Rw1G025690

Alkaline and neutral invertase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0008212
Physical Location & Seq
Forward (+)
52445201 .. 52448872
3672 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G025690.1

Sequence Viewer

Length: 2010 bp
ATGTCGAAACCGCTCGGAGAATACCTCAAGGAAGAAAAGGAAGCAGAGGAGGCGGCCGCTCGGCCTCCGCCTTCTGTTCATGAGACGTTGCCGGAGATAGACTCGCCGGGATCGGATCCATCGATGAAGCTTTCGGATTTCACCGCTGATGACGACTTGGTGTCTTTCGGGCTTCAGCGGCTCAAGGAGATTGCTGAAGCAGCCGAGAAGAAGAAGAAGGCGGAGAAGAATAAGAAGAAGAAGGAGAAGAAGAAGATGAAGAAGCTGCAGGCGGAGAAAGATGTCGGCTCTTCCAAGACTGAAGGTGCGAGTGCGACTCCGAGTGATGAGGATGACTCGACGAAATCGGAAGAAGGTTCTGGAACCTGTGAGAAGAGCGAGAGTGCCGAATTGGCCAAGAAAGAACCATTGTCGCCGGAGAAGTGTACGGGAACGTCCGCGACGCCGCCGTCTACAGGGAAGGCAAAGAAGTCGGTGAAACTGAAACTGCAGAGGCCCGACGAAGCTCCGAAGCAGACGACGTCGCTGCTGATGTCGTTTCAAAGTATGAGGAGTCCTGAGTTGAGTGCACTCATCGTGGAATCTTCGAAGGAATCTTCGGCGGCTATGGTGGAGGAGGCCTGGGAGAGACTCAAGAAGTCGTACGTTTACTACAGAGGCAAGCCGGTGGGGACTCTTGCTGCTATGGATCCAACGGCCGAGGCTCTAAACTACAATCAGGTATTTGTGAGAGATTTTGTTCCTACTGGTTTGGCCTGCCTCATGAAGAATCCAGCAGAGCCAGAGATAGTAAAGAACTTTCTCCTAAAGACCCTCCACCTCCAAGGTCGAGAGAAAATGGTTGATAACTTTACACTTGGAGAAGGTGTTCTTCCTGCGAGCTTTAAGGTTTCAAATGATTCAGATTGTGCCAAGGAAACTATAGTTGCAGATTTTGGTGGTAGCGCAATTGGAAGAGTTGCTCCGGTTGATTCTGGATTCTGGTGGATCATATTGCTGTGGTCATATAACAGATGCACTCGTGATCATGCGCTGGTGGAGCTCCCTGCTGTGCAAAATGGAATGAAACTGATACTCAACCTCTGCCTTTCAGATGGCTTTGACACATTTCCAACACTTCTTTGTGCAGATGGCTGCAGTATGATTGACAGGAGGATGGGCATATATGGTTATCCAATTGAGATCCAAGCACTTTTCTACTTTGCATTGAGATGTGCAATGAAGCTGTTGAAGCCAGAGCGTGGTGGTAAGGAATTTCTTGAGAGGATAGATAAGCGCATTATAGCTCTCAGCTTTCACATCCAGAAGTACTACTGGCTTGACTTTACTCATCTCAATAACATATACCGCTACAAAACAGAGGAGTATTCCCACACAGCTGTCAACAAGTTCAATGTGATGCCAGAGTCCATCCCTGATTGGGTTTTTGATTTCATGCCATTGCGAGGAGGGTATTTGATTGGCAATGTTAGCCCAGCTCGCATGGATTTCCGGTGGTTCTTAGTTGGGAACTGCATTGCAATTCTTAGCTGTTTAGCCACACCTGATCAAGCTACAGCCATAATGGATCTGATTGAGGAGCGTTGGGAGGATTTGATCGGTGAGATGCCTCTGAAAATTGCATATCCAGCATTGGAGGGACATGAGTGGAGGACAATCACAGGATATGACCCCAAAAATACACGATGGAGCTACCACAATGGCGGTTCCTGGCCAACACTGCTGTGGCTGCTGGCTGCGGCTTGCATCAAGACTGGGAGGCCTCAGATTGCAAAGAGGGCTATAGAGCTGGCAGAGCAGCGGCTCTCCAAAGACGGGTGGCCTGAGTACTATGATGGTAAGACAGGGAGATACATTGGGAAGCAAGCGAGGAGGTACCAGACATGGAGCATCTCTGGCTATTTGATTGCTAAGAAGATGATAGAGAATCCATCCAATCTTCTATTGGTTTCTCTTGAGGAGGACAAGAAAATTGCCAAGCCAAGGCTTGCCCGTTCTGCTACTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_100

