Rorug01G0310600

General transcription factor IIF subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0013034
Physical Location & Seq
Forward (+)
42802043 .. 42804611
2569 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0310600.1

Sequence Viewer

Length: 927 bp
ATGGAGTTTCTGCTTCCATCTTCTATATGTGATCCAACTACCATCTTAGCATTTTTTCTCTTTCTGTTCTCTTTCGTATGGATATCAAAATACTTGCAGAAAAGAGAACCCCCGAAAGCGGCTGGTGCATGGCCAATACTCGGCCACCTTCCCCTCTTAGGAGGGCCTCAACCGCCCCACATAACCCTAGGGAACATGGCTGACAAGTACGGACCGCTCTTCACTATCAAGCTTGGTGTGCACCGAGCTCTTGTTGTAAGCAGTTGGGATGTGGCTAAAGAATGTCTCACCACTAATGATAAAGCTTTTGCCAACCGCCCGAAAGCTTTATACTCAGAGATTATGGGGTACGACTATGCCATATTCGGTCTTAGCCCTTACGGACCTTACTGGCGCCAGATGCGAAAGATAACCACGCTGCACATCCTCTCCAACCACAAGTTGGAGTTGCTCAACCACGTCCGAGAATCGGAGGTGAAGGTGTCAACCAAACATATATTTGATATGCGGGAGCGAAACAAAGGTGTCTCAGATAAGGTGTTGGTGGACATGAAGAGGTGGTTTACAGACTTAACTTTAAATGTCATGTTCAGGATGGTTGTTGGAAAGCGATTTTCTGGGGCTAACTCGGCGCAAGAGAATGAAGAGAATGAATGCTTTCGGAAGGCGTTGAGGGAATTTTTAAGATTGGGTGGCGAATTTGTAATCTCAGATGCAGTTCCATTTCTTAGGTGGTTGGATTGGGGAGGTTATGAGAAAGCCATGAAGAAGACAGCTAAAGAACTCGATGGTGCGCTTCAAAAATGGTTAGAAGAGCATAAGCACAAGAGAAGTACTGATTCTTTGCAACATGATTGTGAGCGTGACTTCATGGATATGATGCTTACCGTCCTTGATGATGATAGCAGAGAAGAGTTTGCTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000375 GO:0000377 GO:0000398 GO:0000428 GO:0000988 GO:0000990 GO:0000991 GO:0003674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0005674 GO:0005856 GO:0006139 GO:0006351 GO:0006352 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006367 GO:0006368 GO:0006370 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0008150 GO:0008152 GO:0008380 GO:0008543 GO:0009058 GO:0009059 GO:0009301 GO:0009452 GO:0009719 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0015630 GO:0016070 GO:0016071 GO:0016073 GO:0016591 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0023052 GO:0030880 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031334 GO:0031974 GO:0031981 GO:0032774 GO:0032784 GO:0032786 GO:0032968 GO:0032991 GO:0032993 GO:0034243 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0036260 GO:0042221 GO:0042795 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044344 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044798 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051716 GO:0055029 GO:0060255 GO:0060260 GO:0060261 GO:0061695 GO:0065007 GO:0070013 GO:0070848 GO:0070887 GO:0071310 GO:0071363 GO:0071495 GO:0071704 GO:0071774 GO:0080090 GO:0090304 GO:0090575 GO:0097550 GO:0097659 GO:0098781 GO:0140110 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1902680 GO:1903506 GO:1903508 GO:1990234 GO:2000112 GO:2000142 GO:2000144 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03022 map03022
Pfam Domains
Protein Families
TFIIF_beta

Protein Analysis

308

Amino Acids

35.56

Weight (kDa)

8.23

Isoelectric Point (pI)

