MD01G1111000.v1.1

General transcription factor IIF subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0013034
Physical Location & Seq
Reverse (-)
22361673 .. 22364523
2851 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1111000.v1.1.491

Sequence Viewer

Length: 855 bp
ATGAACCCTATGGCCGTGGCGACAACCCAATGCCAAAAGATGGGAGACCAAAAGCCACAACAAGGCAATAAGAATGTCGTCAATGGGCGTTTGTTGGAGACCAAGGGAGCAAATATTCCACTGTGGCTCATGAAAACTCCCCCGCGCGTCTCTCGCTGTCTGAAACCTGATTTCTCAGCCGCCGCCGCTGCCTCCGCTTCTGATCAGCAGCAGTCTTCTCCCCCTGTGGCCAAGGTGGTTTTCTGTTTGGACCCTATCACCAAGACAACAGAGTTCACCATGGAACTGGCGGCCACGGAATCTGAAAATGTACCCAAGTGTTACGCTTTGGAGATGAGTAAAAACAAAATTCCAATGTCTGTGTTTGCGGAGTCATCACAAGGGACTTTATCCGTTGAGGGCTACATATTGGACAAACTTGACATGAAACCCCATAACGAGAACCTTGAGAACTATGGGAAACTTTGCCGCGAACGGACCAAAAAATCTATGAAGAGTAGAAAGATCCAGGTGATGAACAATGACAATGGGGCTCATATGAGACCCAGGCCTGGGATGATGATCGGTTTTGTGTCTCCTGGACCCAGCGAGAAAAAGAAAATGCCAACGAAGGGGTCAGACATGAAAAGAACAAGGAAGGACCGCGGAGAAATGGAGGATATTATGTTTAATCTCTTTGAACAGAAACCAAACCGGACGTTAAGGCAACTGATCCAAGAGACAAACCAACCAGAACAATTTTTGAAAGACATACTCAAGGACCTCTGTGTGTACAACAACAAGGGAGCTAATCAAGGGACGTATGAGCTGAAACCGGAATACAGGAAATCCGTCGAGGAGCCAACTCCCGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000375 GO:0000377 GO:0000398 GO:0000428 GO:0000988 GO:0000990 GO:0000991 GO:0003674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0005674 GO:0005856 GO:0006139 GO:0006351 GO:0006352 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006367 GO:0006368 GO:0006370 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0008150 GO:0008152 GO:0008380 GO:0008543 GO:0009058 GO:0009059 GO:0009301 GO:0009452 GO:0009719 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0015630 GO:0016070 GO:0016071 GO:0016073 GO:0016591 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0023052 GO:0030880 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031334 GO:0031974 GO:0031981 GO:0032774 GO:0032784 GO:0032786 GO:0032968 GO:0032991 GO:0032993 GO:0034243 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0036260 GO:0042221 GO:0042795 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044344 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044798 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051716 GO:0055029 GO:0060255 GO:0060260 GO:0060261 GO:0061695 GO:0065007 GO:0070013 GO:0070848 GO:0070887 GO:0071310 GO:0071363 GO:0071495 GO:0071704 GO:0071774 GO:0080090 GO:0090304 GO:0090575 GO:0097550 GO:0097659 GO:0098781 GO:0140110 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1902680 GO:1903506 GO:1903508 GO:1990234 GO:2000112 GO:2000142 GO:2000144 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03022 map03022
Pfam Domains
Protein Families
TFIIF_beta

Protein Analysis

285

Amino Acids

32.03

Weight (kDa)

9.23

Isoelectric Point (pI)

