pycom07g17120

General transcription factor IIF subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0013034
Physical Location & Seq
Reverse (-)
19667418 .. 19670062
2645 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g17120.4

Sequence Viewer

Length: 807 bp
ATGGAGGAGCAAAAGCGGCAACAAGGCAATAACAATGTCGTGAATGGGCGTTATTTGGAGACCAACAGAGAAAATATTCCGGTGTTCCTCATGAAATCTCCCCCGCTCGTCTCTCGCTGTCTGAAACCTGATGTCTCCTCCGCCTCCGCTTCTGATCAACAGCAGTCTTCTCCCGCTGTTGCCAAGTTGGTTCTCTCTTTTGACCCTATCGCCGAGGTCAACGAGTTCACCATGGAACTGGCGGCCACGAAATCTGAAAATGTACCCAAGTGTTACGCTTTGGATATGTTTGAAGACGCCCTTCCAATGTCCGTGTTTTCTGAGTCATCGCAAGGGACGTTATCCGTGGAGGGGAAGATATTGCAGAAATTTGATATGAAGCCCCATGGTGAGAACCTTGAGAACTATGGGAAACTTTGCCGCGAAAGAACCAAAAAATATATGAAGCGAAGAAAGATTCAGGTGATGAACAACGACAATGGGGCTCATATGAGGCCCAGGCCAGGGATGATGATTGGTTTCGTGTCGCCTGGACCCAGCGAGAAAAAGAAAATGCCAACGAAGGGGTCAGACATGAAAAGAACAAGGAAGGACCGCGGAGAAATGGAAGATATCATGTTTAATCTCTTTGAACAGAAACCAAATCGGACGTTAAGGCAACTGATCCAAGAGACTAACCAACCAGAACAATTTTTGAAGGACATACTCAAGGATCTTTGTATCTACAACAACAAGGGAGCTAATCAAGGGACGTATGAGCTGAAACCGGAATACAGGAAATCAGTCGAGGAGCCAACTCCGGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000375 GO:0000377 GO:0000398 GO:0000428 GO:0000988 GO:0000990 GO:0000991 GO:0003674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0005674 GO:0005856 GO:0006139 GO:0006351 GO:0006352 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006367 GO:0006368 GO:0006370 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0008150 GO:0008152 GO:0008380 GO:0008543 GO:0009058 GO:0009059 GO:0009301 GO:0009452 GO:0009719 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0015630 GO:0016070 GO:0016071 GO:0016073 GO:0016591 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0023052 GO:0030880 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031334 GO:0031974 GO:0031981 GO:0032774 GO:0032784 GO:0032786 GO:0032968 GO:0032991 GO:0032993 GO:0034243 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0036260 GO:0042221 GO:0042795 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044344 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044798 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051716 GO:0055029 GO:0060255 GO:0060260 GO:0060261 GO:0061695 GO:0065007 GO:0070013 GO:0070848 GO:0070887 GO:0071310 GO:0071363 GO:0071495 GO:0071704 GO:0071774 GO:0080090 GO:0090304 GO:0090575 GO:0097550 GO:0097659 GO:0098781 GO:0140110 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1902680 GO:1903506 GO:1903508 GO:1990234 GO:2000112 GO:2000142 GO:2000144 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03022 map03022
Pfam Domains
Protein Families
TFIIF_beta

Protein Analysis

269

Amino Acids

30.66

Weight (kDa)

8.87

Isoelectric Point (pI)

