MD07G1177200.v1.1

General transcription factor IIF subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0013034
Physical Location & Seq
Reverse (-)
25645654 .. 25648333
2680 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1177200.v1.1.491

Sequence Viewer

Length: 834 bp
ATGATGGAGGACCAAAAGCGGCAACAAGGCAATAACAATGTCGTGAATGGGCGTTATTTGGAGACCAACAGAGCAAATATGCCGGTGTTCCTCATGAAATCTCCCCCGCTCGTCTCTCGCTGTCTGAAACCTGATGTCTCCTCCGCCTCCGCTTCTGATCAGCAGCAGTCTTCTCCCGCTGTTGCCAAGTTGGTTCTCTCTTTTGACCCTATTGCCGAGGTCAGCGAGTTCACCATGGAACTGGCTGCCACGAAATCTGAAAATGTACCCAAGTGTTACGCTATGCATGTGTTTGAAGACGTTCTTCCAATGTCCGTGTTTTCTGAGTCATCGGAAGGGACGTTATCCGTGGAGGGGAGGATATTGCAGAAATTTGATATGAAGCCCCATGGTGAGAACCTTGAGAACTATGGGAAACTTTGCCGCGAAAGGACCAAAAAATATATGAAGAGTAGAAAGATTCAGGTGATGAACAACGACAATGGGGCTCATATGAGGCCCAGGCCAGGGATGATGATTGGTTTCATGTCTCCTGGAGCAAGCGAGAAAAAGAAAATGCCAACGAAGGGGTCAGACATGAAAAGAACAAGGAAGGACCGCGGAGAAATGGAAGATATCATGTTTAATCTCTTTGAACAGAAACCAAATCGGACGTTAAGGCAACTGATCCAAGAGACTAACCAACCAGAACAATTTTTGAAAGACATACTCAAGGATCTTTGTATCTACAACAACAAGGGAGCTAATCAAGGGACGTATGAGCTGAAACCGGAATACAGGAAATCAAACCCGAATGTGTTCTTCAATCTTCGGTATGAGCAAGACGAAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000375 GO:0000377 GO:0000398 GO:0000428 GO:0000988 GO:0000990 GO:0000991 GO:0003674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0005674 GO:0005856 GO:0006139 GO:0006351 GO:0006352 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006367 GO:0006368 GO:0006370 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0008150 GO:0008152 GO:0008380 GO:0008543 GO:0009058 GO:0009059 GO:0009301 GO:0009452 GO:0009719 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0015630 GO:0016070 GO:0016071 GO:0016073 GO:0016591 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0023052 GO:0030880 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031334 GO:0031974 GO:0031981 GO:0032774 GO:0032784 GO:0032786 GO:0032968 GO:0032991 GO:0032993 GO:0034243 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0036260 GO:0042221 GO:0042795 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044344 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044798 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051716 GO:0055029 GO:0060255 GO:0060260 GO:0060261 GO:0061695 GO:0065007 GO:0070013 GO:0070848 GO:0070887 GO:0071310 GO:0071363 GO:0071495 GO:0071704 GO:0071774 GO:0080090 GO:0090304 GO:0090575 GO:0097550 GO:0097659 GO:0098781 GO:0140110 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1902680 GO:1903506 GO:1903508 GO:1990234 GO:2000112 GO:2000142 GO:2000144 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03022 map03022
Pfam Domains
Protein Families
TFIIF_beta

Protein Analysis

278

Amino Acids

31.83

Weight (kDa)

9.0

Isoelectric Point (pI)