39.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TFIIF_beta PF02270 212 - 275 1.5e-16 TFIIF, beta subunit HTH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013034)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52270
fragaria_vesca FvH4_7g19881
malus_domestica MD01G1111000.v1.1 MD07G1177200.v1.1
prunus_persica Prupe.2G215900_v2.0.a1
pyrus_communis pycom07g17120
rosa_chinensis RchiOBHm_Chr1g0363871
rosa_laevigata RLG00000027539
rosa_multiflora Rmu_sc0002483.1_g000003
rosa_roxburghii Rroxscaffold_4G00292900
rosa_rugosa Rorug01G0310400 Rorug01G0310500 Rorug01G0310600
rosa_samantha Rh1AG318500 Rh1BG281700 Rh1CG298100 Rh1DG313000
rosa_wichuraiana Rw1G028270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 40
AccII CGCG 4 cut(s) 145, 147, 471, 645
AclWI GGATC 2 cut(s) 499, 706
AcoI YGGCCR 3 cut(s) 12, 228, 291
AcsI RAATTY 1 cut(s) 348
AfaI GTAC 2 cut(s) 312, 773
AfiI CCNNNNNNNGG 5 cut(s) 40, 62, 551, 552, 611
AgsI TTSAA 2 cut(s) 680, 745
AjnI CCWGG 4 cut(s) 507, 545, 550, 577
AjuI GAANNNNNNNTTGG 2 cut(s) 224, 256
AluBI AGCT 2 cut(s) 788, 808
AluI AGCT 2 cut(s) 788, 808
Alw26I GTCTC 6 cut(s) 39, 92, 154, 535, 579, 713
AlwI GGATC 2 cut(s) 499, 706
AoxI GGCC 4 cut(s) 12, 228, 291, 548
ApeKI GCWGC 2 cut(s) 188, 208
ApoI RAATTY 1 cut(s) 348
AspLEI GCGC 1 cut(s) 147
AspS9I GGNCC 5 cut(s) 250, 477, 581, 640, 760
AsuHPI GGTGA 3 cut(s) 250, 268, 523
AvaII GGWCC 5 cut(s) 250, 477, 581, 640, 760
BalI TGGCCA 1 cut(s) 230
BanII GRGCYC 1 cut(s) 535
BbsI GAAGAC 1 cut(s) 207
BbvI GCAGC 2 cut(s) 175, 220
BccI CCATC 1 cut(s) 34
BciT130I CCWGG 4 cut(s) 509, 547, 552, 579
BclI TGATCA 1 cut(s) 202
BcoDI GTCTC 6 cut(s) 39, 92, 154, 535, 579, 713
BisI GCNGC 7 cut(s) 180, 183, 186, 189, 209, 291, 469
BlsI GCNGC 7 cut(s) 181, 184, 187, 190, 210, 292, 470
Bme1390I CCNGG 4 cut(s) 509, 547, 552, 579
Bme18I GGWCC 5 cut(s) 250, 477, 581, 640, 760
BmgT120I GGNCC 5 cut(s) 250, 477, 581, 640, 760
BmiI GGNNCC 3 cut(s) 252, 583, 840
BmrFI CCNGG 4 cut(s) 509, 547, 552, 579
BpiI GAAGAC 1 cut(s) 207
BpuEI CTTGAG 2 cut(s) 467, 740
BsaBI GATNNNNATC 1 cut(s) 560
BsaI GGTCTC 3 cut(s) 39, 92, 535
BsaJI CCNNGG 8 cut(s) 15, 102, 231, 279, 294, 545, 551, 643
BsaWI WCCGGW 2 cut(s) 693, 814
Bsc4I CCNNNNNNNGG 5 cut(s) 40, 62, 551, 552, 611
Bse1I ACTGG 1 cut(s) 291
Bse8I GATNNNNATC 1 cut(s) 560