47.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013034)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52270
fragaria_vesca FvH4_7g19881
malus_domestica MD01G1111000.v1.1 MD07G1177200.v1.1
prunus_persica Prupe.2G215900_v2.0.a1
pyrus_communis pycom07g17120
rosa_chinensis RchiOBHm_Chr1g0363871
rosa_laevigata RLG00000027539
rosa_multiflora Rmu_sc0002483.1_g000003
rosa_roxburghii Rroxscaffold_4G00292900
rosa_rugosa Rorug01G0310400 Rorug01G0310500 Rorug01G0310600
rosa_samantha Rh1AG318500 Rh1BG281700 Rh1CG298100 Rh1DG313000
rosa_wichuraiana Rw1G028270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 106
AccII CGCG 2 cut(s) 423, 597
AccIII TCCGGA 1 cut(s) 799
AciI CCGC 9 cut(s) 16, 104, 141, 147, 174, 242, 421, 595, 597
AclWI GGATC 2 cut(s) 658, 720
AcoI YGGCCR 1 cut(s) 243
AcsI RAATTY 1 cut(s) 368
AcyI GRCGYC 1 cut(s) 297
AfaI GTAC 1 cut(s) 264
AfiI CCNNNNNNNGG 4 cut(s) 351, 503, 504, 563
AgsI TTSAA 3 cut(s) 293, 632, 697
AjnI CCWGG 3 cut(s) 497, 502, 529
AluBI AGCT 2 cut(s) 740, 760
AluI AGCT 2 cut(s) 740, 760
Alw26I GTCTC 4 cut(s) 53, 115, 139, 665
AlwI GGATC 2 cut(s) 658, 720
Aor13HI TCCGGA 1 cut(s) 799
AoxI GGCC 3 cut(s) 243, 494, 500
ApoI RAATTY 1 cut(s) 368
Asp700I GAANNNNTTC 1 cut(s) 75
AspS9I GGNCC 3 cut(s) 495, 533, 592
AsuHPI GGTGA 3 cut(s) 220, 401, 475
AvaII GGWCC 2 cut(s) 533, 592
BanII GRGCYC 1 cut(s) 487
BbsI GAAGAC 2 cut(s) 159, 300
BciT130I CCWGG 3 cut(s) 499, 504, 531
BclI TGATCA 1 cut(s) 154
BcoDI GTCTC 4 cut(s) 53, 115, 139, 665
BisI GCNGC 3 cut(s) 17, 243, 421
BlsI GCNGC 3 cut(s) 18, 244, 422
Bme1390I CCNGG 3 cut(s) 499, 504, 531
Bme18I GGWCC 2 cut(s) 533, 592
BmgT120I GGNCC 3 cut(s) 495, 533, 592
BmiI GGNNCC 2 cut(s) 535, 792
BmrFI CCNGG 3 cut(s) 499, 504, 531
BpiI GAAGAC 2 cut(s) 159, 300
BpuEI CTTGAG 2 cut(s) 419, 692
BsaHI GRCGYC 1 cut(s) 297
BsaI GGTCTC 1 cut(s) 53
BsaJI CCNNGG 7 cut(s) 213, 231, 345, 385, 497, 503, 595
BsaWI WCCGGW 3 cut(s) 79, 766, 799
Bsc4I CCNNNNNNNGG 4 cut(s) 351, 503, 504, 563
Bse1I ACTGG 1 cut(s) 243
BseAI TCCGGA 1 cut(s) 799
BseBI CCWGG 3 cut(s) 499, 504, 531
BseDI CCNNGG 7 cut(s) 213, 231, 345, 385, 497, 503, 595
BseGI GGATG 1 cut(s) 513
BseLI CCNNNNNNNGG 4 cut(s) 351, 503, 504, 563
BseMII CTCAG 1 cut(s) 312
BseNI ACTGG 1 cut(s) 243
BseRI GAGGAG 3 cut(s) 20, 127, 803
BseYI CCCAGC 1 cut(s) 536
Bsh1236I CGCG 2 cut(s) 423, 597
BshFI GGCC 3 cut(s) 245, 496, 502
BsiSI CCGG 3 cut(s) 80, 767, 800
BslFI GGGAC 2 cut(s) 349, 763
BslI CCNNNNNNNGG 4 cut(s) 351, 503, 504, 563
BsmAI GTCTC 4 cut(s) 53, 115, 139, 665
BsmBI CGTCTC 1 cut(s) 115
BsmFI GGGAC 2 cut(s) 349, 763
BsnI GGCC 3 cut(s) 245, 496, 502
Bso31I GGTCTC 1 cut(s) 53
Bsp1286I GDGCHC 1 cut(s) 487
Bsp13I TCCGGA 1 cut(s) 799
Bsp143I GATC 3 cut(s) 154, 663, 712
Bsp19I CCATGG 2 cut(s) 231, 385
