Prupe.2G215900_v2.0.a1

General transcription factor IIF subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0013034
Physical Location & Seq
Reverse (-)
24653799 .. 24657154
3356 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G215900.1

Sequence Viewer

Length: 837 bp
ATGGAAGACCAAAAGCAACAGCAGCAACAAGGCAGCAACAACAACAATGCCAATGGGCCTTTGGAGACGACCAGAGCAGAGCGAGCTGTTTGGCTCATGAAATGCCCACCGCTTGTCGCTAAAAGTCTCAAGCCTGACGCCTCCTCCACTTCTGCCTCTGCTTCTTCTCCATCTGATCAGCAGTCTTCTCGCCCTGTGGCCAAGGTCGTTCTCTCCATTGACCCTCTTAACTCCAATGACGACTCTTCCTCTCCTCAGTTCACCATGGAACTGGCGGCCACTGAATCTGGAAATATGCCCAAGTGTTACTCTTTGGATATGTCTAAAGACTTCATTCCAATGTCTGTGTTTTCTGAGTTATCACAAGGAAAGTTATCTGTGGAGGGGAAAATTTTGAACAAATTTGATATGAAGCCCCATAATGAGAACCTTGAGAATTATGGGAAACTTTGCCGTGAACGGACGAAAAAGTATATGAAAAACAGAAAGATTCAGGTGATTGACAATGACAATGGGGCTCATATGAGGCCTATGCCAGGGATGATTGGTTTTATAGCTCCTGGACCCAGTGAAAAGAAGAAAATGCCAACAAAGGGGTCAGACACGAAAAGAACAAGGAGGGACCGTGGAGAAATGGAAGCCATTATGTTTAAGCTCTTCGAAAGGCAACCAAATTGGACGTTAAGACAACTGATCCAAGAAACAGATCAACCAGAACAATTCTTGAAAGACATACTTAAGGAACTTTGTGTTTACAACAACAAGGGAGCTAATCAAGGGACGTATGAGCTGAAACCAGAATACAGGAAATCTGTCGAGGAGCCAACTCCCGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000375 GO:0000377 GO:0000398 GO:0000428 GO:0000988 GO:0000990 GO:0000991 GO:0003674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0005674 GO:0005856 GO:0006139 GO:0006351 GO:0006352 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006367 GO:0006368 GO:0006370 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0008150 GO:0008152 GO:0008380 GO:0008543 GO:0009058 GO:0009059 GO:0009301 GO:0009452 GO:0009719 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0015630 GO:0016070 GO:0016071 GO:0016073 GO:0016591 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0023052 GO:0030880 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031334 GO:0031974 GO:0031981 GO:0032774 GO:0032784 GO:0032786 GO:0032968 GO:0032991 GO:0032993 GO:0034243 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0036260 GO:0042221 GO:0042795 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044344 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044798 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051716 GO:0055029 GO:0060255 GO:0060260 GO:0060261 GO:0061695 GO:0065007 GO:0070013 GO:0070848 GO:0070887 GO:0071310 GO:0071363 GO:0071495 GO:0071704 GO:0071774 GO:0080090 GO:0090304 GO:0090575 GO:0097550 GO:0097659 GO:0098781 GO:0140110 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1902680 GO:1903506 GO:1903508 GO:1990234 GO:2000112 GO:2000142 GO:2000144 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03022 map03022
Pfam Domains
Protein Families
TFIIF_beta

Protein Analysis

279

Amino Acids

31.43

Weight (kDa)

8.72

Isoelectric Point (pI)