BseBI CCWGG 4 cut(s) 509, 547, 552, 579
BseDI CCNNGG 8 cut(s) 15, 102, 231, 279, 294, 545, 551, 643
BseGI GGATG 1 cut(s) 561
BseJI GATNNNNATC 1 cut(s) 560
BseLI CCNNNNNNNGG 5 cut(s) 40, 62, 551, 552, 611
BseMII CTCAG 1 cut(s) 189
BseNI ACTGG 1 cut(s) 291
BseRI GAGGAG 1 cut(s) 851
BseXI GCAGC 2 cut(s) 175, 220
BseYI CCCAGC 1 cut(s) 584
Bsh1236I CGCG 4 cut(s) 145, 147, 471, 645
BshFI GGCC 4 cut(s) 14, 230, 293, 550
BsiSI CCGG 2 cut(s) 694, 815
BslFI GGGAC 2 cut(s) 397, 811
BslI CCNNNNNNNGG 5 cut(s) 40, 62, 551, 552, 611
BsmAI GTCTC 6 cut(s) 39, 92, 154, 535, 579, 713
BsmBI CGTCTC 1 cut(s) 154
BsmFI GGGAC 2 cut(s) 397, 811
BsnI GGCC 4 cut(s) 14, 230, 293, 550
Bso31I GGTCTC 3 cut(s) 39, 92, 535
Bsp1286I GDGCHC 1 cut(s) 535
Bsp1407I TGTACA 1 cut(s) 771
Bsp143I GATC 4 cut(s) 202, 504, 561, 711
Bsp19I CCATGG 1 cut(s) 279
BspANI GGCC 4 cut(s) 14, 230, 293, 550
BspCNI CTCAG 1 cut(s) 188
BspFNI CGCG 4 cut(s) 145, 147, 471, 645
BspHI TCATGA 1 cut(s) 129
BspLI GGNNCC 3 cut(s) 252, 583, 840
BspPI GGATC 2 cut(s) 499, 706
BspTNI GGTCTC 3 cut(s) 39, 92, 535
BsrGI TGTACA 1 cut(s) 771
BsrI ACTGG 1 cut(s) 291
BssECI CCNNGG 8 cut(s) 15, 102, 231, 279, 294, 545, 551, 643
BssMI GATC 4 cut(s) 202, 504, 561, 711
BssT1I CCWWGG 3 cut(s) 102, 231, 279
Bst2UI CCWGG 4 cut(s) 509, 547, 552, 579
Bst4CI ACNGT 1 cut(s) 123
Bst6I CTCTTC 1 cut(s) 488
BstAUI TGTACA 1 cut(s) 771
BstDEI CTNAG 1 cut(s) 175
BstDSI CCRYGG 4 cut(s) 15, 279, 294, 643
BstF5I GGATG 1 cut(s) 561
BstFNI CGCG 4 cut(s) 145, 147, 471, 645
BstHHI GCGC 1 cut(s) 147
BstKTI GATC 4 cut(s) 205, 507, 564, 714
BstMAI GTCTC 6 cut(s) 39, 92, 154, 535, 579, 713
BstMBI GATC 4 cut(s) 202, 504, 561, 711
BstMWI GCNNNNNNNGC 4 cut(s) 153, 185, 188, 194
BstNI CCWGG 4 cut(s) 509, 547, 552, 579
BstSCI CCNGG 4 cut(s) 507, 545, 550, 577
BstUI CGCG 4 cut(s) 145, 147, 471, 645
BstV1I GCAGC 2 cut(s) 175, 220
BstV2I GAAGAC 1 cut(s) 207
BstX2I RGATCY 1 cut(s) 504
BstXI CCANNNNNNTGG 1 cut(s) 286
BstYI RGATCY 1 cut(s) 504
BsuRI GGCC 4 cut(s) 14, 230, 293, 550
BtgI CCRYGG 4 cut(s) 15, 279, 294, 643
BtsCI GGATG 1 cut(s) 561
BtsIMutI CAGTG 1 cut(s) 119
CciI TCATGA 1 cut(s) 129
CfoI GCGC 1 cut(s) 147
Cfr13I GGNCC 5 cut(s) 250, 477, 581, 640, 760
Cfr42I CCGCGG 1 cut(s) 646
CseI GACGC 1 cut(s) 136
Csp6I GTAC 2 cut(s) 311, 772
CviAII CATG 4 cut(s) 130, 280, 424, 622
CviQI GTAC 2 cut(s) 311, 772
