Rw1G028270

General transcription factor IIF subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0013034
Physical Location & Seq
Reverse (-)
55908339 .. 55911682
3344 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G028270.1

Sequence Viewer

Length: 678 bp
ATGGCGCCAAGCCAAATAGCAATGGAAGACCAAAAACCACAACGAACCAGTAGCACTAGTAATAGTGGTGCCAACAACAACAACAGCAACAAAAATTTGAGGGGGCATTTGGAGACGAAAAGCAAAGAAAATTGGGGGCATTTGGAGACGAGCAAAGCAGCGGAGCCAGTGTGGCTTATGAAATGCCCTCCCAACGTCGCCAAAGCGTTTAAGCCAGACGCCGCCGCCGACGGCGGCTCCGTCACCGCAGAGGAGCCTTGTCCTTCCGGCCCTGTCGCCAAGGTCATCCTCTCCCTTGACCCTCTCAACTCCAATAACGAACCTAAGTTTACCATGGAACTGGCGGCCACTGAATCTGCAAATACCCCCAAGTGTTACGTTTTGGATATGTCCAGGGACTTCATTCCAATGTCTGTGTTTTCAGAGTCGTCAGAAGGAAAGTTATCTGTGGAGGGGAAAATCTTCAACAAATTTGACATGAAGCCCCATGCTGAGAATCTTGAGAATTATGGGAAACTTTGCCGTGAACGAACAAAAAAGCATATGAAAAGTAGAAAGATTCAGGTGATCGACAATGACAATGGGGCACATATGAGGCCTATGCCAGGGATGATTGGTTTTGTGGTTCCTGGACCCAGTGTACATAGCTACCTACTTTTTGTGCTAATTCTTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000375 GO:0000377 GO:0000398 GO:0000428 GO:0000988 GO:0000990 GO:0000991 GO:0003674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0005674 GO:0005856 GO:0006139 GO:0006351 GO:0006352 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006367 GO:0006368 GO:0006370 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0008150 GO:0008152 GO:0008380 GO:0008543 GO:0009058 GO:0009059 GO:0009301 GO:0009452 GO:0009719 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0015630 GO:0016070 GO:0016071 GO:0016073 GO:0016591 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0023052 GO:0030880 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031334 GO:0031974 GO:0031981 GO:0032774 GO:0032784 GO:0032786 GO:0032968 GO:0032991 GO:0032993 GO:0034243 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0036260 GO:0042221 GO:0042795 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044344 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044798 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051716 GO:0055029 GO:0060255 GO:0060260 GO:0060261 GO:0061695 GO:0065007 GO:0070013 GO:0070848 GO:0070887 GO:0071310 GO:0071363 GO:0071495 GO:0071704 GO:0071774 GO:0080090 GO:0090304 GO:0090575 GO:0097550 GO:0097659 GO:0098781 GO:0140110 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1902680 GO:1903506 GO:1903508 GO:1990234 GO:2000112 GO:2000142 GO:2000144 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03022 map03022
Pfam Domains
Protein Families
TFIIF_beta

Protein Analysis

225

Amino Acids

24.72

Weight (kDa)

7.69

Isoelectric Point (pI)