46.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TFIIF_beta PF02270 197 - 260 2.3e-16 TFIIF, beta subunit HTH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013034)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52270
fragaria_vesca FvH4_7g19881
malus_domestica MD01G1111000.v1.1 MD07G1177200.v1.1
prunus_persica Prupe.2G215900_v2.0.a1
pyrus_communis pycom07g17120
rosa_chinensis RchiOBHm_Chr1g0363871
rosa_laevigata RLG00000027539
rosa_multiflora Rmu_sc0002483.1_g000003
rosa_roxburghii Rroxscaffold_4G00292900
rosa_rugosa Rorug01G0310400 Rorug01G0310500 Rorug01G0310600
rosa_samantha Rh1AG318500 Rh1BG281700 Rh1CG298100 Rh1DG313000
rosa_wichuraiana Rw1G028270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 109
AccII CGCG 2 cut(s) 426, 600
AciI CCGC 8 cut(s) 19, 107, 144, 150, 177, 424, 598, 600
AclWI GGATC 2 cut(s) 661, 723
AcsI RAATTY 1 cut(s) 371
AfaI GTAC 1 cut(s) 267
AfiI CCNNNNNNNGG 4 cut(s) 354, 506, 507, 566
AgsI TTSAA 4 cut(s) 296, 635, 700, 805
AjnI CCWGG 3 cut(s) 500, 505, 532
AluBI AGCT 2 cut(s) 743, 763
AluI AGCT 2 cut(s) 743, 763
Alw26I GTCTC 5 cut(s) 56, 118, 142, 534, 668
AlwI GGATC 2 cut(s) 661, 723
AoxI GGCC 2 cut(s) 497, 503
ApeKI GCWGC 2 cut(s) 163, 245
ApoI RAATTY 1 cut(s) 371
Asp700I GAANNNNTTC 2 cut(s) 300, 797
AspS9I GGNCC 4 cut(s) 10, 432, 498, 595
AsuHPI GGTGA 3 cut(s) 223, 404, 478
AvaII GGWCC 3 cut(s) 10, 432, 595
BanII GRGCYC 1 cut(s) 490
BbsI GAAGAC 2 cut(s) 162, 303
BbvI GCAGC 2 cut(s) 175, 232
BciT130I CCWGG 3 cut(s) 502, 507, 534
BclI TGATCA 1 cut(s) 157
BcoDI GTCTC 5 cut(s) 56, 118, 142, 534, 668
BisI GCNGC 4 cut(s) 20, 164, 246, 424
BlsI GCNGC 4 cut(s) 21, 165, 247, 425
Bme1390I CCNGG 3 cut(s) 502, 507, 534
Bme18I GGWCC 3 cut(s) 10, 432, 595
BmgT120I GGNCC 4 cut(s) 10, 432, 498, 595
BmrFI CCNGG 3 cut(s) 502, 507, 534
BpiI GAAGAC 2 cut(s) 162, 303
BpmI CTGGAG 1 cut(s) 555
BpuEI CTTGAG 2 cut(s) 422, 695
BsaI GGTCTC 1 cut(s) 56
BsaJI CCNNGG 7 cut(s) 216, 234, 348, 388, 500, 506, 598
BsaWI WCCGGW 1 cut(s) 769
Bsc4I CCNNNNNNNGG 4 cut(s) 354, 506, 507, 566
Bse118I RCCGGY 1 cut(s) 82
Bse1I ACTGG 1 cut(s) 246
BseBI CCWGG 3 cut(s) 502, 507, 534
BseDI CCNNGG 7 cut(s) 216, 234, 348, 388, 500, 506, 598
BseGI GGATG 1 cut(s) 516
BseLI CCNNNNNNNGG 4 cut(s) 354, 506, 507, 566
BseMII CTCAG 1 cut(s) 315
BseNI ACTGG 1 cut(s) 246
BseRI GAGGAG 1 cut(s) 130
BseXI GCAGC 2 cut(s) 175, 232
Bsh1236I CGCG 2 cut(s) 426, 600
BshFI GGCC 2 cut(s) 499, 505
BsiSI CCGG 2 cut(s) 83, 770
BslFI GGGAC 2 cut(s) 352, 766
BslI CCNNNNNNNGG 4 cut(s) 354, 506, 507, 566
BsmAI GTCTC 5 cut(s) 56, 118, 142, 534, 668
BsmBI CGTCTC 1 cut(s) 118
BsmFI GGGAC 2 cut(s) 352, 766
BsnI GGCC 2 cut(s) 499, 505
Bso31I GGTCTC 1 cut(s) 56
Bsp1286I GDGCHC 1 cut(s) 490
Bsp143I GATC 3 cut(s) 157, 666, 715