DdeI CTNAG 1 cut(s) 175
DpnI GATC 4 cut(s) 204, 506, 563, 713
DpnII GATC 4 cut(s) 202, 504, 561, 711
EaeI YGGCCR 3 cut(s) 12, 228, 291
Eam1104I CTCTTC 1 cut(s) 488
EarI CTCTTC 1 cut(s) 488
Eco130I CCWWGG 3 cut(s) 102, 231, 279
Eco147I AGGCCT 1 cut(s) 550
Eco24I GRGCYC 1 cut(s) 535
Eco31I GGTCTC 3 cut(s) 39, 92, 535
Eco47I GGWCC 5 cut(s) 250, 477, 581, 640, 760
EcoO109I RGGNCCY 1 cut(s) 760
EcoRII CCWGG 4 cut(s) 507, 545, 550, 577
EcoT14I CCWWGG 3 cut(s) 102, 231, 279
EcoT38I GRGCYC 1 cut(s) 535
ErhI CCWWGG 3 cut(s) 102, 231, 279
Esp3I CGTCTC 1 cut(s) 154
FaeI CATG 4 cut(s) 133, 283, 427, 625
FaqI GGGAC 2 cut(s) 397, 811
FatI CATG 4 cut(s) 129, 279, 423, 621
FauI CCCGC 1 cut(s) 150
FauNDI CATATG 1 cut(s) 537
FbaI TGATCA 1 cut(s) 202
Fnu4HI GCNGC 7 cut(s) 180, 183, 186, 189, 209, 291, 469
FokI GGATG 1 cut(s) 568
FriOI GRGCYC 1 cut(s) 535
Fsp4HI GCNGC 7 cut(s) 180, 183, 186, 189, 209, 291, 469
GlaI GCGC 1 cut(s) 146
GluI GCNGC 7 cut(s) 180, 183, 186, 189, 209, 291, 469
GsaI CCCAGC 1 cut(s) 588
HaeIII GGCC 4 cut(s) 14, 230, 293, 550
HapII CCGG 2 cut(s) 694, 815
HgaI GACGC 1 cut(s) 136
HhaI GCGC 1 cut(s) 147
Hin1II CATG 4 cut(s) 133, 283, 427, 625
Hin6I GCGC 1 cut(s) 145
HinP1I GCGC 1 cut(s) 145
HinfI GANTC 2 cut(s) 299, 371
HpaII CCGG 2 cut(s) 694, 815
HphI GGTGA 3 cut(s) 250, 268, 523
Hpy166II GTNNAC 2 cut(s) 276, 772
Hpy188I TCNGA 4 cut(s) 162, 202, 304, 619
Hpy188III TCNNGA 2 cut(s) 130, 848
Hpy8I GTNNAC 2 cut(s) 276, 772
Hpy99I CGWCG 1 cut(s) 836
HpyAV CCTTC 2 cut(s) 604, 631
HpyCH4III ACNGT 1 cut(s) 123
HpyCH4IV ACGT 2 cut(s) 698, 800
HpyF10VI GCNNNNNNNGC 4 cut(s) 153, 185, 188, 194
HpyF3I CTNAG 1 cut(s) 175
HpySE526I ACGT 2 cut(s) 698, 800
Hsp92II CATG 4 cut(s) 133, 283, 427, 625
HspAI GCGC 1 cut(s) 145
Ksp22I TGATCA 1 cut(s) 202
KspI CCGCGG 1 cut(s) 646
Kzo9I GATC 4 cut(s) 202, 504, 561, 711
LmnI GCTCC 3 cut(s) 107, 785, 838
Lsp1109I GCAGC 2 cut(s) 175, 220
MaeII ACGT 2 cut(s) 698, 800
MaeIII GTNAC 1 cut(s) 320
MalI GATC 4 cut(s) 204, 506, 563, 713
MboI GATC 4 cut(s) 202, 504, 561, 711
MboII GAAGA 2 cut(s) 207, 505
MflI RGATCY 1 cut(s) 504
MhlI GDGCHC 1 cut(s) 535
MlsI TGGCCA 1 cut(s) 230
MluCI AATT 2 cut(s) 348, 737
MluNI TGGCCA 1 cut(s) 230
MlyI GAGTC 1 cut(s) 380
MmeI TCCRAC 1 cut(s) 75
MnlI CCTC 5 cut(s) 202, 391, 649, 773, 829
Mox20I TGGCCA 1 cut(s) 230