Protein Analysis

669

Amino Acids

75.08

Weight (kDa)

8.05

Isoelectric Point (pI)

48.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_100 PF12899 207 - 644 3.9e-212 Alkaline and neutral invertase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 448
AatII GACGTC 1 cut(s) 524
Acc65I GGTACC 1 cut(s) 1875
AccB1I GGYRCC 1 cut(s) 1875
AccB7I CCANNNNNTGG 1 cut(s) 1241
AccBSI CCGCTC 2 cut(s) 13, 59
AccI GTMKAC 1 cut(s) 452
AccII CGCG 1 cut(s) 440
AclWI GGATC 8 cut(s) 110, 118, 123, 683, 696, 995, 1177, 1575
AcoI YGGCCR 4 cut(s) 54, 393, 696, 1712
AcsI RAATTY 1 cut(s) 1253
AcuI CTGAAG 3 cut(s) 158, 216, 321
AcyI GRCGYC 2 cut(s) 443, 521
AfaI GTAC 5 cut(s) 427, 644, 1310, 1829, 1877
AfiI CCNNNNNNNGG 6 cut(s) 455, 1241, 1420, 1445, 1815, 1983
AgsI TTSAA 4 cut(s) 542, 894, 1231, 1393
AhdI GACNNNNNGTC 1 cut(s) 160
AjnI CCWGG 2 cut(s) 620, 1709
AleI CACNNNNGTG 1 cut(s) 1723
AloI GAACNNNNNNTCC 2 cut(s) 852, 884
Alw21I GWGCWC 2 cut(s) 571, 1044
Alw26I GTCTC 2 cut(s) 77, 622
Alw44I GTGCAC 1 cut(s) 567
AlwI GGATC 8 cut(s) 110, 118, 123, 683, 696, 995, 1177, 1575
ApaLI GTGCAC 1 cut(s) 567
ApeKI GCWGC 8 cut(s) 200, 265, 526, 680, 1134, 1729, 1736, 1798
ApoI RAATTY 1 cut(s) 1253
Asp700I GAANNNNTTC 1 cut(s) 867
Asp718I GGTACC 1 cut(s) 1875
AspLEI GCGC 3 cut(s) 947, 1033, 1278
AspS9I GGNCC 1 cut(s) 495
AsuC2I CCSGG 1 cut(s) 108
AsuHPI GGTGA 3 cut(s) 133, 487, 1613
AsuII TTCGAA 1 cut(s) 587
BaeGI GKGCMC 1 cut(s) 571
BalI TGGCCA 2 cut(s) 395, 1714
BamHI GGATCC 2 cut(s) 115, 688
BanI GGYRCC 1 cut(s) 1875
BanII GRGCYC 1 cut(s) 1044
BauI CACGAG 1 cut(s) 1020
Bbv12I GWGCWC 2 cut(s) 571, 1044
BbvI GCAGC 8 cut(s) 212, 252, 513, 667, 1121, 1716, 1723, 1810
BccI CCATC 8 cut(s) 127, 1088, 1124, 1150, 1418, 1680, 1829, 1939
BceAI ACGGC 2 cut(s) 433, 711
BcgI CGANNNNNNTGC 4 cut(s) 453, 487, 508, 542
BciT130I CCWGG 2 cut(s) 622, 1711
BclI TGATCA 2 cut(s) 1024, 1546
BcnI CCSGG 1 cut(s) 108
BcoDI GTCTC 2 cut(s) 77, 622