Bsp19I CCATGG 2 cut(s) 234, 388
BspACI CCGC 8 cut(s) 19, 107, 144, 150, 177, 424, 598, 600
BspANI GGCC 2 cut(s) 499, 505
BspCNI CTCAG 1 cut(s) 316
BspFNI CGCG 2 cut(s) 426, 600
BspHI TCATGA 1 cut(s) 93
BspPI GGATC 2 cut(s) 661, 723
BspTNI GGTCTC 1 cut(s) 56
BsrBI CCGCTC 1 cut(s) 109
BsrFI RCCGGY 1 cut(s) 82
BsrI ACTGG 1 cut(s) 246
BssAI RCCGGY 1 cut(s) 82
BssECI CCNNGG 7 cut(s) 216, 234, 348, 388, 500, 506, 598
BssMI GATC 3 cut(s) 157, 666, 715
BssT1I CCWWGG 2 cut(s) 234, 388
Bst2UI CCWGG 3 cut(s) 502, 507, 534
Bst6I CTCTTC 1 cut(s) 443
BstC8I GCNNGC 1 cut(s) 541
BstDEI CTNAG 1 cut(s) 324
BstDSI CCRYGG 4 cut(s) 234, 348, 388, 598
BstF5I GGATG 1 cut(s) 516
BstFNI CGCG 2 cut(s) 426, 600
BstKTI GATC 3 cut(s) 160, 669, 718
BstMAI GTCTC 5 cut(s) 56, 118, 142, 534, 668
BstMBI GATC 3 cut(s) 157, 666, 715
BstNI CCWGG 3 cut(s) 502, 507, 534
BstNSI RCATGY 1 cut(s) 290
BstSCI CCNGG 3 cut(s) 500, 505, 532
BstUI CGCG 2 cut(s) 426, 600
BstV1I GCAGC 2 cut(s) 175, 232
BstV2I GAAGAC 2 cut(s) 162, 303
BstX2I RGATCY 1 cut(s) 715
BstXI CCANNNNNNTGG 1 cut(s) 241
BstYI RGATCY 1 cut(s) 715
BsuRI GGCC 2 cut(s) 499, 505
BtgI CCRYGG 4 cut(s) 234, 348, 388, 598
BtsCI GGATG 1 cut(s) 516
Cac8I GCNNGC 1 cut(s) 541
CciI TCATGA 1 cut(s) 93
Cfr10I RCCGGY 1 cut(s) 82
Cfr13I GGNCC 4 cut(s) 10, 432, 498, 595
Cfr42I CCGCGG 1 cut(s) 601
Csp6I GTAC 1 cut(s) 266
CviAII CATG 7 cut(s) 94, 235, 287, 389, 526, 577, 619
CviJI RGCY 7 cut(s) 245, 385, 488, 499, 505, 743, 763
CviKI_1 RGCY 7 cut(s) 245, 385, 488, 499, 505, 743, 763
CviQI GTAC 1 cut(s) 266
DdeI CTNAG 1 cut(s) 324
DpnI GATC 3 cut(s) 159, 668, 717
DpnII GATC 3 cut(s) 157, 666, 715
Eam1104I CTCTTC 1 cut(s) 443
EarI CTCTTC 1 cut(s) 443
EciI GGCGGA 1 cut(s) 133
Eco130I CCWWGG 2 cut(s) 234, 388
Eco24I GRGCYC 1 cut(s) 490
Eco31I GGTCTC 1 cut(s) 56
Eco32I GATATC 1 cut(s) 616
Eco47I GGWCC 3 cut(s) 10, 432, 595
EcoRII CCWGG 3 cut(s) 500, 505, 532
EcoRV GATATC 1 cut(s) 616
EcoT14I CCWWGG 2 cut(s) 234, 388
EcoT22I ATGCAT 1 cut(s) 288
EcoT38I GRGCYC 1 cut(s) 490
ErhI CCWWGG 2 cut(s) 234, 388
Esp3I CGTCTC 1 cut(s) 118
FaeI CATG 7 cut(s) 97, 238, 290, 392, 529, 580, 622
FalI AAGNNNNNCTT 2 cut(s) 288, 320
FaqI GGGAC 2 cut(s) 352, 766
FatI CATG 7 cut(s) 93, 234, 286, 388, 525, 576, 618
FauI CCCGC 2 cut(s) 114, 184
FauNDI CATATG 1 cut(s) 492
FbaI TGATCA 1 cut(s) 157
Fnu4HI GCNGC 4 cut(s) 20, 164, 246, 424
FokI GGATG 1 cut(s) 523
FriOI GRGCYC 1 cut(s) 490
Fsp4HI GCNGC 4 cut(s) 20, 164, 246, 424
GluI GCNGC 4 cut(s) 20, 164, 246, 424
GsuI CTGGAG 1 cut(s) 555
HaeIII GGCC 2 cut(s) 499, 505
HapII CCGG 2 cut(s) 83, 770
Hin1II CATG 7 cut(s) 97, 238, 290, 392, 529, 580, 622
HinfI GANTC 2 cut(s) 326, 460
HpaII CCGG 2 cut(s) 83, 770
HphI GGTGA 3 cut(s) 223, 404, 478
Hpy166II GTNNAC 1 cut(s) 231