34.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013034)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52270
fragaria_vesca FvH4_7g19881
malus_domestica MD01G1111000.v1.1 MD07G1177200.v1.1
prunus_persica Prupe.2G215900_v2.0.a1
pyrus_communis pycom07g17120
rosa_chinensis RchiOBHm_Chr1g0363871
rosa_laevigata RLG00000027539
rosa_multiflora Rmu_sc0002483.1_g000003
rosa_roxburghii Rroxscaffold_4G00292900
rosa_rugosa Rorug01G0310400 Rorug01G0310500 Rorug01G0310600
rosa_samantha Rh1AG318500 Rh1BG281700 Rh1CG298100 Rh1DG313000
rosa_wichuraiana Rw1G028270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 4, 68
AciI CCGC 6 cut(s) 161, 222, 225, 234, 246, 344
AcoI YGGCCR 1 cut(s) 345
AcsI RAATTY 2 cut(s) 94, 470
AcyI GRCGYC 2 cut(s) 5, 219
AfaI GTAC 1 cut(s) 642
AfiI CCNNNNNNNGG 1 cut(s) 605
AgsI TTSAA 1 cut(s) 466
AhlI ACTAGT 1 cut(s) 56
AjnI CCWGG 3 cut(s) 392, 604, 628
AluBI AGCT 1 cut(s) 648
AluI AGCT 1 cut(s) 648
Alw26I GTCTC 2 cut(s) 107, 140
AoxI GGCC 3 cut(s) 268, 345, 596
ApeKI GCWGC 1 cut(s) 158
ApoI RAATTY 2 cut(s) 94, 470
Asp700I GAANNNNTTC 1 cut(s) 461
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 2 cut(s) 269, 632
AsuHPI GGTGA 2 cut(s) 235, 577
AvaII GGWCC 1 cut(s) 632
BaeGI GKGCMC 1 cut(s) 589
BanI GGYRCC 2 cut(s) 4, 68
BbsI GAAGAC 1 cut(s) 33
BbvI GCAGC 1 cut(s) 170
BceAI ACGGC 2 cut(s) 247, 507
BciT130I CCWGG 3 cut(s) 394, 606, 630
BcoDI GTCTC 2 cut(s) 107, 140
BcuI ACTAGT 1 cut(s) 56
BfaI CTAG 1 cut(s) 57
BfoI RGCGCY 1 cut(s) 8
BglI GCCNNNNNGGC 1 cut(s) 172
BisI GCNGC 5 cut(s) 159, 222, 225, 235, 345
BlsI GCNGC 5 cut(s) 160, 223, 226, 236, 346
Bme1390I CCNGG 3 cut(s) 394, 606, 630
Bme18I GGWCC 1 cut(s) 632
BmgT120I GGNCC 2 cut(s) 269, 632
BmiI GGNNCC 7 cut(s) 6, 70, 165, 238, 255, 627, 634
BmrFI CCNGG 3 cut(s) 394, 606, 630
BmrI ACTGGG 1 cut(s) 630
BmuI ACTGGG 1 cut(s) 630
BpiI GAAGAC 1 cut(s) 33
BpuEI CTTGAG 1 cut(s) 521
BsaHI GRCGYC 2 cut(s) 5, 219
BsaJI CCNNGG 4 cut(s) 279, 333, 393, 605
BsaXI ACNNNNNCTCC 4 cut(s) 155, 185, 221, 251
Bsc4I CCNNNNNNNGG 1 cut(s) 605
Bse1I ACTGG 4 cut(s) 48, 167, 345, 636
Bse3DI GCAATG 1 cut(s) 27
BseBI CCWGG 3 cut(s) 394, 606, 630
BseDI CCNNGG 4 cut(s) 279, 333, 393, 605
BseGI GGATG 2 cut(s) 285, 615
BseLI CCNNNNNNNGG 1 cut(s) 605
BseMI GCAATG 1 cut(s) 27
BseMII CTCAG 1 cut(s) 483
BseNI ACTGG 4 cut(s) 48, 167, 345, 636
BseRI GAGGAG 1 cut(s) 266
BseSI GKGCMC 1 cut(s) 589
BseXI GCAGC 1 cut(s) 170
BshFI GGCC 3 cut(s) 270, 347, 598
BshNI GGYRCC 2 cut(s) 4, 68
BsiSI CCGG 1 cut(s) 267
BslFI GGGAC 1 cut(s) 410
BslI CCNNNNNNNGG 1 cut(s) 605
BsmAI GTCTC 2 cut(s) 107, 140
BsmBI CGTCTC 2 cut(s) 107, 140
BsmFI GGGAC 1 cut(s) 410
BsnI GGCC 3 cut(s) 270, 347, 598
Bsp1286I GDGCHC 1 cut(s) 589
Bsp1407I TGTACA 1 cut(s) 640
Bsp143I GATC 1 cut(s) 567
Bsp19I CCATGG 1 cut(s) 333