MscI TGGCCA 1 cut(s) 230
MseI TTAA 2 cut(s) 669, 701
Msp20I TGGCCA 1 cut(s) 230
MspA1I CMGCKG 2 cut(s) 188, 645
MspI CCGG 2 cut(s) 694, 815
MspR9I CCNGG 4 cut(s) 509, 547, 552, 579
MvaI CCWGG 4 cut(s) 509, 547, 552, 579
MvnI CGCG 4 cut(s) 145, 147, 471, 645
MwoI GCNNNNNNNGC 4 cut(s) 153, 185, 188, 194
NcoI CCATGG 1 cut(s) 279
NdeI CATATG 1 cut(s) 537
NdeII GATC 4 cut(s) 202, 504, 561, 711
NlaIII CATG 4 cut(s) 133, 283, 427, 625
NlaIV GGNNCC 3 cut(s) 252, 583, 840
PagI TCATGA 1 cut(s) 129
PceI AGGCCT 1 cut(s) 550
PfeI GAWTC 1 cut(s) 299
PflMI CCANNNNNTGG 1 cut(s) 40
PfoI TCCNGGA 1 cut(s) 577
PkrI GCNGC 7 cut(s) 181, 184, 187, 190, 210, 292, 470
PleI GAGTC 1 cut(s) 379
PpsI GAGTC 1 cut(s) 379
PpuMI RGGWCCY 1 cut(s) 760
Psp5II RGGWCCY 1 cut(s) 760
Psp6I CCWGG 4 cut(s) 507, 545, 550, 577
PspFI CCCAGC 1 cut(s) 584
PspGI CCWGG 4 cut(s) 507, 545, 550, 577
PspN4I GGNNCC 3 cut(s) 252, 583, 840
PspPI GGNCC 5 cut(s) 250, 477, 581, 640, 760
PspPPI RGGWCCY 1 cut(s) 760
PsuI RGATCY 1 cut(s) 504
RsaI GTAC 2 cut(s) 312, 773
RsaNI GTAC 2 cut(s) 311, 772
SacII CCGCGG 1 cut(s) 646
SaqAI TTAA 2 cut(s) 669, 701
SatI GCNGC 7 cut(s) 180, 183, 186, 189, 209, 291, 469
Sau3AI GATC 4 cut(s) 202, 504, 561, 711
Sau96I GGNCC 5 cut(s) 250, 477, 581, 640, 760
SchI GAGTC 1 cut(s) 380
ScrFI CCNGG 4 cut(s) 509, 547, 552, 579
SduI GDGCHC 1 cut(s) 535
SetI ASST 9 cut(s) 169, 237, 447, 513, 701, 765, 790, 803, 810
Sfr303I CCGCGG 1 cut(s) 646
SgrBI CCGCGG 1 cut(s) 646
SinI GGWCC 5 cut(s) 250, 477, 581, 640, 760
SmlI CTYRAG 2 cut(s) 446, 755
SmoI CTYRAG 2 cut(s) 446, 755
Sse9I AATT 2 cut(s) 348, 737
SseBI AGGCCT 1 cut(s) 550
SspI AATATT 1 cut(s) 115
StuI AGGCCT 1 cut(s) 550
StyD4I CCNGG 4 cut(s) 507, 545, 550, 577
StyI CCWWGG 3 cut(s) 102, 231, 279
TaaI ACNGT 1 cut(s) 123
TaiI ACGT 2 cut(s) 701, 803
TaqI TCGA 1 cut(s) 834
TasI AATT 2 cut(s) 348, 737
TatI WGTACW 1 cut(s) 771
TauI GCSGC 5 cut(s) 182, 185, 188, 293, 471
TfiI GAWTC 1 cut(s) 299
Tru1I TTAA 2 cut(s) 669, 701
Tru9I TTAA 2 cut(s) 669, 701
TscAI CASTG 1 cut(s) 126
TseI GCWGC 2 cut(s) 188, 208
TspDTI ATGAA 6 cut(s) 17, 146, 440, 506, 530, 638
TspGWI ACGGA 4 cut(s) 311, 382, 490, 820
TspRI CASTG 1 cut(s) 126
Van91I CCANNNNNTGG 1 cut(s) 40
VpaK11BI GGWCC 5 cut(s) 250, 477, 581, 640, 760
XapI RAATTY 1 cut(s) 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.