BfmI CTRYAG 8 cut(s) 266, 453, 488, 652, 921, 1135, 1554, 1782
BglI GCCNNNNNGGC 1 cut(s) 392
BmcAI AGTACT 2 cut(s) 1310, 1829
Bme1390I CCNGG 3 cut(s) 108, 622, 1711
BmeRI GACNNNNNGTC 1 cut(s) 160
BmgT120I GGNCC 1 cut(s) 495
BmiI GGNNCC 5 cut(s) 117, 364, 690, 1708, 1877
BmrFI CCNGG 3 cut(s) 108, 622, 1711
BmrI ACTGGG 1 cut(s) 1764
BmsI GCATC 5 cut(s) 1004, 1389, 1596, 1755, 1899
BmuI ACTGGG 1 cut(s) 1764
BplI GAGNNNNNCTC 8 cut(s) 9, 41, 86, 118, 301, 333, 320, 352
Bpu14I TTCGAA 1 cut(s) 587
BpuEI CTTGAG 5 cut(s) 11, 167, 617, 1280, 1976
BpuMI CCSGG 1 cut(s) 108
Bsa29I ATCGAT 1 cut(s) 122
BsaHI GRCGYC 2 cut(s) 443, 521
BsaJI CCNNGG 5 cut(s) 621, 699, 823, 912, 1982
BsaWI WCCGGW 2 cut(s) 964, 1491
BsaXI ACNNNNNCTCC 2 cut(s) 852, 882
Bsc4I CCNNNNNNNGG 6 cut(s) 455, 1241, 1420, 1445, 1815, 1983
Bse118I RCCGGY 1 cut(s) 664
Bse1I ACTGG 3 cut(s) 751, 1319, 1759
Bse3DI GCAATG 4 cut(s) 1224, 1439, 1471, 1515
BseBI CCWGG 2 cut(s) 622, 1711
BseCI ATCGAT 1 cut(s) 122
BseDI CCNNGG 5 cut(s) 621, 699, 823, 912, 1982
BseGI GGATG 5 cut(s) 337, 1161, 1299, 1410, 1931
BseLI CCNNNNNNNGG 6 cut(s) 455, 1241, 1420, 1445, 1815, 1983
BseMI GCAATG 4 cut(s) 1224, 1439, 1471, 1515
BseMII CTCAG 4 cut(s) 549, 1303, 1778, 1815
BseNI ACTGG 3 cut(s) 751, 1319, 1759
BseRI GAGGAG 8 cut(s) 62, 565, 629, 1376, 1461, 1592, 1885, 1973
BseSI GKGCMC 1 cut(s) 571
BseX3I CGGCCG 2 cut(s) 54, 696
BseXI GCAGC 8 cut(s) 212, 252, 513, 667, 1121, 1716, 1723, 1810
BseYI CCCAGC 1 cut(s) 1474
BsgI GTGCAG 1 cut(s) 1146
Bsh1236I CGCG 1 cut(s) 440
Bsh1285I CGRYCG 2 cut(s) 57, 699
BshNI GGYRCC 1 cut(s) 1875
BshVI ATCGAT 1 cut(s) 122
BsiEI CGRYCG 2 cut(s) 57, 699
BsiHKAI GWGCWC 2 cut(s) 571, 1044
BsiSI CCGG 6 cut(s) 92, 107, 416, 665, 965, 1492
BsiWI CGTACG 1 cut(s) 642
BslFI GGGAC 2 cut(s) 685, 1653
BslI CCNNNNNNNGG 6 cut(s) 455, 1241, 1420, 1445, 1815, 1983
BsmAI GTCTC 2 cut(s) 77, 622
BsmBI CGTCTC 1 cut(s) 77
BsmFI GGGAC 2 cut(s) 685, 1653
Bsp119I TTCGAA 1 cut(s) 587
Bsp1286I GDGCHC 2 cut(s) 571, 1044
Bsp143I GATC 9 cut(s) 110, 115, 688, 987, 1024, 1182, 1546, 1567, 1596
BspCNI CTCAG 4 cut(s) 550, 1302, 1777, 1816
BspDI ATCGAT 1 cut(s) 122
