FvH4_7g19881

General transcription factor IIF subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0013034
Physical Location & Seq
Reverse (-)
16306602 .. 16309678
3077 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g19881.t1

Sequence Viewer

Length: 843 bp
ATGGCGCCAACCCAAGTAGCAATGGAGGACCAAAAACCACTACAACGAACCAGTAGCAGCACCAGTGCCAAAACCTGGGGGCACTTGGAGACCAGCAAAGCGGCGGAGCCGGTGTGGCTCATGAAATGCCCGCCCAATGTCGCCAAATCGTTCAAGGCTCACTCCGCCGAGGACGGCGGAGGCGCGGCGTCGGAGGCTGGTGGCTCCGGCCCCGTCGCCAAGGTCATTCTCTCGCTTGACCCTCTCAACTCCAATAACGAACCCAAGTTCACCATGGAACTGGCGGCAACAGAATCTGCGAATACGCCCAAGTGTTACGTTTTGGATATGTCCAGGGACTTTGTGCCAATGTCTGTGTTTTCGGAGTCATCGGAAGGAAAGTTATCTGTGGAGGGAAAAATCTTCAACAAGTTTGATATGAAACCCCATGATGAGAATCTTGAGAATTATGGGAAACTTTGCCGTGAACGAACAAAAAAGCATATGAAAAGTAGAAAGATTCAGGTGATCGACAATGACAATGGGGCTCATATGAGGCCAATGCCAGGGATGATTGGTTTTGTGGCTCCTGGACCCAGTGATAAGAAGAAAGTGCCAACGAAGGGATCAGAAATGAAGAGAACAAGGAGGGACCGTGGAGAAATGGAAGATATCATGTTTAAGCTCTTTGAGAGGCAACCTAATTGGACATTAAGACAATTGATCCAAGAGACTGATCAACCCGAACAATTTCTGAAAGACATACTTAAAGATCTCTCTGTCTACAACAACAAGGGAGCTAATCAAGGCACATATGAGCTGAAACCAGAATACAGGAGAACAAGCGAGGATGCAGCTCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000375 GO:0000377 GO:0000398 GO:0000428 GO:0000988 GO:0000990 GO:0000991 GO:0003674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0005674 GO:0005856 GO:0006139 GO:0006351 GO:0006352 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006367 GO:0006368 GO:0006370 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0008150 GO:0008152 GO:0008380 GO:0008543 GO:0009058 GO:0009059 GO:0009301 GO:0009452 GO:0009719 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0015630 GO:0016070 GO:0016071 GO:0016073 GO:0016591 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0023052 GO:0030880 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031334 GO:0031974 GO:0031981 GO:0032774 GO:0032784 GO:0032786 GO:0032968 GO:0032991 GO:0032993 GO:0034243 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0036260 GO:0042221 GO:0042795 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044344 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044798 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051716 GO:0055029 GO:0060255 GO:0060260 GO:0060261 GO:0061695 GO:0065007 GO:0070013 GO:0070848 GO:0070887 GO:0071310 GO:0071363 GO:0071495 GO:0071704 GO:0071774 GO:0080090 GO:0090304 GO:0090575 GO:0097550 GO:0097659 GO:0098781 GO:0140110 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1902680 GO:1903506 GO:1903508 GO:1990234 GO:2000112 GO:2000142 GO:2000144 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03022 map03022
Pfam Domains
Protein Families
TFIIF_beta

Protein Analysis

281

Amino Acids

31.3

Weight (kDa)

8.58

Isoelectric Point (pI)