BspACI CCGC 6 cut(s) 161, 222, 225, 234, 246, 344
BspANI GGCC 3 cut(s) 270, 347, 598
BspCNI CTCAG 1 cut(s) 484
BspLI GGNNCC 7 cut(s) 6, 70, 165, 238, 255, 627, 634
BspT107I GGYRCC 2 cut(s) 4, 68
BsrDI GCAATG 1 cut(s) 27
BsrGI TGTACA 1 cut(s) 640
BsrI ACTGG 4 cut(s) 48, 167, 345, 636
BssECI CCNNGG 4 cut(s) 279, 333, 393, 605
BssMI GATC 1 cut(s) 567
BssNI GRCGYC 2 cut(s) 5, 219
BssT1I CCWWGG 2 cut(s) 279, 333
Bst2UI CCWGG 3 cut(s) 394, 606, 630
BstACI GRCGYC 2 cut(s) 5, 219
BstAUI TGTACA 1 cut(s) 640
BstDEI CTNAG 2 cut(s) 324, 492
BstDSI CCRYGG 1 cut(s) 333
BstENI CCTNNNNNAGG 1 cut(s) 603
BstF5I GGATG 2 cut(s) 285, 615
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 1 cut(s) 570
BstMAI GTCTC 2 cut(s) 107, 140
BstMBI GATC 1 cut(s) 567
BstMWI GCNNNNNNNGC 1 cut(s) 172
BstNI CCWGG 3 cut(s) 394, 606, 630
BstSCI CCNGG 3 cut(s) 392, 604, 628
BstSLI GKGCMC 1 cut(s) 589
BstV1I GCAGC 1 cut(s) 170
BstV2I GAAGAC 1 cut(s) 33
BstXI CCANNNNNNTGG 1 cut(s) 340
BsuRI GGCC 3 cut(s) 270, 347, 598
BtgI CCRYGG 1 cut(s) 333
BtsCI GGATG 2 cut(s) 285, 615
BtsIMutI CAGTG 3 cut(s) 174, 348, 643
CfoI GCGC 1 cut(s) 7
Cfr13I GGNCC 2 cut(s) 269, 632
CseI GACGC 1 cut(s) 227
Csp6I GTAC 1 cut(s) 641
CviAII CATG 3 cut(s) 334, 478, 488
CviQI GTAC 1 cut(s) 641
DdeI CTNAG 2 cut(s) 324, 492
DinI GGCGCC 1 cut(s) 6
DpnI GATC 1 cut(s) 569
DpnII GATC 1 cut(s) 567
EaeI YGGCCR 1 cut(s) 345
Eco130I CCWWGG 2 cut(s) 279, 333
Eco147I AGGCCT 1 cut(s) 598
Eco47I GGWCC 1 cut(s) 632
EcoNI CCTNNNNNAGG 1 cut(s) 603
EcoRII CCWGG 3 cut(s) 392, 604, 628
EcoT14I CCWWGG 2 cut(s) 279, 333
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 2 cut(s) 279, 333
Esp3I CGTCTC 2 cut(s) 107, 140
FaeI CATG 3 cut(s) 337, 481, 491
FaqI GGGAC 1 cut(s) 410
FatI CATG 3 cut(s) 333, 477, 487
FauNDI CATATG 2 cut(s) 543, 591
Fnu4HI GCNGC 5 cut(s) 159, 222, 225, 235, 345
FokI GGATG 2 cut(s) 272, 622
Fsp4HI GCNGC 5 cut(s) 159, 222, 225, 235, 345
FspBI CTAG 1 cut(s) 57
GlaI GCGC 1 cut(s) 6
GluI GCNGC 5 cut(s) 159, 222, 225, 235, 345
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 3 cut(s) 270, 347, 598
HapII CCGG 1 cut(s) 267
HgaI GACGC 1 cut(s) 227
HhaI GCGC 1 cut(s) 7
Hin1I GRCGYC 2 cut(s) 5, 219
Hin1II CATG 3 cut(s) 337, 481, 491
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HinfI GANTC 4 cut(s) 353, 425, 496, 559
HpaII CCGG 1 cut(s) 267
HphI GGTGA 2 cut(s) 235, 577
Hpy166II GTNNAC 3 cut(s) 330, 527, 641
Hpy188I TCNGA 2 cut(s) 424, 433
Hpy188III TCNNGA 2 cut(s) 500, 671
Hpy8I GTNNAC 3 cut(s) 330, 527, 641
Hpy99I CGWCG 2 cut(s) 200, 233
HpyAV CCTTC 2 cut(s) 273, 428
HpyCH4IV ACGT 2 cut(s) 195, 378
HpyCH4V TGCA 1 cut(s) 359
HpyF10VI GCNNNNNNNGC 1 cut(s) 172
HpyF3I CTNAG 2 cut(s) 324, 492
HpySE526I ACGT 2 cut(s) 195, 378
Hsp92I GRCGYC 2 cut(s) 5, 219
Hsp92II CATG 3 cut(s) 337, 481, 491