BspFNI CGCG 1 cut(s) 440
BspHI TCATGA 2 cut(s) 79, 762
BspLI GGNNCC 5 cut(s) 117, 364, 690, 1708, 1877
BspMAI CTGCAG 3 cut(s) 270, 492, 1139
BspPI GGATC 8 cut(s) 110, 118, 123, 683, 696, 995, 1177, 1575
BspQI GCTCTTC 2 cut(s) 295, 368
BspT104I TTCGAA 1 cut(s) 587
BspT107I GGYRCC 1 cut(s) 1875
BsrBI CCGCTC 2 cut(s) 13, 59
BsrDI GCAATG 4 cut(s) 1224, 1439, 1471, 1515
BsrFI RCCGGY 1 cut(s) 664
BsrI ACTGG 3 cut(s) 751, 1319, 1759
BssAI RCCGGY 1 cut(s) 664
BssECI CCNNGG 5 cut(s) 621, 699, 823, 912, 1982
BssMI GATC 9 cut(s) 110, 115, 688, 987, 1024, 1182, 1546, 1567, 1596
BssNI GRCGYC 2 cut(s) 443, 521
BssSI CACGAG 1 cut(s) 1020
BssT1I CCWWGG 3 cut(s) 823, 912, 1982
Bst2BI CACGAG 1 cut(s) 1020
Bst2UI CCWGG 2 cut(s) 622, 1711
Bst4CI ACNGT 1 cut(s) 2005
Bst6I CTCTTC 3 cut(s) 295, 368, 949
BstACI GRCGYC 2 cut(s) 443, 521
BstBI TTCGAA 1 cut(s) 587
BstDEI CTNAG 7 cut(s) 558, 1289, 1501, 1526, 1764, 1824, 1911
BstF5I GGATG 5 cut(s) 337, 1161, 1299, 1410, 1931
BstFNI CGCG 1 cut(s) 440
BstHHI GCGC 3 cut(s) 947, 1033, 1278
BstKTI GATC 9 cut(s) 113, 118, 691, 990, 1027, 1185, 1549, 1570, 1599
BstMAI GTCTC 2 cut(s) 77, 622
BstMBI GATC 9 cut(s) 110, 115, 688, 987, 1024, 1182, 1546, 1567, 1596
BstMCI CGRYCG 2 cut(s) 57, 699
BstNI CCWGG 2 cut(s) 622, 1711
BstSCI CCNGG 3 cut(s) 106, 620, 1709
BstSFI CTRYAG 8 cut(s) 266, 453, 488, 652, 921, 1135, 1554, 1782
BstSLI GKGCMC 1 cut(s) 571
BstUI CGCG 1 cut(s) 440
BstV1I GCAGC 8 cut(s) 212, 252, 513, 667, 1121, 1716, 1723, 1810
BstX2I RGATCY 4 cut(s) 115, 688, 1182, 1567
BstYI RGATCY 4 cut(s) 115, 688, 1182, 1567
BstZI CGGCCG 2 cut(s) 54, 696
Bsu15I ATCGAT 1 cut(s) 122
BsuTUI ATCGAT 1 cut(s) 122
BtsCI GGATG 5 cut(s) 337, 1161, 1299, 1410, 1931
BtsI GCAGTG 1 cut(s) 1718
BtsIMutI CAGTG 1 cut(s) 1718
CciI TCATGA 2 cut(s) 79, 762
CciNI GCGGCCGC 1 cut(s) 54
CfoI GCGC 3 cut(s) 947, 1033, 1278
Cfr10I RCCGGY 1 cut(s) 664
Cfr13I GGNCC 1 cut(s) 495
ClaI ATCGAT 1 cut(s) 122
CseI GACGC 1 cut(s) 451
Csp6I GTAC 5 cut(s) 426, 643, 1309, 1828, 1876
CviAII CATG 7 cut(s) 80, 763, 1028, 1435, 1483, 1643, 1884
CviQI GTAC 5 cut(s) 426, 643, 1309, 1828, 1876