37.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TFIIF_beta PF02270 209 - 272 4.3e-19 TFIIF, beta subunit HTH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013034)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52270
fragaria_vesca FvH4_7g19881
malus_domestica MD01G1111000.v1.1 MD07G1177200.v1.1
prunus_persica Prupe.2G215900_v2.0.a1
pyrus_communis pycom07g17120
rosa_chinensis RchiOBHm_Chr1g0363871
rosa_laevigata RLG00000027539
rosa_multiflora Rmu_sc0002483.1_g000003
rosa_roxburghii Rroxscaffold_4G00292900
rosa_rugosa Rorug01G0310400 Rorug01G0310500 Rorug01G0310600
rosa_samantha Rh1AG318500 Rh1BG281700 Rh1CG298100 Rh1DG313000
rosa_wichuraiana Rw1G028270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 4
AccB7I CCANNNNNTGG 1 cut(s) 75
AccI GTMKAC 1 cut(s) 762
AccII CGCG 1 cut(s) 185
AciI CCGC 7 cut(s) 101, 104, 131, 165, 177, 185, 284
AclWI GGATC 2 cut(s) 613, 697
AcyI GRCGYC 2 cut(s) 5, 188
AfiI CCNNNNNNNGG 3 cut(s) 75, 545, 602
AgsI TTSAA 2 cut(s) 154, 406
AjnI CCWGG 4 cut(s) 74, 332, 544, 568
AluBI AGCT 4 cut(s) 664, 779, 799, 836
AluI AGCT 4 cut(s) 664, 779, 799, 836
Alw26I GTCTC 2 cut(s) 83, 704
AlwI GGATC 2 cut(s) 613, 697
AlwNI CAGNNNCTG 1 cut(s) 296
AoxI GGCC 2 cut(s) 208, 536
ApeKI GCWGC 2 cut(s) 57, 833
Asp700I GAANNNNTTC 1 cut(s) 729
AspLEI GCGC 2 cut(s) 7, 185
AspS9I GGNCC 4 cut(s) 28, 209, 572, 631
AsuHPI GGTGA 2 cut(s) 262, 517
AvaII GGWCC 3 cut(s) 28, 572, 631
BaeGI GKGCMC 1 cut(s) 84
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 529
BbvI GCAGC 1 cut(s) 69
BceAI ACGGC 2 cut(s) 190, 447
BciT130I CCWGG 4 cut(s) 76, 334, 546, 570
BclI TGATCA 1 cut(s) 715
BcoDI GTCTC 2 cut(s) 83, 704
BfoI RGCGCY 1 cut(s) 8
BglI GCCNNNNNGGC 1 cut(s) 115
BglII AGATCT 1 cut(s) 751
BisI GCNGC 5 cut(s) 58, 102, 186, 285, 834
BlsI GCNGC 5 cut(s) 59, 103, 187, 286, 835
Bme1390I CCNGG 4 cut(s) 76, 334, 546, 570
Bme18I GGWCC 3 cut(s) 28, 572, 631
BmgT120I GGNCC 4 cut(s) 28, 209, 572, 631
BmiI GGNNCC 7 cut(s) 6, 108, 205, 211, 567, 574, 632
BmrFI CCNGG 4 cut(s) 76, 334, 546, 570
BmrI ACTGGG 1 cut(s) 570
BmsI GCATC 1 cut(s) 820
BmuI ACTGGG 1 cut(s) 570
BpuEI CTTGAG 1 cut(s) 461
BsaBI GATNNNNATC 1 cut(s) 435
BsaHI GRCGYC 2 cut(s) 5, 188
BsaI GGTCTC 1 cut(s) 83
BsaJI CCNNGG 7 cut(s) 75, 168, 219, 273, 333, 545, 634
BsaXI ACNNNNNCTCC 4 cut(s) 98, 128, 619, 649
Bsc4I CCNNNNNNNGG 3 cut(s) 75, 545, 602