46.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013034)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52270
fragaria_vesca FvH4_7g19881
malus_domestica MD01G1111000.v1.1 MD07G1177200.v1.1
prunus_persica Prupe.2G215900_v2.0.a1
pyrus_communis pycom07g17120
rosa_chinensis RchiOBHm_Chr1g0363871
rosa_laevigata RLG00000027539
rosa_multiflora Rmu_sc0002483.1_g000003
rosa_roxburghii Rroxscaffold_4G00292900
rosa_rugosa Rorug01G0310400 Rorug01G0310500 Rorug01G0310600
rosa_samantha Rh1AG318500 Rh1BG281700 Rh1CG298100 Rh1DG313000
rosa_wichuraiana Rw1G028270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 110, 275
AclWI GGATC 1 cut(s) 688
AcoI YGGCCR 2 cut(s) 198, 276
AcsI RAATTY 2 cut(s) 390, 401
AcyI GRCGYC 1 cut(s) 138
AfiI CCNNNNNNNGG 2 cut(s) 536, 593
AflII CTTAAG 1 cut(s) 737
AgsI TTSAA 2 cut(s) 397, 727
AjnI CCWGG 2 cut(s) 535, 559
AluBI AGCT 5 cut(s) 86, 557, 655, 770, 790
AluI AGCT 5 cut(s) 86, 557, 655, 770, 790
Alw26I GTCTC 2 cut(s) 59, 131
AlwI GGATC 1 cut(s) 688
AoxI GGCC 4 cut(s) 56, 198, 276, 527
ApeKI GCWGC 2 cut(s) 22, 33
ApoI RAATTY 2 cut(s) 390, 401
AspS9I GGNCC 3 cut(s) 56, 563, 622
AsuHPI GGTGA 2 cut(s) 253, 508
AsuII TTCGAA 1 cut(s) 660
AvaII GGWCC 2 cut(s) 563, 622
BalI TGGCCA 1 cut(s) 200
BanII GRGCYC 1 cut(s) 520
BbsI GAAGAC 2 cut(s) 12, 177
BbvI GCAGC 2 cut(s) 34, 45
BccI CCATC 1 cut(s) 178
BceAI ACGGC 1 cut(s) 438
BcgI CGANNNNNNTGC 2 cut(s) 170, 204
BciT130I CCWGG 2 cut(s) 537, 561
BclI TGATCA 1 cut(s) 175
BcoDI GTCTC 2 cut(s) 59, 131
BfrI CTTAAG 1 cut(s) 737
BisI GCNGC 3 cut(s) 23, 34, 276
BlsI GCNGC 3 cut(s) 24, 35, 277
Bme1390I CCNGG 2 cut(s) 537, 561
Bme18I GGWCC 2 cut(s) 563, 622
BmgT120I GGNCC 3 cut(s) 56, 563, 622
BmiI GGNNCC 3 cut(s) 565, 623, 822
BmrFI CCNGG 2 cut(s) 537, 561
BmrI ACTGGG 1 cut(s) 561
BmuI ACTGGG 1 cut(s) 561
BpiI GAAGAC 2 cut(s) 12, 177
Bpu14I TTCGAA 1 cut(s) 660
BpuEI CTTGAG 2 cut(s) 113, 452
BsaHI GRCGYC 1 cut(s) 138
BsaJI CCNNGG 4 cut(s) 201, 264, 536, 625
BsaXI ACNNNNNCTCC 4 cut(s) 128, 158, 610, 640
Bsc4I CCNNNNNNNGG 2 cut(s) 536, 593
Bse1I ACTGG 2 cut(s) 276, 567
BseBI CCWGG 2 cut(s) 537, 561
BseDI CCNNGG 4 cut(s) 201, 264, 536, 625
BseGI GGATG 1 cut(s) 546
BseLI CCNNNNNNNGG 2 cut(s) 536, 593
BseMII CTCAG 2 cut(s) 269, 345
BseNI ACTGG 2 cut(s) 276, 567
BseRI GAGGAG 3 cut(s) 133, 243, 833
BseXI GCAGC 2 cut(s) 34, 45
BshFI GGCC 4 cut(s) 58, 200, 278, 529
BslFI GGGAC 2 cut(s) 635, 793
BslI CCNNNNNNNGG 2 cut(s) 536, 593
BsmAI GTCTC 2 cut(s) 59, 131
BsmBI CGTCTC 1 cut(s) 59
BsmFI GGGAC 2 cut(s) 635, 793
BsnI GGCC 4 cut(s) 58, 200, 278, 529
Bsp119I TTCGAA 1 cut(s) 660
Bsp1286I GDGCHC 1 cut(s) 520
