Rorug01G0310500

General transcription factor IIF subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0013034
Physical Location & Seq
Forward (+)
42722813 .. 42725968
3156 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0310500.1

Sequence Viewer

Length: 990 bp
ATGGCTGACAAGTACGGACCGCTCTTCACTATCAAGCTTGGTGTGCACCGAGCTCTTGTTGTAAGCAGTTGGGATGTGGCTAAAGAATGTCTCACCACTAATGATAAAGCTTTTGCCAACCGCCCGAAAGCTTTAGGCCCGGAGATTATGGGGTACGACTATGCCATGGTCGGTTTTAGCCCTTACGGACCATACTGGCGCCAGGTGCGAAAGATAACCACGCTGCACATCCTCTCCAACCACAAGTTGGAGTTGCTCAAGCACGTCCGAGAATCGGAGGTGAAGATCTCTACCAAACATATATTTGATATGTGGGAGCGAAACAAAGGTGTCTCGAATAAGGTGCTGGTAGACATGAAGAGGTGGTTTGGAGACCTTACTTCAAAGGTCATGTTCAGGATGGTTGTTGGAAAGCAATTTCGAATTGGGGCTAACTTGGCGCAAGAGAATGAAGAGAATGAAAGCTTTCGAAAGGCGTTGAGGGAATTTCTTAGACTGGTTGGCGAATTTGTAATCTCAGATGCAGTTCCATTTCTTAGGTGGTTGGATTTGGGAGGTTATGAGAAAGCCATGAAGAAGACAGCTAAAGAACTCGATGATGCGCTTCAAAAATGGTTAGAAGAGCATAAGCACAAGAGAAGTACTAATTCTTTGCAACATGATTGTGAGCGTGACTTCATGGATATGATGCTTACCGTCCTTGATGATGATAGTAGAGAAGAGTTTGCTGGTTCAATTACTGCTGATACAATTAACAAAGCTACATGCCTGTTGGTACAGATGCCCGTACTGAAAGTGGTCATGGTGTTCCAAAGCCGGTCCAACATAATGCTCCTTAGCTTGGAGAGAGACACATATTTACTAATTGCTTCGAGTTGTAAAAAAAGCGCAAGCAGCAATATAATAGGCAATGCAAAGCTTAGAATTTTGCGAATTGCCTCATCAAAACATACTGTTTGTAAAGGCAATGCTTCTAGGAGGGACAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000375 GO:0000377 GO:0000398 GO:0000428 GO:0000988 GO:0000990 GO:0000991 GO:0003674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0005674 GO:0005856 GO:0006139 GO:0006351 GO:0006352 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006367 GO:0006368 GO:0006370 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0008150 GO:0008152 GO:0008380 GO:0008543 GO:0009058 GO:0009059 GO:0009301 GO:0009452 GO:0009719 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0015630 GO:0016070 GO:0016071 GO:0016073 GO:0016591 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0023052 GO:0030880 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031334 GO:0031974 GO:0031981 GO:0032774 GO:0032784 GO:0032786 GO:0032968 GO:0032991 GO:0032993 GO:0034243 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0036260 GO:0042221 GO:0042795 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044344 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044798 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051716 GO:0055029 GO:0060255 GO:0060260 GO:0060261 GO:0061695 GO:0065007 GO:0070013 GO:0070848 GO:0070887 GO:0071310 GO:0071363 GO:0071495 GO:0071704 GO:0071774 GO:0080090 GO:0090304 GO:0090575 GO:0097550 GO:0097659 GO:0098781 GO:0140110 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1902680 GO:1903506 GO:1903508 GO:1990234 GO:2000112 GO:2000142 GO:2000144 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03022 map03022
Pfam Domains
Protein Families
TFIIF_beta

Protein Analysis

329

Amino Acids

37.74

Weight (kDa)

9.46

Isoelectric Point (pI)