Bse118I RCCGGY 1 cut(s) 109
Bse1I ACTGG 4 cut(s) 51, 63, 285, 576
Bse3DI GCAATG 1 cut(s) 27
Bse8I GATNNNNATC 1 cut(s) 435
BseBI CCWGG 4 cut(s) 76, 334, 546, 570
BseDI CCNNGG 7 cut(s) 75, 168, 219, 273, 333, 545, 634
BseGI GGATG 2 cut(s) 555, 835
BseJI GATNNNNATC 1 cut(s) 435
BseLI CCNNNNNNNGG 3 cut(s) 75, 545, 602
BseMI GCAATG 1 cut(s) 27
BseNI ACTGG 4 cut(s) 51, 63, 285, 576
BseSI GKGCMC 1 cut(s) 84
BseXI GCAGC 1 cut(s) 69
Bsh1236I CGCG 1 cut(s) 185
BshFI GGCC 2 cut(s) 210, 538
BshNI GGYRCC 1 cut(s) 4
BsiSI CCGG 2 cut(s) 110, 207
BslFI GGGAC 2 cut(s) 350, 644
BslI CCNNNNNNNGG 3 cut(s) 75, 545, 602
BsmAI GTCTC 2 cut(s) 83, 704
BsmFI GGGAC 2 cut(s) 350, 644
BsnI GGCC 2 cut(s) 210, 538
Bso31I GGTCTC 1 cut(s) 83
Bsp1286I GDGCHC 2 cut(s) 84, 529
Bsp143I GATC 5 cut(s) 507, 605, 702, 715, 751
Bsp19I CCATGG 1 cut(s) 273
BspACI CCGC 7 cut(s) 101, 104, 131, 165, 177, 185, 284
BspANI GGCC 2 cut(s) 210, 538
BspFNI CGCG 1 cut(s) 185
BspHI TCATGA 1 cut(s) 120
BspLI GGNNCC 7 cut(s) 6, 108, 205, 211, 567, 574, 632
BspPI GGATC 2 cut(s) 613, 697
BspT107I GGYRCC 1 cut(s) 4
BspTNI GGTCTC 1 cut(s) 83
BsrDI GCAATG 1 cut(s) 27
BsrFI RCCGGY 1 cut(s) 109
BsrI ACTGG 4 cut(s) 51, 63, 285, 576
BssAI RCCGGY 1 cut(s) 109
BssECI CCNNGG 7 cut(s) 75, 168, 219, 273, 333, 545, 634
BssMI GATC 5 cut(s) 507, 605, 702, 715, 751
BssNI GRCGYC 2 cut(s) 5, 188
BssT1I CCWWGG 2 cut(s) 219, 273
Bst2UI CCWGG 4 cut(s) 76, 334, 546, 570
Bst4CI ACNGT 1 cut(s) 635
Bst6I CTCTTC 1 cut(s) 611
BstACI GRCGYC 2 cut(s) 5, 188
BstC8I GCNNGC 1 cut(s) 131
BstDSI CCRYGG 2 cut(s) 273, 634
BstF5I GGATG 2 cut(s) 555, 835
BstFNI CGCG 1 cut(s) 185
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 2 cut(s) 7, 185
BstKTI GATC 5 cut(s) 510, 608, 705, 718, 754
BstMAI GTCTC 2 cut(s) 83, 704
BstMBI GATC 5 cut(s) 507, 605, 702, 715, 751
BstMWI GCNNNNNNNGC 3 cut(s) 115, 164, 194
BstNI CCWGG 4 cut(s) 76, 334, 546, 570
BstSCI CCNGG 4 cut(s) 74, 332, 544, 568
BstSLI GKGCMC 1 cut(s) 84
BstUI CGCG 1 cut(s) 185
BstV1I GCAGC 1 cut(s) 69
BstX2I RGATCY 1 cut(s) 751
BstXI CCANNNNNNTGG 1 cut(s) 280
BstYI RGATCY 1 cut(s) 751
BsuRI GGCC 2 cut(s) 210, 538
BtgI CCRYGG 2 cut(s) 273, 634
BtsCI GGATG 2 cut(s) 555, 835
BtsIMutI CAGTG 2 cut(s) 70, 583
Cac8I GCNNGC 1 cut(s) 131
CaiI CAGNNNCTG 1 cut(s) 296
CciI TCATGA 1 cut(s) 120
CfoI GCGC 2 cut(s) 7, 185
Cfr10I RCCGGY 1 cut(s) 109
Cfr13I GGNCC 4 cut(s) 28, 209, 572, 631