Bsp143I GATC 3 cut(s) 175, 693, 706
Bsp19I CCATGG 1 cut(s) 264
BspACI CCGC 2 cut(s) 110, 275
BspANI GGCC 4 cut(s) 58, 200, 278, 529
BspCNI CTCAG 2 cut(s) 268, 346
BspHI TCATGA 1 cut(s) 96
BspLI GGNNCC 3 cut(s) 565, 623, 822
BspPI GGATC 1 cut(s) 688
BspQI GCTCTTC 1 cut(s) 662
BspT104I TTCGAA 1 cut(s) 660
BspTI CTTAAG 1 cut(s) 737
BsrI ACTGG 2 cut(s) 276, 567
BssECI CCNNGG 4 cut(s) 201, 264, 536, 625
BssMI GATC 3 cut(s) 175, 693, 706
BssNI GRCGYC 1 cut(s) 138
BssT1I CCWWGG 2 cut(s) 201, 264
Bst2UI CCWGG 2 cut(s) 537, 561
Bst4CI ACNGT 1 cut(s) 626
Bst6I CTCTTC 2 cut(s) 250, 662
BstACI GRCGYC 1 cut(s) 138
BstAFI CTTAAG 1 cut(s) 737
BstBI TTCGAA 1 cut(s) 660
BstC8I GCNNGC 1 cut(s) 84
BstDEI CTNAG 2 cut(s) 255, 354
BstDSI CCRYGG 2 cut(s) 264, 625
BstENI CCTNNNNNAGG 1 cut(s) 534
BstF5I GGATG 1 cut(s) 546
BstKTI GATC 3 cut(s) 178, 696, 709
BstMAI GTCTC 2 cut(s) 59, 131
BstMBI GATC 3 cut(s) 175, 693, 706
BstMWI GCNNNNNNNGC 2 cut(s) 22, 83
BstNI CCWGG 2 cut(s) 537, 561
BstSCI CCNGG 2 cut(s) 535, 559
BstV1I GCAGC 2 cut(s) 34, 45
BstV2I GAAGAC 2 cut(s) 12, 177
BstXI CCANNNNNNTGG 1 cut(s) 271
BsuRI GGCC 4 cut(s) 58, 200, 278, 529
BtgI CCRYGG 2 cut(s) 264, 625
BtsCI GGATG 1 cut(s) 546
BtsIMutI CAGTG 2 cut(s) 279, 574
Cac8I GCNNGC 1 cut(s) 84
CciI TCATGA 1 cut(s) 96
Cfr13I GGNCC 3 cut(s) 56, 563, 622
CseI GACGC 1 cut(s) 146
CviAII CATG 2 cut(s) 97, 265
DdeI CTNAG 2 cut(s) 255, 354
DpnI GATC 3 cut(s) 177, 695, 708
DpnII GATC 3 cut(s) 175, 693, 706
EaeI YGGCCR 2 cut(s) 198, 276
Eam1104I CTCTTC 2 cut(s) 250, 662
EarI CTCTTC 2 cut(s) 250, 662
Eco130I CCWWGG 2 cut(s) 201, 264
Eco147I AGGCCT 1 cut(s) 529
Eco24I GRGCYC 1 cut(s) 520
Eco47I GGWCC 2 cut(s) 563, 622
EcoNI CCTNNNNNAGG 1 cut(s) 534
EcoRII CCWGG 2 cut(s) 535, 559
EcoT14I CCWWGG 2 cut(s) 201, 264
EcoT38I GRGCYC 1 cut(s) 520
ErhI CCWWGG 2 cut(s) 201, 264
Esp3I CGTCTC 1 cut(s) 59
FaeI CATG 2 cut(s) 100, 268
FalI AAGNNNNNCTT 2 cut(s) 720, 752
FaqI GGGAC 2 cut(s) 635, 793
FatI CATG 2 cut(s) 96, 264
FauNDI CATATG 1 cut(s) 522
FbaI TGATCA 1 cut(s) 175
Fnu4HI GCNGC 3 cut(s) 23, 34, 276
FokI GGATG 1 cut(s) 553
FriOI GRGCYC 1 cut(s) 520
Fsp4HI GCNGC 3 cut(s) 23, 34, 276
GluI GCNGC 3 cut(s) 23, 34, 276
HaeIII GGCC 4 cut(s) 58, 200, 278, 529
HgaI GACGC 1 cut(s) 146
Hin1I GRCGYC 1 cut(s) 138
Hin1II CATG 2 cut(s) 100, 268
HinfI GANTC 3 cut(s) 242, 284, 490
HphI GGTGA 2 cut(s) 253, 508
Hpy166II GTNNAC 3 cut(s) 261, 458, 754
Hpy188I TCNGA 3 cut(s) 175, 355, 601
Hpy188III TCNNGA 4 cut(s) 97, 288, 724, 830
Hpy8I GTNNAC 3 cut(s) 261, 458, 754
HpyCH4III ACNGT 1 cut(s) 626
HpyCH4IV ACGT 2 cut(s) 680, 782
HpyF10VI GCNNNNNNNGC 2 cut(s) 22, 83