DdeI CTNAG 7 cut(s) 558, 1289, 1501, 1526, 1764, 1824, 1911
DpnI GATC 9 cut(s) 112, 117, 690, 989, 1026, 1184, 1548, 1569, 1598
DpnII GATC 9 cut(s) 110, 115, 688, 987, 1024, 1182, 1546, 1567, 1596
DrdI GACNNNNNNGTC 1 cut(s) 448
DriI GACNNNNNGTC 1 cut(s) 160
DseDI GACNNNNNNGTC 1 cut(s) 448
EaeI YGGCCR 4 cut(s) 54, 393, 696, 1712
EagI CGGCCG 2 cut(s) 54, 696
Eam1104I CTCTTC 3 cut(s) 295, 368, 949
Eam1105I GACNNNNNGTC 1 cut(s) 160
EarI CTCTTC 3 cut(s) 295, 368, 949
EciI GGCGGA 3 cut(s) 57, 236, 287
Ecl136II GAGCTC 1 cut(s) 1042
EclXI CGGCCG 2 cut(s) 54, 696
Eco130I CCWWGG 3 cut(s) 823, 912, 1982
Eco147I AGGCCT 2 cut(s) 620, 1762
Eco24I GRGCYC 1 cut(s) 1044
Eco52I CGGCCG 2 cut(s) 54, 696
Eco53kI GAGCTC 1 cut(s) 1042
Eco57I CTGAAG 3 cut(s) 158, 216, 321
EcoICRI GAGCTC 1 cut(s) 1042
EcoRII CCWGG 2 cut(s) 620, 1709
EcoT14I CCWWGG 3 cut(s) 823, 912, 1982
EcoT38I GRGCYC 1 cut(s) 1044
ErhI CCWWGG 3 cut(s) 823, 912, 1982
Esp3I CGTCTC 1 cut(s) 77
FaeI CATG 7 cut(s) 83, 766, 1031, 1438, 1486, 1646, 1887
FalI AAGNNNNNCTT 2 cut(s) 855, 887
FaqI GGGAC 2 cut(s) 685, 1653
FatI CATG 7 cut(s) 79, 762, 1027, 1434, 1482, 1642, 1883
FbaI TGATCA 2 cut(s) 1024, 1546
FblI GTMKAC 1 cut(s) 452
FokI GGATG 5 cut(s) 344, 1168, 1286, 1397, 1918
FriOI GRGCYC 1 cut(s) 1044
GlaI GCGC 3 cut(s) 946, 1032, 1277
GsaI CCCAGC 1 cut(s) 1478
HapII CCGG 6 cut(s) 92, 107, 416, 665, 965, 1492
HgaI GACGC 1 cut(s) 451
HhaI GCGC 3 cut(s) 947, 1033, 1278
Hin1I GRCGYC 2 cut(s) 443, 521
Hin1II CATG 7 cut(s) 83, 766, 1031, 1438, 1486, 1646, 1887
Hin6I GCGC 3 cut(s) 945, 1031, 1276
HinP1I GCGC 3 cut(s) 945, 1031, 1276
HincII GTYRAC 1 cut(s) 1384
HindII GTYRAC 1 cut(s) 1384
HindIII AAGCTT 1 cut(s) 128
HpaII CCGG 6 cut(s) 92, 107, 416, 665, 965, 1492
HphI GGTGA 3 cut(s) 133, 487, 1613
Hpy166II GTNNAC 5 cut(s) 426, 453, 569, 649, 1384
Hpy8I GTNNAC 5 cut(s) 426, 453, 569, 649, 1384
Hpy99I CGWCG 5 cut(s) 343, 445, 503, 523, 526
HpyAV CCTTC 8 cut(s) 81, 211, 235, 296, 347, 454, 583, 857
HpyCH4III ACNGT 1 cut(s) 2005
HpyCH4IV ACGT 4 cut(s) 86, 434, 521, 645
HpyF3I CTNAG 7 cut(s) 558, 1289, 1501, 1526, 1764, 1824, 1911
HpySE526I ACGT 4 cut(s) 86, 434, 521, 645