51.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 40 - 298 1.5e-27 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013034)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52270
fragaria_vesca FvH4_7g19881
malus_domestica MD01G1111000.v1.1 MD07G1177200.v1.1
prunus_persica Prupe.2G215900_v2.0.a1
pyrus_communis pycom07g17120
rosa_chinensis RchiOBHm_Chr1g0363871
rosa_laevigata RLG00000027539
rosa_multiflora Rmu_sc0002483.1_g000003
rosa_roxburghii Rroxscaffold_4G00292900
rosa_rugosa Rorug01G0310400 Rorug01G0310500 Rorug01G0310600
rosa_samantha Rh1AG318500 Rh1BG281700 Rh1CG298100 Rh1DG313000
rosa_wichuraiana Rw1G028270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 393
AccB7I CCANNNNNTGG 1 cut(s) 442
AccBSI CCGCTC 1 cut(s) 217
AciI CCGC 5 cut(s) 119, 173, 215, 316, 508
AclWI GGATC 1 cut(s) 26
AcoI YGGCCR 2 cut(s) 131, 142
AcsI RAATTY 2 cut(s) 677, 698
AcyI GRCGYC 1 cut(s) 394
AfaI GTAC 3 cut(s) 209, 350, 835
AfiI CCNNNNNNNGG 5 cut(s) 118, 140, 158, 442, 469
AgsI TTSAA 1 cut(s) 800
AjiI CACGTC 1 cut(s) 460
AluBI AGCT 5 cut(s) 232, 248, 305, 326, 776
AluI AGCT 5 cut(s) 232, 248, 305, 326, 776
Alw21I GWGCWC 2 cut(s) 243, 250
Alw26I GTCTC 2 cut(s) 290, 532
Alw44I GTGCAC 1 cut(s) 239
AlwI GGATC 1 cut(s) 26
AoxI GGCC 3 cut(s) 131, 142, 164
ApaLI GTGCAC 1 cut(s) 239
ApeKI GCWGC 1 cut(s) 418
ApoI RAATTY 2 cut(s) 677, 698
Asp700I GAANNNNTTC 1 cut(s) 657
AspA2I CCTAGG 1 cut(s) 187
AspLEI GCGC 3 cut(s) 396, 634, 796
AspS9I GGNCC 3 cut(s) 164, 212, 383
AsuHPI GGTGA 2 cut(s) 280, 487
AvaII GGWCC 2 cut(s) 212, 383
AvrII CCTAGG 1 cut(s) 187
BaeGI GKGCMC 1 cut(s) 243
BalI TGGCCA 1 cut(s) 133
BanI GGYRCC 1 cut(s) 393
BanII GRGCYC 1 cut(s) 250
BbsI GAAGAC 1 cut(s) 776
Bbv12I GWGCWC 2 cut(s) 243, 250
BbvI GCAGC 1 cut(s) 405
BccI CCATC 4 cut(s) 25, 50, 589, 782
BcoDI GTCTC 2 cut(s) 290, 532
BfaI CTAG 1 cut(s) 188
BfoI RGCGCY 1 cut(s) 397
BisI GCNGC 2 cut(s) 120, 419
BlnI CCTAGG 1 cut(s) 187
BlsI GCNGC 2 cut(s) 121, 420
BmcAI AGTACT 1 cut(s) 835
Bme18I GGWCC 2 cut(s) 212, 383
BmgBI CACGTC 1 cut(s) 460
BmgT120I GGNCC 3 cut(s) 164, 212, 383
BmiI GGNNCC 1 cut(s) 395
BmsI GCATC 3 cut(s) 390, 703, 870
BpiI GAAGAC 1 cut(s) 776
BsaHI GRCGYC 1 cut(s) 394
BsaJI CCNNGG 1 cut(s) 187