Hpy188I TCNGA 7 cut(s) 126, 157, 259, 325, 334, 574, 651
Hpy188III TCNNGA 2 cut(s) 43, 94
Hpy8I GTNNAC 1 cut(s) 231
HpyAV CCTTC 3 cut(s) 329, 559, 586
HpyCH4IV ACGT 4 cut(s) 300, 341, 653, 755
HpyCH4V TGCA 2 cut(s) 286, 367
HpyF3I CTNAG 1 cut(s) 324
HpySE526I ACGT 4 cut(s) 300, 341, 653, 755
Hsp92II CATG 7 cut(s) 97, 238, 290, 392, 529, 580, 622
Ksp22I TGATCA 1 cut(s) 157
KspI CCGCGG 1 cut(s) 601
Kzo9I GATC 3 cut(s) 157, 666, 715
LmnI GCTCC 2 cut(s) 536, 740
Lsp1109I GCAGC 2 cut(s) 175, 232
MaeII ACGT 4 cut(s) 300, 341, 653, 755
MaeIII GTNAC 1 cut(s) 275
MalI GATC 3 cut(s) 159, 668, 717
MbiI CCGCTC 1 cut(s) 109
MboI GATC 3 cut(s) 157, 666, 715
MboII GAAGA 7 cut(s) 162, 296, 308, 460, 623, 793, 800
MflI RGATCY 1 cut(s) 715
MhlI GDGCHC 1 cut(s) 490
MluCI AATT 2 cut(s) 371, 692
MlyI GAGTC 1 cut(s) 335
MnlI CCTC 7 cut(s) 101, 151, 157, 211, 346, 351, 489
Mph1103I ATGCAT 1 cut(s) 288
MroXI GAANNNNTTC 2 cut(s) 300, 797
MseI TTAA 2 cut(s) 624, 656
MspA1I CMGCKG 2 cut(s) 179, 600
MspI CCGG 2 cut(s) 83, 770
MspR9I CCNGG 3 cut(s) 502, 507, 534
MvaI CCWGG 3 cut(s) 502, 507, 534
MvnI CGCG 2 cut(s) 426, 600
NcoI CCATGG 2 cut(s) 234, 388
NdeI CATATG 1 cut(s) 492
NdeII GATC 3 cut(s) 157, 666, 715
NlaIII CATG 7 cut(s) 97, 238, 290, 392, 529, 580, 622
NmeAIII GCCGAG 1 cut(s) 241
NsiI ATGCAT 1 cut(s) 288
NspI RCATGY 1 cut(s) 290
PagI TCATGA 1 cut(s) 93
PcsI WCGNNNNNNNCGW 1 cut(s) 338
PdmI GAANNNNTTC 2 cut(s) 300, 797
PfeI GAWTC 1 cut(s) 460
PfoI TCCNGGA 1 cut(s) 532
PkrI GCNGC 4 cut(s) 21, 165, 247, 425
PleI GAGTC 1 cut(s) 334
PpsI GAGTC 1 cut(s) 334
Psp6I CCWGG 3 cut(s) 500, 505, 532
PspGI CCWGG 3 cut(s) 500, 505, 532
PspPI GGNCC 4 cut(s) 10, 432, 498, 595
PsuI RGATCY 1 cut(s) 715
RsaI GTAC 1 cut(s) 267
RsaNI GTAC 1 cut(s) 266
SacII CCGCGG 1 cut(s) 601
SaqAI TTAA 2 cut(s) 624, 656
SatI GCNGC 4 cut(s) 20, 164, 246, 424
Sau3AI GATC 3 cut(s) 157, 666, 715
Sau96I GGNCC 4 cut(s) 10, 432, 498, 595
SchI GAGTC 1 cut(s) 335
ScrFI CCNGG 3 cut(s) 502, 507, 534
SduI GDGCHC 1 cut(s) 490
Sfr303I CCGCGG 1 cut(s) 601
SgrBI CCGCGG 1 cut(s) 601
SinI GGWCC 3 cut(s) 10, 432, 595
SmlI CTYRAG 2 cut(s) 401, 710
SmoI CTYRAG 2 cut(s) 401, 710
Sse9I AATT 2 cut(s) 371, 692
SsiI CCGC 8 cut(s) 19, 107, 144, 150, 177, 424, 598, 600
StyD4I CCNGG 3 cut(s) 500, 505, 532
StyI CCWWGG 2 cut(s) 234, 388
TaiI ACGT 4 cut(s) 303, 344, 656, 758
TasI AATT 2 cut(s) 371, 692
TauI GCSGC 2 cut(s) 22, 426
TfiI GAWTC 1 cut(s) 460
Tru1I TTAA 2 cut(s) 624, 656
Tru9I TTAA 2 cut(s) 624, 656
TseI GCWGC 2 cut(s) 163, 245
TspDTI ATGAA 6 cut(s) 110, 395, 461, 485, 514, 593
TspGWI ACGGA 2 cut(s) 304, 337
VpaK11BI GGWCC 3 cut(s) 10, 432, 595
XapI RAATTY 1 cut(s) 371
XceI RCATGY 1 cut(s) 290
XmnI GAANNNNTTC 2 cut(s) 300, 797
Zsp2I ATGCAT 1 cut(s) 288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.