Hsp92I GRCGYC 2 cut(s) 443, 521
Hsp92II CATG 7 cut(s) 83, 766, 1031, 1438, 1486, 1646, 1887
HspAI GCGC 3 cut(s) 945, 1031, 1276
KpnI GGTACC 1 cut(s) 1879
Ksp22I TGATCA 2 cut(s) 1024, 1546
Kzo9I GATC 9 cut(s) 110, 115, 688, 987, 1024, 1182, 1546, 1567, 1596
LguI GCTCTTC 2 cut(s) 295, 368
LmnI GCTCC 7 cut(s) 511, 967, 1039, 1047, 1579, 1689, 1887
Lsp1109I GCAGC 8 cut(s) 212, 252, 513, 667, 1121, 1716, 1723, 1810
LweI GCATC 5 cut(s) 1004, 1389, 1596, 1755, 1899
MaeII ACGT 4 cut(s) 86, 434, 521, 645
MalI GATC 9 cut(s) 112, 117, 690, 989, 1026, 1184, 1548, 1569, 1598
MbiI CCGCTC 2 cut(s) 13, 59
MboI GATC 9 cut(s) 110, 115, 688, 987, 1024, 1182, 1546, 1567, 1596
MfeI CAATTG 2 cut(s) 948, 1176
MflI RGATCY 4 cut(s) 115, 688, 1182, 1567
MhlI GDGCHC 2 cut(s) 571, 1044
MlsI TGGCCA 2 cut(s) 395, 1714
MluCI AATT 7 cut(s) 389, 948, 1176, 1253, 1521, 1617, 1971
MluNI TGGCCA 2 cut(s) 395, 1714
MlyI GAGTC 7 cut(s) 95, 310, 329, 562, 624, 667, 1415
MmeI TCCRAC 2 cut(s) 716, 1136
Mox20I TGGCCA 2 cut(s) 395, 1714
MroXI GAANNNNTTC 1 cut(s) 867
MscI TGGCCA 2 cut(s) 395, 1714
MseI TTAA 2 cut(s) 885, 2008
MslI CAYNNNNRTG 2 cut(s) 1210, 1723
Msp20I TGGCCA 2 cut(s) 395, 1714
MspA1I CMGCKG 4 cut(s) 146, 178, 1379, 1801
MspI CCGG 6 cut(s) 92, 107, 416, 665, 965, 1492
MspR9I CCNGG 3 cut(s) 108, 622, 1711
MunI CAATTG 2 cut(s) 948, 1176
MvaI CCWGG 2 cut(s) 622, 1711
MvnI CGCG 1 cut(s) 440
NciI CCSGG 1 cut(s) 108
NdeII GATC 9 cut(s) 110, 115, 688, 987, 1024, 1182, 1546, 1567, 1596
NlaIII CATG 7 cut(s) 83, 766, 1031, 1438, 1486, 1646, 1887
NlaIV GGNNCC 5 cut(s) 117, 364, 690, 1708, 1877
NmeAIII GCCGAG 3 cut(s) 40, 229, 724
NotI GCGGCCGC 1 cut(s) 54
NspV TTCGAA 1 cut(s) 587
OliI CACNNNNGTG 1 cut(s) 1723
PagI TCATGA 2 cut(s) 79, 762
PceI AGGCCT 2 cut(s) 620, 1762
PciSI GCTCTTC 2 cut(s) 295, 368
PcsI WCGNNNNNNNCGW 1 cut(s) 119
PdmI GAANNNNTTC 1 cut(s) 867
PfeI GAWTC 7 cut(s) 581, 593, 769, 899, 971, 978, 1927
Pfl23II CGTACG 1 cut(s) 642
PflFI GACNNNGTC 1 cut(s) 520
PflMI CCANNNNNTGG 1 cut(s) 1241
PleI GAGTC 7 cut(s) 95, 310, 329, 561, 624, 667, 1414
PpsI GAGTC 7 cut(s) 95, 310, 329, 561, 624, 667, 1414
Psp124BI GAGCTC 1 cut(s) 1044