BsaXI ACNNNNNCTCC 2 cut(s) 413, 443
Bsc4I CCNNNNNNNGG 5 cut(s) 118, 140, 158, 442, 469
Bse1I ACTGG 1 cut(s) 395
BseDI CCNNGG 1 cut(s) 187
BseGI GGATG 3 cut(s) 274, 423, 600
BseLI CCNNNNNNNGG 5 cut(s) 118, 140, 158, 442, 469
BseMII CTCAG 3 cut(s) 348, 543, 723
BseNI ACTGG 1 cut(s) 395
BseSI GKGCMC 1 cut(s) 243
BseXI GCAGC 1 cut(s) 405
BsgI GTGCAG 1 cut(s) 404
BshFI GGCC 3 cut(s) 133, 144, 166
BshNI GGYRCC 1 cut(s) 393
BsiHKAI GWGCWC 2 cut(s) 243, 250
BslI CCNNNNNNNGG 5 cut(s) 118, 140, 158, 442, 469
BsmAI GTCTC 2 cut(s) 290, 532
BsmI GAATGC 1 cut(s) 659
BsnI GGCC 3 cut(s) 133, 144, 166
Bsp1286I GDGCHC 2 cut(s) 243, 250
Bsp143I GATC 1 cut(s) 31
BspACI CCGC 5 cut(s) 119, 173, 215, 316, 508
BspANI GGCC 3 cut(s) 133, 144, 166
BspCNI CTCAG 3 cut(s) 347, 542, 722
BspLI GGNNCC 1 cut(s) 395
BspPI GGATC 1 cut(s) 26
BspQI GCTCTTC 2 cut(s) 224, 807
BspT107I GGYRCC 1 cut(s) 393
BsrBI CCGCTC 1 cut(s) 217
BsrI ACTGG 1 cut(s) 395
BssECI CCNNGG 1 cut(s) 187
BssMI GATC 1 cut(s) 31
BssNI GRCGYC 1 cut(s) 394
BssT1I CCWWGG 1 cut(s) 187
Bst4CI ACNGT 1 cut(s) 889
Bst6I CTCTTC 5 cut(s) 224, 548, 639, 807, 906
BstACI GRCGYC 1 cut(s) 394
BstDEI CTNAG 7 cut(s) 46, 157, 334, 371, 529, 709, 728
BstF5I GGATG 3 cut(s) 274, 423, 600
BstH2I RGCGCY 1 cut(s) 397
BstHHI GCGC 3 cut(s) 396, 634, 796
BstKTI GATC 1 cut(s) 34
BstMAI GTCTC 2 cut(s) 290, 532
BstMBI GATC 1 cut(s) 31
BstMWI GCNNNNNNNGC 5 cut(s) 125, 172, 238, 400, 629
BstSLI GKGCMC 1 cut(s) 243
BstV1I GCAGC 1 cut(s) 405
BstV2I GAAGAC 1 cut(s) 776
BsuRI GGCC 3 cut(s) 133, 144, 166
BtrI CACGTC 1 cut(s) 460
BtsCI GGATG 3 cut(s) 274, 423, 600
CfoI GCGC 3 cut(s) 396, 634, 796
Cfr13I GGNCC 3 cut(s) 164, 212, 383
CpoI CGGWCCG 1 cut(s) 212
Csp6I GTAC 3 cut(s) 208, 349, 834
CspI CGGWCCG 1 cut(s) 212
CviAII CATG 7 cut(s) 129, 196, 550, 586, 763, 851, 871
CviQI GTAC 3 cut(s) 208, 349, 834
DdeI CTNAG 7 cut(s) 46, 157, 334, 371, 529, 709, 728
DinI GGCGCC 1 cut(s) 395
DpnI GATC 1 cut(s) 33
DpnII GATC 1 cut(s) 31
DraI TTTAAA 1 cut(s) 579
EaeI YGGCCR 2 cut(s) 131, 142
Eam1104I CTCTTC 5 cut(s) 224, 548, 639, 807, 906
EarI CTCTTC 5 cut(s) 224, 548, 639, 807, 906
Ecl136II GAGCTC 1 cut(s) 248
Eco130I CCWWGG 1 cut(s) 187
Eco24I GRGCYC 1 cut(s) 250
Eco32I GATATC 1 cut(s) 84