CseI GACGC 1 cut(s) 177
CviAII CATG 4 cut(s) 121, 274, 428, 655
DinI GGCGCC 1 cut(s) 6
DpnI GATC 5 cut(s) 509, 607, 704, 717, 753
DpnII GATC 5 cut(s) 507, 605, 702, 715, 751
Eam1104I CTCTTC 1 cut(s) 611
EarI CTCTTC 1 cut(s) 611
EciI GGCGGA 3 cut(s) 119, 154, 192
Eco130I CCWWGG 2 cut(s) 219, 273
Eco24I GRGCYC 1 cut(s) 529
Eco31I GGTCTC 1 cut(s) 83
Eco32I GATATC 1 cut(s) 652
Eco47I GGWCC 3 cut(s) 28, 572, 631
EcoRII CCWGG 4 cut(s) 74, 332, 544, 568
EcoRV GATATC 1 cut(s) 652
EcoT14I CCWWGG 2 cut(s) 219, 273
EcoT38I GRGCYC 1 cut(s) 529
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 2 cut(s) 219, 273
FaeI CATG 4 cut(s) 124, 277, 431, 658
FalI AAGNNNNNCTT 2 cut(s) 729, 761
FaqI GGGAC 2 cut(s) 350, 644
FatI CATG 4 cut(s) 120, 273, 427, 654
FauI CCCGC 1 cut(s) 138
FauNDI CATATG 3 cut(s) 483, 531, 793
FbaI TGATCA 1 cut(s) 715
FblI GTMKAC 1 cut(s) 762
Fnu4HI GCNGC 5 cut(s) 58, 102, 186, 285, 834
FokI GGATG 1 cut(s) 562
FriOI GRGCYC 1 cut(s) 529
Fsp4HI GCNGC 5 cut(s) 58, 102, 186, 285, 834
GlaI GCGC 2 cut(s) 6, 184
GluI GCNGC 5 cut(s) 58, 102, 186, 285, 834
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 2 cut(s) 210, 538
HapII CCGG 2 cut(s) 110, 207
HgaI GACGC 1 cut(s) 177
HhaI GCGC 2 cut(s) 7, 185
Hin1I GRCGYC 2 cut(s) 5, 188
Hin1II CATG 4 cut(s) 124, 277, 431, 658
Hin6I GCGC 2 cut(s) 5, 183
HinP1I GCGC 2 cut(s) 5, 183
HinfI GANTC 4 cut(s) 293, 365, 436, 499
HpaII CCGG 2 cut(s) 110, 207
HphI GGTGA 2 cut(s) 262, 517
Hpy166II GTNNAC 3 cut(s) 270, 467, 763
Hpy188I TCNGA 5 cut(s) 193, 364, 373, 610, 735
Hpy188III TCNNGA 2 cut(s) 121, 440
Hpy8I GTNNAC 3 cut(s) 270, 467, 763
Hpy99I CGWCG 2 cut(s) 193, 218
HpyAV CCTTC 2 cut(s) 368, 595
HpyCH4III ACNGT 1 cut(s) 635
HpyCH4IV ACGT 1 cut(s) 318
HpyCH4V TGCA 1 cut(s) 833
HpyF10VI GCNNNNNNNGC 3 cut(s) 115, 164, 194
HpySE526I ACGT 1 cut(s) 318
Hsp92I GRCGYC 2 cut(s) 5, 188
Hsp92II CATG 4 cut(s) 124, 277, 431, 658
HspAI GCGC 2 cut(s) 5, 183
KasI GGCGCC 1 cut(s) 4
Ksp22I TGATCA 1 cut(s) 715
Kzo9I GATC 5 cut(s) 507, 605, 702, 715, 751
LmnI GCTCC 5 cut(s) 106, 209, 571, 776, 841
Lsp1109I GCAGC 1 cut(s) 69
LweI GCATC 1 cut(s) 820
MaeII ACGT 1 cut(s) 318
MaeIII GTNAC 1 cut(s) 314
MalI GATC 5 cut(s) 509, 607, 704, 717, 753
MboI GATC 5 cut(s) 507, 605, 702, 715, 751
MboII GAAGA 4 cut(s) 394, 598, 628, 659
MfeI CAATTG 1 cut(s) 698
MflI RGATCY 1 cut(s) 751
MhlI GDGCHC 2 cut(s) 84, 529