BspACI CCGC 9 cut(s) 16, 104, 141, 147, 174, 242, 421, 595, 597
BspANI GGCC 3 cut(s) 245, 496, 502
BspCNI CTCAG 1 cut(s) 313
BspEI TCCGGA 1 cut(s) 799
BspFNI CGCG 2 cut(s) 423, 597
BspHI TCATGA 1 cut(s) 90
BspLI GGNNCC 2 cut(s) 535, 792
BspPI GGATC 2 cut(s) 658, 720
BspTNI GGTCTC 1 cut(s) 53
BsrBI CCGCTC 1 cut(s) 106
BsrI ACTGG 1 cut(s) 243
BssECI CCNNGG 7 cut(s) 213, 231, 345, 385, 497, 503, 595
BssMI GATC 3 cut(s) 154, 663, 712
BssNI GRCGYC 1 cut(s) 297
BssT1I CCWWGG 2 cut(s) 231, 385
Bst2UI CCWGG 3 cut(s) 499, 504, 531
BstACI GRCGYC 1 cut(s) 297
BstDEI CTNAG 1 cut(s) 321
BstDSI CCRYGG 4 cut(s) 231, 345, 385, 595
BstF5I GGATG 1 cut(s) 513
BstFNI CGCG 2 cut(s) 423, 597
BstKTI GATC 3 cut(s) 157, 666, 715
BstMAI GTCTC 4 cut(s) 53, 115, 139, 665
BstMBI GATC 3 cut(s) 154, 663, 712
BstMWI GCNNNNNNNGC 1 cut(s) 16
BstNI CCWGG 3 cut(s) 499, 504, 531
BstSCI CCNGG 3 cut(s) 497, 502, 529
BstUI CGCG 2 cut(s) 423, 597
BstV2I GAAGAC 2 cut(s) 159, 300
BstX2I RGATCY 1 cut(s) 712
BstXI CCANNNNNNTGG 1 cut(s) 238
BstYI RGATCY 1 cut(s) 712
BsuRI GGCC 3 cut(s) 245, 496, 502
BtgI CCRYGG 4 cut(s) 231, 345, 385, 595
BtgZI GCGATG 1 cut(s) 312
BtsCI GGATG 1 cut(s) 513
CciI TCATGA 1 cut(s) 90
Cfr13I GGNCC 3 cut(s) 495, 533, 592
Cfr42I CCGCGG 1 cut(s) 598
CseI GACGC 1 cut(s) 305
Csp6I GTAC 1 cut(s) 263
CviAII CATG 5 cut(s) 91, 232, 386, 574, 616
CviJI RGCY 8 cut(s) 245, 382, 485, 496, 502, 740, 760, 793
CviKI_1 RGCY 8 cut(s) 245, 382, 485, 496, 502, 740, 760, 793
CviQI GTAC 1 cut(s) 263
DdeI CTNAG 1 cut(s) 321
DpnI GATC 3 cut(s) 156, 665, 714
DpnII GATC 3 cut(s) 154, 663, 712
EaeI YGGCCR 1 cut(s) 243
EciI GGCGGA 1 cut(s) 130
Eco130I CCWWGG 2 cut(s) 231, 385
Eco24I GRGCYC 1 cut(s) 487
Eco31I GGTCTC 1 cut(s) 53
Eco32I GATATC 1 cut(s) 613
Eco47I GGWCC 2 cut(s) 533, 592
EcoRII CCWGG 3 cut(s) 497, 502, 529
EcoRV GATATC 1 cut(s) 613
EcoT14I CCWWGG 2 cut(s) 231, 385
EcoT38I GRGCYC 1 cut(s) 487
ErhI CCWWGG 2 cut(s) 231, 385
Esp3I CGTCTC 1 cut(s) 115
FaeI CATG 5 cut(s) 94, 235, 389, 577, 619
FalI AAGNNNNNCTT 2 cut(s) 285, 317
FaqI GGGAC 2 cut(s) 349, 763
FatI CATG 5 cut(s) 90, 231, 385, 573, 615
FauI CCCGC 2 cut(s) 111, 181
FauNDI CATATG 1 cut(s) 489
FbaI TGATCA 1 cut(s) 154
Fnu4HI GCNGC 3 cut(s) 17, 243, 421
FokI GGATG 1 cut(s) 520
FriOI GRGCYC 1 cut(s) 487
Fsp4HI GCNGC 3 cut(s) 17, 243, 421
GluI GCNGC 3 cut(s) 17, 243, 421
GsaI CCCAGC 1 cut(s) 540
HaeIII GGCC 3 cut(s) 245, 496, 502
HapII CCGG 3 cut(s) 80, 767, 800
HgaI GACGC 1 cut(s) 305
Hin1I GRCGYC 1 cut(s) 297
Hin1II CATG 5 cut(s) 94, 235, 389, 577, 619
HincII GTYRAC 1 cut(s) 220
HindII GTYRAC 1 cut(s) 220
HinfI GANTC 2 cut(s) 323, 457
HpaII CCGG 3 cut(s) 80, 767, 800
HphI GGTGA 3 cut(s) 220, 401, 475
Hpy166II GTNNAC 2 cut(s) 220, 228
Hpy188I TCNGA 6 cut(s) 123, 154, 256, 322, 571, 648