HpyF3I CTNAG 2 cut(s) 255, 354
HpySE526I ACGT 2 cut(s) 680, 782
Hsp92I GRCGYC 1 cut(s) 138
Hsp92II CATG 2 cut(s) 100, 268
Ksp22I TGATCA 1 cut(s) 175
Kzo9I GATC 3 cut(s) 175, 693, 706
LguI GCTCTTC 1 cut(s) 662
LmnI GCTCC 3 cut(s) 562, 767, 820
Lsp1109I GCAGC 2 cut(s) 34, 45
MaeII ACGT 2 cut(s) 680, 782
MaeIII GTNAC 1 cut(s) 305
MalI GATC 3 cut(s) 177, 695, 708
MboI GATC 3 cut(s) 175, 693, 706
MboII GAAGA 6 cut(s) 17, 156, 177, 237, 589, 649
MhlI GDGCHC 1 cut(s) 520
MlsI TGGCCA 1 cut(s) 200
MluCI AATT 5 cut(s) 390, 401, 436, 673, 719
MluNI TGGCCA 1 cut(s) 200
MlyI GAGTC 1 cut(s) 236
Mox20I TGGCCA 1 cut(s) 200
MscI TGGCCA 1 cut(s) 200
MseI TTAA 4 cut(s) 228, 651, 683, 738
MslI CAYNNNNRTG 1 cut(s) 338
Msp20I TGGCCA 1 cut(s) 200
MspCI CTTAAG 1 cut(s) 737
MspR9I CCNGG 2 cut(s) 537, 561
MvaI CCWGG 2 cut(s) 537, 561
MwoI GCNNNNNNNGC 2 cut(s) 22, 83
NcoI CCATGG 1 cut(s) 264
NdeI CATATG 1 cut(s) 522
NdeII GATC 3 cut(s) 175, 693, 706
NlaIII CATG 2 cut(s) 100, 268
NlaIV GGNNCC 3 cut(s) 565, 623, 822
NspV TTCGAA 1 cut(s) 660
PagI TCATGA 1 cut(s) 96
PceI AGGCCT 1 cut(s) 529
PciSI GCTCTTC 1 cut(s) 662
PfeI GAWTC 2 cut(s) 284, 490
PfoI TCCNGGA 1 cut(s) 559
PkrI GCNGC 3 cut(s) 24, 35, 277
PleI GAGTC 1 cut(s) 236
PpsI GAGTC 1 cut(s) 236
Psp6I CCWGG 2 cut(s) 535, 559
PspGI CCWGG 2 cut(s) 535, 559
PspN4I GGNNCC 3 cut(s) 565, 623, 822
PspPI GGNCC 3 cut(s) 56, 563, 622
RseI CAYNNNNRTG 1 cut(s) 338
SapI GCTCTTC 1 cut(s) 662
SaqAI TTAA 4 cut(s) 228, 651, 683, 738
SatI GCNGC 3 cut(s) 23, 34, 276
Sau3AI GATC 3 cut(s) 175, 693, 706
Sau96I GGNCC 3 cut(s) 56, 563, 622
SchI GAGTC 1 cut(s) 236
ScrFI CCNGG 2 cut(s) 537, 561
SduI GDGCHC 1 cut(s) 520
SfuI TTCGAA 1 cut(s) 660
SinI GGWCC 2 cut(s) 563, 622
SmiMI CAYNNNNRTG 1 cut(s) 338
SmlI CTYRAG 3 cut(s) 128, 431, 737
SmoI CTYRAG 3 cut(s) 128, 431, 737
Sse9I AATT 5 cut(s) 390, 401, 436, 673, 719
SseBI AGGCCT 1 cut(s) 529
SsiI CCGC 2 cut(s) 110, 275
StuI AGGCCT 1 cut(s) 529
StyD4I CCNGG 2 cut(s) 535, 559
StyI CCWWGG 2 cut(s) 201, 264
TaaI ACNGT 1 cut(s) 626
TaiI ACGT 2 cut(s) 683, 785
TaqI TCGA 2 cut(s) 660, 816
TasI AATT 5 cut(s) 390, 401, 436, 673, 719
TauI GCSGC 1 cut(s) 278
TfiI GAWTC 2 cut(s) 284, 490
Tru1I TTAA 4 cut(s) 228, 651, 683, 738
Tru9I TTAA 4 cut(s) 228, 651, 683, 738
TscAI CASTG 2 cut(s) 286, 574
TseI GCWGC 2 cut(s) 22, 33
TspDTI ATGAA 4 cut(s) 113, 322, 425, 491
TspGWI ACGGA 1 cut(s) 475
TspRI CASTG 2 cut(s) 286, 574
Vha464I CTTAAG 1 cut(s) 737
VpaK11BI GGWCC 2 cut(s) 563, 622
XagI CCTNNNNNAGG 1 cut(s) 534
XapI RAATTY 2 cut(s) 390, 401
XcmI CCANNNNNNNNNTGG 1 cut(s) 58
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.