Eco47I GGWCC 2 cut(s) 212, 383
Eco53kI GAGCTC 1 cut(s) 248
EcoICRI GAGCTC 1 cut(s) 248
EcoO109I RGGNCCY 1 cut(s) 164
EcoRV GATATC 1 cut(s) 84
EcoT14I CCWWGG 1 cut(s) 187
EcoT38I GRGCYC 1 cut(s) 250
EgeI GGCGCC 1 cut(s) 395
EheI GGCGCC 1 cut(s) 395
ErhI CCWWGG 1 cut(s) 187
FaeI CATG 7 cut(s) 132, 199, 553, 589, 766, 854, 874
FatI CATG 7 cut(s) 128, 195, 549, 585, 762, 850, 870
FauI CCCGC 1 cut(s) 501
Fnu4HI GCNGC 2 cut(s) 120, 419
FokI GGATG 3 cut(s) 281, 410, 607
FriOI GRGCYC 1 cut(s) 250
Fsp4HI GCNGC 2 cut(s) 120, 419
FspBI CTAG 1 cut(s) 188
GlaI GCGC 3 cut(s) 395, 633, 795
GluI GCNGC 2 cut(s) 120, 419
HaeII RGCGCY 1 cut(s) 397
HaeIII GGCC 3 cut(s) 133, 144, 166
HhaI GCGC 3 cut(s) 396, 634, 796
Hin1I GRCGYC 1 cut(s) 394
Hin1II CATG 7 cut(s) 132, 199, 553, 589, 766, 854, 874
Hin6I GCGC 3 cut(s) 394, 632, 794
HinP1I GCGC 3 cut(s) 394, 632, 794
HincII GTYRAC 1 cut(s) 486
HindII GTYRAC 1 cut(s) 486
HindIII AAGCTT 3 cut(s) 230, 303, 324
HinfI GANTC 2 cut(s) 467, 839
HphI GGTGA 2 cut(s) 280, 487
Hpy166II GTNNAC 4 cut(s) 241, 486, 547, 564
Hpy188I TCNGA 6 cut(s) 337, 464, 472, 532, 663, 712
Hpy188III TCNNGA 1 cut(s) 592
Hpy8I GTNNAC 4 cut(s) 241, 486, 547, 564
HpyAV CCTTC 3 cut(s) 158, 472, 658
HpyCH4III ACNGT 1 cut(s) 889
HpyCH4IV ACGT 1 cut(s) 459
HpyCH4V TGCA 6 cut(s) 97, 128, 241, 421, 716, 847
HpyF10VI GCNNNNNNNGC 5 cut(s) 125, 172, 238, 400, 629
HpyF3I CTNAG 7 cut(s) 46, 157, 334, 371, 529, 709, 728
HpySE526I ACGT 1 cut(s) 459
Hsp92I GRCGYC 1 cut(s) 394
Hsp92II CATG 7 cut(s) 132, 199, 553, 589, 766, 854, 874
HspAI GCGC 3 cut(s) 394, 632, 794
KasI GGCGCC 1 cut(s) 393
Kzo9I GATC 1 cut(s) 31
LguI GCTCTTC 2 cut(s) 224, 807
LmnI GCTCC 1 cut(s) 511
LpnPI CCDG 6 cut(s) 108, 376, 410, 577, 603, 906
Lsp1109I GCAGC 1 cut(s) 405
LweI GCATC 3 cut(s) 390, 703, 870
MaeI CTAG 1 cut(s) 188
MaeII ACGT 1 cut(s) 459
MaeIII GTNAC 1 cut(s) 863
MalI GATC 1 cut(s) 33
MbiI CCGCTC 1 cut(s) 217
MboI GATC 1 cut(s) 31
MboII GAAGA 8 cut(s) 12, 211, 565, 656, 778, 781, 824, 923
MhlI GDGCHC 2 cut(s) 243, 250
MlsI TGGCCA 1 cut(s) 133
MluCI AATT 2 cut(s) 677, 698
MluNI TGGCCA 1 cut(s) 133
Mly113I GGCGCC 1 cut(s) 394
MmeI TCCRAC 5 cut(s) 59, 423, 456, 583, 717
MnlI CCTC 8 cut(s) 155, 164, 177, 437, 466, 549, 666, 740