49.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 2 - 253 3.9e-23 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013034)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52270
fragaria_vesca FvH4_7g19881
malus_domestica MD01G1111000.v1.1 MD07G1177200.v1.1
prunus_persica Prupe.2G215900_v2.0.a1
pyrus_communis pycom07g17120
rosa_chinensis RchiOBHm_Chr1g0363871
rosa_laevigata RLG00000027539
rosa_multiflora Rmu_sc0002483.1_g000003
rosa_roxburghii Rroxscaffold_4G00292900
rosa_rugosa Rorug01G0310400 Rorug01G0310500 Rorug01G0310600
rosa_samantha Rh1AG318500 Rh1BG281700 Rh1CG298100 Rh1DG313000
rosa_wichuraiana Rw1G028270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 198
AccB7I CCANNNNNTGG 1 cut(s) 247
AccBSI CCGCTC 1 cut(s) 22
AccI GTMKAC 1 cut(s) 351
AciI CCGC 2 cut(s) 20, 121
AcsI RAATTY 3 cut(s) 485, 506, 924
AcyI GRCGYC 1 cut(s) 199
AfaI GTAC 5 cut(s) 14, 155, 643, 777, 789
AfiI CCNNNNNNNGG 3 cut(s) 247, 274, 841
AgsI TTSAA 3 cut(s) 384, 608, 735
AjiI CACGTC 1 cut(s) 265
AjnI CCWGG 1 cut(s) 201
AluBI AGCT 9 cut(s) 37, 53, 110, 131, 465, 584, 761, 840, 919
AluI AGCT 9 cut(s) 37, 53, 110, 131, 465, 584, 761, 840, 919
Alw21I GWGCWC 2 cut(s) 48, 55
Alw26I GTCTC 4 cut(s) 95, 337, 366, 843
Alw44I GTGCAC 1 cut(s) 44
AoxI GGCC 1 cut(s) 136
ApaLI GTGCAC 1 cut(s) 44
ApeKI GCWGC 2 cut(s) 223, 894
ApoI RAATTY 3 cut(s) 485, 506, 924
Asp700I GAANNNNTTC 1 cut(s) 465
AspLEI GCGC 4 cut(s) 201, 442, 604, 890
AspS9I GGNCC 4 cut(s) 17, 137, 188, 819
AsuC2I CCSGG 1 cut(s) 140
AsuHPI GGTGA 2 cut(s) 85, 292
AsuII TTCGAA 2 cut(s) 421, 469
AvaII GGWCC 3 cut(s) 17, 188, 819
BaeGI GKGCMC 1 cut(s) 48
BanI GGYRCC 1 cut(s) 198
BanII GRGCYC 1 cut(s) 55
BbsI GAAGAC 1 cut(s) 584
Bbv12I GWGCWC 2 cut(s) 48, 55
BbvI GCAGC 2 cut(s) 210, 906
BccI CCATC 1 cut(s) 394
BcgI CGANNNNNNTGC 2 cut(s) 325, 359
BciT130I CCWGG 1 cut(s) 203
BcnI CCSGG 1 cut(s) 140
BcoDI GTCTC 4 cut(s) 95, 337, 366, 843
BfaI CTAG 1 cut(s) 975
BfoI RGCGCY 1 cut(s) 202
BglII AGATCT 1 cut(s) 285
BisI GCNGC 2 cut(s) 224, 895
BlsI GCNGC 2 cut(s) 225, 896
BmcAI AGTACT 1 cut(s) 643
Bme1390I CCNGG 2 cut(s) 140, 203
Bme18I GGWCC 3 cut(s) 17, 188, 819
BmgBI CACGTC 1 cut(s) 265
BmgT120I GGNCC 4 cut(s) 17, 137, 188, 819
BmiI GGNNCC 1 cut(s) 200
BmrFI CCNGG 2 cut(s) 140, 203
BmsI GCATC 4 cut(s) 511, 589, 678, 771
BpiI GAAGAC 1 cut(s) 584
Bpu10I CCTNAGC 1 cut(s) 836
Bpu14I TTCGAA 2 cut(s) 421, 469
BpuEI CTTGAG 1 cut(s) 242