MluCI AATT 4 cut(s) 445, 682, 698, 728
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 1 cut(s) 374
MmeI TCCRAC 1 cut(s) 171
MroXI GAANNNNTTC 1 cut(s) 729
MseI TTAA 3 cut(s) 660, 692, 747
MspI CCGG 2 cut(s) 110, 207
MspR9I CCNGG 4 cut(s) 76, 334, 546, 570
MunI CAATTG 1 cut(s) 698
MvaI CCWGG 4 cut(s) 76, 334, 546, 570
MvnI CGCG 1 cut(s) 185
MwoI GCNNNNNNNGC 3 cut(s) 115, 164, 194
NarI GGCGCC 1 cut(s) 5
NcoI CCATGG 1 cut(s) 273
NdeI CATATG 3 cut(s) 483, 531, 793
NdeII GATC 5 cut(s) 507, 605, 702, 715, 751
NlaIII CATG 4 cut(s) 124, 277, 431, 658
NlaIV GGNNCC 7 cut(s) 6, 108, 205, 211, 567, 574, 632
NmeAIII GCCGAG 1 cut(s) 193
PagI TCATGA 1 cut(s) 120
PdmI GAANNNNTTC 1 cut(s) 729
PfeI GAWTC 3 cut(s) 293, 436, 499
PflMI CCANNNNNTGG 1 cut(s) 75
PfoI TCCNGGA 1 cut(s) 568
PkrI GCNGC 5 cut(s) 59, 103, 187, 286, 835
PleI GAGTC 1 cut(s) 373
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 1 cut(s) 373
Psp6I CCWGG 4 cut(s) 74, 332, 544, 568
PspGI CCWGG 4 cut(s) 74, 332, 544, 568
PspN4I GGNNCC 7 cut(s) 6, 108, 205, 211, 567, 574, 632
PspPI GGNCC 4 cut(s) 28, 209, 572, 631
PstNI CAGNNNCTG 1 cut(s) 296
PsuI RGATCY 1 cut(s) 751
SaqAI TTAA 3 cut(s) 660, 692, 747
SatI GCNGC 5 cut(s) 58, 102, 186, 285, 834
Sau3AI GATC 5 cut(s) 507, 605, 702, 715, 751
Sau96I GGNCC 4 cut(s) 28, 209, 572, 631
SchI GAGTC 1 cut(s) 374
ScrFI CCNGG 4 cut(s) 76, 334, 546, 570
SduI GDGCHC 2 cut(s) 84, 529
SetI ASST 9 cut(s) 77, 225, 321, 507, 666, 682, 781, 801, 838
SfaNI GCATC 1 cut(s) 820
SfoI GGCGCC 1 cut(s) 6
SinI GGWCC 3 cut(s) 28, 572, 631
SmlI CTYRAG 1 cut(s) 440
SmoI CTYRAG 1 cut(s) 440
Sse9I AATT 4 cut(s) 445, 682, 698, 728
SsiI CCGC 7 cut(s) 101, 104, 131, 165, 177, 185, 284
SspDI GGCGCC 1 cut(s) 4
StyD4I CCNGG 4 cut(s) 74, 332, 544, 568
StyI CCWWGG 2 cut(s) 219, 273
TaaI ACNGT 1 cut(s) 635
TaiI ACGT 1 cut(s) 321
TaqI TCGA 1 cut(s) 510
TasI AATT 4 cut(s) 445, 682, 698, 728
TauI GCSGC 3 cut(s) 104, 188, 287
TfiI GAWTC 3 cut(s) 293, 436, 499
Tru1I TTAA 3 cut(s) 660, 692, 747
Tru9I TTAA 3 cut(s) 660, 692, 747
TscAI CASTG 2 cut(s) 70, 583
TseI GCWGC 2 cut(s) 57, 833
TspDTI ATGAA 4 cut(s) 137, 434, 500, 629
TspRI CASTG 2 cut(s) 70, 583
Van91I CCANNNNNTGG 1 cut(s) 75
VpaK11BI GGWCC 3 cut(s) 28, 572, 631
XcmI CCANNNNNNNNNTGG 1 cut(s) 271
XmiI GTMKAC 1 cut(s) 762
XmnI GAANNNNTTC 1 cut(s) 729
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.