Mox20I TGGCCA 1 cut(s) 133
MroXI GAANNNNTTC 1 cut(s) 657
MscI TGGCCA 1 cut(s) 133
MseI TTAA 4 cut(s) 572, 578, 683, 925
MslI CAYNNNNRTG 2 cut(s) 855, 875
Msp20I TGGCCA 1 cut(s) 133
Mva1269I GAATGC 1 cut(s) 659
MwoI GCNNNNNNNGC 5 cut(s) 125, 172, 238, 400, 629
NarI GGCGCC 1 cut(s) 394
NdeII GATC 1 cut(s) 31
NlaIII CATG 7 cut(s) 132, 199, 553, 589, 766, 854, 874
NlaIV GGNNCC 1 cut(s) 395
NmeAIII GCCGAG 2 cut(s) 120, 608
NmuCI GTSAC 1 cut(s) 863
PciSI GCTCTTC 2 cut(s) 224, 807
PctI GAATGC 1 cut(s) 659
PdmI GAANNNNTTC 1 cut(s) 657
PfeI GAWTC 2 cut(s) 467, 839
PflMI CCANNNNNTGG 1 cut(s) 442
PkrI GCNGC 2 cut(s) 121, 420
PluTI GGCGCC 1 cut(s) 397
Psp124BI GAGCTC 1 cut(s) 250
PspN4I GGNNCC 1 cut(s) 395
PspPI GGNCC 3 cut(s) 164, 212, 383
RsaI GTAC 3 cut(s) 209, 350, 835
RsaNI GTAC 3 cut(s) 208, 349, 834
RseI CAYNNNNRTG 2 cut(s) 855, 875
Rsr2I CGGWCCG 1 cut(s) 212
RsrII CGGWCCG 1 cut(s) 212
SacI GAGCTC 1 cut(s) 250
SapI GCTCTTC 2 cut(s) 224, 807
SaqAI TTAA 4 cut(s) 572, 578, 683, 925
SatI GCNGC 2 cut(s) 120, 419
Sau3AI GATC 1 cut(s) 31
Sau96I GGNCC 3 cut(s) 164, 212, 383
ScaI AGTACT 1 cut(s) 835
SduI GDGCHC 2 cut(s) 243, 250
SfaNI GCATC 3 cut(s) 390, 703, 870
SfoI GGCGCC 1 cut(s) 395
SinI GGWCC 2 cut(s) 212, 383
SmiMI CAYNNNNRTG 2 cut(s) 855, 875
Sse9I AATT 2 cut(s) 677, 698
SsiI CCGC 5 cut(s) 119, 173, 215, 316, 508
SspDI GGCGCC 1 cut(s) 393
SspMI CTAG 1 cut(s) 188
SstI GAGCTC 1 cut(s) 250
StyI CCWWGG 1 cut(s) 187
TaaI ACNGT 1 cut(s) 889
TaiI ACGT 1 cut(s) 462
TaqI TCGA 1 cut(s) 786
TaqII GACCGA 1 cut(s) 356
TasI AATT 2 cut(s) 677, 698
TatI WGTACW 1 cut(s) 833
TauI GCSGC 1 cut(s) 122
TfiI GAWTC 2 cut(s) 467, 839
Tru1I TTAA 4 cut(s) 572, 578, 683, 925
Tru9I TTAA 4 cut(s) 572, 578, 683, 925
TseFI GTSAC 1 cut(s) 863
TseI GCWGC 1 cut(s) 418
Tsp45I GTSAC 1 cut(s) 863
TspDTI ATGAA 5 cut(s) 566, 657, 666, 779, 859
TspGWI ACGGA 2 cut(s) 225, 396
Van91I CCANNNNNTGG 1 cut(s) 442
VneI GTGCAC 1 cut(s) 239
VpaK11BI GGWCC 2 cut(s) 212, 383
XapI RAATTY 2 cut(s) 677, 698
XcmI CCANNNNNNNNNTGG 2 cut(s) 439, 729
XmaJI CCTAGG 1 cut(s) 187
XmnI GAANNNNTTC 1 cut(s) 657
XspI CTAG 1 cut(s) 188
ZrmI AGTACT 1 cut(s) 835
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.