BpuMI CCSGG 1 cut(s) 140
BsaHI GRCGYC 1 cut(s) 199
BsaI GGTCTC 1 cut(s) 366
BsaJI CCNNGG 1 cut(s) 165
BsaXI ACNNNNNCTCC 2 cut(s) 218, 248
Bsc4I CCNNNNNNNGG 3 cut(s) 247, 274, 841
Bse118I RCCGGY 1 cut(s) 816
Bse1I ACTGG 2 cut(s) 200, 501
Bse3DI GCAATG 2 cut(s) 916, 973
BseBI CCWGG 1 cut(s) 203
BseDI CCNNGG 1 cut(s) 165
BseGI GGATG 3 cut(s) 79, 228, 405
BseLI CCNNNNNNNGG 3 cut(s) 247, 274, 841
BseMI GCAATG 2 cut(s) 916, 973
BseMII CTCAG 1 cut(s) 531
BseNI ACTGG 2 cut(s) 200, 501
BseSI GKGCMC 1 cut(s) 48
BseXI GCAGC 2 cut(s) 210, 906
BsgI GTGCAG 1 cut(s) 209
BshFI GGCC 1 cut(s) 138
BshNI GGYRCC 1 cut(s) 198
BsiHKAI GWGCWC 2 cut(s) 48, 55
BsiSI CCGG 2 cut(s) 140, 817
BslI CCNNNNNNNGG 3 cut(s) 247, 274, 841
BsmAI GTCTC 4 cut(s) 95, 337, 366, 843
BsnI GGCC 1 cut(s) 138
Bso31I GGTCTC 1 cut(s) 366
Bsp119I TTCGAA 2 cut(s) 421, 469
Bsp1286I GDGCHC 2 cut(s) 48, 55
Bsp143I GATC 1 cut(s) 285
Bsp19I CCATGG 1 cut(s) 165
BspACI CCGC 2 cut(s) 20, 121
BspANI GGCC 1 cut(s) 138
BspCNI CTCAG 1 cut(s) 530
BspLI GGNNCC 1 cut(s) 200
BspQI GCTCTTC 2 cut(s) 29, 615
BspT104I TTCGAA 2 cut(s) 421, 469
BspT107I GGYRCC 1 cut(s) 198
BspTNI GGTCTC 1 cut(s) 366
BsrBI CCGCTC 1 cut(s) 22
BsrDI GCAATG 2 cut(s) 916, 973
BsrFI RCCGGY 1 cut(s) 816
BsrI ACTGG 2 cut(s) 200, 501
BssAI RCCGGY 1 cut(s) 816
BssECI CCNNGG 1 cut(s) 165
BssMI GATC 1 cut(s) 285
BssNI GRCGYC 1 cut(s) 199
BssT1I CCWWGG 1 cut(s) 165
Bst2UI CCWGG 1 cut(s) 203
Bst4CI ACNGT 2 cut(s) 697, 955
Bst6I CTCTTC 5 cut(s) 29, 353, 447, 615, 714
BstACI GRCGYC 1 cut(s) 199
BstBI TTCGAA 2 cut(s) 421, 469
BstC8I GCNNGC 1 cut(s) 892
BstDEI CTNAG 5 cut(s) 491, 517, 536, 836, 920
BstDSI CCRYGG 1 cut(s) 165
BstF5I GGATG 3 cut(s) 79, 228, 405
BstH2I RGCGCY 1 cut(s) 202
BstHHI GCGC 4 cut(s) 201, 442, 604, 890
BstKTI GATC 1 cut(s) 288
BstMAI GTCTC 4 cut(s) 95, 337, 366, 843
BstMBI GATC 1 cut(s) 285
BstMWI GCNNNNNNNGC 4 cut(s) 43, 205, 437, 894
BstNI CCWGG 1 cut(s) 203
BstNSI RCATGY 1 cut(s) 768
BstSCI CCNGG 2 cut(s) 138, 201
BstSLI GKGCMC 1 cut(s) 48
BstV1I GCAGC 2 cut(s) 210, 906
BstV2I GAAGAC 1 cut(s) 584
BstX2I RGATCY 1 cut(s) 285
BstYI RGATCY 1 cut(s) 285
BsuRI GGCC 1 cut(s) 138
BtgI CCRYGG 1 cut(s) 165
BtrI CACGTC 1 cut(s) 265
BtsCI GGATG 3 cut(s) 79, 228, 405
Cac8I GCNNGC 1 cut(s) 892
CfoI GCGC 4 cut(s) 201, 442, 604, 890
Cfr10I RCCGGY 1 cut(s) 816
Cfr13I GGNCC 4 cut(s) 17, 137, 188, 819
CpoI CGGWCCG 1 cut(s) 17
Csp6I GTAC 5 cut(s) 13, 154, 642, 776, 788