HspAI GCGC 1 cut(s) 5
KasI GGCGCC 1 cut(s) 4
Kzo9I GATC 1 cut(s) 567
LmnI GCTCC 3 cut(s) 163, 242, 253
Lsp1109I GCAGC 1 cut(s) 170
MaeI CTAG 1 cut(s) 57
MaeII ACGT 2 cut(s) 195, 378
MaeIII GTNAC 2 cut(s) 241, 374
MalI GATC 1 cut(s) 569
MboI GATC 1 cut(s) 567
MboII GAAGA 2 cut(s) 38, 454
MhlI GDGCHC 1 cut(s) 589
MluCI AATT 5 cut(s) 94, 130, 470, 505, 666
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 1 cut(s) 434
MnlI CCTC 7 cut(s) 93, 198, 244, 299, 312, 445, 588
MroXI GAANNNNTTC 1 cut(s) 461
MseI TTAA 1 cut(s) 210
MslI CAYNNNNRTG 1 cut(s) 407
MspA1I CMGCKG 1 cut(s) 161
MspI CCGG 1 cut(s) 267
MspR9I CCNGG 3 cut(s) 394, 606, 630
MvaI CCWGG 3 cut(s) 394, 606, 630
MwoI GCNNNNNNNGC 1 cut(s) 172
NarI GGCGCC 1 cut(s) 5
NcoI CCATGG 1 cut(s) 333
NdeI CATATG 2 cut(s) 543, 591
NdeII GATC 1 cut(s) 567
NlaIII CATG 3 cut(s) 337, 481, 491
NlaIV GGNNCC 7 cut(s) 6, 70, 165, 238, 255, 627, 634
NmuCI GTSAC 1 cut(s) 241
PceI AGGCCT 1 cut(s) 598
PcsI WCGNNNNNNNCGW 2 cut(s) 225, 237
PdmI GAANNNNTTC 1 cut(s) 461
PfeI GAWTC 3 cut(s) 353, 496, 559
PfoI TCCNGGA 1 cut(s) 628
PkrI GCNGC 5 cut(s) 160, 223, 226, 236, 346
PleI GAGTC 1 cut(s) 433
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 1 cut(s) 433
Psp6I CCWGG 3 cut(s) 392, 604, 628
PspGI CCWGG 3 cut(s) 392, 604, 628
PspN4I GGNNCC 7 cut(s) 6, 70, 165, 238, 255, 627, 634
PspPI GGNCC 2 cut(s) 269, 632
RsaI GTAC 1 cut(s) 642
RsaNI GTAC 1 cut(s) 641
RseI CAYNNNNRTG 1 cut(s) 407
SaqAI TTAA 1 cut(s) 210
SatI GCNGC 5 cut(s) 159, 222, 225, 235, 345
Sau3AI GATC 1 cut(s) 567
Sau96I GGNCC 2 cut(s) 269, 632
SchI GAGTC 1 cut(s) 434
ScrFI CCNGG 3 cut(s) 394, 606, 630
SduI GDGCHC 1 cut(s) 589
SetI ASST 7 cut(s) 198, 285, 325, 381, 567, 650, 654
SfoI GGCGCC 1 cut(s) 6
SinI GGWCC 1 cut(s) 632
SmiMI CAYNNNNRTG 1 cut(s) 407
SmlI CTYRAG 2 cut(s) 500, 671
SmoI CTYRAG 2 cut(s) 500, 671
SpeI ACTAGT 1 cut(s) 56
Sse9I AATT 5 cut(s) 94, 130, 470, 505, 666
SseBI AGGCCT 1 cut(s) 598
SsiI CCGC 6 cut(s) 161, 222, 225, 234, 246, 344
SspDI GGCGCC 1 cut(s) 4
SspMI CTAG 1 cut(s) 57
StuI AGGCCT 1 cut(s) 598
StyD4I CCNGG 3 cut(s) 392, 604, 628
StyI CCWWGG 2 cut(s) 279, 333
TaiI ACGT 2 cut(s) 198, 381
TaqI TCGA 1 cut(s) 570
TasI AATT 5 cut(s) 94, 130, 470, 505, 666
TatI WGTACW 1 cut(s) 640
TauI GCSGC 4 cut(s) 224, 227, 237, 347
TfiI GAWTC 3 cut(s) 353, 496, 559
Tru1I TTAA 1 cut(s) 210
Tru9I TTAA 1 cut(s) 210
TscAI CASTG 3 cut(s) 174, 355, 643
TseFI GTSAC 1 cut(s) 241
TseI GCWGC 1 cut(s) 158
Tsp45I GTSAC 1 cut(s) 241
TspDTI ATGAA 4 cut(s) 194, 391, 494, 560
TspGWI ACGGA 1 cut(s) 229
TspRI CASTG 3 cut(s) 174, 355, 643
VpaK11BI GGWCC 1 cut(s) 632
XagI CCTNNNNNAGG 1 cut(s) 603
XapI RAATTY 2 cut(s) 94, 470
XmnI GAANNNNTTC 1 cut(s) 461
XspI CTAG 1 cut(s) 57
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.