Hpy188III TCNNGA 3 cut(s) 40, 91, 800
Hpy8I GTNNAC 2 cut(s) 220, 228
HpyAV CCTTC 4 cut(s) 311, 556, 583, 691
HpyCH4IV ACGT 3 cut(s) 338, 650, 752
HpyCH4V TGCA 1 cut(s) 364
HpyF10VI GCNNNNNNNGC 1 cut(s) 16
HpyF3I CTNAG 1 cut(s) 321
HpySE526I ACGT 3 cut(s) 338, 650, 752
Hsp92I GRCGYC 1 cut(s) 297
Hsp92II CATG 5 cut(s) 94, 235, 389, 577, 619
Kpn2I TCCGGA 1 cut(s) 799
Ksp22I TGATCA 1 cut(s) 154
KspI CCGCGG 1 cut(s) 598
Kzo9I GATC 3 cut(s) 154, 663, 712
LmnI GCTCC 3 cut(s) 7, 737, 790
MaeII ACGT 3 cut(s) 338, 650, 752
MaeIII GTNAC 1 cut(s) 272
MalI GATC 3 cut(s) 156, 665, 714
MbiI CCGCTC 1 cut(s) 106
MboI GATC 3 cut(s) 154, 663, 712
MboII GAAGA 5 cut(s) 159, 305, 367, 462, 620
MflI RGATCY 1 cut(s) 712
MhlI GDGCHC 1 cut(s) 487
MluCI AATT 2 cut(s) 368, 689
MlyI GAGTC 1 cut(s) 332
MnlI CCTC 7 cut(s) 98, 148, 154, 208, 343, 486, 781
MroI TCCGGA 1 cut(s) 799
MroXI GAANNNNTTC 1 cut(s) 75
MseI TTAA 2 cut(s) 621, 653
MspA1I CMGCKG 2 cut(s) 176, 597
MspI CCGG 3 cut(s) 80, 767, 800
MspR9I CCNGG 3 cut(s) 499, 504, 531
MvaI CCWGG 3 cut(s) 499, 504, 531
MvnI CGCG 2 cut(s) 423, 597
MwoI GCNNNNNNNGC 1 cut(s) 16
NcoI CCATGG 2 cut(s) 231, 385
NdeI CATATG 1 cut(s) 489
NdeII GATC 3 cut(s) 154, 663, 712
NlaIII CATG 5 cut(s) 94, 235, 389, 577, 619
NlaIV GGNNCC 2 cut(s) 535, 792
NmeAIII GCCGAG 1 cut(s) 238
PagI TCATGA 1 cut(s) 90
PcsI WCGNNNNNNNCGW 1 cut(s) 335
PdmI GAANNNNTTC 1 cut(s) 75
PfeI GAWTC 1 cut(s) 457
PkrI GCNGC 3 cut(s) 18, 244, 422
PleI GAGTC 1 cut(s) 331
PpsI GAGTC 1 cut(s) 331
Psp6I CCWGG 3 cut(s) 497, 502, 529
PspFI CCCAGC 1 cut(s) 536
PspGI CCWGG 3 cut(s) 497, 502, 529
PspN4I GGNNCC 2 cut(s) 535, 792
PspPI GGNCC 3 cut(s) 495, 533, 592
PsuI RGATCY 1 cut(s) 712
RsaI GTAC 1 cut(s) 264
RsaNI GTAC 1 cut(s) 263
SacII CCGCGG 1 cut(s) 598
SaqAI TTAA 2 cut(s) 621, 653
SatI GCNGC 3 cut(s) 17, 243, 421
Sau3AI GATC 3 cut(s) 154, 663, 712
Sau96I GGNCC 3 cut(s) 495, 533, 592
SchI GAGTC 1 cut(s) 332
ScrFI CCNGG 3 cut(s) 499, 504, 531
SduI GDGCHC 1 cut(s) 487
SetI ASST 9 cut(s) 130, 219, 341, 399, 465, 653, 742, 755, 762
Sfr303I CCGCGG 1 cut(s) 598
SgrBI CCGCGG 1 cut(s) 598
SinI GGWCC 2 cut(s) 533, 592
SmlI CTYRAG 2 cut(s) 398, 707
SmoI CTYRAG 2 cut(s) 398, 707
Sse9I AATT 2 cut(s) 368, 689
SsiI CCGC 9 cut(s) 16, 104, 141, 147, 174, 242, 421, 595, 597
SspI AATATT 1 cut(s) 76
StyD4I CCNGG 3 cut(s) 497, 502, 529
StyI CCWWGG 2 cut(s) 231, 385
TaiI ACGT 3 cut(s) 341, 653, 755
TaqI TCGA 1 cut(s) 786
TasI AATT 2 cut(s) 368, 689
TauI GCSGC 3 cut(s) 19, 245, 423
TfiI GAWTC 1 cut(s) 457
Tru1I TTAA 2 cut(s) 621, 653
Tru9I TTAA 2 cut(s) 621, 653
TspDTI ATGAA 5 cut(s) 107, 392, 458, 482, 590
TspGWI ACGGA 2 cut(s) 301, 334
VpaK11BI GGWCC 2 cut(s) 533, 592
XapI RAATTY 1 cut(s) 368
XmnI GAANNNNTTC 1 cut(s) 75
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.