CspI CGGWCCG 1 cut(s) 17
CviAII CATG 8 cut(s) 166, 355, 391, 571, 659, 679, 765, 802
CviQI GTAC 5 cut(s) 13, 154, 642, 776, 788
DdeI CTNAG 5 cut(s) 491, 517, 536, 836, 920
DinI GGCGCC 1 cut(s) 200
DpnI GATC 1 cut(s) 287
DpnII GATC 1 cut(s) 285
Eam1104I CTCTTC 5 cut(s) 29, 353, 447, 615, 714
EarI CTCTTC 5 cut(s) 29, 353, 447, 615, 714
Ecl136II GAGCTC 1 cut(s) 53
Eco130I CCWWGG 1 cut(s) 165
Eco24I GRGCYC 1 cut(s) 55
Eco31I GGTCTC 1 cut(s) 366
Eco47I GGWCC 3 cut(s) 17, 188, 819
Eco53kI GAGCTC 1 cut(s) 53
EcoICRI GAGCTC 1 cut(s) 53
EcoRII CCWGG 1 cut(s) 201
EcoT14I CCWWGG 1 cut(s) 165
EcoT38I GRGCYC 1 cut(s) 55
EgeI GGCGCC 1 cut(s) 200
EheI GGCGCC 1 cut(s) 200
ErhI CCWWGG 1 cut(s) 165
FaeI CATG 8 cut(s) 169, 358, 394, 574, 662, 682, 768, 805
FatI CATG 8 cut(s) 165, 354, 390, 570, 658, 678, 764, 801
FblI GTMKAC 1 cut(s) 351
Fnu4HI GCNGC 2 cut(s) 224, 895
FokI GGATG 3 cut(s) 86, 215, 412
FriOI GRGCYC 1 cut(s) 55
Fsp4HI GCNGC 2 cut(s) 224, 895
FspBI CTAG 1 cut(s) 975
GlaI GCGC 4 cut(s) 200, 441, 603, 889
GluI GCNGC 2 cut(s) 224, 895
HaeII RGCGCY 1 cut(s) 202
HaeIII GGCC 1 cut(s) 138
HapII CCGG 2 cut(s) 140, 817
HhaI GCGC 4 cut(s) 201, 442, 604, 890
Hin1I GRCGYC 1 cut(s) 199
Hin1II CATG 8 cut(s) 169, 358, 394, 574, 662, 682, 768, 805
Hin6I GCGC 4 cut(s) 199, 440, 602, 888
HinP1I GCGC 4 cut(s) 199, 440, 602, 888
HindIII AAGCTT 5 cut(s) 35, 108, 129, 463, 917
HinfI GANTC 1 cut(s) 272
HpaII CCGG 2 cut(s) 140, 817
HphI GGTGA 2 cut(s) 85, 292
Hpy166II GTNNAC 2 cut(s) 46, 352
Hpy188I TCNGA 3 cut(s) 269, 277, 520
Hpy188III TCNNGA 2 cut(s) 334, 397
Hpy8I GTNNAC 2 cut(s) 46, 352
HpyCH4III ACNGT 2 cut(s) 697, 955
HpyCH4IV ACGT 1 cut(s) 264
HpyCH4V TGCA 5 cut(s) 46, 226, 524, 655, 914
HpyF10VI GCNNNNNNNGC 4 cut(s) 43, 205, 437, 894
HpyF3I CTNAG 5 cut(s) 491, 517, 536, 836, 920
HpySE526I ACGT 1 cut(s) 264
Hsp92I GRCGYC 1 cut(s) 199
Hsp92II CATG 8 cut(s) 169, 358, 394, 574, 662, 682, 768, 805
HspAI GCGC 4 cut(s) 199, 440, 602, 888
KasI GGCGCC 1 cut(s) 198
Kzo9I GATC 1 cut(s) 285
LguI GCTCTTC 2 cut(s) 29, 615
LmnI GCTCC 2 cut(s) 316, 837
Lsp1109I GCAGC 2 cut(s) 210, 906
LweI GCATC 4 cut(s) 511, 589, 678, 771
MaeI CTAG 1 cut(s) 975
MaeII ACGT 1 cut(s) 264
MaeIII GTNAC 1 cut(s) 671
MalI GATC 1 cut(s) 287
MbiI CCGCTC 1 cut(s) 22
MboI GATC 1 cut(s) 285
MboII GAAGA 8 cut(s) 16, 295, 370, 464, 586, 589, 632, 731
MflI RGATCY 1 cut(s) 285
MhlI GDGCHC 2 cut(s) 48, 55
Mly113I GGCGCC 1 cut(s) 199
