(s) 549
SchI GAGTC 2 cut(s) 16, 78
ScrFI CCNGG 3 cut(s) 76, 666, 1256
SduI GDGCHC 6 cut(s) 25, 477, 922, 958, 1029, 1109
SfaNI GCATC 2 cut(s) 717, 1156
SfcI CTRYAG 6 cut(s) 106, 565, 855, 882, 933, 1161
Sfr274I CTCGAG 1 cut(s) 876
SinI GGWCC 4 cut(s) 269, 941, 995, 1252
SlaI CTCGAG 1 cut(s) 876
SmiMI CAYNNNNRTG 4 cut(s) 459, 640, 1032, 1034
SmlI CTYRAG 4 cut(s) 86, 689, 876, 1118
SmoI CTYRAG 4 cut(s) 86, 689, 876, 1118
Sse9I AATT 4 cut(s) 147, 390, 1154, 1234
SsiI CCGC 3 cut(s) 56, 387, 1109
SspI AATATT 1 cut(s) 1224
SspMI CTAG 2 cut(s) 245, 903
SstI GAGCTC 1 cut(s) 477
StyD4I CCNGG 3 cut(s) 74, 664, 1254
StyI CCWWGG 2 cut(s) 265, 354
TaaI ACNGT 3 cut(s) 130, 547, 1187
TaiI ACGT 1 cut(s) 210
TaqI TCGA 2 cut(s) 877, 1047
TasI AATT 4 cut(s) 147, 390, 1154, 1234
TatI WGTACW 3 cut(s) 446, 547, 1038
TauI GCSGC 1 cut(s) 59
TfiI GAWTC 5 cut(s) 35, 47, 341, 413, 420
Tru1I TTAA 4 cut(s) 293, 327, 759, 1226
Tru9I TTAA 4 cut(s) 293, 327, 759, 1226
TseI GCWGC 4 cut(s) 564, 743, 899, 935
TspDTI ATGAA 5 cut(s) 218, 580, 836, 897, 1071
TspGWI ACGGA 1 cut(s) 155
Van91I CCANNNNNTGG 1 cut(s) 1207
VneI GTGCAC 1 cut(s) 21
VpaK11BI GGWCC 4 cut(s) 269, 941, 995, 1252
XhoI CTCGAG 1 cut(s) 876
XmiI GTMKAC 1 cut(s) 1092
XmnI GAANNNNTTC 1 cut(s) 309
XspI CTAG 2 cut(s) 245, 903
ZrmI AGTACT 1 cut(s) 549
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.