Psp6I CCWGG 2 cut(s) 620, 1709
PspFI CCCAGC 1 cut(s) 1474
PspGI CCWGG 2 cut(s) 620, 1709
PspLI CGTACG 1 cut(s) 642
PspN4I GGNNCC 5 cut(s) 117, 364, 690, 1708, 1877
PspPI GGNCC 1 cut(s) 495
PstI CTGCAG 3 cut(s) 270, 492, 1139
PsuI RGATCY 4 cut(s) 115, 688, 1182, 1567
PsyI GACNNNGTC 1 cut(s) 520
PvuII CAGCTG 1 cut(s) 1379
RsaI GTAC 5 cut(s) 427, 644, 1310, 1829, 1877
RsaNI GTAC 5 cut(s) 426, 643, 1309, 1828, 1876
RseI CAYNNNNRTG 2 cut(s) 1210, 1723
SacI GAGCTC 1 cut(s) 1044
SapI GCTCTTC 2 cut(s) 295, 368
SaqAI TTAA 2 cut(s) 885, 2008
Sau3AI GATC 9 cut(s) 110, 115, 688, 987, 1024, 1182, 1546, 1567, 1596
Sau96I GGNCC 1 cut(s) 495
ScaI AGTACT 2 cut(s) 1310, 1829
SchI GAGTC 7 cut(s) 95, 310, 329, 562, 624, 667, 1415
ScrFI CCNGG 3 cut(s) 108, 622, 1711
SduI GDGCHC 2 cut(s) 571, 1044
SfaNI GCATC 5 cut(s) 1004, 1389, 1596, 1755, 1899
SfcI CTRYAG 8 cut(s) 266, 453, 488, 652, 921, 1135, 1554, 1782
SfuI TTCGAA 1 cut(s) 587
SmiMI CAYNNNNRTG 2 cut(s) 1210, 1723
SmlI CTYRAG 5 cut(s) 26, 182, 632, 1259, 1955
SmoI CTYRAG 5 cut(s) 26, 182, 632, 1259, 1955
Sse9I AATT 7 cut(s) 389, 948, 1176, 1253, 1521, 1617, 1971
SseBI AGGCCT 2 cut(s) 620, 1762
SstI GAGCTC 1 cut(s) 1044
StuI AGGCCT 2 cut(s) 620, 1762
StyD4I CCNGG 3 cut(s) 106, 620, 1709
StyI CCWWGG 3 cut(s) 823, 912, 1982
TaaI ACNGT 1 cut(s) 2005
TaiI ACGT 4 cut(s) 89, 437, 524, 648
TaqI TCGA 5 cut(s) 5, 122, 338, 587, 829
TasI AATT 7 cut(s) 389, 948, 1176, 1253, 1521, 1617, 1971
TatI WGTACW 2 cut(s) 1308, 1827
TauI GCSGC 7 cut(s) 56, 59, 181, 448, 605, 1742, 1804
TfiI GAWTC 7 cut(s) 581, 593, 769, 899, 971, 978, 1927
Tru1I TTAA 2 cut(s) 885, 2008
Tru9I TTAA 2 cut(s) 885, 2008
TscAI CASTG 1 cut(s) 1725
TseI GCWGC 8 cut(s) 200, 265, 526, 680, 1134, 1729, 1736, 1798
TspDTI ATGAA 7 cut(s) 68, 140, 272, 779, 1079, 1235, 1423
TspRI CASTG 1 cut(s) 1725
Tth111I GACNNNGTC 1 cut(s) 520
Van91I CCANNNNNTGG 1 cut(s) 1241
VneI GTGCAC 1 cut(s) 567
XapI RAATTY 1 cut(s) 1253
XcmI CCANNNNNNNNNTGG 2 cut(s) 1722, 1942
XmiI GTMKAC 1 cut(s) 452
XmnI GAANNNNTTC 1 cut(s) 867
ZraI GACGTC 1 cut(s) 522
ZrmI AGTACT 2 cut(s) 1310, 1829
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.