MmeI TCCRAC 5 cut(s) 228, 261, 388, 525, 846
MnlI CCTC 7 cut(s) 242, 271, 354, 474, 548, 949, 972
MroXI GAANNNNTTC 1 cut(s) 465
MseI TTAA 1 cut(s) 753
MslI CAYNNNNRTG 2 cut(s) 663, 683
MspI CCGG 2 cut(s) 140, 817
MspR9I CCNGG 2 cut(s) 140, 203
MvaI CCWGG 1 cut(s) 203
MwoI GCNNNNNNNGC 4 cut(s) 43, 205, 437, 894
NarI GGCGCC 1 cut(s) 199
NciI CCSGG 1 cut(s) 140
NcoI CCATGG 1 cut(s) 165
NdeII GATC 1 cut(s) 285
NlaIII CATG 8 cut(s) 169, 358, 394, 574, 662, 682, 768, 805
NlaIV GGNNCC 1 cut(s) 200
NmuCI GTSAC 1 cut(s) 671
NspI RCATGY 1 cut(s) 768
NspV TTCGAA 2 cut(s) 421, 469
PciSI GCTCTTC 2 cut(s) 29, 615
PdmI GAANNNNTTC 1 cut(s) 465
PfeI GAWTC 1 cut(s) 272
PflMI CCANNNNNTGG 1 cut(s) 247
PkrI GCNGC 2 cut(s) 225, 896
PluTI GGCGCC 1 cut(s) 202
Psp124BI GAGCTC 1 cut(s) 55
Psp6I CCWGG 1 cut(s) 201
PspGI CCWGG 1 cut(s) 201
PspN4I GGNNCC 1 cut(s) 200
PspPI GGNCC 4 cut(s) 17, 137, 188, 819
PsuI RGATCY 1 cut(s) 285
RsaI GTAC 5 cut(s) 14, 155, 643, 777, 789
RsaNI GTAC 5 cut(s) 13, 154, 642, 776, 788
RseI CAYNNNNRTG 2 cut(s) 663, 683
Rsr2I CGGWCCG 1 cut(s) 17
RsrII CGGWCCG 1 cut(s) 17
SacI GAGCTC 1 cut(s) 55
SapI GCTCTTC 2 cut(s) 29, 615
SaqAI TTAA 1 cut(s) 753
SatI GCNGC 2 cut(s) 224, 895
Sau3AI GATC 1 cut(s) 285
Sau96I GGNCC 4 cut(s) 17, 137, 188, 819
ScaI AGTACT 1 cut(s) 643
ScrFI CCNGG 2 cut(s) 140, 203
SduI GDGCHC 2 cut(s) 48, 55
SfaNI GCATC 4 cut(s) 511, 589, 678, 771
SfoI GGCGCC 1 cut(s) 200
SfuI TTCGAA 2 cut(s) 421, 469
SinI GGWCC 3 cut(s) 17, 188, 819
SmiMI CAYNNNNRTG 2 cut(s) 663, 683
SmlI CTYRAG 1 cut(s) 257
SmoI CTYRAG 1 cut(s) 257
SsiI CCGC 2 cut(s) 20, 121
SspDI GGCGCC 1 cut(s) 198
SspMI CTAG 1 cut(s) 975
SstI GAGCTC 1 cut(s) 55
StyD4I CCNGG 2 cut(s) 138, 201
StyI CCWWGG 1 cut(s) 165
TaaI ACNGT 2 cut(s) 697, 955
TaiI ACGT 1 cut(s) 267
TaqI TCGA 5 cut(s) 335, 421, 469, 594, 872
TatI WGTACW 1 cut(s) 641
TfiI GAWTC 1 cut(s) 272
Tru1I TTAA 1 cut(s) 753
Tru9I TTAA 1 cut(s) 753
TseFI GTSAC 1 cut(s) 671
TseI GCWGC 2 cut(s) 223, 894
Tsp45I GTSAC 1 cut(s) 671
TspDTI ATGAA 5 cut(s) 371, 465, 474, 587, 667
TspGWI ACGGA 2 cut(s) 30, 201
Van91I CCANNNNNTGG 1 cut(s) 247
VneI GTGCAC 1 cut(s) 44
VpaK11BI GGWCC 3 cut(s) 17, 188, 819
XapI RAATTY 3 cut(s) 485, 506, 924
XceI RCATGY 1 cut(s) 768
XcmI CCANNNNNNNNNTGG 2 cut(s) 244, 537
XmiI GTMKAC 1 cut(s) 351
XmnI GAANNNNTTC 1 cut(s) 465
XspI CTAG 1 cut(s) 975
ZrmI AGTACT 1 cut(s) 643
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.